Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004069

Physical Location & Seq

Genomic Coordinates Forward (+)

46073691 .. 46079672

Gene Structure 5982 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1376700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGGCAACAATAGATAAAATATGTAAAGAGGAAGCAAATTTATATATTTTATTCAATTCTGATATTGTTAAAGGTCTTCACCAACGAGGATTAGTATACTTTGATGTCCCTGTGTATCCTGATGACCGTTTTAAAGTTTCCAGGCTCGAAGGATTTGTTTCAAACAAGGAGCAATCTTATGAAGATCCTATAGAGGAGTTACTATATGCAGTTTTTGTCGTTTCAAGCGAGAATGCAACTGTTGCTGAACTGGCAACAACATTACAGGCTGACCTTGCACAGCTGCGGGCTGCTGCATCCTTTGCATGTCAATTGGGATGGGCGGTAAAAGTATTAGATCCAGCATCTGTGCTTCGAGATACAAGTTTACCTGGATCTCCTAGAAACAGTCTCCATGAAGAAGATATTTCTTGTCGTAGTACAAGCTCAGCAAATATGTTTGCCAATGGTGAAGCTAGTCTACAAGGAGATGTTTCAGGGACAGGAAACTCTTTGCATGATCGTGTTGCATTTGTTGTTGATGCTAACATAACATCCTACCTTATGATGGGGTCTGTCTCACCGGGCTTGAAATCGCATGCTGTGACATTGTATGAAGCAGGAAAGTTGGGTCATGGTAGTATTACCGATCTCTGTAAGGATCTGAGTACGCTAGAGGGAACAAAATTTGAAGGAGAACTACAGGAATTTGCAAATCATGCATTTAGCCTCCGTTGTGTTTTGGAATGCCTTCAATCAGGAGGAGTTGCAACTGATGTGAAAACAGAAGAAGTTTGCAAGAACATGGATATGATGACATCAAACAATGACGAGGCCACACTCATAGCTGATGCCACCTTGACTAACAAATCAGAACACTTAGCAATACATGAAGTTGGGGTCGAGGATGATATTTCTGTGAAATCAGGGATACCTGAGGAAGGTTCCGTTTTGGCTGAACCTGTTAGTGATGGAGGAAGTGATGGAACATTAATTGGTACACCATCAGAAGATATCACCAGCTTAACTGAAGTCCCTAAACCAGACTTGAATCTCCAGAATAATGAGAAATCGATGCATGCTGAAGGGTTAGACGTTGAAAGAGAAATGTTAAAGAAGAAAAAGAAATTCCGAGTGGATATCCTTCGCTGTGAAAGCTTGGCGTCTCTTGCACCAGAAGCTTTAGATCGGTTGTTTTGTCGTGACTATGATATTGTTGTATCTATTATTCCTCTTCCACCTTCGTCGGTACTTCCTGGACCAGCAGGTCCTATTAATTTTGGGCCTCCCTCGTATTCATGCATGACACCTTGGATGAAACTAGTATTGTACTCAACTGTGGCATGTGGACCCCTATCTGTTATTCTGATGAAAGGACAATGTTTACGATTGCTTCCTGCCCCGCTGGCCGGTTGTGAGAAAGCCCTTTTGTGGTCTTGGGATGGTTCTACAATTGGAGGGTTGGGGGGCAAGTTCGAAAGAAGTCTAGTAAATGGAAATGTACTTTTACATTGTTTAAATTCACTTCTGAAATATTCTGCTGTACTTGTGCAGCCCCTCAGTAGATATGACCTCGACAAATCTGGAAGAATCATCACCATGGATATTCCATTACCCTTAAAGAACTATGACGGTTCAGTTGGTTGCATAGGGGAGGAATTGGAACTGAGTGAGAAGGAAAGGTCAAAACTGAAATCTCTCTTAGGAAATATGGGAAACAAGATAGAGTTGTGGACAGTTGGCTATATTCGCTTATTGAAACTTTTTAAAGAAAGAGATTCAGAACACTTTGCACCTGATGATGAGAAGTATGAATGGGTCCCGTTGAGCGTCGAATTTGGAATGCCATTGTTTAGTCCAAAATTGTGTAATAACATATGTAAAAGATTGGTCTCGTCGCAATTACTTGAAAAAGATTTACTCACTGAGCATCATGATGCAATGCAAAGCCTAAGGAAAAGGTTGCGTGATGTTTGTGCAGAGTACCAAGCAACAGGTTCTGCTGCTAAACTTCTTTACCAAAAAGAGCAATCAAAGGACTTCTCACGACATCTCATGAATTATGCTAGTGGAAGGTGGAATCCACTTGTCGACCCATCCTCTCCCATTTCAGGAGCCTCGAGTGAGCAACAGAGACTAAAACTTGCTAACCGGCATCGTTCCCAAACTGAAGTCTTGAGCTTTGATGGAAGCATTTTAAGATCATACGCTCTAGCTTCTGTTTACGAGGCTGCCACAAGGCCAGTTGAAGGAGCTCCTCCGGTGAGCACTACAAAAGTTGAGCAAGAAGAAGCTGACAGCAGAGAAGTAGTCCTTCCTGGTGTTAATCTTGTTTTTGATGGCTCTGAGTTGCACCCCTTTGAGATAGGTGCTTGTCTACAGGCCCGTCAACCTGTCTCCTTAATAGCAGAGGCAGCTGCCGCCTCCGCAGCTCTGCAACAAAGCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAM91_C FAM91_N

Protein Analysis

810

Amino Acids

88.32

Weight (kDa)

5.08

Isoelectric Point (pI)

48.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAM91_N	PF14647	3 - 110	1.4e-22	FAM91 N-terminus
FAM91_C	PF14648	167 - 264	2.6e-21	FAM91 C-terminus
FAM91_C	PF14648	366 - 589	8.3e-31	FAM91 C-terminus
FAM91_C	PF14648	592 - 672	2.7e-11	FAM91 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35220 AT1G35220 AT1G35220 AT1G35220 AT1G35220 AT1G35220 AT1G35220
fragaria_vesca	FvH4_5g36450 FvH4_5g36450 FvH4_5g36450 FvH4_5g36450 FvH4_5g36450 FvH4_5g36450 FvH4_5g36450
malus_domestica	MD08G1188800.v1.1 MD15G1376600.v1.1 MD15G1376700.v1.1
prunus_persica	Prupe.1G523400_v2.0.a1
pyrus_communis	pycom08g16250
rosa_chinensis	RchiOBHm_Chr7g0238491
rosa_laevigata	RLG00000000910
rosa_multiflora	Rmu_sc0001536.1_g000012 Rmu_sc0025040.1_g000006
rosa_roxburghii	Rroxscaffold_3G00223010 Rroxscaffold_3G00223110
rosa_rugosa	Rorug07G0312600 Rorug07G0312700 Rorug07G0312800
rosa_samantha	Rh7AG467600 Rh7BG438300 Rh7CG485600 Rh7DG454100
rosa_wichuraiana	Rw7G038830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1245
Acc36I	ACCTGC	1 cut(s)	1235
AccI	GTMKAC	4 cut(s)	96, 460, 2070, 2356
AciI	CCGC	5 cut(s)	286, 323, 1382, 2400, 2406
AclWI	GGATC	4 cut(s)	179, 332, 382, 648
AcoI	YGGCCR	1 cut(s)	1386
AcsI	RAATTY	6 cut(s)	37, 665, 686, 1106, 1498, 1814
AcuI	CTGAAG	3 cut(s)	1029, 1083, 2169
AcyI	GRCGYC	1 cut(s)	1142
AfaI	GTAC	8 cut(s)	421, 649, 979, 1230, 1310, 1482, 1524, 1964
AfiI	CCNNNNNNNGG	6 cut(s)	547, 920, 1388, 1410, 2090, 2228
AhlI	ACTAGT	1 cut(s)	1300
AjnI	CCWGG	4 cut(s)	140, 370, 1234, 2296
Alw21I	GWGCWC	2 cut(s)	2236, 2249
Alw26I	GTCTC	6 cut(s)	395, 562, 1149, 1876, 2107, 2380
AlwI	GGATC	4 cut(s)	179, 332, 382, 648
AlwNI	CAGNNNCTG	2 cut(s)	347, 1979
Ama87I	CYCGRG	1 cut(s)	2098
AoxI	GGCC	5 cut(s)	813, 1262, 1386, 2219, 2361
