Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009617

Physical Location & Seq

Genomic Coordinates Reverse (-)

31545917 .. 31554578

Gene Structure 8662 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1312700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGAATTGTCTCCAGAATCTGACAGGATCCTCTGTTTTTCCTCTCCGGAGCTTCGGAAGCAATCCCACGAGGAGGCCTTCCTTCTTTATCGCAACTCTGGGAACGATGAGCTGCATCGATCTACCCAGAAGCATGGCTGTGAAGGCAATTTCTGGTTCCACCTCTAGTGCTGAGACGAGTGGTGCTGAAGCGAAAGAGGAAAAGTCTGAGATATATAGTAATAACATGACAGAAGCCATGGGTGCTGTCTTGACCTATAGGCATGAGCTAGGTATGAACTACGACTTCATACGTCCAGATCTGATTGTGGGGTCTTGCCTACAGACACCTAAAGATGTTGACAAACTTCGAAGCATCGGCGTGAAGACCATTTTTTGCTTGCAACAAGATTCAGATTTGGAATATTTTAGTGTTGACATCGGTGACATTCGAGAGTATGCCAACACATTTGATGACATTGAACACTTGCGTGCTGAAATAAGGGACTTTGATTCATATGATCTACGTCTCCGGCTTCCTGTTGTTGTTAGCAAATTATATAAAGCCATAAATCGAAATGGAGGTGTCACTTATGTGCATTGCACTGCTGGACTTGGAAGAGCTCCTGCAGCTGCGTTGGCTTACATGTTCTGGGTACAGGGATACAAACTTACAGATGCTGTCAATTTACTGCTGAGCAAACGTGCGTGCTTCCCAAAACTGGATGCTATAAAAAATGCTACTGCAGATATTCTTACAAGCATGACAAAAAGGGTTGTCACATTGACTTGGGGAGGTGATAATTGCTCTACAGTGGAAATCTCAGGACTTGACATTGGATGGGGTCAGAGGATTCCTTTAGAGTTTAATAAGGAACAGGGTTCGTGGATTCTCAAGAGGGAATTGCCGGAAGGACGATATGAATATAAATACGTCATTGATGGTGGATGGACGCACAATAAGCATGAGCCTTTCACCTCTCCCAACAAGGATGGCCATGTCAACAATTATGTTCATGTTGTTGAGGACGACCCTAACAGTGAAAGTGCGGCCATACGGAAGAGGATGACCGGTGATGATCCTCATCTTACAACCGAGGAACGACTGAAAATAAGACGGTTCCTCGAATCTTGTCCCAGTGATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001871 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0030246 GO:0030247 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0097367 GO:1901575 GO:2001066

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMPK1_CBM DSPc

Protein Analysis

376

Amino Acids

42.04

Weight (kDa)

5.84

Isoelectric Point (pI)

