MD15G1307000.v1.1

Nodulation receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013594
Physical Location & Seq
Forward (+)
30508935 .. 30516158
7224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1307000.v1.1.491

Sequence Viewer

Length: 2085 bp
ATGATGAAGGAATTTGATTATCGGGTTTTAAGACTAATCAAGTTTTCGATCATCTGCCTCTTCATTCTTGTTCCATCCACTTCTGCAGATGAAGGCAGATTTGTGAGCATTGCGTGCTGCGGGGAATCGAATTACACGGATAGCAGAAACATTAGTTGGATATCGGACTATGAACAGTTTCCTAATACCACAAGCTGTTACACAAGAGCTCAGTCAGAGGTAGACTACTCCGGTTACAACAGAAGTAGAGTATTTTACCAGAATTCAGGCCAGAAAAGATGTTACAAGCTATCAACAGAGAAGGATGAAGACTATCTGATTAGGGCTACATTTCTATTTGGACACATTTGGGGCTCTAGTTTTTCGGTTTGGTGTGACCCCGATTGCTGTCGTAGATTCGTCGGAGGACTTGTCGGTGCTCCTTATATTTCAGAGCTTGAGCTGAGGCTTCTGCAGGATTTGAAATACTATTTAGAGAACTTCCCTTCAAGTGTTCTGAAATTGGTTACAAGAAATAGTTTTGGAATTACAAAAGATGATATCAGGTATCCAAATGACACAAATGACAGAATATGGAAATCAAATTCAAGTGCCGACCCAGGATGCGGAACCTATCACACATCACAACCCAACGTCTCCATTTCCGGCAACGCCACCGCGTCTCCGCCTCTGCAAGTCCTGCAAACAGCTCTAGCGGCTACAGACAGTTTGCGTTTTCTCCACAATGGCCTCCCCACAGAAAGTTACCAGTACCTCGTGTTCCTCTACATCTACGAGGTCGATCGGACGGTCCAAACCGGCCAAAGGGTGTTCGATATTTTCCTCAACGATGAGATAAAAGAAGGGAGATTTGACGTCTCGGGAAATGGGTCCAATTACAAGGAGCTTGCTTTCACTGTCATGACAAATGGGTCCCTGAATTTGACTTTGGTTAAGGTCTCCGGGTCGGAAAATGGACCCATTTGCAATGCTTATGAGATCTTGCAGGTGCTCCCGTGGCGTCCAGAGACTACTAAAAGTGATGTGGAAGTGATTTTGGAGGTGAAGAATGAGATGCTGAAGAATAACCACGAGATTGAAGTTTGGGAGGGTTGGGCTGGAGACCCGTGTCTACCAGTTCCATGGGATGGTATTACATGTGAATCCATTGATGGGTACAGTATCATTACTGAACTGGATCTTTCGTCAGGCATTCAGAGGGGGTTTGATCATAAAAGCTTTCTTAAAGGGCCAGTTCCTTCAAGTATCACTAAGCTGAGCCACCTAAAAGTCCTGAACCTCAGCCACAATGACTTTACTGGCAACATTCCAGCATTTCTTCCTTCCTCCCTGCTGACCTCAGTGGATCTGAGCTTCAATGACCTTTGGGGGTCACTTCCATTATCTCTTATCTCACTGCCACATTTGGATATACTGTGCAACCCTCATGTTGCAAAAGATATTCCCTCCAGTTTCAATGGCTCAAAACTTACCACGGGCCAAGAAAGTTGCAATGCTCAAGAAGCTTCTAATCAACGAATCATCATTGGTGCTGCTGCTGCATCTGGGTTTGTACTACTAACCATTGTAGTTGGGATAATTTTCTGCACTTGGAGACAAAACTTTCTACCTCAAGGAAATTTTGATATAAAAAGACATCATCATATGACAAAGAATTTAATTTTCTCGTTACCAAGCATGGACGATCTGGTTATGAAGTCAATATCCATAGAGACATTTACTCTTGAGTACATAGAGGCTGCTACCCAAAGGTACAAGACCTTGATAGGTGAAGGAGGTTTTGGGTCTGTTTTCCATGGCACTTTAATTGATGGTCAGGAAGTGGCAGTAAAGGTTCGGTCAGCAACATCAACCCAAGGAACTCGGGAATTTGAGAATGAGCTAAATCTTCTGTCAGCTATACGCCATGAGAACCTCATGCCCCTTCTCGGTTATTGTTGTGAGAATGACCAAGAAATTCTGGTTTATCTATTTATGTCCAACGGCACTTTACAGGATTGTCTCTATGGGGAAGCAGCAAAAAGAAAAATTCTGGACTGGCCAACCAGACTGTCAATTACTCTTGGTGCTGCTCGAGGCAAGTGA

Protein Analysis

695

Amino Acids

77.68

Weight (kDa)

5.33

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 41 - 115 6.1e-08 Malectin-like domain
Malectin_like PF12819 139 - 330 5e-29 Malectin-like domain
PK_Tyr_Ser-Thr PF07714 586 - 693 2.6e-14 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 586 - 669 3.6e-08 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 976
