Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0014443

Physical Location & Seq

Genomic Coordinates Forward (+)

12427172 .. 12429519

Gene Structure 2348 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ47512

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1950 bp

ATGCAAAACCTCTCTCGCAAATTCTCAACGCTCCCAATTCCCAAGCTTTCACCTTCACACCCACAACCCACCACACCCTTCACCCCATACTCTGAGATTCAGCATTGCCGGAAAATCTTAGAGCACAAAATCATTACTATTCTAGACGCGTGCAACACCCTCAACCATGTCAAGCAGGTTCACGCTCACGTCATCGGAAATGGGCTCAGCCAATACAGCTACGTCATCACCAAGCTCGTCCGCACGCTCACCAAGCTCGGAGTCCCAATGCCCACCACCTACCCGGAACTCGTCTTCCGGCAGGTGCTGCACCCGAACCCGTTTCTCTGGGCGGCTATGATTAGAGGGTGTGCAATTGAAGGAACTCTTTCGGAAGCGGTGAAGTATTACACTTTGATGAGGAGGGAGGGCACCGGCCCGGTTTCGTTTACGTTCTCGGCGCTGTTCAAGGCCTGTGGGAGTGGTCTGGATGTGAATTTGGGCCGGCAGATACATGCGCAGACGGTTTTGATCGGTGGGTTTGTGGCGGATTTGTACGTGGGGAACACTCTGATTGATATGTATTTGAAGTGTGGGTTTTTGGATTGCGGGCGCAAGGTGTTTGATGAAATGCCTGAGAGGGATGTGGTTTCGTGGACCGAGTTGATTGTGGCGTATGCCAAGAGTGGGGATATGGAGTCGGCGAGGGACTTGTTTCAGGATTTGAGTTTGAAAGATATGGTGGTGTGGACGGCTATGGTTACTGGTTATGCCCAGAATTCGATGCCGAAAGAGGCCTTGGATTGCTTTGAGAGAATGCGAGGTGCTGGTGTAGGGCTTGATGAGGTTACATTGCTCGGGGTCATTTCGGCTTGTGCACAATTGGGTGCTTCCAGGTATGCTAATTGGGTTCGAGACATTGCTGAGGAATCTGGTTTTGGGGCAACTCAGAATGTGCTTGTGGGATCTGCATTGATAGATATGTTCTCCAAGTGTGGGAGCTTGGATGATGCATACAATATTTTTCAGGGGATGAAGCAAAGGAATGTGTTTTCTTATAGCTCAATGATTTGGGGATTTGCAGTGCATGGAAATGCCAATGCAGCGATAGGATTGTTTCATGAGATGCTGACCACTGATGTAAGGCCGAACTGGGTTACTTTTATAGGTGTGCTAACTGCCTGCAGCCACGCGGGGATGGTTGATCAAGGTCGGCAGCTATTTGCAACGATGGAGAAGTATTACAATGTCACTCCATCGGCTGGTCATTACACTTGTATGGTAGACTTGCTTGGTCGAGCTGGGCGCTTGGAAGAAGCTCTCGAGCTAGCTGAGACAATGCCGATAGTCGCACATGGAGGTGTGTGGGGAGCACTACTTGGTGCGTGCCGTATCCATAAGAACCCCGACATTGCTCAAATTGCTGCCAGCCATCTATTTGAGCTTGAACCTAATAATATTGGAAACTATGTGATGCTCTCCAACATATATGCATCAGCAGGAAGGTGGGATGATGTTTCAAGTGTTAGGAAGCTGATGAGAGAAAAGGGTCTGAAAAAAAACCCTGCATATAGCTGGGTTGAAACAAAGAACGGACACGAAAAGTTTTGTGCAGGTGAAACGAGTCACCCCAAATACAACAAGATTAAGAAGGCGTTGGAGGATCTTCTAAACAGATTGCAGGATCGTGGCTATAGGCCAAACCTAAATTCGGTGGATTATGATGTGGGGGATGAAGAAAAGATGCGTATATTGATGGCTCATAGCGAAAAACTGGCTTTGGCATACGCATTGCTCTACGCAGATTCTGGTTCCACCATTAAGATCGCCAAGAACATAAGGATTTGTGAAGACTGTCATGCCTTCATGTGTGGTGCATCACGGGTTACAGGAAGGCATATTATTGTCAGAGATAACATGAGATTCCACCACTTCGAAAATGGGACTTGTAATTGCGGCAACTTTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

