Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003021

Physical Location & Seq

Genomic Coordinates Reverse (-)

23660726 .. 23673807

Gene Structure 13082 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1271600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4518 bp

ATGGAAGTGCTCCCTTGTTCTACCATTCGTGTTGGACAATCTGATTGCCCTCAACAGAGTTCAGCAACCACTTCTGTCTGTGACGGGGAATCCAATTGCCTTGAACATGGAAAGGAAGTGCAAGCTGCAGATGACTTATTGCCAAATGTGGAAGGGCCTCGTTTAGGAAGACAAGGTGAAGTTCCAGAGGCAGTTGATGAATTGCAAACTTCTGAAGGATGTCAAAATGGAGCTTCAGTTCTTGATTGTTATCAATTGGAGGGTCAAAGGACATCCAGTGGTTCGCAAGATTTTGATGATGATGATATAAATGCACAGAATTACAGTGAACCCTGTGTGACCTCTGACAATAGTCATCTGATTGTAGATAGCAACGTAAATGCATTGCCAAACAACAGTAGGGAAGGAGAGTCATCTCTTTCAGAGTCCACATGGCTAGAAAGTGATGAATCTGTGGCACTGTGGGTCAAGTGGAGAGGGAAGTGGCAGACAGGAATACGATGTGCACGAGCAGACTGCCCATTATCGACTTTGAGAGCAAAACCAACTCATGATAGGAAAAAATATTTTGTGATATTTTTTCCACACACAAGGAATTATTCTTGGGCAGACACGCTACTTGTTCGCTCGATCAATGAATTTCCACACCCTATTGCATATAAAACGCACAAAGTTGGATTAAAAGTGGTTAAAGATTTAACCATAGCACGTCGGTTTATTATGCAAAAGTTAGCTGTTGGAATGCTGAATATTGTTGACCAATTTCATACTGAGGCTTTGATAGAGAATGCTCGTGATGTGGCCGTCTGGAAGGAGTTTGCTATGGAGGCTTCTCGTTGCAGTGGCTATTCTGATCTTGGAAAGATGCTTCTGAAGCTGCAAAGTATGATATCACAGACCTACCTAAATTCGGAATGGTTAGACCGTTCATATAACTTTTGGGTTCAGCAGTGTCAGAATGCGTGTAATGCTGCAACCATTGAAGTACTGAAGGAGGAACTAGTTGACTCTATTCTCTGGAATGAAGTCCAGTCTCTCCAGAATGCACCATTGCAGCCTACACTTGGTTCCGAGTGGAAAACCTGGAAGCATGAAGTTATGAAATGGTTTTCAACGTCTCATCCCATATCGAATAGTGTAGACATGCAGAAGCAGAGTAGTGATGGTCCCTTGACTCCAAATCCCCAAGTTGGCAGGAAAAGGCCAAAGCTTGAAGTTCGTCGTGCGGAGGCAAATGCTTCCCAGGTGGAAACTAGGGGATCAGATGAAGCTATAGCCATTGAAATTGACTCTGAATTCTTTAATAATCGAGACACTGCAAATGCTGCTACGTTAGCATCAGAGCCCTATAAAGAAGAAGATATGAAAGATTTACCTGCACCAACAGACACACCCGGGCGAGTTGCTGATAAATGGGACGGAGTATTACTTGAAGCTGGTAATTCTAAGCTCATCCAAACCAAAGGTGTGGAAATGACACCAGTCAATGAAGTAGCTGCCATAAGAACTTCTGAGCCTGGAAGTAAAAATCGACAGTGCATTGCTTATATAGAAGCCAAGGGAAGGCAATGTGTGAGATGGGCAAATGATGGTGATGTTTACTGTTGTGTGCATCTGTCCTCTCGCTTCATGGGAAGCTCTACAAAAGCTGAAGGGTCTCACTCTAGTGATCAACCACTGTGTGAAGGCACAACTGTCCTTGGAACTAAATGTAAGCATCGGTCTTTACAAGGCTCTTCATTTTGTAAGAAGCACAGACCAAAGAATGATATGAAAGCTATCTCAAATTTTCCAGAGAATACACTTAAGAGAAAGTTTGAGGAGAACATTTCTAATTTAGAGACCACAAAATGCAGAGAGATGGTATTGGTCAGAGATGTTGAAAGTCCTCTACAGGTGGATCCTGTCTCATATGTGCCTGGTAATGCCTTCTGTGAAAGAGGGAGCTTATTTGAGAAGTCCGAGTCCCCTGCCAAAGCTTGTAATATCACAGGAGAGCAGCGCTGCATTGGCTTTTGTTTGCGAGATGACAGCAACCCTTGTTTGGAAAGTCCAAAGAGGCATTCATTATATTGCGAAAAGCACCTCCCAAGCTGGCTCAAACGTGCAAGAAATGGTAAGAGTAGGATAATATCAAAAGAAGTCTTTGTAGATCTTTTGAGGGATTGTCACTCAGAGGAGCAAAAGTTTAATTTGCATCAAGCATGTGAGCTCTTTTACAAGCTCTTTAAAAGTATCTTATCTTTAAGAAATCCTGTTCCTAAGGATGTACAGTTTCAGTGGGCCCTATCTGAAGCTTCTAAAAATCTTGGTGTTGGGGAAATATTCTCAAAGTTGGTTTGCACTGAAAAGGAGAGACTCAGAAGGATTTGGGGCTTTAATGGTGATGAAGGTGAACATGTTTCATCGTCTGCAATGGAAGAACAGGCTCTGTTGTCATGGACTGTGGATGATAACCATGACAATGAACAAGTCATTAGGTGCAAAGTTTGTTCGCAGGAGTTTTCAGACAACCAAGAACTTGGAACTCACTGGATGGAAAATCATAAAAAGGAAGCACAATGGCTGTTTAGAGGTTATGCATGTGCCATCTGCCTGGATTCTTTTACTAATAAGAAAGTCTTGGAAAGTCATGTTCAGGAGAGACACTGTGTGCAATTTGTCGAACAATGCATGCTTTTCCAGTGTATTCCTTGTGGAAGCCACTTTGGTAATACAGATGAGTTATGGTTGCATGTGCTTGCAGTTCATCCTGATAATTTCAGGCTCTCAAAAACTCCTCGGCCTGTCCAGTCTATTGGTGACGATTCTCCAAGGAAGTTTGAGTTATACAATTCAGCTTCTGTGGAGAATAACACTGAGAATGTAAGTGGTCAACGAAAGTTTGTTTGCAGGTTTTGTGGGTTGAAGTTTGATTTGCTTCCCGATCTTGGTCGTCACCATCAAGCTGCTCACATGGGGCCAAGTTTAGCCAGTTCTCGGCCTTCAAAGAAGGGGATACGTTATTATGCTTATAAATTAAAATCTGGGAGACTTAGTCGTCCTAGATTGAGAAAAAGTTTGGCGGCAGCATCATATAGGATCAGGAATAGGGCAAATGTTACAATGAAGAAACGTATTCAATCATCGAAGTCACTTGGCACTGGAGGAATAAATGTACAGCGTCTTGCAACTGAGGCAGCAAGTCTTTCTAGATTAGGGGACTCACATTGTTCAGCAGTAGCAAGGATTCTTTTTTCTGAGATGCAGAAAACAAAATGTAGGCCAAGTAACCTAGATATTTTATCTGTTGCTCGCTCTGCTTGCTGCAGGGTCAGTCTCAAAGCCATGCTGGAGGGACAATATGGGGTCTTGCCAGAAAGTTTGTATCTTAGGGCAGCCAAGCTCTGCAGCGAGCATAATATTCAGATAGATTGGCATCAAGATGGATTTATTTGCCCTAAAGGATGCAAGGAATTCAGGGAATGCTTTGTGTCTCCGGTGATGCCTCTTCCAATTGGTGCTATGGAGCATAAATCTCCACCTTCATCAGATCCTTGTGATGATAAATGGAATGTGGATGAGAGCCACTATCTTATTGATGCACATCACTTGAGCCAAAGATCCTTCCAGAAGGCTCTTGTCTTGTGTGATGATATAAGTTTTGGACAGGAATTGGTTCCAGTGGTTTGTGTTGCAGATGAAGGCCAATTGGATTCTTATCCTGCCCTTGCAGGTGGTTCTAATGCTGGTATTTCCTTGCCATGGGAGAGCTTTACCTATATTATGAAGCCCTTGCTTCATCAATCCCCTGGACTCGATATAGAGAGTTTGCAATTGGGGTGTTCATGCACGCATTCTACATGCCGTCCTGAAACATGTGATCATGTTTACCTTTTTGATAATGACTATGATGATGCAAAAGACATCTACGGGAAATCCATGCGTTGCAGATTCCCATATGATGAGAGAGGTCGAATTATATTGGAGGAAGGGTACCTCGTTTACGAATGCAATCAGATGTGCAGCTGCACTAGATCTTGTCCTAATAGGGTTTTGCAGAATGGAGTAAGAGTGAAATTGGAAGTCTTCAAAACAGAGAAAAAGGGATGGGGAGTCAGGGCAGGTGAAGTGATCCTCCGCGGCACATTTGTTTGTGAGTACATAGGCGAGGTTTTAGATGAGCATGAAGCAAATGAGAGGCGCAACAGGTATGGAAAAGACAGTTACTTATATGAAATTGATGCTCATGTTAACGATATGAGTAGATTGGTCGAAGGACAGGCCCATCATGTAATTGACTCAACTAACTATGGAAATGTTTCAAGATTCATCAATCATAGCTGCTTGCCTAACCTTGTGAATCACCAAGTTCTTGTGGAAAGCATGGATAGTCTGTGTGCACATGTTGGTCTCTATGCAAATCGCGATATAGCTTTGGGGGAAGAAATAACGTATGACTATCGGTATAAACGTCTTCCTGGAGAAGGACATCCATGCCATTGTGGAGCTTCCGCGTGCCGGGGGCGACTCTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003824 GO:0005488 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016278 GO:0016279 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031399 GO:0032259 GO:0032268 GO:0033043 GO:0033044 GO:0034968 GO:0036211 GO:0040029 GO:0042054 GO:0043170 GO:0043412 GO:0043414 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045814 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051246 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:0140110 GO:1900109 GO:1901363 GO:1901564 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko00310 ko04211 map00310 map04211