AasI GACNNNNNNGTC 1 cut(s) 910
AatII GACGTC 1 cut(s) 858
Acc36I ACCTGC 1 cut(s) 976
AccB7I CCANNNNNTGG 1 cut(s) 1127
AccI GTMKAC 2 cut(s) 222, 1111
AccII CGCG 1 cut(s) 659
AciI CCGC 5 cut(s) 120, 606, 657, 665, 695
AclWI GGATC 2 cut(s) 1185, 1353
AcoI YGGCCR 2 cut(s) 799, 2039
AcsI RAATTY 9 cut(s) 11, 262, 583, 919, 1618, 1654, 1868, 1956, 2028
AcuI CTGAAG 1 cut(s) 1079
AcyI GRCGYC 2 cut(s) 855, 1000
AfaI GTAC 5 cut(s) 752, 1157, 1554, 1730, 1754
AfiI CCNNNNNNNGG 5 cut(s) 605, 804, 1127, 1152, 1928
AflIII ACRYGT 1 cut(s) 1136
AgsI TTSAA 7 cut(s) 463, 489, 588, 1079, 1242, 1357, 1456
AjnI CCWGG 1 cut(s) 598
AjuI GAANNNNNNNTTGG 6 cut(s) 139, 171, 911, 943, 1764, 1796
Alw21I GWGCWC 3 cut(s) 211, 421, 993
Alw26I GTCTC 9 cut(s) 640, 666, 862, 943, 1001, 1095, 1588, 1706, 2006
AlwI GGATC 2 cut(s) 1185, 1353
Ama87I CYCGRG 3 cut(s) 859, 1863, 2073
AoxI GGCC 6 cut(s) 268, 727, 799, 1229, 1477, 2039
ApeKI GCWGC 7 cut(s) 117, 1532, 1535, 1538, 1739, 2015, 2069
ApoI RAATTY 9 cut(s) 11, 262, 583, 919, 1618, 1654, 1868, 1956, 2028
Asp700I GAANNNNTTC 1 cut(s) 177
AspS9I GGNCC 6 cut(s) 790, 870, 912, 956, 1229, 1477
AsuC2I CCSGG 1 cut(s) 943
AsuHPI GGTGA 2 cut(s) 1054, 1781
AvaI CYCGRG 3 cut(s) 859, 1863, 2073
AvaII GGWCC 4 cut(s) 790, 870, 912, 956
BalI TGGCCA 1 cut(s) 2041
BanII GRGCYC 2 cut(s) 211, 356
BauI CACGAG 2 cut(s) 755, 1070
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 3 cut(s) 211, 421, 993
BbvCI CCTCAGC 2 cut(s) 443, 1280
BbvI GCAGC 7 cut(s) 104, 1519, 1522, 1525, 1726, 2027, 2056
BccI CCATC 4 cut(s) 82, 1121, 1145, 1805
BceAI ACGGC 1 cut(s) 1999
BciT130I CCWGG 1 cut(s) 600
BciVI GTATCC 1 cut(s) 558
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 1 cut(s) 943
BcoDI GTCTC 9 cut(s) 640, 666, 862, 943, 1001, 1095, 1588, 1706, 2006
BfaI CTAG 2 cut(s) 357, 692
BfmI CTRYAG 3 cut(s) 84, 452, 699
BfuAI ACCTGC 1 cut(s) 976
BfuI GTATCC 1 cut(s) 558
BglII AGATCT 1 cut(s) 978
BisI GCNGC 8 cut(s) 118, 696, 1533, 1536, 1539, 1740, 2016, 2070
BlpI GCTNAGC 1 cut(s) 1256
BlsI GCNGC 8 cut(s) 119, 697, 1534, 1537, 1540, 1741, 2017, 2071
Bme1390I CCNGG 2 cut(s) 600, 943
Bme18I GGWCC 4 cut(s) 790, 870, 912, 956
BmeT110I CYCGRG 3 cut(s) 859, 1863, 2073
BmgT120I GGNCC 6 cut(s) 790, 870, 912, 956, 1229, 1477
BmiI GGNNCC 5 cut(s) 610, 871, 913, 914, 958
BmrFI CCNGG 2 cut(s) 600, 943
BmsI GCATC 3 cut(s) 593, 1044, 1550
BoxI GACNNNNGTC 1 cut(s) 1107
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 2 cut(s) 1119, 1432
Bpu10I CCTNAGC 2 cut(s) 443, 1280
Bpu1102I GCTNAGC 1 cut(s) 1256
BpuEI CTTGAG 4 cut(s) 458, 1482, 1596, 1745
BpuMI CCSGG 1 cut(s) 943
BsaHI GRCGYC 2 cut(s) 855, 1000
BsaI GGTCTC 2 cut(s) 943, 1095