ApeKI	GCWGC	9 cut(s)	283, 290, 293, 1531, 1982, 2210, 2393, 2396, 2408
ApoI	RAATTY	6 cut(s)	37, 665, 686, 1106, 1498, 1814
AseI	ATTAAT	2 cut(s)	971, 1254
Asp700I	GAANNNNTTC	1 cut(s)	729
AspS9I	GGNCC	6 cut(s)	1238, 1247, 1262, 1328, 1798, 2362
AsuC2I	CCSGG	1 cut(s)	564
AsuHPI	GGTGA	6 cut(s)	71, 461, 552, 988, 1567, 2254
AsuII	TTCGAA	1 cut(s)	1455
AvaI	CYCGRG	1 cut(s)	2098
AvaII	GGWCC	4 cut(s)	1238, 1247, 1328, 1798
AxyI	CCTNAGG	2 cut(s)	915, 1931
BanII	GRGCYC	1 cut(s)	2236
BarI	GAAGNNNNNNTAC	4 cut(s)	444, 476, 663, 695
BbsI	GAAGAC	1 cut(s)	68
Bbv12I	GWGCWC	2 cut(s)	2236, 2249
BbvI	GCAGC	9 cut(s)	270, 277, 280, 1543, 1969, 2197, 2383, 2405, 2420
BccI	CCATC	9 cut(s)	312, 541, 944, 956, 991, 1415, 2083, 2159, 2312
BciT130I	CCWGG	4 cut(s)	142, 372, 1236, 2298
BciVI	GTATCC	2 cut(s)	126, 903
BcnI	CCSGG	1 cut(s)	564
BcoDI	GTCTC	6 cut(s)	395, 562, 1149, 1876, 2107, 2380
BcuI	ACTAGT	1 cut(s)	1300
BfaI	CTAG	7 cut(s)	381, 456, 653, 1301, 1466, 2046, 2192
BfmI	CTRYAG	3 cut(s)	189, 680, 2357
BfuAI	ACCTGC	1 cut(s)	1235
BfuI	GTATCC	2 cut(s)	126, 903
BglI	GCCNNNNNGGC	1 cut(s)	1385
BlpI	GCTNAGC	1 cut(s)	427
Bme1390I	CCNGG	5 cut(s)	142, 372, 564, 1236, 2298
Bme18I	GGWCC	4 cut(s)	1238, 1247, 1328, 1798
BmeT110I	CYCGRG	1 cut(s)	2098
BmgT120I	GGNCC	6 cut(s)	1238, 1247, 1262, 1328, 1798, 2362
BmiI	GGNNCC	5 cut(s)	925, 1330, 1799, 1800, 2095
BmrFI	CCNGG	5 cut(s)	142, 372, 564, 1236, 2298
BmsI	GCATC	8 cut(s)	305, 353, 511, 820, 1044, 1906, 1918, 2143
BpiI	GAAGAC	1 cut(s)	68
BpmI	CTGGAG	1 cut(s)	1019
Bpu1102I	GCTNAGC	1 cut(s)	427
Bpu14I	TTCGAA	1 cut(s)	1455
BpuEI	CTTGAG	1 cut(s)	2176
BpuMI	CCSGG	1 cut(s)	564
Bsa29I	ATCGAT	1 cut(s)	1052
BsaHI	GRCGYC	1 cut(s)	1142
BsaI	GGTCTC	1 cut(s)	1876
BsaJI	CCNNGG	2 cut(s)	1289, 1578
BsaWI	WCCGGW	1 cut(s)	2239
Bsc4I	CCNNNNNNNGG	6 cut(s)	547, 920, 1388, 1410, 2090, 2228
Bse118I	RCCGGY	2 cut(s)	1388, 2130
Bse1I	ACTGG	2 cut(s)	255, 2222
Bse21I	CCTNAGG	2 cut(s)	915, 1931
Bse3DI	GCAATG	1 cut(s)	1926
BseBI	CCWGG	4 cut(s)	142, 372, 1236, 2298
BseCI	ATCGAT	1 cut(s)	1052
BseDI	CCNNGG	2 cut(s)	1289, 1578
BseGI	GGATG	7 cut(s)	296, 323, 533, 892, 1299, 1426, 2075
BseLI	CCNNNNNNNGG	6 cut(s)	547, 920, 1388, 1410, 2090, 2228
BseMI	GCAATG	1 cut(s)	1926
BseMII	CTCAG	7 cut(s)	441, 635, 906, 1552, 1637, 1896, 2316
BseNI	ACTGG	2 cut(s)	255, 2222
BseRI	GAGGAG	3 cut(s)	209, 756, 2226