649

Amino Acids

72.19

Weight (kDa)

7.55

Isoelectric Point (pI)

31.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	239 - 286	9.7e-07	PPR repeat family
PPR	PF01535	241 - 271	3.5e-07	PPR repeat
PPR_2	PF13041	341 - 389	1.9e-08	PPR repeat family
E_motif	PF20431	459 - 521	1.3e-21	E motif
DYW_deaminase	PF14432	557 - 649	1.7e-40	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44230 AT5G44230
fragaria_vesca	FvH4_1g12540
malus_domestica	MD15G1252800.v1.1
prunus_persica	Prupe.7G161300_v2.0.a1
pyrus_communis	pycom15g22270
rosa_chinensis	RchiOBHm_Chr2g0100491
rosa_laevigata	RLG00000016976
rosa_multiflora	Rmu_sc0000528.1_g000023 Rmu_sc0020397.1_g000001
rosa_roxburghii	Rroxscaffold_2G00142190
rosa_rugosa	Rorug02G0088600
rosa_samantha	Rh2AG138300 Rh2BG141300 Rh2CG143500 Rh2DG143100
rosa_wichuraiana	Rw2G010810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	292, 1583
Acc16I	TGCGCA	1 cut(s)	498
Acc36I	ACCTGC	3 cut(s)	166, 292, 1583
AccB1I	GGYRCC	1 cut(s)	410
AccB7I	CCANNNNNTGG	1 cut(s)	1241
AccI	GTMKAC	1 cut(s)	1263
AccII	CGCG	2 cut(s)	149, 1172
AciI	CCGC	7 cut(s)	241, 332, 377, 527, 588, 1172, 1935
AclWI	GGATC	3 cut(s)	952, 1650, 1671
AcsI	RAATTY	4 cut(s)	20, 475, 757, 1687
AfaI	GTAC	1 cut(s)	536
AfiI	CCNNNNNNNGG	4 cut(s)	666, 975, 1241, 1690
AflIII	ACRYGT	1 cut(s)	147
AgsI	TTSAA	7 cut(s)	359, 448, 568, 712, 1427, 1500, 1562
AjiI	CACGTC	1 cut(s)	190
AjnI	CCWGG	1 cut(s)	872
AjuI	GAANNNNNNNTTGG	6 cut(s)	761, 793, 900, 932, 1672, 1704
AloI	GAACNNNNNNTCC	2 cut(s)	279, 311
Alw21I	GWGCWC	3 cut(s)	126, 859, 1354
Alw26I	GTCTC	2 cut(s)	888, 1307
Alw44I	GTGCAC	1 cut(s)	855
AlwI	GGATC	3 cut(s)	952, 1650, 1671
AlwNI	CAGNNNCTG	2 cut(s)	307, 1787
Ama87I	CYCGRG	2 cut(s)	836, 1301
AoxI	GGCC	6 cut(s)	415, 450, 481, 774, 1124, 1676
ApaLI	GTGCAC	1 cut(s)	855
ApeKI	GCWGC	5 cut(s)	307, 1082, 1164, 1195, 1403
ApoI	RAATTY	4 cut(s)	20, 475, 757, 1687
Asp700I	GAANNNNTTC	1 cut(s)	367
AspLEI	GCGC	4 cut(s)	442, 499, 594, 1287
AspS9I	GGNCC	3 cut(s)	416, 481, 636
AsuC2I	CCSGG	2 cut(s)	284, 419
AsuHPI	GGTGA	7 cut(s)	42, 73, 220, 241, 391, 1598, 1607
AsuII	TTCGAA	1 cut(s)	1914
AsuNHI	GCTAGC	1 cut(s)	1306
AvaI	CYCGRG	2 cut(s)	836, 1301
AvaII	GGWCC	1 cut(s)	636
BaeGI	GKGCMC	2 cut(s)	413, 859
BanI	GGYRCC	1 cut(s)	410
BanII	GRGCYC	1 cut(s)	207
