Rh2CG219100

Nodulation receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0013594
Physical Location & Seq
Forward (+)
21647545 .. 21656490
8946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG219100.1

Sequence Viewer

Length: 2817 bp
ATGATGAAGCAATTAAACATTTGGGTTTTAAGACTGACAGAGTTCTTCATACTTTGCCTCTTCATTTTTAATCCATTGACTTCTGCAGATGAAGGCAGTAATTCAAGCAGCTTTGTGAGTATTGCATGCTGCTCAGAATCTAATTTTACAGATATAAGAAACATAAGTTGGGTAACAGACTTTAATGAGTTTCGTAATAGGCCAAGTTGTGAGGACAGAAAGCCAATTGCGATTGATTATCTTAACAATAAAACAAGGGTGTTTCACGTTGGTCCAAATCAGAAAAGATGTTATACCTTACCGTCAAAAAAGGGTGAAGACTATCTAATAAGGGGTATTTTCGGAGGCATCTGGGGAGTGAAATTTACAGTTGCCGTTGGTGTGACCACAATCGGAGTAGTAGATACATCCGATGATATAGCGGTTGAGGGAGTCTTTAGAGCTACCAAGGACTATACACACTTTTGCTTGGTAAGTGACGAAGGAGCACTTTATATTTCAGAGATTCAGTTGAGGCCTCTGTGGAACTTGGGTTACTATCTAGAGGACTTCTCTTCAAGTGTTCTGGAACTGCTTAAAAGGACTAGTTTTGATGATATAAGAGCAGCAAAGTTCATCAGGTACGAAGCTGACCCGAGTGACAGAATCTGGTTAACAAACTCAACTGTCGACCTGGGAACCTTCCACATAACAGAAGAACCCAATGTCAACTTTCTGGGCGTCACAGATGCTCGAGTACCCATTCAAGTGCTCCAAACAGCTCTAGCTTCTTCAGACCGCTTGGTCATTCTTCACGATGGCCTGTCCTCAACAAACAATCACTACCTTTTGTTCCTGTACTTCTATGAAGTCAATCACACTGTTCAAGCTGGGAAGAGGGTTTTTGATGTTTACTTGAACAGTGAGCTGAGACGGCAAGGATTTGATGTTATGGGTAATGGGTCAAATTACAGGGAGCTTGCTTTGAATGTGACAGCAAATGGGTATCTGAATCTGACTCTGGTTAAGGCGTTTGAGTCTGAAAATGGACCCATTTGCAATGCCTATGAGATTTTGCAGGTGCACCAGTTGGTTCAGGAGACTAACACAGATGACGTGGAAGTGATTTTGGAGGCGAGGAGTGAGTTGCTGGAGAATAATCAGATGAATGAAGTTTGGGAAGGGTGGACTGCAGACCCATGTCTTCCTGTTCCCTGGAATGGTATAGGTTGTGAAACTGTTAATGGTGTCACTGTCATTACCAAACTGGATCTTTCTTCTGGTATTCAGAGAGGGTCTGATGAAGAACGCAAACTTCTAGGACCATTTCCTAAAAGCATCACAAAGCTGACTCACCTAAAATTCCTGAACCTCAGCAACAATGGGTTTACTGGCCAAATTCCAGAATTTCTTGAACGTTCCATGTTGATTTCAGTGGATCTGAGCCATAATAATCTTTCGGGGCCACTTCCAAATTCTCTTGCCTCACAACCAAATTTGGAAACACTAAATTTTGGCTGCAATCCTCAGCTTACCAAAGTTAGTCCATCAACCTTCAATGGCTTAAAATTTACAACAGACCATGGAAATTGTGGTGTGACTGGTGTCCAAAAATTCTCGAAACGAGGAATCATCATTGGTGCTGCTGCTGCATCTGGATCTTTACTAGTAACAGCTGTAGCCGGGATTTTTTTTTTCATGTGGAGACAAAAGTTTATGCCTCGAGGAAAATTTGATAAGAACCCGTATCCCATGGCAAAGAATTTAATTTTCTCATTACCTAGCATGGATGATCTGATGATGAAATCAATAGCAATAGAGACATTTACTCTTGAGTACCTAGAGGCTGTCACCCAAAGTTACAAAACGCTGATAGGTGAAGGAGGTTTTGGGTCTGTTTACAGTGGCACTCTAACTGATGGTCAGGAAGTGGCAGTGAAGGTTCGATCAGCAACATCAACCCAAGGGACTCGGGAGTTTGATAATGAGCTAAATCTTCTGTCAACTATTCGGCATGAGAACCTTGTGCCTCTTCTTGGTTATTGTAATGAAAAGGACCAAGAAATTCTTGTTTATCCATTTATGTCCAATGGCTCTCTGCAAGATCGCCTATATGGGGAAGCAGCAAAAAGGAAAATTCTGGACTGGCCAACAAGACTATCAATTGCCCTTGGTGCTGCTCGAGGTTTGACACATCTGCACAATTTTGCTGGGCGTTGCATTATACACAGGGATGTGAAATCAAGTAATATACTTCTGGATCAAAGCATGTGTGCCAAGGTGGCAGACTTTGGTTTTTCAAAATATGCTCCTCAAGAAGGTGACAGTGGTGCTTCTCTGGAAGTTAGAGGGACAGCTGGATACCTGGATCCAGAGTACTACACAACCCATCATTTATCAGCAAAAAGTGATGTGTTTAGCTTTGGCGTGGTTCTACTTGAAATTGTTACTGGTCGGGAACCTCTGAACATACACAGGCCACGTCCCGAGTGGAGCTTAGTCGAATGGGCGAAACCCTATATAAGAGAGTCAAAGATTGATGAAATTGTGGATCCAAACATAAAAGGAGCATACCATGCAGAAGCTATGTGGAGGGTGGTGGAGGTAGCATTGTCATGTATTGAGCCCTTCTCTGCTTACCGACCAGGCATGGCTGACATTGTTCGCGAGCTAGAGGATTCTCTGATAATAGAGAACAATGCATCAGAATACATGAGATCCATAGAAAGTACTGGATCTAATCGCTTTTCCATAATCATGGACAGAAGGATACCGCCACCTACTCCTTCGGAACCCTCAACTATTTTGACACAAATGGCCCCTCCAGAGCCAAGATAG

Protein Analysis

938

Amino Acids

104.91

Weight (kDa)

5.35

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 49 - 354 9.4e-33 Malectin-like domain
Malectin PF11721 268 - 312 2.1e-06 Malectin domain
PK_Tyr_Ser-Thr PF07714 615 - 884 3.1e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 615 - 817 2.3e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1048
AasI GACNNNNNNGTC 1 cut(s) 782
AbsI CCTCGAGG 1 cut(s) 1701
Acc36I ACCTGC 1 cut(s) 1048
AccI GTMKAC 1 cut(s) 669
AccII CGCG 1 cut(s) 2646
AciI CCGC 3 cut(s) 422, 778, 2753
AclI AACGTT 1 cut(s) 1396
AcoI YGGCCR 2 cut(s) 1372, 2126
AcuI CTGAAG 1 cut(s) 756
AcyI GRCGYC 1 cut(s) 720
AfaI GTAC 6 cut(s) 623, 738, 839, 1817, 2357, 2710
AfiI CCNNNNNNNGG 4 cut(s) 1199, 2015, 2095, 2297
AhlI ACTAGT 2 cut(s) 584, 1645
AjiI CACGTC 2 cut(s) 1096, 2462
AjnI CCWGG 4 cut(s) 672, 1193, 2343, 2623
AjuI GAANNNNNNNTTGG 4 cut(s) 151, 183, 1851, 1883
Alw21I GWGCWC 3 cut(s) 490, 753, 1065
Alw26I GTCTC 4 cut(s) 904, 1073, 1678, 1793
Alw44I GTGCAC 1 cut(s) 1061
AlwNI CAGNNNCTG 1 cut(s) 648
Ama87I CYCGRG 6 cut(s) 634, 732, 1701, 1950, 2160, 2465
AoxI GGCC 8 cut(s) 200, 515, 799, 1372, 1442, 2126, 2456, 2796
ApaLI GTGCAC 1 cut(s) 1061
ApeKI GCWGC 9 cut(s) 108, 129, 605, 1497, 1622, 1625, 1628, 2102, 2156
AspS9I GGNCC 6 cut(s) 272, 1028, 1301, 1442, 2035, 2797
AsuC2I CCSGG 1 cut(s) 1663
AsuHPI GGTGA 5 cut(s) 326, 1325, 1822, 1868, 2312
AvaI CYCGRG 6 cut(s) 634, 732, 1701, 1950, 2160, 2465
AvaII GGWCC 4 cut(s) 272, 1028, 1301, 2035
BaeGI GKGCMC 1 cut(s) 1065
BalI TGGCCA 2 cut(s) 1374, 2128
