Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006058

Physical Location & Seq

Genomic Coordinates Reverse (-)

32648618 .. 32649682

Gene Structure 1065 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1316800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1065 bp

ATGCCAGATACGAAAACGACGAGCTTCAATCTCCTCTTCCTCTTCTTGCTCGCCTATCCTATGCCGGCCAATGCCCAGACCCCCGAGTTTCAGAACAACGGCTATGGCTACATCGCCAATTTGAACTCTTCAATGGCGCTCGTCAGTGTCTTCCTCGTCTTTGCCTTCTTCTCCGTGGGCTTCCTCTCCGTCTACTTTCGCCGCTGCATCGAGTCCACACGCATGGAAGCCTCCGGAACTCGAGCTCGCGCCGCCTCCGTAGGTTTCCGCCGGCGAGGACTGGACCCGGCGGTGATGGAGACTTTCCCGATCCTCGTCTACTCCGCCGTCAAGGATCTCAAGATTGGCAAGGGAGCTCTGGAGTGCGCCGTGTGCTTGAGCGAGTTCGACGACTTCGAAACGCTGCGTTTGCTTCCCAAATGCGATCACGTCTTCCATCCAGATTGCATCGACCCCTGGTTAGCGGCTCACGTGACGTGTCCGGTTTGCCGCTCCAACCTCACGCGAAACACCGATTTAAATGTCGAAGATTCGAGTTCCAGGAGTCCGATTGAAGTCGGCGAGGTACGAAACGACGCCGTTGTGCTCAATGTGGATGAAAATCCAAGCGAGGGCCCACAAGCGGTTGAGAATGGTAAACCACGAACGAGATCCGGAATATGTGGAAAGTTTCCGAGATCGCACTCGACGGGTCACTCGCTGAGCCGACCCGGATTGAACACGGAGAGGTACACGTTGAGACTGCCGGAGGAGGTGAGGAAGCAGCTGGTGTCGAGCGGGAACAGTAAGCTGAGGCGTTCGACTAGCTACGACGTCCTTTTGCGCTGCGGCCTCGGGAGTTCGAGGAAGGGCTATAGGTGTGGCCGCTGTGAAGGGAAAAGCAGCGTGGATAATCAAACCGGTTGGGTCGACCCGTGGATGATGTCGCCGTCGTTTTTACCTAGAGGAGGTGACGGAAATGCGGTGTCTGGGAAGACGCCGTTAATGTCTGTTAAAATGCCGTTGGATTGTCTTCATGTCAAGGCGGAGAGTTCTAAAGAATCGATATCTCGGCTGCCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

355

Amino Acids

39.01

Weight (kDa)

8.8

Isoelectric Point (pI)

