FvH4_2g15440

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003977
Physical Location & Seq
Forward (+)
13587024 .. 13589165
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15440.t3

Sequence Viewer

Length: 846 bp
ATGTCGAAGGAAGTCAGCGAGGAGGCGCAGGCCCACCACCACGGCAAGGACTACGTCGACCCACCGCCGGCGCCGCTCTTCGACACCGACGAGCTCAAGCGCTGGTCCTTCTACAGGGCCCTCATCGCCGAGTTCGTCGCCACTCTCCTCTTCCTCTACATCACTGTCGCCACCGTCATCGGCCACAAGCACCAGACCGGCCCTTGCGACGGCGTCGGCCTTCTCGGCATCGCCTGGGCCTTCGGTGGCATGATCTTTGTCCTGGTTTACTGCACCGCCGGTATTTCCGGTGGTCACATTAACCCGGCGGTGACTTTCGGGCTGTTCTTGGCTCGTAAGGTTTCTCTCATCCGGGCCGTGTCTTACATGATCGCCCAGTGCTTGGGAGCCATCGCTGGAGTTGCGCTGGTCAAGGCCTTCCAGAGTCACTACTTCACCACCCTCGGCGGCGGCACCAACTCTGTCGCTGACGGTTACAGCAAGGGCACTGCTCTCGGCGCTGAGATTATCGGCACCTTTGTGCTTGTCTACACCGTCTTCTCTGCCACCGACCCCAAGAGGAGCGCGCGTGACTCTCACGTGCCTGTGTTGGCTCCATTGCCTATTGGGTTTGCTGTGTTCATTGTGCACTTGGCTACCATCCCTATCACCGGAACTGGAATCAACCCTGCTCGGAGCTTCGGCGCTGCCGTCATCTACAACAACGAGAAAGTCTGGGATGAACATTGGATCTTCTGGGTCGGACCCTTTATCGGAGCTCTTGCCGCCGCAGCATACCACCAGTACATTCTGAGAGCCGCCGCCATCAAAGCTTTGGGATCATTCCGGAGCAACCCCTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

282

Amino Acids

30.04

Weight (kDa)

