MD15G1411100.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004811
Physical Location & Seq
Forward (+)
51047062 .. 51054889
7828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1411100.v1.1.491

Sequence Viewer

Length: 1686 bp
ATGCACGGCTACAGCCGGCTCGGCAATCCCCGAGCCGCGGGGTCCCCGCCGTCCTCCCCCCGCTGCCGCCACGGCCGCAACAAGAACGCCGCCGGCCGCCGAGGGCCCAAGCAGAGCATTCTGGAGAAGGTTGTGGTCTTGCTCATCTCGGTCGTTTTTAAGCGCCGCGCCGTGCTTTTGTTCGCGCCATTGTTGTACCTTTCGGGGATGTTGCTGTATATGGGCACCGTGAACTTTGATGTAGTTAGCTTGAAAAATGCGGTTGTTGTGGTTAGTAAGCGGTCGCCTCCGGGCTCAGTGTATAGGAGCCCTCAGGTTTTTCACAAGCTTTGGCCTCATATGCAGGCTGAGAGCAATCACACTCACAATTTGTTGATGTCGGTGTGGAATTCAAAAGTGCGGCAAGTTTGGAAGCCTTGTATTAGCAACGATATTTCAAATGAAGGACTACCAAAGTCAAATGGTTTCTTTATAATTGAAGCAAATGGTGGATTGAACCAGCAACGTTTGTCGATTTGTGATGCAGTTGCCGTGGCTGGACTATTGAATGCAACGCTTCTGATTCCAATTTTCCATCTAAATAGTGTCTGGAAAGACTCGAGCCACTTTGGGAACATATTTGATGAAGATTTCTTCATTCATGCTCTCAGAAATCACGTGAATGTGGTTAGGGAGCTCCCAGGGGATATACTTGAGCGATTTGACAATAATATAAGCAACATTGTGAATCTGAGAGTGAAAGGTTGGTCAAGTCCAGCTTACTATCTTCAAAAGGTCCTCCCAAAGTTGGAACAAATGGGGGCCGTGCGAATTGCACCTTTTTCCAACAGATTGGCTTATGCAGTTCCTTCAAAGGTCCAAAGGCTTAGATGCTTAGCAAATTTTGAAACACTGAGGTTTTCAAATTCTATAAGAACGCTTGCCGATAAAATGGTTGAAAGAATGGTTAAAAGTAGCTCCCACAGAGGGGGAAAATATGTTTCAGTGCATCTTCGATTTGAAGAGGACATGGTAGCATTTTCGTGCTGTGAATATGATGGTGGGGAGGAAGAGAAACACGAAATGGATATTGCTCGAGAAAGAAGTTGGAGAGGAAAATTCAGGAAAAGGGGTAGAGTAATAAGGCCAGGTGCAAATCGTGTGGATGGGAAGTGCCCTTTAACTCCATTAGAGGTAGGAATGATGCTCAGGGGTATGGGATTTGATAATACCACCTCAGTTTACGTTGCAGCTGGAAAAATTTATAAAGCAGAGAAGTACATGGCTCCTCTTAAACAGATGTTTCCACTCTTGGAAATGAAAGATACACTGGCAACTGCAGAAGAACTTGCTCCTTTTAAGGGTCACTTATCTAGGTTGGCGGCTCTTGATTACACGGTTTGCCTCCATAGTGAAGTATTTGTCACCACTCAGGGAGGAAATTTCCCCCACTTCTTGATGGGTCACAGGCGGTATCTCTATGGAGGACATTCAAAGACAATCAAGCCTGATAAAAGAAAATTAGCACTTTTGTTTGATAATCCCAATATCAGATGGGAAGATTTCAAACGGCAGATGCAAGACATGCTTCGCCACAGTGACCAAAAGGGTGTTGAATTGAAAAAACCCAGTGCGTCGCTGTATACATTTCCCATGCCAGATTGTATGTGTAAAGAAGCAGACGCCAGAAATGAAAGGGGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

562

Amino Acids

63.58

Weight (kDa)

9.79

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 157 - 486 2.6e-99 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 473, 1245
