FvH4_1g11250

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003977
Physical Location & Seq
Reverse (-)
6133902 .. 6135270
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11250.t1

Sequence Viewer

Length: 855 bp
ATGTTGAAGGAAGTGAGTGAAGAAGGTTTGAAGGTTCATGAAGAGCAGCATGGGAAGGACTACGTAGAGCCACCACCGGTTCCGCTCTTGGAGCTGGGAGAGCTTCAGCGTTGGTCGTTTTACAGAGCTGTGATAGCAGAGTTTGTTGCCACTCTTCTCTTCCTCTATGTCACGGTTGCCACCGTCATCGGACACAAGAAGGAAGCAGTTCAGTGTGATGGTGTTGGTGTTCTTGGCATTGCCTGGGCCTTCGGTGGCATGATCTTCGTCCTCGTTTACTCTACAGCTGGAATATCAGGTGGTCACATCAATCCGGCGGTGACTTTCGGGCTGTTGGTTGCGAGGAGGGTGTCGCTGCTCAGAGCAGTGGCTTACACGGTTGCGCAATGCTTGGGAGGCATATGCGGCGTAGGGTTAGTGAAAGCTTTGATGAAACATTACTACCTCACGTATGGAGGTGGTGCCAACTCGGTTGCTCCTGATTATACCAAAGGCGCTGCTTTAGGAGCTGAAACAGTTGGCACTTTTCTTCTGGTATACACCGTCTTCTCTGCCACAGATGCCAAGAGAAGCGCACGCGACTCCCATGTGCCTGTATTGGCTCCATTGCCAATAGGGTTTGCTGTGTTTATGGTTCACCTGGCCACCATTCCTATTACTGGCACTGGGATCAACCCTGCTAGGAGCTTTGCTGCTGCGGTGGTCTACAACAATGACAAAGTCTGGGACGACCAGTGGATATTCTGGGTTGGACCATTCCTGGGAGCTCTGATAGCAGCAGCTTATAACCAGCATGTACTGCGGGCATCAAGCAGTAAAGCCTTTGGTTCGTTCTACATCAACACCACGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

285

Amino Acids

30.42

Weight (kDa)

