Rroxscaffold_2G00143820

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007936
Physical Location & Seq
Forward (+)
81773375 .. 81774775
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143820.1

Sequence Viewer

Length: 669 bp
ATGGAAGGCGGTGAATCGGAGATGACGACAGTATCACGTCATCATTTCGGAGATGACTCGGACCGACCGGACTTGTCTATACCCATAATCGAGAATATGAAAGAAGACTATGGGCTGTTCGTGTGGCCTTGCAGTATAGTCCTGGCGGAGTATGTTTGGCAGCAAAGACTGCGTTTTGCCGGCGCTAGTGTGGTTGAGCTTGGTGCTGGAACTTCTTTGCCTGGTTTGGTTGCCGCCAAAGTGGGCGCTGACGTCACTCTTACTGATGAATCCAATAGGTTGGAGGTGCTGAGCAACATGAAAAGTGTTATTGAACTTAACAAACTTCAATGCAATGTAGTAGGATTGACATGGGGAGTTTGGGATGCATCTATATTCAGTTTACACCCGAAAATTATTCTTGGGGCTGATGTATTGTATGATGCTAAAGCCTTTGATGACCTCTTTGCCACTGTGGCATATCTGCTCCAGAATTCTCCTGGTTCGGTCTTCATAACAACATACCATAATCGAAGTGGGCATCATCTTATCGAATTCTTGATGGTCAAATGGGGATTGAAGTGTTTGAAACTTCTAGATGCGTTTTCATTTATGCCATCCAGCAAGGCATCTGGGCTAAGTGGAAACCTTCAATTGGCAGAGATTGTTCTGGACAGTGAACATCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

222

Amino Acids

24.45

Weight (kDa)

