RLG00000016822

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003977
Physical Location & Seq
Reverse (-)
10333840 .. 10334996
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016822

Sequence Viewer

Length: 870 bp
ATGTTGAAGGAAGTGAGTGAAGAAGGTTTAGAGGTTCACGAAGAGCAGCAGCGTGAGAAGGACCATGTAGAGCCACCACAGGTTCTTCTCTTGGAGCTGGGAGAGCTTCAACGATGGTCGTTTTACAGAGCCGTGATAGCGGAGTTTGTCGCCACTCTTCTCTTCCTATATGCATGTCACGGCTGCCACCATCATCGGACACAAGAAGGAAGCTGCACAGTGCGATGGCGTTGGTGTTCTTGGCATTGCCTGGGCCTTTGGTGGCATGATCTTCGTCCTGGTTTACTCCATTGCTGGAATCTCAGTTCAGGCGGTCACATCAACCCCGCGGTGACTTTCGGGCTGTTGGTTGGGAGGAGGAGGGTGTCACTGCTCCGAACAGTGGCTTACATGGTTGCACAATGCTTAGGAGGCATATGCGGCGTAAGGTTAGTGAAAGCTTTGATGAAGCATTACTACCTCACGCATGGAGGTGGTGCCAACTCGGTTGCTCGTGATCATTCCAAAGGCGCTGCTTTGGGAGCTGAAATAGTCGGCACTTTTCTTCTGGTCTACACAGTCCTCTCTGCCACAGACGCTAAGAGAAGCGCACGTGACTCCCATGTGCCTGTATTGGTTCCATTGCCAATAGGGTTTGCTGTGTTTATGGTTCACCTGGCCACCATACCTATTACCGGGACTGGGATCAACCCTGCTAGGAGTTTTGCTGCTGCTGTAATCTACAACAATGACAAAGTCTGGGACGACCAGTGGATATTCTTGGTCGGACCATTCATTGGAGCTCTGACAGCAGCAGCTTATAACCAGTATGTACTGAGGGCATCAGCTAGTAAAGCCTTCAGATCGTTCCACAACAATACCACCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

290

Amino Acids

32.18

Weight (kDa)