BamHI GGATCC 2 cut(s) 2347, 2530
BanII GRGCYC 1 cut(s) 2607
BbsI GAAGAC 2 cut(s) 324, 1175
Bbv12I GWGCWC 3 cut(s) 490, 753, 1065
BbvCI CCTCAGC 2 cut(s) 1352, 1506
BbvI GCAGC 9 cut(s) 116, 120, 617, 1484, 1609, 1612, 1615, 2114, 2143
BccI CCATC 4 cut(s) 791, 1534, 1892, 2376
BceAI ACGGC 2 cut(s) 359, 929
BciT130I CCWGG 4 cut(s) 674, 1195, 2345, 2625
BciVI GTATCC 3 cut(s) 1737, 2333, 2742
BcnI CCSGG 1 cut(s) 1663
BcoDI GTCTC 4 cut(s) 904, 1073, 1678, 1793
BcuI ACTAGT 2 cut(s) 584, 1645
BfaI CTAG 8 cut(s) 542, 585, 764, 1298, 1646, 1761, 1820, 2651
BfmI CTRYAG 3 cut(s) 84, 1170, 1656
BfuAI ACCTGC 1 cut(s) 1048
BfuI GTATCC 3 cut(s) 1737, 2333, 2742
BglI GCCNNNNNGGC 1 cut(s) 2261
BisI GCNGC 9 cut(s) 109, 130, 606, 1498, 1623, 1626, 1629, 2103, 2157
BlsI GCNGC 9 cut(s) 110, 131, 607, 1499, 1624, 1627, 1630, 2104, 2158
BmcAI AGTACT 2 cut(s) 2357, 2710
Bme1390I CCNGG 5 cut(s) 674, 1195, 1663, 2345, 2625
Bme18I GGWCC 4 cut(s) 272, 1028, 1301, 2035
BmeT110I CYCGRG 6 cut(s) 634, 732, 1701, 1950, 2160, 2465
BmgBI CACGTC 2 cut(s) 1096, 2462
BmgT120I GGNCC 6 cut(s) 272, 1028, 1301, 1442, 2035, 2797
BmiI GGNNCC 8 cut(s) 679, 1030, 1443, 2349, 2439, 2532, 2772, 2799
BmrFI CCNGG 5 cut(s) 674, 1195, 1663, 2345, 2625
BmsI GCATC 5 cut(s) 357, 718, 1326, 1640, 2690
BoxI GACNNNNGTC 2 cut(s) 1179, 1583
BpiI GAAGAC 2 cut(s) 324, 1175
BplI GAGNNNNNCTC 4 cut(s) 536, 568, 2594, 2626
BpmI CTGGAG 2 cut(s) 1151, 2787
Bpu10I CCTNAGC 2 cut(s) 1352, 1506
BpuEI CTTGAG 2 cut(s) 1832, 2277
BpuMI CCSGG 1 cut(s) 1663
BsaHI GRCGYC 1 cut(s) 720
BsaJI CCNNGG 8 cut(s) 447, 673, 1193, 1561, 1731, 1942, 2149, 2256
Bsc4I CCNNNNNNNGG 4 cut(s) 1199, 2015, 2095, 2297
Bse1I ACTGG 7 cut(s) 1066, 1251, 1375, 1585, 2129, 2434, 2716
Bse3DI GCAATG 1 cut(s) 1045
BseBI CCWGG 4 cut(s) 674, 1195, 2345, 2625
BseDI CCNNGG 8 cut(s) 447, 673, 1193, 1561, 1731, 1942, 2149, 2256
BseGI GGATG 3 cut(s) 407, 1774, 2218
BseLI CCNNNNNNNGG 4 cut(s) 1199, 2015, 2095, 2297
BseMI GCAATG 1 cut(s) 1045
BseMII CTCAG 5 cut(s) 147, 899, 1366, 1412, 1520
BseNI ACTGG 7 cut(s) 1066, 1251, 1375, 1585, 2129, 2434, 2716
BseRI GAGGAG 2 cut(s) 1132, 2280
BseSI GKGCMC 1 cut(s) 1065
BseXI GCAGC 9 cut(s) 116, 120, 617, 1484, 1609, 1612, 1615, 2114, 2143
BseYI CCCAGC 2 cut(s) 869, 2189
BsgI GTGCAG 1 cut(s) 2162
Bsh1236I CGCG 1 cut(s) 2646
