Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014443

Physical Location & Seq

Genomic Coordinates Forward (+)

21340425 .. 21342406

Gene Structure 1982 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1252800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGCAAAATCTTTCTCGCAAATTCTCAACAGTCCCAATTCACAAGCTTCTACCTTCCCAGCTTCACCAGCACAGACCACATCCGGCCGAAATCTTCTCGGAGTTTCAGCAGAGGCGGAAGCTCTTGGAGCACAAACTCGTCGCCGTTCTAGATGGATGCGCCGGCCTCCGCCAAGTCAAAGCGGTTCACGGTCACCTCCTCCTCCACGGCCTCGGCCAATGCTGCTACGTTCTCACCAAGCTCATCCGCACGCTCACGAAGCTCGACGTCCCCATGGACGCGTATCCGCTCCTAGTCTTCCGACAGATCAAGTACCCAAACCCGTTTCTCTGGACGGCTATGATCCGCGGGTACAGTATCCAAGGACCTGTTTCGGAATCGCTGAATTTTTATACTTGTATGAGGAAATCGGGTGTTGGGCCGGTGTCGTTTACGTTCTCGGCGCTTTTTAAGGCCTGTGGGGATGTGCTTGATGTGAATTTGGGGCGTCAGATTCATGCCCACACGATTTTAGTTGGTGGGTTCGTGGCGGATTTGTATGTGGGGAACACTATGATCGATATGTATGTGAAATGTGGGTTTTTGGAGTGTGGGCGCAAGGTGTTTGATGAAATGCCTGAGAGGGATGTTGTTTCTTGGACTGAGTTGATTGTTGCTTATACCAAGGTTGGGGATATGGAATCAGCTCGGGAGTTGTTTGAGGGGTTGCCTGCAAAAGATATGGTGGCGTGGACTGCAATGGTTAACGGTTATGCTCAGAATGCCCATCCGAGAGAGGCGTTGGATTGCTTTGAGAGAATGCAGTGTGCTGGTGTAGGAACTGATGAGATTACATTGGCTGGTCTCATTTCGGCTTGTGCACAATTAGGTGCGTCTAAATATGCTAATTGGGTTCGGGACATTGCTGAGAAATCCGGTTTTGGGCCTACTGAAAATGTTCTTGTGGGTTCTGCATTGATAGATATGTACTCCAAGTGTGGAAGCTTGGATAATGCATTCAAGGTTTTTCAGGGAATGAAGGAAAGAAACGTATTTTCTTATAGCTCAATGATTTTGGGGTTTGCAATGCACGGTCGTGCAAGTGCAGCAATAGAATTATTTCATGAGATGTTGACGACCGAGATAAGACCTAACAAGGTTACTTTTATAGGGGTGCTGACAGCTTGCAGCCATGCTGGTATGGTTGATCAAGGTCGGCAGTTATTTGCAACCATGGAGAAGTACTATGATGTTGTTCCAACAGCTGATCATTACACTTGCATGGTAGACCTACTTGGTCGAGCTGGGCGCTTGGAAGAAGCTCTTGACCTTGTTGAAACAATGCCAATAGATGCCCATGGAGGCGTGTGGGGAGCTCTACTAGGGGCATGTCGTACCCATGGGAATCCCGACATTGCTCAGATTGCTGCTAACCATCTTTTTGAGCTTGAACCTGATAGCATTGGAAACTATGTAGTGCTCTCGAACATTTATGCATCAGCAGGAAGGTGGGATGAGGTTTCAAGAGTGAGGAAGTTGATGAGGGAAAAGGGTCTGAAAAAGAAACCTGCATATAGTTGGGTTGAAACAAAGAAAGGTGTAATTCACGAGTTCTGTGCAGGTGAAACAACCCATCCAGAGTACACCGAGATTAAGAAGACGTTGGAGGACCTTCTGGACAGATTACTGGCTAATGGCTACCAGCCTAACCTGGATTCCGCGGCTTATGCTGTGGGAGATGATGAAAAGACGCGTATATTAATGTCTCATAGTGAAAAGCTAGCTTTAGCGTATGCATTGCTCAGTACAGATTCTGTTTCCACCATTAAGATTATGAAGAACATTAGGATTTGTGAAGACTGTCATTCGTTTATGTGTGGTGCATCCCAAGTTACGGGGAGGGAGATTGTCGTGAGAGATAACATGAGATTCCACCACTTCCACAACGGGACATGCTCTTGCGGAAACTTTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

652

Amino Acids

72.49