8.38

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 243 5.7e-74 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 70, 452, 512
AccB7I CCANNNNNTGG 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 2 cut(s) 57, 528
AccII CGCG 2 cut(s) 566, 568
AccIII TCCGGA 1 cut(s) 825
AclWI GGATC 2 cut(s) 737, 826
AcoI YGGCCR 1 cut(s) 181
AcvI CACGTG 1 cut(s) 580
AcyI GRCGYC 2 cut(s) 71, 213
AfaI GTAC 1 cut(s) 785
AfeI AGCGCT 1 cut(s) 101
AfiI CCNNNNNNNGG 7 cut(s) 46, 67, 114, 209, 382, 650, 752
AjnI CCWGG 2 cut(s) 233, 261
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 4 cut(s) 94, 678, 758, 812
AluI AGCT 4 cut(s) 94, 678, 758, 812
Alw21I GWGCWC 3 cut(s) 96, 630, 760
Alw44I GTGCAC 1 cut(s) 626
AlwI GGATC 2 cut(s) 737, 826
Aor13HI TCCGGA 1 cut(s) 825
Aor51HI AGCGCT 1 cut(s) 101
AoxI GGCC 8 cut(s) 30, 117, 181, 199, 217, 237, 354, 414
ApaI GGGCCC 1 cut(s) 121
ApaLI GTGCAC 1 cut(s) 626
ApeKI GCWGC 2 cut(s) 686, 770
AspLEI GCGC 8 cut(s) 28, 73, 102, 406, 500, 566, 568, 686
AspS9I GGNCC 8 cut(s) 31, 105, 117, 118, 200, 237, 354, 743
AsuC2I CCSGG 2 cut(s) 305, 353
AsuHPI GGTGA 3 cut(s) 322, 427, 640
AvaII GGWCC 2 cut(s) 105, 743
BaeGI GKGCMC 3 cut(s) 121, 488, 630
BanI GGYRCC 3 cut(s) 70, 452, 512
BanII GRGCYC 3 cut(s) 96, 121, 760
BbrPI CACGTG 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 3 cut(s) 96, 630, 760
BbvI GCAGC 2 cut(s) 673, 782
BccI CCATC 3 cut(s) 398, 647, 812
BceAI ACGGC 4 cut(s) 58, 226, 341, 674
BcgI CGANNNNNNTGC 2 cut(s) 475, 509
BciT130I CCWGG 2 cut(s) 235, 263
BcnI CCSGG 2 cut(s) 305, 353
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 4 cut(s) 74, 103, 501, 687
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 9 cut(s) 74, 448, 451, 687, 765, 768, 771, 798, 801
BlsI GCNGC 9 cut(s) 75, 449, 452, 688, 766, 769, 772, 799, 802
Bme1390I CCNGG 4 cut(s) 235, 263, 305, 353
Bme18I GGWCC 2 cut(s) 105, 743
BmgT120I GGNCC 8 cut(s) 31, 105, 117, 118, 200, 237, 354, 743
BmiI GGNNCC 7 cut(s) 72, 119, 388, 454, 514, 594, 745
BmrFI CCNGG 4 cut(s) 235, 263, 305, 353
BmrI ACTGGG 1 cut(s) 370
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 529
BpmI CTGGAG 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 305, 353
BsaAI YACGTR 1 cut(s) 580
BsaHI GRCGYC 2 cut(s) 71, 213
BsaJI CCNNGG 3 cut(s) 40, 234, 442
BsaWI WCCGGW 3 cut(s) 287, 650, 825
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 67, 114, 209, 382, 650, 752
Bse118I RCCGGY 3 cut(s) 67, 197, 278
Bse1I ACTGG 3 cut(s) 376, 661, 781
Bse3DI GCAATG 1 cut(s) 596
BseAI TCCGGA 1 cut(s) 825
BseBI CCWGG 2 cut(s) 235, 263
BseDI CCNNGG 3 cut(s) 40, 234, 442
BseGI GGATG 3 cut(s) 348, 639, 724
BseLI CCNNNNNNNGG 7 cut(s) 46, 67, 114, 209, 382, 650, 752
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 2 cut(s) 492, 782
BseNI ACTGG 3 cut(s) 376, 661, 781
BsePI GCGCGC 1 cut(s) 564
BseRI GAGGAG 3 cut(s) 35, 137, 574
BseSI GKGCMC 3 cut(s) 121, 488, 630
