Rh2DG222700

Nodulation receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013594
Physical Location & Seq
Forward (+)
21460197 .. 21468535
8339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG222700.1

Sequence Viewer

Length: 2889 bp
ATGATGAAGCAATTAAACATTTGGGTTTTAAGACTGACAGAGTTCTTCATACTTTGCCTCTTCATTTTTAATCCATTGACTTCTGCAGATGAAGGCAGTAATTCAAGCAGCTTTGTGAGTATTGCATGCTGCTCAGAATCTAATTTTACAGATATAAGAAACATAAGTTGGGTAACAGACTTTAATGAGTTTCGTAATAGGCTAAGTTGTGAGGACAGAAAGCCAATTGCGATTGATTATGTTAACAATAAAACAAGGGTGTTTCACGTTGGTCCAAATCAGAAAAGATGTTATACCTTACCATCAAAAAAGGGTGAAGACTATCTAATAAGGGGTATTTTCGGAGGCATCTGGGGAGTGAAATTTACAGTTGCCGTTGGTGTGACCACAATCGGAGTAGTAGATACATCCGATGATTTAGCGGTTGAGGGAGTCTTTAGAGCTACCAAGGACTACACACACTTTTGCTTGGTAAGTGACGAAGGAGCACTTTATATTTCAGAGATTCAGTTGAGGCCTCTGTGGAACTTGGGCTACCATCTAGAGGACTTCTCTTCAAGTGTTCTGGAACTTCTTAAAAGGACTAGTTTTGATGATATAAGAGCAGCAAAGTTCATCAGGTACGAAGCTGACCCGAGTGACAGAATCTGGTTAACAAACTCAACTGTCGACCTGGGAACCTTCCACATAAAAGAAGAACCCAATGTCAACGTTCTGGGCGTCACAGATGCTCGAGTACCCATTCAAGTGCTCCAAACAGCTCTAGCTTCTTCAGACCGCTTGGTCATTCTTCACGATGGCCTGTCCTCAACAAACAATCACTACCTTTTGTTCCTGTACTTCTATGAAGTCAATCACACTGTTCAAGCTGGGAAGAGGGTTTTTGATGTTTACTTGAACAGTGAGCTGAGACGGCAAGGATTTGATGTTATGGGTAATGGGTCAAAGTACAGGGAGCTTGCTTTGAATGTGACAGCAAATGGGTATCTGAATCTGACTCTGGTTAAGGCGTCTGAGTCTGAAAATGGACCCATTTGCAATGCCTATGAGATTTTGCAGGTGCACCAGTTGGTTCAGGAGACTAACACAGATGACGTGGAAGTGATTTTGGAGGTGAGGAGTGAGTTGCTGGAGAATAATCAGATGAATGAAGTTTGGGAAGGGTGGACTGCAGACCCATGTCTTCCTGTTCCCTGGAATGGTATAGTTTGTGAAACTGTTAATGGTGTCACTGTCATTACCAAACTGGATCTTTCTTCTGGTATTCAGAGAGGGTCTGATGAAGAACGCAAACTTATAGGACCATTTCCTGAAAGCATCACTAAGCTGACTCACCTAAAATTCCTGAACCTCAGCAACAATGGGTTTACTGGCCAAATTCCAGAATTTCTTGAACGTTCCATGTTGATTTCAGTGGATCTGAGCCATAATAATCTTTCGGGGCCACTTCCAAATTCTCTTGCCTCACAACCAAATTTGGAAACACTGGATCTGAGCCATAATAATCTTTCGGGGCCACTTCCAAATTCTCTTGCCTCACAACCAAATTTGGAAACACTAAATTTTGGCTGCAATCCTCAGCTTACCAAAGTTAGTCCATCAACCTTCAATGGCTTAAACTTTACAACAGACCATGGAAATTGTGGTGTGACTGGTGTCAAAAAATTCTCGAAACGAGGAATCATCATTAGTGCTGCTGCTGCATCTGGATCTTTACTAGTAACAGCTGTAGCCGGGATTTTTTTTTTCATGTGGAGACAAAAGTTAATGCCTCGAGGAAAATTTGATAAGAACCCGTATCCCATGGCAAAGAATTTAATTTTCTCATTACCTAGCATGGATGATCTGATGATGAAATCAATAGCAATAGAGACATTTACTCTTGAGTACCTAGAGGCTGTCACCCAAAGTTACAAAACGCTGATAGGTGAAGGAGGTTTTGGGTCTGTTTACAGTGGCACTCTAACTGATGGTCAGGAAGTGGCAGTGAAGGTTCGATCAGCAACATCAACCCAAGGGACTCGGGAGTTTGATAATGAGCTAAATCTTCTGTCAACTATTCGGCATGAGAACCTTGTGCCTCTTCTTGGTTATTGTAACGAAAAGGACCAAGAAATTCTTGTTTATCCATTTATGTCCAATGGCTCTCTGCAAGATCGCCTATATGGGGAAGCAGCAAAAAGGAAAATTCTGGACTGGCCAACAAGACTATCAATTGCTCTTGGTGCTGCTCGAGGTTTGACACATCTGCACAATTTTGCTGGGCGTTGCATTATACATAGGGATGTGAAATCAAGTAATATACTTCTGGATCAAAGCATGTGTGCCAAGGTGGCAGACTTTGGTTTTTCAAAATATGCTCCTCAAGAAGGTGACAGTGGTGCTTCTCTGGAAGTTAGAGGGACAGCTGGATACCTGGATCCAGAGTACTACACAACCCATCATTTATCAGCAAAAAGTGATGTGTTTAGCTTTGGCGTGGTTCTACTTGAAATTGTTACTGGTCGGGAACCTCTGAACATACACAGGCCACGTCCCGAGTGGAGCTTAGTCGAATGGGCGAAACCCTATATAAGAGAGTCAAAGATTGATGAAATTGTGGATCCAAACATAAAAGGAGCATACCATGCAGAAGCTATGTGGAGGGTGGTGGAGGTAGCATTGTCATGTATTGAGCCCTTCTCTGCTTACCGACCAGGCATGGCTGACATTGTTCGCGAGCTAGAGGATTCTCTGATAATAGAGAACAATGCATCAGAATACATGAGATCCATAGAAAGTACTGGATCTAATCGCTTTTCCATAATCATGGACAGAAGGATACCGCCACCTACTCCTTCGGAACCCTCAACTATTTTGACACAAATGGCCCCTCCAGAGCCAAGATAG

Protein Analysis

962

Amino Acids

107.38

Weight (kDa)

5.34

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 49 - 354 2.1e-33 Malectin-like domain
Malectin PF11721 268 - 312 2.1e-06 Malectin domain
LRR_4 PF12799 491 - 533 9.5e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 639 - 908 3.2e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 639 - 841 2.4e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1048
AasI GACNNNNNNGTC 1 cut(s) 782
AbsI CCTCGAGG 1 cut(s) 1773
Acc36I ACCTGC 1 cut(s) 1048
AccI GTMKAC 1 cut(s) 669
AccII CGCG 1 cut(s) 2718
AciI CCGC 3 cut(s) 422, 778, 2825
AclI AACGTT 2 cut(s) 711, 1396
AcoI YGGCCR 2 cut(s) 1372, 2198
AcuI CTGAAG 1 cut(s) 756
AcyI GRCGYC 2 cut(s) 720, 1010
AfaI GTAC 7 cut(s) 623, 738, 839, 950, 1889, 2429, 2782
AfiI CCNNNNNNNGG 5 cut(s) 544, 1199, 2087, 2167, 2369
AhlI ACTAGT 2 cut(s) 584, 1717
AjiI CACGTC 2 cut(s) 1096, 2534
AjnI CCWGG 4 cut(s) 672, 1193, 2415, 2695
AjuI GAANNNNNNNTTGG 4 cut(s) 151, 183, 1923, 1955
Alw21I GWGCWC 3 cut(s) 490, 753, 1065
Alw26I GTCTC 4 cut(s) 904, 1073, 1750, 1865
Alw44I GTGCAC 1 cut(s) 1061
AlwNI CAGNNNCTG 1 cut(s) 648
Ama87I CYCGRG 6 cut(s) 634, 732, 1773, 2022, 2232, 2537
AoxI GGCC 8 cut(s) 515, 799, 1372, 1442, 1514, 2198, 2528, 2868
ApaLI GTGCAC 1 cut(s) 1061
ApeKI GCWGC 9 cut(s) 108, 129, 605, 1569, 1694, 1697, 1700, 2174, 2228
AspS9I GGNCC 7 cut(s) 272, 1028, 1301, 1442, 1514, 2107, 2869
AsuC2I CCSGG 1 cut(s) 1735
AsuHPI GGTGA 6 cut(s) 326, 1126, 1325, 1894, 1940, 2384
