Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0006058

Physical Location & Seq

Genomic Coordinates Forward (+)

6763567 .. 6764828

Gene Structure 1262 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g06440.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGTCACACCATATAACATGGAGCTTCAATCTTCTCTTCCTCTTCTTGATCGCCTATCCTACGCCGGCCAATGCCCAGACCGCTGAGTTTCACAACAACGGCTATGGCTACATCGCCAATTTGAACTCTTCAATGGCGGTCGTTATTGTCTTCCTCGTCTTCGCCTTCTTCTTGATGGGCTTCCTCTCCATCTACATTCGCCGCTGCGCTGAGTCCACTGACATGGAATCCGTCCGAACTCGAGCTCCGGCCGCCGTAGGAGCTTCCAGCAGGCGAGGCCTGGACCCGGCGGTGATCCAGACTTTCCCGATCCTCGTCTACTCCGCCGTCAAGGATCTAAAGATCGGCAAGGGAGCTCTGGAGTGCGCGGTGTGCTTGAGCGAGTTTGACGACTTCGAAACGCTGCGTTTGCTTCCTAAATGCGACCACGCCTTCCATCCAGATTGCATCGACGCCTGGCTCGCCGCTCACGTGACGTGTCCGGTTTGCCGCGCCAACCTCACGTGCGCCGAGTCGAACTCGCGAGCGACCCAGCCAAACGCCGATTTAAACCCGGAAGATTCGAATTCGAGGAGTCCGATTGAAATCCGGCAGGTACCAAACGACGCCATTGTGCTCAATGTGGATGAAAACCAAAGAGGGGGCCCAGAAGCAGTTGAGGTAGGGCACCCACGTAGGAGATCAGGAAAGTTCCTTCCTGATCTTTCGAGATCGCACTCGACGGGTCACTCGCTGATCCGACCCGGATTGGACACCGAGCGGTACACGTTAAGACTACCGGAGAAGGTGAGGGCGCAGGTAGTTTCGAGCGGCAACGGCAAGCTGACACGTTCGACTAGCTACGACATCGTTTTGCGCCGCCTCGGGAGTTCGAGGAAGGGCTATAGGTACGGCGGCGGTGAAGGGAAAAGCAGTGTGGATAATCAAACCGGTTCGACCCATGGGTCGTTAAAACTCCTAAGGGTGGGGGTGACGGAAATTTGGTGTCTGGGAAGACGCCGTTACAGTCTGAAAATGCCGTTGGATTGTCTTAAGGTTAGAACTTGGCTGCTGAAGAAATCAGCTGCAGTTCCTACAGAGGACCAATCACAGTTGGACACGTGTTCAAGTTATGATAAAAATTTTAATTACTTGGATACATGTCCAGTTGTGATTGGTCAGCAGCATGAGTTGCTGCTAATTTTTCAGCAACCAAGTTTTGTTCTTATCTTAATGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

407

Amino Acids

45.06

Weight (kDa)

8.67

Isoelectric Point (pI)

