Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0013594

Physical Location & Seq

Genomic Coordinates Forward (+)

21134956 .. 21142257

Gene Structure 7302 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017781

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2811 bp

ATGAAGCAATTAAACATTTGGGTTTTAAGACTGACAGAGTTCTTCATACTTTGCCTCTTCATTTTTAATCCATTGACTTCTGCAGCTGAAGGCAGTAATTCAAGCAGCTTTGTGAGTATTGCATGCTGCTCAGAATCTAATTTTACAGATAGAAGAAACATAAGTTGGGTAACAGACTTTAATGAGTTTCGTAATAGGCTAAGTTGTGAGGACAGAAAGCCAATTGCGATTGATTATGTTAACAATAAAACAAGGGTGTTTCACGTTGGTCCAAATCAGAAAAGATGTTATATCTTACCATCAATAAAGGGTGAAGACTATCTAATAAGGGGTATTTTTGGAGGCATCTGGGGAGTGAAATTTACAGTTGCCATTGGTGTGACCACAATCGGAGTAGTAGATACATCCGATGATTTAGCGGTTGAGGGAGTCTTTAGAGCCACCAAGGACTACACACACTTTTGCTTGGTGAGTGACGAAGGAGCACCTTATATTTCAGAGATTCAGTTGAGGCCTCTGTGGAACTTGGGTTACTATCTAGAGGACTTCTCTTCAAGTGTTCTGGAACTGCTTAAAAGGACTAGTTTTGATGATATAAGAGCAGAAAAGTTCATCAGGTACGAAGCTGACCCGAGTGACAGAATCTGGTTAACAAACTCAACTGTCGACCTGGGAACCTTCCACATAACAGAACCCAATGTCAACGTTCTGGGCGTCACAGATGTTCGAGTACCCATTCAAGTCCTCCATACAGCTCTAGCTTCTTCAGACCGCTTGGTCATTCTTCAGGATGGCCTGTCCTCAACAAACAATCACTACCTTTTGTTCCTGTACTTCTATGAAGTCAATCAAACTGTTCAAGCTGGGAAGAGGGTTTTTGATGTTTACTTGAACAGTGAGCTGAGACGGCAAGGATTTGATGTTATGGGTAATGGGTCAAATTACAGGGAGCTTGCTTTGAATGTGACAGCAAATGGGTATCTGAATCTGACTCTGGTTAAGGCGTCTGAGTCTGAAAATGGACCCATTTGCAATGCCTATGAGATTTTGCAGGTGCGCCAGTTGGTTCAGGAGACTAACACAGACGATGTGGAAGTGATTTTGGAGGTGAGGAGTGAGTTGCTGGAGAATAATCAGATGAATGAAGTTTGGAAAGGGTGGACTGCAGACCCATGTCTTCCTGTTCCCTGGAATGGTATAGGTTGTGAAACTGTTAATAGTGTCACTGTCATTACCAAACTGGATCTTTCTTCTGGCATTCAGAGAGGGTCTGATGAAGAGCGGAAACTTATAGGACCATTTCCTAAAAGCATCACTAAGCTGACTCACCTAAAATTCCTGAACCTCAGCAACAACGGGTTTACTGACCAAATTCCAGAATTTCTTGAACGTACCATGTTGATTTCAGTGGATCTGAGCCATAATAATCTTTCGGGGCCACTTCCAAATTCTCTTGCCTCACAACCAAATTTGGAAACACTAAATTTTGGCTGCAATCCTCAGCTTACCAAAGTTAGTCCATCAACCTTCAGTGGCTTAAAATTTACAACAGACCATGGAAATTGTGGTGTGACTGGTGTCCAAAAATTCTCGAAACGAGGAATCATCATTAGTGCTGCAGCTGCATCTGGATCTTTACTAGTAACAGCTGTAGCCGGGATTATTTTTTTCTTGAGGAGACAAAAGCTTTTGCCTCAAGGAAAATTTGATAAGCAACCGTATCCTATGGCAAAGAATTTAATATTCTCATTACCTAGCATGGATGATCTGATCATGAAATCAATAGCCATAGAGACATTTACTCTTGAGTACCTAGAGGCTGTCACCCAAAGTTACAAAACGCTGATAGGCGAAGGAGGTTTTGGGTCCGTTTACCGTGGCACTTTAACTGATGGTCAGGAAGTGGCAGTGAAGGTTCGATCAGCAACATCAACCCAAGGGACTCGGGAATTTGATAATGAGCTAAATCTTCTGTCAACTATTCGACATGAGAACCTTGTGCCTCTTCTTGGTTATTGTAACGAAAAGGACCAAGAAATTCTTGTTTATCCATTTATGTCCAATGGCTCTCTGCAAGATCGCCTATATGGGGAAGCAGCAAAAAGAAAAATTCTGGACTGGCCAACAAGACTATCAATTGCTCTTGGTGCTGCTCGAGGTTTGACACATCTGCACAATTTTGCTGGGCGTTGCATTATACACAGGGATGTGAAATCAAGTAATATACTTCTGGATCAAAGCATGTGCGCCAAGGTGGCAGACTTTGGTTTTTCAAAATATGCTCCTCAAGAAGGTGATAGTGGTGCTTCTCTGGAAGTTAGAGGGACAGCTGGATACCTGGATCCAGAGTACTACACAACCCATCATTTATCAGCAAAAAGTGATGTGTTTAGCTTTGGCGTGGTTCTACTTGAAATTGTTACCGGTCGGGAACCTCTGAACATACACAGGCCACGTCCCGAGTGGAGCTTAGTCGAATGGGCAAAACCCTACATAAGAGAGTCAAAGATTGAAGAAATTGTTGATGCAAACATAAAAGGAGCATACCATGCAGAAGCTATGTGGAGGGTGGTGGAGGTAGCATTGTCATGTATTGAGCCCTTCTCTGCTTACCGGCCAGGCATGGCTGACATTGTTCGCGAGCTAGAGGATTCTCTGATAATAGAGAACAATGCATCAGAATACATGAGATCCATAGAAAGTACTGGATCTAATCGCTTTTCCATAATCATGGACAGAAGGATACCGCCACCTACTCCTTCGGAACCCTCAACTATTTTGACACAAATGGCCCCTCCAGAGCCAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0046777 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

937

Amino Acids

104.65

Weight (kDa)

5.44

Isoelectric Point (pI)

39.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	46 - 352	3.5e-35	Malectin-like domain
Malectin	PF11721	266 - 310	6.5e-06	Malectin domain
PK_Tyr_Ser-Thr	PF07714	613 - 882	4e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	613 - 815	1.6e-39	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013594)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g19010 FvH4_1g19010 FvH4_1g19010
malus_domestica	MD01G1044500.v1.1 MD15G1307000.v1.1
prunus_persica	Prupe.6G209400_v2.0.a1 Prupe.6G209400_v2.0.a1 Prupe.6G209400_v2.0.a1
pyrus_communis	pycom15g27100
rosa_chinensis	RchiOBHm_Chr2g0110021
rosa_laevigata	RLG00000017781
rosa_roxburghii	Rroxscaffold_2G00133770
rosa_rugosa	Rorug02G0165200
rosa_samantha	Rh2AG217000 Rh2BG226900 Rh2CG219100 Rh2DG222700
rosa_wichuraiana	Rw2G016760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1042
AasI	GACNNNNNNGTC	1 cut(s)	776
Acc36I	ACCTGC	1 cut(s)	1042
AccBSI	CCGCTC	1 cut(s)	1282
AccI	GTMKAC	1 cut(s)	666
AccII	CGCG	1 cut(s)	2640
AciI	CCGC	4 cut(s)	419, 772, 1282, 2747
AclI	AACGTT	1 cut(s)	705
AclWI	GGATC	8 cut(s)	1251, 1419, 1639, 2241, 2336, 2349, 2685, 2716
AcoI	YGGCCR	2 cut(s)	2120, 2615
AcuI	CTGAAG	4 cut(s)	108, 750, 770, 1513