AvaI CYCGRG 6 cut(s) 634, 732, 1773, 2022, 2232, 2537
AvaII GGWCC 4 cut(s) 272, 1028, 1301, 2107
BaeGI GKGCMC 1 cut(s) 1065
BalI TGGCCA 2 cut(s) 1374, 2200
BamHI GGATCC 2 cut(s) 2419, 2602
BanII GRGCYC 1 cut(s) 2679
BbsI GAAGAC 2 cut(s) 324, 1175
Bbv12I GWGCWC 3 cut(s) 490, 753, 1065
BbvCI CCTCAGC 2 cut(s) 1352, 1578
BbvI GCAGC 9 cut(s) 116, 120, 617, 1556, 1681, 1684, 1687, 2186, 2215
BccI CCATC 6 cut(s) 310, 546, 791, 1606, 1964, 2448
BceAI ACGGC 2 cut(s) 359, 929
BciT130I CCWGG 4 cut(s) 674, 1195, 2417, 2697
BciVI GTATCC 3 cut(s) 1809, 2405, 2814
BcnI CCSGG 1 cut(s) 1735
BcoDI GTCTC 4 cut(s) 904, 1073, 1750, 1865
BcuI ACTAGT 2 cut(s) 584, 1717
BfaI CTAG 7 cut(s) 542, 585, 764, 1718, 1833, 1892, 2723
BfmI CTRYAG 3 cut(s) 84, 1170, 1728
BfuAI ACCTGC 1 cut(s) 1048
BfuI GTATCC 3 cut(s) 1809, 2405, 2814
BglI GCCNNNNNGGC 1 cut(s) 2333
BisI GCNGC 9 cut(s) 109, 130, 606, 1570, 1695, 1698, 1701, 2175, 2229
BlsI GCNGC 9 cut(s) 110, 131, 607, 1571, 1696, 1699, 1702, 2176, 2230
BmcAI AGTACT 2 cut(s) 2429, 2782
Bme1390I CCNGG 5 cut(s) 674, 1195, 1735, 2417, 2697
Bme18I GGWCC 4 cut(s) 272, 1028, 1301, 2107
BmeT110I CYCGRG 6 cut(s) 634, 732, 1773, 2022, 2232, 2537
BmgBI CACGTC 2 cut(s) 1096, 2534
BmgT120I GGNCC 7 cut(s) 272, 1028, 1301, 1442, 1514, 2107, 2869
BmiI GGNNCC 9 cut(s) 679, 1030, 1443, 1515, 2421, 2511, 2604, 2844, 2871
BmrFI CCNGG 5 cut(s) 674, 1195, 1735, 2417, 2697
BmsI GCATC 5 cut(s) 357, 718, 1326, 1712, 2762
BoxI GACNNNNGTC 2 cut(s) 1179, 1655
BpiI GAAGAC 2 cut(s) 324, 1175
BplI GAGNNNNNCTC 4 cut(s) 536, 568, 2666, 2698
BpmI CTGGAG 2 cut(s) 1151, 2859
Bpu10I CCTNAGC 2 cut(s) 1352, 1578
BpuEI CTTGAG 2 cut(s) 1904, 2349
BpuMI CCSGG 1 cut(s) 1735
BsaHI GRCGYC 2 cut(s) 720, 1010
BsaJI CCNNGG 7 cut(s) 447, 673, 1193, 1633, 1803, 2014, 2328
Bsc4I CCNNNNNNNGG 5 cut(s) 544, 1199, 2087, 2167, 2369
Bse1I ACTGG 8 cut(s) 1066, 1251, 1375, 1491, 1657, 2201, 2506, 2788
Bse3DI GCAATG 1 cut(s) 1045
BseBI CCWGG 4 cut(s) 674, 1195, 2417, 2697
BseDI CCNNGG 7 cut(s) 447, 673, 1193, 1633, 1803, 2014, 2328
BseGI GGATG 3 cut(s) 407, 1846, 2290
BseLI CCNNNNNNNGG 5 cut(s) 544, 1199, 2087, 2167, 2369
BseMI GCAATG 1 cut(s) 1045
BseMII CTCAG 7 cut(s) 147, 899, 1005, 1366, 1412, 1484, 1592
BseNI ACTGG 8 cut(s) 1066, 1251, 1375, 1491, 1657, 2201, 2506, 2788
BseRI GAGGAG 2 cut(s) 1132, 2352