8.79

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 35 - 266 1.9e-64 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 801
AccB1I GGYRCC 1 cut(s) 476
AccB7I CCANNNNNTGG 1 cut(s) 776
AccI GTMKAC 1 cut(s) 552
AccII CGCG 1 cut(s) 329
AciI CCGC 5 cut(s) 140, 312, 327, 329, 420
AclWI GGATC 1 cut(s) 692
AcoI YGGCCR 1 cut(s) 657
AcuI CTGAAG 1 cut(s) 823
AcvI CACGTG 1 cut(s) 593
AfaI GTAC 1 cut(s) 813
AfiI CCNNNNNNNGG 4 cut(s) 382, 674, 681, 776
AgsI TTSAA 2 cut(s) 7, 110
AjnI CCWGG 3 cut(s) 249, 277, 654
AloI GAACNNNNNNTCC 2 cut(s) 289, 321
AluBI AGCT 8 cut(s) 97, 106, 213, 440, 524, 782, 797, 827
AluI AGCT 8 cut(s) 97, 106, 213, 440, 524, 782, 797, 827
Alw21I GWGCWC 1 cut(s) 784
AlwI GGATC 1 cut(s) 692
AoxI GGCC 2 cut(s) 253, 657
ApeKI GCWGC 9 cut(s) 46, 49, 183, 213, 512, 707, 710, 791, 794
AspLEI GCGC 2 cut(s) 512, 590
AspS9I GGNCC 3 cut(s) 61, 253, 767
AsuC2I CCSGG 1 cut(s) 676
AsuHPI GGTGA 2 cut(s) 343, 644
AvaII GGWCC 2 cut(s) 61, 767
BalI TGGCCA 1 cut(s) 659
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 1 cut(s) 784
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BauI CACGAG 1 cut(s) 492
BbrPI CACGTG 1 cut(s) 593
Bbv12I GWGCWC 1 cut(s) 784
BbvI GCAGC 9 cut(s) 58, 61, 170, 200, 499, 694, 697, 803, 806
BccI CCATC 3 cut(s) 108, 198, 219
BceAI ACGGC 2 cut(s) 116, 196
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 3 cut(s) 251, 279, 656
BclI TGATCA 1 cut(s) 496
BcnI CCSGG 1 cut(s) 676
BfaI CTAG 2 cut(s) 696, 828
BfoI RGCGCY 1 cut(s) 513
Bme1390I CCNGG 4 cut(s) 251, 279, 656, 676
Bme18I GGWCC 2 cut(s) 61, 767
BmgT120I GGNCC 3 cut(s) 61, 253, 767
BmiI GGNNCC 2 cut(s) 478, 618
BmrFI CCNGG 4 cut(s) 251, 279, 656, 676
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 1 cut(s) 830
BmuI ACTGGG 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 593
BsaJI CCNNGG 2 cut(s) 250, 327
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 674, 681, 776
Bse1I ACTGG 3 cut(s) 685, 748, 805
Bse3DI GCAATG 3 cut(s) 244, 289, 620
BseBI CCWGG 3 cut(s) 251, 279, 656
BseDI CCNNGG 2 cut(s) 250, 327
BseLI CCNNNNNNNGG 4 cut(s) 382, 674, 681, 776
BseMI GCAATG 3 cut(s) 244, 289, 620
BseMII CTCAG 2 cut(s) 316, 806
BseNI ACTGG 3 cut(s) 685, 748, 805
BseRI GAGGAG 2 cut(s) 370, 373
BseXI GCAGC 9 cut(s) 58, 61, 170, 200, 499, 694, 697, 803, 806
BseYI CCCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 2 cut(s) 255, 659
BshNI GGYRCC 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 784
BsiSI CCGG 1 cut(s) 675
BslFI GGGAC 2 cut(s) 691, 755
BslI CCNNNNNNNGG 4 cut(s) 382, 674, 681, 776
BsmFI GGGAC 2 cut(s) 691, 755
BsnI GGCC 2 cut(s) 255, 659
Bsp1286I GDGCHC 1 cut(s) 784
Bsp143I GATC 4 cut(s) 268, 496, 684, 842
BspACI CCGC 5 cut(s) 140, 312, 327, 329, 420
BspANI GGCC 2 cut(s) 255, 659
BspCNI CTCAG 2 cut(s) 315, 807
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 2 cut(s) 478, 618
BspPI GGATC 1 cut(s) 692
BspQI GCTCTTC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 476
BsrDI GCAATG 3 cut(s) 244, 289, 620
BsrI ACTGG 3 cut(s) 685, 748, 805
BssECI CCNNGG 2 cut(s) 250, 327
BssMI GATC 4 cut(s) 268, 496, 684, 842
