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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G50370 AT3G50370 |
| fragaria_vesca | FvH4_5g28170 FvH4_5g28170 |
| malus_domestica | MD02G1171200.v1.1 MD15G1283100.v1.1 |
| prunus_persica | Prupe.7G134200_v2.0.a1 Prupe.7G134200_v2.0.a1 Prupe.7G134200_v2.0.a1 Prupe.7G134200_v2.0.a1 Prupe.7G134200_v2.0.a1 Prupe.7G134200_v2.0.a1 |
| pyrus_communis | pycom02g13790 pycom15g24620 |
| rosa_chinensis | RchiOBHm_Chr2g0105371 |
| rosa_laevigata | RLG00000017420 |
| rosa_multiflora | Rmu_sc0001407.1_g000023 |
| rosa_roxburghii | Rroxscaffold_2G00138260 |
| rosa_rugosa | Rorug02G0127800 Rorug02G0127900 Rorug02G0127900 Rorug02G0127900 |
| rosa_samantha | Rh2AG178600 Rh2BG187000 Rh2CG183100 Rh2DG184500 |
| rosa_wichuraiana | Rw2G014020 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1463 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 3383 |
| Acc16I | TGCGCA | 1 cut(s) | 236 |
| Acc36I | ACCTGC | 3 cut(s) | 290, 1463, 2831 |
| Acc65I | GGTACC | 3 cut(s) | 3472, 7229, 7395 |
| AccB1I | GGYRCC | 4 cut(s) | 2514, 3472, 7229, 7395 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 1169, 4496, 6155, 6790 |
| AccBSI | CCGCTC | 1 cut(s) | 5760 |
| AccI | GTMKAC | 3 cut(s) | 1330, 3989, 7116 |
| AccII | CGCG | 1 cut(s) | 7027 |
| AcoI | YGGCCR | 4 cut(s) | 2353, 6260, 6831, 7315 |
| AcsI | RAATTY | 9 cut(s) | 2567, 2668, 3522, 3625, 4234, 4534, 6050, 6283, 6971 |
| AflIII | ACRYGT | 1 cut(s) | 3682 |
| AgeI | ACCGGT | 1 cut(s) | 1395 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 741, 4890 |
| AhlI | ACTAGT | 2 cut(s) | 850, 6434 |
| AjiI | CACGTC | 2 cut(s) | 5249, 5730 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3019, 3051 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 5375, 5407 |
| Alw21I | GWGCWC | 4 cut(s) | 2398, 3532, 6442, 7175 |
| Alw44I | GTGCAC | 1 cut(s) | 6438 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 4562, 4673, 5918, 6032, 7417 |
| Ama87I | CYCGRG | 4 cut(s) | 3060, 4398, 5109, 7019 |
| ApaI | GGGCCC | 1 cut(s) | 4483 |
| ApaLI | GTGCAC | 1 cut(s) | 6438 |
| ApoI | RAATTY | 9 cut(s) | 2567, 2668, 3522, 3625, 4234, 4534, 6050, 6283, 6971 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2333, 2365 |
| AseI | ATTAAT | 1 cut(s) | 2916 |
| AsiGI | ACCGGT | 1 cut(s) | 1395 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1060, 2411, 4376 |
| Asp718I | GGTACC | 3 cut(s) | 3472, 7229, 7395 |
| AspA2I | CCTAGG | 2 cut(s) | 3388, 4751 |
| AspLEI | GCGC | 6 cut(s) | 237, 4435, 5433, 6454, 6601, 6756 |
| AsuC2I | CCSGG | 3 cut(s) | 12, 1607, 6484 |
| AsuNHI | GCTAGC | 1 cut(s) | 1783 |
| AvaI | CYCGRG | 4 cut(s) | 3060, 4398, 5109, 7019 |
| AvrII | CCTAGG | 2 cut(s) | 3388, 4751 |