BsaJI CCNNGG 6 cut(s) 598, 995, 1121, 1473, 1795, 1855
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 4 cut(s) 396, 426, 646, 676
Bsc4I CCNNNNNNNGG 5 cut(s) 605, 804, 1127, 1152, 1928
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 7 cut(s) 748, 1115, 1179, 1232, 1303, 1449, 2042
Bse3DI GCAATG 3 cut(s) 108, 973, 1498
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 6 cut(s) 598, 995, 1121, 1473, 1795, 1855
BseGI GGATG 4 cut(s) 74, 310, 608, 1132
BseLI CCNNNNNNNGG 5 cut(s) 605, 804, 1127, 1152, 1928
BseMI GCAATG 3 cut(s) 108, 973, 1498
BseMII CTCAG 6 cut(s) 224, 434, 1247, 1294, 1340, 1353
BseNI ACTGG 7 cut(s) 748, 1115, 1179, 1232, 1303, 1449, 2042
BseXI GCAGC 7 cut(s) 104, 1519, 1522, 1525, 1726, 2027, 2056
BsgI GTGCAG 1 cut(s) 1570
Bsh1236I CGCG 1 cut(s) 659
Bsh1285I CGRYCG 1 cut(s) 784
BshFI GGCC 6 cut(s) 270, 729, 801, 1231, 1479, 2041
BsiEI CGRYCG 1 cut(s) 784
BsiHKAI GWGCWC 3 cut(s) 211, 421, 993
BsiHKCI CYCGRG 3 cut(s) 859, 1863, 2073
BsiSI CCGG 4 cut(s) 231, 645, 798, 942
BslFI GGGAC 1 cut(s) 898
BslI CCNNNNNNNGG 5 cut(s) 605, 804, 1127, 1152, 1928
BsmAI GTCTC 9 cut(s) 640, 666, 862, 943, 1001, 1095, 1588, 1706, 2006
BsmBI CGTCTC 3 cut(s) 640, 666, 862
BsmFI GGGAC 1 cut(s) 898
BsmI GAATGC 1 cut(s) 1191
BsnI GGCC 6 cut(s) 270, 729, 801, 1231, 1479, 2041
Bso31I GGTCTC 2 cut(s) 943, 1095
BsoBI CYCGRG 3 cut(s) 859, 1863, 2073
Bsp1286I GDGCHC 4 cut(s) 211, 356, 421, 993
Bsp143I GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
Bsp1720I GCTNAGC 1 cut(s) 1256
Bsp19I CCATGG 2 cut(s) 1121, 1795
BspACI CCGC 5 cut(s) 120, 606, 657, 665, 695
BspANI GGCC 6 cut(s) 270, 729, 801, 1231, 1479, 2041
BspCNI CTCAG 6 cut(s) 223, 435, 1248, 1293, 1341, 1352
BspFNI CGCG 1 cut(s) 659
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 5 cut(s) 610, 871, 913, 914, 958
BspMAI CTGCAG 2 cut(s) 88, 456
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 2 cut(s) 1185, 1353
BspTNI GGTCTC 2 cut(s) 943, 1095
BsrDI GCAATG 3 cut(s) 108, 973, 1498
BsrFI RCCGGY 1 cut(s) 797
BsrI ACTGG 7 cut(s) 748, 1115, 1179, 1232, 1303, 1449, 2042
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 6 cut(s) 598, 995, 1121, 1473, 1795, 1855
BssMI GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
BssNI GRCGYC 2 cut(s) 855, 1000
BssSI CACGAG 2 cut(s) 755, 1070
BssT1I CCWWGG 3 cut(s) 1121, 1795, 1855
Bst2BI CACGAG 2 cut(s) 755, 1070
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 7 cut(s) 177, 707, 790, 898, 1160, 1416, 2052
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 2 cut(s) 855, 1000
BstAPI GCANNNNNTGC 2 cut(s) 114, 679
BstC8I GCNNGC 2 cut(s) 115, 888
BstDEI CTNAG 7 cut(s) 210, 443, 1251, 1256, 1280, 1339, 1349
BstDSI CCRYGG 4 cut(s) 995, 1121, 1473, 1795