AccB1I GGYRCC 1 cut(s) 224
AccI GTMKAC 1 cut(s) 1622
AccII CGCG 3 cut(s) 38, 168, 185
AclI AACGTT 1 cut(s) 505
AcoI YGGCCR 2 cut(s) 73, 94
AcsI RAATTY 6 cut(s) 388, 880, 904, 1097, 1239, 1420
AcvI CACGTG 1 cut(s) 658
AcyI GRCGYC 1 cut(s) 1662
AfaI GTAC 2 cut(s) 197, 1259
AfiI CCNNNNNNNGG 5 cut(s) 37, 787, 966, 967, 1340
AjnI CCWGG 2 cut(s) 679, 1126
AluBI AGCT 6 cut(s) 249, 328, 676, 758, 957, 1232
AluI AGCT 6 cut(s) 249, 328, 676, 758, 957, 1232
Alw21I GWGCWC 1 cut(s) 678
Ama87I CYCGRG 3 cut(s) 30, 598, 1074
AoxI GGCC 6 cut(s) 73, 94, 104, 332, 801, 1124
ApaI GGGCCC 1 cut(s) 108
ApeKI GCWGC 2 cut(s) 63, 1229
ApoI RAATTY 6 cut(s) 388, 880, 904, 1097, 1239, 1420
AspLEI GCGC 3 cut(s) 165, 170, 187
AspS9I GGNCC 6 cut(s) 42, 104, 105, 775, 801, 856
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 1 cut(s) 1396
AvaI CYCGRG 3 cut(s) 30, 598, 1074
AvaII GGWCC 3 cut(s) 42, 775, 856
AxyI CCTNAGG 1 cut(s) 312
BaeGI GKGCMC 3 cut(s) 108, 227, 1157
BanI GGYRCC 1 cut(s) 224
BanII GRGCYC 4 cut(s) 108, 296, 311, 678
BbrPI CACGTG 1 cut(s) 658
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 2 cut(s) 50, 1241
BccI CCATC 5 cut(s) 582, 1031, 1139, 1432, 1527
BceAI ACGGC 7 cut(s) 22, 34, 88, 155, 515, 788, 1565
BciT130I CCWGG 2 cut(s) 681, 1128
BcnI CCSGG 1 cut(s) 291
BfaI CTAG 1 cut(s) 1353
BfmI CTRYAG 2 cut(s) 10, 1317
BfoI RGCGCY 1 cut(s) 166
BglI GCCNNNNNGGC 2 cut(s) 21, 72
BlpI GCTNAGC 1 cut(s) 874
Bme1390I CCNGG 3 cut(s) 291, 681, 1128
Bme18I GGWCC 3 cut(s) 42, 775, 856
BmeT110I CYCGRG 3 cut(s) 30, 598, 1074
BmgT120I GGNCC 6 cut(s) 42, 104, 105, 775, 801, 856
BmiI GGNNCC 7 cut(s) 43, 44, 106, 226, 308, 802, 1266
BmrFI CCNGG 3 cut(s) 291, 681, 1128
BmrI ACTGGG 1 cut(s) 1602
BmsI GCATC 5 cut(s) 511, 860, 997, 1173, 1545
BmuI ACTGGG 1 cut(s) 1602
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 1187
Bpu1102I GCTNAGC 1 cut(s) 874
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 291
BsaAI YACGTR 1 cut(s) 658
BsaHI GRCGYC 1 cut(s) 1662
BsaJI CCNNGG 6 cut(s) 36, 70, 100, 531, 679, 680
Bsc4I CCNNNNNNNGG 5 cut(s) 37, 787, 966, 967, 1340
Bse118I RCCGGY 2 cut(s) 15, 92
Bse1I ACTGG 2 cut(s) 1314, 1608
