MD10G1125200.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003977
Physical Location & Seq
Forward (+)
20494407 .. 20495879
1473 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1125200.v1.1.491

Sequence Viewer

Length: 987 bp
ATGTACCATTTTCCTCCCTTCTCCCTCTCTCTCTTTAAAACACGCACACCTAGAACCTCACTCTCCCAAAACCTCCAGAGCTTTCTTCCTTCCGCCGAAAAACCCAAGCCTTTTCTGAGAGTTCTCCACCTGTTTGTGAAAATGTCTAAGGAAGTAATCGAGGAGGGACAGGCCCGCCACCATGGGAAGGACTACGTCGACCCACCGCCAGCTCCTCTCTTTGACACGGACGAGCTCAAGCGCTGGTCCTTCTATAGGGCCCTCATCGCCGAGTTCATCGCCACTCTCCTCTTCCTCTACGTCACCGTAGCCACCGTCATCGGCCACAAGTCCTCACCCGGCGACTGCGGCGGCGTTGGCCTTCTGGGCATCGCCTGGGCCTTCGGCGGCATGATTTTCGTCCTCGTCTACTGCACCGCCGGAATCTCTGGTGGCCACATTAACCCAGCCGTGACTTTTGGTCTTTTCTTGGCCCGCAAGGTCTCCCTGATCAGAGCCGTGGCTTACATCGTTGCCCAGTGCTTGGGAGCCATCGCAGGCGTTGGGCTGGTCAAGGCCTTCCAGAAGCACAACTACAACTCCCTCGGCGGCGGCGCCAACTCCGTGGCTCCTGGCTACAACAAGGGCACTGCTCTGGGTGCTGAGATCATCGGCACCTTCGTGCTCGTTTACACCGTCTTCTCAGCCACCGACCCCAAGAGGAGCGCACGTGACTCCCACGTGCCTGTTTTGGCTCCACTGCCCATTGGGTTTGCTGTGTTTGTTGTGCACCTGGCAACAATCCCCATCACCGGAACTGGAATCAACCCAGCAAGGAGCTTCGGCGCTGCTGTGATCTACAACAACGAGAAAGTCTGGGATGACCACTGGATCTTCTGGGTCGGACCATTTGTGGGAGCACTGGCTGCGGCGGCGTACCACCAGTACATTCTGAGGGCGGCGGCGATCAAGGCGTTGGGATCATTCCGCAGCAACCCGACCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

329

Amino Acids

35.37

Weight (kDa)

9.59

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 78 - 307 1e-83 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 593, 653
AccB7I CCANNNNNTGG 1 cut(s) 523
AccI GTMKAC 2 cut(s) 198, 408
AclWI GGATC 2 cut(s) 878, 967
AcoI YGGCCR 2 cut(s) 322, 433
AcvI CACGTG 2 cut(s) 710, 721
AcyI GRCGYC 1 cut(s) 594
AfaI GTAC 3 cut(s) 5, 917, 926
AfeI AGCGCT 1 cut(s) 242
AfiI CCNNNNNNNGG 5 cut(s) 187, 255, 523, 791, 893
AhdI GACNNNNNGTC 1 cut(s) 459
AjnI CCWGG 3 cut(s) 374, 610, 771
AleI CACNNNNGTG 1 cut(s) 659
AluBI AGCT 4 cut(s) 81, 212, 235, 819
AluI AGCT 4 cut(s) 81, 212, 235, 819
Alw21I GWGCWC 4 cut(s) 237, 666, 771, 901
Alw26I GTCTC 1 cut(s) 487
Alw44I GTGCAC 1 cut(s) 767
AlwI GGATC 2 cut(s) 878, 967
Aor51HI AGCGCT 1 cut(s) 242
AoxI GGCC 8 cut(s) 171, 258, 322, 358, 378, 433, 471, 555
ApaI GGGCCC 1 cut(s) 262
ApaLI GTGCAC 1 cut(s) 767
ApeKI GCWGC 3 cut(s) 827, 905, 969
AspLEI GCGC 4 cut(s) 243, 596, 707, 827
AspS9I GGNCC 7 cut(s) 172, 246, 258, 259, 378, 472, 884
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 3 cut(s) 295, 327, 781
AvaII GGWCC 2 cut(s) 246, 884
BaeGI GKGCMC 3 cut(s) 262, 629, 771
BalI TGGCCA 1 cut(s) 435