Weight (kDa)

6.68

Isoelectric Point (pI)

32.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	106 - 153	2.5e-06	PPR repeat family
PPR_2	PF13041	240 - 287	3.3e-08	PPR repeat family
PPR	PF01535	242 - 272	3.5e-06	PPR repeat
PPR_2	PF13041	342 - 390	9.2e-09	PPR repeat family
E_motif	PF20431	460 - 522	1.9e-21	E motif
DYW_deaminase	PF14432	559 - 651	1.5e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44230 AT5G44230
fragaria_vesca	FvH4_1g12540
malus_domestica	MD15G1252800.v1.1
prunus_persica	Prupe.7G161300_v2.0.a1
pyrus_communis	pycom15g22270
rosa_chinensis	RchiOBHm_Chr2g0100491
rosa_laevigata	RLG00000016976
rosa_multiflora	Rmu_sc0000528.1_g000023 Rmu_sc0020397.1_g000001
rosa_roxburghii	Rroxscaffold_2G00142190
rosa_rugosa	Rorug02G0088600
rosa_samantha	Rh2AG138300 Rh2BG141300 Rh2CG143500 Rh2DG143100
rosa_wichuraiana	Rw2G010810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1589
AatII	GACGTC	1 cut(s)	270
Acc36I	ACCTGC	2 cut(s)	1555, 1589
AccBSI	CCGCTC	1 cut(s)	289
AccI	GTMKAC	1 cut(s)	1266
AccII	CGCG	4 cut(s)	281, 348, 1700, 1732
AclWI	GGATC	1 cut(s)	337
AcoI	YGGCCR	2 cut(s)	84, 214
AcsI	RAATTY	3 cut(s)	20, 385, 478
AcyI	GRCGYC	2 cut(s)	267, 487
AfaI	GTAC	7 cut(s)	314, 353, 968, 1223, 1375, 1622, 1786
AfiI	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
AflIII	ACRYGT	2 cut(s)	279, 1730
AgsI	TTSAA	5 cut(s)	1000, 1316, 1430, 1503, 1565
AjnI	CCWGG	1 cut(s)	1689
AleI	CACNNNNGTG	1 cut(s)	1074
Alw21I	GWGCWC	4 cut(s)	132, 862, 1357, 1461
Alw26I	GTCTC	2 cut(s)	848, 1749
Alw44I	GTGCAC	1 cut(s)	858
AlwI	GGATC	1 cut(s)	337
AlwNI	CAGNNNCTG	1 cut(s)	1793
Ama87I	CYCGRG	1 cut(s)	687
AoxI	GGCC	7 cut(s)	84, 163, 208, 214, 419, 453, 923
ApaLI	GTGCAC	1 cut(s)	858
ApeKI	GCWGC	4 cut(s)	222, 1085, 1167, 1406
ApoI	RAATTY	3 cut(s)	20, 385, 478
AseI	ATTAAT	1 cut(s)	1739
Asp700I	GAANNNNTTC	2 cut(s)	936, 1098
AspLEI	GCGC	4 cut(s)	161, 445, 597, 1290
AspS9I	GGNCC	4 cut(s)	365, 419, 923, 1648
AsuHPI	GGTGA	4 cut(s)	56, 185, 226, 1613
AsuNHI	GCTAGC	1 cut(s)	1759
AvaI	CYCGRG	1 cut(s)	687
AvaII	GGWCC	2 cut(s)	365, 1648
BaeGI	GKGCMC	1 cut(s)	862
BanII	GRGCYC	1 cut(s)	1357
BauI	CACGAG	1 cut(s)	1586
BbsI	GAAGAC	3 cut(s)	289, 1643, 1842
Bbv12I	GWGCWC	4 cut(s)	132, 862, 1357, 1461
BbvI	GCAGC	4 cut(s)	209, 1097, 1179, 1393
BccI	CCATC	4 cut(s)	146, 774, 1422, 1620
BceAI	ACGGC	3 cut(s)	128, 223, 351
