MD15G1376600.v1.1

FAM91 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004069
Physical Location & Seq
Forward (+)
46070556 .. 46073689
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1376600.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCAGCGTGCTCCGGCGACGATCGAGAAACAGTTGTTACTGAAAGCAATCAAAGAAGAGTGCCCGTGGGAGAGTCTCCCGAAACGCCTTCAAGTGGCATTGTCTTCCAAAGAAGAATGGCACAGAAGGGTAATTGAGCATTGCGTCAAGAAAAGACTTCCATGGAACACTTGCTTTGCTCGGCAAGTGTGCAAAGAAAGTGAATATTATGAAGACATGATGCGGTACTTACGAAGGAATTTAGCACTATTTCCCTATCACCTTGCGGAATATGTTTGCCGCGTAATGAGGGTCTCCCCATTCAGATATTACTGCGATATGATTTTTGAAGTTATGAGAAATGAACAACCTTATGACAGCATCCCCAATTTTAGTGCAGCAGATGCCTTGCGCCTTACAGGGATAGGGAGAAATGAATTTATTGATATAATGAACAAGTGCAGATCTAAGAAAATCATGTGGAAGCTGAACAAGTCAATTGCAAAAGAACTTTTACCTACACAACCTGTGGATTTTACAATTGAACCGTGGTGGGGAATTTGTCTTGTGAATTTCACGCTGGAGGAATTTAAGGTGAGAACTGAGACTAAAATCACTTTGAGCTCTGAACAGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM91_C FAM91_N

Protein Analysis

206

Amino Acids

24.54

Weight (kDa)

8.93

Isoelectric Point (pI)