Bse21I CCTNAGG 1 cut(s) 312
BseBI CCWGG 2 cut(s) 681, 1128
BseDI CCNNGG 6 cut(s) 36, 70, 100, 531, 679, 680
BseGI GGATG 2 cut(s) 213, 1150
BseLI CCNNNNNNNGG 5 cut(s) 37, 787, 966, 967, 1340
BseMII CTCAG 9 cut(s) 309, 326, 339, 661, 722, 884, 1201, 1230, 1424
BseNI ACTGG 2 cut(s) 1314, 1608
BseRI GAGGAG 1 cut(s) 1257
BseSI GKGCMC 3 cut(s) 108, 227, 1157
BseX3I CGGCCG 2 cut(s) 73, 94
BseXI GCAGC 2 cut(s) 50, 1241
Bsh1236I CGCG 3 cut(s) 38, 168, 185
Bsh1285I CGRYCG 4 cut(s) 76, 97, 153, 284
BshFI GGCC 6 cut(s) 75, 96, 106, 334, 803, 1126
BshNI GGYRCC 1 cut(s) 224
BsiEI CGRYCG 4 cut(s) 76, 97, 153, 284
BsiHKAI GWGCWC 1 cut(s) 678
BsiHKCI CYCGRG 3 cut(s) 30, 598, 1074
BsiSI CCGG 3 cut(s) 16, 93, 290
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 5 cut(s) 37, 787, 966, 967, 1340
BsmFI GGGAC 1 cut(s) 28
BsmI GAATGC 2 cut(s) 117, 553
BsnI GGCC 6 cut(s) 75, 96, 106, 334, 803, 1126
BsoBI CYCGRG 3 cut(s) 30, 598, 1074
Bsp120I GGGCCC 1 cut(s) 104
Bsp1286I GDGCHC 6 cut(s) 108, 227, 296, 311, 678, 1157
Bsp1720I GCTNAGC 1 cut(s) 874
BspANI GGCC 6 cut(s) 75, 96, 106, 334, 803, 1126
BspCNI CTCAG 9 cut(s) 308, 325, 340, 660, 723, 885, 1200, 1229, 1423
BspFNI CGCG 3 cut(s) 38, 168, 185
BspLI GGNNCC 7 cut(s) 43, 44, 106, 226, 308, 802, 1266
BspMAI CTGCAG 1 cut(s) 1321
BspT107I GGYRCC 1 cut(s) 224
BsrFI RCCGGY 2 cut(s) 15, 92
BsrI ACTGG 2 cut(s) 1314, 1608
BssAI RCCGGY 2 cut(s) 15, 92
BssECI CCNNGG 6 cut(s) 36, 70, 100, 531, 679, 680
BssNAI GTATAC 1 cut(s) 1623
BssNI GRCGYC 1 cut(s) 1662
Bst1107I GTATAC 1 cut(s) 1623
Bst2UI CCWGG 2 cut(s) 681, 1128
Bst4CI ACNGT 3 cut(s) 229, 1378, 1577
Bst6I CTCTTC 2 cut(s) 996, 1044
BstACI GRCGYC 1 cut(s) 1662
BstAPI GCANNNNNTGC 1 cut(s) 1564
BstBAI YACGTR 1 cut(s) 658
BstC8I GCNNGC 4 cut(s) 17, 94, 345, 921
BstDSI CCRYGG 3 cut(s) 36, 70, 531
BstF5I GGATG 2 cut(s) 213, 1150
BstFNI CGCG 3 cut(s) 38, 168, 185
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 3 cut(s) 165, 170, 187
BstMCI CGRYCG 4 cut(s) 76, 97, 153, 284
BstMWI GCNNNNNNNGC 5 cut(s) 21, 72, 75, 340, 1564
BstNI CCWGG 2 cut(s) 681, 1128
BstNSI RCATGY 1 cut(s) 1567
BstSCI CCNGG 3 cut(s) 289, 679, 1126