BanI GGYRCC 2 cut(s) 593, 653
BanII GRGCYC 2 cut(s) 237, 262
BarI GAAGNNNNNNTAC 2 cut(s) 557, 589
BbrPI CACGTG 2 cut(s) 710, 721
BbsI GAAGAC 1 cut(s) 670
Bbv12I GWGCWC 4 cut(s) 237, 666, 771, 901
BbvI GCAGC 3 cut(s) 814, 892, 981
BccI CCATC 2 cut(s) 539, 794
BceAI ACGGC 2 cut(s) 434, 482
BcgI CGANNNNNNTGC 2 cut(s) 379, 413
BciT130I CCWGG 3 cut(s) 376, 612, 773
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 1 cut(s) 253
BfoI RGCGCY 3 cut(s) 244, 597, 828
BglI GCCNNNNNGGC 1 cut(s) 366
Bme1390I CCNGG 4 cut(s) 339, 376, 612, 773
Bme18I GGWCC 2 cut(s) 246, 884
BmeRI GACNNNNNGTC 1 cut(s) 459
BmgT120I GGNCC 7 cut(s) 172, 246, 258, 259, 378, 472, 884
BmiI GGNNCC 6 cut(s) 260, 529, 595, 609, 655, 735
BmrFI CCNGG 4 cut(s) 339, 376, 612, 773
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 1 cut(s) 378
BmuI ACTGGG 1 cut(s) 511
BpiI GAAGAC 1 cut(s) 670
BpmI CTGGAG 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 339
BsaAI YACGTR 2 cut(s) 710, 721
BsaHI GRCGYC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 5 cut(s) 181, 375, 498, 583, 603
BsaWI WCCGGW 1 cut(s) 791
BsaXI ACNNNNNCTCC 4 cut(s) 563, 593, 694, 724
Bsc4I CCNNNNNNNGG 5 cut(s) 187, 255, 523, 791, 893
Bse1I ACTGG 5 cut(s) 517, 802, 872, 906, 922
BseBI CCWGG 3 cut(s) 376, 612, 773
BseDI CCNNGG 5 cut(s) 181, 375, 498, 583, 603
BseGI GGATG 1 cut(s) 865
BseLI CCNNNNNNNGG 5 cut(s) 187, 255, 523, 791, 893
BseMII CTCAG 4 cut(s) 107, 633, 696, 923
BseNI ACTGG 5 cut(s) 517, 802, 872, 906, 922
BseRI GAGGAG 4 cut(s) 176, 204, 278, 715
BseSI GKGCMC 3 cut(s) 262, 629, 771
BseXI GCAGC 3 cut(s) 814, 892, 981
BseYI CCCAGC 2 cut(s) 445, 808
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 8 cut(s) 173, 260, 324, 360, 380, 435, 473, 557
BshNI GGYRCC 2 cut(s) 593, 653
BsiHKAI GWGCWC 4 cut(s) 237, 666, 771, 901
BsiSI CCGG 3 cut(s) 339, 420, 792
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 5 cut(s) 187, 255, 523, 791, 893
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 8 cut(s) 173, 260, 324, 360, 380, 435, 473, 557
Bso31I GGTCTC 1 cut(s) 487
Bsp120I GGGCCC 1 cut(s) 258
Bsp1286I GDGCHC 6 cut(s) 237, 262, 629, 666, 771, 901
Bsp143I GATC 6 cut(s) 489, 645, 834, 870, 945, 959
Bsp19I CCATGG 1 cut(s) 181
BspANI GGCC 8 cut(s) 173, 260, 324, 360, 380, 435, 473, 557
BspCNI CTCAG 4 cut(s) 108, 634, 695, 924
BspLI GGNNCC 6 cut(s) 260, 529, 595, 609, 655, 735
BspPI GGATC 2 cut(s) 878, 967
BspT107I GGYRCC 2 cut(s) 593, 653
BspTNI GGTCTC 1 cut(s) 487
BsrI ACTGG 5 cut(s) 517, 802, 872, 906, 922