Pfam Domains

Protein Families

Pre-SET SET zf-TRM13_CCCH

Protein Analysis

1506

Amino Acids

169.59

Weight (kDa)

6.34

Isoelectric Point (pI)

45.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C2H2_3rep	PF18868	862 - 986	1.8e-66	Zinc finger C2H2-type, 3 repeats
Pre-SET	PF05033	1247 - 1344	6.6e-13	Pre-SET motif
SET	PF00856	1363 - 1481	7.6e-23	SET domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0003021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23740 AT2G23740 AT2G23740 AT2G23740 AT2G23740 AT2G23740
fragaria_vesca	FvH4_1g14350 FvH4_1g14350 FvH4_1g14350 FvH4_1g14350 FvH4_1g14350 FvH4_1g14350 FvH4_1g14350
malus_domestica	MD02G1157000.v1.1 MD15G1271600.v1.1
prunus_persica	Prupe.7G146600_v2.0.a1 Prupe.7G146600_v2.0.a1 Prupe.7G146600_v2.0.a1 Prupe.7G146600_v2.0.a1 Prupe.7G146600_v2.0.a1 Prupe.7G146600_v2.0.a1 Prupe.7G146600_v2.0.a1 Prupe.7G146600_v2.0.a1
pyrus_communis	pycom02g12410 pycom15g23560
rosa_chinensis	RchiOBHm_Chr2g0103021
rosa_laevigata	RLG00000017201
rosa_multiflora	Rmu_sc0000388.1_g000017
rosa_roxburghii	Rroxscaffold_2G00139990
rosa_rugosa	Rorug02G0111400 Rorug02G0111400 Rorug02G0111500 Rorug02G0111500
rosa_samantha	Rh2AG159700 Rh2BG166200 Rh2CG166400 Rh2DG165100
rosa_wichuraiana	Rw2G012480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3015
AarI	CACCTGC	2 cut(s)	3704, 4094
AasI	GACNNNNNNGTC	1 cut(s)	3039
Acc36I	ACCTGC	4 cut(s)	1384, 2883, 3704, 4094
Acc65I	GGTACC	1 cut(s)	3975
AccB1I	GGYRCC	1 cut(s)	3975
AccI	GTMKAC	1 cut(s)	1140
AccII	CGCG	3 cut(s)	4122, 4407, 4496
AciI	CCGC	5 cut(s)	1226, 3065, 4120, 4122, 4494
AclWI	GGATC	7 cut(s)	1267, 1895, 1908, 3089, 3527, 3597, 4108
AcoI	YGGCCR	1 cut(s)	801
AcsI	RAATTY	5 cut(s)	638, 907, 1295, 1786, 3455
AcuI	CTGAAG	6 cut(s)	219, 234, 893, 1010, 1671, 2313
AdeI	CACNNNGTG	2 cut(s)	1682, 2653
AfaI	GTAC	5 cut(s)	987, 2271, 3159, 3977, 4142
AfeI	AGCGCT	1 cut(s)	2003
AflII	CTTAAG	1 cut(s)	1805
AflIII	ACRYGT	3 cut(s)	2398, 3857, 4384
AhlI	ACTAGT	1 cut(s)	1000
AjiI	CACGTC	1 cut(s)	710
AjnI	CCWGG	7 cut(s)	1082, 1242, 1516, 1918, 2595, 3790, 4459
AjuI	GAANNNNNNNTTGG	2 cut(s)	2047, 2079
AleI	CACNNNNGTG	1 cut(s)	1665
AloI	GAACNNNNNNTCC	2 cut(s)	222, 254
Alw21I	GWGCWC	4 cut(s)	12, 508, 2214, 4384
Alw44I	GTGCAC	2 cut(s)	504, 4380
AlwI	GGATC	7 cut(s)	1267, 1895, 1908, 3089, 3527, 3597, 4108
AlwNI	CAGNNNCTG	2 cut(s)	2431, 2843
Ama87I	CYCGRG	1 cut(s)	1394