BseXI	GCAGC	9 cut(s)	270, 277, 280, 1543, 1969, 2197, 2383, 2405, 2420
BsgI	GTGCAG	2 cut(s)	1550, 1977
BshFI	GGCC	5 cut(s)	815, 1264, 1388, 2221, 2363
BshVI	ATCGAT	1 cut(s)	1052
BsiHKAI	GWGCWC	2 cut(s)	2236, 2249
BsiHKCI	CYCGRG	1 cut(s)	2098
BsiSI	CCGG	4 cut(s)	563, 1389, 2131, 2240
BslFI	GGGAC	4 cut(s)	92, 493, 998, 1784
BslI	CCNNNNNNNGG	6 cut(s)	547, 920, 1388, 1410, 2090, 2228
BsmAI	GTCTC	6 cut(s)	395, 562, 1149, 1876, 2107, 2380
BsmBI	CGTCTC	1 cut(s)	1149
BsmFI	GGGAC	4 cut(s)	92, 493, 998, 1784
BsmI	GAATGC	3 cut(s)	238, 731, 1827
BsnI	GGCC	5 cut(s)	815, 1264, 1388, 2221, 2363
Bso31I	GGTCTC	1 cut(s)	1876
BsoBI	CYCGRG	1 cut(s)	2098
Bsp119I	TTCGAA	1 cut(s)	1455
Bsp1286I	GDGCHC	2 cut(s)	2236, 2249
Bsp143I	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
Bsp1720I	GCTNAGC	1 cut(s)	427
Bsp19I	CCATGG	1 cut(s)	1578
BspACI	CCGC	5 cut(s)	286, 323, 1382, 2400, 2406
BspANI	GGCC	5 cut(s)	815, 1264, 1388, 2221, 2363
BspCNI	CTCAG	7 cut(s)	440, 636, 907, 1551, 1638, 1897, 2317
BspDI	ATCGAT	1 cut(s)	1052
BspHI	TCATGA	2 cut(s)	1912, 2034
BspLI	GGNNCC	5 cut(s)	925, 1330, 1799, 1800, 2095
BspMI	ACCTGC	1 cut(s)	1235
BspPI	GGATC	4 cut(s)	179, 332, 382, 648
BspT104I	TTCGAA	1 cut(s)	1455
BspTNI	GGTCTC	1 cut(s)	1876
BsrDI	GCAATG	1 cut(s)	1926
BsrFI	RCCGGY	2 cut(s)	1388, 2130
BsrI	ACTGG	2 cut(s)	255, 2222
BssAI	RCCGGY	2 cut(s)	1388, 2130
BssECI	CCNNGG	2 cut(s)	1289, 1578
BssMI	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
BssNAI	GTATAC	1 cut(s)	97
BssNI	GRCGYC	1 cut(s)	1142
BssT1I	CCWWGG	2 cut(s)	1289, 1578
Bst1107I	GTATAC	1 cut(s)	97
Bst2UI	CCWGG	4 cut(s)	142, 372, 1236, 2298
Bst4CI	ACNGT	6 cut(s)	128, 241, 389, 1318, 1613, 1717
Bst6I	CTCTTC	1 cut(s)	1218
BstACI	GRCGYC	1 cut(s)	1142
BstAPI	GCANNNNNTGC	3 cut(s)	242, 302, 698
BstBI	TTCGAA	1 cut(s)	1455
BstC8I	GCNNGC	4 cut(s)	288, 579, 1059, 1386
BstDSI	CCRYGG	1 cut(s)	1578
BstENI	CCTNNNNNAGG	1 cut(s)	918
BstF5I	GGATG	7 cut(s)	296, 323, 533, 892, 1299, 1426, 2075
BstKTI	GATC	8 cut(s)	187, 340, 377, 502, 631, 643, 1168, 2183
BstMAI	GTCTC	6 cut(s)	395, 562, 1149, 1876, 2107, 2380
BstMBI	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
BstNI	CCWGG	4 cut(s)	142, 372, 1236, 2298
BstNSI	RCATGY	4 cut(s)	309, 581, 1061, 1326
BstSCI	CCNGG	5 cut(s)	140, 370, 562, 1234, 2296
BstSFI	CTRYAG	3 cut(s)	189, 680, 2357