BshFI GGCC 8 cut(s) 202, 517, 801, 1374, 1444, 2128, 2458, 2798
BsiHKAI GWGCWC 3 cut(s) 490, 753, 1065
BsiHKCI CYCGRG 6 cut(s) 634, 732, 1701, 1950, 2160, 2465
BsiSI CCGG 1 cut(s) 1662
BslFI GGGAC 3 cut(s) 1960, 2344, 2448
BslI CCNNNNNNNGG 4 cut(s) 1199, 2015, 2095, 2297
BsmAI GTCTC 4 cut(s) 904, 1073, 1678, 1793
BsmBI CGTCTC 1 cut(s) 904
BsmFI GGGAC 3 cut(s) 1960, 2344, 2448
BsnI GGCC 8 cut(s) 202, 517, 801, 1374, 1444, 2128, 2458, 2798
BsoBI CYCGRG 6 cut(s) 634, 732, 1701, 1950, 2160, 2465
Bsp1286I GDGCHC 4 cut(s) 490, 753, 1065, 2607
Bsp19I CCATGG 2 cut(s) 1561, 1731
Bsp68I TCGCGA 1 cut(s) 2646
BspACI CCGC 3 cut(s) 422, 778, 2753
BspANI GGCC 8 cut(s) 202, 517, 801, 1374, 1444, 2128, 2458, 2798
BspCNI CTCAG 5 cut(s) 146, 900, 1365, 1413, 1519
BspFNI CGCG 1 cut(s) 2646
BspLI GGNNCC 8 cut(s) 679, 1030, 1443, 2349, 2439, 2532, 2772, 2799
BspMAI CTGCAG 2 cut(s) 88, 1174
BspMI ACCTGC 1 cut(s) 1048
BsrDI GCAATG 1 cut(s) 1045
BsrI ACTGG 7 cut(s) 1066, 1251, 1375, 1585, 2129, 2434, 2716
BssECI CCNNGG 8 cut(s) 447, 673, 1193, 1561, 1731, 1942, 2149, 2256
BssNI GRCGYC 1 cut(s) 720
BssT1I CCWWGG 6 cut(s) 447, 1561, 1731, 1942, 2149, 2256
Bst2UI CCWGG 4 cut(s) 674, 1195, 2345, 2625
Bst4CI ACNGT 9 cut(s) 303, 370, 667, 862, 902, 1219, 1234, 1883, 2306
Bst6I CTCTTC 4 cut(s) 65, 559, 869, 2016
BstACI GRCGYC 1 cut(s) 720
BstAPI GCANNNNNTGC 1 cut(s) 2555
BstC8I GCNNGC 3 cut(s) 127, 960, 2648
BstDEI CTNAG 6 cut(s) 133, 908, 1352, 1421, 1506, 2476
BstDSI CCRYGG 2 cut(s) 1561, 1731
BstENI CCTNNNNNAGG 1 cut(s) 2295
BstF5I GGATG 3 cut(s) 407, 1774, 2218
BstFNI CGCG 1 cut(s) 2646
BstMAI GTCTC 4 cut(s) 904, 1073, 1678, 1793
BstMWI GCNNNNNNNGC 5 cut(s) 913, 1628, 2153, 2261, 2555
BstNI CCWGG 4 cut(s) 674, 1195, 2345, 2625
BstNSI RCATGY 2 cut(s) 129, 2251
BstPAI GACNNNNGTC 2 cut(s) 1179, 1583
BstSCI CCNGG 5 cut(s) 672, 1193, 1661, 2343, 2623
BstSFI CTRYAG 3 cut(s) 84, 1170, 1656
BstSLI GKGCMC 1 cut(s) 1065
BstUI CGCG 1 cut(s) 2646
BstV1I GCAGC 9 cut(s) 116, 120, 617, 1484, 1609, 1612, 1615, 2114, 2143
BstV2I GAAGAC 2 cut(s) 324, 1175
BstX2I RGATCY 7 cut(s) 1249, 1417, 1637, 2347, 2530, 2696, 2714
BstXI CCANNNNNNTGG 1 cut(s) 2737
BstYI RGATCY 7 cut(s) 1249, 1417, 1637, 2347, 2530, 2696, 2714
BsuI GTATCC 3 cut(s) 1737, 2333, 2742
BsuRI GGCC 8 cut(s) 202, 517, 801, 1374, 1444, 2128, 2458, 2798