BbsI	GAAGAC	2 cut(s)	286, 1836
Bbv12I	GWGCWC	3 cut(s)	126, 859, 1354
BbvCI	CCTCAGC	1 cut(s)	903
BbvI	GCAGC	5 cut(s)	294, 1094, 1176, 1207, 1390
BccI	CCATC	5 cut(s)	1171, 1204, 1243, 1419, 1729
BceAI	ACGGC	2 cut(s)	747, 1353
BciT130I	CCWGG	1 cut(s)	874
BciVI	GTATCC	1 cut(s)	1382
BclI	TGATCA	1 cut(s)	1183
BcnI	CCSGG	2 cut(s)	284, 419
BcoDI	GTCTC	2 cut(s)	888, 1307
BfaI	CTAG	2 cut(s)	143, 1307
BfmI	CTRYAG	2 cut(s)	1162, 1672
BfoI	RGCGCY	2 cut(s)	443, 1288
BfuAI	ACCTGC	3 cut(s)	166, 292, 1583
BfuI	GTATCC	1 cut(s)	1382
BisI	GCNGC	7 cut(s)	308, 333, 1083, 1165, 1196, 1404, 1936
BlpI	GCTNAGC	1 cut(s)	206
BlsI	GCNGC	7 cut(s)	309, 334, 1084, 1166, 1197, 1405, 1937
Bme1390I	CCNGG	3 cut(s)	284, 419, 874
Bme18I	GGWCC	1 cut(s)	636
BmeT110I	CYCGRG	2 cut(s)	836, 1301
BmgBI	CACGTC	1 cut(s)	190
BmgT120I	GGNCC	3 cut(s)	416, 481, 636
BmiI	GGNNCC	2 cut(s)	412, 1792
BmrFI	CCNGG	3 cut(s)	284, 419, 874
BmrI	ACTGGG	1 cut(s)	1141
BmsI	GCATC	7 cut(s)	753, 979, 1095, 1443, 1481, 1713, 1865
BmtI	GCTAGC	1 cut(s)	1310
BmuI	ACTGGG	1 cut(s)	1141
BpiI	GAAGAC	2 cut(s)	286, 1836
Bpu10I	CCTNAGC	1 cut(s)	903
Bpu1102I	GCTNAGC	1 cut(s)	206
Bpu14I	TTCGAA	1 cut(s)	1914
BpuMI	CCSGG	2 cut(s)	284, 419
BsaAI	YACGTR	1 cut(s)	538
BsaJI	CCNNGG	1 cut(s)	777
Bsc4I	CCNNNNNNNGG	4 cut(s)	666, 975, 1241, 1690
Bse118I	RCCGGY	2 cut(s)	413, 483
Bse1I	ACTGG	3 cut(s)	748, 1136, 1758
Bse3DI	GCAATG	5 cut(s)	103, 830, 897, 1389, 1769
BseBI	CCWGG	1 cut(s)	874
BseDI	CCNNGG	1 cut(s)	777
BseGI	GGATG	7 cut(s)	475, 628, 991, 1017, 1182, 1495, 1717
BseLI	CCNNNNNNNGG	4 cut(s)	666, 975, 1241, 1690
BseMI	GCAATG	5 cut(s)	103, 830, 897, 1389, 1769
BseMII	CTCAG	6 cut(s)	84, 220, 606, 894, 941, 1302
BseNI	ACTGG	3 cut(s)	748, 1136, 1758
BseRI	GAGGAG	1 cut(s)	415
BseSI	GKGCMC	2 cut(s)	413, 859
BseXI	GCAGC	5 cut(s)	294, 1094, 1176, 1207, 1390
BseYI	CCCAGC	2 cut(s)	1280, 1554
BsgI	GTGCAG	2 cut(s)	293, 1611
Bsh1236I	CGCG	2 cut(s)	149, 1172
BshFI	GGCC	6 cut(s)	417, 452, 483, 776, 1126, 1678
BshNI	GGYRCC	1 cut(s)	410
BsiHKAI	GWGCWC	3 cut(s)	126, 859, 1354
BsiHKCI	CYCGRG	2 cut(s)	836, 1301
BsiSI	CCGG	6 cut(s)	109, 284, 298, 414, 419, 484
BslFI	GGGAC	3 cut(s)	248, 701, 1936
BslI	CCNNNNNNNGG	4 cut(s)	666, 975, 1241, 1690
BsmAI	GTCTC	2 cut(s)	888, 1307
BsmFI	GGGAC	3 cut(s)	248, 701, 1936
BsmI	GAATGC	1 cut(s)	801