BstV1I	GCAGC	9 cut(s)	270, 277, 280, 1543, 1969, 2197, 2383, 2405, 2420
BstV2I	GAAGAC	1 cut(s)	68
BstX2I	RGATCY	4 cut(s)	184, 337, 374, 640
BstYI	RGATCY	4 cut(s)	184, 337, 374, 640
BstZ17I	GTATAC	1 cut(s)	97
Bsu15I	ATCGAT	1 cut(s)	1052
Bsu36I	CCTNAGG	2 cut(s)	915, 1931
BsuI	GTATCC	2 cut(s)	126, 903
BsuRI	GGCC	5 cut(s)	815, 1264, 1388, 2221, 2363
BsuTUI	ATCGAT	1 cut(s)	1052
BtgI	CCRYGG	1 cut(s)	1578
BtsCI	GGATG	7 cut(s)	296, 323, 533, 892, 1299, 1426, 2075
BtsIMutI	CAGTG	1 cut(s)	1902
BveI	ACCTGC	1 cut(s)	1235
Cac8I	GCNNGC	4 cut(s)	288, 579, 1059, 1386
CaiI	CAGNNNCTG	2 cut(s)	347, 1979
CciI	TCATGA	2 cut(s)	1912, 2034
Cfr10I	RCCGGY	2 cut(s)	1388, 2130
Cfr13I	GGNCC	6 cut(s)	1238, 1247, 1262, 1328, 1798, 2362
ClaI	ATCGAT	1 cut(s)	1052
CseI	GACGC	2 cut(s)	1131, 1798
Csp6I	GTAC	8 cut(s)	420, 648, 978, 1229, 1309, 1481, 1523, 1963
CviQI	GTAC	8 cut(s)	420, 648, 978, 1229, 1309, 1481, 1523, 1963
DpnI	GATC	8 cut(s)	186, 339, 376, 501, 630, 642, 1167, 2182
DpnII	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
DraI	TTTAAA	3 cut(s)	133, 1497, 1747
DrdI	GACNNNNNNGTC	1 cut(s)	1245
DseDI	GACNNNNNNGTC	1 cut(s)	1245
EaeI	YGGCCR	1 cut(s)	1386
Eam1104I	CTCTTC	1 cut(s)	1218
EarI	CTCTTC	1 cut(s)	1218
Ecl136II	GAGCTC	1 cut(s)	2234
Eco130I	CCWWGG	2 cut(s)	1289, 1578
Eco24I	GRGCYC	1 cut(s)	2236
Eco31I	GGTCTC	1 cut(s)	1876
Eco32I	GATATC	2 cut(s)	994, 1120
Eco47I	GGWCC	4 cut(s)	1238, 1247, 1328, 1798
Eco53kI	GAGCTC	1 cut(s)	2234
Eco57I	CTGAAG	3 cut(s)	1029, 1083, 2169
Eco81I	CCTNAGG	2 cut(s)	915, 1931
Eco88I	CYCGRG	1 cut(s)	2098
EcoICRI	GAGCTC	1 cut(s)	2234
EcoNI	CCTNNNNNAGG	1 cut(s)	918
EcoO109I	RGGNCCY	2 cut(s)	1247, 1798
EcoRII	CCWGG	4 cut(s)	140, 370, 1234, 2296
EcoRV	GATATC	2 cut(s)	994, 1120
EcoT14I	CCWWGG	2 cut(s)	1289, 1578
EcoT22I	ATGCAT	3 cut(s)	703, 1059, 1283
EcoT38I	GRGCYC	1 cut(s)	2236
ErhI	CCWWGG	2 cut(s)	1289, 1578
Esp3I	CGTCTC	1 cut(s)	1149
FalI	AAGNNNNNCTT	2 cut(s)	2277, 2309
FaqI	GGGAC	4 cut(s)	92, 493, 998, 1784
FauI	CCCGC	2 cut(s)	279, 1389
FauNDI	CATATG	1 cut(s)	1856
FblI	GTMKAC	4 cut(s)	96, 460, 2070, 2356
FokI	GGATG	7 cut(s)	283, 330, 520, 899, 1306, 1433, 2062
FriOI	GRGCYC	1 cut(s)	2236
FspBI	CTAG	7 cut(s)	381, 456, 653, 1301, 1466, 2046, 2192
GsuI	CTGGAG	1 cut(s)	1019
HaeIII	GGCC	5 cut(s)	815, 1264, 1388, 2221, 2363
HapII	CCGG	4 cut(s)	563, 1389, 2131, 2240
HgaI	GACGC	2 cut(s)	1131, 1798