53.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PTP-SAK	PF22784	101 - 218	6.4e-06	Swiss Army Knife protein, DSP-PTPase phosphatase domain
DSPc	PF00782	149 - 231	8e-10	Dual specificity phosphatase, catalytic domain
AMPK1_CBM	PF16561	253 - 337	2.3e-18	Glycogen recognition site of AMP-activated protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009617)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52180 AT3G52180 AT3G52180 AT3G52180
fragaria_vesca	FvH4_7g06850 FvH4_7g06850
malus_domestica	MD15G1312700.v1.1
prunus_persica	Prupe.2G096100_v2.0.a1 Prupe.2G096100_v2.0.a1
pyrus_communis	pycom01g06680 pycom15g27630
rosa_chinensis	RchiOBHm_Chr1g0336591
rosa_laevigata	RLG00000029350
rosa_multiflora	Rmu_sc0002196.1_g000002 Rmu_sc0031975.1_g000001
rosa_roxburghii	Rroxscaffold_4G00315480
rosa_rugosa	Rorug01G0126700
rosa_samantha	Rh1AG147900 Rh1BG115700 Rh1CG139300 Rh1DG154300
rosa_wichuraiana	Rw1G011940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	45
AciI	CCGC	1 cut(s)	1028
AclWI	GGATC	3 cut(s)	21, 34, 1052
AcoI	YGGCCR	2 cut(s)	973, 1029
AcuI	CTGAAG	1 cut(s)	207
AfaI	GTAC	1 cut(s)	636
AfiI	CCNNNNNNNGG	2 cut(s)	72, 700
AflIII	ACRYGT	1 cut(s)	624
AgeI	ACCGGT	1 cut(s)	1049
AgsI	TTSAA	1 cut(s)	461
AleI	CACNNNNGTG	2 cut(s)	468, 572
AluBI	AGCT	5 cut(s)	51, 111, 268, 602, 611
AluI	AGCT	5 cut(s)	51, 111, 268, 602, 611
Alw21I	GWGCWC	1 cut(s)	604
Alw26I	GTCTC	3 cut(s)	14, 167, 512
AlwI	GGATC	3 cut(s)	21, 34, 1052
AlwNI	CAGNNNCTG	2 cut(s)	19, 659
Aor13HI	TCCGGA	1 cut(s)	45
AoxI	GGCC	3 cut(s)	74, 973, 1029
ApeKI	GCWGC	3 cut(s)	111, 608, 611
AsiGI	ACCGGT	1 cut(s)	1049
AsuHPI	GGTGA	4 cut(s)	434, 788, 946, 1064
AsuII	TTCGAA	1 cut(s)	349
BalI	TGGCCA	1 cut(s)	975
BamHI	GGATCC	1 cut(s)	26
BanII	GRGCYC	1 cut(s)	604
BauI	CACGAG	1 cut(s)	67
BbsI	GAAGAC	1 cut(s)	371
Bbv12I	GWGCWC	1 cut(s)	604
BbvI	GCAGC	3 cut(s)	98, 598, 620
BccI	CCATC	4 cut(s)	813, 914, 921, 965
BciVI	GTATCC	1 cut(s)	635
BcoDI	GTCTC	3 cut(s)	14, 167, 512
BfaI	CTAG	2 cut(s)	165, 269
BfmI	CTRYAG	5 cut(s)	256, 320, 606, 723, 789
BfuI	GTATCC	1 cut(s)	635
BglII	AGATCT	1 cut(s)	298
BisI	GCNGC	4 cut(s)	112, 609, 612, 1029
BlpI	GCTNAGC	1 cut(s)	674
BlsI	GCNGC	4 cut(s)	113, 610, 613, 1030
BmiI	GGNNCC	3 cut(s)	28, 157, 1100
BmrI	ACTGGG	1 cut(s)	1110
BmsI	GCATC	4 cut(s)	123, 363, 646, 694
BmuI	ACTGGG	1 cut(s)	1110
BpiI	GAAGAC	1 cut(s)	371
Bpu1102I	GCTNAGC	1 cut(s)	674
Bpu14I	TTCGAA	1 cut(s)	349
BpuEI	CTTGAG	1 cut(s)	857
Bsa29I	ATCGAT	1 cut(s)	117
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaJI	CCNNGG	2 cut(s)	237, 1074
BsaWI	WCCGGW	2 cut(s)	45, 1049