BsnI	GGCC	6 cut(s)	417, 452, 483, 776, 1126, 1678
BsoBI	CYCGRG	2 cut(s)	836, 1301
Bsp119I	TTCGAA	1 cut(s)	1914
Bsp1286I	GDGCHC	5 cut(s)	126, 207, 413, 859, 1354
Bsp143I	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
Bsp1720I	GCTNAGC	1 cut(s)	206
BspACI	CCGC	7 cut(s)	241, 332, 377, 527, 588, 1172, 1935
BspANI	GGCC	6 cut(s)	417, 452, 483, 776, 1126, 1678
BspCNI	CTCAG	6 cut(s)	85, 219, 607, 895, 940, 1303
BspFNI	CGCG	2 cut(s)	149, 1172
BspHI	TCATGA	1 cut(s)	1099
BspLI	GGNNCC	2 cut(s)	412, 1792
BspMAI	CTGCAG	1 cut(s)	1166
BspMI	ACCTGC	3 cut(s)	166, 292, 1583
BspOI	GCTAGC	1 cut(s)	1310
BspPI	GGATC	3 cut(s)	952, 1650, 1671
BspT104I	TTCGAA	1 cut(s)	1914
BspT107I	GGYRCC	1 cut(s)	410
BsrDI	GCAATG	5 cut(s)	103, 830, 897, 1389, 1769
BsrFI	RCCGGY	2 cut(s)	413, 483
BsrI	ACTGG	3 cut(s)	748, 1136, 1758
BssAI	RCCGGY	2 cut(s)	413, 483
BssECI	CCNNGG	1 cut(s)	777
BssMI	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
BssT1I	CCWWGG	1 cut(s)	777
Bst2UI	CCWGG	1 cut(s)	874
Bst4CI	ACNGT	2 cut(s)	505, 1835
BstAPI	GCANNNNNTGC	1 cut(s)	307
BstBAI	YACGTR	1 cut(s)	538
BstBI	TTCGAA	1 cut(s)	1914
BstC8I	GCNNGC	8 cut(s)	151, 245, 485, 590, 1162, 1308, 1366, 1408
BstDEI	CTNAG	7 cut(s)	93, 118, 206, 615, 903, 927, 1311
BstF5I	GGATG	7 cut(s)	475, 628, 991, 1017, 1182, 1495, 1717
BstFNI	CGCG	2 cut(s)	149, 1172
BstH2I	RGCGCY	2 cut(s)	443, 1288
BstHHI	GCGC	4 cut(s)	442, 499, 594, 1287
BstKTI	GATC	6 cut(s)	513, 947, 1186, 1645, 1666, 1806
BstMAI	GTCTC	2 cut(s)	888, 1307
BstMBI	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
BstMWI	GCNNNNNNNGC	5 cut(s)	216, 253, 307, 1082, 1400
BstNI	CCWGG	1 cut(s)	874
BstNSI	RCATGY	1 cut(s)	497
BstSCI	CCNGG	3 cut(s)	282, 417, 872
BstSFI	CTRYAG	2 cut(s)	1162, 1672
BstSLI	GKGCMC	2 cut(s)	413, 859
BstUI	CGCG	2 cut(s)	149, 1172
BstV1I	GCAGC	5 cut(s)	294, 1094, 1176, 1207, 1390
BstV2I	GAAGAC	2 cut(s)	286, 1836
BstX2I	RGATCY	2 cut(s)	944, 1642
BstYI	RGATCY	2 cut(s)	944, 1642
BsuI	GTATCC	1 cut(s)	1382
BsuRI	GGCC	6 cut(s)	417, 452, 483, 776, 1126, 1678
BtrI	CACGTC	1 cut(s)	190
BtsCI	GGATG	7 cut(s)	475, 628, 991, 1017, 1182, 1495, 1717
BtsI	GCAGTG	1 cut(s)	1068
BtsIMutI	CAGTG	2 cut(s)	1068, 1113
BveI	ACCTGC	3 cut(s)	166, 292, 1583
Cac8I	GCNNGC	8 cut(s)	151, 245, 485, 590, 1162, 1308, 1366, 1408
CaiI	CAGNNNCTG	2 cut(s)	307, 1787