BstSFI CTRYAG 2 cut(s) 10, 1317
BstSLI GKGCMC 3 cut(s) 108, 227, 1157
BstUI CGCG 3 cut(s) 38, 168, 185
BstV1I GCAGC 2 cut(s) 50, 1241
BstXI CCANNNNNNTGG 1 cut(s) 832
BstZ17I GTATAC 1 cut(s) 1623
BstZI CGGCCG 2 cut(s) 73, 94
Bsu36I CCTNAGG 1 cut(s) 312
BsuRI GGCC 6 cut(s) 75, 96, 106, 334, 803, 1126
BtgI CCRYGG 3 cut(s) 36, 70, 531
BtsCI GGATG 2 cut(s) 213, 1150
BtsIMutI CAGTG 6 cut(s) 303, 890, 990, 1307, 1582, 1615
Cac8I GCNNGC 4 cut(s) 17, 94, 345, 921
CfoI GCGC 3 cut(s) 165, 170, 187
Cfr10I RCCGGY 2 cut(s) 15, 92
Cfr13I GGNCC 6 cut(s) 42, 104, 105, 775, 801, 856
Cfr42I CCGCGG 1 cut(s) 39
CseI GACGC 2 cut(s) 1602, 1670
Csp6I GTAC 2 cut(s) 196, 1258
CspCI CAANNNNNGTGG 2 cut(s) 1122, 1157
CviAII CATG 5 cut(s) 641, 1009, 1261, 1564, 1633
CviQI GTAC 2 cut(s) 196, 1258
EaeI YGGCCR 2 cut(s) 73, 94
EagI CGGCCG 2 cut(s) 73, 94
Eam1104I CTCTTC 2 cut(s) 996, 1044
EarI CTCTTC 2 cut(s) 996, 1044
Ecl136II GAGCTC 1 cut(s) 676
EclXI CGGCCG 2 cut(s) 73, 94
Eco24I GRGCYC 4 cut(s) 108, 296, 311, 678
Eco47I GGWCC 3 cut(s) 42, 775, 856
Eco52I CGGCCG 2 cut(s) 73, 94
Eco53kI GAGCTC 1 cut(s) 676
Eco72I CACGTG 1 cut(s) 658
Eco81I CCTNAGG 1 cut(s) 312
Eco88I CYCGRG 3 cut(s) 30, 598, 1074
EcoICRI GAGCTC 1 cut(s) 676
EcoO109I RGGNCCY 3 cut(s) 42, 104, 775
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 679, 1126
EcoT38I GRGCYC 4 cut(s) 108, 296, 311, 678
FaeI CATG 5 cut(s) 644, 1012, 1264, 1567, 1636
FalI AAGNNNNNCTT 6 cut(s) 742, 774, 1331, 1363, 1551, 1583
FaqI GGGAC 1 cut(s) 28
FatI CATG 5 cut(s) 640, 1008, 1260, 1563, 1632
FauI CCCGC 3 cut(s) 31, 54, 68
FauNDI CATATG 1 cut(s) 339
FblI GTMKAC 1 cut(s) 1622
FokI GGATG 2 cut(s) 220, 1157
FriOI GRGCYC 4 cut(s) 108, 296, 311, 678
FspBI CTAG 1 cut(s) 1353
GlaI GCGC 3 cut(s) 164, 169, 186
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 6 cut(s) 75, 96, 106, 334, 803, 1126
HapII CCGG 3 cut(s) 16, 93, 290
HgaI GACGC 2 cut(s) 1602, 1670
HhaI GCGC 3 cut(s) 165, 170, 187
Hin1I GRCGYC 1 cut(s) 1662
Hin1II CATG 5 cut(s) 644, 1012, 1264, 1567, 1636
Hin6I GCGC 3 cut(s) 163, 168, 185
HinP1I GCGC 3 cut(s) 163, 168, 185
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 3 cut(s) 562, 596, 727