Hin1I	GRCGYC	1 cut(s)	1142
HincII	GTYRAC	2 cut(s)	2071, 2369
HindII	GTYRAC	2 cut(s)	2071, 2369
HindIII	AAGCTT	2 cut(s)	1135, 1158
HinfI	GANTC	4 cut(s)	1030, 1569, 1757, 2059
HpaII	CCGG	4 cut(s)	563, 1389, 2131, 2240
HphI	GGTGA	6 cut(s)	71, 461, 552, 988, 1567, 2254
Hpy188I	TCNGA	9 cut(s)	61, 645, 853, 988, 1112, 1347, 1509, 1762, 2326
Hpy99I	CGWCG	3 cut(s)	1228, 1814, 1879
HpyCH4III	ACNGT	6 cut(s)	128, 241, 389, 1318, 1613, 1717
HpyCH4IV	ACGT	1 cut(s)	1074
HpySE526I	ACGT	1 cut(s)	1074
Hsp92I	GRCGYC	1 cut(s)	1142
KflI	GGGWCCC	1 cut(s)	1798
Kzo9I	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
LmnI	GCTCC	4 cut(s)	169, 2093, 2231, 2239
Lsp1109I	GCAGC	9 cut(s)	270, 277, 280, 1543, 1969, 2197, 2383, 2405, 2420
LweI	GCATC	8 cut(s)	305, 353, 511, 820, 1044, 1906, 1918, 2143
MaeI	CTAG	7 cut(s)	381, 456, 653, 1301, 1466, 2046, 2192
MaeII	ACGT	1 cut(s)	1074
MaeIII	GTNAC	3 cut(s)	198, 583, 1181
MalI	GATC	8 cut(s)	186, 339, 376, 501, 630, 642, 1167, 2182
MboI	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
MfeI	CAATTG	2 cut(s)	311, 1431
MflI	RGATCY	4 cut(s)	184, 337, 374, 640
MhlI	GDGCHC	2 cut(s)	2236, 2249
Mph1103I	ATGCAT	3 cut(s)	703, 1059, 1283
MroXI	GAANNNNTTC	1 cut(s)	729
MslI	CAYNNNNRTG	4 cut(s)	501, 788, 1577, 1914
MspA1I	CMGCKG	3 cut(s)	283, 1384, 2396
MspI	CCGG	4 cut(s)	563, 1389, 2131, 2240
MspR9I	CCNGG	5 cut(s)	142, 372, 564, 1236, 2298
MunI	CAATTG	2 cut(s)	311, 1431
Mva1269I	GAATGC	3 cut(s)	238, 731, 1827
MvaI	CCWGG	4 cut(s)	142, 372, 1236, 2298
NciI	CCSGG	1 cut(s)	564
NcoI	CCATGG	1 cut(s)	1578
NdeI	CATATG	1 cut(s)	1856
NdeII	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
NlaIV	GGNNCC	5 cut(s)	925, 1330, 1799, 1800, 2095
NmuCI	GTSAC	2 cut(s)	583, 1181
NsiI	ATGCAT	3 cut(s)	703, 1059, 1283
NspI	RCATGY	4 cut(s)	309, 581, 1061, 1326
NspV	TTCGAA	1 cut(s)	1455
PaeI	GCATGC	2 cut(s)	581, 1061
PaeR7I	CTCGAG	1 cut(s)	2098
PagI	TCATGA	2 cut(s)	1912, 2034
PcsI	WCGNNNNNNNCGW	1 cut(s)	225
PctI	GAATGC	3 cut(s)	238, 731, 1827
PdmI	GAANNNNTTC	1 cut(s)	729
PfeI	GAWTC	4 cut(s)	1030, 1569, 1757, 2059
PfoI	TCCNGGA	1 cut(s)	1234
PpuMI	RGGWCCY	2 cut(s)	1247, 1798
PshBI	ATTAAT	2 cut(s)	971, 1254
Psp124BI	GAGCTC	1 cut(s)	2236
Psp5II	RGGWCCY	2 cut(s)	1247, 1798
Psp6I	CCWGG	4 cut(s)	140, 370, 1234, 2296
PspGI	CCWGG	4 cut(s)	140, 370, 1234, 2296