BcgI	CGANNNNNNTGC	2 cut(s)	1577, 1611
BciT130I	CCWGG	1 cut(s)	1691
BciVI	GTATCC	2 cut(s)	294, 368
BclI	TGATCA	2 cut(s)	1186, 1246
BcoDI	GTCTC	2 cut(s)	848, 1749
BfaI	CTAG	4 cut(s)	149, 293, 1361, 1760
BfoI	RGCGCY	2 cut(s)	446, 1291
BfuAI	ACCTGC	2 cut(s)	1555, 1589
BfuI	GTATCC	2 cut(s)	294, 368
BisI	GCNGC	5 cut(s)	223, 1086, 1168, 1407, 1701
BlsI	GCNGC	5 cut(s)	224, 1087, 1169, 1408, 1702
BmcAI	AGTACT	1 cut(s)	1223
Bme1390I	CCNGG	1 cut(s)	1691
Bme18I	GGWCC	2 cut(s)	365, 1648
BmeT110I	CYCGRG	1 cut(s)	687
BmgT120I	GGNCC	4 cut(s)	365, 419, 923, 1648
BmrFI	CCNGG	1 cut(s)	1691
BmsI	GCATC	4 cut(s)	146, 1321, 1484, 1871
BmtI	GCTAGC	1 cut(s)	1763
BpiI	GAAGAC	3 cut(s)	289, 1643, 1842
Bsa29I	ATCGAT	1 cut(s)	558
BsaHI	GRCGYC	2 cut(s)	267, 487
BsaI	GGTCTC	1 cut(s)	848
BsaWI	WCCGGW	1 cut(s)	914
Bsc4I	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
Bse118I	RCCGGY	2 cut(s)	161, 421
Bse1I	ACTGG	1 cut(s)	1671
Bse3DI	GCAATG	5 cut(s)	744, 900, 1071, 1392, 1775
BseBI	CCWGG	1 cut(s)	1691
BseCI	ATCGAT	1 cut(s)	558
BseGI	GGATG	9 cut(s)	79, 161, 243, 469, 631, 766, 1498, 1612, 1862
BseLI	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
BseMI	GCAATG	5 cut(s)	744, 900, 1071, 1392, 1775
BseMII	CTCAG	6 cut(s)	609, 633, 770, 897, 1412, 1795
BseNI	ACTGG	1 cut(s)	1671
BseRI	GAGGAG	2 cut(s)	188, 191
BseSI	GKGCMC	1 cut(s)	862
BseX3I	CGGCCG	1 cut(s)	84
BseXI	GCAGC	4 cut(s)	209, 1097, 1179, 1393
BseYI	CCCAGC	2 cut(s)	57, 1283
BsgI	GTGCAG	2 cut(s)	1104, 1617
Bsh1236I	CGCG	4 cut(s)	281, 348, 1700, 1732
Bsh1285I	CGRYCG	3 cut(s)	87, 1075, 1119
BshFI	GGCC	7 cut(s)	86, 165, 210, 216, 421, 455, 925
BshVI	ATCGAT	1 cut(s)	558
BsiEI	CGRYCG	3 cut(s)	87, 1075, 1119
BsiHKAI	GWGCWC	4 cut(s)	132, 862, 1357, 1461
BsiHKCI	CYCGRG	1 cut(s)	687
BsiSI	CCGG	4 cut(s)	83, 162, 422, 915
BslFI	GGGAC	4 cut(s)	17, 254, 911, 1942
BslI	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
BsmAI	GTCTC	2 cut(s)	848, 1749
BsmFI	GGGAC	4 cut(s)	17, 254, 911, 1942
BsmI	GAATGC	3 cut(s)	766, 804, 995
BsnI	GGCC	7 cut(s)	86, 165, 210, 216, 421, 455, 925
Bso31I	GGTCTC	1 cut(s)	848
BsoBI	CYCGRG	1 cut(s)	687
Bsp1286I	GDGCHC	4 cut(s)	132, 862, 1357, 1461
Bsp143I	GATC	5 cut(s)	306, 342, 555, 1186, 1246
Bsp19I	CCATGG	4 cut(s)	273, 1212, 1336, 1378
BspANI	GGCC	7 cut(s)	86, 165, 210, 216, 421, 455, 925