BseXI GCAGC 2 cut(s) 673, 782
BsgI GTGCAG 1 cut(s) 256
Bsh1236I CGCG 2 cut(s) 566, 568
BshFI GGCC 8 cut(s) 32, 119, 183, 201, 219, 239, 356, 416
BshNI GGYRCC 3 cut(s) 70, 452, 512
BsiHKAI GWGCWC 3 cut(s) 96, 630, 760
BsiSI CCGG 8 cut(s) 68, 198, 279, 288, 305, 352, 651, 826
BslI CCNNNNNNNGG 7 cut(s) 46, 67, 114, 209, 382, 650, 752
BsnI GGCC 8 cut(s) 32, 119, 183, 201, 219, 239, 356, 416
Bsp120I GGGCCC 1 cut(s) 117
Bsp1286I GDGCHC 5 cut(s) 96, 121, 488, 630, 760
Bsp13I TCCGGA 1 cut(s) 825
Bsp143I GATC 4 cut(s) 252, 369, 729, 818
BspANI GGCC 8 cut(s) 32, 119, 183, 201, 219, 239, 356, 416
BspCNI CTCAG 2 cut(s) 493, 783
BspEI TCCGGA 1 cut(s) 825
BspFNI CGCG 2 cut(s) 566, 568
BspLI GGNNCC 7 cut(s) 72, 119, 388, 454, 514, 594, 745
BspPI GGATC 2 cut(s) 737, 826
BspQI GCTCTTC 1 cut(s) 83
BspT107I GGYRCC 3 cut(s) 70, 452, 512
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 596
BsrFI RCCGGY 3 cut(s) 67, 197, 278
BsrI ACTGG 3 cut(s) 376, 661, 781
BssAI RCCGGY 3 cut(s) 67, 197, 278
BssECI CCNNGG 3 cut(s) 40, 234, 442
BssHII GCGCGC 1 cut(s) 564
BssMI GATC 4 cut(s) 252, 369, 729, 818
BssNI GRCGYC 2 cut(s) 71, 213
Bst2UI CCWGG 2 cut(s) 235, 263
Bst4CI ACNGT 4 cut(s) 166, 175, 473, 535
Bst6I CTCTTC 2 cut(s) 83, 155
BstACI GRCGYC 2 cut(s) 71, 213
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 30, 69, 566
BstDEI CTNAG 2 cut(s) 501, 791
BstDSI CCRYGG 1 cut(s) 40
BstENI CCTNNNNNAGG 1 cut(s) 112
BstF5I GGATG 3 cut(s) 348, 639, 724
BstFNI CGCG 2 cut(s) 566, 568
BstH2I RGCGCY 4 cut(s) 74, 103, 501, 687
BstHHI GCGC 8 cut(s) 28, 73, 102, 406, 500, 566, 568, 686
BstKTI GATC 4 cut(s) 255, 372, 732, 821
BstMBI GATC 4 cut(s) 252, 369, 729, 818
BstMWI GCNNNNNNNGC 8 cut(s) 73, 125, 225, 401, 497, 764, 770, 809
BstNI CCWGG 2 cut(s) 235, 263
BstSCI CCNGG 4 cut(s) 233, 261, 303, 351
BstSFI CTRYAG 1 cut(s) 112
BstSLI GKGCMC 3 cut(s) 121, 488, 630
BstUI CGCG 2 cut(s) 566, 568
BstV1I GCAGC 2 cut(s) 673, 782
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 729
BstYI RGATCY 1 cut(s) 729
BsuRI GGCC 8 cut(s) 32, 119, 183, 201, 219, 239, 356, 416
BtgI CCRYGG 1 cut(s) 40
BtgZI GCGATG 3 cut(s) 109, 214, 376
BtsCI GGATG 3 cut(s) 348, 639, 724
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 3 cut(s) 162, 383, 486
Cac8I GCNNGC 3 cut(s) 30, 69, 566
CfoI GCGC 8 cut(s) 28, 73, 102, 406, 500, 566, 568, 686
Cfr10I RCCGGY 3 cut(s) 67, 197, 278
Cfr13I GGNCC 8 cut(s) 31, 105, 117, 118, 200, 237, 354, 743
CseI GACGC 1 cut(s) 202
Csp6I GTAC 1 cut(s) 784
CviAII CATG 2 cut(s) 250, 367
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 2 cut(s) 501, 791
DinI GGCGCC 1 cut(s) 72
DpnI GATC 4 cut(s) 254, 371, 731, 820
DpnII GATC 4 cut(s) 252, 369, 729, 818
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 2 cut(s) 83, 155
EarI CTCTTC 2 cut(s) 83, 155
Ecl136II GAGCTC 2 cut(s) 94, 758
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 3 cut(s) 96, 121, 760