BstF5I GGATG 4 cut(s) 74, 310, 608, 1132
BstFNI CGCG 1 cut(s) 659
BstKTI GATC 7 cut(s) 51, 784, 981, 1180, 1210, 1348, 1687
BstMAI GTCTC 9 cut(s) 640, 666, 862, 943, 1001, 1095, 1588, 1706, 2006
BstMBI GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
BstMCI CGRYCG 1 cut(s) 784
BstMWI GCNNNNNNNGC 6 cut(s) 114, 679, 695, 997, 1502, 1538
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 1 cut(s) 1140
BstPAI GACNNNNGTC 1 cut(s) 1107
BstSCI CCNGG 2 cut(s) 598, 941
BstSFI CTRYAG 3 cut(s) 84, 452, 699
BstUI CGCG 1 cut(s) 659
BstV1I GCAGC 7 cut(s) 104, 1519, 1522, 1525, 1726, 2027, 2056
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 3 cut(s) 978, 1177, 1345
BstXI CCANNNNNNTGG 1 cut(s) 1122
BstYI RGATCY 3 cut(s) 978, 1177, 1345
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 6 cut(s) 270, 729, 801, 1231, 1479, 2041
BtgI CCRYGG 4 cut(s) 995, 1121, 1473, 1795
BtsCI GGATG 4 cut(s) 74, 310, 608, 1132
BtsI GCAGTG 1 cut(s) 1394
BtsIMutI CAGTG 3 cut(s) 894, 1347, 1394
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 2 cut(s) 115, 888
CciI TCATGA 1 cut(s) 900
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 6 cut(s) 790, 870, 912, 956, 1229, 1477
CseI GACGC 2 cut(s) 648, 989
Csp6I GTAC 5 cut(s) 751, 1156, 1553, 1729, 1753
CviAII CATG 8 cut(s) 901, 1122, 1137, 1427, 1678, 1796, 1907, 1918
CviQI GTAC 5 cut(s) 751, 1156, 1553, 1729, 1753
DdeI CTNAG 7 cut(s) 210, 443, 1251, 1256, 1280, 1339, 1349
DpnI GATC 7 cut(s) 50, 783, 980, 1179, 1209, 1347, 1686
DpnII GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
DrdI GACNNNNNNGTC 1 cut(s) 910
DseDI GACNNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 2 cut(s) 799, 2039
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 654
Ecl136II GAGCTC 1 cut(s) 209
Eco130I CCWWGG 3 cut(s) 1121, 1795, 1855
Eco24I GRGCYC 2 cut(s) 211, 356
Eco31I GGTCTC 2 cut(s) 943, 1095
Eco32I GATATC 2 cut(s) 162, 541
Eco47I GGWCC 4 cut(s) 790, 870, 912, 956
Eco53kI GAGCTC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 1079
Eco88I CYCGRG 3 cut(s) 859, 1863, 2073
EcoICRI GAGCTC 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 912
EcoRI GAATTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 598
EcoRV GATATC 2 cut(s) 162, 541
EcoT14I CCWWGG 3 cut(s) 1121, 1795, 1855
EcoT38I GRGCYC 2 cut(s) 211, 356
ErhI CCWWGG 3 cut(s) 1121, 1795, 1855
Esp3I CGTCTC 3 cut(s) 640, 666, 862
FaeI CATG 8 cut(s) 904, 1125, 1140, 1430, 1681, 1799, 1910, 1921
FaqI GGGAC 1 cut(s) 898
FatI CATG 8 cut(s) 900, 1121, 1136, 1426, 1677, 1795, 1906, 1917
FauI CCCGC 1 cut(s) 113
FauNDI CATATG 1 cut(s) 1644
FbaI TGATCA 1 cut(s) 1207
FblI GTMKAC 2 cut(s) 222, 1111
Fnu4HI GCNGC 8 cut(s) 118, 696, 1533, 1536, 1539, 1740, 2016, 2070