BspCNI	CTCAG	6 cut(s)	610, 634, 769, 898, 1411, 1794
BspDI	ATCGAT	1 cut(s)	558
BspFNI	CGCG	4 cut(s)	281, 348, 1700, 1732
BspHI	TCATGA	1 cut(s)	1102
BspMI	ACCTGC	2 cut(s)	1555, 1589
BspOI	GCTAGC	1 cut(s)	1763
BspPI	GGATC	1 cut(s)	337
BspTNI	GGTCTC	1 cut(s)	848
BsrBI	CCGCTC	1 cut(s)	289
BsrDI	GCAATG	5 cut(s)	744, 900, 1071, 1392, 1775
BsrFI	RCCGGY	2 cut(s)	161, 421
BsrI	ACTGG	1 cut(s)	1671
BssAI	RCCGGY	2 cut(s)	161, 421
BssMI	GATC	5 cut(s)	306, 342, 555, 1186, 1246
BssNI	GRCGYC	2 cut(s)	267, 487
BssSI	CACGAG	1 cut(s)	1586
BssT1I	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
Bst2BI	CACGAG	1 cut(s)	1586
Bst2UI	CCWGG	1 cut(s)	1691
Bst4CI	ACNGT	6 cut(s)	31, 191, 356, 749, 1073, 1841
BstACI	GRCGYC	2 cut(s)	267, 487
BstC8I	GCNNGC	5 cut(s)	163, 251, 711, 1165, 1761
BstDEI	CTNAG	6 cut(s)	618, 642, 756, 906, 1398, 1781
BstDSI	CCRYGG	7 cut(s)	205, 273, 346, 1212, 1336, 1378, 1698
BstEII	GGTNACC	1 cut(s)	191
BstF5I	GGATG	9 cut(s)	79, 161, 243, 469, 631, 766, 1498, 1612, 1862
BstFNI	CGCG	4 cut(s)	281, 348, 1700, 1732
BstH2I	RGCGCY	2 cut(s)	446, 1291
BstHHI	GCGC	4 cut(s)	161, 445, 597, 1290
BstKTI	GATC	5 cut(s)	309, 345, 558, 1189, 1249
BstMAI	GTCTC	2 cut(s)	848, 1749
BstMBI	GATC	5 cut(s)	306, 342, 555, 1186, 1246
BstMCI	CGRYCG	3 cut(s)	87, 1075, 1119
BstMWI	GCNNNNNNNGC	9 cut(s)	67, 127, 222, 259, 734, 761, 1085, 1403, 1706
BstNI	CCWGG	1 cut(s)	1691
BstNSI	RCATGY	2 cut(s)	1371, 1935
BstPI	GGTNACC	1 cut(s)	191
BstSCI	CCNGG	1 cut(s)	1689
BstSLI	GKGCMC	1 cut(s)	862
BstUI	CGCG	4 cut(s)	281, 348, 1700, 1732
BstV1I	GCAGC	4 cut(s)	209, 1097, 1179, 1393
BstV2I	GAAGAC	3 cut(s)	289, 1643, 1842
BstZI	CGGCCG	1 cut(s)	84
Bsu15I	ATCGAT	1 cut(s)	558
BsuI	GTATCC	2 cut(s)	294, 368
BsuRI	GGCC	7 cut(s)	86, 165, 210, 216, 421, 455, 925
BsuTUI	ATCGAT	1 cut(s)	558
BtgI	CCRYGG	7 cut(s)	205, 273, 346, 1212, 1336, 1378, 1698
BtsCI	GGATG	9 cut(s)	79, 161, 243, 469, 631, 766, 1498, 1612, 1862
BtsI	GCAGTG	1 cut(s)	809
BtsIMutI	CAGTG	1 cut(s)	809
BveI	ACCTGC	2 cut(s)	1555, 1589
Cac8I	GCNNGC	5 cut(s)	163, 251, 711, 1165, 1761
CaiI	CAGNNNCTG	1 cut(s)	1793
CciI	TCATGA	1 cut(s)	1102
CfoI	GCGC	4 cut(s)	161, 445, 597, 1290
Cfr10I	RCCGGY	2 cut(s)	161, 421
Cfr13I	GGNCC	4 cut(s)	365, 419, 923, 1648
Cfr42I	CCGCGG	2 cut(s)	349, 1701
ClaI	ATCGAT	1 cut(s)	558
CseI	GACGC	4 cut(s)	287, 476, 861, 1738
Csp6I	GTAC	7 cut(s)	313, 352, 967, 1222, 1374, 1621, 1785