| AxyI | CCTNAGG | 2 cut(s) | 3477, 3921 |
| BaeGI | GKGCMC | 2 cut(s) | 4483, 6442 |
| BaeI | ACNNNNGTAYC | 6 cut(s) | 1414, 1447, 2645, 2678, 3453, 3486 |
| BalI | TGGCCA | 4 cut(s) | 2355, 6262, 6833, 7317 |
| BamHI | GGATCC | 3 cut(s) | 1564, 1609, 3362 |
| BanI | GGYRCC | 4 cut(s) | 2514, 3472, 7229, 7395 |
| BanII | GRGCYC | 6 cut(s) | 269, 2398, 4088, 4483, 4814, 7175 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 6949, 6981, 7448, 7480 |
| BauI | CACGAG | 2 cut(s) | 2727, 7504 |
| BbsI | GAAGAC | 4 cut(s) | 2430, 4350, 5843, 7269 |
| Bbv12I | GWGCWC | 4 cut(s) | 2398, 3532, 6442, 7175 |
| BbvCI | CCTCAGC | 2 cut(s) | 399, 2803 |
| BceAI | ACGGC | 1 cut(s) | 5892 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 722, 756, 1203, 1237 |
| BciVI | GTATCC | 4 cut(s) | 2673, 3266, 4306, 6223 |
| BclI | TGATCA | 4 cut(s) | 385, 6043, 6166, 7216 |
| BcnI | CCSGG | 3 cut(s) | 12, 1607, 6484 |
| BcuI | ACTAGT | 2 cut(s) | 850, 6434 |
| BfmI | CTRYAG | 9 cut(s) | 297, 1350, 1470, 2773, 3783, 5400, 5829, 6603, 7065 |
| BfoI | RGCGCY | 2 cut(s) | 4436, 6455 |
| BfuAI | ACCTGC | 3 cut(s) | 290, 1463, 2831 |
| BfuI | GTATCC | 4 cut(s) | 2673, 3266, 4306, 6223 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 4092 |
| BglII | AGATCT | 2 cut(s) | 4322, 6226 |
| BlnI | CCTAGG | 2 cut(s) | 3388, 4751 |
| BlpI | GCTNAGC | 2 cut(s) | 1992, 4563 |
| BmcAI | AGTACT | 4 cut(s) | 694, 3700, 4014, 5158 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 741, 4890 |
| BmeT110I | CYCGRG | 4 cut(s) | 3060, 4398, 5109, 7019 |
| BmgBI | CACGTC | 2 cut(s) | 5249, 5730 |
| BmrI | ACTGGG | 3 cut(s) | 2152, 7115, 7149 |
| BmtI | GCTAGC | 1 cut(s) | 1787 |
| BmuI | ACTGGG | 3 cut(s) | 2152, 7115, 7149 |
| BoxI | GACNNNNGTC | 1 cut(s) | 7050 |
| BpiI | GAAGAC | 4 cut(s) | 2430, 4350, 5843, 7269 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 2710, 2742, 3115, 3147, 4588, 4620 |
| BpmI | CTGGAG | 8 cut(s) | 2104, 2311, 4200, 4269, 4446, 4932, 6054, 6564 |
| Bpu10I | CCTNAGC | 4 cut(s) | 268, 399, 2803, 3983 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1992, 4563 |
| BpuEI | CTTGAG | 9 cut(s) | 986, 1272, 1733, 2308, 4194, 4392, 4828, 6649, 7468 |
| BpuMI | CCSGG | 3 cut(s) | 12, 1607, 6484 |
| BsaAI | YACGTR | 1 cut(s) | 7468 |
| BsaBI | GATNNNNATC | 4 cut(s) | 384, 2880, 7215, 7221 |
| BsaI | GGTCTC | 4 cut(s) | 2238, 4594, 6110, 6233 |
| BsaWI | WCCGGW | 4 cut(s) | 1395, 3845, 3868, 4064 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 135, 165, 2546, 2576, 2753, 2783, 5375, 5405 |
| Bse118I | RCCGGY | 4 cut(s) | 535, 1395, 1622, 6038 |
| Bse21I | CCTNAGG | 2 cut(s) | 3477, 3921 |
| Bse3DI | GCAATG | 5 cut(s) | 230, 2533, 3624, 6479, 6998 |
| Bse8I | GATNNNNATC | 4 cut(s) | 384, 2880, 7215, 7221 |
