MD05G1122600.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003977
Physical Location & Seq
Forward (+)
24537335 .. 24538852
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1122600.v1.1.491

Sequence Viewer

Length: 846 bp
ATGTCCAAGGAAGTGAGCGAGGAGGGACGAGCCCACCACCATGGGAAGGACTACACCGACCCACCGCCAGCTCCCCTCATCGACTTCGACGAGCTCAGGCGGTGGTCCTTCTACAGGGCCCTCATCGCCGAGTTCGTCGCCACCCTACTCTTCCTCTACATCACCGTAGCCACCGTCATCGGCCACAAGAACTCACTCGGGGACTGCGGCGGCGTTGGCCTTCTTGGCATCGCCTGGGCCTTCGGCGGCATGATCTTCGTACTTGTCTACTGCACCGCCGGAATCTCTGGTGGTCATATTAACCCAGCTGTGACTTTTGGCCTTTTCTTGGCCCGCAAGGTCTCGCTCATCAGAGCCGTGGCTTACATGGTGGCCCAGTGCTTGGGAGCTATCGTCGGCGTTGGGCTGGTCAAGGCCTTCCAGAAGCACAAGTACAACTCCGTCGGCGGCGGCGCCAACTCTGTGGCTAGTGGCTACAGCAAGGGCACAGCTCTGGGAGCTGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCGGCCACCGACCCCAAGAGGAGCGCACGCGACTCCCACGTGCCTGTTTTGGCTCCACTGCCCATTGGGTTTGCTGTGTTTATTGTGCACCTCGCAACAATCCCCATTACTGGAACTGGAATCAACCCAGCAAGGAGTTTCGGCGCTGCTGTGATCTACAACAACGATAAAATCTGGGATGACCACTGGATCTTCTGGGTCGGACCATTTGTGGGAGCACTGGCTGCGGCGGCGTACCACCAGTACATTCTGAGGGCGGCGGCGCTCAAGGCGTTGGGATCCTTCCGCAGCAACCCAACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

282

Amino Acids

29.92

Weight (kDa)