CviQI	GTAC	7 cut(s)	313, 352, 967, 1222, 1374, 1621, 1785
DdeI	CTNAG	6 cut(s)	618, 642, 756, 906, 1398, 1781
DpnI	GATC	5 cut(s)	308, 344, 557, 1188, 1248
DpnII	GATC	5 cut(s)	306, 342, 555, 1186, 1246
EaeI	YGGCCR	2 cut(s)	84, 214
EagI	CGGCCG	1 cut(s)	84
EciI	GGCGGA	3 cut(s)	130, 158, 545
Ecl136II	GAGCTC	1 cut(s)	1355
EclXI	CGGCCG	1 cut(s)	84
Eco130I	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
Eco147I	AGGCCT	1 cut(s)	455
Eco24I	GRGCYC	1 cut(s)	1357
Eco31I	GGTCTC	1 cut(s)	848
Eco47I	GGWCC	2 cut(s)	365, 1648
Eco52I	CGGCCG	1 cut(s)	84
Eco53kI	GAGCTC	1 cut(s)	1355
Eco88I	CYCGRG	1 cut(s)	687
Eco91I	GGTNACC	1 cut(s)	191
EcoICRI	GAGCTC	1 cut(s)	1355
EcoO109I	RGGNCCY	2 cut(s)	365, 1648
EcoO65I	GGTNACC	1 cut(s)	191
EcoRII	CCWGG	1 cut(s)	1689
EcoT14I	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
EcoT22I	ATGCAT	3 cut(s)	997, 1477, 1777
EcoT38I	GRGCYC	1 cut(s)	1357
ErhI	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
FalI	AAGNNNNNCTT	2 cut(s)	1287, 1319
FaqI	GGGAC	4 cut(s)	17, 254, 911, 1942
FauI	CCCGC	1 cut(s)	341
FbaI	TGATCA	2 cut(s)	1186, 1246
FblI	GTMKAC	1 cut(s)	1266
Fnu4HI	GCNGC	5 cut(s)	223, 1086, 1168, 1407, 1701
FokI	GGATG	9 cut(s)	66, 168, 230, 476, 638, 753, 1505, 1599, 1849
FriOI	GRGCYC	1 cut(s)	1357
Fsp4HI	GCNGC	5 cut(s)	223, 1086, 1168, 1407, 1701
FspBI	CTAG	4 cut(s)	149, 293, 1361, 1760
GlaI	GCGC	4 cut(s)	160, 444, 596, 1289
GluI	GCNGC	5 cut(s)	223, 1086, 1168, 1407, 1701
GsaI	CCCAGC	2 cut(s)	61, 1287
HaeII	RGCGCY	2 cut(s)	446, 1291
HaeIII	GGCC	7 cut(s)	86, 165, 210, 216, 421, 455, 925
HapII	CCGG	4 cut(s)	83, 162, 422, 915
HgaI	GACGC	4 cut(s)	287, 476, 861, 1738
HhaI	GCGC	4 cut(s)	161, 445, 597, 1290
Hin1I	GRCGYC	2 cut(s)	267, 487
Hin6I	GCGC	4 cut(s)	159, 443, 595, 1288
HinP1I	GCGC	4 cut(s)	159, 443, 595, 1288
HincII	GTYRAC	2 cut(s)	745, 1113
HindII	GTYRAC	2 cut(s)	745, 1113
HindIII	AAGCTT	2 cut(s)	44, 982
HinfI	GANTC	7 cut(s)	377, 493, 680, 1384, 1694, 1790, 1908
HpaI	GTTAAC	1 cut(s)	745
HpaII	CCGG	4 cut(s)	83, 162, 422, 915
HphI	GGTGA	4 cut(s)	56, 185, 226, 1613
Hpy166II	GTNNAC	8 cut(s)	187, 432, 732, 745, 860, 1113, 1267, 1623
Hpy188I	TCNGA	8 cut(s)	100, 302, 376, 492, 759, 771, 1401, 1536
Hpy8I	GTNNAC	8 cut(s)	187, 432, 732, 745, 860, 1113, 1267, 1623
Hpy99I	CGWCG	2 cut(s)	143, 269
HpyAV	CCTTC	4 cut(s)	63, 1012, 1479, 1661