AcyI	GRCGYC	2 cut(s)	714, 1004
AfaI	GTAC	7 cut(s)	620, 732, 833, 1393, 1811, 2351, 2704
AfiI	CCNNNNNNNGG	4 cut(s)	1193, 2009, 2089, 2291
AgeI	ACCGGT	1 cut(s)	2423
AhlI	ACTAGT	2 cut(s)	581, 1639
AjiI	CACGTC	1 cut(s)	2456
AjnI	CCWGG	4 cut(s)	669, 1187, 2337, 2617
AjuI	GAANNNNNNNTTGG	4 cut(s)	148, 180, 1845, 1877
Alw21I	GWGCWC	1 cut(s)	487
Alw26I	GTCTC	4 cut(s)	898, 1067, 1672, 1787
AlwI	GGATC	8 cut(s)	1251, 1419, 1639, 2241, 2336, 2349, 2685, 2716
AlwNI	CAGNNNCTG	1 cut(s)	645
Ama87I	CYCGRG	4 cut(s)	631, 1944, 2154, 2459
AoxI	GGCC	7 cut(s)	512, 793, 1436, 2120, 2450, 2615, 2790
ApeKI	GCWGC	9 cut(s)	83, 105, 126, 1491, 1616, 1619, 1622, 2096, 2150
AsiGI	ACCGGT	1 cut(s)	2423
AspLEI	GCGC	2 cut(s)	1059, 2249
AspS9I	GGNCC	7 cut(s)	269, 1022, 1295, 1436, 1866, 2029, 2791
AsuC2I	CCSGG	1 cut(s)	1657
AsuHPI	GGTGA	6 cut(s)	323, 481, 1120, 1319, 1816, 2306
AvaI	CYCGRG	4 cut(s)	631, 1944, 2154, 2459
AvaII	GGWCC	5 cut(s)	269, 1022, 1295, 1866, 2029
BalI	TGGCCA	1 cut(s)	2122
BamHI	GGATCC	1 cut(s)	2341
BanII	GRGCYC	1 cut(s)	2601
BbsI	GAAGAC	2 cut(s)	321, 1169
Bbv12I	GWGCWC	1 cut(s)	487
BbvCI	CCTCAGC	2 cut(s)	1346, 1500
BbvI	GCAGC	9 cut(s)	95, 113, 117, 1478, 1603, 1609, 1631, 2108, 2137
BccI	CCATC	5 cut(s)	307, 785, 1528, 1886, 2370
BceAI	ACGGC	1 cut(s)	923
BciT130I	CCWGG	4 cut(s)	671, 1189, 2339, 2619
BciVI	GTATCC	3 cut(s)	1731, 2327, 2736
BclI	TGATCA	1 cut(s)	1770
BcnI	CCSGG	1 cut(s)	1657
BcoDI	GTCTC	4 cut(s)	898, 1067, 1672, 1787
BcuI	ACTAGT	2 cut(s)	581, 1639
BfaI	CTAG	7 cut(s)	539, 582, 758, 1640, 1755, 1814, 2645
BfmI	CTRYAG	4 cut(s)	81, 1164, 1617, 1650
BfuAI	ACCTGC	1 cut(s)	1042
BfuI	GTATCC	3 cut(s)	1731, 2327, 2736
BglI	GCCNNNNNGGC	1 cut(s)	2255
BisI	GCNGC	9 cut(s)	84, 106, 127, 1492, 1617, 1620, 1623, 2097, 2151
BlsI	GCNGC	9 cut(s)	85, 107, 128, 1493, 1618, 1621, 1624, 2098, 2152
BmcAI	AGTACT	2 cut(s)	2351, 2704
Bme1390I	CCNGG	5 cut(s)	671, 1189, 1657, 2339, 2619
Bme18I	GGWCC	5 cut(s)	269, 1022, 1295, 1866, 2029
BmeT110I	CYCGRG	4 cut(s)	631, 1944, 2154, 2459
BmgBI	CACGTC	1 cut(s)	2456
BmgT120I	GGNCC	7 cut(s)	269, 1022, 1295, 1436, 1866, 2029, 2791
BmiI	GGNNCC	8 cut(s)	676, 1024, 1437, 1867, 2343, 2433, 2766, 2793
BmrFI	CCNGG	5 cut(s)	671, 1189, 1657, 2339, 2619
BmsI	GCATC	5 cut(s)	354, 1320, 1634, 2515, 2684