BssECI CCNNGG 5 cut(s) 181, 375, 498, 583, 603
BssMI GATC 6 cut(s) 489, 645, 834, 870, 945, 959
BssNI GRCGYC 1 cut(s) 594
BssT1I CCWWGG 1 cut(s) 181
Bst2UI CCWGG 3 cut(s) 376, 612, 773
Bst4CI ACNGT 3 cut(s) 307, 316, 676
Bst6I CTCTTC 1 cut(s) 296
BstACI GRCGYC 1 cut(s) 594
BstAPI GCANNNNNTGC 1 cut(s) 905
BstBAI YACGTR 2 cut(s) 710, 721
BstC8I GCNNGC 4 cut(s) 175, 210, 475, 538
BstDEI CTNAG 5 cut(s) 116, 147, 642, 682, 932
BstDSI CCRYGG 3 cut(s) 181, 498, 603
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 865
BstH2I RGCGCY 3 cut(s) 244, 597, 828
BstHHI GCGC 4 cut(s) 243, 596, 707, 827
BstKTI GATC 6 cut(s) 492, 648, 837, 873, 948, 962
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 6 cut(s) 489, 645, 834, 870, 945, 959
BstMWI GCNNNNNNNGC 8 cut(s) 266, 348, 357, 366, 638, 905, 911, 950
BstNI CCWGG 3 cut(s) 376, 612, 773
BstSCI CCNGG 4 cut(s) 337, 374, 610, 771
BstSFI CTRYAG 1 cut(s) 253
BstSLI GKGCMC 3 cut(s) 262, 629, 771
BstV1I GCAGC 3 cut(s) 814, 892, 981
BstV2I GAAGAC 1 cut(s) 670
BstX2I RGATCY 1 cut(s) 870
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 1 cut(s) 870
BsuRI GGCC 8 cut(s) 173, 260, 324, 360, 380, 435, 473, 557
BtgI CCRYGG 3 cut(s) 181, 498, 603
BtgZI GCGATG 4 cut(s) 250, 262, 355, 517
BtsCI GGATG 1 cut(s) 865
BtsI GCAGTG 2 cut(s) 627, 737
BtsIMutI CAGTG 5 cut(s) 524, 627, 737, 865, 899
Cac8I GCNNGC 4 cut(s) 175, 210, 475, 538
CfoI GCGC 4 cut(s) 243, 596, 707, 827
Cfr13I GGNCC 7 cut(s) 172, 246, 258, 259, 378, 472, 884
Csp6I GTAC 3 cut(s) 4, 916, 925
CviAII CATG 2 cut(s) 182, 391
CviQI GTAC 3 cut(s) 4, 916, 925
DdeI CTNAG 5 cut(s) 116, 147, 642, 682, 932
DinI GGCGCC 1 cut(s) 595
DpnI GATC 6 cut(s) 491, 647, 836, 872, 947, 961
DpnII GATC 6 cut(s) 489, 645, 834, 870, 945, 959
DraI TTTAAA 1 cut(s) 37
DriI GACNNNNNGTC 1 cut(s) 459
EaeI YGGCCR 2 cut(s) 322, 433
Eam1104I CTCTTC 1 cut(s) 296
Eam1105I GACNNNNNGTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 296
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 181
Eco147I AGGCCT 1 cut(s) 557
Eco24I GRGCYC 2 cut(s) 237, 262
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 2 cut(s) 246, 884
Eco47III AGCGCT 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 235
Eco72I CACGTG 2 cut(s) 710, 721
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO109I RGGNCCY 2 cut(s) 258, 259
EcoRII CCWGG 3 cut(s) 374, 610, 771
EcoT14I CCWWGG 1 cut(s) 181
EcoT38I GRGCYC 2 cut(s) 237, 262
EgeI GGCGCC 1 cut(s) 595
EheI GGCGCC 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 2 cut(s) 185, 394