BtgI CCRYGG 2 cut(s) 1561, 1731
BtrI CACGTC 2 cut(s) 1096, 2462
BtsCI GGATG 3 cut(s) 407, 1774, 2218
BtsI GCAGTG 1 cut(s) 1920
BtsIMutI CAGTG 7 cut(s) 858, 907, 1230, 1419, 1888, 1920, 2311
BtuMI TCGCGA 1 cut(s) 2646
BveI ACCTGC 1 cut(s) 1048
Cac8I GCNNGC 3 cut(s) 127, 960, 2648
CaiI CAGNNNCTG 1 cut(s) 648
Cfr13I GGNCC 6 cut(s) 272, 1028, 1301, 1442, 2035, 2797
CseI GACGC 1 cut(s) 709
Csp6I GTAC 6 cut(s) 622, 737, 838, 1816, 2356, 2709
CviQI GTAC 6 cut(s) 622, 737, 838, 1816, 2356, 2709
DdeI CTNAG 6 cut(s) 133, 908, 1352, 1421, 1506, 2476
DrdI GACNNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 782
EaeI YGGCCR 2 cut(s) 1372, 2126
Eam1104I CTCTTC 4 cut(s) 65, 559, 869, 2016
EarI CTCTTC 4 cut(s) 65, 559, 869, 2016
Eco130I CCWWGG 6 cut(s) 447, 1561, 1731, 1942, 2149, 2256
Eco147I AGGCCT 1 cut(s) 517
Eco24I GRGCYC 1 cut(s) 2607
Eco47I GGWCC 4 cut(s) 272, 1028, 1301, 2035
Eco57I CTGAAG 1 cut(s) 756
Eco88I CYCGRG 6 cut(s) 634, 732, 1701, 1950, 2160, 2465
EcoNI CCTNNNNNAGG 1 cut(s) 2295
EcoRII CCWGG 4 cut(s) 672, 1193, 2343, 2623
EcoT14I CCWWGG 6 cut(s) 447, 1561, 1731, 1942, 2149, 2256
EcoT22I ATGCAT 1 cut(s) 2683
EcoT38I GRGCYC 1 cut(s) 2607
ErhI CCWWGG 6 cut(s) 447, 1561, 1731, 1942, 2149, 2256
Esp3I CGTCTC 1 cut(s) 904
FalI AAGNNNNNCTT 4 cut(s) 474, 506, 2031, 2063
FaqI GGGAC 3 cut(s) 1960, 2344, 2448
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 9 cut(s) 109, 130, 606, 1498, 1623, 1626, 1629, 2103, 2157
FokI GGATG 3 cut(s) 394, 1781, 2225
FriOI GRGCYC 1 cut(s) 2607
Fsp4HI GCNGC 9 cut(s) 109, 130, 606, 1498, 1623, 1626, 1629, 2103, 2157
FspBI CTAG 8 cut(s) 542, 585, 764, 1298, 1646, 1761, 1820, 2651
GluI GCNGC 9 cut(s) 109, 130, 606, 1498, 1623, 1626, 1629, 2103, 2157
GsaI CCCAGC 2 cut(s) 873, 2193
GsuI CTGGAG 2 cut(s) 1151, 2787
HaeIII GGCC 8 cut(s) 202, 517, 801, 1374, 1444, 2128, 2458, 2798
HapII CCGG 1 cut(s) 1662
HgaI GACGC 1 cut(s) 709
Hin1I GRCGYC 1 cut(s) 720
HincII GTYRAC 4 cut(s) 654, 670, 709, 1983
HindII GTYRAC 4 cut(s) 654, 670, 709, 1983
HpaI GTTAAC 1 cut(s) 654
HpaII CCGG 1 cut(s) 1662
HphI GGTGA 5 cut(s) 326, 1325, 1822, 1868, 2312
Hpy166II GTNNAC 9 cut(s) 654, 670, 709, 892, 1063, 1167, 1368, 1879, 1983
Hpy8I GTNNAC 9 cut(s) 654, 670, 709, 892, 1063, 1167, 1368, 1879, 1983
HpyCH4III ACNGT 9 cut(s) 303, 370, 667, 862, 902, 1219, 1234, 1883, 2306