BoxI	GACNNNNGTC	2 cut(s)	1173, 1577
BpiI	GAAGAC	2 cut(s)	321, 1169
BplI	GAGNNNNNCTC	4 cut(s)	533, 565, 2588, 2620
BpmI	CTGGAG	2 cut(s)	1145, 2781
Bpu10I	CCTNAGC	2 cut(s)	1346, 1500
BpuEI	CTTGAG	4 cut(s)	1680, 1693, 1826, 2271
BpuMI	CCSGG	1 cut(s)	1657
BsaHI	GRCGYC	2 cut(s)	714, 1004
BsaJI	CCNNGG	7 cut(s)	444, 670, 1187, 1555, 1876, 1936, 2250
BsaWI	WCCGGW	1 cut(s)	2423
Bsc4I	CCNNNNNNNGG	4 cut(s)	1193, 2009, 2089, 2291
Bse118I	RCCGGY	2 cut(s)	2423, 2613
Bse1I	ACTGG	5 cut(s)	1060, 1245, 1579, 2123, 2710
Bse3DI	GCAATG	1 cut(s)	1039
BseBI	CCWGG	4 cut(s)	671, 1189, 2339, 2619
BseDI	CCNNGG	7 cut(s)	444, 670, 1187, 1555, 1876, 1936, 2250
BseGI	GGATG	4 cut(s)	404, 796, 1768, 2212
BseLI	CCNNNNNNNGG	4 cut(s)	1193, 2009, 2089, 2291
BseMI	GCAATG	1 cut(s)	1039
BseMII	CTCAG	6 cut(s)	144, 893, 999, 1360, 1406, 1514
BseNI	ACTGG	5 cut(s)	1060, 1245, 1579, 2123, 2710
BseRI	GAGGAG	3 cut(s)	1126, 1690, 2274
BseXI	GCAGC	9 cut(s)	95, 113, 117, 1478, 1603, 1609, 1631, 2108, 2137
BseYI	CCCAGC	2 cut(s)	863, 2183
BsgI	GTGCAG	1 cut(s)	2156
Bsh1236I	CGCG	1 cut(s)	2640
Bsh1285I	CGRYCG	1 cut(s)	2428
BshFI	GGCC	7 cut(s)	514, 795, 1438, 2122, 2452, 2617, 2792
BshTI	ACCGGT	1 cut(s)	2423
BsiEI	CGRYCG	1 cut(s)	2428
BsiHKAI	GWGCWC	1 cut(s)	487
BsiHKCI	CYCGRG	4 cut(s)	631, 1944, 2154, 2459
BsiSI	CCGG	3 cut(s)	1656, 2424, 2614
BslFI	GGGAC	3 cut(s)	1954, 2338, 2442
BslI	CCNNNNNNNGG	4 cut(s)	1193, 2009, 2089, 2291
BsmAI	GTCTC	4 cut(s)	898, 1067, 1672, 1787
BsmBI	CGTCTC	1 cut(s)	898
BsmFI	GGGAC	3 cut(s)	1954, 2338, 2442
BsmI	GAATGC	1 cut(s)	1257
BsnI	GGCC	7 cut(s)	514, 795, 1438, 2122, 2452, 2617, 2792
BsoBI	CYCGRG	4 cut(s)	631, 1944, 2154, 2459
Bsp1286I	GDGCHC	2 cut(s)	487, 2601
Bsp19I	CCATGG	1 cut(s)	1555
Bsp68I	TCGCGA	1 cut(s)	2640
BspACI	CCGC	4 cut(s)	419, 772, 1282, 2747
BspANI	GGCC	7 cut(s)	514, 795, 1438, 2122, 2452, 2617, 2792
BspCNI	CTCAG	6 cut(s)	143, 894, 1000, 1359, 1407, 1513
BspFNI	CGCG	1 cut(s)	2640
BspHI	TCATGA	1 cut(s)	1773
BspLI	GGNNCC	8 cut(s)	676, 1024, 1437, 1867, 2343, 2433, 2766, 2793
BspMAI	CTGCAG	3 cut(s)	85, 1168, 1621
BspMI	ACCTGC	1 cut(s)	1042
BspPI	GGATC	8 cut(s)	1251, 1419, 1639, 2241, 2336, 2349, 2685, 2716
BspQI	GCTCTTC	1 cut(s)	1272
BsrBI	CCGCTC	1 cut(s)	1282
BsrDI	GCAATG	1 cut(s)	1039
BsrFI	RCCGGY	2 cut(s)	2423, 2613