49.88

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09110 AT4G09120 AT4G09130
fragaria_vesca	FvH4_1g20110
malus_domestica	MD15G1316800.v1.1
prunus_persica	Prupe.6G195100_v2.0.a1
pyrus_communis	pycom01g06440 pycom15g27950
rosa_chinensis	RchiOBHm_Chr2g0112141 RchiOBHm_Chr7g0233001
rosa_laevigata	RLG00000001306 RLG00000017954
rosa_multiflora	Rmu_sc0000360.1_g000026 Rmu_sc0001294.1_g000027 Rmu_ssc0000018.1_g000029
rosa_roxburghii	Rroxscaffold_2G00131730
rosa_rugosa	Rorug02G0178700
rosa_samantha	Rh2AG230700 Rh2BG244000 Rh2CG235300 Rh2DG238600 Rh7AG432600 Rh7BG405400 Rh7CG451900 Rh7DG421400
rosa_wichuraiana	Rw2G017900 Rw7G035670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	943
Acc36I	ACCTGC	2 cut(s)	583, 787
Acc65I	GGTACC	1 cut(s)	595
AccB1I	GGYRCC	2 cut(s)	595, 666
AccBSI	CCGCTC	3 cut(s)	467, 760, 810
AccI	GTMKAC	1 cut(s)	318
AccII	CGCG	3 cut(s)	368, 492, 523
AclWI	GGATC	4 cut(s)	289, 304, 342, 730
AcoI	YGGCCR	2 cut(s)	66, 249
AcsI	RAATTY	3 cut(s)	565, 978, 1120
AcuI	CTGAAG	1 cut(s)	1073
AcvI	CACGTG	3 cut(s)	472, 504, 1101
AcyI	GRCGYC	3 cut(s)	453, 606, 997
AfaI	GTAC	3 cut(s)	597, 764, 890
AfiI	CCNNNNNNNGG	3 cut(s)	286, 640, 964
AflII	CTTAAG	1 cut(s)	1031
AflIII	ACRYGT	6 cut(s)	476, 765, 827, 1098, 1100, 1139
AgeI	ACCGGT	1 cut(s)	929
AgsI	TTSAA	5 cut(s)	28, 124, 132, 584, 1107
AjiI	CACGTC	1 cut(s)	477
AjnI	CCWGG	2 cut(s)	279, 455
AjuI	GAANNNNNNNTTGG	2 cut(s)	1004, 1036
AloI	GAACNNNNNNTCC	2 cut(s)	229, 261
AluBI	AGCT	7 cut(s)	24, 245, 263, 356, 823, 840, 1064
AluI	AGCT	7 cut(s)	24, 245, 263, 356, 823, 840, 1064
Alw21I	GWGCWC	3 cut(s)	247, 358, 618
AlwI	GGATC	4 cut(s)	289, 304, 342, 730
Ama87I	CYCGRG	2 cut(s)	240, 863
AoxI	GGCC	4 cut(s)	66, 249, 277, 643
ApaI	GGGCCC	1 cut(s)	647
ApeKI	GCWGC	6 cut(s)	204, 403, 1048, 1064, 1162, 1174
ApoI	RAATTY	3 cut(s)	565, 978, 1120
AsiGI	ACCGGT	1 cut(s)	929
Asp718I	GGTACC	1 cut(s)	595
AspLEI	GCGC	6 cut(s)	209, 368, 494, 509, 796, 858
AspS9I	GGNCC	4 cut(s)	283, 643, 644, 1081
AsuC2I	CCSGG	3 cut(s)	287, 554, 744
AsuHPI	GGTGA	4 cut(s)	304, 799, 911, 982
AsuII	TTCGAA	2 cut(s)	396, 563
AvaI	CYCGRG	2 cut(s)	240, 863
AvaII	GGWCC	2 cut(s)	283, 1081
AxyI	CCTNAGG	1 cut(s)	959
BaeGI	GKGCMC	2 cut(s)	647, 669
BanI	GGYRCC	2 cut(s)	595, 666
BanII	GRGCYC	3 cut(s)	247, 358, 647
BbrPI	CACGTG	3 cut(s)	472, 504, 1101
