Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0014443

Physical Location & Seq

Genomic Coordinates Forward (+)

16349103 .. 16351058

Gene Structure 1956 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g22270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGCAAAATCTTTCTCGCAAATTCTCGACAGTCCCAATTCACAAGCTTCTACCTTCGCAGCTTCACCAGCACAGACCACATCCGGCCGAAATCTTCTCGGAGTTTCAGCAGAGGCGGAAGCTCTTGGAGCACAAACTCGTCGCCGTTCTAGATGGATGCGCCGGCCTCCGCCAAGTCAAAGCGGTTCACGGTCACCTCCTCCTCCACGGCCTCGGCCAATGCTGCTACGTTCTCACGAAGCTCATCCGCACGCTCACGAAGCTCGACGTCCCCATGGACACGTATCCGCTCCTAGTCTTCCGACAGGTCAAGTACCCAAACCCGTTTCTCTGGACGGCTATGATCCGCGGGTACAGTATCCAAGGACCTGTTTCGGAATCGCTGAATTTTTATACTTGTATGAGGAAATCGGGTGTTGGACCGGTGTCGTTTACGTTCTCCGCGCTTTTTAAGGCCTGTGGGGATGTGCTTGATGTGAATTTGGGGCGGCAGATTCATGCCCACACGATTTTAGTTGGTGGGTTCGTGGTGGATTTGTATGTGGGGAACACTATGATCGATATGTATGTGAAATGTGGGTTTTTGGAGTGTGGGCGCAAGGTGTTTGATGAAATGCCTGAGAGGGATGTTGTTTCTTGGACTGAGTTGATTGTTGCTTATACCAAGGTTGGGGATATGGAATCAGCCCGGGAGTTGTTTGAGGGGTTGCCTGCAAAAGATATGGTGGCGTGGACTGCAATGGTTAACGGTTATGCTCAGAATGCCCATCCGAGAGAGGCTTTGGATTGCTTTGAGAGAATGCAGTGTGCTAGTGTAGGAACTGATGAGATTACGTTGGTTGGTCTCATTTCGGCTTGTGCACAATTAGGTGCATCTAAATATGCTAATTGGGTTCGGGACATTGCTGAGAAATCCGGTTTTGGGCCTACTGAAAATGTTCTTGTGGGTTCTGCATTGATAGATATGTACTCCAAGTGTGGAAGCTTGGATAATGCATTCAAGGTTTTTCAGGGAATGAAGGAAAGAAACGTATTTTCTTATAGCTCAATGATTTTGGGGTTTGCAATGCACGGTTGTGCAAGTGCAGCGATAGAATTATTTCATGAGATGTTGACGACGGAGATAAGACCTAACAAGGTTACTTTTATAGGGGTGCTGACAGCTTGCAGCCATGCTGGTATGGTTGATCAAGGTCGGCAGTTATTTGCAACCATGGAGAAGTACTATGATGTTGTTCCAACAGCTGATCATTACACTTGCATGGTAGACCTACTTGGTCGAGCTGGGCGCTTGGAAGAAGCTCTTGCGCTTGTTGAAACAATGCCAATAGATGCCCATGGAGGCGTGTGGGGAGCTCTACTCGGGGCATGTCGTACCCATGGGAATCCCGACATTGCTCAGATTACTGCTAACCATCTTTTTGAGCTTGAACCTGATAGTATTGGAAACTATGTAGTGCTCTCGAACATTTATGCATCAGCAGGAAGGTGGGATGAGGTTTCAAGAGTGAGGAAGTTGATGAGGGAAAAGGGTCTGAAAAAGAAACCTGCATATAGTTGGGTTGAAACAAAGAAAGGCGTAATTCACGAGTTCTGTGCGGGTGAAACAACCCATCCAGAGTTCATCGAGATTAAGAAGACGTTGGAGGACCTTCTGGACAGATTACTGGCTAATGGCTACCAGCCTAACCTGGATTCTGCGGCTTATGCTGTGGGAGATGATGAAAAGACGTGTATATTAATGTCTCATAGTGAAAAGCTAGCTTTAGCGTATGCATTACTCAGTACAGATTCTGTTTCCGCCATTAAGATTATGAAGAACATTAGGATTTGTGAAGACTGTCATTCGTTTATGTGCGGTGCATCCCAAGTTACGGGGAGGGAGATTGTCGTGAGAGATAACATGAGATTCCACCACTTCCACAATGGGACATGCTCTTGCGGAAACTTTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

