RchiOBHm_Chr6g0303531

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003977
Physical Location & Seq
Forward (+)
63245932 .. 63247848
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27269

Sequence Viewer

Length: 447 bp
ATGGCGCGCAGATGCTCTCACGCTTCTCGCGCCGTGATTGCCGAGTTCATCGCCGGCCTTCTGTTTTTGTACATCACAGTCGCTACACGGCATAGTGCTTGGGAGCGATATGTGGAGTTGGGTTGGTCAAGGGGGGTGATGAAGCATTACTATGAAAGCTCACCGGGTAGAGGTGCCAATACTGTGGCTGCTGGCTATGCTAATGGAACCGCTTTGGGAACTGAGATTATCGGAACTTTTGTGCTGGTCTACACCGTCTTCTCTGCCACCGATCCTAAGAGAAACGCGCGAGATTCTCACGTACCTGTTTTGGCCCCATTGCTAATTGGGTTTGCTGTGTTTGTGGTGCACTTGGCCACGATTCCCATCACAGGGACCGGAATCAACCCTGCTAGGAGCTTGGGAGCAGCCGTCATCTTCGACAACCATAAAGTTTGGGACGATTAG

Protein Analysis

148

Amino Acids

16.02

Weight (kDa)

9.25

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 51 - 144 2.6e-27 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 249
AccII CGCG 4 cut(s) 7, 30, 287, 289
AciI CCGC 1 cut(s) 210
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 354
AfaI GTAC 2 cut(s) 71, 303
AfiI CCNNNNNNNGG 3 cut(s) 170, 371, 372
AluBI AGCT 2 cut(s) 159, 399
AluI AGCT 2 cut(s) 159, 399
Alw21I GWGCWC 1 cut(s) 351
Alw44I GTGCAC 1 cut(s) 347
AlwI GGATC 1 cut(s) 266
AoxI GGCC 3 cut(s) 55, 312, 354
ApaLI GTGCAC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 188, 407
AspLEI GCGC 4 cut(s) 7, 9, 32, 289
AspS9I GGNCC 2 cut(s) 313, 375
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 148, 153
AvaII GGWCC 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 351
BalI TGGCCA 1 cut(s) 356
BanI GGYRCC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 175, 419
BccI CCATC 1 cut(s) 374
BceAI ACGGC 3 cut(s) 17, 104, 395
BcnI CCSGG 1 cut(s) 165
BfaI CTAG 1 cut(s) 393
BisI GCNGC 2 cut(s) 189, 408
BlsI GCNGC 2 cut(s) 190, 409
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 1 cut(s) 375
BmgT120I GGNCC 2 cut(s) 313, 375
BmiI GGNNCC 4 cut(s) 175, 208, 315, 376
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 1 cut(s) 2
BpiI GAAGAC 1 cut(s) 250
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 365
BsaWI WCCGGW 1 cut(s) 377
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 371, 372
Bse118I RCCGGY 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 317
Bse8I GATNNNNATC 1 cut(s) 365
BseJI GATNNNNATC 1 cut(s) 365
BseLI CCNNNNNNNGG 3 cut(s) 170, 371, 372
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 1 cut(s) 213
BsePI GCGCGC 1 cut(s) 5
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 2 cut(s) 175, 419
Bsh1236I CGCG 4 cut(s) 7, 30, 287, 289
BshFI GGCC 3 cut(s) 57, 314, 356
BshNI GGYRCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 351
BsiSI CCGG 3 cut(s) 54, 164, 378
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 3 cut(s) 170, 371, 372
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 3 cut(s) 57, 314, 356
Bsp1286I GDGCHC 1 cut(s) 351
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 1 cut(s) 210
BspANI GGCC 3 cut(s) 57, 314, 356
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 4 cut(s) 7, 30, 287, 289
BspLI GGNNCC 4 cut(s) 175, 208, 315, 376
BspPI GGATC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 53
BsrGI TGTACA 1 cut(s) 69
BssAI RCCGGY 1 cut(s) 53
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 1 cut(s) 271
Bst4CI ACNGT 3 cut(s) 79, 184, 256
BstAUI TGTACA 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 3 cut(s) 7, 55, 193
BstDEI CTNAG 2 cut(s) 222, 276
BstFNI CGCG 4 cut(s) 7, 30, 287, 289
BstHHI GCGC 4 cut(s) 7, 9, 32, 289
BstKTI GATC 1 cut(s) 274