BbsI	GAAGAC	3 cut(s)	142, 151, 1000
Bbv12I	GWGCWC	3 cut(s)	247, 358, 618
BbvI	GCAGC	6 cut(s)	191, 390, 1035, 1051, 1161, 1174
BccI	CCATC	3 cut(s)	169, 197, 444
BceAI	ACGGC	7 cut(s)	115, 239, 311, 832, 907, 984, 1003
BciT130I	CCWGG	2 cut(s)	281, 457
BciVI	GTATCC	1 cut(s)	1129
BcnI	CCSGG	3 cut(s)	287, 554, 744
BfaI	CTAG	1 cut(s)	837
BfmI	CTRYAG	3 cut(s)	883, 1065, 1074
BfrI	CTTAAG	1 cut(s)	1031
BfuAI	ACCTGC	2 cut(s)	583, 787
BfuI	GTATCC	1 cut(s)	1129
Bme1390I	CCNGG	5 cut(s)	281, 287, 457, 554, 744
Bme18I	GGWCC	2 cut(s)	283, 1081
BmeT110I	CYCGRG	2 cut(s)	240, 863
BmgBI	CACGTC	1 cut(s)	477
BmgT120I	GGNCC	4 cut(s)	283, 643, 644, 1081
BmiI	GGNNCC	5 cut(s)	285, 597, 644, 645, 668
BmrFI	CCNGG	5 cut(s)	281, 287, 457, 554, 744
BmsI	GCATC	1 cut(s)	456
BpiI	GAAGAC	3 cut(s)	142, 151, 1000
BplI	GAGNNNNNCTC	2 cut(s)	503, 535
BpmI	CTGGAG	1 cut(s)	380
Bpu14I	TTCGAA	2 cut(s)	396, 563
BpuEI	CTTGAG	1 cut(s)	397
BpuMI	CCSGG	3 cut(s)	287, 554, 744
BsaAI	YACGTR	4 cut(s)	472, 504, 674, 1101
BsaBI	GATNNNNATC	1 cut(s)	584
BsaHI	GRCGYC	3 cut(s)	453, 606, 997
BsaJI	CCNNGG	2 cut(s)	862, 940
BsaWI	WCCGGW	3 cut(s)	481, 778, 929
BsaXI	ACNNNNNCTCC	2 cut(s)	229, 259
Bsc4I	CCNNNNNNNGG	3 cut(s)	286, 640, 964
Bse118I	RCCGGY	2 cut(s)	64, 929
Bse1I	ACTGG	1 cut(s)	1145
Bse21I	CCTNAGG	1 cut(s)	959
Bse8I	GATNNNNATC	1 cut(s)	584
BseBI	CCWGG	2 cut(s)	281, 457
BseDI	CCNNGG	2 cut(s)	862, 940
BseGI	GGATG	2 cut(s)	436, 631
BseJI	GATNNNNATC	1 cut(s)	584
BseLI	CCNNNNNNNGG	3 cut(s)	286, 640, 964
BseMII	CTCAG	2 cut(s)	75, 201
BseNI	ACTGG	1 cut(s)	1145
BseRI	GAGGAG	1 cut(s)	586
BseSI	GKGCMC	2 cut(s)	647, 669
BseX3I	CGGCCG	1 cut(s)	249
BseXI	GCAGC	6 cut(s)	191, 390, 1035, 1051, 1161, 1174
BseYI	CCCAGC	1 cut(s)	531
Bsh1236I	CGCG	3 cut(s)	368, 492, 523
Bsh1285I	CGRYCG	2 cut(s)	141, 252
BshFI	GGCC	4 cut(s)	68, 251, 279, 645
BshNI	GGYRCC	2 cut(s)	595, 666
BshTI	ACCGGT	1 cut(s)	929
BsiEI	CGRYCG	2 cut(s)	141, 252
BsiHKAI	GWGCWC	3 cut(s)	247, 358, 618
BsiHKCI	CYCGRG	2 cut(s)	240, 863
BsiSI	CCGG	9 cut(s)	65, 248, 287, 482, 554, 589, 744, 779, 930
BslI	CCNNNNNNNGG	3 cut(s)	286, 640, 964
BsnI	GGCC	4 cut(s)	68, 251, 279, 645
BsoBI	CYCGRG	2 cut(s)	240, 863
Bsp119I	TTCGAA	2 cut(s)	396, 563
Bsp120I	GGGCCC	1 cut(s)	643
Bsp1286I	GDGCHC	5 cut(s)	247, 358, 618, 647, 669
Bsp143I	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