BseSI GKGCMC 1 cut(s) 1065
BseXI GCAGC 9 cut(s) 116, 120, 617, 1556, 1681, 1684, 1687, 2186, 2215
BseYI CCCAGC 2 cut(s) 869, 2261
BsgI GTGCAG 1 cut(s) 2234
Bsh1236I CGCG 1 cut(s) 2718
BshFI GGCC 8 cut(s) 517, 801, 1374, 1444, 1516, 2200, 2530, 2870
BsiHKAI GWGCWC 3 cut(s) 490, 753, 1065
BsiHKCI CYCGRG 6 cut(s) 634, 732, 1773, 2022, 2232, 2537
BsiSI CCGG 1 cut(s) 1734
BslFI GGGAC 3 cut(s) 2032, 2416, 2520
BslI CCNNNNNNNGG 5 cut(s) 544, 1199, 2087, 2167, 2369
BsmAI GTCTC 4 cut(s) 904, 1073, 1750, 1865
BsmBI CGTCTC 1 cut(s) 904
BsmFI GGGAC 3 cut(s) 2032, 2416, 2520
BsnI GGCC 8 cut(s) 517, 801, 1374, 1444, 1516, 2200, 2530, 2870
BsoBI CYCGRG 6 cut(s) 634, 732, 1773, 2022, 2232, 2537
Bsp1286I GDGCHC 4 cut(s) 490, 753, 1065, 2679
Bsp19I CCATGG 2 cut(s) 1633, 1803
Bsp68I TCGCGA 1 cut(s) 2718
BspACI CCGC 3 cut(s) 422, 778, 2825
BspANI GGCC 8 cut(s) 517, 801, 1374, 1444, 1516, 2200, 2530, 2870
BspCNI CTCAG 7 cut(s) 146, 900, 1006, 1365, 1413, 1485, 1591
BspFNI CGCG 1 cut(s) 2718
BspLI GGNNCC 9 cut(s) 679, 1030, 1443, 1515, 2421, 2511, 2604, 2844, 2871
BspMAI CTGCAG 2 cut(s) 88, 1174
BspMI ACCTGC 1 cut(s) 1048
BsrDI GCAATG 1 cut(s) 1045
BsrI ACTGG 8 cut(s) 1066, 1251, 1375, 1491, 1657, 2201, 2506, 2788
BssECI CCNNGG 7 cut(s) 447, 673, 1193, 1633, 1803, 2014, 2328
BssNI GRCGYC 2 cut(s) 720, 1010
BssT1I CCWWGG 5 cut(s) 447, 1633, 1803, 2014, 2328
Bst2UI CCWGG 4 cut(s) 674, 1195, 2417, 2697
Bst4CI ACNGT 8 cut(s) 370, 667, 862, 902, 1219, 1234, 1955, 2378
Bst6I CTCTTC 4 cut(s) 65, 559, 869, 2088
BstACI GRCGYC 2 cut(s) 720, 1010
BstAPI GCANNNNNTGC 1 cut(s) 2627
BstC8I GCNNGC 3 cut(s) 127, 960, 2720
BstDSI CCRYGG 2 cut(s) 1633, 1803
BstENI CCTNNNNNAGG 1 cut(s) 2367
BstF5I GGATG 3 cut(s) 407, 1846, 2290
BstFNI CGCG 1 cut(s) 2718
BstMAI GTCTC 4 cut(s) 904, 1073, 1750, 1865
BstMWI GCNNNNNNNGC 5 cut(s) 913, 1700, 2225, 2333, 2627
BstNI CCWGG 4 cut(s) 674, 1195, 2417, 2697
BstNSI RCATGY 2 cut(s) 129, 2323
BstPAI GACNNNNGTC 2 cut(s) 1179, 1655
BstSCI CCNGG 5 cut(s) 672, 1193, 1733, 2415, 2695
BstSFI CTRYAG 3 cut(s) 84, 1170, 1728
BstSLI GKGCMC 1 cut(s) 1065
BstUI CGCG 1 cut(s) 2718
BstV1I GCAGC 9 cut(s) 116, 120, 617, 1556, 1681, 1684, 1687, 2186, 2215
BstV2I GAAGAC 2 cut(s) 324, 1175
BstX2I RGATCY 8 cut(s) 1249, 1417, 1489, 1709, 2419, 2602, 2768, 2786
BstXI CCANNNNNNTGG 1 cut(s) 2809