652

Amino Acids

72.43

Weight (kDa)

6.58

Isoelectric Point (pI)

32.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	106 - 153	2.5e-06	PPR repeat family
PPR_2	PF13041	240 - 287	2e-07	PPR repeat family
PPR_2	PF13041	342 - 390	7e-08	PPR repeat family
E_motif	PF20431	460 - 522	1.9e-20	E motif
DYW_deaminase	PF14432	559 - 651	1.1e-40	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44230 AT5G44230
fragaria_vesca	FvH4_1g12540
malus_domestica	MD15G1252800.v1.1
prunus_persica	Prupe.7G161300_v2.0.a1
pyrus_communis	pycom15g22270
rosa_chinensis	RchiOBHm_Chr2g0100491
rosa_laevigata	RLG00000016976
rosa_multiflora	Rmu_sc0000528.1_g000023 Rmu_sc0020397.1_g000001
rosa_roxburghii	Rroxscaffold_2G00142190
rosa_rugosa	Rorug02G0088600
rosa_samantha	Rh2AG138300 Rh2BG141300 Rh2CG143500 Rh2DG143100
rosa_wichuraiana	Rw2G010810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	270
Acc36I	ACCTGC	1 cut(s)	1555
AccBSI	CCGCTC	1 cut(s)	289
AccI	GTMKAC	1 cut(s)	1266
AccII	CGCG	2 cut(s)	348, 443
AclWI	GGATC	1 cut(s)	337
AcoI	YGGCCR	2 cut(s)	84, 214
AcsI	RAATTY	3 cut(s)	20, 385, 478
AcyI	GRCGYC	1 cut(s)	267
AfaI	GTAC	6 cut(s)	314, 353, 968, 1223, 1375, 1786
AfiI	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
AflIII	ACRYGT	2 cut(s)	279, 1730
AgeI	ACCGGT	1 cut(s)	421
AgsI	TTSAA	5 cut(s)	1000, 1316, 1430, 1503, 1565
AjiI	CACGTC	1 cut(s)	1731
AjnI	CCWGG	1 cut(s)	1689
AleI	CACNNNNGTG	1 cut(s)	1074
Alw21I	GWGCWC	4 cut(s)	132, 862, 1357, 1461
Alw26I	GTCTC	2 cut(s)	848, 1749
Alw44I	GTGCAC	1 cut(s)	858
AlwI	GGATC	1 cut(s)	337
AlwNI	CAGNNNCTG	1 cut(s)	1793
Ama87I	CYCGRG	2 cut(s)	687, 1361
AoxI	GGCC	6 cut(s)	84, 163, 208, 214, 453, 923
ApaLI	GTGCAC	1 cut(s)	858
ApeKI	GCWGC	4 cut(s)	58, 222, 1085, 1167
ApoI	RAATTY	3 cut(s)	20, 385, 478
AseI	ATTAAT	1 cut(s)	1739
AsiGI	ACCGGT	1 cut(s)	421
Asp700I	GAANNNNTTC	2 cut(s)	936, 1098