BsrI	ACTGG	5 cut(s)	1060, 1245, 1579, 2123, 2710
BssAI	RCCGGY	2 cut(s)	2423, 2613
BssECI	CCNNGG	7 cut(s)	444, 670, 1187, 1555, 1876, 1936, 2250
BssNI	GRCGYC	2 cut(s)	714, 1004
BssT1I	CCWWGG	4 cut(s)	444, 1555, 1936, 2250
Bst2UI	CCWGG	4 cut(s)	671, 1189, 2339, 2619
Bst4CI	ACNGT	8 cut(s)	367, 664, 856, 896, 1213, 1228, 1719, 1877
Bst6I	CTCTTC	5 cut(s)	62, 556, 863, 1272, 2010
BstACI	GRCGYC	2 cut(s)	714, 1004
BstAPI	GCANNNNNTGC	1 cut(s)	2549
BstC8I	GCNNGC	3 cut(s)	124, 954, 2642
BstDEI	CTNAG	9 cut(s)	130, 200, 902, 1008, 1317, 1346, 1415, 1500, 2470
BstDSI	CCRYGG	2 cut(s)	1555, 1876
BstENI	CCTNNNNNAGG	1 cut(s)	2289
BstF5I	GGATG	4 cut(s)	404, 796, 1768, 2212
BstFNI	CGCG	1 cut(s)	2640
BstHHI	GCGC	2 cut(s)	1059, 2249
BstMAI	GTCTC	4 cut(s)	898, 1067, 1672, 1787
BstMCI	CGRYCG	1 cut(s)	2428
BstMWI	GCNNNNNNNGC	5 cut(s)	907, 1622, 2147, 2255, 2549
BstNI	CCWGG	4 cut(s)	671, 1189, 2339, 2619
BstNSI	RCATGY	2 cut(s)	126, 2245
BstPAI	GACNNNNGTC	2 cut(s)	1173, 1577
BstSCI	CCNGG	5 cut(s)	669, 1187, 1655, 2337, 2617
BstSFI	CTRYAG	4 cut(s)	81, 1164, 1617, 1650
BstUI	CGCG	1 cut(s)	2640
BstV1I	GCAGC	9 cut(s)	95, 113, 117, 1478, 1603, 1609, 1631, 2108, 2137
BstV2I	GAAGAC	2 cut(s)	321, 1169
BstX2I	RGATCY	6 cut(s)	1243, 1411, 1631, 2341, 2690, 2708
BstXI	CCANNNNNNTGG	1 cut(s)	2731
BstYI	RGATCY	6 cut(s)	1243, 1411, 1631, 2341, 2690, 2708
BsuI	GTATCC	3 cut(s)	1731, 2327, 2736
BsuRI	GGCC	7 cut(s)	514, 795, 1438, 2122, 2452, 2617, 2792
BtgI	CCRYGG	2 cut(s)	1555, 1876
BtrI	CACGTC	1 cut(s)	2456
BtsCI	GGATG	4 cut(s)	404, 796, 1768, 2212
BtsI	GCAGTG	1 cut(s)	1914
BtsIMutI	CAGTG	5 cut(s)	901, 1224, 1413, 1537, 1914
BtuMI	TCGCGA	1 cut(s)	2640
BveI	ACCTGC	1 cut(s)	1042
Cac8I	GCNNGC	3 cut(s)	124, 954, 2642
CaiI	CAGNNNCTG	1 cut(s)	645
CciI	TCATGA	1 cut(s)	1773
CfoI	GCGC	2 cut(s)	1059, 2249
Cfr10I	RCCGGY	2 cut(s)	2423, 2613
Cfr13I	GGNCC	7 cut(s)	269, 1022, 1295, 1436, 1866, 2029, 2791
CseI	GACGC	2 cut(s)	703, 993
Csp6I	GTAC	7 cut(s)	619, 731, 832, 1392, 1810, 2350, 2703
CspAI	ACCGGT	1 cut(s)	2423
CviQI	GTAC	7 cut(s)	619, 731, 832, 1392, 1810, 2350, 2703
DdeI	CTNAG	9 cut(s)	130, 200, 902, 1008, 1317, 1346, 1415, 1500, 2470
DrdI	GACNNNNNNGTC	1 cut(s)	776
DseDI	GACNNNNNNGTC	1 cut(s)	776
EaeI	YGGCCR	2 cut(s)	2120, 2615
Eam1104I	CTCTTC	5 cut(s)	62, 556, 863, 1272, 2010