Eco47I GGWCC 2 cut(s) 105, 743
Eco47III AGCGCT 1 cut(s) 101
Eco53kI GAGCTC 2 cut(s) 94, 758
Eco72I CACGTG 1 cut(s) 580
EcoICRI GAGCTC 2 cut(s) 94, 758
EcoNI CCTNNNNNAGG 1 cut(s) 112
EcoO109I RGGNCCY 2 cut(s) 117, 118
EcoRII CCWGG 2 cut(s) 233, 261
EcoT38I GRGCYC 3 cut(s) 96, 121, 760
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
FaeI CATG 2 cut(s) 253, 370
FaiI YATR 3 cut(s) 251, 368, 775
FatI CATG 2 cut(s) 249, 366
FblI GTMKAC 2 cut(s) 57, 528
Fnu4HI GCNGC 9 cut(s) 74, 448, 451, 687, 765, 768, 771, 798, 801
FokI GGATG 3 cut(s) 335, 626, 731
FriOI GRGCYC 3 cut(s) 96, 121, 760
Fsp4HI GCNGC 9 cut(s) 74, 448, 451, 687, 765, 768, 771, 798, 801
GlaI GCGC 8 cut(s) 27, 72, 101, 405, 499, 565, 567, 685
GluI GCNGC 9 cut(s) 74, 448, 451, 687, 765, 768, 771, 798, 801
GsuI CTGGAG 1 cut(s) 417
HaeII RGCGCY 4 cut(s) 74, 103, 501, 687
HaeIII GGCC 8 cut(s) 32, 119, 183, 201, 219, 239, 356, 416
HapII CCGG 8 cut(s) 68, 198, 279, 288, 305, 352, 651, 826
HgaI GACGC 1 cut(s) 202
HhaI GCGC 8 cut(s) 28, 73, 102, 406, 500, 566, 568, 686
Hin1I GRCGYC 2 cut(s) 71, 213
Hin1II CATG 2 cut(s) 253, 370
Hin6I GCGC 8 cut(s) 26, 71, 100, 404, 498, 564, 566, 684
HinP1I GCGC 8 cut(s) 26, 71, 100, 404, 498, 564, 566, 684
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 3 cut(s) 424, 572, 660
HpaII CCGG 8 cut(s) 68, 198, 279, 288, 305, 352, 651, 826
HphI GGTGA 3 cut(s) 322, 427, 640
Hpy166II GTNNAC 4 cut(s) 58, 268, 529, 628
Hpy188I TCNGA 4 cut(s) 675, 743, 755, 792
Hpy188III TCNNGA 2 cut(s) 421, 826
Hpy8I GTNNAC 4 cut(s) 58, 268, 529, 628
Hpy99I CGWCG 5 cut(s) 59, 92, 140, 212, 218
HpyAV CCTTC 4 cut(s) 118, 230, 250, 427
HpyCH4III ACNGT 4 cut(s) 166, 175, 473, 535
HpyCH4IV ACGT 2 cut(s) 54, 579
HpyCH4V TGCA 2 cut(s) 273, 628
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 125, 225, 401, 497, 764, 770, 809
HpyF3I CTNAG 2 cut(s) 501, 791
HpySE526I ACGT 2 cut(s) 54, 579
Hsp92I GRCGYC 2 cut(s) 71, 213
Hsp92II CATG 2 cut(s) 253, 370
HspAI GCGC 8 cut(s) 26, 71, 100, 404, 498, 564, 566, 684
KasI GGCGCC 1 cut(s) 70
Kpn2I TCCGGA 1 cut(s) 825
KroI GCCGGC 1 cut(s) 67
KroNI GCCGGC 1 cut(s) 69
Kzo9I GATC 4 cut(s) 252, 369, 729, 818
LguI GCTCTTC 1 cut(s) 83
LmnI GCTCC 6 cut(s) 386, 561, 598, 675, 755, 828
Lsp1109I GCAGC 2 cut(s) 673, 782
LweI GCATC 1 cut(s) 237
MaeII ACGT 2 cut(s) 54, 579
MaeIII GTNAC 5 cut(s) 293, 310, 425, 473, 569
MalI GATC 4 cut(s) 254, 371, 731, 820
MbiI CCGCTC 1 cut(s) 76
MboI GATC 4 cut(s) 252, 369, 729, 818
MboII GAAGA 4 cut(s) 70, 142, 529, 724
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 5 cut(s) 96, 121, 488, 630, 760
Mly113I GGCGCC 1 cut(s) 71
MlyI GAGTC 2 cut(s) 433, 566
MmeI TCCRAC 1 cut(s) 721
MnlI CCTC 7 cut(s) 13, 16, 131, 158, 164, 452, 552
MreI CGCCGGCG 1 cut(s) 67
MroI TCCGGA 1 cut(s) 825
MroNI GCCGGC 1 cut(s) 67
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 518
MspI CCGG 8 cut(s) 68, 198, 279, 288, 305, 352, 651, 826