BssSI CACGAG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 492
Bst2UI CCWGG 3 cut(s) 251, 279, 656
Bst4CI ACNGT 3 cut(s) 220, 382, 559
Bst6I CTCTTC 3 cut(s) 36, 162, 167
BstBAI YACGTR 1 cut(s) 593
BstDEI CTNAG 4 cut(s) 302, 406, 579, 815
BstDSI CCRYGG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 329
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 2 cut(s) 512, 590
BstKTI GATC 4 cut(s) 271, 499, 687, 845
BstMBI GATC 4 cut(s) 268, 496, 684, 842
BstMWI GCNNNNNNNGC 8 cut(s) 103, 137, 411, 420, 521, 575, 788, 833
BstNI CCWGG 3 cut(s) 251, 279, 656
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 4 cut(s) 249, 277, 654, 674
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 9 cut(s) 58, 61, 170, 200, 499, 694, 697, 803, 806
BsuRI GGCC 2 cut(s) 255, 659
BtgI CCRYGG 1 cut(s) 327
BtgZI GCGATG 1 cut(s) 238
BtsI GCAGTG 1 cut(s) 368
BtsIMutI CAGTG 4 cut(s) 225, 368, 387, 755
CfoI GCGC 2 cut(s) 512, 590
Cfr13I GGNCC 3 cut(s) 61, 253, 767
Cfr42I CCGCGG 1 cut(s) 330
CseI GACGC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 812
CviAII CATG 6 cut(s) 65, 174, 266, 391, 467, 602
CviQI GTAC 1 cut(s) 812
DdeI CTNAG 4 cut(s) 302, 406, 579, 815
DpnI GATC 4 cut(s) 270, 498, 686, 844
DpnII GATC 4 cut(s) 268, 496, 684, 842
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 3 cut(s) 36, 162, 167
EarI CTCTTC 3 cut(s) 36, 162, 167
Ecl136II GAGCTC 1 cut(s) 782
Eco24I GRGCYC 1 cut(s) 784
Eco47I GGWCC 2 cut(s) 61, 767
Eco53kI GAGCTC 1 cut(s) 782
Eco57I CTGAAG 1 cut(s) 823
Eco72I CACGTG 1 cut(s) 593
EcoICRI GAGCTC 1 cut(s) 782
EcoRII CCWGG 3 cut(s) 249, 277, 654
EcoT22I ATGCAT 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 784
FaeI CATG 6 cut(s) 68, 177, 269, 394, 470, 605
FaqI GGGAC 2 cut(s) 691, 755
FatI CATG 6 cut(s) 64, 173, 265, 390, 466, 601
FauI CCCGC 1 cut(s) 334
FauNDI CATATG 1 cut(s) 416
FbaI TGATCA 1 cut(s) 496
FblI GTMKAC 1 cut(s) 552
FriOI GRGCYC 1 cut(s) 784
FspBI CTAG 2 cut(s) 696, 828
GlaI GCGC 2 cut(s) 511, 589
GsaI CCCAGC 1 cut(s) 101
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 2 cut(s) 255, 659
HapII CCGG 1 cut(s) 675
HgaI GACGC 1 cut(s) 584
HhaI GCGC 2 cut(s) 512, 590
Hin1II CATG 6 cut(s) 68, 177, 269, 394, 470, 605
Hin6I GCGC 2 cut(s) 510, 588
HinP1I GCGC 2 cut(s) 510, 588
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 2 cut(s) 298, 596
HpaII CCGG 1 cut(s) 675
HphI GGTGA 2 cut(s) 343, 644
Hpy166II GTNNAC 4 cut(s) 37, 284, 553, 652
Hpy188I TCNGA 5 cut(s) 198, 377, 767, 786, 842
Hpy188III TCNNGA 2 cut(s) 38, 494
Hpy8I GTNNAC 4 cut(s) 37, 284, 553, 652
HpyAV CCTTC 4 cut(s) 17, 52, 200, 847
HpyCH4III ACNGT 3 cut(s) 220, 382, 559
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 3 cut(s) 173, 216, 398
HpyF10VI GCNNNNNNNGC 8 cut(s) 103, 137, 411, 420, 521, 575, 788, 833
HpyF3I CTNAG 4 cut(s) 302, 406, 579, 815
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 6 cut(s) 68, 177, 269, 394, 470, 605
HspAI GCGC 2 cut(s) 510, 588
Ksp22I TGATCA 1 cut(s) 496
KspI CCGCGG 1 cut(s) 330
Kzo9I GATC 4 cut(s) 268, 496, 684, 842
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 94, 378, 521, 779