FokI GGATG 4 cut(s) 61, 317, 615, 1139
FriOI GRGCYC 2 cut(s) 211, 356
Fsp4HI GCNGC 8 cut(s) 118, 696, 1533, 1536, 1539, 1740, 2016, 2070
FspBI CTAG 2 cut(s) 357, 692
GluI GCNGC 8 cut(s) 118, 696, 1533, 1536, 1539, 1740, 2016, 2070
GsuI CTGGAG 2 cut(s) 1119, 1432
HaeIII GGCC 6 cut(s) 270, 729, 801, 1231, 1479, 2041
HapII CCGG 4 cut(s) 231, 645, 798, 942
HgaI GACGC 2 cut(s) 648, 989
Hin1I GRCGYC 2 cut(s) 855, 1000
Hin1II CATG 8 cut(s) 904, 1125, 1140, 1430, 1681, 1799, 1910, 1921
HindIII AAGCTT 2 cut(s) 1216, 1503
HinfI GANTC 4 cut(s) 125, 396, 1142, 1518
HpaII CCGG 4 cut(s) 231, 645, 798, 942
HphI GGTGA 2 cut(s) 1054, 1781
Hpy166II GTNNAC 2 cut(s) 223, 1112
Hpy188III TCNNGA 9 cut(s) 861, 901, 1004, 1273, 1499, 1724, 1817, 1865, 2033
Hpy8I GTNNAC 2 cut(s) 223, 1112
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 8 cut(s) 86, 295, 495, 836, 1248, 1332, 1766, 1934
HpyCH4III ACNGT 7 cut(s) 177, 707, 790, 898, 1160, 1416, 2052
HpyCH4IV ACGT 2 cut(s) 633, 855
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 679, 695, 997, 1502, 1538
HpyF3I CTNAG 7 cut(s) 210, 443, 1251, 1256, 1280, 1339, 1349
HpySE526I ACGT 2 cut(s) 633, 855
Hsp92I GRCGYC 2 cut(s) 855, 1000
Hsp92II CATG 8 cut(s) 904, 1125, 1140, 1430, 1681, 1799, 1910, 1921
KflI GGGWCCC 1 cut(s) 912
Ksp22I TGATCA 1 cut(s) 1207
Kzo9I GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
LmnI GCTCC 3 cut(s) 424, 883, 996
Lsp1109I GCAGC 7 cut(s) 104, 1519, 1522, 1525, 1726, 2027, 2056
LweI GCATC 3 cut(s) 593, 1044, 1550
MaeI CTAG 2 cut(s) 357, 692
MaeII ACGT 2 cut(s) 633, 855
MaeIII GTNAC 8 cut(s) 197, 233, 281, 374, 505, 743, 1371, 1668
MalI GATC 7 cut(s) 50, 783, 980, 1179, 1209, 1347, 1686
MboI GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
MboII GAAGA 6 cut(s) 52, 320, 1057, 1072, 1310, 1880
MflI RGATCY 3 cut(s) 978, 1177, 1345
MhlI GDGCHC 4 cut(s) 211, 356, 421, 993
MlsI TGGCCA 1 cut(s) 2041
MluNI TGGCCA 1 cut(s) 2041
MmeI TCCRAC 4 cut(s) 137, 382, 927, 2004
Mox20I TGGCCA 1 cut(s) 2041
MroXI GAANNNNTTC 1 cut(s) 177
MscI TGGCCA 1 cut(s) 2041
MseI TTAA 5 cut(s) 29, 933, 1224, 1658, 1805
MslI CAYNNNNRTG 1 cut(s) 899
Msp20I TGGCCA 1 cut(s) 2041
MspI CCGG 4 cut(s) 231, 645, 798, 942
MspR9I CCNGG 2 cut(s) 600, 943
Mva1269I GAATGC 1 cut(s) 1191
MvaI CCWGG 1 cut(s) 600
MvnI CGCG 1 cut(s) 659
MwoI GCNNNNNNNGC 6 cut(s) 114, 679, 695, 997, 1502, 1538
NciI CCSGG 1 cut(s) 943
NcoI CCATGG 2 cut(s) 1121, 1795
NdeI CATATG 1 cut(s) 1644
NdeII GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
NlaIII CATG 8 cut(s) 904, 1125, 1140, 1430, 1681, 1799, 1910, 1921
NlaIV GGNNCC 5 cut(s) 610, 871, 913, 914, 958
NmuCI GTSAC 2 cut(s) 374, 1371
NspI RCATGY 1 cut(s) 1140
PaeR7I CTCGAG 1 cut(s) 2073