AspLEI	GCGC	5 cut(s)	161, 445, 597, 1290, 1309
AspS9I	GGNCC	4 cut(s)	365, 419, 923, 1648
AsuC2I	CCSGG	2 cut(s)	688, 689
AsuHPI	GGTGA	3 cut(s)	56, 185, 1613
AsuNHI	GCTAGC	1 cut(s)	1759
AvaI	CYCGRG	2 cut(s)	687, 1361
AvaII	GGWCC	3 cut(s)	365, 419, 1648
BaeGI	GKGCMC	1 cut(s)	862
BanII	GRGCYC	1 cut(s)	1357
BauI	CACGAG	1 cut(s)	1586
BbsI	GAAGAC	3 cut(s)	289, 1643, 1842
Bbv12I	GWGCWC	4 cut(s)	132, 862, 1357, 1461
BbvI	GCAGC	4 cut(s)	70, 209, 1097, 1179
BccI	CCATC	4 cut(s)	146, 774, 1422, 1620
BceAI	ACGGC	3 cut(s)	128, 223, 351
BcgI	CGANNNNNNTGC	2 cut(s)	1577, 1611
BciT130I	CCWGG	1 cut(s)	1691
BciVI	GTATCC	2 cut(s)	294, 368
BclI	TGATCA	2 cut(s)	1186, 1246
BcnI	CCSGG	2 cut(s)	688, 689
BcoDI	GTCTC	2 cut(s)	848, 1749
BfaI	CTAG	4 cut(s)	149, 293, 810, 1760
BfoI	RGCGCY	1 cut(s)	1291
BfuAI	ACCTGC	1 cut(s)	1555
BfuI	GTATCC	2 cut(s)	294, 368
BisI	GCNGC	6 cut(s)	59, 223, 488, 1086, 1168, 1701
BlsI	GCNGC	6 cut(s)	60, 224, 489, 1087, 1169, 1702
BmcAI	AGTACT	1 cut(s)	1223
Bme1390I	CCNGG	3 cut(s)	688, 689, 1691
Bme18I	GGWCC	3 cut(s)	365, 419, 1648
BmeT110I	CYCGRG	2 cut(s)	687, 1361
BmgBI	CACGTC	1 cut(s)	1731
BmgT120I	GGNCC	4 cut(s)	365, 419, 923, 1648
BmrFI	CCNGG	3 cut(s)	688, 689, 1691
BmsI	GCATC	5 cut(s)	146, 881, 1321, 1484, 1871
BmtI	GCTAGC	1 cut(s)	1763
BoxI	GACNNNNGTC	1 cut(s)	424
BpiI	GAAGAC	3 cut(s)	289, 1643, 1842
BplI	GAGNNNNNCTC	2 cut(s)	1344, 1376
BpuMI	CCSGG	2 cut(s)	688, 689
Bsa29I	ATCGAT	1 cut(s)	558
BsaAI	YACGTR	1 cut(s)	282
BsaHI	GRCGYC	1 cut(s)	267
BsaI	GGTCTC	1 cut(s)	848
BsaWI	WCCGGW	2 cut(s)	421, 914
Bsc4I	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
Bse118I	RCCGGY	2 cut(s)	161, 421
Bse1I	ACTGG	1 cut(s)	1671
Bse3DI	GCAATG	4 cut(s)	744, 900, 1071, 1392
BseBI	CCWGG	1 cut(s)	1691
BseCI	ATCGAT	1 cut(s)	558
BseGI	GGATG	9 cut(s)	79, 161, 243, 469, 631, 766, 1498, 1612, 1862
BseLI	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
BseMI	GCAATG	4 cut(s)	744, 900, 1071, 1392
BseMII	CTCAG	6 cut(s)	609, 633, 770, 897, 1412, 1795
BseNI	ACTGG	1 cut(s)	1671
BseRI	GAGGAG	2 cut(s)	188, 191
BseSI	GKGCMC	1 cut(s)	862
BseX3I	CGGCCG	1 cut(s)	84