Aor51HI	AGCGCT	1 cut(s)	2003
ApaI	GGGCCC	1 cut(s)	2287
ApaLI	GTGCAC	2 cut(s)	504, 4380
ApoI	RAATTY	5 cut(s)	638, 907, 1295, 1786, 3455
Asp700I	GAANNNNTTC	2 cut(s)	3657, 4300
Asp718I	GGTACC	1 cut(s)	3975
AspLEI	GCGC	2 cut(s)	2004, 4185
AspS9I	GGNCC	6 cut(s)	155, 1166, 2283, 2284, 2960, 4264
AsuC2I	CCSGG	3 cut(s)	1395, 1396, 4502
AsuHPI	GGTGA	9 cut(s)	188, 1604, 2396, 2405, 2813, 2930, 3493, 4118, 4337
AvaI	CYCGRG	1 cut(s)	1394
AvaII	GGWCC	1 cut(s)	1166
AxyI	CCTNAGG	1 cut(s)	2262
BaeGI	GKGCMC	3 cut(s)	508, 2287, 4384
BamHI	GGATCC	1 cut(s)	1900
BanI	GGYRCC	1 cut(s)	3975
BanII	GRGCYC	3 cut(s)	1347, 2214, 2287
BauI	CACGAG	2 cut(s)	507, 792
BbsI	GAAGAC	3 cut(s)	175, 4060, 4448
Bbv12I	GWGCWC	4 cut(s)	12, 508, 2214, 4384
BceAI	ACGGC	2 cut(s)	788, 3831
BcgI	CGANNNNNNTGC	4 cut(s)	498, 532, 1952, 1986
BciT130I	CCWGG	7 cut(s)	1084, 1244, 1518, 1920, 2597, 3792, 4461
BciVI	GTATCC	1 cut(s)	2991
BclI	TGATCA	2 cut(s)	1669, 3862
BcnI	CCSGG	3 cut(s)	1395, 1396, 4502
BcuI	ACTAGT	1 cut(s)	1000
BfaI	CTAG	8 cut(s)	437, 1001, 1254, 1665, 3045, 3192, 3275, 4014
BfmI	CTRYAG	5 cut(s)	126, 1272, 1892, 3307, 3388
BfoI	RGCGCY	1 cut(s)	2005
BfrI	CTTAAG	1 cut(s)	1805
BfuAI	ACCTGC	4 cut(s)	1384, 2883, 3704, 4094
BfuI	GTATCC	1 cut(s)	2991
BglII	AGATCT	2 cut(s)	2152, 4016
BmcAI	AGTACT	1 cut(s)	987
Bme18I	GGWCC	1 cut(s)	1166
BmeT110I	CYCGRG	1 cut(s)	1394
BmgBI	CACGTC	1 cut(s)	710
BmgT120I	GGNCC	6 cut(s)	155, 1166, 2283, 2284, 2960, 4264
BmiI	GGNNCC	7 cut(s)	1069, 1168, 1902, 2285, 2961, 3660, 3977
BoxI	GACNNNNGTC	2 cut(s)	351, 1481
BpiI	GAAGAC	3 cut(s)	175, 4060, 4448
BpmI	CTGGAG	4 cut(s)	1022, 3165, 3353, 4482
BpuEI	CTTGAG	1 cut(s)	3613
BpuMI	CCSGG	3 cut(s)	1395, 1396, 4502
BsaBI	GATNNNNATC	4 cut(s)	249, 3417, 3585, 3699
BsaI	GGTCTC	3 cut(s)	1662, 1835, 4397
BsaWI	WCCGGW	1 cut(s)	3478
Bse1I	ACTGG	9 cut(s)	276, 1030, 1481, 2537, 2683, 2791, 2973, 3148, 3662
Bse21I	CCTNAGG	1 cut(s)	2262
Bse3DI	GCAATG	5 cut(s)	383, 1049, 1539, 1574, 2421
Bse8I	GATNNNNATC	4 cut(s)	249, 3417, 3585, 3699
BseBI	CCWGG	7 cut(s)	1084, 1244, 1518, 1920, 2597, 3792, 4461
BseJI	GATNNNNATC	4 cut(s)	249, 3417, 3585, 3699
BseMI	GCAATG	5 cut(s)	383, 1049, 1539, 1574, 2421
BseMII	CTCAG	7 cut(s)	762, 1503, 2187, 2374, 2850, 3165, 3231
BseNI	ACTGG	9 cut(s)	276, 1030, 1481, 2537, 2683, 2791, 2973, 3148, 3662
BseRI	GAGGAG	3 cut(s)	1835, 2192, 2769
BseSI	GKGCMC	3 cut(s)	508, 2287, 4384
