Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011814

Physical Location & Seq

Genomic Coordinates Forward (+)

40411131 .. 40413060

Gene Structure 1930 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1344900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 891 bp

ATGTCGTCCGCCGATTTACAGCTTCCACCGGGGTTCAGGTTCCACCCGACGGACGAGGAGCTGGTGAGACACTACCTCTGCCGGAAATGCGCGTCGCAGCCGATATCAGTTCCGATCATTGCCGAAATCGATCTCTACAAATACGACCCCTGGGAGCTCCCAGGCTTGGCGTTGTACGGGGAAAAGGAGTGGTATTTCTTTTCGCCAAGGGATCGGAAGTACCCGAACGGTTCGAGGCCGAACCGGGCGGCCGGAAGCGGCTACTGGAAGGCGACCGGGGCCGATAAGCCGATTGGGCACCCGAAACCTGTGGGGATTAAGAAAGCTCTGGTGTTCTATGCTGGAAAAGCCCCTCGCGGCGAAAAGACCAACTGGATTATGCACGAATACCGCCTCGCCGACGTTGACCGCTCGCCGAGGAAGAAGAACAGCCTCAGGCTGGATGATTGGGTTCTGTGCCGAATATACAACAAGAAGGGGACCGTGGAGAACCAGCAGCCTCAGCAGCAGCAAGAACAACAGCGAACGACTAGTCGTAACGCGAGCAGTGGCTCGGAGTTCGAGGACAGGAAGCCGGATAATCTGAGGTGCCCGCCGCCGCCGGCTTCCGTGGTCCCCAGTCGTCACGTGAGCGATTACGTGTACTTCGACAACTCTGACTCGGTGCCGAAGCTGCACACGGACTCGAGCTGCTCGGAGCAGGTGGTGTCGCCGGAGTTGGCGTGCGAGGTGCAGAGCGAGCCCAAGTGGAAGGAGTGGGAGAAGGCGCTGGAAATGCCGTACAGTTACATGGATACGACGGTGGAGAACGGGTTCGGAGCGCAGTTCCAGAGCGGGAATGAGATGTCGCCGCTGCAGGATATCTTTATGTACCTGCAGAAGCCGTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002831 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009611 GO:0009628 GO:0009787 GO:0009788 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009968 GO:0010154 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0032101 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043900 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0047484 GO:0048316 GO:0048364 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0099402 GO:0140110 GO:1900055 GO:1900057 GO:1900150 GO:1901000 GO:1901002 GO:1901363 GO:1901419 GO:1901420 GO:1902584 GO:1902680 GO:1903506 GO:1903508 GO:1905623 GO:1905957 GO:1905958 GO:2000024 GO:2000026 GO:2000070 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

297

Amino Acids

33.99

Weight (kDa)

7.0

Isoelectric Point (pI)