51.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	121 - 164	3.5e-13	Ring finger domain
zf-RING_11	PF17123	121 - 150	3.6e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	121 - 163	1.3e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	122 - 163	5.3e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	134 - 164	1.7e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09110 AT4G09120 AT4G09130
fragaria_vesca	FvH4_1g20110
malus_domestica	MD15G1316800.v1.1
prunus_persica	Prupe.6G195100_v2.0.a1
pyrus_communis	pycom01g06440 pycom15g27950
rosa_chinensis	RchiOBHm_Chr2g0112141 RchiOBHm_Chr7g0233001
rosa_laevigata	RLG00000001306 RLG00000017954
rosa_multiflora	Rmu_sc0000360.1_g000026 Rmu_sc0001294.1_g000027 Rmu_ssc0000018.1_g000029
rosa_roxburghii	Rroxscaffold_2G00131730
rosa_rugosa	Rorug02G0178700
rosa_samantha	Rh2AG230700 Rh2BG244000 Rh2CG235300 Rh2DG238600 Rh7AG432600 Rh7BG405400 Rh7CG451900 Rh7DG421400
rosa_wichuraiana	Rw2G017900 Rw7G035670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	816
AccBSI	CCGCTC	2 cut(s)	492, 777
AccI	GTMKAC	3 cut(s)	192, 318, 909
AccII	CGCG	2 cut(s)	249, 505
AccIII	TCCGGA	2 cut(s)	233, 653
AclWI	GGATC	3 cut(s)	304, 342, 645
AcoI	YGGCCR	2 cut(s)	66, 862
AcvI	CACGTG	1 cut(s)	472
AcyI	GRCGYC	3 cut(s)	576, 813, 977
AfaI	GTAC	2 cut(s)	567, 731
AfiI	CCNNNNNNNGG	3 cut(s)	611, 622, 947
AflIII	ACRYGT	2 cut(s)	476, 732
AgeI	ACCGGT	1 cut(s)	899
AgsI	TTSAA	5 cut(s)	28, 124, 132, 554, 718
AjiI	CACGTC	2 cut(s)	430, 477
AjnI	CCWGG	2 cut(s)	455, 539
AluBI	AGCT	6 cut(s)	24, 245, 356, 766, 790, 807
AluI	AGCT	6 cut(s)	24, 245, 356, 766, 790, 807
Alw21I	GWGCWC	3 cut(s)	247, 358, 588
Alw26I	GTCTC	2 cut(s)	293, 733
AlwI	GGATC	3 cut(s)	304, 342, 645
Ama87I	CYCGRG	3 cut(s)	83, 240, 833
Aor13HI	TCCGGA	2 cut(s)	233, 653
AoxI	GGCC	4 cut(s)	66, 613, 829, 862
ApaI	GGGCCC	1 cut(s)	617
ApeKI	GCWGC	6 cut(s)	204, 403, 763, 825, 882, 1054
AsiGI	ACCGGT	1 cut(s)	899
AspLEI	GCGC	4 cut(s)	139, 251, 368, 825
AspS9I	GGNCC	3 cut(s)	283, 613, 614
AsuC2I	CCSGG	2 cut(s)	287, 711
AsuHPI	GGTGA	3 cut(s)	304, 766, 962
AsuII	TTCGAA	1 cut(s)	396
AvaI	CYCGRG	3 cut(s)	83, 240, 833
AvaII	GGWCC	1 cut(s)	283
BaeGI	GKGCMC	1 cut(s)	617
BanII	GRGCYC	3 cut(s)	247, 358, 617
BbrPI	CACGTG	1 cut(s)	472
BbsI	GAAGAC	4 cut(s)	142, 424, 980, 1004
Bbv12I	GWGCWC	3 cut(s)	247, 358, 588
BbvCI	CCTCAGC	1 cut(s)	791
BbvI	GCAGC	6 cut(s)	191, 390, 775, 812, 894, 1041
BccI	CCATC	2 cut(s)	289, 444
BceAI	ACGGC	7 cut(s)	115, 311, 353, 563, 913, 964, 985