9.22

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 260 3.2e-84 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 452, 512
AccB7I CCANNNNNTGG 1 cut(s) 382
AccI GTMKAC 2 cut(s) 267, 528
AccII CGCG 1 cut(s) 570
AclWI GGATC 3 cut(s) 737, 813, 826
AcoI YGGCCR 2 cut(s) 181, 543
AcvI CACGTG 1 cut(s) 580
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 4 cut(s) 261, 434, 776, 785
AfiI CCNNNNNNNGG 6 cut(s) 46, 114, 328, 382, 650, 752
AjnI CCWGG 1 cut(s) 233
AluBI AGCT 6 cut(s) 71, 94, 308, 389, 491, 500
AluI AGCT 6 cut(s) 71, 94, 308, 389, 491, 500
Alw21I GWGCWC 3 cut(s) 96, 630, 760
Alw26I GTCTC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 626
AlwI GGATC 3 cut(s) 737, 813, 826
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 9 cut(s) 117, 181, 217, 237, 319, 330, 372, 414, 543
ApaI GGGCCC 1 cut(s) 121
ApaLI GTGCAC 1 cut(s) 626
ApeKI GCWGC 3 cut(s) 686, 764, 828
AspLEI GCGC 4 cut(s) 455, 566, 686, 805
AspS9I GGNCC 7 cut(s) 105, 117, 118, 237, 331, 373, 743
AsuHPI GGTGA 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 2 cut(s) 105, 743
BaeGI GKGCMC 3 cut(s) 121, 488, 630
BamHI GGATCC 1 cut(s) 818
BanI GGYRCC 2 cut(s) 452, 512
BanII GRGCYC 3 cut(s) 34, 96, 121
BarI GAAGNNNNNNTAC 2 cut(s) 416, 448
BbrPI CACGTG 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 3 cut(s) 96, 630, 760
BbvI GCAGC 3 cut(s) 673, 751, 840
BceAI ACGGC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciT130I CCWGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 1 cut(s) 468
BfmI CTRYAG 2 cut(s) 112, 475
BfoI RGCGCY 3 cut(s) 456, 687, 806
BglI GCCNNNNNGGC 1 cut(s) 225
Bme1390I CCNGG 1 cut(s) 235
Bme18I GGWCC 2 cut(s) 105, 743
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 7 cut(s) 105, 117, 118, 237, 331, 373, 743
BmiI GGNNCC 5 cut(s) 119, 454, 514, 594, 820
BmrFI CCNGG 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 370
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 529
Bpu10I CCTNAGC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 791
BsaAI YACGTR 1 cut(s) 580
BsaHI GRCGYC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 4 cut(s) 6, 40, 234, 357
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 114, 328, 382, 650, 752
Bse1I ACTGG 6 cut(s) 376, 655, 661, 731, 765, 781
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 4 cut(s) 6, 40, 234, 357
BseGI GGATG 1 cut(s) 724
BseLI CCNNNNNNNGG 6 cut(s) 46, 114, 328, 382, 650, 752
BseMII CTCAG 3 cut(s) 109, 492, 782
BseNI ACTGG 6 cut(s) 376, 655, 661, 731, 765, 781
BseRI GAGGAG 2 cut(s) 35, 574
BseSI GKGCMC 3 cut(s) 121, 488, 630
BseXI GCAGC 3 cut(s) 673, 751, 840
BseYI CCCAGC 2 cut(s) 304, 667
BsgI GTGCAG 1 cut(s) 256
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 9 cut(s) 119, 183, 219, 239, 321, 332, 374, 416, 545
BshNI GGYRCC 2 cut(s) 452, 512
BsiHKAI GWGCWC 3 cut(s) 96, 630, 760
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 279
BslFI GGGAC 2 cut(s) 39, 215
BslI CCNNNNNNNGG 6 cut(s) 46, 114, 328, 382, 650, 752
BsmAI GTCTC 1 cut(s) 346
BsmFI GGGAC 2 cut(s) 39, 215
BsnI GGCC 9 cut(s) 119, 183, 219, 239, 321, 332, 374, 416, 545
Bso31I GGTCTC 1 cut(s) 346
BsoBI CYCGRG 1 cut(s) 197
Bsp120I GGGCCC 1 cut(s) 117
Bsp1286I GDGCHC 6 cut(s) 34, 96, 121, 488, 630, 760
Bsp143I GATC 5 cut(s) 252, 504, 693, 729, 818
Bsp19I CCATGG 1 cut(s) 40
BspANI GGCC 9 cut(s) 119, 183, 219, 239, 321, 332, 374, 416, 545
BspCNI CTCAG 3 cut(s) 108, 493, 783
BspFNI CGCG 1 cut(s) 570
BspLI GGNNCC 5 cut(s) 119, 454, 514, 594, 820