57.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	9 - 132	1.3e-49	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01720 AT1G77450
fragaria_vesca	FvH4_2g27430
malus_domestica	MD00G1028600.v1.1 MD15G1344900.v1.1
prunus_persica	Prupe.1G493100_v2.0.a1
pyrus_communis	pycom15g30950
rosa_chinensis	RchiOBHm_Chr6g0296281
rosa_laevigata	RLG00000011710
rosa_multiflora	Rmu_sc0004708.1_g000008 Rmu_sc0007700.1_g000001
rosa_roxburghii	Rroxscaffold_7G00171670 Rroxscaffold_7G00178400
rosa_rugosa	Rorug06G0260600
rosa_samantha	Rh6AG372300 Rh6BG380300 Rh6CG385700 Rh6DG373000
rosa_wichuraiana	Rw6G032460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	691
Acc36I	ACCTGC	2 cut(s)	691, 882
AccB1I	GGYRCC	3 cut(s)	297, 588, 664
AccBSI	CCGCTC	2 cut(s)	411, 834
AccII	CGCG	3 cut(s)	92, 357, 542
AclWI	GGATC	1 cut(s)	219
AcoI	YGGCCR	1 cut(s)	249
AcvI	CACGTG	1 cut(s)	628
AfaI	GTAC	6 cut(s)	176, 221, 644, 782, 872, 887
AfiI	CCNNNNNNNGG	3 cut(s)	49, 166, 439
AflIII	ACRYGT	1 cut(s)	639
AhlI	ACTAGT	1 cut(s)	530
AjnI	CCWGG	2 cut(s)	149, 160
AloI	GAACNNNNNNTCC	2 cut(s)	797, 829
AluBI	AGCT	6 cut(s)	22, 61, 157, 326, 673, 690
AluI	AGCT	6 cut(s)	22, 61, 157, 326, 673, 690
Alw21I	GWGCWC	1 cut(s)	159
Alw26I	GTCTC	1 cut(s)	61
AlwI	GGATC	1 cut(s)	219
Ama87I	CYCGRG	1 cut(s)	685
AoxI	GGCC	3 cut(s)	236, 249, 279
ApeKI	GCWGC	7 cut(s)	97, 496, 505, 508, 673, 690, 853
ArsI	GACNNNNNNTTYG	2 cut(s)	517, 549
Asp700I	GAANNNNTTC	1 cut(s)	812
AspLEI	GCGC	3 cut(s)	92, 769, 823
AspS9I	GGNCC	3 cut(s)	279, 480, 613
AsuC2I	CCSGG	3 cut(s)	30, 245, 277
AsuHPI	GGTGA	1 cut(s)	76
AvaI	CYCGRG	1 cut(s)	685
AvaII	GGWCC	2 cut(s)	480, 613
AxyI	CCTNAGG	1 cut(s)	434
BaeGI	GKGCMC	2 cut(s)	300, 593
BaeI	ACNNNNGTAYC	2 cut(s)	786, 819
BanI	GGYRCC	3 cut(s)	297, 588, 664
BanII	GRGCYC	2 cut(s)	159, 744
BbrPI	CACGTG	1 cut(s)	628
Bbv12I	GWGCWC	1 cut(s)	159
BbvCI	CCTCAGC	1 cut(s)	501
BbvI	GCAGC	7 cut(s)	109, 508, 517, 520, 660, 677, 840
BceAI	ACGGC	2 cut(s)	763, 868
BciT130I	CCWGG	2 cut(s)	151, 162
BciVI	GTATCC	1 cut(s)	787
BcnI	CCSGG	3 cut(s)	30, 245, 277
BcoDI	GTCTC	1 cut(s)	61
BcuI	ACTAGT	1 cut(s)	530
BfaI	CTAG	1 cut(s)	531
BfmI	CTRYAG	2 cut(s)	854, 875
BfoI	RGCGCY	1 cut(s)	770