BstYI RGATCY 8 cut(s) 1249, 1417, 1489, 1709, 2419, 2602, 2768, 2786
BsuI GTATCC 3 cut(s) 1809, 2405, 2814
BsuRI GGCC 8 cut(s) 517, 801, 1374, 1444, 1516, 2200, 2530, 2870
BtgI CCRYGG 2 cut(s) 1633, 1803
BtrI CACGTC 2 cut(s) 1096, 2534
BtsCI GGATG 3 cut(s) 407, 1846, 2290
BtsI GCAGTG 1 cut(s) 1992
BtsIMutI CAGTG 8 cut(s) 858, 907, 1230, 1419, 1484, 1960, 1992, 2383
BtuMI TCGCGA 1 cut(s) 2718
BveI ACCTGC 1 cut(s) 1048
Cac8I GCNNGC 3 cut(s) 127, 960, 2720
CaiI CAGNNNCTG 1 cut(s) 648
Cfr13I GGNCC 7 cut(s) 272, 1028, 1301, 1442, 1514, 2107, 2869
CseI GACGC 2 cut(s) 709, 999
Csp6I GTAC 7 cut(s) 622, 737, 838, 949, 1888, 2428, 2781
CviQI GTAC 7 cut(s) 622, 737, 838, 949, 1888, 2428, 2781
DrdI GACNNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 782
EaeI YGGCCR 2 cut(s) 1372, 2198
Eam1104I CTCTTC 4 cut(s) 65, 559, 869, 2088
EarI CTCTTC 4 cut(s) 65, 559, 869, 2088
Eco130I CCWWGG 5 cut(s) 447, 1633, 1803, 2014, 2328
Eco147I AGGCCT 1 cut(s) 517
Eco24I GRGCYC 1 cut(s) 2679
Eco47I GGWCC 4 cut(s) 272, 1028, 1301, 2107
Eco57I CTGAAG 1 cut(s) 756
Eco88I CYCGRG 6 cut(s) 634, 732, 1773, 2022, 2232, 2537
EcoNI CCTNNNNNAGG 1 cut(s) 2367
EcoRII CCWGG 4 cut(s) 672, 1193, 2415, 2695
EcoT14I CCWWGG 5 cut(s) 447, 1633, 1803, 2014, 2328
EcoT22I ATGCAT 1 cut(s) 2755
EcoT38I GRGCYC 1 cut(s) 2679
ErhI CCWWGG 5 cut(s) 447, 1633, 1803, 2014, 2328
Esp3I CGTCTC 1 cut(s) 904
FalI AAGNNNNNCTT 4 cut(s) 474, 506, 2103, 2135
FaqI GGGAC 3 cut(s) 2032, 2416, 2520
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 9 cut(s) 109, 130, 606, 1570, 1695, 1698, 1701, 2175, 2229
FokI GGATG 3 cut(s) 394, 1853, 2297
FriOI GRGCYC 1 cut(s) 2679
Fsp4HI GCNGC 9 cut(s) 109, 130, 606, 1570, 1695, 1698, 1701, 2175, 2229
FspBI CTAG 7 cut(s) 542, 585, 764, 1718, 1833, 1892, 2723
GluI GCNGC 9 cut(s) 109, 130, 606, 1570, 1695, 1698, 1701, 2175, 2229
GsaI CCCAGC 2 cut(s) 873, 2265
GsuI CTGGAG 2 cut(s) 1151, 2859
HaeIII GGCC 8 cut(s) 517, 801, 1374, 1444, 1516, 2200, 2530, 2870
HapII CCGG 1 cut(s) 1734
HgaI GACGC 2 cut(s) 709, 999
Hin1I GRCGYC 2 cut(s) 720, 1010
HincII GTYRAC 5 cut(s) 244, 654, 670, 709, 2055
HindII GTYRAC 5 cut(s) 244, 654, 670, 709, 2055
HpaI GTTAAC 2 cut(s) 244, 654
HpaII CCGG 1 cut(s) 1734
HphI GGTGA 6 cut(s) 326, 1126, 1325, 1894, 1940, 2384
HpyCH4III ACNGT 8 cut(s) 370, 667, 862, 902, 1219, 1234, 1955, 2378