BspPI GGATC 3 cut(s) 737, 813, 826
BspT107I GGYRCC 2 cut(s) 452, 512
BspTNI GGTCTC 1 cut(s) 346
BsrI ACTGG 6 cut(s) 376, 655, 661, 731, 765, 781
BssECI CCNNGG 4 cut(s) 6, 40, 234, 357
BssMI GATC 5 cut(s) 252, 504, 693, 729, 818
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 2 cut(s) 6, 40
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 3 cut(s) 166, 175, 535
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 453
BstAPI GCANNNNNTGC 1 cut(s) 764
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 69, 334, 568
BstDEI CTNAG 3 cut(s) 95, 501, 791
BstDSI CCRYGG 2 cut(s) 40, 357
BstENI CCTNNNNNAGG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 724
BstFNI CGCG 1 cut(s) 570
BstH2I RGCGCY 3 cut(s) 456, 687, 806
BstHHI GCGC 4 cut(s) 455, 566, 686, 805
BstKTI GATC 5 cut(s) 255, 507, 696, 732, 821
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 5 cut(s) 252, 504, 693, 729, 818
BstMWI GCNNNNNNNGC 7 cut(s) 125, 216, 225, 497, 764, 770, 809
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 233
BstSFI CTRYAG 2 cut(s) 112, 475
BstSLI GKGCMC 3 cut(s) 121, 488, 630
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 3 cut(s) 673, 751, 840
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 2 cut(s) 729, 818
BstXI CCANNNNNNTGG 2 cut(s) 41, 463
BstYI RGATCY 2 cut(s) 729, 818
BsuRI GGCC 9 cut(s) 119, 183, 219, 239, 321, 332, 374, 416, 545
BtgI CCRYGG 2 cut(s) 40, 357
BtgZI GCGATG 2 cut(s) 109, 214
BtsCI GGATG 1 cut(s) 724
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 4 cut(s) 383, 596, 724, 758
Cac8I GCNNGC 3 cut(s) 69, 334, 568
CfoI GCGC 4 cut(s) 455, 566, 686, 805
Cfr13I GGNCC 7 cut(s) 105, 117, 118, 237, 331, 373, 743
Csp6I GTAC 4 cut(s) 260, 433, 775, 784
CviAII CATG 3 cut(s) 41, 250, 367
CviQI GTAC 4 cut(s) 260, 433, 775, 784
DdeI CTNAG 3 cut(s) 95, 501, 791
DinI GGCGCC 1 cut(s) 454
DpnI GATC 5 cut(s) 254, 506, 695, 731, 820
DpnII GATC 5 cut(s) 252, 504, 693, 729, 818
EaeI YGGCCR 2 cut(s) 181, 543
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 6, 40
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 3 cut(s) 34, 96, 121
Eco31I GGTCTC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 105, 743
Eco53kI GAGCTC 1 cut(s) 94
Eco72I CACGTG 1 cut(s) 580
Eco88I CYCGRG 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 112
EcoO109I RGGNCCY 2 cut(s) 117, 118
EcoRII CCWGG 1 cut(s) 233
EcoT14I CCWWGG 2 cut(s) 6, 40
EcoT38I GRGCYC 3 cut(s) 34, 96, 121
EgeI GGCGCC 1 cut(s) 454
EheI GGCGCC 1 cut(s) 454
ErhI CCWWGG 2 cut(s) 6, 40
FaeI CATG 3 cut(s) 44, 253, 370
FaiI YATR 4 cut(s) 42, 251, 297, 368
FaqI GGGAC 2 cut(s) 39, 215
FatI CATG 3 cut(s) 40, 249, 366
FauI CCCGC 1 cut(s) 341
FblI GTMKAC 2 cut(s) 267, 528
FokI GGATG 1 cut(s) 731
FriOI GRGCYC 3 cut(s) 34, 96, 121
FspBI CTAG 1 cut(s) 468
GlaI GCGC 4 cut(s) 454, 565, 685, 804
GsaI CCCAGC 2 cut(s) 308, 671
HaeII RGCGCY 3 cut(s) 456, 687, 806
HaeIII GGCC 9 cut(s) 119, 183, 219, 239, 321, 332, 374, 416, 545
HapII CCGG 1 cut(s) 279
HhaI GCGC 4 cut(s) 455, 566, 686, 805
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 3 cut(s) 44, 253, 370
Hin6I GCGC 4 cut(s) 453, 564, 684, 803
HinP1I GCGC 4 cut(s) 453, 564, 684, 803
HinfI GANTC 3 cut(s) 282, 572, 660
HpaII CCGG 1 cut(s) 279
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 3 cut(s) 268, 529, 628
Hpy188I TCNGA 3 cut(s) 353, 743, 792
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 3 cut(s) 268, 529, 628
Hpy99I CGWCG 4 cut(s) 92, 140, 398, 446