BfuAI	ACCTGC	2 cut(s)	691, 882
BfuI	GTATCC	1 cut(s)	787
BglI	GCCNNNNNGGC	1 cut(s)	295
Bme1390I	CCNGG	5 cut(s)	30, 151, 162, 245, 277
Bme18I	GGWCC	2 cut(s)	480, 613
BmeT110I	CYCGRG	1 cut(s)	685
BmgT120I	GGNCC	3 cut(s)	279, 480, 613
BmiI	GGNNCC	7 cut(s)	41, 280, 299, 481, 590, 615, 666
BmrFI	CCNGG	5 cut(s)	30, 151, 162, 245, 277
BmrI	ACTGGG	1 cut(s)	612
BmuI	ACTGGG	1 cut(s)	612
Bpu10I	CCTNAGC	1 cut(s)	501
BpuMI	CCSGG	3 cut(s)	30, 245, 277
Bsa29I	ATCGAT	1 cut(s)	129
BsaAI	YACGTR	2 cut(s)	628, 640
BsaJI	CCNNGG	9 cut(s)	29, 149, 150, 160, 206, 276, 416, 483, 609
BsaXI	ACNNNNNCTCC	4 cut(s)	707, 737, 797, 827
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 166, 439
Bse118I	RCCGGY	1 cut(s)	601
Bse1I	ACTGG	3 cut(s)	269, 377, 618
Bse21I	CCTNAGG	1 cut(s)	434
Bse3DI	GCAATG	1 cut(s)	117
BseBI	CCWGG	2 cut(s)	151, 162
BseCI	ATCGAT	1 cut(s)	129
BseDI	CCNNGG	9 cut(s)	29, 149, 150, 160, 206, 276, 416, 483, 609
BseGI	GGATG	1 cut(s)	448
BseLI	CCNNNNNNNGG	3 cut(s)	49, 166, 439
BseMI	GCAATG	1 cut(s)	117
BseMII	CTCAG	3 cut(s)	448, 515, 575
BseNI	ACTGG	3 cut(s)	269, 377, 618
BseRI	GAGGAG	1 cut(s)	71
BseSI	GKGCMC	2 cut(s)	300, 593
BseX3I	CGGCCG	1 cut(s)	249
BseXI	GCAGC	7 cut(s)	109, 508, 517, 520, 660, 677, 840
BsgI	GTGCAG	2 cut(s)	659, 752
Bsh1236I	CGCG	3 cut(s)	92, 357, 542
Bsh1285I	CGRYCG	2 cut(s)	252, 276
BshFI	GGCC	3 cut(s)	238, 251, 281
BshNI	GGYRCC	3 cut(s)	297, 588, 664
BshVI	ATCGAT	1 cut(s)	129
BsiEI	CGRYCG	2 cut(s)	252, 276
BsiHKAI	GWGCWC	1 cut(s)	159
BsiHKCI	CYCGRG	1 cut(s)	685
BsiSI	CCGG	8 cut(s)	29, 82, 244, 252, 276, 575, 602, 713
BslFI	GGGAC	2 cut(s)	493, 599
BslI	CCNNNNNNNGG	3 cut(s)	49, 166, 439
BsmAI	GTCTC	1 cut(s)	61
BsmFI	GGGAC	2 cut(s)	493, 599
BsnI	GGCC	3 cut(s)	238, 251, 281
BsoBI	CYCGRG	1 cut(s)	685
Bsp1286I	GDGCHC	4 cut(s)	159, 300, 593, 744
Bsp143I	GATC	3 cut(s)	114, 130, 211
BspANI	GGCC	3 cut(s)	238, 251, 281
BspCNI	CTCAG	3 cut(s)	447, 514, 576
BspDI	ATCGAT	1 cut(s)	129
BspFNI	CGCG	3 cut(s)	92, 357, 542
BspLI	GGNNCC	7 cut(s)	41, 280, 299, 481, 590, 615, 666
BspMAI	CTGCAG	2 cut(s)	858, 879
BspMI	ACCTGC	2 cut(s)	691, 882
BspPI	GGATC	1 cut(s)	219
BspT107I	GGYRCC	3 cut(s)	297, 588, 664