CciI	TCATGA	1 cut(s)	1099
CfoI	GCGC	4 cut(s)	442, 499, 594, 1287
Cfr10I	RCCGGY	2 cut(s)	413, 483
Cfr13I	GGNCC	3 cut(s)	416, 481, 636
CseI	GACGC	1 cut(s)	155
Csp6I	GTAC	1 cut(s)	535
CspCI	CAANNNNNGTGG	2 cut(s)	436, 471
CviAII	CATG	8 cut(s)	167, 494, 1067, 1100, 1334, 1838, 1846, 1897
CviQI	GTAC	1 cut(s)	535
DdeI	CTNAG	7 cut(s)	93, 118, 206, 615, 903, 927, 1311
DpnI	GATC	6 cut(s)	512, 946, 1185, 1644, 1665, 1805
DpnII	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
EciI	GGCGGA	1 cut(s)	542
Eco130I	CCWWGG	1 cut(s)	777
Eco147I	AGGCCT	2 cut(s)	452, 776
Eco24I	GRGCYC	1 cut(s)	207
Eco47I	GGWCC	1 cut(s)	636
Eco88I	CYCGRG	2 cut(s)	836, 1301
EcoRI	GAATTC	1 cut(s)	757
EcoRII	CCWGG	1 cut(s)	872
EcoT14I	CCWWGG	1 cut(s)	777
EcoT22I	ATGCAT	2 cut(s)	994, 1474
EcoT38I	GRGCYC	1 cut(s)	207
ErhI	CCWWGG	1 cut(s)	777
FaeI	CATG	8 cut(s)	170, 497, 1070, 1103, 1337, 1841, 1849, 1900
FalI	AAGNNNNNCTT	2 cut(s)	351, 383
FaqI	GGGAC	3 cut(s)	248, 701, 1936
FatI	CATG	8 cut(s)	166, 493, 1066, 1099, 1333, 1837, 1845, 1896
FauI	CCCGC	2 cut(s)	581, 1165
FbaI	TGATCA	1 cut(s)	1183
FblI	GTMKAC	1 cut(s)	1263
Fnu4HI	GCNGC	7 cut(s)	308, 333, 1083, 1165, 1196, 1404, 1936
FokI	GGATG	7 cut(s)	482, 635, 998, 1024, 1189, 1502, 1724
FriOI	GRGCYC	1 cut(s)	207
Fsp4HI	GCNGC	7 cut(s)	308, 333, 1083, 1165, 1196, 1404, 1936
FspBI	CTAG	2 cut(s)	143, 1307
FspI	TGCGCA	1 cut(s)	498
GlaI	GCGC	4 cut(s)	441, 498, 593, 1286
GluI	GCNGC	7 cut(s)	308, 333, 1083, 1165, 1196, 1404, 1936
GsaI	CCCAGC	2 cut(s)	1284, 1558
HaeII	RGCGCY	2 cut(s)	443, 1288
HaeIII	GGCC	6 cut(s)	417, 452, 483, 776, 1126, 1678
HapII	CCGG	6 cut(s)	109, 284, 298, 414, 419, 484
HgaI	GACGC	1 cut(s)	155
HhaI	GCGC	4 cut(s)	442, 499, 594, 1287
Hin1II	CATG	8 cut(s)	170, 497, 1070, 1103, 1337, 1841, 1849, 1900
Hin6I	GCGC	4 cut(s)	440, 497, 592, 1285
HinP1I	GCGC	4 cut(s)	440, 497, 592, 1285
HindIII	AAGCTT	1 cut(s)	44
HinfI	GANTC	7 cut(s)	97, 261, 677, 908, 1603, 1784, 1902
HpaII	CCGG	6 cut(s)	109, 284, 298, 414, 419, 484
HphI	GGTGA	7 cut(s)	42, 73, 220, 241, 391, 1598, 1607
Hpy166II	GTNNAC	6 cut(s)	181, 429, 636, 729, 857, 1264
Hpy188I	TCNGA	8 cut(s)	94, 197, 260, 373, 552, 930, 1533, 1889
Hpy188III	TCNNGA	6 cut(s)	143, 467, 698, 893, 1100, 1301
Hpy8I	GTNNAC	6 cut(s)	181, 429, 636, 729, 857, 1264