Bsp19I	CCATGG	1 cut(s)	940
Bsp68I	TCGCGA	1 cut(s)	523
BspANI	GGCC	4 cut(s)	68, 251, 279, 645
BspCNI	CTCAG	2 cut(s)	76, 202
BspFNI	CGCG	3 cut(s)	368, 492, 523
BspHI	TCATGA	1 cut(s)	1217
BspLI	GGNNCC	5 cut(s)	285, 597, 644, 645, 668
BspMAI	CTGCAG	1 cut(s)	1069
BspMI	ACCTGC	2 cut(s)	583, 787
BspPI	GGATC	4 cut(s)	289, 304, 342, 730
BspT104I	TTCGAA	2 cut(s)	396, 563
BspT107I	GGYRCC	2 cut(s)	595, 666
BspTI	CTTAAG	1 cut(s)	1031
BsrBI	CCGCTC	3 cut(s)	467, 760, 810
BsrFI	RCCGGY	2 cut(s)	64, 929
BsrI	ACTGG	1 cut(s)	1145
BssAI	RCCGGY	2 cut(s)	64, 929
BssECI	CCNNGG	2 cut(s)	862, 940
BssMI	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
BssNI	GRCGYC	3 cut(s)	453, 606, 997
BssT1I	CCWWGG	1 cut(s)	940
Bst2UI	CCWGG	2 cut(s)	281, 457
Bst4CI	ACNGT	2 cut(s)	1007, 1092
Bst6I	CTCTTC	3 cut(s)	41, 47, 133
BstACI	GRCGYC	3 cut(s)	453, 606, 997
BstAFI	CTTAAG	1 cut(s)	1031
BstAPI	GCANNNNNTGC	1 cut(s)	1171
BstBAI	YACGTR	4 cut(s)	472, 504, 674, 1101
BstBI	TTCGAA	2 cut(s)	396, 563
BstC8I	GCNNGC	5 cut(s)	66, 272, 462, 525, 821
BstDEI	CTNAG	3 cut(s)	84, 210, 959
BstDSI	CCRYGG	1 cut(s)	940
BstF5I	GGATG	2 cut(s)	436, 631
BstFNI	CGCG	3 cut(s)	368, 492, 523
BstHHI	GCGC	6 cut(s)	209, 368, 494, 509, 796, 858
BstKTI	GATC	9 cut(s)	51, 297, 312, 337, 345, 683, 703, 713, 738
BstMBI	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
BstMCI	CGRYCG	2 cut(s)	141, 252
BstMWI	GCNNNNNNNGC	9 cut(s)	80, 251, 260, 276, 372, 409, 461, 816, 1171
BstNI	CCWGG	2 cut(s)	281, 457
BstNSI	RCATGY	1 cut(s)	1143
BstSCI	CCNGG	5 cut(s)	279, 285, 455, 552, 742
BstSFI	CTRYAG	3 cut(s)	883, 1065, 1074
BstSLI	GKGCMC	2 cut(s)	647, 669
BstUI	CGCG	3 cut(s)	368, 492, 523
BstV1I	GCAGC	6 cut(s)	191, 390, 1035, 1051, 1161, 1174
BstV2I	GAAGAC	3 cut(s)	142, 151, 1000
BstX2I	RGATCY	1 cut(s)	334
BstXI	CCANNNNNNTGG	1 cut(s)	223
BstYI	RGATCY	1 cut(s)	334
BstZI	CGGCCG	1 cut(s)	249
Bsu36I	CCTNAGG	1 cut(s)	959
BsuI	GTATCC	1 cut(s)	1129
BsuRI	GGCC	4 cut(s)	68, 251, 279, 645
BtgI	CCRYGG	1 cut(s)	940
BtgZI	GCGATG	1 cut(s)	97
BtrI	CACGTC	1 cut(s)	477
BtsCI	GGATG	2 cut(s)	436, 631
BtsI	GCAGTG	1 cut(s)	919
BtsIMutI	CAGTG	2 cut(s)	216, 919
BtuMI	TCGCGA	1 cut(s)	523
BveI	ACCTGC	2 cut(s)	583, 787
Cac8I	GCNNGC	5 cut(s)	66, 272, 462, 525, 821
CciI	TCATGA	1 cut(s)	1217
CfoI	GCGC	6 cut(s)	209, 368, 494, 509, 796, 858