EarI	CTCTTC	5 cut(s)	62, 556, 863, 1272, 2010
Eco130I	CCWWGG	4 cut(s)	444, 1555, 1936, 2250
Eco147I	AGGCCT	1 cut(s)	514
Eco24I	GRGCYC	1 cut(s)	2601
Eco47I	GGWCC	5 cut(s)	269, 1022, 1295, 1866, 2029
Eco57I	CTGAAG	4 cut(s)	108, 750, 770, 1513
Eco88I	CYCGRG	4 cut(s)	631, 1944, 2154, 2459
EcoNI	CCTNNNNNAGG	1 cut(s)	2289
EcoRII	CCWGG	4 cut(s)	669, 1187, 2337, 2617
EcoT14I	CCWWGG	4 cut(s)	444, 1555, 1936, 2250
EcoT22I	ATGCAT	1 cut(s)	2677
EcoT38I	GRGCYC	1 cut(s)	2601
ErhI	CCWWGG	4 cut(s)	444, 1555, 1936, 2250
Esp3I	CGTCTC	1 cut(s)	898
FalI	AAGNNNNNCTT	2 cut(s)	2025, 2057
FaqI	GGGAC	3 cut(s)	1954, 2338, 2442
FbaI	TGATCA	1 cut(s)	1770
FblI	GTMKAC	1 cut(s)	666
Fnu4HI	GCNGC	9 cut(s)	84, 106, 127, 1492, 1617, 1620, 1623, 2097, 2151
FokI	GGATG	4 cut(s)	391, 803, 1775, 2219
FriOI	GRGCYC	1 cut(s)	2601
Fsp4HI	GCNGC	9 cut(s)	84, 106, 127, 1492, 1617, 1620, 1623, 2097, 2151
FspBI	CTAG	7 cut(s)	539, 582, 758, 1640, 1755, 1814, 2645
GlaI	GCGC	2 cut(s)	1058, 2248
GluI	GCNGC	9 cut(s)	84, 106, 127, 1492, 1617, 1620, 1623, 2097, 2151
GsaI	CCCAGC	2 cut(s)	867, 2187
GsuI	CTGGAG	2 cut(s)	1145, 2781
HaeIII	GGCC	7 cut(s)	514, 795, 1438, 2122, 2452, 2617, 2792
HapII	CCGG	3 cut(s)	1656, 2424, 2614
HgaI	GACGC	2 cut(s)	703, 993
HhaI	GCGC	2 cut(s)	1059, 2249
Hin1I	GRCGYC	2 cut(s)	714, 1004
Hin6I	GCGC	2 cut(s)	1057, 2247
HinP1I	GCGC	2 cut(s)	1057, 2247
HincII	GTYRAC	5 cut(s)	241, 651, 667, 703, 1977
HindII	GTYRAC	5 cut(s)	241, 651, 667, 703, 1977
HindIII	AAGCTT	1 cut(s)	1685
HpaI	GTTAAC	2 cut(s)	241, 651
HpaII	CCGG	3 cut(s)	1656, 2424, 2614
HphI	GGTGA	6 cut(s)	323, 481, 1120, 1319, 1816, 2306
Hpy166II	GTNNAC	9 cut(s)	241, 651, 667, 703, 886, 1161, 1362, 1873, 1977
Hpy8I	GTNNAC	9 cut(s)	241, 651, 667, 703, 886, 1161, 1362, 1873, 1977
HpyCH4III	ACNGT	8 cut(s)	367, 664, 856, 896, 1213, 1228, 1719, 1877
HpyCH4IV	ACGT	4 cut(s)	264, 705, 1390, 2455
HpyF10VI	GCNNNNNNNGC	5 cut(s)	907, 1622, 2147, 2255, 2549
HpyF3I	CTNAG	9 cut(s)	130, 200, 902, 1008, 1317, 1346, 1415, 1500, 2470
HpySE526I	ACGT	4 cut(s)	264, 705, 1390, 2455
Hsp92I	GRCGYC	2 cut(s)	714, 1004
HspAI	GCGC	2 cut(s)	1057, 2247
Ksp22I	TGATCA	1 cut(s)	1770
KspAI	GTTAAC	2 cut(s)	241, 651
LguI	GCTCTTC	1 cut(s)	1272
LmnI	GCTCC	5 cut(s)	482, 949, 2287, 2466, 2540