PagI TCATGA 1 cut(s) 900
PaqCI CACCTGC 1 cut(s) 976
PciI ACATGT 1 cut(s) 1136
PctI GAATGC 1 cut(s) 1191
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 4 cut(s) 125, 396, 1142, 1518
PflMI CCANNNNNTGG 1 cut(s) 1127
PkrI GCNGC 8 cut(s) 119, 697, 1534, 1537, 1540, 1741, 2017, 2071
Ple19I CGATCG 1 cut(s) 784
PpuMI RGGWCCY 1 cut(s) 912
PscI ACATGT 1 cut(s) 1136
PshAI GACNNNNGTC 1 cut(s) 1107
Psp124BI GAGCTC 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 912
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 5 cut(s) 610, 871, 913, 914, 958
PspPI GGNCC 6 cut(s) 790, 870, 912, 956, 1229, 1477
PspPPI RGGWCCY 1 cut(s) 912
PspXI VCTCGAGB 1 cut(s) 2073
PsrI GAACNNNNNNTAC 2 cut(s) 743, 775
PstI CTGCAG 2 cut(s) 88, 456
PsuI RGATCY 3 cut(s) 978, 1177, 1345
PvuI CGATCG 1 cut(s) 784
RsaI GTAC 5 cut(s) 752, 1157, 1554, 1730, 1754
RsaNI GTAC 5 cut(s) 751, 1156, 1553, 1729, 1753
RseI CAYNNNNRTG 1 cut(s) 899
SacI GAGCTC 1 cut(s) 211
SaqAI TTAA 5 cut(s) 29, 933, 1224, 1658, 1805
SatI GCNGC 8 cut(s) 118, 696, 1533, 1536, 1539, 1740, 2016, 2070
Sau3AI GATC 7 cut(s) 48, 781, 978, 1177, 1207, 1345, 1684
Sau96I GGNCC 6 cut(s) 790, 870, 912, 956, 1229, 1477
ScrFI CCNGG 2 cut(s) 600, 943
SduI GDGCHC 4 cut(s) 211, 356, 421, 993
SfaNI GCATC 3 cut(s) 593, 1044, 1550
SfcI CTRYAG 3 cut(s) 84, 452, 699
Sfr274I CTCGAG 1 cut(s) 2073
SinI GGWCC 4 cut(s) 790, 870, 912, 956
SlaI CTCGAG 1 cut(s) 2073
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 5 cut(s) 437, 1497, 1611, 1724, 2073
SmoI CTYRAG 5 cut(s) 437, 1497, 1611, 1724, 2073
SsiI CCGC 5 cut(s) 120, 606, 657, 665, 695
SspMI CTAG 2 cut(s) 357, 692
SstI GAGCTC 1 cut(s) 211
StyD4I CCNGG 2 cut(s) 598, 941
StyI CCWWGG 3 cut(s) 1121, 1795, 1855
TaaI ACNGT 7 cut(s) 177, 707, 790, 898, 1160, 1416, 2052
TaiI ACGT 2 cut(s) 636, 858
TaqI TCGA 5 cut(s) 47, 128, 780, 813, 2074
TaqII GACCGA 1 cut(s) 1827
TatI WGTACW 2 cut(s) 1552, 1728
TauI GCSGC 1 cut(s) 698
TfiI GAWTC 4 cut(s) 125, 396, 1142, 1518
Tru1I TTAA 5 cut(s) 29, 933, 1224, 1658, 1805
Tru9I TTAA 5 cut(s) 29, 933, 1224, 1658, 1805
TscAI CASTG 3 cut(s) 901, 1347, 1401
TseFI GTSAC 2 cut(s) 374, 1371
TseI GCWGC 7 cut(s) 117, 1532, 1535, 1538, 1739, 2015, 2069
Tsp45I GTSAC 2 cut(s) 374, 1371
TspDTI ATGAA 6 cut(s) 20, 52, 105, 186, 321, 1709
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 3 cut(s) 901, 1347, 1401
Van91I CCANNNNNTGG 1 cut(s) 1127
VpaK11BI GGWCC 4 cut(s) 790, 870, 912, 956
XapI RAATTY 9 cut(s) 11, 262, 583, 919, 1618, 1654, 1868, 1956, 2028
XceI RCATGY 1 cut(s) 1140
XhoI CTCGAG 1 cut(s) 2073
XmiI GTMKAC 2 cut(s) 222, 1111
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 2 cut(s) 357, 692
ZraI GACGTC 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.