Cfr10I	RCCGGY	2 cut(s)	64, 929
Cfr13I	GGNCC	4 cut(s)	283, 643, 644, 1081
CseI	GACGC	3 cut(s)	461, 614, 1005
Csp6I	GTAC	3 cut(s)	596, 763, 889
CspAI	ACCGGT	1 cut(s)	929
CviAII	CATG	6 cut(s)	18, 223, 941, 1140, 1166, 1218
CviQI	GTAC	3 cut(s)	596, 763, 889
DdeI	CTNAG	3 cut(s)	84, 210, 959
DpnI	GATC	9 cut(s)	50, 296, 311, 336, 344, 682, 702, 712, 737
DpnII	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
DraI	TTTAAA	1 cut(s)	549
DrdI	GACNNNNNNGTC	1 cut(s)	943
DseDI	GACNNNNNNGTC	1 cut(s)	943
EaeI	YGGCCR	2 cut(s)	66, 249
EagI	CGGCCG	1 cut(s)	249
Eam1104I	CTCTTC	3 cut(s)	41, 47, 133
EarI	CTCTTC	3 cut(s)	41, 47, 133
EciI	GGCGGA	1 cut(s)	313
Ecl136II	GAGCTC	2 cut(s)	245, 356
EclXI	CGGCCG	1 cut(s)	249
Eco130I	CCWWGG	1 cut(s)	940
Eco147I	AGGCCT	1 cut(s)	279
Eco24I	GRGCYC	3 cut(s)	247, 358, 647
Eco47I	GGWCC	2 cut(s)	283, 1081
Eco52I	CGGCCG	1 cut(s)	249
Eco53kI	GAGCTC	2 cut(s)	245, 356
Eco57I	CTGAAG	1 cut(s)	1073
Eco72I	CACGTG	3 cut(s)	472, 504, 1101
Eco81I	CCTNAGG	1 cut(s)	959
Eco88I	CYCGRG	2 cut(s)	240, 863
EcoICRI	GAGCTC	2 cut(s)	245, 356
EcoO109I	RGGNCCY	1 cut(s)	643
EcoRI	GAATTC	1 cut(s)	565
EcoRII	CCWGG	2 cut(s)	279, 455
EcoT14I	CCWWGG	1 cut(s)	940
EcoT38I	GRGCYC	3 cut(s)	247, 358, 647
ErhI	CCWWGG	1 cut(s)	940
FaeI	CATG	6 cut(s)	21, 226, 944, 1143, 1169, 1221
FatI	CATG	6 cut(s)	17, 222, 940, 1139, 1165, 1217
FblI	GTMKAC	1 cut(s)	318
FokI	GGATG	2 cut(s)	423, 638
FriOI	GRGCYC	3 cut(s)	247, 358, 647
FspBI	CTAG	1 cut(s)	837
GlaI	GCGC	6 cut(s)	208, 367, 493, 508, 795, 857
GsaI	CCCAGC	1 cut(s)	535
GsuI	CTGGAG	1 cut(s)	380
HaeIII	GGCC	4 cut(s)	68, 251, 279, 645
HapII	CCGG	9 cut(s)	65, 248, 287, 482, 554, 589, 744, 779, 930
HgaI	GACGC	3 cut(s)	461, 614, 1005
HhaI	GCGC	6 cut(s)	209, 368, 494, 509, 796, 858
Hin1I	GRCGYC	3 cut(s)	453, 606, 997
Hin1II	CATG	6 cut(s)	21, 226, 944, 1143, 1169, 1221
Hin6I	GCGC	6 cut(s)	207, 366, 492, 507, 794, 856
HinP1I	GCGC	6 cut(s)	207, 366, 492, 507, 794, 856
HinfI	GANTC	5 cut(s)	212, 227, 512, 560, 574
HpaII	CCGG	9 cut(s)	65, 248, 287, 482, 554, 589, 744, 779, 930
HphI	GGTGA	4 cut(s)	304, 799, 911, 982
Hpy166II	GTNNAC	3 cut(s)	216, 319, 765
Hpy188I	TCNGA	4 cut(s)	236, 579, 740, 1011
Hpy8I	GTNNAC	3 cut(s)	216, 319, 765
Hpy99I	CGWCG	3 cut(s)	455, 608, 724
HpyAV	CCTTC	6 cut(s)	175, 442, 704, 778, 871, 896