Lsp1109I	GCAGC	9 cut(s)	95, 113, 117, 1478, 1603, 1609, 1631, 2108, 2137
LweI	GCATC	5 cut(s)	354, 1320, 1634, 2515, 2684
MaeI	CTAG	7 cut(s)	539, 582, 758, 1640, 1755, 1814, 2645
MaeII	ACGT	4 cut(s)	264, 705, 1390, 2455
MbiI	CCGCTC	1 cut(s)	1282
MfeI	CAATTG	2 cut(s)	222, 2136
MflI	RGATCY	6 cut(s)	1243, 1411, 1631, 2341, 2690, 2708
MhlI	GDGCHC	2 cut(s)	487, 2601
MlsI	TGGCCA	1 cut(s)	2122
MluNI	TGGCCA	1 cut(s)	2122
MlyI	GAGTC	6 cut(s)	438, 985, 1019, 1318, 1936, 2510
Mox20I	TGGCCA	1 cut(s)	2122
Mph1103I	ATGCAT	1 cut(s)	2677
MscI	TGGCCA	1 cut(s)	2122
MslI	CAYNNNNRTG	4 cut(s)	377, 2205, 2587, 2729
Msp20I	TGGCCA	1 cut(s)	2122
MspA1I	CMGCKG	4 cut(s)	86, 1622, 1649, 2330
MspI	CCGG	3 cut(s)	1656, 2424, 2614
MspR9I	CCNGG	5 cut(s)	671, 1189, 1657, 2339, 2619
MunI	CAATTG	2 cut(s)	222, 2136
Mva1269I	GAATGC	1 cut(s)	1257
MvaI	CCWGG	4 cut(s)	671, 1189, 2339, 2619
MvnI	CGCG	1 cut(s)	2640
MwoI	GCNNNNNNNGC	5 cut(s)	907, 1622, 2147, 2255, 2549
NciI	CCSGG	1 cut(s)	1657
NcoI	CCATGG	1 cut(s)	1555
NlaIV	GGNNCC	8 cut(s)	676, 1024, 1437, 1867, 2343, 2433, 2766, 2793
NmuCI	GTSAC	8 cut(s)	379, 473, 635, 715, 964, 1222, 1570, 1822
NruI	TCGCGA	1 cut(s)	2640
NsiI	ATGCAT	1 cut(s)	2677
NspI	RCATGY	2 cut(s)	126, 2245
PaeI	GCATGC	1 cut(s)	126
PaeR7I	CTCGAG	1 cut(s)	2154
PagI	TCATGA	1 cut(s)	1773
PaqCI	CACCTGC	1 cut(s)	1042
PceI	AGGCCT	1 cut(s)	514
PciSI	GCTCTTC	1 cut(s)	1272
PcsI	WCGNNNNNNNCGW	1 cut(s)	711
PctI	GAATGC	1 cut(s)	1257
PfeI	GAWTC	6 cut(s)	134, 502, 642, 985, 1602, 2651
PinAI	ACCGGT	1 cut(s)	2423
PkrI	GCNGC	9 cut(s)	85, 107, 128, 1493, 1618, 1621, 1624, 2098, 2152
PleI	GAGTC	6 cut(s)	437, 985, 1018, 1318, 1936, 2509
PpsI	GAGTC	6 cut(s)	437, 985, 1018, 1318, 1936, 2509
PshAI	GACNNNNGTC	2 cut(s)	1173, 1577
Psp1406I	AACGTT	1 cut(s)	705
Psp6I	CCWGG	4 cut(s)	669, 1187, 2337, 2617
PspFI	CCCAGC	2 cut(s)	863, 2183
PspGI	CCWGG	4 cut(s)	669, 1187, 2337, 2617
PspN4I	GGNNCC	8 cut(s)	676, 1024, 1437, 1867, 2343, 2433, 2766, 2793
PspPI	GGNCC	7 cut(s)	269, 1022, 1295, 1436, 1866, 2029, 2791
PspXI	VCTCGAGB	1 cut(s)	2154
PsrI	GAACNNNNNNTAC	2 cut(s)	515, 547
PstI	CTGCAG	3 cut(s)	85, 1168, 1621
PstNI	CAGNNNCTG	1 cut(s)	645
PsuI	RGATCY	6 cut(s)	1243, 1411, 1631, 2341, 2690, 2708
PvuII	CAGCTG	4 cut(s)	86, 1622, 1649, 2330
RruI	TCGCGA	1 cut(s)	2640