FaiI YATR 3 cut(s) 183, 255, 392
FaqI GGGAC 1 cut(s) 180
FatI CATG 2 cut(s) 181, 390
FauI CCCGC 2 cut(s) 182, 482
FbaI TGATCA 1 cut(s) 489
FblI GTMKAC 2 cut(s) 198, 408
FokI GGATG 1 cut(s) 872
FriOI GRGCYC 2 cut(s) 237, 262
FspBI CTAG 1 cut(s) 51
GlaI GCGC 4 cut(s) 242, 595, 706, 826
GsaI CCCAGC 2 cut(s) 449, 812
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 3 cut(s) 244, 597, 828
HaeIII GGCC 8 cut(s) 173, 260, 324, 360, 380, 435, 473, 557
HapII CCGG 3 cut(s) 339, 420, 792
HhaI GCGC 4 cut(s) 243, 596, 707, 827
Hin1I GRCGYC 1 cut(s) 594
Hin1II CATG 2 cut(s) 185, 394
Hin6I GCGC 4 cut(s) 241, 594, 705, 825
HinP1I GCGC 4 cut(s) 241, 594, 705, 825
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 3 cut(s) 423, 713, 801
HpaII CCGG 3 cut(s) 339, 420, 792
HphI GGTGA 3 cut(s) 295, 327, 781
Hpy166II GTNNAC 4 cut(s) 199, 409, 670, 769
Hpy188I TCNGA 4 cut(s) 117, 494, 884, 933
Hpy188III TCNNGA 2 cut(s) 76, 562
Hpy8I GTNNAC 4 cut(s) 199, 409, 670, 769
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 8 cut(s) 28, 99, 181, 259, 371, 391, 568, 667
HpyCH4III ACNGT 3 cut(s) 307, 316, 676
HpyCH4IV ACGT 4 cut(s) 195, 300, 709, 720
HpyCH4V TGCA 2 cut(s) 414, 769
HpyF10VI GCNNNNNNNGC 8 cut(s) 266, 348, 357, 366, 638, 905, 911, 950
HpyF3I CTNAG 5 cut(s) 116, 147, 642, 682, 932
HpySE526I ACGT 4 cut(s) 195, 300, 709, 720
Hsp92I GRCGYC 1 cut(s) 594
Hsp92II CATG 2 cut(s) 185, 394
HspAI GCGC 4 cut(s) 241, 594, 705, 825
KasI GGCGCC 1 cut(s) 593
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 6 cut(s) 489, 645, 834, 870, 945, 959
LmnI GCTCC 7 cut(s) 217, 527, 613, 702, 739, 816, 896
Lsp1109I GCAGC 3 cut(s) 814, 892, 981
LweI GCATC 1 cut(s) 378
MaeI CTAG 1 cut(s) 51
MaeII ACGT 4 cut(s) 195, 300, 709, 720
MaeIII GTNAC 3 cut(s) 301, 451, 710
MalI GATC 6 cut(s) 491, 647, 836, 872, 947, 961
MboI GATC 6 cut(s) 489, 645, 834, 870, 945, 959
MboII GAAGA 4 cut(s) 77, 283, 670, 865
MflI RGATCY 1 cut(s) 870
MhlI GDGCHC 6 cut(s) 237, 262, 629, 666, 771, 901
MlsI TGGCCA 1 cut(s) 435
MluNI TGGCCA 1 cut(s) 435
Mly113I GGCGCC 1 cut(s) 594
MlyI GAGTC 1 cut(s) 707
MmeI TCCRAC 1 cut(s) 862
Mox20I TGGCCA 1 cut(s) 435
MscI TGGCCA 1 cut(s) 435
MseI TTAA 2 cut(s) 36, 441
MslI CAYNNNNRTG 1 cut(s) 659
Msp20I TGGCCA 1 cut(s) 435
MspI CCGG 3 cut(s) 339, 420, 792
MspR9I CCNGG 4 cut(s) 339, 376, 612, 773
MvaI CCWGG 3 cut(s) 376, 612, 773
MwoI GCNNNNNNNGC 8 cut(s) 266, 348, 357, 366, 638, 905, 911, 950
NarI GGCGCC 1 cut(s) 594