BciT130I	CCWGG	2 cut(s)	457, 541
BcnI	CCSGG	2 cut(s)	287, 711
BcoDI	GTCTC	2 cut(s)	293, 733
BfaI	CTAG	2 cut(s)	804, 942
BfmI	CTRYAG	1 cut(s)	853
BfoI	RGCGCY	1 cut(s)	140
BlpI	GCTNAGC	1 cut(s)	701
Bme1390I	CCNGG	4 cut(s)	287, 457, 541, 711
Bme18I	GGWCC	1 cut(s)	283
BmeT110I	CYCGRG	3 cut(s)	83, 240, 833
BmgBI	CACGTC	2 cut(s)	430, 477
BmgT120I	GGNCC	3 cut(s)	283, 613, 614
BmiI	GGNNCC	2 cut(s)	285, 615
BmrFI	CCNGG	4 cut(s)	287, 457, 541, 711
BmsI	GCATC	2 cut(s)	216, 456
BpiI	GAAGAC	4 cut(s)	142, 424, 980, 1004
BpmI	CTGGAG	1 cut(s)	380
Bpu10I	CCTNAGC	1 cut(s)	791
Bpu1102I	GCTNAGC	1 cut(s)	701
Bpu14I	TTCGAA	1 cut(s)	396
BpuEI	CTTGAG	2 cut(s)	323, 397
BpuMI	CCSGG	2 cut(s)	287, 711
Bsa29I	ATCGAT	1 cut(s)	1043
BsaAI	YACGTR	1 cut(s)	472
BsaBI	GATNNNNATC	1 cut(s)	600
BsaHI	GRCGYC	3 cut(s)	576, 813, 977
BsaJI	CCNNGG	4 cut(s)	174, 455, 832, 914
BsaWI	WCCGGW	4 cut(s)	233, 481, 653, 899
Bsc4I	CCNNNNNNNGG	3 cut(s)	611, 622, 947
Bse118I	RCCGGY	3 cut(s)	64, 270, 899
Bse1I	ACTGG	1 cut(s)	285
Bse8I	GATNNNNATC	1 cut(s)	600
BseAI	TCCGGA	2 cut(s)	233, 653
BseBI	CCWGG	2 cut(s)	457, 541
BseCI	ATCGAT	1 cut(s)	1043
BseDI	CCNNGG	4 cut(s)	174, 455, 832, 914
BseGI	GGATG	3 cut(s)	436, 601, 924
BseJI	GATNNNNATC	1 cut(s)	600
BseLI	CCNNNNNNNGG	3 cut(s)	611, 622, 947
BseMII	CTCAG	2 cut(s)	692, 782
BseNI	ACTGG	1 cut(s)	285
BseRI	GAGGAG	3 cut(s)	23, 764, 960
BseSI	GKGCMC	1 cut(s)	617
BseXI	GCAGC	6 cut(s)	191, 390, 775, 812, 894, 1041
Bsh1236I	CGCG	2 cut(s)	249, 505
BshFI	GGCC	4 cut(s)	68, 615, 831, 864
BshTI	ACCGGT	1 cut(s)	899
BshVI	ATCGAT	1 cut(s)	1043
BsiHKAI	GWGCWC	3 cut(s)	247, 358, 588
BsiHKCI	CYCGRG	3 cut(s)	83, 240, 833
BsiSI	CCGG	9 cut(s)	65, 234, 271, 287, 482, 654, 711, 746, 900
BslI	CCNNNNNNNGG	3 cut(s)	611, 622, 947
BsmAI	GTCTC	2 cut(s)	293, 733
BsnI	GGCC	4 cut(s)	68, 615, 831, 864
BsoBI	CYCGRG	3 cut(s)	83, 240, 833
Bsp119I	TTCGAA	1 cut(s)	396
Bsp120I	GGGCCC	1 cut(s)	613
Bsp1286I	GDGCHC	4 cut(s)	247, 358, 588, 617
Bsp13I	TCCGGA	2 cut(s)	233, 653
Bsp143I	GATC	5 cut(s)	309, 334, 424, 650, 677
Bsp1720I	GCTNAGC	1 cut(s)	701
BspANI	GGCC	4 cut(s)	68, 615, 831, 864
BspCNI	CTCAG	2 cut(s)	693, 783
BspDI	ATCGAT	1 cut(s)	1043
BspEI	TCCGGA	2 cut(s)	233, 653
BspFNI	CGCG	2 cut(s)	249, 505
BspLI	GGNNCC	2 cut(s)	285, 615
BspPI	GGATC	3 cut(s)	304, 342, 645
BspT104I	TTCGAA	1 cut(s)	396
BsrBI	CCGCTC	2 cut(s)	492, 777
BsrFI	RCCGGY	3 cut(s)	64, 270, 899
BsrI	ACTGG	1 cut(s)	285
BssAI	RCCGGY	3 cut(s)	64, 270, 899