HpyCH4IV ACGT 4 cut(s) 267, 1095, 1396, 2461
HpyF10VI GCNNNNNNNGC 5 cut(s) 913, 1628, 2153, 2261, 2555
HpyF3I CTNAG 6 cut(s) 133, 908, 1352, 1421, 1506, 2476
HpySE526I ACGT 4 cut(s) 267, 1095, 1396, 2461
Hsp92I GRCGYC 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 654
LmnI GCTCC 6 cut(s) 485, 756, 955, 2293, 2472, 2546
Lsp1109I GCAGC 9 cut(s) 116, 120, 617, 1484, 1609, 1612, 1615, 2114, 2143
LweI GCATC 5 cut(s) 357, 718, 1326, 1640, 2690
MaeI CTAG 8 cut(s) 542, 585, 764, 1298, 1646, 1761, 1820, 2651
MaeII ACGT 4 cut(s) 267, 1095, 1396, 2461
MfeI CAATTG 2 cut(s) 225, 2142
MflI RGATCY 7 cut(s) 1249, 1417, 1637, 2347, 2530, 2696, 2714
MhlI GDGCHC 4 cut(s) 490, 753, 1065, 2607
MlsI TGGCCA 2 cut(s) 1374, 2128
MluNI TGGCCA 2 cut(s) 1374, 2128
MlyI GAGTC 6 cut(s) 441, 991, 1025, 1324, 1942, 2516
Mox20I TGGCCA 2 cut(s) 1374, 2128
Mph1103I ATGCAT 1 cut(s) 2683
MscI TGGCCA 2 cut(s) 1374, 2128
MslI CAYNNNNRTG 4 cut(s) 746, 2211, 2593, 2735
Msp20I TGGCCA 2 cut(s) 1374, 2128
MspA1I CMGCKG 2 cut(s) 1655, 2336
MspI CCGG 1 cut(s) 1662
MspR9I CCNGG 5 cut(s) 674, 1195, 1663, 2345, 2625
MunI CAATTG 2 cut(s) 225, 2142
MvaI CCWGG 4 cut(s) 674, 1195, 2345, 2625
MvnI CGCG 1 cut(s) 2646
MwoI GCNNNNNNNGC 5 cut(s) 913, 1628, 2153, 2261, 2555
NciI CCSGG 1 cut(s) 1663
NcoI CCATGG 2 cut(s) 1561, 1731
NlaIV GGNNCC 8 cut(s) 679, 1030, 1443, 2349, 2439, 2532, 2772, 2799
NmuCI GTSAC 9 cut(s) 382, 476, 638, 721, 970, 1228, 1576, 1828, 2300
NruI TCGCGA 1 cut(s) 2646
NsiI ATGCAT 1 cut(s) 2683
NspI RCATGY 2 cut(s) 129, 2251
PaeI GCATGC 1 cut(s) 129
PaeR7I CTCGAG 3 cut(s) 732, 1701, 2160
PaqCI CACCTGC 1 cut(s) 1048
PceI AGGCCT 1 cut(s) 517
PfeI GAWTC 6 cut(s) 137, 505, 645, 991, 1608, 2657
PkrI GCNGC 9 cut(s) 110, 131, 607, 1499, 1624, 1627, 1630, 2104, 2158
PleI GAGTC 6 cut(s) 440, 991, 1024, 1324, 1942, 2515
PpsI GAGTC 6 cut(s) 440, 991, 1024, 1324, 1942, 2515
PshAI GACNNNNGTC 2 cut(s) 1179, 1583
Psp1406I AACGTT 1 cut(s) 1396
Psp6I CCWGG 4 cut(s) 672, 1193, 2343, 2623
PspFI CCCAGC 2 cut(s) 869, 2189
PspGI CCWGG 4 cut(s) 672, 1193, 2343, 2623
PspN4I GGNNCC 8 cut(s) 679, 1030, 1443, 2349, 2439, 2532, 2772, 2799
PspPI GGNCC 6 cut(s) 272, 1028, 1301, 1442, 2035, 2797
PspXI VCTCGAGB 3 cut(s) 732, 1701, 2160
PsrI GAACNNNNNNTAC 2 cut(s) 518, 550
PstI CTGCAG 2 cut(s) 88, 1174