HpyCH4III	ACNGT	6 cut(s)	31, 191, 356, 749, 1073, 1841
HpyCH4IV	ACGT	5 cut(s)	228, 267, 434, 1029, 1640
HpyF10VI	GCNNNNNNNGC	9 cut(s)	67, 127, 222, 259, 734, 761, 1085, 1403, 1706
HpyF3I	CTNAG	6 cut(s)	618, 642, 756, 906, 1398, 1781
HpySE526I	ACGT	5 cut(s)	228, 267, 434, 1029, 1640
Hsp92I	GRCGYC	2 cut(s)	267, 487
HspAI	GCGC	4 cut(s)	159, 443, 595, 1288
KroI	GCCGGC	1 cut(s)	161
KroNI	GCCGGC	1 cut(s)	163
Ksp22I	TGATCA	2 cut(s)	1186, 1246
KspAI	GTTAAC	1 cut(s)	745
KspI	CCGCGG	2 cut(s)	349, 1701
Kzo9I	GATC	5 cut(s)	306, 342, 555, 1186, 1246
LmnI	GCTCC	3 cut(s)	127, 294, 1352
Lsp1109I	GCAGC	4 cut(s)	209, 1097, 1179, 1393
LweI	GCATC	4 cut(s)	146, 1321, 1484, 1871
MaeI	CTAG	4 cut(s)	149, 293, 1361, 1760
MaeII	ACGT	5 cut(s)	228, 267, 434, 1029, 1640
MaeIII	GTNAC	3 cut(s)	191, 1138, 1870
MalI	GATC	5 cut(s)	308, 344, 557, 1188, 1248
MbiI	CCGCTC	1 cut(s)	289
MboI	GATC	5 cut(s)	306, 342, 555, 1186, 1246
MboII	GAAGA	6 cut(s)	85, 289, 1307, 1648, 1828, 1847
MhlI	GDGCHC	4 cut(s)	132, 862, 1357, 1461
MluCI	AATT	8 cut(s)	20, 36, 385, 478, 863, 886, 1094, 1581
MluI	ACGCGT	2 cut(s)	279, 1730
MmeI	TCCRAC	4 cut(s)	325, 762, 1262, 1623
Mph1103I	ATGCAT	3 cut(s)	997, 1477, 1777
MroNI	GCCGGC	1 cut(s)	161
MroXI	GAANNNNTTC	2 cut(s)	936, 1098
MseI	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
MslI	CAYNNNNRTG	2 cut(s)	1074, 1259
MspA1I	CMGCKG	3 cut(s)	348, 1244, 1700
MspI	CCGG	4 cut(s)	83, 162, 422, 915
MspR9I	CCNGG	1 cut(s)	1691
Mva1269I	GAATGC	3 cut(s)	766, 804, 995
MvaI	CCWGG	1 cut(s)	1691
MvnI	CGCG	4 cut(s)	281, 348, 1700, 1732
MwoI	GCNNNNNNNGC	9 cut(s)	67, 127, 222, 259, 734, 761, 1085, 1403, 1706
NaeI	GCCGGC	1 cut(s)	163
NcoI	CCATGG	4 cut(s)	273, 1212, 1336, 1378
NdeII	GATC	5 cut(s)	306, 342, 555, 1186, 1246
NgoMIV	GCCGGC	1 cut(s)	161
NheI	GCTAGC	1 cut(s)	1759
NmeAIII	GCCGAG	2 cut(s)	192, 419
NmuCI	GTSAC	1 cut(s)	191
NsiI	ATGCAT	3 cut(s)	997, 1477, 1777
NspI	RCATGY	2 cut(s)	1371, 1935
OliI	CACNNNNGTG	1 cut(s)	1074
PagI	TCATGA	1 cut(s)	1102
PaqCI	CACCTGC	1 cut(s)	1589
PceI	AGGCCT	1 cut(s)	455
PctI	GAATGC	3 cut(s)	766, 804, 995
PdiI	GCCGGC	1 cut(s)	163
PdmI	GAANNNNTTC	2 cut(s)	936, 1098
PfeI	GAWTC	7 cut(s)	377, 493, 680, 1384, 1694, 1790, 1908
PkrI	GCNGC	5 cut(s)	224, 1087, 1169, 1408, 1702
PpuMI	RGGWCCY	2 cut(s)	365, 1648
PshBI	ATTAAT	1 cut(s)	1739
Psp124BI	GAGCTC	1 cut(s)	1357