BssECI	CCNNGG	4 cut(s)	174, 455, 832, 914
BssMI	GATC	5 cut(s)	309, 334, 424, 650, 677
BssNI	GRCGYC	3 cut(s)	576, 813, 977
Bst2UI	CCWGG	2 cut(s)	457, 541
Bst4CI	ACNGT	1 cut(s)	785
Bst6I	CTCTTC	3 cut(s)	41, 47, 133
BstACI	GRCGYC	3 cut(s)	576, 813, 977
BstBAI	YACGTR	1 cut(s)	472
BstBI	TTCGAA	1 cut(s)	396
BstC8I	GCNNGC	4 cut(s)	51, 66, 247, 272
BstDEI	CTNAG	2 cut(s)	701, 791
BstDSI	CCRYGG	2 cut(s)	174, 914
BstENI	CCTNNNNNAGG	1 cut(s)	945
BstF5I	GGATG	3 cut(s)	436, 601, 924
BstFNI	CGCG	2 cut(s)	249, 505
BstH2I	RGCGCY	1 cut(s)	140
BstHHI	GCGC	4 cut(s)	139, 251, 368, 825
BstKTI	GATC	5 cut(s)	312, 337, 427, 653, 680
BstMAI	GTCTC	2 cut(s)	293, 733
BstMBI	GATC	5 cut(s)	309, 334, 424, 650, 677
BstMWI	GCNNNNNNNGC	3 cut(s)	251, 372, 409
BstNI	CCWGG	2 cut(s)	457, 541
BstSCI	CCNGG	4 cut(s)	285, 455, 539, 709
BstSFI	CTRYAG	1 cut(s)	853
BstSLI	GKGCMC	1 cut(s)	617
BstUI	CGCG	2 cut(s)	249, 505
BstV1I	GCAGC	6 cut(s)	191, 390, 775, 812, 894, 1041
BstV2I	GAAGAC	4 cut(s)	142, 424, 980, 1004
BstX2I	RGATCY	2 cut(s)	334, 650
BstXI	CCANNNNNNTGG	1 cut(s)	223
BstYI	RGATCY	2 cut(s)	334, 650
Bsu15I	ATCGAT	1 cut(s)	1043
BsuRI	GGCC	4 cut(s)	68, 615, 831, 864
BsuTUI	ATCGAT	1 cut(s)	1043
BtgI	CCRYGG	2 cut(s)	174, 914
BtgZI	GCGATG	1 cut(s)	97
BtrI	CACGTC	2 cut(s)	430, 477
BtsCI	GGATG	3 cut(s)	436, 601, 924
BtsIMutI	CAGTG	1 cut(s)	151
Cac8I	GCNNGC	4 cut(s)	51, 66, 247, 272
CfoI	GCGC	4 cut(s)	139, 251, 368, 825
Cfr10I	RCCGGY	3 cut(s)	64, 270, 899
Cfr13I	GGNCC	3 cut(s)	283, 613, 614
ClaI	ATCGAT	1 cut(s)	1043
CseI	GACGC	2 cut(s)	584, 985
Csp6I	GTAC	2 cut(s)	566, 730
CspAI	ACCGGT	1 cut(s)	899
CviAII	CATG	2 cut(s)	223, 1016
CviQI	GTAC	2 cut(s)	566, 730
DdeI	CTNAG	2 cut(s)	701, 791
DpnI	GATC	5 cut(s)	311, 336, 426, 652, 679
DpnII	GATC	5 cut(s)	309, 334, 424, 650, 677
DraI	TTTAAA	1 cut(s)	519
EaeI	YGGCCR	2 cut(s)	66, 862
Eam1104I	CTCTTC	3 cut(s)	41, 47, 133
EarI	CTCTTC	3 cut(s)	41, 47, 133
EciI	GGCGGA	3 cut(s)	257, 313, 1040
Ecl136II	GAGCTC	2 cut(s)	245, 356
Eco24I	GRGCYC	3 cut(s)	247, 358, 617
Eco32I	GATATC	1 cut(s)	1047
Eco47I	GGWCC	1 cut(s)	283
Eco53kI	GAGCTC	2 cut(s)	245, 356
Eco72I	CACGTG	1 cut(s)	472
Eco88I	CYCGRG	3 cut(s)	83, 240, 833
EcoICRI	GAGCTC	2 cut(s)	245, 356
EcoNI	CCTNNNNNAGG	1 cut(s)	945
EcoO109I	RGGNCCY	1 cut(s)	613
EcoRII	CCWGG	2 cut(s)	455, 539
EcoRV	GATATC	1 cut(s)	1047
EcoT38I	GRGCYC	3 cut(s)	247, 358, 617
FaeI	CATG	2 cut(s)	226, 1019
FaiI	YATR	6 cut(s)	62, 105, 224, 661, 855, 1017
FatI	CATG	2 cut(s)	222, 1015