BsgI	GTGCAG	3 cut(s)	1362, 3994, 4024
Bsh1236I	CGCG	3 cut(s)	4122, 4407, 4496
BshNI	GGYRCC	1 cut(s)	3975
BsiHKAI	GWGCWC	4 cut(s)	12, 508, 2214, 4384
BsiHKCI	CYCGRG	1 cut(s)	1394
BsiSI	CCGG	3 cut(s)	1395, 3479, 4501
BslFI	GGGAC	5 cut(s)	1152, 1430, 1951, 3215, 3351
BsmBI	CGTCTC	1 cut(s)	1122
BsmFI	GGGAC	5 cut(s)	1152, 1430, 1951, 3215, 3351
BsmI	GAATGC	8 cut(s)	747, 793, 964, 1048, 2062, 3470, 3835, 3995
Bso31I	GGTCTC	3 cut(s)	1662, 1835, 4397
BsoBI	CYCGRG	1 cut(s)	1394
Bsp120I	GGGCCC	1 cut(s)	2283
Bsp1286I	GDGCHC	6 cut(s)	12, 508, 1347, 2214, 2287, 4384
Bsp1407I	TGTACA	2 cut(s)	2269, 3157
Bsp19I	CCATGG	1 cut(s)	3743
Bsp68I	TCGCGA	1 cut(s)	4407
BspACI	CCGC	5 cut(s)	1226, 3065, 4120, 4122, 4494
BspCNI	CTCAG	7 cut(s)	763, 1504, 2186, 2373, 2851, 3166, 3232
BspFNI	CGCG	3 cut(s)	4122, 4407, 4496
BspHI	TCATGA	1 cut(s)	550
BspLI	GGNNCC	7 cut(s)	1069, 1168, 1902, 2285, 2961, 3660, 3977
BspMAI	CTGCAG	3 cut(s)	130, 3311, 3392
BspMI	ACCTGC	4 cut(s)	1384, 2883, 3704, 4094
BspPI	GGATC	7 cut(s)	1267, 1895, 1908, 3089, 3527, 3597, 4108
BspQI	GCTCTTC	1 cut(s)	1741
BspT107I	GGYRCC	1 cut(s)	3975
BspTI	CTTAAG	1 cut(s)	1805
BspTNI	GGTCTC	3 cut(s)	1662, 1835, 4397
BsrDI	GCAATG	5 cut(s)	383, 1049, 1539, 1574, 2421
BsrGI	TGTACA	2 cut(s)	2269, 3157
BsrI	ACTGG	9 cut(s)	276, 1030, 1481, 2537, 2683, 2791, 2973, 3148, 3662
BssSI	CACGAG	2 cut(s)	507, 792
BssT1I	CCWWGG	4 cut(s)	1557, 1699, 2813, 3743
Bst2BI	CACGAG	2 cut(s)	507, 792
Bst2UI	CCWGG	7 cut(s)	1084, 1244, 1518, 1920, 2597, 3792, 4461
Bst6I	CTCTTC	2 cut(s)	1741, 3495
BstAFI	CTTAAG	1 cut(s)	1805
BstAPI	GCANNNNNTGC	1 cut(s)	1325
BstAUI	TGTACA	2 cut(s)	2269, 3157
BstDSI	CCRYGG	2 cut(s)	3743, 4120
BstENI	CCTNNNNNAGG	2 cut(s)	162, 4464
BstFNI	CGCG	3 cut(s)	4122, 4407, 4496
BstH2I	RGCGCY	1 cut(s)	2005
BstHHI	GCGC	2 cut(s)	2004, 4185
BstMWI	GCNNNNNNNGC	9 cut(s)	827, 874, 968, 1325, 1334, 2010, 3176, 3299, 3303
BstNI	CCWGG	7 cut(s)	1084, 1244, 1518, 1920, 2597, 3792, 4461
BstNSI	RCATGY	9 cut(s)	1147, 2208, 2402, 2586, 2677, 2738, 3846, 3861, 4388
BstPAI	GACNNNNGTC	2 cut(s)	351, 1481
BstSFI	CTRYAG	5 cut(s)	126, 1272, 1892, 3307, 3388
BstSLI	GKGCMC	3 cut(s)	508, 2287, 4384
BstUI	CGCG	3 cut(s)	4122, 4407, 4496
BstV2I	GAAGAC	3 cut(s)	175, 4060, 4448
BstX2I	RGATCY	5 cut(s)	1900, 2152, 3532, 3602, 4016
BstXI	CCANNNNNNTGG	4 cut(s)	1468, 2522, 2596, 2798
BstYI	RGATCY	5 cut(s)	1900, 2152, 3532, 3602, 4016