5.07

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 25 - 172 2.1e-22 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
AciI CCGC 3 cut(s) 9, 146, 234
AcsI RAATTY 2 cut(s) 472, 533
AcyI GRCGYC 1 cut(s) 252
AfiI CCNNNNNNNGG 1 cut(s) 634
AgsI TTSAA 5 cut(s) 314, 329, 559, 568, 632
AjiI CACGTC 1 cut(s) 38
AjnI CCWGG 3 cut(s) 141, 220, 478
AluBI AGCT 1 cut(s) 199
AluI AGCT 1 cut(s) 199
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 2 cut(s) 472, 533
AspLEI GCGC 2 cut(s) 185, 248
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 23
AvaII GGWCC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 15, 48
BbsI GAAGAC 2 cut(s) 111, 481
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 535, 604
BciT130I CCWGG 3 cut(s) 143, 222, 480
BfaI CTAG 2 cut(s) 186, 575
BfoI RGCGCY 2 cut(s) 186, 249
BglI GCCNNNNNGGC 1 cut(s) 455
BisI GCNGC 2 cut(s) 161, 234
BlpI GCTNAGC 1 cut(s) 290
BlsI GCNGC 2 cut(s) 162, 235
Bme1390I CCNGG 3 cut(s) 143, 222, 480
Bme18I GGWCC 1 cut(s) 61
BmgBI CACGTC 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 61
BmrFI CCNGG 3 cut(s) 143, 222, 480
BmsI GCATC 6 cut(s) 355, 377, 412, 529, 568, 617
BpiI GAAGAC 2 cut(s) 111, 481
BpmI CTGGAG 1 cut(s) 452
Bpu1102I GCTNAGC 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse118I RCCGGY 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 340
BseBI CCWGG 3 cut(s) 143, 222, 480
BseGI GGATG 2 cut(s) 370, 596
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 1 cut(s) 281
BseXI GCAGC 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 68
BsiSI CCGG 2 cut(s) 68, 180
BslI CCNNNNNNNGG 1 cut(s) 634
BsnI GGCC 1 cut(s) 127
Bsp1720I GCTNAGC 1 cut(s) 290
BspACI CCGC 3 cut(s) 9, 146, 234
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 179
BssAI RCCGGY 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 252
Bst2UI CCWGG 3 cut(s) 143, 222, 480
Bst4CI ACNGT 3 cut(s) 31, 454, 656
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 2 cut(s) 290, 617
BstF5I GGATG 2 cut(s) 370, 596
BstH2I RGCGCY 2 cut(s) 186, 249
BstHHI GCGC 2 cut(s) 185, 248
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 2 cut(s) 169, 455
BstNI CCWGG 3 cut(s) 143, 222, 480
BstSCI CCNGG 3 cut(s) 141, 220, 478
BstV1I GCAGC 1 cut(s) 172
BstV2I GAAGAC 2 cut(s) 111, 481
BstXI CCANNNNNNTGG 1 cut(s) 280
BsuRI GGCC 1 cut(s) 127
BtgZI GCGATG 1 cut(s) 647
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 2 cut(s) 370, 596
BtsIMutI CAGTG 2 cut(s) 450, 661
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 2 cut(s) 185, 248
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 61
CpoI CGGWCCG 1 cut(s) 61
CspI CGGWCCG 1 cut(s) 61
CviAII CATG 2 cut(s) 298, 351
CviJI RGCY 6 cut(s) 115, 127, 199, 407, 431, 616
CviKI_1 RGCY 6 cut(s) 115, 127, 199, 407, 431, 616
DdeI CTNAG 2 cut(s) 290, 617
EciI GGCGGA 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 61
EcoRI GAATTC 2 cut(s) 472, 533
EcoRII CCWGG 3 cut(s) 141, 220, 478
EcoT22I ATGCAT 1 cut(s) 370
FaeI CATG 2 cut(s) 301, 354
FatI CATG 2 cut(s) 297, 350
Fnu4HI GCNGC 2 cut(s) 161, 234
FokI GGATG 2 cut(s) 377, 583
Fsp4HI GCNGC 2 cut(s) 161, 234
FspBI CTAG 2 cut(s) 186, 575
GlaI GCGC 2 cut(s) 184, 247
GluI GCNGC 2 cut(s) 161, 234
GsuI CTGGAG 1 cut(s) 452
HaeII RGCGCY 2 cut(s) 186, 249
HaeIII GGCC 1 cut(s) 127
HapII CCGG 2 cut(s) 68, 180
HhaI GCGC 2 cut(s) 185, 248
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 2 cut(s) 301, 354
Hin6I GCGC 2 cut(s) 183, 246
HinP1I GCGC 2 cut(s) 183, 246
HinfI GANTC 3 cut(s) 14, 56, 269
HpaII CCGG 2 cut(s) 68, 180
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 2 cut(s) 383, 659
Hpy188I TCNGA 3 cut(s) 19, 50, 61
Hpy188III TCNNGA 5 cut(s) 91, 469, 538, 575, 650
Hpy8I GTNNAC 2 cut(s) 383, 659
HpyAV CCTTC 1 cut(s) 638
HpyCH4III ACNGT 3 cut(s) 31, 454, 656
HpyCH4IV ACGT 2 cut(s) 37, 252
HpyCH4V TGCA 3 cut(s) 132, 333, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 169, 455
HpyF3I CTNAG 2 cut(s) 290, 617
HpySE526I ACGT 2 cut(s) 37, 252
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 2 cut(s) 301, 354
HspAI GCGC 2 cut(s) 183, 246
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 6 cut(s) 355, 377, 412, 529, 568, 617
MaeI CTAG 2 cut(s) 186, 575
MaeII ACGT 2 cut(s) 37, 252
MaeIII GTNAC 1 cut(s) 253
MboII GAAGA 2 cut(s) 116, 481
MfeI CAATTG 1 cut(s) 632
MluCI AATT 4 cut(s) 393, 472, 533, 632
MlyI GAGTC 1 cut(s) 50
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 2 cut(s) 277, 452
Mph1103I ATGCAT 1 cut(s) 370
MroNI GCCGGC 1 cut(s) 179
MseI TTAA 1 cut(s) 318
MspI CCGG 2 cut(s) 68, 180
MspR9I CCNGG 3 cut(s) 143, 222, 480
MunI CAATTG 1 cut(s) 632
MvaI CCWGG 3 cut(s) 143, 222, 480
MwoI GCNNNNNNNGC 2 cut(s) 169, 455
NaeI GCCGGC 1 cut(s) 181
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 2 cut(s) 301, 354
NmuCI GTSAC 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PdiI GCCGGC 1 cut(s) 181
PfeI GAWTC 2 cut(s) 14, 269
PkrI GCNGC 2 cut(s) 162, 235
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Psp6I CCWGG 3 cut(s) 141, 220, 478
PspGI CCWGG 3 cut(s) 141, 220, 478
PspPI GGNCC 1 cut(s) 61
Rsr2I CGGWCCG 1 cut(s) 61
RsrII CGGWCCG 1 cut(s) 61
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 2 cut(s) 161, 234
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 3 cut(s) 143, 222, 480
SetI ASST 7 cut(s) 40, 201, 255, 281, 288, 444, 630
SfaNI GCATC 6 cut(s) 355, 377, 412, 529, 568, 617
SinI GGWCC 1 cut(s) 61
Sse9I AATT 4 cut(s) 393, 472, 533, 632
SsiI CCGC 3 cut(s) 9, 146, 234
SspMI CTAG 2 cut(s) 186, 575
StyD4I CCNGG 3 cut(s) 141, 220, 478
TaaI ACNGT 3 cut(s) 31, 454, 656
TaiI ACGT 2 cut(s) 40, 255
TaqI TCGA 3 cut(s) 90, 511, 531
TaqII GACCGA 2 cut(s) 78, 475
TasI AATT 4 cut(s) 393, 472, 533, 632
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 2 cut(s) 14, 269
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 2 cut(s) 457, 661
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 1 cut(s) 160
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 5 cut(s) 113, 282, 314, 481, 576
TspRI CASTG 2 cut(s) 457, 661
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 472, 533
XbaI TCTAGA 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 2 cut(s) 476, 512
XspI CTAG 2 cut(s) 186, 575
ZraI GACGTC 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.