Psp5II	RGGWCCY	2 cut(s)	365, 1648
Psp6I	CCWGG	1 cut(s)	1689
PspEI	GGTNACC	1 cut(s)	191
PspFI	CCCAGC	2 cut(s)	57, 1283
PspGI	CCWGG	1 cut(s)	1689
PspPI	GGNCC	4 cut(s)	365, 419, 923, 1648
PspPPI	RGGWCCY	2 cut(s)	365, 1648
PstNI	CAGNNNCTG	1 cut(s)	1793
PvuII	CAGCTG	1 cut(s)	1244
RsaI	GTAC	7 cut(s)	314, 353, 968, 1223, 1375, 1622, 1786
RsaNI	GTAC	7 cut(s)	313, 352, 967, 1222, 1374, 1621, 1785
RseI	CAYNNNNRTG	2 cut(s)	1074, 1259
SacI	GAGCTC	1 cut(s)	1357
SacII	CCGCGG	2 cut(s)	349, 1701
SaqAI	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
SatI	GCNGC	5 cut(s)	223, 1086, 1168, 1407, 1701
Sau3AI	GATC	5 cut(s)	306, 342, 555, 1186, 1246
Sau96I	GGNCC	4 cut(s)	365, 419, 923, 1648
ScaI	AGTACT	1 cut(s)	1223
ScrFI	CCNGG	1 cut(s)	1691
SduI	GDGCHC	4 cut(s)	132, 862, 1357, 1461
SfaNI	GCATC	4 cut(s)	146, 1321, 1484, 1871
Sfr303I	CCGCGG	2 cut(s)	349, 1701
SgrBI	CCGCGG	2 cut(s)	349, 1701
SinI	GGWCC	2 cut(s)	365, 1648
SmiMI	CAYNNNNRTG	2 cut(s)	1074, 1259
Sse9I	AATT	8 cut(s)	20, 36, 385, 478, 863, 886, 1094, 1581
SseBI	AGGCCT	1 cut(s)	455
SspMI	CTAG	4 cut(s)	149, 293, 1361, 1760
SstI	GAGCTC	1 cut(s)	1357
StuI	AGGCCT	1 cut(s)	455
StyD4I	CCNGG	1 cut(s)	1689
StyI	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
TaaI	ACNGT	6 cut(s)	31, 191, 356, 749, 1073, 1841
TaiI	ACGT	5 cut(s)	231, 270, 437, 1032, 1643
TaqI	TCGA	4 cut(s)	264, 558, 1279, 1463
TaqII	GACCGA	1 cut(s)	1133
TasI	AATT	8 cut(s)	20, 36, 385, 478, 863, 886, 1094, 1581
TatI	WGTACW	4 cut(s)	966, 1221, 1620, 1784
TauI	GCSGC	1 cut(s)	1703
TfiI	GAWTC	7 cut(s)	377, 493, 680, 1384, 1694, 1790, 1908
Tru1I	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
Tru9I	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
TscAI	CASTG	1 cut(s)	809
TseFI	GTSAC	1 cut(s)	191
TseI	GCWGC	4 cut(s)	222, 1085, 1167, 1406
Tsp45I	GTSAC	1 cut(s)	191
TspDTI	ATGAA	6 cut(s)	485, 624, 1031, 1091, 1737, 1829
TspRI	CASTG	1 cut(s)	809
VneI	GTGCAC	1 cut(s)	858
VpaK11BI	GGWCC	2 cut(s)	365, 1648
VspI	ATTAAT	1 cut(s)	1739
XapI	RAATTY	3 cut(s)	20, 385, 478
XbaI	TCTAGA	1 cut(s)	148
XceI	RCATGY	2 cut(s)	1371, 1935
XmiI	GTMKAC	1 cut(s)	1266
XmnI	GAANNNNTTC	2 cut(s)	936, 1098
XspI	CTAG	4 cut(s)	149, 293, 1361, 1760
ZraI	GACGTC	1 cut(s)	268
ZrmI	AGTACT	1 cut(s)	1223
Zsp2I	ATGCAT	3 cut(s)	997, 1477, 1777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.