HpaII CCGG 3 cut(s) 16, 93, 290
HphI GGTGA 1 cut(s) 1396
Hpy166II GTNNAC 3 cut(s) 232, 1222, 1623
Hpy188I TCNGA 4 cut(s) 561, 650, 732, 1532
Hpy188III TCNNGA 6 cut(s) 122, 589, 1076, 1102, 1367, 1435
Hpy8I GTNNAC 3 cut(s) 232, 1222, 1623
Hpy99I CGWCG 1 cut(s) 1618
HpyAV CCTTC 3 cut(s) 121, 437, 858
HpyCH4III ACNGT 3 cut(s) 229, 1378, 1577
HpyCH4IV ACGT 3 cut(s) 505, 657, 1224
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 72, 75, 340, 1564
HpySE526I ACGT 3 cut(s) 505, 657, 1224
Hsp92I GRCGYC 1 cut(s) 1662
Hsp92II CATG 5 cut(s) 644, 1012, 1264, 1567, 1636
HspAI GCGC 3 cut(s) 163, 168, 185
KflI GGGWCCC 1 cut(s) 42
KroI GCCGGC 2 cut(s) 15, 92
KroNI GCCGGC 2 cut(s) 17, 94
KspI CCGCGG 1 cut(s) 39
LmnI GCTCC 6 cut(s) 306, 673, 681, 962, 1270, 1336
Lsp1109I GCAGC 2 cut(s) 50, 1241
LweI GCATC 5 cut(s) 511, 860, 997, 1173, 1545
MaeI CTAG 1 cut(s) 1353
MaeII ACGT 3 cut(s) 505, 657, 1224
MaeIII GTNAC 5 cut(s) 1343, 1402, 1442, 1577, 1679
MboII GAAGA 8 cut(s) 625, 638, 758, 983, 1013, 1061, 1334, 1550
MhlI GDGCHC 6 cut(s) 108, 227, 296, 311, 678, 1157
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 3 cut(s) 768, 849, 1067
MroNI GCCGGC 2 cut(s) 15, 92
MseI TTAA 5 cut(s) 159, 948, 1160, 1272, 1338
MslI CAYNNNNRTG 2 cut(s) 660, 1021
MspA1I CMGCKG 3 cut(s) 38, 63, 1232
MspI CCGG 3 cut(s) 16, 93, 290
MspR9I CCNGG 3 cut(s) 291, 681, 1128
MteI GCGCNGCGC 1 cut(s) 166
Mva1269I GAATGC 2 cut(s) 117, 553
MvaI CCWGG 2 cut(s) 681, 1128
MvnI CGCG 3 cut(s) 38, 168, 185
MwoI GCNNNNNNNGC 5 cut(s) 21, 72, 75, 340, 1564
NaeI GCCGGC 2 cut(s) 17, 94
NciI CCSGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 339
NgoMIV GCCGGC 2 cut(s) 15, 92
NlaIII CATG 5 cut(s) 644, 1012, 1264, 1567, 1636
NlaIV GGNNCC 7 cut(s) 43, 44, 106, 226, 308, 802, 1266
NmeAIII GCCGAG 1 cut(s) 125
NmuCI GTSAC 4 cut(s) 1343, 1402, 1442, 1577
NspI RCATGY 1 cut(s) 1567
PaeR7I CTCGAG 2 cut(s) 598, 1074
PasI CCCWGGG 1 cut(s) 680
PctI GAATGC 2 cut(s) 117, 553
PdiI GCCGGC 2 cut(s) 17, 94
PfeI GAWTC 2 cut(s) 562, 727
PleI GAGTC 1 cut(s) 590
PmaCI CACGTG 1 cut(s) 658
PmlI CACGTG 1 cut(s) 658
PpsI GAGTC 1 cut(s) 590
Ppu21I YACGTR 1 cut(s) 658