Bsc4I	CCNNNNNNNGG	2 cut(s)	72, 700
Bse118I	RCCGGY	1 cut(s)	1049
Bse1I	ACTGG	2 cut(s)	705, 1116
Bse3DI	GCAATG	1 cut(s)	577
Bse8I	GATNNNNATC	1 cut(s)	1062
BseAI	TCCGGA	1 cut(s)	45
BseCI	ATCGAT	1 cut(s)	117
BseDI	CCNNGG	2 cut(s)	237, 1074
BseGI	GGATG	5 cut(s)	709, 824, 932, 976, 1050
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	2 cut(s)	72, 700
BseMI	GCAATG	1 cut(s)	577
BseMII	CTCAG	4 cut(s)	162, 198, 665, 816
BseNI	ACTGG	2 cut(s)	705, 1116
BseRI	GAGGAG	1 cut(s)	85
BseXI	GCAGC	3 cut(s)	98, 598, 620
BshFI	GGCC	3 cut(s)	76, 975, 1031
BshTI	ACCGGT	1 cut(s)	1049
BshVI	ATCGAT	1 cut(s)	117
BsiHKAI	GWGCWC	1 cut(s)	604
BsiSI	CCGG	4 cut(s)	46, 511, 887, 1050
BslFI	GGGAC	2 cut(s)	497, 1098
BslI	CCNNNNNNNGG	2 cut(s)	72, 700
BsmAI	GTCTC	3 cut(s)	14, 167, 512
BsmBI	CGTCTC	2 cut(s)	167, 512
BsmFI	GGGAC	2 cut(s)	497, 1098
BsnI	GGCC	3 cut(s)	76, 975, 1031
Bsp119I	TTCGAA	1 cut(s)	349
Bsp1286I	GDGCHC	1 cut(s)	604
Bsp13I	TCCGGA	1 cut(s)	45
Bsp143I	GATC	5 cut(s)	26, 118, 298, 499, 1057
Bsp1720I	GCTNAGC	1 cut(s)	674
Bsp19I	CCATGG	1 cut(s)	237
BspACI	CCGC	1 cut(s)	1028
BspANI	GGCC	3 cut(s)	76, 975, 1031
BspCNI	CTCAG	4 cut(s)	163, 199, 666, 815
BspDI	ATCGAT	1 cut(s)	117
BspEI	TCCGGA	1 cut(s)	45
BspLI	GGNNCC	3 cut(s)	28, 157, 1100
BspMAI	CTGCAG	2 cut(s)	610, 727
BspPI	GGATC	3 cut(s)	21, 34, 1052
BspQI	GCTCTTC	1 cut(s)	592
BspT104I	TTCGAA	1 cut(s)	349
BsrDI	GCAATG	1 cut(s)	577
BsrFI	RCCGGY	1 cut(s)	1049
BsrI	ACTGG	2 cut(s)	705, 1116
BssAI	RCCGGY	1 cut(s)	1049
BssECI	CCNNGG	2 cut(s)	237, 1074
BssMI	GATC	5 cut(s)	26, 118, 298, 499, 1057
BssSI	CACGAG	1 cut(s)	67
BssT1I	CCWWGG	1 cut(s)	237
Bst2BI	CACGAG	1 cut(s)	67
Bst4CI	ACNGT	3 cut(s)	793, 1019, 1098
Bst6I	CTCTTC	2 cut(s)	592, 1034
BstBI	TTCGAA	1 cut(s)	349
BstC8I	GCNNGC	3 cut(s)	380, 471, 688
BstDEI	CTNAG	4 cut(s)	171, 207, 674, 802
BstDSI	CCRYGG	1 cut(s)	237
BstF5I	GGATG	5 cut(s)	709, 824, 932, 976, 1050
BstKTI	GATC	5 cut(s)	29, 121, 301, 502, 1060
BstMAI	GTCTC	3 cut(s)	14, 167, 512
BstMBI	GATC	5 cut(s)	26, 118, 298, 499, 1057
BstMWI	GCNNNNNNNGC	6 cut(s)	57, 143, 242, 608, 617, 940
BstNSI	RCATGY	1 cut(s)	628
BstSFI	CTRYAG	5 cut(s)	256, 320, 606, 723, 789
BstV1I	GCAGC	3 cut(s)	98, 598, 620
BstV2I	GAAGAC	1 cut(s)	371
BstX2I	RGATCY	2 cut(s)	26, 298
BstXI	CCANNNNNNTGG	1 cut(s)	133
BstYI	RGATCY	2 cut(s)	26, 298
Bsu15I	ATCGAT	1 cut(s)	117
BsuI	GTATCC	1 cut(s)	635
BsuRI	GGCC	3 cut(s)	76, 975, 1031
BsuTUI	ATCGAT	1 cut(s)	117
BtgI	CCRYGG	1 cut(s)	237
BtsCI	GGATG	5 cut(s)	709, 824, 932, 976, 1050
BtsI	GCAGTG	1 cut(s)	582
BtsIMutI	CAGTG	4 cut(s)	582, 798, 1024, 1123