FauI	CCCGC	1 cut(s)	770
FblI	GTMKAC	3 cut(s)	192, 318, 909
FokI	GGATG	3 cut(s)	423, 608, 931
FriOI	GRGCYC	3 cut(s)	247, 358, 617
FspBI	CTAG	2 cut(s)	804, 942
GlaI	GCGC	4 cut(s)	138, 250, 367, 824
GsuI	CTGGAG	1 cut(s)	380
HaeII	RGCGCY	1 cut(s)	140
HaeIII	GGCC	4 cut(s)	68, 615, 831, 864
HapII	CCGG	9 cut(s)	65, 234, 271, 287, 482, 654, 711, 746, 900
HgaI	GACGC	2 cut(s)	584, 985
HhaI	GCGC	4 cut(s)	139, 251, 368, 825
Hin1I	GRCGYC	3 cut(s)	576, 813, 977
Hin1II	CATG	2 cut(s)	226, 1019
Hin6I	GCGC	4 cut(s)	137, 249, 366, 823
HinP1I	GCGC	4 cut(s)	137, 249, 366, 823
HincII	GTYRAC	1 cut(s)	910
HindII	GTYRAC	1 cut(s)	910
HinfI	GANTC	4 cut(s)	212, 530, 544, 1040
HpaII	CCGG	9 cut(s)	65, 234, 271, 287, 482, 654, 711, 746, 900
HphI	GGTGA	3 cut(s)	304, 766, 962
Hpy166II	GTNNAC	6 cut(s)	193, 216, 319, 638, 732, 910
Hpy188I	TCNGA	3 cut(s)	93, 549, 675
Hpy188III	TCNNGA	7 cut(s)	234, 307, 340, 359, 440, 654, 835
Hpy8I	GTNNAC	6 cut(s)	193, 216, 319, 638, 732, 910
Hpy99I	CGWCG	6 cut(s)	22, 392, 578, 691, 815, 934
HpyAV	CCTTC	3 cut(s)	175, 841, 866
HpyCH4III	ACNGT	1 cut(s)	785
HpyCH4IV	ACGT	5 cut(s)	429, 471, 476, 734, 813
HpyCH4V	TGCA	2 cut(s)	207, 447
HpyF10VI	GCNNNNNNNGC	3 cut(s)	251, 372, 409
HpyF3I	CTNAG	2 cut(s)	701, 791
HpySE526I	ACGT	5 cut(s)	429, 471, 476, 734, 813
Hsp92I	GRCGYC	3 cut(s)	576, 813, 977
Hsp92II	CATG	2 cut(s)	226, 1019
HspAI	GCGC	4 cut(s)	137, 249, 366, 823
Kpn2I	TCCGGA	2 cut(s)	233, 653
KroI	GCCGGC	2 cut(s)	64, 270
KroNI	GCCGGC	2 cut(s)	66, 272
Kzo9I	GATC	5 cut(s)	309, 334, 424, 650, 677
LmnI	GCTCC	2 cut(s)	353, 497
Lsp1109I	GCAGC	6 cut(s)	191, 390, 775, 812, 894, 1041
LweI	GCATC	2 cut(s)	216, 456
MaeI	CTAG	2 cut(s)	804, 942
MaeII	ACGT	5 cut(s)	429, 471, 476, 734, 813
MaeIII	GTNAC	3 cut(s)	472, 692, 950
MalI	GATC	5 cut(s)	311, 336, 426, 652, 679
MbiI	CCGCTC	2 cut(s)	492, 777
MboI	GATC	5 cut(s)	309, 334, 424, 650, 677
MboII	GAAGA	9 cut(s)	28, 34, 120, 142, 160, 424, 539, 985, 1004
MflI	RGATCY	2 cut(s)	334, 650
MhlI	GDGCHC	4 cut(s)	247, 358, 588, 617
MluCI	AATT	1 cut(s)	118
MlyI	GAGTC	2 cut(s)	221, 553
MmeI	TCCRAC	2 cut(s)	519, 984
MreI	CGCCGGCG	1 cut(s)	270
MroI	TCCGGA	2 cut(s)	233, 653
MroNI	GCCGGC	2 cut(s)	64, 270
MseI	TTAA	3 cut(s)	518, 983, 993
MslI	CAYNNNNRTG	1 cut(s)	221
MspA1I	CMGCKG	3 cut(s)	204, 766, 867
MspI	CCGG	9 cut(s)	65, 234, 271, 287, 482, 654, 711, 746, 900
MspR9I	CCNGG	4 cut(s)	287, 457, 541, 711
MvaI	CCWGG	2 cut(s)	457, 541
MvnI	CGCG	2 cut(s)	249, 505
MwoI	GCNNNNNNNGC	3 cut(s)	251, 372, 409