Bsu36I	CCTNAGG	1 cut(s)	2262
BsuI	GTATCC	1 cut(s)	2991
BtgI	CCRYGG	2 cut(s)	3743, 4120
BtrI	CACGTC	1 cut(s)	710
BtsI	GCAGTG	3 cut(s)	847, 956, 1314
BtuMI	TCGCGA	1 cut(s)	4407
BveI	ACCTGC	4 cut(s)	1384, 2883, 3704, 4094
CaiI	CAGNNNCTG	2 cut(s)	2431, 2843
CciI	TCATGA	1 cut(s)	550
CfoI	GCGC	2 cut(s)	2004, 4185
Cfr13I	GGNCC	6 cut(s)	155, 1166, 2283, 2284, 2960, 4264
Cfr42I	CCGCGG	1 cut(s)	4123
Cfr9I	CCCGGG	1 cut(s)	1394
CseI	GACGC	1 cut(s)	3152
Csp6I	GTAC	5 cut(s)	986, 2270, 3158, 3976, 4141
CviQI	GTAC	5 cut(s)	986, 2270, 3158, 3976, 4141
DraI	TTTAAA	1 cut(s)	2230
DraIII	CACNNNGTG	2 cut(s)	1682, 2653
DrdI	GACNNNNNNGTC	1 cut(s)	3039
DseDI	GACNNNNNNGTC	1 cut(s)	3039
EaeI	YGGCCR	1 cut(s)	801
Eam1104I	CTCTTC	2 cut(s)	1741, 3495
EarI	CTCTTC	2 cut(s)	1741, 3495
Ecl136II	GAGCTC	1 cut(s)	2212
Eco130I	CCWWGG	4 cut(s)	1557, 1699, 2813, 3743
Eco24I	GRGCYC	3 cut(s)	1347, 2214, 2287
Eco31I	GGTCTC	3 cut(s)	1662, 1835, 4397
Eco32I	GATATC	1 cut(s)	891
Eco47I	GGWCC	1 cut(s)	1166
Eco47III	AGCGCT	1 cut(s)	2003
Eco53kI	GAGCTC	1 cut(s)	2212
Eco57I	CTGAAG	6 cut(s)	219, 234, 893, 1010, 1671, 2313
Eco81I	CCTNAGG	1 cut(s)	2262
Eco88I	CYCGRG	1 cut(s)	1394
EcoICRI	GAGCTC	1 cut(s)	2212
EcoNI	CCTNNNNNAGG	2 cut(s)	162, 4464
EcoO109I	RGGNCCY	2 cut(s)	155, 2284
EcoRI	GAATTC	2 cut(s)	1295, 3455
EcoRII	CCWGG	7 cut(s)	1082, 1242, 1516, 1918, 2595, 3790, 4459
EcoRV	GATATC	1 cut(s)	891
EcoT14I	CCWWGG	4 cut(s)	1557, 1699, 2813, 3743
EcoT22I	ATGCAT	3 cut(s)	385, 2584, 2675
EcoT38I	GRGCYC	3 cut(s)	1347, 2214, 2287
ErhI	CCWWGG	4 cut(s)	1557, 1699, 2813, 3743
Esp3I	CGTCTC	1 cut(s)	1122
FalI	AAGNNNNNCTT	2 cut(s)	2606, 2638
FaqI	GGGAC	5 cut(s)	1152, 1430, 1951, 3215, 3351
FauNDI	CATATG	2 cut(s)	1912, 3940
FbaI	TGATCA	2 cut(s)	1669, 3862
FblI	GTMKAC	1 cut(s)	1140
FriOI	GRGCYC	3 cut(s)	1347, 2214, 2287
FspBI	CTAG	8 cut(s)	437, 1001, 1254, 1665, 3045, 3192, 3275, 4014
GlaI	GCGC	2 cut(s)	2003, 4184
GsuI	CTGGAG	4 cut(s)	1022, 3165, 3353, 4482
HaeII	RGCGCY	1 cut(s)	2005
HapII	CCGG	3 cut(s)	1395, 3479, 4501
HgaI	GACGC	1 cut(s)	3152
HhaI	GCGC	2 cut(s)	2004, 4185
Hin6I	GCGC	2 cut(s)	2002, 4183
HinP1I	GCGC	2 cut(s)	2002, 4183
HincII	GTYRAC	4 cut(s)	757, 1006, 2876, 4234
HindII	GTYRAC	4 cut(s)	757, 1006, 2876, 4234
HindIII	AAGCTT	3 cut(s)	1208, 1977, 2295
HpaI	GTTAAC	1 cut(s)	4234
HpaII	CCGG	3 cut(s)	1395, 3479, 4501
HphI	GGTGA	9 cut(s)	188, 1604, 2396, 2405, 2813, 2930, 3493, 4118, 4337