BseXI	GCAGC	4 cut(s)	70, 209, 1097, 1179
BseYI	CCCAGC	1 cut(s)	1283
BsgI	GTGCAG	1 cut(s)	1104
Bsh1236I	CGCG	2 cut(s)	348, 443
Bsh1285I	CGRYCG	1 cut(s)	87
BshFI	GGCC	6 cut(s)	86, 165, 210, 216, 455, 925
BshTI	ACCGGT	1 cut(s)	421
BshVI	ATCGAT	1 cut(s)	558
BsiEI	CGRYCG	1 cut(s)	87
BsiHKAI	GWGCWC	4 cut(s)	132, 862, 1357, 1461
BsiHKCI	CYCGRG	2 cut(s)	687, 1361
BsiSI	CCGG	5 cut(s)	83, 162, 422, 688, 915
BslFI	GGGAC	4 cut(s)	17, 254, 911, 1942
BslI	CCNNNNNNNGG	3 cut(s)	669, 921, 1873
BsmAI	GTCTC	2 cut(s)	848, 1749
BsmFI	GGGAC	4 cut(s)	17, 254, 911, 1942
BsmI	GAATGC	3 cut(s)	766, 804, 995
BsnI	GGCC	6 cut(s)	86, 165, 210, 216, 455, 925
Bso31I	GGTCTC	1 cut(s)	848
BsoBI	CYCGRG	2 cut(s)	687, 1361
Bsp1286I	GDGCHC	4 cut(s)	132, 862, 1357, 1461
Bsp143I	GATC	4 cut(s)	342, 555, 1186, 1246
Bsp19I	CCATGG	4 cut(s)	273, 1212, 1336, 1378
BspANI	GGCC	6 cut(s)	86, 165, 210, 216, 455, 925
BspCNI	CTCAG	6 cut(s)	610, 634, 769, 898, 1411, 1794
BspDI	ATCGAT	1 cut(s)	558
BspFNI	CGCG	2 cut(s)	348, 443
BspHI	TCATGA	1 cut(s)	1102
BspMI	ACCTGC	1 cut(s)	1555
BspOI	GCTAGC	1 cut(s)	1763
BspPI	GGATC	1 cut(s)	337
BspTNI	GGTCTC	1 cut(s)	848
BsrBI	CCGCTC	1 cut(s)	289
BsrDI	GCAATG	4 cut(s)	744, 900, 1071, 1392
BsrFI	RCCGGY	2 cut(s)	161, 421
BsrI	ACTGG	1 cut(s)	1671
BssAI	RCCGGY	2 cut(s)	161, 421
BssMI	GATC	4 cut(s)	342, 555, 1186, 1246
BssNI	GRCGYC	1 cut(s)	267
BssSI	CACGAG	1 cut(s)	1586
BssT1I	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
Bst2BI	CACGAG	1 cut(s)	1586
Bst2UI	CCWGG	1 cut(s)	1691
Bst4CI	ACNGT	6 cut(s)	31, 191, 356, 749, 1073, 1841
BstACI	GRCGYC	1 cut(s)	267
BstBAI	YACGTR	1 cut(s)	282
BstC8I	GCNNGC	5 cut(s)	163, 251, 711, 1165, 1761
BstDEI	CTNAG	6 cut(s)	618, 642, 756, 906, 1398, 1781
BstDSI	CCRYGG	6 cut(s)	205, 273, 346, 1212, 1336, 1378
BstEII	GGTNACC	1 cut(s)	191
BstF5I	GGATG	9 cut(s)	79, 161, 243, 469, 631, 766, 1498, 1612, 1862
BstFNI	CGCG	2 cut(s)	348, 443
BstH2I	RGCGCY	1 cut(s)	1291
BstHHI	GCGC	5 cut(s)	161, 445, 597, 1290, 1309
BstKTI	GATC	4 cut(s)	345, 558, 1189, 1249
BstMAI	GTCTC	2 cut(s)	848, 1749
BstMBI	GATC	4 cut(s)	342, 555, 1186, 1246