PpuMI RGGWCCY 2 cut(s) 42, 775
PsiI TTATAA 2 cut(s) 473, 1245
Psp124BI GAGCTC 1 cut(s) 678
Psp1406I AACGTT 1 cut(s) 505
Psp5II RGGWCCY 2 cut(s) 42, 775
Psp6I CCWGG 2 cut(s) 679, 1126
PspCI CACGTG 1 cut(s) 658
PspGI CCWGG 2 cut(s) 679, 1126
PspN4I GGNNCC 7 cut(s) 43, 44, 106, 226, 308, 802, 1266
PspOMI GGGCCC 1 cut(s) 104
PspPI GGNCC 6 cut(s) 42, 104, 105, 775, 801, 856
PspPPI RGGWCCY 2 cut(s) 42, 775
PspXI VCTCGAGB 1 cut(s) 598
PstI CTGCAG 1 cut(s) 1321
PvuII CAGCTG 1 cut(s) 1232
RsaI GTAC 2 cut(s) 197, 1259
RsaNI GTAC 2 cut(s) 196, 1258
RseI CAYNNNNRTG 2 cut(s) 660, 1021
SacI GAGCTC 1 cut(s) 678
SacII CCGCGG 1 cut(s) 39
SaqAI TTAA 5 cut(s) 159, 948, 1160, 1272, 1338
Sau96I GGNCC 6 cut(s) 42, 104, 105, 775, 801, 856
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 3 cut(s) 291, 681, 1128
SduI GDGCHC 6 cut(s) 108, 227, 296, 311, 678, 1157
SfaNI GCATC 5 cut(s) 511, 860, 997, 1173, 1545
SfcI CTRYAG 2 cut(s) 10, 1317
Sfr274I CTCGAG 2 cut(s) 598, 1074
Sfr303I CCGCGG 1 cut(s) 39
SgrBI CCGCGG 1 cut(s) 39
SinI GGWCC 3 cut(s) 42, 775, 856
SlaI CTCGAG 2 cut(s) 598, 1074
SmiMI CAYNNNNRTG 2 cut(s) 660, 1021
SmlI CTYRAG 3 cut(s) 598, 692, 1074
SmoI CTYRAG 3 cut(s) 598, 692, 1074
SspMI CTAG 1 cut(s) 1353
SstI GAGCTC 1 cut(s) 678
StyD4I CCNGG 3 cut(s) 289, 679, 1126
TaaI ACNGT 3 cut(s) 229, 1378, 1577
TaiI ACGT 3 cut(s) 508, 660, 1227
TaqI TCGA 4 cut(s) 512, 599, 994, 1075
TaqII GACCGA 1 cut(s) 139
TatI WGTACW 1 cut(s) 1257
TauI GCSGC 8 cut(s) 38, 69, 78, 92, 99, 168, 403, 1364
TfiI GAWTC 2 cut(s) 562, 727
Tru1I TTAA 5 cut(s) 159, 948, 1160, 1272, 1338
Tru9I TTAA 5 cut(s) 159, 948, 1160, 1272, 1338
TscAI CASTG 6 cut(s) 303, 897, 990, 1314, 1582, 1615
TseFI GTSAC 4 cut(s) 1343, 1402, 1442, 1577
TseI GCWGC 2 cut(s) 63, 1229
Tsp45I GTSAC 4 cut(s) 1343, 1402, 1442, 1577
TspDTI ATGAA 6 cut(s) 456, 625, 629, 639, 1313, 1686
TspRI CASTG 6 cut(s) 303, 897, 990, 1314, 1582, 1615
VpaK11BI GGWCC 3 cut(s) 42, 775, 856
XapI RAATTY 6 cut(s) 388, 880, 904, 1097, 1239, 1420
XceI RCATGY 1 cut(s) 1567
XhoI CTCGAG 2 cut(s) 598, 1074
XmiI GTMKAC 1 cut(s) 1622
XspI CTAG 1 cut(s) 1353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.