| BseJI | GATNNNNATC | 4 cut(s) | 384, 2880, 7215, 7221 |
| BseMI | GCAATG | 5 cut(s) | 230, 2533, 3624, 6479, 6998 |
| BseRI | GAGGAG | 8 cut(s) | 408, 1817, 1958, 2047, 3293, 3368, 4612, 6410 |
| BseSI | GKGCMC | 2 cut(s) | 4483, 6442 |
| BseYI | CCCAGC | 2 cut(s) | 3874, 7438 |
| BsgI | GTGCAG | 1 cut(s) | 6732 |
| Bsh1236I | CGCG | 1 cut(s) | 7027 |
| BshNI | GGYRCC | 4 cut(s) | 2514, 3472, 7229, 7395 |
| BshTI | ACCGGT | 1 cut(s) | 1395 |
| BsiHKAI | GWGCWC | 4 cut(s) | 2398, 3532, 6442, 7175 |
| BsiHKCI | CYCGRG | 4 cut(s) | 3060, 4398, 5109, 7019 |
| BsmBI | CGTCTC | 2 cut(s) | 3939, 5736 |
| BsmI | GAATGC | 3 cut(s) | 1815, 3178, 3423 |
| Bso31I | GGTCTC | 4 cut(s) | 2238, 4594, 6110, 6233 |
| BsoBI | CYCGRG | 4 cut(s) | 3060, 4398, 5109, 7019 |
| Bsp120I | GGGCCC | 1 cut(s) | 4479 |
| Bsp1286I | GDGCHC | 8 cut(s) | 269, 2398, 3532, 4088, 4483, 4814, 6442, 7175 |
| Bsp1407I | TGTACA | 1 cut(s) | 751 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1992, 4563 |
| Bsp19I | CCATGG | 5 cut(s) | 2626, 4483, 6702, 6816, 6979 |
| BspFNI | CGCG | 1 cut(s) | 7027 |
| BspHI | TCATGA | 2 cut(s) | 3040, 3532 |
| BspMAI | CTGCAG | 3 cut(s) | 301, 1474, 5833 |
| BspMI | ACCTGC | 3 cut(s) | 290, 1463, 2831 |
| BspOI | GCTAGC | 1 cut(s) | 1787 |
| BspQI | GCTCTTC | 2 cut(s) | 1906, 1914 |
| BspT107I | GGYRCC | 4 cut(s) | 2514, 3472, 7229, 7395 |
| BspTNI | GGTCTC | 4 cut(s) | 2238, 4594, 6110, 6233 |
| BsrBI | CCGCTC | 1 cut(s) | 5760 |
| BsrDI | GCAATG | 5 cut(s) | 230, 2533, 3624, 6479, 6998 |
| BsrFI | RCCGGY | 4 cut(s) | 535, 1395, 1622, 6038 |
| BsrGI | TGTACA | 1 cut(s) | 751 |
| BssAI | RCCGGY | 4 cut(s) | 535, 1395, 1622, 6038 |
| BssNAI | GTATAC | 1 cut(s) | 3990 |
| BssSI | CACGAG | 2 cut(s) | 2727, 7504 |
| Bst1107I | GTATAC | 1 cut(s) | 3990 |
| Bst2BI | CACGAG | 2 cut(s) | 2727, 7504 |
| BstAUI | TGTACA | 1 cut(s) | 751 |
| BstBAI | YACGTR | 1 cut(s) | 7468 |
| BstDSI | CCRYGG | 5 cut(s) | 2626, 4483, 6702, 6816, 6979 |
| BstEII | GGTNACC | 1 cut(s) | 6801 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2698, 2773 |
| BstFNI | CGCG | 1 cut(s) | 7027 |
| BstH2I | RGCGCY | 2 cut(s) | 4436, 6455 |
| BstHHI | GCGC | 6 cut(s) | 237, 4435, 5433, 6454, 6601, 6756 |
| BstNSI | RCATGY | 9 cut(s) | 721, 1988, 2624, 3346, 3686, 3949, 5629, 5809, 6772 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 7050 |
| BstPI | GGTNACC | 1 cut(s) | 6801 |
| BstSFI | CTRYAG | 9 cut(s) | 297, 1350, 1470, 2773, 3783, 5400, 5829, 6603, 7065 |
| BstSLI | GKGCMC | 2 cut(s) | 4483, 6442 |
| BstUI | CGCG | 1 cut(s) | 7027 |
| BstV2I | GAAGAC | 4 cut(s) | 2430, 4350, 5843, 7269 |
| BstX2I | RGATCY | 6 cut(s) | 1564, 1609, 3362, 4322, 5360, 6226 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2351, 7313 |