BstMCI	CGRYCG	1 cut(s)	87
BstMWI	GCNNNNNNNGC	8 cut(s)	67, 127, 222, 259, 734, 761, 1085, 1706
BstNI	CCWGG	1 cut(s)	1691
BstNSI	RCATGY	2 cut(s)	1371, 1935
BstPAI	GACNNNNGTC	1 cut(s)	424
BstPI	GGTNACC	1 cut(s)	191
BstSCI	CCNGG	3 cut(s)	686, 687, 1689
BstSLI	GKGCMC	1 cut(s)	862
BstUI	CGCG	2 cut(s)	348, 443
BstV1I	GCAGC	4 cut(s)	70, 209, 1097, 1179
BstV2I	GAAGAC	3 cut(s)	289, 1643, 1842
BstZI	CGGCCG	1 cut(s)	84
Bsu15I	ATCGAT	1 cut(s)	558
BsuI	GTATCC	2 cut(s)	294, 368
BsuRI	GGCC	6 cut(s)	86, 165, 210, 216, 455, 925
BsuTUI	ATCGAT	1 cut(s)	558
BtgI	CCRYGG	6 cut(s)	205, 273, 346, 1212, 1336, 1378
BtrI	CACGTC	1 cut(s)	1731
BtsCI	GGATG	9 cut(s)	79, 161, 243, 469, 631, 766, 1498, 1612, 1862
BtsI	GCAGTG	1 cut(s)	809
BtsIMutI	CAGTG	1 cut(s)	809
BveI	ACCTGC	1 cut(s)	1555
Cac8I	GCNNGC	5 cut(s)	163, 251, 711, 1165, 1761
CaiI	CAGNNNCTG	1 cut(s)	1793
CciI	TCATGA	1 cut(s)	1102
CfoI	GCGC	5 cut(s)	161, 445, 597, 1290, 1309
Cfr10I	RCCGGY	2 cut(s)	161, 421
Cfr13I	GGNCC	4 cut(s)	365, 419, 923, 1648
Cfr42I	CCGCGG	1 cut(s)	349
Cfr9I	CCCGGG	1 cut(s)	687
ClaI	ATCGAT	1 cut(s)	558
Csp6I	GTAC	6 cut(s)	313, 352, 967, 1222, 1374, 1785
CspAI	ACCGGT	1 cut(s)	421
CviQI	GTAC	6 cut(s)	313, 352, 967, 1222, 1374, 1785
DdeI	CTNAG	6 cut(s)	618, 642, 756, 906, 1398, 1781
DpnI	GATC	4 cut(s)	344, 557, 1188, 1248
DpnII	GATC	4 cut(s)	342, 555, 1186, 1246
EaeI	YGGCCR	2 cut(s)	84, 214
EagI	CGGCCG	1 cut(s)	84
EciI	GGCGGA	3 cut(s)	130, 158, 1789
Ecl136II	GAGCTC	1 cut(s)	1355
EclXI	CGGCCG	1 cut(s)	84
Eco130I	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
Eco147I	AGGCCT	1 cut(s)	455
Eco24I	GRGCYC	1 cut(s)	1357
Eco31I	GGTCTC	1 cut(s)	848
Eco47I	GGWCC	3 cut(s)	365, 419, 1648
Eco52I	CGGCCG	1 cut(s)	84
Eco53kI	GAGCTC	1 cut(s)	1355
Eco88I	CYCGRG	2 cut(s)	687, 1361
Eco91I	GGTNACC	1 cut(s)	191
EcoICRI	GAGCTC	1 cut(s)	1355
EcoO109I	RGGNCCY	2 cut(s)	365, 1648
EcoO65I	GGTNACC	1 cut(s)	191
EcoRII	CCWGG	1 cut(s)	1689
EcoT14I	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
EcoT22I	ATGCAT	3 cut(s)	997, 1477, 1777
EcoT38I	GRGCYC	1 cut(s)	1357