NaeI	GCCGGC	2 cut(s)	66, 272
NciI	CCSGG	2 cut(s)	287, 711
NdeII	GATC	5 cut(s)	309, 334, 424, 650, 677
NgoMIV	GCCGGC	2 cut(s)	64, 270
NlaIII	CATG	2 cut(s)	226, 1019
NlaIV	GGNNCC	2 cut(s)	285, 615
NmeAIII	GCCGAG	1 cut(s)	1030
NmuCI	GTSAC	3 cut(s)	472, 692, 950
NspV	TTCGAA	1 cut(s)	396
PaeR7I	CTCGAG	1 cut(s)	240
PcsI	WCGNNNNNNNCGW	2 cut(s)	17, 393
PdiI	GCCGGC	2 cut(s)	66, 272
PfeI	GAWTC	2 cut(s)	530, 1040
PfoI	TCCNGGA	1 cut(s)	539
PinAI	ACCGGT	1 cut(s)	899
PleI	GAGTC	2 cut(s)	220, 552
PmaCI	CACGTG	1 cut(s)	472
PmlI	CACGTG	1 cut(s)	472
PpsI	GAGTC	2 cut(s)	220, 552
Ppu21I	YACGTR	1 cut(s)	472
Psp124BI	GAGCTC	2 cut(s)	247, 358
Psp6I	CCWGG	2 cut(s)	455, 539
PspCI	CACGTG	1 cut(s)	472
PspGI	CCWGG	2 cut(s)	455, 539
PspN4I	GGNNCC	2 cut(s)	285, 615
PspOMI	GGGCCC	1 cut(s)	613
PspPI	GGNCC	3 cut(s)	283, 613, 614
PspXI	VCTCGAGB	1 cut(s)	240
PsuI	RGATCY	2 cut(s)	334, 650
PvuII	CAGCTG	1 cut(s)	766
RsaI	GTAC	2 cut(s)	567, 731
RsaNI	GTAC	2 cut(s)	566, 730
RseI	CAYNNNNRTG	1 cut(s)	221
SacI	GAGCTC	2 cut(s)	247, 358
SalI	GTCGAC	1 cut(s)	908
SaqAI	TTAA	3 cut(s)	518, 983, 993
Sau3AI	GATC	5 cut(s)	309, 334, 424, 650, 677
Sau96I	GGNCC	3 cut(s)	283, 613, 614
SchI	GAGTC	2 cut(s)	221, 553
ScrFI	CCNGG	4 cut(s)	287, 457, 541, 711
SduI	GDGCHC	4 cut(s)	247, 358, 588, 617
SfaNI	GCATC	2 cut(s)	216, 456
SfcI	CTRYAG	1 cut(s)	853
Sfr274I	CTCGAG	1 cut(s)	240
SfuI	TTCGAA	1 cut(s)	396
SgrAI	CRCCGGYG	1 cut(s)	270
SinI	GGWCC	1 cut(s)	283
SlaI	CTCGAG	1 cut(s)	240
SmiI	ATTTAAAT	1 cut(s)	519
SmiMI	CAYNNNNRTG	1 cut(s)	221
SmlI	CTYRAG	3 cut(s)	240, 338, 376
SmoI	CTYRAG	3 cut(s)	240, 338, 376
Sse9I	AATT	1 cut(s)	118
SspMI	CTAG	2 cut(s)	804, 942
SstI	GAGCTC	2 cut(s)	247, 358
StyD4I	CCNGG	4 cut(s)	285, 455, 539, 709
SwaI	ATTTAAAT	1 cut(s)	519
TaaI	ACNGT	1 cut(s)	785
TaiI	ACGT	5 cut(s)	432, 474, 479, 737, 816
TasI	AATT	1 cut(s)	118
TauI	GCSGC	6 cut(s)	204, 254, 467, 492, 831, 867
TfiI	GAWTC	2 cut(s)	530, 1040
Tru1I	TTAA	3 cut(s)	518, 983, 993
Tru9I	TTAA	3 cut(s)	518, 983, 993
TscAI	CASTG	1 cut(s)	151
TseFI	GTSAC	3 cut(s)	472, 692, 950
TseI	GCWGC	6 cut(s)	204, 403, 763, 825, 882, 1054
Tsp45I	GTSAC	3 cut(s)	472, 692, 950
TspDTI	ATGAA	2 cut(s)	612, 1004
TspGWI	ACGGA	5 cut(s)	163, 178, 247, 737, 969
TspRI	CASTG	1 cut(s)	151
VpaK11BI	GGWCC	1 cut(s)	283
XagI	CCTNNNNNAGG	1 cut(s)	945
XhoI	CTCGAG	1 cut(s)	240
XmiI	GTMKAC	3 cut(s)	192, 318, 909
XspI	CTAG	2 cut(s)	804, 942
ZraI	GACGTC	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.