RsaI	GTAC	7 cut(s)	620, 732, 833, 1393, 1811, 2351, 2704
RsaNI	GTAC	7 cut(s)	619, 731, 832, 1392, 1810, 2350, 2703
RseI	CAYNNNNRTG	4 cut(s)	377, 2205, 2587, 2729
SalI	GTCGAC	1 cut(s)	665
SapI	GCTCTTC	1 cut(s)	1272
SatI	GCNGC	9 cut(s)	84, 106, 127, 1492, 1617, 1620, 1623, 2097, 2151
Sau96I	GGNCC	7 cut(s)	269, 1022, 1295, 1436, 1866, 2029, 2791
ScaI	AGTACT	2 cut(s)	2351, 2704
SchI	GAGTC	6 cut(s)	438, 985, 1019, 1318, 1936, 2510
ScrFI	CCNGG	5 cut(s)	671, 1189, 1657, 2339, 2619
SduI	GDGCHC	2 cut(s)	487, 2601
SfaNI	GCATC	5 cut(s)	354, 1320, 1634, 2515, 2684
SfcI	CTRYAG	4 cut(s)	81, 1164, 1617, 1650
Sfr274I	CTCGAG	1 cut(s)	2154
SinI	GGWCC	5 cut(s)	269, 1022, 1295, 1866, 2029
SlaI	CTCGAG	1 cut(s)	2154
SmiMI	CAYNNNNRTG	4 cut(s)	377, 2205, 2587, 2729
SmlI	CTYRAG	5 cut(s)	1672, 1695, 1805, 2154, 2286
SmoI	CTYRAG	5 cut(s)	1672, 1695, 1805, 2154, 2286
SpeI	ACTAGT	2 cut(s)	581, 1639
SphI	GCATGC	1 cut(s)	126
SseBI	AGGCCT	1 cut(s)	514
SsiI	CCGC	4 cut(s)	419, 772, 1282, 2747
SspI	AATATT	1 cut(s)	1743
SspMI	CTAG	7 cut(s)	539, 582, 758, 1640, 1755, 1814, 2645
StuI	AGGCCT	1 cut(s)	514
StyD4I	CCNGG	5 cut(s)	669, 1187, 1655, 2337, 2617
StyI	CCWWGG	4 cut(s)	444, 1555, 1936, 2250
TaaI	ACNGT	8 cut(s)	367, 664, 856, 896, 1213, 1228, 1719, 1877
TaiI	ACGT	4 cut(s)	267, 708, 1393, 2458
TaqI	TCGA	7 cut(s)	666, 727, 1592, 1918, 1984, 2155, 2475
TatI	WGTACW	3 cut(s)	831, 2349, 2702
TfiI	GAWTC	6 cut(s)	134, 502, 642, 985, 1602, 2651
TscAI	CASTG	5 cut(s)	901, 1231, 1413, 1537, 1914
TseFI	GTSAC	8 cut(s)	379, 473, 635, 715, 964, 1222, 1570, 1822
TseI	GCWGC	9 cut(s)	83, 105, 126, 1491, 1616, 1619, 1622, 2096, 2150
Tsp45I	GTSAC	8 cut(s)	379, 473, 635, 715, 964, 1222, 1570, 1822
TspDTI	ATGAA	9 cut(s)	17, 34, 49, 601, 855, 1154, 1158, 1290, 1790
TspGWI	ACGGA	1 cut(s)	1858
TspRI	CASTG	5 cut(s)	901, 1231, 1413, 1537, 1914
VpaK11BI	GGWCC	5 cut(s)	269, 1022, 1295, 1866, 2029
XagI	CCTNNNNNAGG	1 cut(s)	2289
XbaI	TCTAGA	1 cut(s)	538
XceI	RCATGY	2 cut(s)	126, 2245
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1562, 2460
XhoI	CTCGAG	1 cut(s)	2154
XmiI	GTMKAC	1 cut(s)	666
XspI	CTAG	7 cut(s)	539, 582, 758, 1640, 1755, 1814, 2645
ZrmI	AGTACT	2 cut(s)	2351, 2704
Zsp2I	ATGCAT	1 cut(s)	2677

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.