PspN4I	GGNNCC	5 cut(s)	925, 1330, 1799, 1800, 2095
PspPI	GGNCC	6 cut(s)	1238, 1247, 1262, 1328, 1798, 2362
PspPPI	RGGWCCY	2 cut(s)	1247, 1798
PspXI	VCTCGAGB	1 cut(s)	2098
PstNI	CAGNNNCTG	2 cut(s)	347, 1979
PsuI	RGATCY	4 cut(s)	184, 337, 374, 640
PvuII	CAGCTG	2 cut(s)	283, 2396
RsaI	GTAC	8 cut(s)	421, 649, 979, 1230, 1310, 1482, 1524, 1964
RsaNI	GTAC	8 cut(s)	420, 648, 978, 1229, 1309, 1481, 1523, 1963
RseI	CAYNNNNRTG	4 cut(s)	501, 788, 1577, 1914
SacI	GAGCTC	1 cut(s)	2236
SalI	GTCGAC	1 cut(s)	2069
Sau3AI	GATC	8 cut(s)	184, 337, 374, 499, 628, 640, 1165, 2180
Sau96I	GGNCC	6 cut(s)	1238, 1247, 1262, 1328, 1798, 2362
ScrFI	CCNGG	5 cut(s)	142, 372, 564, 1236, 2298
SduI	GDGCHC	2 cut(s)	2236, 2249
SfaNI	GCATC	8 cut(s)	305, 353, 511, 820, 1044, 1906, 1918, 2143
SfcI	CTRYAG	3 cut(s)	189, 680, 2357
Sfr274I	CTCGAG	1 cut(s)	2098
SfuI	TTCGAA	1 cut(s)	1455
SinI	GGWCC	4 cut(s)	1238, 1247, 1328, 1798
SlaI	CTCGAG	1 cut(s)	2098
SmiMI	CAYNNNNRTG	4 cut(s)	501, 788, 1577, 1914
SmlI	CTYRAG	2 cut(s)	2098, 2155
SmoI	CTYRAG	2 cut(s)	2098, 2155
SpeI	ACTAGT	1 cut(s)	1300
SphI	GCATGC	2 cut(s)	581, 1061
SsiI	CCGC	5 cut(s)	286, 323, 1382, 2400, 2406
SspI	AATATT	1 cut(s)	1514
SspMI	CTAG	7 cut(s)	381, 456, 653, 1301, 1466, 2046, 2192
SstI	GAGCTC	1 cut(s)	2236
StyD4I	CCNGG	5 cut(s)	140, 370, 562, 1234, 2296
StyI	CCWWGG	2 cut(s)	1289, 1578
TaaI	ACNGT	6 cut(s)	128, 241, 389, 1318, 1613, 1717
TaiI	ACGT	1 cut(s)	1077
TaqI	TCGA	9 cut(s)	147, 355, 882, 1052, 1455, 1554, 1812, 2070, 2099
TatI	WGTACW	4 cut(s)	419, 1308, 1480, 1522
TauI	GCSGC	1 cut(s)	2402
TfiI	GAWTC	4 cut(s)	1030, 1569, 1757, 2059
TscAI	CASTG	1 cut(s)	1909
TseFI	GTSAC	2 cut(s)	583, 1181
TseI	GCWGC	9 cut(s)	283, 290, 293, 1531, 1982, 2210, 2393, 2396, 2408
Tsp45I	GTSAC	2 cut(s)	583, 1181
TspDTI	ATGAA	9 cut(s)	195, 411, 609, 885, 1266, 1310, 1364, 1806, 2051
TspGWI	ACGGA	2 cut(s)	701, 916
TspRI	CASTG	1 cut(s)	1909
VpaK11BI	GGWCC	4 cut(s)	1238, 1247, 1328, 1798
VspI	ATTAAT	2 cut(s)	971, 1254
XagI	CCTNNNNNAGG	1 cut(s)	918
XapI	RAATTY	6 cut(s)	37, 665, 686, 1106, 1498, 1814
XceI	RCATGY	4 cut(s)	309, 581, 1061, 1326
XhoI	CTCGAG	1 cut(s)	2098
XmiI	GTMKAC	4 cut(s)	96, 460, 2070, 2356
XmnI	GAANNNNTTC	1 cut(s)	729
XspI	CTAG	7 cut(s)	381, 456, 653, 1301, 1466, 2046, 2192
Zsp2I	ATGCAT	3 cut(s)	703, 1059, 1283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.