HpyCH4IV ACGT 5 cut(s) 267, 711, 1095, 1396, 2533
HpyF10VI GCNNNNNNNGC 5 cut(s) 913, 1700, 2225, 2333, 2627
HpySE526I ACGT 5 cut(s) 267, 711, 1095, 1396, 2533
Hsp92I GRCGYC 2 cut(s) 720, 1010
KspAI GTTAAC 2 cut(s) 244, 654
LmnI GCTCC 6 cut(s) 485, 756, 955, 2365, 2544, 2618
Lsp1109I GCAGC 9 cut(s) 116, 120, 617, 1556, 1681, 1684, 1687, 2186, 2215
LweI GCATC 5 cut(s) 357, 718, 1326, 1712, 2762
MaeI CTAG 7 cut(s) 542, 585, 764, 1718, 1833, 1892, 2723
MaeII ACGT 5 cut(s) 267, 711, 1095, 1396, 2533
MfeI CAATTG 2 cut(s) 225, 2214
MflI RGATCY 8 cut(s) 1249, 1417, 1489, 1709, 2419, 2602, 2768, 2786
MhlI GDGCHC 4 cut(s) 490, 753, 1065, 2679
MlsI TGGCCA 2 cut(s) 1374, 2200
MluNI TGGCCA 2 cut(s) 1374, 2200
MlyI GAGTC 6 cut(s) 441, 991, 1025, 1324, 2014, 2588
Mox20I TGGCCA 2 cut(s) 1374, 2200
Mph1103I ATGCAT 1 cut(s) 2755
MscI TGGCCA 2 cut(s) 1374, 2200
MslI CAYNNNNRTG 4 cut(s) 746, 2283, 2665, 2807
Msp20I TGGCCA 2 cut(s) 1374, 2200
MspA1I CMGCKG 2 cut(s) 1727, 2408
MspI CCGG 1 cut(s) 1734
MspR9I CCNGG 5 cut(s) 674, 1195, 1735, 2417, 2697
MunI CAATTG 2 cut(s) 225, 2214
MvaI CCWGG 4 cut(s) 674, 1195, 2417, 2697
MvnI CGCG 1 cut(s) 2718
MwoI GCNNNNNNNGC 5 cut(s) 913, 1700, 2225, 2333, 2627
NciI CCSGG 1 cut(s) 1735
NcoI CCATGG 2 cut(s) 1633, 1803
NlaIV GGNNCC 9 cut(s) 679, 1030, 1443, 1515, 2421, 2511, 2604, 2844, 2871
NmuCI GTSAC 9 cut(s) 382, 476, 638, 721, 970, 1228, 1648, 1900, 2372
NruI TCGCGA 1 cut(s) 2718
NsiI ATGCAT 1 cut(s) 2755
NspI RCATGY 2 cut(s) 129, 2323
PaeI GCATGC 1 cut(s) 129
PaeR7I CTCGAG 3 cut(s) 732, 1773, 2232
PaqCI CACCTGC 1 cut(s) 1048
PceI AGGCCT 1 cut(s) 517
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PfeI GAWTC 6 cut(s) 137, 505, 645, 991, 1680, 2729
PkrI GCNGC 9 cut(s) 110, 131, 607, 1571, 1696, 1699, 1702, 2176, 2230
PleI GAGTC 6 cut(s) 440, 991, 1024, 1324, 2014, 2587
PpsI GAGTC 6 cut(s) 440, 991, 1024, 1324, 2014, 2587
PshAI GACNNNNGTC 2 cut(s) 1179, 1655
Psp1406I AACGTT 2 cut(s) 711, 1396
Psp6I CCWGG 4 cut(s) 672, 1193, 2415, 2695
PspFI CCCAGC 2 cut(s) 869, 2261
PspGI CCWGG 4 cut(s) 672, 1193, 2415, 2695
PspN4I GGNNCC 9 cut(s) 679, 1030, 1443, 1515, 2421, 2511, 2604, 2844, 2871
PspPI GGNCC 7 cut(s) 272, 1028, 1301, 1442, 1514, 2107, 2869
PspXI VCTCGAGB 3 cut(s) 732, 1773, 2232
PsrI GAACNNNNNNTAC 2 cut(s) 518, 550
PstI CTGCAG 2 cut(s) 88, 1174