BsrBI	CCGCTC	2 cut(s)	411, 834
BsrDI	GCAATG	1 cut(s)	117
BsrFI	RCCGGY	1 cut(s)	601
BsrI	ACTGG	3 cut(s)	269, 377, 618
BssAI	RCCGGY	1 cut(s)	601
BssECI	CCNNGG	9 cut(s)	29, 149, 150, 160, 206, 276, 416, 483, 609
BssMI	GATC	3 cut(s)	114, 130, 211
BssT1I	CCWWGG	1 cut(s)	206
Bst2UI	CCWGG	2 cut(s)	151, 162
Bst4CI	ACNGT	4 cut(s)	230, 484, 785, 802
BstBAI	YACGTR	2 cut(s)	628, 640
BstC8I	GCNNGC	6 cut(s)	413, 544, 593, 603, 724, 740
BstDEI	CTNAG	3 cut(s)	434, 501, 584
BstDSI	CCRYGG	2 cut(s)	483, 609
BstF5I	GGATG	1 cut(s)	448
BstFNI	CGCG	3 cut(s)	92, 357, 542
BstH2I	RGCGCY	1 cut(s)	770
BstHHI	GCGC	3 cut(s)	92, 769, 823
BstKTI	GATC	3 cut(s)	117, 133, 214
BstMAI	GTCTC	1 cut(s)	61
BstMBI	GATC	3 cut(s)	114, 130, 211
BstMCI	CGRYCG	2 cut(s)	252, 276
BstMWI	GCNNNNNNNGC	9 cut(s)	87, 278, 295, 347, 502, 505, 673, 739, 775
BstNI	CCWGG	2 cut(s)	151, 162
BstSCI	CCNGG	5 cut(s)	28, 149, 160, 243, 275
BstSFI	CTRYAG	2 cut(s)	854, 875
BstSLI	GKGCMC	2 cut(s)	300, 593
BstUI	CGCG	3 cut(s)	92, 357, 542
BstV1I	GCAGC	7 cut(s)	109, 508, 517, 520, 660, 677, 840
BstZI	CGGCCG	1 cut(s)	249
Bsu15I	ATCGAT	1 cut(s)	129
Bsu36I	CCTNAGG	1 cut(s)	434
BsuI	GTATCC	1 cut(s)	787
BsuRI	GGCC	3 cut(s)	238, 251, 281
BsuTUI	ATCGAT	1 cut(s)	129
BtgI	CCRYGG	2 cut(s)	483, 609
BtsCI	GGATG	1 cut(s)	448
BtsI	GCAGTG	1 cut(s)	553
BtsIMutI	CAGTG	1 cut(s)	553
BveI	ACCTGC	2 cut(s)	691, 882
Cac8I	GCNNGC	6 cut(s)	413, 544, 593, 603, 724, 740
CfoI	GCGC	3 cut(s)	92, 769, 823
Cfr10I	RCCGGY	1 cut(s)	601
Cfr13I	GGNCC	3 cut(s)	279, 480, 613
ClaI	ATCGAT	1 cut(s)	129
CseI	GACGC	1 cut(s)	81
Csp6I	GTAC	6 cut(s)	175, 220, 643, 781, 871, 886
CviAII	CATG	1 cut(s)	790
CviQI	GTAC	6 cut(s)	175, 220, 643, 781, 871, 886
DdeI	CTNAG	3 cut(s)	434, 501, 584
DpnI	GATC	3 cut(s)	116, 132, 213
DpnII	GATC	3 cut(s)	114, 130, 211
EaeI	YGGCCR	1 cut(s)	249
EagI	CGGCCG	1 cut(s)	249
Ecl136II	GAGCTC	1 cut(s)	157
EclXI	CGGCCG	1 cut(s)	249
Eco130I	CCWWGG	1 cut(s)	206
Eco24I	GRGCYC	2 cut(s)	159, 744
Eco32I	GATATC	2 cut(s)	105, 862
Eco47I	GGWCC	2 cut(s)	480, 613
Eco52I	CGGCCG	1 cut(s)	249
Eco53kI	GAGCTC	1 cut(s)	157
Eco72I	CACGTG	1 cut(s)	628
Eco81I	CCTNAGG	1 cut(s)	434
Eco88I	CYCGRG	1 cut(s)	685