7.75

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 34 - 261 1e-78 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
Acc16I TGCGCA 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 461
AccBSI CCGCTC 1 cut(s) 85
AccI GTMKAC 2 cut(s) 537, 705
AccII CGCG 1 cut(s) 579
AciI CCGC 5 cut(s) 83, 317, 405, 698, 802
AclWI GGATC 1 cut(s) 677
AcoI YGGCCR 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 1 cut(s) 798
AfiI CCNNNNNNNGG 2 cut(s) 659, 761
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 2 cut(s) 7, 31
AjnI CCWGG 3 cut(s) 242, 639, 759
AluBI AGCT 9 cut(s) 94, 103, 128, 287, 425, 509, 687, 767, 782
AluI AGCT 9 cut(s) 94, 103, 128, 287, 425, 509, 687, 767, 782
Alw21I GWGCWC 1 cut(s) 769
AlwI GGATC 1 cut(s) 677
AoxI GGCC 2 cut(s) 246, 642
ApeKI GCWGC 7 cut(s) 46, 355, 497, 692, 695, 776, 779
AsiGI ACCGGT 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 3 cut(s) 385, 497, 575
AspS9I GGNCC 2 cut(s) 246, 752
AsuHPI GGTGA 2 cut(s) 331, 629
AvaII GGWCC 1 cut(s) 752
BalI TGGCCA 1 cut(s) 644
BanI GGYRCC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 769
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 7 cut(s) 58, 342, 484, 679, 682, 788, 791
BccI CCATC 1 cut(s) 212
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 3 cut(s) 244, 641, 761
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 282
BfoI RGCGCY 1 cut(s) 498
BisI GCNGC 8 cut(s) 47, 356, 406, 498, 693, 696, 777, 780
BlsI GCNGC 8 cut(s) 48, 357, 407, 499, 694, 697, 778, 781
Bme1390I CCNGG 3 cut(s) 244, 641, 761
Bme18I GGWCC 1 cut(s) 752
BmgT120I GGNCC 2 cut(s) 246, 752
BmiI GGNNCC 3 cut(s) 81, 463, 603
BmrFI CCNGG 3 cut(s) 244, 641, 761
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 2 cut(s) 550, 815
BmuI ACTGGG 1 cut(s) 675
BpiI GAAGAC 1 cut(s) 538
BsaAI YACGTR 2 cut(s) 64, 450
BsaJI CCNNGG 2 cut(s) 243, 760
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 2 cut(s) 659, 761
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 3 cut(s) 664, 670, 733
Bse3DI GCAATG 3 cut(s) 237, 392, 605
BseBI CCWGG 3 cut(s) 244, 641, 761
BseDI CCNNGG 2 cut(s) 243, 760
BseLI CCNNNNNNNGG 2 cut(s) 659, 761
BseMI GCAATG 3 cut(s) 237, 392, 605
BseMII CTCAG 1 cut(s) 373
BseNI ACTGG 3 cut(s) 664, 670, 733
BseRI GAGGAG 1 cut(s) 358
BseXI GCAGC 7 cut(s) 58, 342, 484, 679, 682, 788, 791
BseYI CCCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 2 cut(s) 248, 644
BshNI GGYRCC 1 cut(s) 461
BshTI ACCGGT 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 769
BsiSI CCGG 2 cut(s) 77, 314
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 2 cut(s) 659, 761
BsmFI GGGAC 1 cut(s) 740
BsnI GGCC 2 cut(s) 248, 644
Bsp1286I GDGCHC 1 cut(s) 769
Bsp143I GATC 2 cut(s) 261, 669
BspACI CCGC 5 cut(s) 83, 317, 405, 698, 802
BspANI GGCC 2 cut(s) 248, 644
BspCNI CTCAG 1 cut(s) 372
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 37
BspLI GGNNCC 3 cut(s) 81, 463, 603
BspPI GGATC 1 cut(s) 677
BspQI GCTCTTC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 3 cut(s) 237, 392, 605
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 3 cut(s) 664, 670, 733
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 243, 760
BssMI GATC 2 cut(s) 261, 669
BssNAI GTATAC 1 cut(s) 538
Bst1107I GTATAC 1 cut(s) 538
Bst2UI CCWGG 3 cut(s) 244, 641, 761
Bst4CI ACNGT 5 cut(s) 175, 184, 379, 517, 544
Bst6I CTCTTC 3 cut(s) 36, 159, 164
BstAPI GCANNNNNTGC 1 cut(s) 799
BstBAI YACGTR 2 cut(s) 64, 450
BstC8I GCNNGC 2 cut(s) 577, 804
BstDEI CTNAG 1 cut(s) 359
BstFNI CGCG 1 cut(s) 579
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 3 cut(s) 385, 497, 575
BstKTI GATC 2 cut(s) 264, 672
BstMBI GATC 2 cut(s) 261, 669
BstMWI GCNNNNNNNGC 9 cut(s) 91, 100, 134, 396, 405, 506, 560, 773, 799
BstNI CCWGG 3 cut(s) 244, 641, 761
BstNSI RCATGY 1 cut(s) 797
BstSCI CCNGG 3 cut(s) 242, 639, 759
BstSFI CTRYAG 1 cut(s) 282
BstSNI TACGTA 1 cut(s) 64
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 7 cut(s) 58, 342, 484, 679, 682, 788, 791
BstV2I GAAGAC 1 cut(s) 538
BstZ17I GTATAC 1 cut(s) 538
BsuRI GGCC 2 cut(s) 248, 644
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 4 cut(s) 218, 372, 663, 740
Cac8I GCNNGC 2 cut(s) 577, 804
CciI TCATGA 1 cut(s) 37
CfoI GCGC 3 cut(s) 385, 497, 575
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 246, 752
Csp6I GTAC 1 cut(s) 797
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 5 cut(s) 38, 50, 259, 587, 794
CviQI GTAC 1 cut(s) 797
DdeI CTNAG 1 cut(s) 359
DpnI GATC 2 cut(s) 263, 671
DpnII GATC 2 cut(s) 261, 669