| BstYI | RGATCY | 6 cut(s) | 1564, 1609, 3362, 4322, 5360, 6226 |
| BstZ17I | GTATAC | 1 cut(s) | 3990 |
| Bsu36I | CCTNAGG | 2 cut(s) | 3477, 3921 |
| BsuI | GTATCC | 4 cut(s) | 2673, 3266, 4306, 6223 |
| BtgI | CCRYGG | 5 cut(s) | 2626, 4483, 6702, 6816, 6979 |
| BtgZI | GCGATG | 1 cut(s) | 62 |
| BtrI | CACGTC | 2 cut(s) | 5249, 5730 |
| BveI | ACCTGC | 3 cut(s) | 290, 1463, 2831 |
| CaiI | CAGNNNCTG | 5 cut(s) | 4562, 4673, 5918, 6032, 7417 |
| CciI | TCATGA | 2 cut(s) | 3040, 3532 |
| CfoI | GCGC | 6 cut(s) | 237, 4435, 5433, 6454, 6601, 6756 |
| Cfr10I | RCCGGY | 4 cut(s) | 535, 1395, 1622, 6038 |
| CseI | GACGC | 2 cut(s) | 2780, 4617 |
| CsiI | ACCWGGT | 1 cut(s) | 6553 |
| CspAI | ACCGGT | 1 cut(s) | 1395 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 198, 233, 6866, 6901 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 3383 |
| DriI | GACNNNNNGTC | 2 cut(s) | 741, 4890 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 3383 |
| EaeI | YGGCCR | 4 cut(s) | 2353, 6260, 6831, 7315 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 741, 4890 |
| EciI | GGCGGA | 1 cut(s) | 896 |
| Ecl136II | GAGCTC | 2 cut(s) | 2396, 7173 |
| Eco147I | AGGCCT | 3 cut(s) | 166, 316, 610 |
| Eco24I | GRGCYC | 6 cut(s) | 269, 2398, 4088, 4483, 4814, 7175 |
| Eco31I | GGTCTC | 4 cut(s) | 2238, 4594, 6110, 6233 |
| Eco32I | GATATC | 1 cut(s) | 2488 |
| Eco53kI | GAGCTC | 2 cut(s) | 2396, 7173 |
| Eco81I | CCTNAGG | 2 cut(s) | 3477, 3921 |
| Eco88I | CYCGRG | 4 cut(s) | 3060, 4398, 5109, 7019 |
| Eco91I | GGTNACC | 1 cut(s) | 6801 |
| EcoICRI | GAGCTC | 2 cut(s) | 2396, 7173 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2698, 2773 |
| EcoO65I | GGTNACC | 1 cut(s) | 6801 |
| EcoRI | GAATTC | 4 cut(s) | 2567, 3522, 4534, 6283 |
| EcoRV | GATATC | 1 cut(s) | 2488 |
| EcoT22I | ATGCAT | 2 cut(s) | 3348, 5494 |
| EcoT38I | GRGCYC | 6 cut(s) | 269, 2398, 4088, 4483, 4814, 7175 |
| Esp3I | CGTCTC | 2 cut(s) | 3939, 5736 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1881, 1913, 6127, 6159 |
| FauI | CCCGC | 1 cut(s) | 6505 |
| FauNDI | CATATG | 2 cut(s) | 55, 3657 |
| FbaI | TGATCA | 4 cut(s) | 385, 6043, 6166, 7216 |
| FblI | GTMKAC | 3 cut(s) | 1330, 3989, 7116 |
| FriOI | GRGCYC | 6 cut(s) | 269, 2398, 4088, 4483, 4814, 7175 |
| FspI | TGCGCA | 1 cut(s) | 236 |
| GlaI | GCGC | 6 cut(s) | 236, 4434, 5432, 6453, 6600, 6755 |
| GsaI | CCCAGC | 2 cut(s) | 3878, 7442 |
| GsuI | CTGGAG | 8 cut(s) | 2104, 2311, 4200, 4269, 4446, 4932, 6054, 6564 |
| HaeII | RGCGCY | 2 cut(s) | 4436, 6455 |
| HgaI | GACGC | 2 cut(s) | 2780, 4617 |
| HhaI | GCGC | 6 cut(s) | 237, 4435, 5433, 6454, 6601, 6756 |
| Hin6I | GCGC | 6 cut(s) | 235, 4433, 5431, 6452, 6599, 6754 |