PstNI CAGNNNCTG 1 cut(s) 648
PsuI RGATCY 7 cut(s) 1249, 1417, 1637, 2347, 2530, 2696, 2714
PvuII CAGCTG 2 cut(s) 1655, 2336
RruI TCGCGA 1 cut(s) 2646
RsaI GTAC 6 cut(s) 623, 738, 839, 1817, 2357, 2710
RsaNI GTAC 6 cut(s) 622, 737, 838, 1816, 2356, 2709
RseI CAYNNNNRTG 4 cut(s) 746, 2211, 2593, 2735
SalI GTCGAC 1 cut(s) 668
SatI GCNGC 9 cut(s) 109, 130, 606, 1498, 1623, 1626, 1629, 2103, 2157
Sau96I GGNCC 6 cut(s) 272, 1028, 1301, 1442, 2035, 2797
ScaI AGTACT 2 cut(s) 2357, 2710
SchI GAGTC 6 cut(s) 441, 991, 1025, 1324, 1942, 2516
ScrFI CCNGG 5 cut(s) 674, 1195, 1663, 2345, 2625
SduI GDGCHC 4 cut(s) 490, 753, 1065, 2607
SfaNI GCATC 5 cut(s) 357, 718, 1326, 1640, 2690
SfcI CTRYAG 3 cut(s) 84, 1170, 1656
Sfr274I CTCGAG 3 cut(s) 732, 1701, 2160
SinI GGWCC 4 cut(s) 272, 1028, 1301, 2035
SlaI CTCGAG 3 cut(s) 732, 1701, 2160
SmiMI CAYNNNNRTG 4 cut(s) 746, 2211, 2593, 2735
SmlI CTYRAG 5 cut(s) 732, 1701, 1811, 2160, 2292
SmoI CTYRAG 5 cut(s) 732, 1701, 1811, 2160, 2292
SpeI ACTAGT 2 cut(s) 584, 1645
SphI GCATGC 1 cut(s) 129
SseBI AGGCCT 1 cut(s) 517
SsiI CCGC 3 cut(s) 422, 778, 2753
SspMI CTAG 8 cut(s) 542, 585, 764, 1298, 1646, 1761, 1820, 2651
StuI AGGCCT 1 cut(s) 517
StyD4I CCNGG 5 cut(s) 672, 1193, 1661, 2343, 2623
StyI CCWWGG 6 cut(s) 447, 1561, 1731, 1942, 2149, 2256
TaaI ACNGT 9 cut(s) 303, 370, 667, 862, 902, 1219, 1234, 1883, 2306
TaiI ACGT 4 cut(s) 270, 1098, 1399, 2464
TaqI TCGA 7 cut(s) 669, 733, 1598, 1702, 1924, 2161, 2481
TatI WGTACW 3 cut(s) 837, 2355, 2708
TfiI GAWTC 6 cut(s) 137, 505, 645, 991, 1608, 2657
TscAI CASTG 7 cut(s) 865, 907, 1237, 1419, 1888, 1920, 2311
TseFI GTSAC 9 cut(s) 382, 476, 638, 721, 970, 1228, 1576, 1828, 2300
TseI GCWGC 9 cut(s) 108, 129, 605, 1497, 1622, 1625, 1628, 2102, 2156
Tsp45I GTSAC 9 cut(s) 382, 476, 638, 721, 970, 1228, 1576, 1828, 2300
TspRI CASTG 7 cut(s) 865, 907, 1237, 1419, 1888, 1920, 2311
VneI GTGCAC 1 cut(s) 1061
VpaK11BI GGWCC 4 cut(s) 272, 1028, 1301, 2035
XagI CCTNNNNNAGG 1 cut(s) 2295
XbaI TCTAGA 1 cut(s) 541
XceI RCATGY 2 cut(s) 129, 2251
XcmI CCANNNNNNNNNTGG 2 cut(s) 1568, 2466
XhoI CTCGAG 3 cut(s) 732, 1701, 2160
XmiI GTMKAC 1 cut(s) 669
XspI CTAG 8 cut(s) 542, 585, 764, 1298, 1646, 1761, 1820, 2651
ZrmI AGTACT 2 cut(s) 2357, 2710
Zsp2I ATGCAT 1 cut(s) 2683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.