HpyCH4III	ACNGT	2 cut(s)	1007, 1092
HpyCH4IV	ACGT	7 cut(s)	471, 476, 503, 673, 767, 829, 1100
HpyCH4V	TGCA	2 cut(s)	447, 1067
HpyF10VI	GCNNNNNNNGC	9 cut(s)	80, 251, 260, 276, 372, 409, 461, 816, 1171
HpyF3I	CTNAG	3 cut(s)	84, 210, 959
HpySE526I	ACGT	7 cut(s)	471, 476, 503, 673, 767, 829, 1100
Hsp92I	GRCGYC	3 cut(s)	453, 606, 997
Hsp92II	CATG	6 cut(s)	21, 226, 944, 1143, 1169, 1221
HspAI	GCGC	6 cut(s)	207, 366, 492, 507, 794, 856
KpnI	GGTACC	1 cut(s)	599
KroI	GCCGGC	1 cut(s)	64
KroNI	GCCGGC	1 cut(s)	66
Kzo9I	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
LmnI	GCTCC	4 cut(s)	21, 250, 260, 353
Lsp1109I	GCAGC	6 cut(s)	191, 390, 1035, 1051, 1161, 1174
LweI	GCATC	1 cut(s)	456
MaeI	CTAG	1 cut(s)	837
MaeII	ACGT	7 cut(s)	471, 476, 503, 673, 767, 829, 1100
MaeIII	GTNAC	5 cut(s)	3, 472, 725, 970, 1001
MalI	GATC	9 cut(s)	50, 296, 311, 336, 344, 682, 702, 712, 737
MbiI	CCGCTC	3 cut(s)	467, 760, 810
MboI	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
MflI	RGATCY	1 cut(s)	334
MhlI	GDGCHC	5 cut(s)	247, 358, 618, 647, 669
MluCI	AATT	6 cut(s)	118, 565, 978, 1120, 1126, 1179
MlyI	GAGTC	3 cut(s)	221, 521, 583
MmeI	TCCRAC	3 cut(s)	763, 1002, 1074
MroNI	GCCGGC	1 cut(s)	64
MseI	TTAA	6 cut(s)	548, 770, 950, 1032, 1125, 1211
MslI	CAYNNNNRTG	1 cut(s)	221
MspA1I	CMGCKG	3 cut(s)	83, 204, 1064
MspCI	CTTAAG	1 cut(s)	1031
MspI	CCGG	9 cut(s)	65, 248, 287, 482, 554, 589, 744, 779, 930
MspR9I	CCNGG	5 cut(s)	281, 287, 457, 554, 744
MvaI	CCWGG	2 cut(s)	281, 457
MvnI	CGCG	3 cut(s)	368, 492, 523
MwoI	GCNNNNNNNGC	9 cut(s)	80, 251, 260, 276, 372, 409, 461, 816, 1171
NaeI	GCCGGC	1 cut(s)	66
NciI	CCSGG	3 cut(s)	287, 554, 744
NcoI	CCATGG	1 cut(s)	940
NdeII	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
NgoMIV	GCCGGC	1 cut(s)	64
NlaIII	CATG	6 cut(s)	21, 226, 944, 1143, 1169, 1221
NlaIV	GGNNCC	5 cut(s)	285, 597, 644, 645, 668
NmeAIII	GCCGAG	1 cut(s)	535
NmuCI	GTSAC	4 cut(s)	3, 472, 725, 970
NruI	TCGCGA	1 cut(s)	523
NspI	RCATGY	1 cut(s)	1143
NspV	TTCGAA	2 cut(s)	396, 563
PaeR7I	CTCGAG	1 cut(s)	240
PagI	TCATGA	1 cut(s)	1217
PceI	AGGCCT	1 cut(s)	279
PciI	ACATGT	1 cut(s)	1139
PcsI	WCGNNNNNNNCGW	2 cut(s)	468, 575
PdiI	GCCGGC	1 cut(s)	66
PfeI	GAWTC	2 cut(s)	227, 560
PinAI	ACCGGT	1 cut(s)	929
PleI	GAGTC	3 cut(s)	220, 520, 582
PmaCI	CACGTG	3 cut(s)	472, 504, 1101