59.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM91_N PF14647 17 - 199 6.9e-61 FAM91 N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AciI CCGC 3 cut(s) 223, 266, 280
AcsI RAATTY 5 cut(s) 238, 416, 537, 550, 566
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 94, 533
AgsI TTSAA 3 cut(s) 92, 329, 524
AluBI AGCT 2 cut(s) 466, 603
AluI AGCT 2 cut(s) 466, 603
Alw21I GWGCWC 2 cut(s) 13, 605
Alw26I GTCTC 3 cut(s) 80, 298, 578
ApeKI GCWGC 2 cut(s) 4, 377
ApoI RAATTY 5 cut(s) 238, 416, 537, 550, 566
AspLEI GCGC 1 cut(s) 393
AsuHPI GGTGA 2 cut(s) 251, 586
BaeGI GKGCMC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 605
BbsI GAAGAC 2 cut(s) 96, 219
Bbv12I GWGCWC 2 cut(s) 13, 605
BbvI GCAGC 2 cut(s) 16, 389
BcoDI GTCTC 3 cut(s) 80, 298, 578
BglII AGATCT 1 cut(s) 443
BisI GCNGC 3 cut(s) 5, 280, 378
BlsI GCNGC 3 cut(s) 6, 281, 379
BmsI GCATC 3 cut(s) 210, 369, 373
BpiI GAAGAC 2 cut(s) 96, 219
BpmI CTGGAG 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 3 cut(s) 65, 161, 527
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 533
Bse3DI GCAATG 1 cut(s) 139
BseDI CCNNGG 3 cut(s) 65, 161, 527
BseGI GGATG 1 cut(s) 360
BseLI CCNNNNNNNGG 2 cut(s) 94, 533
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 573
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 2 cut(s) 16, 389
BsgI GTGCAG 2 cut(s) 396, 460
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 24
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 13, 605
BsiSI CCGG 1 cut(s) 14
BslI CCNNNNNNNGG 2 cut(s) 94, 533
BsmAI GTCTC 3 cut(s) 80, 298, 578
Bso31I GGTCTC 1 cut(s) 298
Bsp1286I GDGCHC 3 cut(s) 13, 65, 605
Bsp143I GATC 2 cut(s) 21, 443
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 3 cut(s) 223, 266, 280
BspCNI CTCAG 1 cut(s) 574
BspFNI CGCG 1 cut(s) 282
BspTNI GGTCTC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 139
BssECI CCNNGG 3 cut(s) 65, 161, 527
BssMI GATC 2 cut(s) 21, 443
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 33, 528, 612
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 2 cut(s) 447, 582
BstDSI CCRYGG 3 cut(s) 65, 161, 527
BstF5I GGATG 1 cut(s) 360
BstFNI CGCG 1 cut(s) 282
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 2 cut(s) 24, 446
BstMAI GTCTC 3 cut(s) 80, 298, 578
BstMBI GATC 2 cut(s) 21, 443
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstSLI GKGCMC 1 cut(s) 65
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 16, 389
BstV2I GAAGAC 2 cut(s) 96, 219
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
BtgI CCRYGG 3 cut(s) 65, 161, 527
BtsCI GGATG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 617
Cac8I GCNNGC 1 cut(s) 9
CfoI GCGC 1 cut(s) 393
CseI GACGC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 226
CviAII CATG 3 cut(s) 162, 217, 457
CviJI RGCY 2 cut(s) 466, 603
CviKI_1 RGCY 2 cut(s) 466, 603
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 2 cut(s) 447, 582
DpnI GATC 2 cut(s) 23, 445
DpnII GATC 2 cut(s) 21, 443
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 605
Eco31I GGTCTC 1 cut(s) 298
Eco53kI GAGCTC 1 cut(s) 603
EcoICRI GAGCTC 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 605
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 3 cut(s) 165, 220, 460
FaiI YATR 9 cut(s) 163, 210, 218, 273, 320, 335, 354, 428, 458
FatI CATG 3 cut(s) 161, 216, 456
Fnu4HI GCNGC 3 cut(s) 5, 280, 378
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 605
Fsp4HI GCNGC 3 cut(s) 5, 280, 378
GlaI GCGC 1 cut(s) 392
GluI GCNGC 3 cut(s) 5, 280, 378
GsuI CTGGAG 1 cut(s) 581
HapII CCGG 1 cut(s) 14
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 393
Hin1II CATG 3 cut(s) 165, 220, 460
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HinfI GANTC 1 cut(s) 73
HpaII CCGG 1 cut(s) 14
HphI GGTGA 2 cut(s) 251, 586
Hpy188I TCNGA 2 cut(s) 305, 607
Hpy188III TCNNGA 3 cut(s) 25, 79, 148
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 3 cut(s) 98, 120, 228
HpyCH4III ACNGT 3 cut(s) 33, 528, 612
HpyCH4V TGCA 5 cut(s) 4, 192, 377, 441, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 2 cut(s) 447, 582
Hsp92II CATG 3 cut(s) 165, 220, 460
HspAI GCGC 1 cut(s) 391
Kzo9I GATC 2 cut(s) 21, 443
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 4 cut(s) 27, 384, 519, 545
Lsp1109I GCAGC 2 cut(s) 16, 389
LweI GCATC 3 cut(s) 210, 369, 373
MaeIII GTNAC 1 cut(s) 36
MalI GATC 2 cut(s) 23, 445
MboI GATC 2 cut(s) 21, 443
MboII GAAGA 4 cut(s) 68, 96, 125, 224
MfeI CAATTG 2 cut(s) 477, 519
MflI RGATCY 1 cut(s) 443
MhlI GDGCHC 3 cut(s) 13, 65, 605
MluCI AATT 9 cut(s) 132, 238, 367, 416, 477, 519, 537, 550, 566
MlyI GAGTC 1 cut(s) 82
MnlI CCTC 2 cut(s) 282, 556
MseI TTAA 1 cut(s) 570
MspI CCGG 1 cut(s) 14
MunI CAATTG 2 cut(s) 477, 519
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 383
NcoI CCATGG 1 cut(s) 161
NdeII GATC 2 cut(s) 21, 443
NlaIII CATG 3 cut(s) 165, 220, 460
NmeAIII GCCGAG 1 cut(s) 160
PkrI GCNGC 3 cut(s) 6, 281, 379
Ple19I CGATCG 1 cut(s) 24
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
Psp124BI GAGCTC 1 cut(s) 605
PsuI RGATCY 1 cut(s) 443
PvuI CGATCG 1 cut(s) 24
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SacI GAGCTC 1 cut(s) 605
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 3 cut(s) 5, 280, 378
Sau3AI GATC 2 cut(s) 21, 443
SchI GAGTC 1 cut(s) 82
SduI GDGCHC 3 cut(s) 13, 65, 605
SetI ASST 7 cut(s) 264, 352, 468, 499, 508, 576, 605
SfaNI GCATC 3 cut(s) 210, 369, 373
Sse9I AATT 9 cut(s) 132, 238, 367, 416, 477, 519, 537, 550, 566
SsiI CCGC 3 cut(s) 223, 266, 280
SspI AATATT 1 cut(s) 206
SstI GAGCTC 1 cut(s) 605
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 3 cut(s) 33, 528, 612
TaqI TCGA 1 cut(s) 24
TasI AATT 9 cut(s) 132, 238, 367, 416, 477, 519, 537, 550, 566
TauI GCSGC 1 cut(s) 282
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 1 cut(s) 617
TseI GCWGC 2 cut(s) 4, 377
TspDTI ATGAA 4 cut(s) 225, 357, 429, 446
TspRI CASTG 1 cut(s) 617
XapI RAATTY 5 cut(s) 238, 416, 537, 550, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.