MspR9I CCNGG 4 cut(s) 235, 263, 305, 353
MvaI CCWGG 2 cut(s) 235, 263
MvnI CGCG 2 cut(s) 566, 568
MwoI GCNNNNNNNGC 8 cut(s) 73, 125, 225, 401, 497, 764, 770, 809
NaeI GCCGGC 1 cut(s) 69
NarI GGCGCC 1 cut(s) 71
NciI CCSGG 2 cut(s) 305, 353
NdeII GATC 4 cut(s) 252, 369, 729, 818
NgoMIV GCCGGC 1 cut(s) 67
NlaIII CATG 2 cut(s) 253, 370
NlaIV GGNNCC 7 cut(s) 72, 119, 388, 454, 514, 594, 745
NmeAIII GCCGAG 4 cut(s) 154, 204, 423, 474
NmuCI GTSAC 4 cut(s) 293, 310, 425, 569
OliI CACNNNNGTG 1 cut(s) 518
PauI GCGCGC 1 cut(s) 564
PceI AGGCCT 1 cut(s) 416
PciSI GCTCTTC 1 cut(s) 83
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 132, 687
PdiI GCCGGC 1 cut(s) 69
PfeI GAWTC 1 cut(s) 660
PflFI GACNNNGTC 2 cut(s) 53, 212
PflMI CCANNNNNTGG 1 cut(s) 382
PkrI GCNGC 9 cut(s) 75, 449, 452, 688, 766, 769, 772, 799, 802
PleI GAGTC 2 cut(s) 432, 566
PluTI GGCGCC 1 cut(s) 74
PmaCI CACGTG 1 cut(s) 580
PmlI CACGTG 1 cut(s) 580
PpsI GAGTC 2 cut(s) 432, 566
Ppu21I YACGTR 1 cut(s) 580
Psp124BI GAGCTC 2 cut(s) 96, 760
Psp6I CCWGG 2 cut(s) 233, 261
PspCI CACGTG 1 cut(s) 580
PspGI CCWGG 2 cut(s) 233, 261
PspN4I GGNNCC 7 cut(s) 72, 119, 388, 454, 514, 594, 745
PspOMI GGGCCC 1 cut(s) 117
PspPI GGNCC 8 cut(s) 31, 105, 117, 118, 200, 237, 354, 743
PsuI RGATCY 1 cut(s) 729
PsyI GACNNNGTC 2 cut(s) 53, 212
PteI GCGCGC 1 cut(s) 564
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 2 cut(s) 96, 760
SalI GTCGAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 83
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 9 cut(s) 74, 448, 451, 687, 765, 768, 771, 798, 801
Sau3AI GATC 4 cut(s) 252, 369, 729, 818
Sau96I GGNCC 8 cut(s) 31, 105, 117, 118, 200, 237, 354, 743
SchI GAGTC 2 cut(s) 433, 566
ScrFI CCNGG 4 cut(s) 235, 263, 305, 353
SduI GDGCHC 5 cut(s) 96, 121, 488, 630, 760
SetI ASST 8 cut(s) 57, 96, 342, 518, 582, 680, 760, 814
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 112
SfoI GGCGCC 1 cut(s) 72
SgrAI CRCCGGYG 1 cut(s) 67
SinI GGWCC 2 cut(s) 105, 743
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 416
SspDI GGCGCC 1 cut(s) 70
SstI GAGCTC 2 cut(s) 96, 760
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 4 cut(s) 233, 261, 303, 351
TaaI ACNGT 4 cut(s) 166, 175, 473, 535
TaiI ACGT 2 cut(s) 57, 582
TaqI TCGA 3 cut(s) 5, 57, 81
TatI WGTACW 1 cut(s) 783
TauI GCSGC 7 cut(s) 76, 450, 453, 767, 770, 800, 803
TfiI GAWTC 1 cut(s) 660
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 3 cut(s) 169, 383, 493
TseFI GTSAC 4 cut(s) 293, 310, 425, 569
TseI GCWGC 2 cut(s) 686, 770
Tsp45I GTSAC 4 cut(s) 293, 310, 425, 569
TspDTI ATGAA 2 cut(s) 610, 735
TspRI CASTG 3 cut(s) 169, 383, 493
Tth111I GACNNNGTC 2 cut(s) 53, 212
Van91I CCANNNNNTGG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 105, 743
XagI CCTNNNNNAGG 1 cut(s) 112
XcmI CCANNNNNNNNNTGG 1 cut(s) 811
XmiI GTMKAC 2 cut(s) 57, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.