| HinP1I | GCGC | 6 cut(s) | 235, 4433, 5431, 6452, 6599, 6754 |
| HincII | GTYRAC | 2 cut(s) | 715, 5446 |
| HindII | GTYRAC | 2 cut(s) | 715, 5446 |
| HindIII | AAGCTT | 5 cut(s) | 197, 951, 2000, 2326, 3824 |
| Hpy99I | CGWCG | 3 cut(s) | 747, 3341, 4611 |
| HpyCH4IV | ACGT | 8 cut(s) | 1390, 3676, 4151, 4689, 5248, 5729, 6843, 7467 |
| HpySE526I | ACGT | 8 cut(s) | 1390, 3676, 4151, 4689, 5248, 5729, 6843, 7467 |
| HspAI | GCGC | 6 cut(s) | 235, 4433, 5431, 6452, 6599, 6754 |
| KpnI | GGTACC | 3 cut(s) | 3476, 7233, 7399 |
| Ksp22I | TGATCA | 4 cut(s) | 385, 6043, 6166, 7216 |
| LguI | GCTCTTC | 2 cut(s) | 1906, 1914 |
| MabI | ACCWGGT | 1 cut(s) | 6553 |
| MaeII | ACGT | 8 cut(s) | 1390, 3676, 4151, 4689, 5248, 5729, 6843, 7467 |
| MbiI | CCGCTC | 1 cut(s) | 5760 |
| MfeI | CAATTG | 3 cut(s) | 728, 5316, 5439 |
| MflI | RGATCY | 6 cut(s) | 1564, 1609, 3362, 4322, 5360, 6226 |
| MhlI | GDGCHC | 8 cut(s) | 269, 2398, 3532, 4088, 4483, 4814, 6442, 7175 |
| MlsI | TGGCCA | 4 cut(s) | 2355, 6262, 6833, 7317 |
| MluNI | TGGCCA | 4 cut(s) | 2355, 6262, 6833, 7317 |
| Mox20I | TGGCCA | 4 cut(s) | 2355, 6262, 6833, 7317 |
| Mph1103I | ATGCAT | 2 cut(s) | 3348, 5494 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1060, 2411, 4376 |
| MscI | TGGCCA | 4 cut(s) | 2355, 6262, 6833, 7317 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1044, 2481, 2625, 3687, 5039 |
| Msp20I | TGGCCA | 4 cut(s) | 2355, 6262, 6833, 7317 |
| MspA1I | CMGCKG | 8 cut(s) | 141, 3665, 5276, 5927, 5936, 6032, 6101, 6542 |
| MunI | CAATTG | 3 cut(s) | 728, 5316, 5439 |
| Mva1269I | GAATGC | 3 cut(s) | 1815, 3178, 3423 |
| MvnI | CGCG | 1 cut(s) | 7027 |
| NciI | CCSGG | 3 cut(s) | 12, 1607, 6484 |
| NcoI | CCATGG | 5 cut(s) | 2626, 4483, 6702, 6816, 6979 |
| NdeI | CATATG | 2 cut(s) | 55, 3657 |
| NheI | GCTAGC | 1 cut(s) | 1783 |
| NmeAIII | GCCGAG | 2 cut(s) | 466, 2162 |
| NsbI | TGCGCA | 1 cut(s) | 236 |
| NsiI | ATGCAT | 2 cut(s) | 3348, 5494 |
| NspI | RCATGY | 9 cut(s) | 721, 1988, 2624, 3346, 3686, 3949, 5629, 5809, 6772 |
| PaeI | GCATGC | 2 cut(s) | 1988, 5629 |
| PaeR7I | CTCGAG | 1 cut(s) | 3060 |
| PagI | TCATGA | 2 cut(s) | 3040, 3532 |
| PaqCI | CACCTGC | 1 cut(s) | 1463 |
| PasI | CCCWGGG | 2 cut(s) | 4006, 6797 |
| PceI | AGGCCT | 3 cut(s) | 166, 316, 610 |
| PciI | ACATGT | 1 cut(s) | 3682 |
| PciSI | GCTCTTC | 2 cut(s) | 1906, 1914 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 5134 |
| PctI | GAATGC | 3 cut(s) | 1815, 3178, 3423 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1060, 2411, 4376 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 1169, 4496, 6155, 6790 |
| PfoI | TCCNGGA | 3 cut(s) | 10, 2288, 3151 |
| PinAI | ACCGGT | 1 cut(s) | 1395 |