Cac8I	GCNNGC	3 cut(s)	380, 471, 688
CaiI	CAGNNNCTG	2 cut(s)	19, 659
Cfr10I	RCCGGY	1 cut(s)	1049
ClaI	ATCGAT	1 cut(s)	117
CseI	GACGC	1 cut(s)	940
Csp6I	GTAC	1 cut(s)	635
CspAI	ACCGGT	1 cut(s)	1049
CviAII	CATG	9 cut(s)	133, 226, 238, 263, 625, 742, 944, 977, 995
CviQI	GTAC	1 cut(s)	635
DdeI	CTNAG	4 cut(s)	171, 207, 674, 802
DpnI	GATC	5 cut(s)	28, 120, 300, 501, 1059
DpnII	GATC	5 cut(s)	26, 118, 298, 499, 1057
EaeI	YGGCCR	2 cut(s)	973, 1029
Eam1104I	CTCTTC	2 cut(s)	592, 1034
EarI	CTCTTC	2 cut(s)	592, 1034
Ecl136II	GAGCTC	1 cut(s)	602
Eco130I	CCWWGG	1 cut(s)	237
Eco147I	AGGCCT	1 cut(s)	76
Eco24I	GRGCYC	1 cut(s)	604
Eco53kI	GAGCTC	1 cut(s)	602
Eco57I	CTGAAG	1 cut(s)	207
EcoICRI	GAGCTC	1 cut(s)	602
EcoT14I	CCWWGG	1 cut(s)	237
EcoT38I	GRGCYC	1 cut(s)	604
ErhI	CCWWGG	1 cut(s)	237
Esp3I	CGTCTC	2 cut(s)	167, 512
FaeI	CATG	9 cut(s)	136, 229, 241, 266, 628, 745, 947, 980, 998
FaqI	GGGAC	2 cut(s)	497, 1098
FatI	CATG	9 cut(s)	132, 225, 237, 262, 624, 741, 943, 976, 994
FauNDI	CATATG	1 cut(s)	496
Fnu4HI	GCNGC	4 cut(s)	112, 609, 612, 1029
FokI	GGATG	5 cut(s)	716, 831, 939, 983, 1057
FriOI	GRGCYC	1 cut(s)	604
Fsp4HI	GCNGC	4 cut(s)	112, 609, 612, 1029
FspBI	CTAG	2 cut(s)	165, 269
GluI	GCNGC	4 cut(s)	112, 609, 612, 1029
HaeIII	GGCC	3 cut(s)	76, 975, 1031
HapII	CCGG	4 cut(s)	46, 511, 887, 1050
HgaI	GACGC	1 cut(s)	940
Hin1II	CATG	9 cut(s)	136, 229, 241, 266, 628, 745, 947, 980, 998
HincII	GTYRAC	3 cut(s)	340, 415, 982
HindII	GTYRAC	3 cut(s)	340, 415, 982
HinfI	GANTC	6 cut(s)	16, 389, 491, 832, 868, 1106
HpaII	CCGG	4 cut(s)	46, 511, 887, 1050
HphI	GGTGA	4 cut(s)	434, 788, 946, 1064
Hpy166II	GTNNAC	3 cut(s)	340, 415, 982
Hpy188I	TCNGA	6 cut(s)	21, 56, 208, 303, 394, 828
Hpy188III	TCNNGA	7 cut(s)	13, 46, 250, 296, 431, 804, 874
Hpy8I	GTNNAC	3 cut(s)	340, 415, 982
HpyAV	CCTTC	4 cut(s)	87, 91, 136, 884
HpyCH4III	ACNGT	3 cut(s)	793, 1019, 1098
HpyCH4IV	ACGT	4 cut(s)	292, 505, 682, 912
HpyCH4V	TGCA	6 cut(s)	114, 382, 577, 582, 608, 725
HpyF10VI	GCNNNNNNNGC	6 cut(s)	57, 143, 242, 608, 617, 940
HpyF3I	CTNAG	4 cut(s)	171, 207, 674, 802
HpySE526I	ACGT	4 cut(s)	292, 505, 682, 912
Hsp92II	CATG	9 cut(s)	136, 229, 241, 266, 628, 745, 947, 980, 998
Kpn2I	TCCGGA	1 cut(s)	45
Kzo9I	GATC	5 cut(s)	26, 118, 298, 499, 1057
LguI	GCTCTTC	1 cut(s)	592
LmnI	GCTCC	2 cut(s)	48, 607
Lsp1109I	GCAGC	3 cut(s)	98, 598, 620
LweI	GCATC	4 cut(s)	123, 363, 646, 694
MaeI	CTAG	2 cut(s)	165, 269
MaeII	ACGT	4 cut(s)	292, 505, 682, 912
MaeIII	GTNAC	3 cut(s)	422, 565, 757
MalI	GATC	5 cut(s)	28, 120, 300, 501, 1059
MboI	GATC	5 cut(s)	26, 118, 298, 499, 1057
MboII	GAAGA	3 cut(s)	376, 609, 1051