Hpy99I	CGWCG	2 cut(s)	714, 1224
HpyCH4IV	ACGT	9 cut(s)	375, 709, 1115, 1331, 2104, 3001, 3115, 4433, 4453
HpyF10VI	GCNNNNNNNGC	9 cut(s)	827, 874, 968, 1325, 1334, 2010, 3176, 3299, 3303
HpySE526I	ACGT	9 cut(s)	375, 709, 1115, 1331, 2104, 3001, 3115, 4433, 4453
HspAI	GCGC	2 cut(s)	2002, 4183
KpnI	GGTACC	1 cut(s)	3979
Ksp22I	TGATCA	2 cut(s)	1669, 3862
KspAI	GTTAAC	1 cut(s)	4234
KspI	CCGCGG	1 cut(s)	4123
LguI	GCTCTTC	1 cut(s)	1741
LmnI	GCTCC	6 cut(s)	15, 230, 1944, 2179, 3508, 4487
MaeI	CTAG	8 cut(s)	437, 1001, 1254, 1665, 3045, 3192, 3275, 4014
MaeII	ACGT	9 cut(s)	375, 709, 1115, 1331, 2104, 3001, 3115, 4433, 4453
MaeIII	GTNAC	9 cut(s)	80, 337, 2168, 2801, 2936, 3100, 3132, 3269, 4205
MfeI	CAATTG	5 cut(s)	94, 254, 3495, 3689, 3815
MflI	RGATCY	5 cut(s)	1900, 2152, 3532, 3602, 4016
MhlI	GDGCHC	6 cut(s)	12, 508, 1347, 2214, 2287, 4384
MmeI	TCCRAC	3 cut(s)	13, 655, 718
Mph1103I	ATGCAT	3 cut(s)	385, 2584, 2675
MroXI	GAANNNNTTC	2 cut(s)	3657, 4300
MslI	CAYNNNNRTG	3 cut(s)	1665, 2463, 3423
MspA1I	CMGCKG	2 cut(s)	4008, 4122
MspCI	CTTAAG	1 cut(s)	1805
MspI	CCGG	3 cut(s)	1395, 3479, 4501
MunI	CAATTG	5 cut(s)	94, 254, 3495, 3689, 3815
Mva1269I	GAATGC	8 cut(s)	747, 793, 964, 1048, 2062, 3470, 3835, 3995
MvaI	CCWGG	7 cut(s)	1084, 1244, 1518, 1920, 2597, 3792, 4461
MvnI	CGCG	3 cut(s)	4122, 4407, 4496
MwoI	GCNNNNNNNGC	9 cut(s)	827, 874, 968, 1325, 1334, 2010, 3176, 3299, 3303
NciI	CCSGG	3 cut(s)	1395, 1396, 4502
NcoI	CCATGG	1 cut(s)	3743
NdeI	CATATG	2 cut(s)	1912, 3940
NlaIV	GGNNCC	7 cut(s)	1069, 1168, 1902, 2285, 2961, 3660, 3977
NmeAIII	GCCGAG	2 cut(s)	2761, 2959
NmuCI	GTSAC	6 cut(s)	80, 337, 2168, 2801, 2936, 3132
NruI	TCGCGA	1 cut(s)	4407
NsiI	ATGCAT	3 cut(s)	385, 2584, 2675
NspI	RCATGY	9 cut(s)	1147, 2208, 2402, 2586, 2677, 2738, 3846, 3861, 4388
OliI	CACNNNNGTG	1 cut(s)	1665
PaeI	GCATGC	1 cut(s)	2677
PagI	TCATGA	1 cut(s)	550
PaqCI	CACCTGC	2 cut(s)	3704, 4094
PciI	ACATGT	3 cut(s)	2398, 3857, 4384
PciSI	GCTCTTC	1 cut(s)	1741
PcsI	WCGNNNNNNNCGW	2 cut(s)	505, 4450
PctI	GAATGC	8 cut(s)	747, 793, 964, 1048, 2062, 3470, 3835, 3995
PdmI	GAANNNNTTC	2 cut(s)	3657, 4300
PfeI	GAWTC	9 cut(s)	89, 449, 2600, 2807, 3229, 3695, 3933, 4308, 4342
PflFI	GACNNNGTC	1 cut(s)	3036
PfoI	TCCNGGA	1 cut(s)	4459
PscI	ACATGT	3 cut(s)	2398, 3857, 4384
PshAI	GACNNNNGTC	2 cut(s)	351, 1481
PsiI	TTATAA	1 cut(s)	3015
Psp124BI	GAGCTC	1 cut(s)	2214