Lsp1109I GCAGC 9 cut(s) 58, 61, 170, 200, 499, 694, 697, 803, 806
LweI GCATC 1 cut(s) 830
MaeI CTAG 2 cut(s) 696, 828
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 5 cut(s) 176, 314, 331, 366, 593
MalI GATC 4 cut(s) 270, 498, 686, 844
MboI GATC 4 cut(s) 268, 496, 684, 842
MboII GAAGA 7 cut(s) 32, 53, 77, 149, 154, 263, 536
MhlI GDGCHC 1 cut(s) 784
MlsI TGGCCA 1 cut(s) 659
MluNI TGGCCA 1 cut(s) 659
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 1 cut(s) 745
MnlI CCTC 9 cut(s) 25, 348, 351, 354, 404, 464, 470, 572, 810
Mox20I TGGCCA 1 cut(s) 659
Mph1103I ATGCAT 1 cut(s) 175
MscI TGGCCA 1 cut(s) 659
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 659
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 1 cut(s) 675
MspR9I CCNGG 4 cut(s) 251, 279, 656, 676
MvaI CCWGG 3 cut(s) 251, 279, 656
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 8 cut(s) 103, 137, 411, 420, 521, 575, 788, 833
NciI CCSGG 1 cut(s) 676
NdeI CATATG 1 cut(s) 416
NdeII GATC 4 cut(s) 268, 496, 684, 842
NlaIII CATG 6 cut(s) 68, 177, 269, 394, 470, 605
NlaIV GGNNCC 2 cut(s) 478, 618
NmuCI GTSAC 5 cut(s) 176, 314, 331, 366, 593
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 1 cut(s) 177
PciSI GCTCTTC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 298
PflFI GACNNNGTC 1 cut(s) 734
PflMI CCANNNNNTGG 1 cut(s) 776
PleI GAGTC 1 cut(s) 590
PmaCI CACGTG 1 cut(s) 593
PmlI CACGTG 1 cut(s) 593
PpsI GAGTC 1 cut(s) 590
Ppu21I YACGTR 1 cut(s) 593
PsiI TTATAA 1 cut(s) 801
Psp124BI GAGCTC 1 cut(s) 784
Psp6I CCWGG 3 cut(s) 249, 277, 654
PspCI CACGTG 1 cut(s) 593
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 3 cut(s) 249, 277, 654
PspN4I GGNNCC 2 cut(s) 478, 618
PspPI GGNCC 3 cut(s) 61, 253, 767
PsyI GACNNNGTC 1 cut(s) 734
RsaI GTAC 1 cut(s) 813
RsaNI GTAC 1 cut(s) 812
RseI CAYNNNNRTG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 784
SacII CCGCGG 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 36
Sau3AI GATC 4 cut(s) 268, 496, 684, 842
Sau96I GGNCC 3 cut(s) 61, 253, 767
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 4 cut(s) 251, 279, 656, 676
SduI GDGCHC 1 cut(s) 784
SfaNI GCATC 1 cut(s) 830
Sfr303I CCGCGG 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 330
SinI GGWCC 2 cut(s) 61, 767
SmiMI CAYNNNNRTG 1 cut(s) 471
SsiI CCGC 5 cut(s) 140, 312, 327, 329, 420
SspMI CTAG 2 cut(s) 696, 828
SstI GAGCTC 1 cut(s) 784
StyD4I CCNGG 4 cut(s) 249, 277, 654, 674
TaaI ACNGT 3 cut(s) 220, 382, 559
TaiI ACGT 1 cut(s) 595
TatI WGTACW 1 cut(s) 811
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 1 cut(s) 298
TscAI CASTG 4 cut(s) 225, 375, 387, 755
TseFI GTSAC 5 cut(s) 176, 314, 331, 366, 593
TseI GCWGC 9 cut(s) 46, 49, 183, 213, 512, 707, 710, 791, 794
Tsp45I GTSAC 5 cut(s) 176, 314, 331, 366, 593
TspDTI ATGAA 2 cut(s) 461, 763
TspRI CASTG 4 cut(s) 225, 375, 387, 755
Tth111I GACNNNGTC 1 cut(s) 734
Van91I CCANNNNNTGG 1 cut(s) 776
VpaK11BI GGWCC 2 cut(s) 61, 767
XceI RCATGY 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 2 cut(s) 696, 828
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.