EcoICRI	GAGCTC	1 cut(s)	157
EcoRII	CCWGG	2 cut(s)	149, 160
EcoRV	GATATC	2 cut(s)	105, 862
EcoT14I	CCWWGG	1 cut(s)	206
EcoT38I	GRGCYC	2 cut(s)	159, 744
ErhI	CCWWGG	1 cut(s)	206
FaeI	CATG	1 cut(s)	793
FaiI	YATR	5 cut(s)	339, 380, 466, 791, 869
FaqI	GGGAC	2 cut(s)	493, 599
FatI	CATG	1 cut(s)	789
FauI	CCCGC	2 cut(s)	600, 827
FokI	GGATG	1 cut(s)	455
FriOI	GRGCYC	2 cut(s)	159, 744
FspBI	CTAG	1 cut(s)	531
GlaI	GCGC	3 cut(s)	91, 768, 822
HaeII	RGCGCY	1 cut(s)	770
HaeIII	GGCC	3 cut(s)	238, 251, 281
HapII	CCGG	8 cut(s)	29, 82, 244, 252, 276, 575, 602, 713
HgaI	GACGC	1 cut(s)	81
HhaI	GCGC	3 cut(s)	92, 769, 823
Hin1II	CATG	1 cut(s)	793
Hin6I	GCGC	3 cut(s)	90, 767, 821
HinP1I	GCGC	3 cut(s)	90, 767, 821
HincII	GTYRAC	1 cut(s)	406
HindII	GTYRAC	1 cut(s)	406
HinfI	GANTC	2 cut(s)	659, 683
HpaII	CCGG	8 cut(s)	29, 82, 244, 252, 276, 575, 602, 713
HphI	GGTGA	1 cut(s)	76
Hpy166II	GTNNAC	2 cut(s)	406, 643
Hpy188I	TCNGA	7 cut(s)	114, 216, 556, 585, 658, 697, 818
Hpy188III	TCNNGA	1 cut(s)	829
Hpy8I	GTNNAC	2 cut(s)	406, 643
Hpy99I	CGWCG	4 cut(s)	52, 97, 404, 802
HpyAV	CCTTC	4 cut(s)	262, 469, 745, 757
HpyCH4III	ACNGT	4 cut(s)	230, 484, 785, 802
HpyCH4IV	ACGT	3 cut(s)	402, 627, 639
HpyCH4V	TGCA	5 cut(s)	382, 676, 733, 856, 877
HpyF10VI	GCNNNNNNNGC	9 cut(s)	87, 278, 295, 347, 502, 505, 673, 739, 775
HpyF3I	CTNAG	3 cut(s)	434, 501, 584
HpySE526I	ACGT	3 cut(s)	402, 627, 639
Hsp92II	CATG	1 cut(s)	793
HspAI	GCGC	3 cut(s)	90, 767, 821
KroI	GCCGGC	1 cut(s)	601
KroNI	GCCGGC	1 cut(s)	603
Kzo9I	GATC	3 cut(s)	114, 130, 211
LmnI	GCTCC	5 cut(s)	58, 154, 162, 697, 818
Lsp1109I	GCAGC	7 cut(s)	109, 508, 517, 520, 660, 677, 840
MaeI	CTAG	1 cut(s)	531
MaeII	ACGT	3 cut(s)	402, 627, 639
MaeIII	GTNAC	3 cut(s)	536, 623, 785
MalI	GATC	3 cut(s)	116, 132, 213
MbiI	CCGCTC	2 cut(s)	411, 834
MboI	GATC	3 cut(s)	114, 130, 211
MboII	GAAGA	2 cut(s)	433, 436
MhlI	GDGCHC	4 cut(s)	159, 300, 593, 744
MlyI	GAGTC	2 cut(s)	653, 677
MroNI	GCCGGC	1 cut(s)	601
MroXI	GAANNNNTTC	1 cut(s)	812
MseI	TTAA	1 cut(s)	318
MspA1I	CMGCKG	1 cut(s)	853
MspI	CCGG	8 cut(s)	29, 82, 244, 252, 276, 575, 602, 713
MspR9I	CCNGG	5 cut(s)	30, 151, 162, 245, 277
MvaI	CCWGG	2 cut(s)	151, 162