PmlI	CACGTG	3 cut(s)	472, 504, 1101
PpsI	GAGTC	3 cut(s)	220, 520, 582
Ppu21I	YACGTR	4 cut(s)	472, 504, 674, 1101
PscI	ACATGT	1 cut(s)	1139
Psp124BI	GAGCTC	2 cut(s)	247, 358
Psp6I	CCWGG	2 cut(s)	279, 455
PspCI	CACGTG	3 cut(s)	472, 504, 1101
PspFI	CCCAGC	1 cut(s)	531
PspGI	CCWGG	2 cut(s)	279, 455
PspN4I	GGNNCC	5 cut(s)	285, 597, 644, 645, 668
PspOMI	GGGCCC	1 cut(s)	643
PspPI	GGNCC	4 cut(s)	283, 643, 644, 1081
PspXI	VCTCGAGB	1 cut(s)	240
PstI	CTGCAG	1 cut(s)	1069
PsuI	RGATCY	1 cut(s)	334
PvuII	CAGCTG	1 cut(s)	1064
RruI	TCGCGA	1 cut(s)	523
RsaI	GTAC	3 cut(s)	597, 764, 890
RsaNI	GTAC	3 cut(s)	596, 763, 889
RseI	CAYNNNNRTG	1 cut(s)	221
SacI	GAGCTC	2 cut(s)	247, 358
SaqAI	TTAA	6 cut(s)	548, 770, 950, 1032, 1125, 1211
Sau3AI	GATC	9 cut(s)	48, 294, 309, 334, 342, 680, 700, 710, 735
Sau96I	GGNCC	4 cut(s)	283, 643, 644, 1081
SchI	GAGTC	3 cut(s)	221, 521, 583
ScrFI	CCNGG	5 cut(s)	281, 287, 457, 554, 744
SduI	GDGCHC	5 cut(s)	247, 358, 618, 647, 669
SfaNI	GCATC	1 cut(s)	456
SfcI	CTRYAG	3 cut(s)	883, 1065, 1074
Sfr274I	CTCGAG	1 cut(s)	240
SfuI	TTCGAA	2 cut(s)	396, 563
SinI	GGWCC	2 cut(s)	283, 1081
SlaI	CTCGAG	1 cut(s)	240
SmiMI	CAYNNNNRTG	1 cut(s)	221
SmlI	CTYRAG	3 cut(s)	240, 376, 1031
SmoI	CTYRAG	3 cut(s)	240, 376, 1031
Sse9I	AATT	6 cut(s)	118, 565, 978, 1120, 1126, 1179
SseBI	AGGCCT	1 cut(s)	279
SspMI	CTAG	1 cut(s)	837
SstI	GAGCTC	2 cut(s)	247, 358
StuI	AGGCCT	1 cut(s)	279
StyD4I	CCNGG	5 cut(s)	279, 285, 455, 552, 742
StyI	CCWWGG	1 cut(s)	940
TaaI	ACNGT	2 cut(s)	1007, 1092
TaiI	ACGT	7 cut(s)	474, 479, 506, 676, 770, 832, 1103
TasI	AATT	6 cut(s)	118, 565, 978, 1120, 1126, 1179
TauI	GCSGC	7 cut(s)	204, 254, 467, 492, 813, 861, 897
TfiI	GAWTC	2 cut(s)	227, 560
Tru1I	TTAA	6 cut(s)	548, 770, 950, 1032, 1125, 1211
Tru9I	TTAA	6 cut(s)	548, 770, 950, 1032, 1125, 1211
TscAI	CASTG	2 cut(s)	223, 919
TseFI	GTSAC	4 cut(s)	3, 472, 725, 970
TseI	GCWGC	6 cut(s)	204, 403, 1048, 1064, 1162, 1174
Tsp45I	GTSAC	4 cut(s)	3, 472, 725, 970
TspDTI	ATGAA	1 cut(s)	642
TspGWI	ACGGA	2 cut(s)	220, 989
TspRI	CASTG	2 cut(s)	223, 919
Vha464I	CTTAAG	1 cut(s)	1031
VpaK11BI	GGWCC	2 cut(s)	283, 1081
XapI	RAATTY	3 cut(s)	565, 978, 1120
XceI	RCATGY	1 cut(s)	1143
XhoI	CTCGAG	1 cut(s)	240
XmiI	GTMKAC	1 cut(s)	318
XspI	CTAG	1 cut(s)	837

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.