HpyAV	CCTTC	7 cut(s)	63, 88, 353, 1476, 1624, 1852, 1866
HpyCH4III	ACNGT	2 cut(s)	505, 1835
HpyCH4IV	ACGT	4 cut(s)	189, 222, 431, 537
HpyF10VI	GCNNNNNNNGC	5 cut(s)	216, 253, 307, 1082, 1400
HpyF3I	CTNAG	7 cut(s)	93, 118, 206, 615, 903, 927, 1311
HpySE526I	ACGT	4 cut(s)	189, 222, 431, 537
Hsp92II	CATG	8 cut(s)	170, 497, 1070, 1103, 1337, 1841, 1849, 1900
HspAI	GCGC	4 cut(s)	440, 497, 592, 1285
KroI	GCCGGC	1 cut(s)	483
KroNI	GCCGGC	1 cut(s)	485
Ksp22I	TGATCA	1 cut(s)	1183
Kzo9I	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
LmnI	GCTCC	3 cut(s)	36, 978, 1349
Lsp1109I	GCAGC	5 cut(s)	294, 1094, 1176, 1207, 1390
LweI	GCATC	7 cut(s)	753, 979, 1095, 1443, 1481, 1713, 1865
MaeI	CTAG	2 cut(s)	143, 1307
MaeII	ACGT	4 cut(s)	189, 222, 431, 537
MaeIII	GTNAC	6 cut(s)	739, 826, 1135, 1228, 1604, 1864
MalI	GATC	6 cut(s)	512, 946, 1185, 1644, 1665, 1805
MboI	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
MboII	GAAGA	5 cut(s)	286, 1304, 1637, 1727, 1841
MfeI	CAATTG	2 cut(s)	354, 860
MflI	RGATCY	2 cut(s)	944, 1642
MhlI	GDGCHC	5 cut(s)	126, 207, 413, 859, 1354
MluI	ACGCGT	1 cut(s)	147
MlyI	GAGTC	3 cut(s)	270, 686, 1612
MmeI	TCCRAC	2 cut(s)	1485, 1617
Mph1103I	ATGCAT	2 cut(s)	994, 1474
MroNI	GCCGGC	1 cut(s)	483
MroXI	GAANNNNTTC	1 cut(s)	367
MseI	TTAA	2 cut(s)	1626, 1800
MslI	CAYNNNNRTG	4 cut(s)	395, 1071, 1256, 1338
MspI	CCGG	6 cut(s)	109, 284, 298, 414, 419, 484
MspR9I	CCNGG	3 cut(s)	284, 419, 874
MunI	CAATTG	2 cut(s)	354, 860
Mva1269I	GAATGC	1 cut(s)	801
MvaI	CCWGG	1 cut(s)	874
MvnI	CGCG	2 cut(s)	149, 1172
MwoI	GCNNNNNNNGC	5 cut(s)	216, 253, 307, 1082, 1400
NaeI	GCCGGC	1 cut(s)	485
NciI	CCSGG	2 cut(s)	284, 419
NdeII	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
NgoMIV	GCCGGC	1 cut(s)	483
NheI	GCTAGC	1 cut(s)	1306
NlaIII	CATG	8 cut(s)	170, 497, 1070, 1103, 1337, 1841, 1849, 1900
NlaIV	GGNNCC	2 cut(s)	412, 1792
NmeAIII	GCCGAG	1 cut(s)	416
NmuCI	GTSAC	2 cut(s)	1228, 1604
NsbI	TGCGCA	1 cut(s)	498
NsiI	ATGCAT	2 cut(s)	994, 1474
NspI	RCATGY	1 cut(s)	497
NspV	TTCGAA	1 cut(s)	1914
PaeR7I	CTCGAG	1 cut(s)	1301
PagI	TCATGA	1 cut(s)	1099
PaqCI	CACCTGC	2 cut(s)	292, 1583
PceI	AGGCCT	2 cut(s)	452, 776
PctI	GAATGC	1 cut(s)	801
PdiI	GCCGGC	1 cut(s)	485
PdmI	GAANNNNTTC	1 cut(s)	367
PfeI	GAWTC	4 cut(s)	97, 908, 1784, 1902
PflMI	CCANNNNNTGG	1 cut(s)	1241