ErhI	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
FalI	AAGNNNNNCTT	2 cut(s)	1287, 1319
FaqI	GGGAC	4 cut(s)	17, 254, 911, 1942
FauI	CCCGC	2 cut(s)	341, 1591
FbaI	TGATCA	2 cut(s)	1186, 1246
FblI	GTMKAC	1 cut(s)	1266
Fnu4HI	GCNGC	6 cut(s)	59, 223, 488, 1086, 1168, 1701
FokI	GGATG	9 cut(s)	66, 168, 230, 476, 638, 753, 1505, 1599, 1849
FriOI	GRGCYC	1 cut(s)	1357
Fsp4HI	GCNGC	6 cut(s)	59, 223, 488, 1086, 1168, 1701
FspBI	CTAG	4 cut(s)	149, 293, 810, 1760
GlaI	GCGC	5 cut(s)	160, 444, 596, 1289, 1308
GluI	GCNGC	6 cut(s)	59, 223, 488, 1086, 1168, 1701
GsaI	CCCAGC	1 cut(s)	1287
HaeII	RGCGCY	1 cut(s)	1291
HaeIII	GGCC	6 cut(s)	86, 165, 210, 216, 455, 925
HapII	CCGG	5 cut(s)	83, 162, 422, 688, 915
HhaI	GCGC	5 cut(s)	161, 445, 597, 1290, 1309
Hin1I	GRCGYC	1 cut(s)	267
Hin6I	GCGC	5 cut(s)	159, 443, 595, 1288, 1307
HinP1I	GCGC	5 cut(s)	159, 443, 595, 1288, 1307
HincII	GTYRAC	2 cut(s)	745, 1113
HindII	GTYRAC	2 cut(s)	745, 1113
HindIII	AAGCTT	2 cut(s)	44, 982
HinfI	GANTC	7 cut(s)	377, 493, 680, 1384, 1694, 1790, 1908
HpaI	GTTAAC	1 cut(s)	745
HpaII	CCGG	5 cut(s)	83, 162, 422, 688, 915
HphI	GGTGA	3 cut(s)	56, 185, 1613
Hpy166II	GTNNAC	7 cut(s)	187, 432, 732, 745, 860, 1113, 1267
Hpy188I	TCNGA	7 cut(s)	100, 302, 376, 759, 771, 1401, 1536
Hpy8I	GTNNAC	7 cut(s)	187, 432, 732, 745, 860, 1113, 1267
Hpy99I	CGWCG	3 cut(s)	143, 269, 1120
HpyAV	CCTTC	4 cut(s)	63, 1012, 1479, 1661
HpyCH4III	ACNGT	6 cut(s)	31, 191, 356, 749, 1073, 1841
HpyCH4IV	ACGT	8 cut(s)	228, 267, 281, 434, 833, 1029, 1640, 1730
HpyF10VI	GCNNNNNNNGC	8 cut(s)	67, 127, 222, 259, 734, 761, 1085, 1706
HpyF3I	CTNAG	6 cut(s)	618, 642, 756, 906, 1398, 1781
HpySE526I	ACGT	8 cut(s)	228, 267, 281, 434, 833, 1029, 1640, 1730
Hsp92I	GRCGYC	1 cut(s)	267
HspAI	GCGC	5 cut(s)	159, 443, 595, 1288, 1307
KroI	GCCGGC	1 cut(s)	161
KroNI	GCCGGC	1 cut(s)	163
Ksp22I	TGATCA	2 cut(s)	1186, 1246
KspAI	GTTAAC	1 cut(s)	745
KspI	CCGCGG	1 cut(s)	349
Kzo9I	GATC	4 cut(s)	342, 555, 1186, 1246
LmnI	GCTCC	3 cut(s)	127, 294, 1352
Lsp1109I	GCAGC	4 cut(s)	70, 209, 1097, 1179
LweI	GCATC	5 cut(s)	146, 881, 1321, 1484, 1871