| Ppu21I | YACGTR | 1 cut(s) | 7468 |
| PpuMI | RGGWCCY | 6 cut(s) | 503, 2211, 3103, 3815, 4025, 6368 |
| PscI | ACATGT | 1 cut(s) | 3682 |
| PshAI | GACNNNNGTC | 1 cut(s) | 7050 |
| PshBI | ATTAAT | 1 cut(s) | 2916 |
| Psp124BI | GAGCTC | 2 cut(s) | 2398, 7175 |
| Psp5II | RGGWCCY | 6 cut(s) | 503, 2211, 3103, 3815, 4025, 6368 |
| PspEI | GGTNACC | 1 cut(s) | 6801 |
| PspFI | CCCAGC | 2 cut(s) | 3874, 7438 |
| PspOMI | GGGCCC | 1 cut(s) | 4479 |
| PspPPI | RGGWCCY | 6 cut(s) | 503, 2211, 3103, 3815, 4025, 6368 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 3780, 3812 |
| PstI | CTGCAG | 3 cut(s) | 301, 1474, 5833 |
| PstNI | CAGNNNCTG | 5 cut(s) | 4562, 4673, 5918, 6032, 7417 |
| PsuI | RGATCY | 6 cut(s) | 1564, 1609, 3362, 4322, 5360, 6226 |
| PvuII | CAGCTG | 6 cut(s) | 3665, 5276, 5927, 5936, 6032, 6542 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1044, 2481, 2625, 3687, 5039 |
| SacI | GAGCTC | 2 cut(s) | 2398, 7175 |
| SapI | GCTCTTC | 2 cut(s) | 1906, 1914 |
| ScaI | AGTACT | 4 cut(s) | 694, 3700, 4014, 5158 |
| SduI | GDGCHC | 8 cut(s) | 269, 2398, 3532, 4088, 4483, 4814, 6442, 7175 |
| SexAI | ACCWGGT | 1 cut(s) | 6553 |
| SfcI | CTRYAG | 9 cut(s) | 297, 1350, 1470, 2773, 3783, 5400, 5829, 6603, 7065 |
| Sfr274I | CTCGAG | 1 cut(s) | 3060 |
| SlaI | CTCGAG | 1 cut(s) | 3060 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1044, 2481, 2625, 3687, 5039 |
| SpeI | ACTAGT | 2 cut(s) | 850, 6434 |
| SphI | GCATGC | 2 cut(s) | 1988, 5629 |
| SseBI | AGGCCT | 3 cut(s) | 166, 316, 610 |
| SspI | AATATT | 3 cut(s) | 2896, 3316, 4936 |
| SstI | GAGCTC | 2 cut(s) | 2398, 7175 |
| StuI | AGGCCT | 3 cut(s) | 166, 316, 610 |
| TaiI | ACGT | 8 cut(s) | 1393, 3679, 4154, 4692, 5251, 5732, 6846, 7470 |
| TaqII | GACCGA | 2 cut(s) | 1212, 5787 |
| TatI | WGTACW | 6 cut(s) | 692, 751, 2829, 3698, 4012, 5156 |
| TauI | GCSGC | 2 cut(s) | 724, 5760 |
| TspGWI | ACGGA | 4 cut(s) | 650, 2269, 3921, 5521 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 1169, 4496, 6155, 6790 |
| VneI | GTGCAC | 1 cut(s) | 6438 |
| VspI | ATTAAT | 1 cut(s) | 2916 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2698, 2773 |
| XapI | RAATTY | 9 cut(s) | 2567, 2668, 3522, 3625, 4234, 4534, 6050, 6283, 6971 |
| XbaI | TCTAGA | 3 cut(s) | 2243, 2812, 4544 |
| XceI | RCATGY | 9 cut(s) | 721, 1988, 2624, 3346, 3686, 3949, 5629, 5809, 6772 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 419, 873, 6255, 6462 |
| XhoI | CTCGAG | 1 cut(s) | 3060 |
| XmaJI | CCTAGG | 2 cut(s) | 3388, 4751 |
| XmiI | GTMKAC | 3 cut(s) | 1330, 3989, 7116 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1060, 2411, 4376 |
| ZrmI | AGTACT | 4 cut(s) | 694, 3700, 4014, 5158 |
| Zsp2I | ATGCAT | 2 cut(s) | 3348, 5494 |