PkrI	GCNGC	7 cut(s)	309, 334, 1084, 1166, 1197, 1405, 1937
PleI	GAGTC	3 cut(s)	269, 685, 1611
PpsI	GAGTC	3 cut(s)	269, 685, 1611
Ppu21I	YACGTR	1 cut(s)	538
Psp6I	CCWGG	1 cut(s)	872
PspFI	CCCAGC	2 cut(s)	1280, 1554
PspGI	CCWGG	1 cut(s)	872
PspN4I	GGNNCC	2 cut(s)	412, 1792
PspPI	GGNCC	3 cut(s)	416, 481, 636
PstI	CTGCAG	1 cut(s)	1166
PstNI	CAGNNNCTG	2 cut(s)	307, 1787
PsuI	RGATCY	2 cut(s)	944, 1642
RsaI	GTAC	1 cut(s)	536
RsaNI	GTAC	1 cut(s)	535
RseI	CAYNNNNRTG	4 cut(s)	395, 1071, 1256, 1338
SaqAI	TTAA	2 cut(s)	1626, 1800
SatI	GCNGC	7 cut(s)	308, 333, 1083, 1165, 1196, 1404, 1936
Sau3AI	GATC	6 cut(s)	510, 944, 1183, 1642, 1663, 1803
Sau96I	GGNCC	3 cut(s)	416, 481, 636
SchI	GAGTC	3 cut(s)	270, 686, 1612
ScrFI	CCNGG	3 cut(s)	284, 419, 874
SduI	GDGCHC	5 cut(s)	126, 207, 413, 859, 1354
SfaNI	GCATC	7 cut(s)	753, 979, 1095, 1443, 1481, 1713, 1865
SfcI	CTRYAG	2 cut(s)	1162, 1672
Sfr274I	CTCGAG	1 cut(s)	1301
SfuI	TTCGAA	1 cut(s)	1914
SinI	GGWCC	1 cut(s)	636
SlaI	CTCGAG	1 cut(s)	1301
SmiMI	CAYNNNNRTG	4 cut(s)	395, 1071, 1256, 1338
SmlI	CTYRAG	1 cut(s)	1301
SmoI	CTYRAG	1 cut(s)	1301
SseBI	AGGCCT	2 cut(s)	452, 776
SsiI	CCGC	7 cut(s)	241, 332, 377, 527, 588, 1172, 1935
SspI	AATATT	2 cut(s)	1000, 1438
SspMI	CTAG	2 cut(s)	143, 1307
StuI	AGGCCT	2 cut(s)	452, 776
StyD4I	CCNGG	3 cut(s)	282, 417, 872
StyI	CCWWGG	1 cut(s)	777
TaaI	ACNGT	2 cut(s)	505, 1835
TaiI	ACGT	4 cut(s)	192, 225, 434, 540
TaqI	TCGA	5 cut(s)	761, 892, 1276, 1302, 1914
TaqII	GACCGA	1 cut(s)	653
TauI	GCSGC	2 cut(s)	335, 1938
TfiI	GAWTC	4 cut(s)	97, 908, 1784, 1902
Tru1I	TTAA	2 cut(s)	1626, 1800
Tru9I	TTAA	2 cut(s)	1626, 1800
TscAI	CASTG	2 cut(s)	1068, 1120
TseFI	GTSAC	2 cut(s)	1228, 1604
TseI	GCWGC	5 cut(s)	307, 1082, 1164, 1195, 1403
Tsp45I	GTSAC	2 cut(s)	1228, 1604
TspDTI	ATGAA	5 cut(s)	621, 1028, 1088, 1728, 1834
TspGWI	ACGGA	1 cut(s)	1587
TspRI	CASTG	2 cut(s)	1068, 1120
Van91I	CCANNNNNTGG	1 cut(s)	1241
VneI	GTGCAC	1 cut(s)	855
VpaK11BI	GGWCC	1 cut(s)	636
XapI	RAATTY	4 cut(s)	20, 475, 757, 1687
XbaI	TCTAGA	1 cut(s)	142
XceI	RCATGY	1 cut(s)	497
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1916
XhoI	CTCGAG	1 cut(s)	1301
XmiI	GTMKAC	1 cut(s)	1263
XmnI	GAANNNNTTC	1 cut(s)	367
XspI	CTAG	2 cut(s)	143, 1307
Zsp2I	ATGCAT	2 cut(s)	994, 1474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.