MaeI	CTAG	4 cut(s)	149, 293, 810, 1760
MaeII	ACGT	8 cut(s)	228, 267, 281, 434, 833, 1029, 1640, 1730
MaeIII	GTNAC	3 cut(s)	191, 1138, 1870
MalI	GATC	4 cut(s)	344, 557, 1188, 1248
MbiI	CCGCTC	1 cut(s)	289
MboI	GATC	4 cut(s)	342, 555, 1186, 1246
MboII	GAAGA	6 cut(s)	85, 289, 1307, 1648, 1828, 1847
MhlI	GDGCHC	4 cut(s)	132, 862, 1357, 1461
MluCI	AATT	8 cut(s)	20, 36, 385, 478, 863, 886, 1094, 1581
MmeI	TCCRAC	4 cut(s)	325, 397, 1262, 1623
Mph1103I	ATGCAT	3 cut(s)	997, 1477, 1777
MroNI	GCCGGC	1 cut(s)	161
MroXI	GAANNNNTTC	2 cut(s)	936, 1098
MseI	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
MslI	CAYNNNNRTG	2 cut(s)	1074, 1259
MspA1I	CMGCKG	2 cut(s)	348, 1244
MspI	CCGG	5 cut(s)	83, 162, 422, 688, 915
MspR9I	CCNGG	3 cut(s)	688, 689, 1691
Mva1269I	GAATGC	3 cut(s)	766, 804, 995
MvaI	CCWGG	1 cut(s)	1691
MvnI	CGCG	2 cut(s)	348, 443
MwoI	GCNNNNNNNGC	8 cut(s)	67, 127, 222, 259, 734, 761, 1085, 1706
NaeI	GCCGGC	1 cut(s)	163
NciI	CCSGG	2 cut(s)	688, 689
NcoI	CCATGG	4 cut(s)	273, 1212, 1336, 1378
NdeII	GATC	4 cut(s)	342, 555, 1186, 1246
NgoMIV	GCCGGC	1 cut(s)	161
NheI	GCTAGC	1 cut(s)	1759
NmeAIII	GCCGAG	1 cut(s)	192
NmuCI	GTSAC	1 cut(s)	191
NsiI	ATGCAT	3 cut(s)	997, 1477, 1777
NspI	RCATGY	2 cut(s)	1371, 1935
OliI	CACNNNNGTG	1 cut(s)	1074
PagI	TCATGA	1 cut(s)	1102
PceI	AGGCCT	1 cut(s)	455
PctI	GAATGC	3 cut(s)	766, 804, 995
PdiI	GCCGGC	1 cut(s)	163
PdmI	GAANNNNTTC	2 cut(s)	936, 1098
PfeI	GAWTC	7 cut(s)	377, 493, 680, 1384, 1694, 1790, 1908
PinAI	ACCGGT	1 cut(s)	421
PkrI	GCNGC	6 cut(s)	60, 224, 489, 1087, 1169, 1702
Ppu21I	YACGTR	1 cut(s)	282
PpuMI	RGGWCCY	2 cut(s)	365, 1648
PshAI	GACNNNNGTC	1 cut(s)	424
PshBI	ATTAAT	1 cut(s)	1739
Psp124BI	GAGCTC	1 cut(s)	1357
Psp5II	RGGWCCY	2 cut(s)	365, 1648
Psp6I	CCWGG	1 cut(s)	1689
PspEI	GGTNACC	1 cut(s)	191
PspFI	CCCAGC	1 cut(s)	1283
PspGI	CCWGG	1 cut(s)	1689
PspPI	GGNCC	4 cut(s)	365, 419, 923, 1648
PspPPI	RGGWCCY	2 cut(s)	365, 1648
PstNI	CAGNNNCTG	1 cut(s)	1793
PvuII	CAGCTG	1 cut(s)	1244
RsaI	GTAC	6 cut(s)	314, 353, 968, 1223, 1375, 1786
RsaNI	GTAC	6 cut(s)	313, 352, 967, 1222, 1374, 1785