PstNI CAGNNNCTG 1 cut(s) 648
PsuI RGATCY 8 cut(s) 1249, 1417, 1489, 1709, 2419, 2602, 2768, 2786
PvuII CAGCTG 2 cut(s) 1727, 2408
RruI TCGCGA 1 cut(s) 2718
RsaI GTAC 7 cut(s) 623, 738, 839, 950, 1889, 2429, 2782
RsaNI GTAC 7 cut(s) 622, 737, 838, 949, 1888, 2428, 2781
RseI CAYNNNNRTG 4 cut(s) 746, 2283, 2665, 2807
SalI GTCGAC 1 cut(s) 668
SatI GCNGC 9 cut(s) 109, 130, 606, 1570, 1695, 1698, 1701, 2175, 2229
Sau96I GGNCC 7 cut(s) 272, 1028, 1301, 1442, 1514, 2107, 2869
ScaI AGTACT 2 cut(s) 2429, 2782
SchI GAGTC 6 cut(s) 441, 991, 1025, 1324, 2014, 2588
ScrFI CCNGG 5 cut(s) 674, 1195, 1735, 2417, 2697
SduI GDGCHC 4 cut(s) 490, 753, 1065, 2679
SfaNI GCATC 5 cut(s) 357, 718, 1326, 1712, 2762
SfcI CTRYAG 3 cut(s) 84, 1170, 1728
Sfr274I CTCGAG 3 cut(s) 732, 1773, 2232
SinI GGWCC 4 cut(s) 272, 1028, 1301, 2107
SlaI CTCGAG 3 cut(s) 732, 1773, 2232
SmiMI CAYNNNNRTG 4 cut(s) 746, 2283, 2665, 2807
SmlI CTYRAG 5 cut(s) 732, 1773, 1883, 2232, 2364
SmoI CTYRAG 5 cut(s) 732, 1773, 1883, 2232, 2364
SpeI ACTAGT 2 cut(s) 584, 1717
SphI GCATGC 1 cut(s) 129
SseBI AGGCCT 1 cut(s) 517
SsiI CCGC 3 cut(s) 422, 778, 2825
SspMI CTAG 7 cut(s) 542, 585, 764, 1718, 1833, 1892, 2723
StuI AGGCCT 1 cut(s) 517
StyD4I CCNGG 5 cut(s) 672, 1193, 1733, 2415, 2695
StyI CCWWGG 5 cut(s) 447, 1633, 1803, 2014, 2328
TaaI ACNGT 8 cut(s) 370, 667, 862, 902, 1219, 1234, 1955, 2378
TaiI ACGT 5 cut(s) 270, 714, 1098, 1399, 2536
TaqI TCGA 7 cut(s) 669, 733, 1670, 1774, 1996, 2233, 2553
TatI WGTACW 4 cut(s) 837, 948, 2427, 2780
TfiI GAWTC 6 cut(s) 137, 505, 645, 991, 1680, 2729
TscAI CASTG 8 cut(s) 865, 907, 1237, 1419, 1491, 1960, 1992, 2383
TseFI GTSAC 9 cut(s) 382, 476, 638, 721, 970, 1228, 1648, 1900, 2372
TseI GCWGC 9 cut(s) 108, 129, 605, 1569, 1694, 1697, 1700, 2174, 2228
Tsp45I GTSAC 9 cut(s) 382, 476, 638, 721, 970, 1228, 1648, 1900, 2372
TspRI CASTG 8 cut(s) 865, 907, 1237, 1419, 1491, 1960, 1992, 2383
VneI GTGCAC 1 cut(s) 1061
VpaK11BI GGWCC 4 cut(s) 272, 1028, 1301, 2107
XagI CCTNNNNNAGG 1 cut(s) 2367
XbaI TCTAGA 1 cut(s) 541
XceI RCATGY 2 cut(s) 129, 2323
XcmI CCANNNNNNNNNTGG 2 cut(s) 1640, 2538
XhoI CTCGAG 3 cut(s) 732, 1773, 2232
XmiI GTMKAC 1 cut(s) 669
XspI CTAG 7 cut(s) 542, 585, 764, 1718, 1833, 1892, 2723
ZrmI AGTACT 2 cut(s) 2429, 2782
Zsp2I ATGCAT 1 cut(s) 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.