NciI CCSGG 1 cut(s) 339
NcoI CCATGG 1 cut(s) 181
NdeII GATC 6 cut(s) 489, 645, 834, 870, 945, 959
NlaIII CATG 2 cut(s) 185, 394
NlaIV GGNNCC 6 cut(s) 260, 529, 595, 609, 655, 735
NmeAIII GCCGAG 2 cut(s) 295, 564
NmuCI GTSAC 3 cut(s) 301, 451, 710
OliI CACNNNNGTG 1 cut(s) 659
PceI AGGCCT 1 cut(s) 557
PcsI WCGNNNNNNNCGW 2 cut(s) 657, 672
PfeI GAWTC 2 cut(s) 423, 801
PflFI GACNNNGTC 1 cut(s) 194
PflMI CCANNNNNTGG 1 cut(s) 523
PleI GAGTC 1 cut(s) 707
PluTI GGCGCC 1 cut(s) 597
PmaCI CACGTG 2 cut(s) 710, 721
PmlI CACGTG 2 cut(s) 710, 721
PpsI GAGTC 1 cut(s) 707
Ppu21I YACGTR 2 cut(s) 710, 721
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 3 cut(s) 374, 610, 771
PspCI CACGTG 2 cut(s) 710, 721
PspFI CCCAGC 2 cut(s) 445, 808
PspGI CCWGG 3 cut(s) 374, 610, 771
PspN4I GGNNCC 6 cut(s) 260, 529, 595, 609, 655, 735
PspOMI GGGCCC 1 cut(s) 258
PspPI GGNCC 7 cut(s) 172, 246, 258, 259, 378, 472, 884
PsuI RGATCY 1 cut(s) 870
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 3 cut(s) 5, 917, 926
RsaNI GTAC 3 cut(s) 4, 916, 925
RseI CAYNNNNRTG 1 cut(s) 659
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 36, 441
Sau3AI GATC 6 cut(s) 489, 645, 834, 870, 945, 959
Sau96I GGNCC 7 cut(s) 172, 246, 258, 259, 378, 472, 884
SchI GAGTC 1 cut(s) 707
ScrFI CCNGG 4 cut(s) 339, 376, 612, 773
SduI GDGCHC 6 cut(s) 237, 262, 629, 666, 771, 901
SfaNI GCATC 1 cut(s) 378
SfcI CTRYAG 1 cut(s) 253
SfoI GGCGCC 1 cut(s) 595
SinI GGWCC 2 cut(s) 246, 884
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SseBI AGGCCT 1 cut(s) 557
SspDI GGCGCC 1 cut(s) 593
SspMI CTAG 1 cut(s) 51
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 557
StyD4I CCNGG 4 cut(s) 337, 374, 610, 771
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 3 cut(s) 307, 316, 676
TaiI ACGT 4 cut(s) 198, 303, 712, 723
TaqI TCGA 2 cut(s) 159, 198
TatI WGTACW 1 cut(s) 924
TauI GCSGC 9 cut(s) 351, 354, 390, 591, 594, 911, 914, 941, 944
TfiI GAWTC 2 cut(s) 423, 801
Tru1I TTAA 2 cut(s) 36, 441
Tru9I TTAA 2 cut(s) 36, 441
TscAI CASTG 5 cut(s) 524, 634, 744, 872, 906
TseFI GTSAC 3 cut(s) 301, 451, 710
TseI GCWGC 3 cut(s) 827, 905, 969
Tsp45I GTSAC 3 cut(s) 301, 451, 710
TspDTI ATGAA 1 cut(s) 265
TspGWI ACGGA 2 cut(s) 242, 592
TspRI CASTG 5 cut(s) 524, 634, 744, 872, 906
Tth111I GACNNNGTC 1 cut(s) 194
Van91I CCANNNNNTGG 1 cut(s) 523
VneI GTGCAC 1 cut(s) 767
VpaK11BI GGWCC 2 cut(s) 246, 884
XagI CCTNNNNNAGG 1 cut(s) 253
XmiI GTMKAC 2 cut(s) 198, 408
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.