MvnI	CGCG	3 cut(s)	92, 357, 542
MwoI	GCNNNNNNNGC	9 cut(s)	87, 278, 295, 347, 502, 505, 673, 739, 775
NaeI	GCCGGC	1 cut(s)	603
NciI	CCSGG	3 cut(s)	30, 245, 277
NdeII	GATC	3 cut(s)	114, 130, 211
NgoMIV	GCCGGC	1 cut(s)	601
NlaIII	CATG	1 cut(s)	793
NlaIV	GGNNCC	7 cut(s)	41, 280, 299, 481, 590, 615, 666
NmeAIII	GCCGAG	1 cut(s)	441
NmuCI	GTSAC	1 cut(s)	623
PaeR7I	CTCGAG	1 cut(s)	685
PaqCI	CACCTGC	1 cut(s)	691
PasI	CCCWGGG	1 cut(s)	150
PcsI	WCGNNNNNNNCGW	1 cut(s)	645
PdiI	GCCGGC	1 cut(s)	603
PdmI	GAANNNNTTC	1 cut(s)	812
PleI	GAGTC	2 cut(s)	653, 677
PmaCI	CACGTG	1 cut(s)	628
PmlI	CACGTG	1 cut(s)	628
PpsI	GAGTC	2 cut(s)	653, 677
Ppu21I	YACGTR	2 cut(s)	628, 640
Psp124BI	GAGCTC	1 cut(s)	159
Psp6I	CCWGG	2 cut(s)	149, 160
PspCI	CACGTG	1 cut(s)	628
PspGI	CCWGG	2 cut(s)	149, 160
PspN4I	GGNNCC	7 cut(s)	41, 280, 299, 481, 590, 615, 666
PspPI	GGNCC	3 cut(s)	279, 480, 613
PspXI	VCTCGAGB	1 cut(s)	685
PstI	CTGCAG	2 cut(s)	858, 879
RsaI	GTAC	6 cut(s)	176, 221, 644, 782, 872, 887
RsaNI	GTAC	6 cut(s)	175, 220, 643, 781, 871, 886
SacI	GAGCTC	1 cut(s)	159
SaqAI	TTAA	1 cut(s)	318
Sau3AI	GATC	3 cut(s)	114, 130, 211
Sau96I	GGNCC	3 cut(s)	279, 480, 613
SchI	GAGTC	2 cut(s)	653, 677
ScrFI	CCNGG	5 cut(s)	30, 151, 162, 245, 277
SduI	GDGCHC	4 cut(s)	159, 300, 593, 744
SfcI	CTRYAG	2 cut(s)	854, 875
Sfr274I	CTCGAG	1 cut(s)	685
SinI	GGWCC	2 cut(s)	480, 613
SlaI	CTCGAG	1 cut(s)	685
SmlI	CTYRAG	1 cut(s)	685
SmoI	CTYRAG	1 cut(s)	685
SpeI	ACTAGT	1 cut(s)	530
SspMI	CTAG	1 cut(s)	531
SstI	GAGCTC	1 cut(s)	159
StyD4I	CCNGG	5 cut(s)	28, 149, 160, 243, 275
StyI	CCWWGG	1 cut(s)	206
TaaI	ACNGT	4 cut(s)	230, 484, 785, 802
TaiI	ACGT	3 cut(s)	405, 630, 642
TaqI	TCGA	5 cut(s)	129, 233, 561, 648, 686
TatI	WGTACW	1 cut(s)	642
TauI	GCSGC	6 cut(s)	251, 261, 360, 598, 601, 853
Tru1I	TTAA	1 cut(s)	318
Tru9I	TTAA	1 cut(s)	318
TscAI	CASTG	1 cut(s)	553
TseFI	GTSAC	1 cut(s)	623
TseI	GCWGC	7 cut(s)	97, 496, 505, 508, 673, 690, 853
Tsp45I	GTSAC	1 cut(s)	623
TspGWI	ACGGA	3 cut(s)	65, 598, 695
TspRI	CASTG	1 cut(s)	553
VpaK11BI	GGWCC	2 cut(s)	480, 613
XhoI	CTCGAG	1 cut(s)	685
XmnI	GAANNNNTTC	1 cut(s)	812
XspI	CTAG	1 cut(s)	531

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.