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 3 cut(s) 36, 159, 164
EarI CTCTTC 3 cut(s) 36, 159, 164
Ecl136II GAGCTC 1 cut(s) 767
Eco105I TACGTA 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 769
Eco47I GGWCC 1 cut(s) 752
Eco53kI GAGCTC 1 cut(s) 767
Eco57I CTGAAG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 767
EcoRII CCWGG 3 cut(s) 242, 639, 759
EcoT38I GRGCYC 1 cut(s) 769
FaeI CATG 5 cut(s) 41, 53, 262, 590, 797
FaqI GGGAC 1 cut(s) 740
FatI CATG 5 cut(s) 37, 49, 258, 586, 793
FauI CCCGC 1 cut(s) 795
FauNDI CATATG 1 cut(s) 401
FblI GTMKAC 2 cut(s) 537, 705
Fnu4HI GCNGC 8 cut(s) 47, 356, 406, 498, 693, 696, 777, 780
FriOI GRGCYC 1 cut(s) 769
Fsp4HI GCNGC 8 cut(s) 47, 356, 406, 498, 693, 696, 777, 780
FspBI CTAG 1 cut(s) 681
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 3 cut(s) 384, 496, 574
GluI GCNGC 8 cut(s) 47, 356, 406, 498, 693, 696, 777, 780
GsaI CCCAGC 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 2 cut(s) 248, 644
HapII CCGG 2 cut(s) 77, 314
HhaI GCGC 3 cut(s) 385, 497, 575
Hin1II CATG 5 cut(s) 41, 53, 262, 590, 797
Hin6I GCGC 3 cut(s) 383, 495, 573
HinP1I GCGC 3 cut(s) 383, 495, 573
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 1 cut(s) 581
HpaII CCGG 2 cut(s) 77, 314
HphI GGTGA 2 cut(s) 331, 629
Hpy166II GTNNAC 4 cut(s) 277, 538, 637, 706
Hpy188I TCNGA 3 cut(s) 191, 362, 771
Hpy188III TCNNGA 2 cut(s) 38, 479
Hpy8I GTNNAC 4 cut(s) 277, 538, 637, 706
HpyAV CCTTC 5 cut(s) 17, 25, 49, 193, 259
HpyCH4III ACNGT 5 cut(s) 175, 184, 379, 517, 544
HpyCH4IV ACGT 2 cut(s) 63, 449
HpyF10VI GCNNNNNNNGC 9 cut(s) 91, 100, 134, 396, 405, 506, 560, 773, 799
HpyF3I CTNAG 1 cut(s) 359
HpySE526I ACGT 2 cut(s) 63, 449
Hsp92II CATG 5 cut(s) 41, 53, 262, 590, 797
HspAI GCGC 3 cut(s) 383, 495, 573
Kzo9I GATC 2 cut(s) 261, 669
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 6 cut(s) 91, 481, 506, 607, 684, 764
Lsp1109I GCAGC 7 cut(s) 58, 342, 484, 679, 682, 788, 791
LweI GCATC 2 cut(s) 550, 815
MaeI CTAG 1 cut(s) 681
MaeII ACGT 2 cut(s) 63, 449
MaeIII GTNAC 3 cut(s) 169, 302, 319
MalI GATC 2 cut(s) 263, 671
MbiI CCGCTC 1 cut(s) 85
MboI GATC 2 cut(s) 261, 669
MboII GAAGA 7 cut(s) 32, 53, 146, 151, 256, 521, 538
MhlI GDGCHC 1 cut(s) 769
MlsI TGGCCA 1 cut(s) 644
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 730
MnlI CCTC 7 cut(s) 173, 281, 336, 339, 389, 449, 455
Mox20I TGGCCA 1 cut(s) 644
MroXI GAANNNNTTC 1 cut(s) 207
MscI TGGCCA 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 2 cut(s) 77, 314
MspR9I CCNGG 3 cut(s) 244, 641, 761
MvaI CCWGG 3 cut(s) 244, 641, 761
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 9 cut(s) 91, 100, 134, 396, 405, 506, 560, 773, 799
NdeI CATATG 1 cut(s) 401
NdeII GATC 2 cut(s) 261, 669
NlaIII CATG 5 cut(s) 41, 53, 262, 590, 797
NlaIV GGNNCC 3 cut(s) 81, 463, 603
NmuCI GTSAC 3 cut(s) 169, 302, 319
NsbI TGCGCA 1 cut(s) 384
NspI RCATGY 1 cut(s) 797
PagI TCATGA 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 207
PflFI GACNNNGTC 1 cut(s) 719
PinAI ACCGGT 1 cut(s) 76
PkrI GCNGC 8 cut(s) 48, 357, 407, 499, 694, 697, 778, 781
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
Ppu21I YACGTR 2 cut(s) 64, 450
PsiI TTATAA 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 769
Psp6I CCWGG 3 cut(s) 242, 639, 759
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 3 cut(s) 242, 639, 759
PspN4I GGNNCC 3 cut(s) 81, 463, 603
PspPI GGNCC 2 cut(s) 246, 752
PsyI GACNNNGTC 1 cut(s) 719
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
SacI GAGCTC 1 cut(s) 769
SapI GCTCTTC 1 cut(s) 36
SatI GCNGC 8 cut(s) 47, 356, 406, 498, 693, 696, 777, 780
Sau3AI GATC 2 cut(s) 261, 669
Sau96I GGNCC 2 cut(s) 246, 752
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 3 cut(s) 244, 641, 761
SduI GDGCHC 1 cut(s) 769
SfaNI GCATC 2 cut(s) 550, 815
SfcI CTRYAG 1 cut(s) 282
SinI GGWCC 1 cut(s) 752
SnaBI TACGTA 1 cut(s) 64
SsiI CCGC 5 cut(s) 83, 317, 405, 698, 802
SspMI CTAG 1 cut(s) 681
SstI GAGCTC 1 cut(s) 769
StyD4I CCNGG 3 cut(s) 242, 639, 759
TaaI ACNGT 5 cut(s) 175, 184, 379, 517, 544
TaiI ACGT 2 cut(s) 66, 452
TatI WGTACW 1 cut(s) 796
TauI GCSGC 1 cut(s) 408
TscAI CASTG 4 cut(s) 218, 372, 670, 740
TseFI GTSAC 3 cut(s) 169, 302, 319
TseI GCWGC 7 cut(s) 46, 355, 497, 692, 695, 776, 779
Tsp45I GTSAC 3 cut(s) 169, 302, 319
TspDTI ATGAA 3 cut(s) 26, 54, 446
TspRI CASTG 4 cut(s) 218, 372, 670, 740
Tth111I GACNNNGTC 1 cut(s) 719
VpaK11BI GGWCC 1 cut(s) 752
XceI RCATGY 1 cut(s) 797
XmiI GTMKAC 2 cut(s) 537, 705
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.