RseI	CAYNNNNRTG	2 cut(s)	1074, 1259
SacI	GAGCTC	1 cut(s)	1357
SacII	CCGCGG	1 cut(s)	349
SaqAI	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
SatI	GCNGC	6 cut(s)	59, 223, 488, 1086, 1168, 1701
Sau3AI	GATC	4 cut(s)	342, 555, 1186, 1246
Sau96I	GGNCC	4 cut(s)	365, 419, 923, 1648
ScaI	AGTACT	1 cut(s)	1223
ScrFI	CCNGG	3 cut(s)	688, 689, 1691
SduI	GDGCHC	4 cut(s)	132, 862, 1357, 1461
SfaNI	GCATC	5 cut(s)	146, 881, 1321, 1484, 1871
Sfr303I	CCGCGG	1 cut(s)	349
SgrBI	CCGCGG	1 cut(s)	349
SinI	GGWCC	3 cut(s)	365, 419, 1648
SmaI	CCCGGG	1 cut(s)	689
SmiMI	CAYNNNNRTG	2 cut(s)	1074, 1259
Sse9I	AATT	8 cut(s)	20, 36, 385, 478, 863, 886, 1094, 1581
SseBI	AGGCCT	1 cut(s)	455
SspMI	CTAG	4 cut(s)	149, 293, 810, 1760
SstI	GAGCTC	1 cut(s)	1357
StuI	AGGCCT	1 cut(s)	455
StyD4I	CCNGG	3 cut(s)	686, 687, 1689
StyI	CCWWGG	6 cut(s)	273, 361, 663, 1212, 1336, 1378
TaaI	ACNGT	6 cut(s)	31, 191, 356, 749, 1073, 1841
TaiI	ACGT	8 cut(s)	231, 270, 284, 437, 836, 1032, 1643, 1733
TaqI	TCGA	6 cut(s)	26, 264, 558, 1279, 1463, 1626
TasI	AATT	8 cut(s)	20, 36, 385, 478, 863, 886, 1094, 1581
TatI	WGTACW	3 cut(s)	966, 1221, 1784
TauI	GCSGC	2 cut(s)	490, 1703
TfiI	GAWTC	7 cut(s)	377, 493, 680, 1384, 1694, 1790, 1908
Tru1I	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
Tru9I	TTAA	5 cut(s)	450, 744, 1632, 1739, 1806
TscAI	CASTG	1 cut(s)	809
TseFI	GTSAC	1 cut(s)	191
TseI	GCWGC	4 cut(s)	58, 222, 1085, 1167
Tsp45I	GTSAC	1 cut(s)	191
TspDTI	ATGAA	7 cut(s)	485, 624, 1031, 1091, 1612, 1737, 1829
TspGWI	ACGGA	1 cut(s)	1133
TspMI	CCCGGG	1 cut(s)	687
TspRI	CASTG	1 cut(s)	809
VneI	GTGCAC	1 cut(s)	858
VpaK11BI	GGWCC	3 cut(s)	365, 419, 1648
VspI	ATTAAT	1 cut(s)	1739
XapI	RAATTY	3 cut(s)	20, 385, 478
XbaI	TCTAGA	1 cut(s)	148
XceI	RCATGY	2 cut(s)	1371, 1935
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1922
XmaI	CCCGGG	1 cut(s)	687
XmiI	GTMKAC	1 cut(s)	1266
XmnI	GAANNNNTTC	2 cut(s)	936, 1098
XspI	CTAG	4 cut(s)	149, 293, 810, 1760
ZraI	GACGTC	1 cut(s)	268
ZrmI	AGTACT	1 cut(s)	1223
Zsp2I	ATGCAT	3 cut(s)	997, 1477, 1777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.