HpyAV CCTTC 7 cut(s) 40, 118, 230, 250, 427, 526, 832
HpyCH4III ACNGT 3 cut(s) 166, 175, 535
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 2 cut(s) 273, 628
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 216, 225, 497, 764, 770, 809
HpyF3I CTNAG 3 cut(s) 95, 501, 791
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 3 cut(s) 44, 253, 370
HspAI GCGC 4 cut(s) 453, 564, 684, 803
KasI GGCGCC 1 cut(s) 452
Kzo9I GATC 5 cut(s) 252, 504, 693, 729, 818
LmnI GCTCC 6 cut(s) 76, 386, 497, 561, 598, 755
Lsp1109I GCAGC 3 cut(s) 673, 751, 840
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 468
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 310
MalI GATC 5 cut(s) 254, 506, 695, 731, 820
MboI GATC 5 cut(s) 252, 504, 693, 729, 818
MboII GAAGA 4 cut(s) 142, 247, 529, 724
MflI RGATCY 2 cut(s) 729, 818
MhlI GDGCHC 6 cut(s) 34, 96, 121, 488, 630, 760
Mly113I GGCGCC 1 cut(s) 453
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 721
MnlI CCTC 9 cut(s) 13, 16, 86, 131, 164, 533, 552, 641, 786
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 1 cut(s) 235
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 7 cut(s) 125, 216, 225, 497, 764, 770, 809
NarI GGCGCC 1 cut(s) 453
NcoI CCATGG 1 cut(s) 40
NdeII GATC 5 cut(s) 252, 504, 693, 729, 818
NlaIII CATG 3 cut(s) 44, 253, 370
NlaIV GGNNCC 5 cut(s) 119, 454, 514, 594, 820
NmeAIII GCCGAG 2 cut(s) 154, 521
NmuCI GTSAC 1 cut(s) 310
PceI AGGCCT 1 cut(s) 416
PcsI WCGNNNNNNNCGW 4 cut(s) 87, 132, 516, 531
PfeI GAWTC 2 cut(s) 282, 660
PflMI CCANNNNNTGG 1 cut(s) 382
PleI GAGTC 1 cut(s) 566
PluTI GGCGCC 1 cut(s) 456
PmaCI CACGTG 1 cut(s) 580
PmlI CACGTG 1 cut(s) 580
PpsI GAGTC 1 cut(s) 566
Ppu21I YACGTR 1 cut(s) 580
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 233
PspCI CACGTG 1 cut(s) 580
PspFI CCCAGC 2 cut(s) 304, 667
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 5 cut(s) 119, 454, 514, 594, 820
PspOMI GGGCCC 1 cut(s) 117
PspPI GGNCC 7 cut(s) 105, 117, 118, 237, 331, 373, 743
PsuI RGATCY 2 cut(s) 729, 818
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 4 cut(s) 261, 434, 776, 785
RsaNI GTAC 4 cut(s) 260, 433, 775, 784
RseI CAYNNNNRTG 1 cut(s) 39
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 300
Sau3AI GATC 5 cut(s) 252, 504, 693, 729, 818
Sau96I GGNCC 7 cut(s) 105, 117, 118, 237, 331, 373, 743
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 1 cut(s) 235
SduI GDGCHC 6 cut(s) 34, 96, 121, 488, 630, 760
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 2 cut(s) 112, 475
SfoI GGCGCC 1 cut(s) 454
SinI GGWCC 2 cut(s) 105, 743
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
SseBI AGGCCT 1 cut(s) 416
SspDI GGCGCC 1 cut(s) 452
SspMI CTAG 1 cut(s) 468
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 1 cut(s) 233
StyI CCWWGG 2 cut(s) 6, 40
TaaI ACNGT 3 cut(s) 166, 175, 535
TaiI ACGT 1 cut(s) 582
TaqI TCGA 2 cut(s) 81, 87
TatI WGTACW 2 cut(s) 432, 783
TauI GCSGC 9 cut(s) 210, 213, 249, 450, 453, 770, 773, 800, 803
TfiI GAWTC 2 cut(s) 282, 660
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 4 cut(s) 383, 603, 731, 765
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 3 cut(s) 686, 764, 828
Tsp45I GTSAC 1 cut(s) 310
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 4 cut(s) 383, 603, 731, 765
Van91I CCANNNNNTGG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 105, 743
XagI CCTNNNNNAGG 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 267, 528
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.