Psp6I	CCWGG	7 cut(s)	1082, 1242, 1516, 1918, 2595, 3790, 4459
PspGI	CCWGG	7 cut(s)	1082, 1242, 1516, 1918, 2595, 3790, 4459
PspN4I	GGNNCC	7 cut(s)	1069, 1168, 1902, 2285, 2961, 3660, 3977
PspOMI	GGGCCC	1 cut(s)	2283
PspPI	GGNCC	6 cut(s)	155, 1166, 2283, 2284, 2960, 4264
PstI	CTGCAG	3 cut(s)	130, 3311, 3392
PstNI	CAGNNNCTG	2 cut(s)	2431, 2843
PsuI	RGATCY	5 cut(s)	1900, 2152, 3532, 3602, 4016
PsyI	GACNNNGTC	1 cut(s)	3036
PvuII	CAGCTG	1 cut(s)	4008
RruI	TCGCGA	1 cut(s)	4407
RsaI	GTAC	5 cut(s)	987, 2271, 3159, 3977, 4142
RsaNI	GTAC	5 cut(s)	986, 2270, 3158, 3976, 4141
RseI	CAYNNNNRTG	3 cut(s)	1665, 2463, 3423
SacI	GAGCTC	1 cut(s)	2214
SacII	CCGCGG	1 cut(s)	4123
SapI	GCTCTTC	1 cut(s)	1741
Sau96I	GGNCC	6 cut(s)	155, 1166, 2283, 2284, 2960, 4264
ScaI	AGTACT	1 cut(s)	987
SduI	GDGCHC	6 cut(s)	12, 508, 1347, 2214, 2287, 4384
SfcI	CTRYAG	5 cut(s)	126, 1272, 1892, 3307, 3388
Sfr303I	CCGCGG	1 cut(s)	4123
SgrBI	CCGCGG	1 cut(s)	4123
SinI	GGWCC	1 cut(s)	1166
SmaI	CCCGGG	1 cut(s)	1396
SmiMI	CAYNNNNRTG	3 cut(s)	1665, 2463, 3423
SmlI	CTYRAG	2 cut(s)	1805, 3592
SmoI	CTYRAG	2 cut(s)	1805, 3592
SpeI	ACTAGT	1 cut(s)	1000
SphI	GCATGC	1 cut(s)	2677
SsiI	CCGC	5 cut(s)	1226, 3065, 4120, 4122, 4494
SspI	AATATT	4 cut(s)	566, 751, 2325, 3403
SspMI	CTAG	8 cut(s)	437, 1001, 1254, 1665, 3045, 3192, 3275, 4014
SstI	GAGCTC	1 cut(s)	2214
StyI	CCWWGG	4 cut(s)	1557, 1699, 2813, 3743
TaiI	ACGT	9 cut(s)	378, 712, 1118, 1334, 2107, 3004, 3118, 4436, 4456
TaqII	GACCGA	1 cut(s)	1710
TatI	WGTACW	4 cut(s)	985, 2269, 3157, 4140
TauI	GCSGC	2 cut(s)	3068, 4125
TfiI	GAWTC	9 cut(s)	89, 449, 2600, 2807, 3229, 3695, 3933, 4308, 4342
TseFI	GTSAC	6 cut(s)	80, 337, 2168, 2801, 2936, 3132
Tsp45I	GTSAC	6 cut(s)	80, 337, 2168, 2801, 2936, 3132
TspGWI	ACGGA	1 cut(s)	1434
TspMI	CCCGGG	1 cut(s)	1394
Tth111I	GACNNNGTC	1 cut(s)	3036
Vha464I	CTTAAG	1 cut(s)	1805
VneI	GTGCAC	2 cut(s)	504, 4380
VpaK11BI	GGWCC	1 cut(s)	1166
XagI	CCTNNNNNAGG	2 cut(s)	162, 4464
XapI	RAATTY	5 cut(s)	638, 907, 1295, 1786, 3455
XbaI	TCTAGA	1 cut(s)	3191
XceI	RCATGY	9 cut(s)	1147, 2208, 2402, 2586, 2677, 2738, 3846, 3861, 4388
XmaI	CCCGGG	1 cut(s)	1394
XmiI	GTMKAC	1 cut(s)	1140
XmnI	GAANNNNTTC	2 cut(s)	3657, 4300
XspI	CTAG	8 cut(s)	437, 1001, 1254, 1665, 3045, 3192, 3275, 4014
ZrmI	AGTACT	1 cut(s)	987
Zsp2I	ATGCAT	3 cut(s)	385, 2584, 2675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.