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 3 cut(s) 29, 38, 197
BstSCI CCNGG 1 cut(s) 163
BstSLI GKGCMC 1 cut(s) 351
BstUI CGCG 4 cut(s) 7, 30, 287, 289
BstV1I GCAGC 2 cut(s) 175, 419
BstV2I GAAGAC 1 cut(s) 250
BstXI CCANNNNNNTGG 1 cut(s) 184
BsuRI GGCC 3 cut(s) 57, 314, 356
BtgZI GCGATG 1 cut(s) 34
Cac8I GCNNGC 3 cut(s) 7, 55, 193
CfoI GCGC 4 cut(s) 7, 9, 32, 289
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 313, 375
Csp6I GTAC 2 cut(s) 70, 302
CviJI RGCY 8 cut(s) 57, 159, 188, 195, 314, 356, 399, 410
CviKI_1 RGCY 8 cut(s) 57, 159, 188, 195, 314, 356, 399, 410
CviQI GTAC 2 cut(s) 70, 302
DdeI CTNAG 2 cut(s) 222, 276
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 375
FaiI YATR 5 cut(s) 93, 111, 153, 198, 429
FaqI GGGAC 1 cut(s) 388
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 189, 408
Fsp4HI GCNGC 2 cut(s) 189, 408
FspBI CTAG 1 cut(s) 393
GlaI GCGC 4 cut(s) 6, 8, 31, 288
GluI GCNGC 2 cut(s) 189, 408
HaeIII GGCC 3 cut(s) 57, 314, 356
HapII CCGG 3 cut(s) 54, 164, 378
HhaI GCGC 4 cut(s) 7, 9, 32, 289
Hin6I GCGC 4 cut(s) 5, 7, 30, 287
HinP1I GCGC 4 cut(s) 5, 7, 30, 287
HinfI GANTC 3 cut(s) 293, 361, 381
HpaII CCGG 3 cut(s) 54, 164, 378
HphI GGTGA 2 cut(s) 148, 153
Hpy166II GTNNAC 2 cut(s) 250, 349
Hpy188I TCNGA 1 cut(s) 233
Hpy8I GTNNAC 2 cut(s) 250, 349
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 3 cut(s) 79, 184, 256
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 349
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 38, 197
HpyF3I CTNAG 2 cut(s) 222, 276
HpySE526I ACGT 1 cut(s) 300
HspAI GCGC 4 cut(s) 5, 7, 30, 287
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 3 cut(s) 103, 396, 404
LpnPI CCDG 8 cut(s) 67, 177, 177, 230, 318, 357, 391, 402
Lsp1109I GCAGC 2 cut(s) 175, 419
LweI GCATC 1 cut(s) 2
MaeI CTAG 1 cut(s) 393
MaeII ACGT 1 cut(s) 300
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 2 cut(s) 250, 409
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 1 cut(s) 324
MluNI TGGCCA 1 cut(s) 356
MnlI CCTC 1 cut(s) 164
Mox20I TGGCCA 1 cut(s) 356
MroNI GCCGGC 1 cut(s) 53
MscI TGGCCA 1 cut(s) 356
MslI CAYNNNNRTG 1 cut(s) 150
Msp20I TGGCCA 1 cut(s) 356
MspI CCGG 3 cut(s) 54, 164, 378
MspR9I CCNGG 1 cut(s) 165
MvnI CGCG 4 cut(s) 7, 30, 287, 289
MwoI GCNNNNNNNGC 3 cut(s) 29, 38, 197
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 165
NdeII GATC 1 cut(s) 271
NgoMIV GCCGGC 1 cut(s) 53
NlaIV GGNNCC 4 cut(s) 175, 208, 315, 376
NmeAIII GCCGAG 1 cut(s) 67
PauI GCGCGC 1 cut(s) 5
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 293, 361, 381
PkrI GCNGC 2 cut(s) 190, 409
Ppu21I YACGTR 1 cut(s) 301
PspN4I GGNNCC 4 cut(s) 175, 208, 315, 376
PspPI GGNCC 2 cut(s) 313, 375
PteI GCGCGC 1 cut(s) 5
RsaI GTAC 2 cut(s) 71, 303
RsaNI GTAC 2 cut(s) 70, 302
RseI CAYNNNNRTG 1 cut(s) 150
SatI GCNGC 2 cut(s) 189, 408
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 2 cut(s) 313, 375
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 351
SetI ASST 5 cut(s) 161, 175, 303, 307, 401
SfaNI GCATC 1 cut(s) 2
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 150
Sse9I AATT 1 cut(s) 324
SsiI CCGC 1 cut(s) 210
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 3 cut(s) 79, 184, 256
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 420
TasI AATT 1 cut(s) 324
TatI WGTACW 1 cut(s) 69
TfiI GAWTC 3 cut(s) 293, 361, 381
TseI GCWGC 2 cut(s) 188, 407
TspDTI ATGAA 3 cut(s) 37, 155, 168
VneI GTGCAC 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 375
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.