MflI	RGATCY	2 cut(s)	26, 298
MhlI	GDGCHC	1 cut(s)	604
MlsI	TGGCCA	1 cut(s)	975
MluCI	AATT	7 cut(s)	4, 147, 533, 664, 781, 881, 985
MluNI	TGGCCA	1 cut(s)	975
Mox20I	TGGCCA	1 cut(s)	975
MroI	TCCGGA	1 cut(s)	45
MscI	TGGCCA	1 cut(s)	975
MseI	TTAA	1 cut(s)	846
MslI	CAYNNNNRTG	4 cut(s)	137, 359, 468, 572
Msp20I	TGGCCA	1 cut(s)	975
MspA1I	CMGCKG	1 cut(s)	611
MspI	CCGG	4 cut(s)	46, 511, 887, 1050
MwoI	GCNNNNNNNGC	6 cut(s)	57, 143, 242, 608, 617, 940
NcoI	CCATGG	1 cut(s)	237
NdeI	CATATG	1 cut(s)	496
NdeII	GATC	5 cut(s)	26, 118, 298, 499, 1057
NlaIII	CATG	9 cut(s)	136, 229, 241, 266, 628, 745, 947, 980, 998
NlaIV	GGNNCC	3 cut(s)	28, 157, 1100
NmuCI	GTSAC	3 cut(s)	422, 565, 757
NspI	RCATGY	1 cut(s)	628
NspV	TTCGAA	1 cut(s)	349
OliI	CACNNNNGTG	2 cut(s)	468, 572
PceI	AGGCCT	1 cut(s)	76
PciI	ACATGT	1 cut(s)	624
PciSI	GCTCTTC	1 cut(s)	592
PfeI	GAWTC	6 cut(s)	16, 389, 491, 832, 868, 1106
PinAI	ACCGGT	1 cut(s)	1049
PkrI	GCNGC	4 cut(s)	113, 610, 613, 1030
PscI	ACATGT	1 cut(s)	624
Psp124BI	GAGCTC	1 cut(s)	604
PspN4I	GGNNCC	3 cut(s)	28, 157, 1100
PstI	CTGCAG	2 cut(s)	610, 727
PstNI	CAGNNNCTG	2 cut(s)	19, 659
PsuI	RGATCY	2 cut(s)	26, 298
PvuII	CAGCTG	1 cut(s)	611
RsaI	GTAC	1 cut(s)	636
RsaNI	GTAC	1 cut(s)	635
RseI	CAYNNNNRTG	4 cut(s)	137, 359, 468, 572
SacI	GAGCTC	1 cut(s)	604
SapI	GCTCTTC	1 cut(s)	592
SaqAI	TTAA	1 cut(s)	846
SatI	GCNGC	4 cut(s)	112, 609, 612, 1029
Sau3AI	GATC	5 cut(s)	26, 118, 298, 499, 1057
SduI	GDGCHC	1 cut(s)	604
SfaNI	GCATC	4 cut(s)	123, 363, 646, 694
SfcI	CTRYAG	5 cut(s)	256, 320, 606, 723, 789
SfuI	TTCGAA	1 cut(s)	349
SmiMI	CAYNNNNRTG	4 cut(s)	137, 359, 468, 572
SmlI	CTYRAG	1 cut(s)	872
SmoI	CTYRAG	1 cut(s)	872
Sse9I	AATT	7 cut(s)	4, 147, 533, 664, 781, 881, 985
SseBI	AGGCCT	1 cut(s)	76
SsiI	CCGC	1 cut(s)	1028
SspI	AATATT	1 cut(s)	404
SspMI	CTAG	2 cut(s)	165, 269
SstI	GAGCTC	1 cut(s)	604
StuI	AGGCCT	1 cut(s)	76
StyI	CCWWGG	1 cut(s)	237
TaaI	ACNGT	3 cut(s)	793, 1019, 1098
TaiI	ACGT	4 cut(s)	295, 508, 685, 915
TaqI	TCGA	5 cut(s)	117, 349, 430, 553, 1104
TasI	AATT	7 cut(s)	4, 147, 533, 664, 781, 881, 985
TauI	GCSGC	1 cut(s)	1031
TfiI	GAWTC	6 cut(s)	16, 389, 491, 832, 868, 1106
Tru1I	TTAA	1 cut(s)	846
Tru9I	TTAA	1 cut(s)	846
TscAI	CASTG	4 cut(s)	589, 798, 1024, 1123
TseFI	GTSAC	3 cut(s)	422, 565, 757
TseI	GCWGC	3 cut(s)	111, 608, 611
Tsp45I	GTSAC	3 cut(s)	422, 565, 757
TspDTI	ATGAA	6 cut(s)	17, 277, 290, 483, 915, 983
TspGWI	ACGGA	1 cut(s)	1051
TspRI	CASTG	4 cut(s)	589, 798, 1024, 1123
XceI	RCATGY	1 cut(s)	628
XspI	CTAG	2 cut(s)	165, 269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.