Rh7AG403500

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004811
Physical Location & Seq
Reverse (-)
53291178 .. 53298285
7108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG403500.1

Sequence Viewer

Length: 1479 bp
ATGGGCTCTGTGAGCTTCGATGTAGTTAGCTTGAAGAACGTTGTGATTATCAGTAAGCCGTCGCCGCCGGGATCACTGTACAGGAGCCCTCAGGTTTTTCAGAAGCTCTGGCCTTATATGCAGGCCGAGAGTAATCACACTCCCAATTTGTTGATGACAGTGTGGAACTCAAATGTGCATAAAGTTTGGAAGCCTTGTATTCCCCAAGATACTCCGAAAGCAGAATTACCAAAGTCAAATGGTCTGTTCATAATTGAAGCGAACGGTGGGTTGAACCAGCAACGGCTGTCGATATGTGATGCAGTTGCTGTGGCTGGACTCCTAAATGCAACTCTTCTGATTCCAATCTTTCATCTAAATAGTGTTTGGAGAGACTCAAGTCACTTTGGGAACATATTTGATGAAGATTTCTTCATCCATGCTCTCAGAAATCACGTTAATGTGGTCAGGGAACTTCCAGAAGATATACTTCAGCGGTTTGACAATAATATAAGCAACATTGTGAATTTGAGAGTGAAAGGTTGGTCAAGTTCAGCTCACTATCTCCGTAAGGTCCTCCCGAAGTTAGAGGAGATGGGGGCTGTGCGCATTGCACCTTTTTCAAACAGATTGGCTCATGCAGTTCCTTCAAACATCCAGAGCCTTAGATGCTTTGCCAATTTTGAAGCACTGAGGTTCTCAAATTCAATAAGACTGCTTGCAGATAAAATGGTTGATCGAATGGTCAAAAGTAGCTCCCACAGTGAAGGCAAATATGTTTCAGTACATCTTCGCTTTGAAGAGGACATGGTTGCATTTTCATGCTGTGAATATGATGGTGGGGAGGAAGAGAAACATGAAATGGATATTGCTCGAGAAAGAAGCTGGAGAGGAAAATTCAGGAGAAGGGGTAGAGTAATTAGGCCAGGTGCAAATCGTGTTGATGGGAAATGCCCTTTAACGCCATTAGAGGTGGGAATGATGCTCAGGGGCATGGGATTTGATAATACCACCTCAGTATACATTGCAGCAGGTAAAATTTATAAAGCAGAAAAGTACATGGCTCCACTTAAACAGATGTTTCCACGCTTGCAAATGAAAGACACACTGGCTACTTCAGAAGAACTTGCTCCTTTTAAGGGCCACTTATCTAGGTTGGCTGCTCTTGATTACACGGTTTGCCTCCATAGTGAAGTGTTTGTCACCACTCAGGGGGGTAATTTTCCCCACTTTTTGATGGGCCACAGGCGCTATCTCTATGGAGGACATTCGAAGACAATCAAGCCTGATAAGCGTAAATTGGCACTGTTGTTTGATAATCCCAGTATCAGATGGGAAGATTTCAAGCGACAGATGCAAGACATGCTCAGACACAGTGAGCAGAAGGGTGTTGAAGTGAGAAAACCCAGTGCCTCACTATATACATTTCCCATGCCAGATTGTATGTGTAAGGAAGGAGAAGCTATAGATGAAAACAGTAACACAACAACACAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

492

Amino Acids

55.98

Weight (kDa)

9.18

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 85 - 412 9.6e-100 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
Acc16I TGCGCA 1 cut(s) 587
Acc36I ACCTGC 1 cut(s) 1001
AccI GTMKAC 1 cut(s) 999
AciI CCGC 2 cut(s) 65, 475
AclI AACGTT 1 cut(s) 39
AclWI GGATC 1 cut(s) 79
AcsI RAATTY 4 cut(s) 505, 682, 875, 1017
AcuI CTGAAG 2 cut(s) 455, 1080
AfaI GTAC 3 cut(s) 80, 765, 1037
AfiI CCNNNNNNNGG 2 cut(s) 1118, 1191
AjnI CCWGG 1 cut(s) 904
AluBI AGCT 7 cut(s) 15, 30, 106, 536, 735, 864, 1442
AluI AGCT 7 cut(s) 15, 30, 106, 536, 735, 864, 1442
Alw26I GTCTC 1 cut(s) 366
AlwI GGATC 1 cut(s) 79
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 852
AoxI GGCC 5 cut(s) 110, 123, 902, 1120, 1219
ApeKI GCWGC 2 cut(s) 1007, 1139
ApoI RAATTY 4 cut(s) 505, 682, 875, 1017
AspLEI GCGC 2 cut(s) 588, 1230
AspS9I GGNCC 3 cut(s) 553, 1120, 1219
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 1174
AsuII TTCGAA 1 cut(s) 1250
AvaI CYCGRG 1 cut(s) 852
AvaII GGWCC 1 cut(s) 553
AxyI CCTNAGG 1 cut(s) 90
BanII GRGCYC 2 cut(s) 8, 89
BbsI GAAGAC 1 cut(s) 1259
BbvI GCAGC 2 cut(s) 1019, 1126
BccI CCATC 5 cut(s) 568, 809, 917, 1210, 1305
BceAI ACGGC 2 cut(s) 43, 299
BciT130I CCWGG 1 cut(s) 906
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 366
BfaI CTAG 1 cut(s) 1131
BfmI CTRYAG 1 cut(s) 1443
BfoI RGCGCY 1 cut(s) 1231
BfuAI ACCTGC 1 cut(s) 1001
BisI GCNGC 3 cut(s) 65, 1008, 1140
BlsI GCNGC 3 cut(s) 66, 1009, 1141
Bme1390I CCNGG 2 cut(s) 69, 906
Bme18I GGWCC 1 cut(s) 553
BmeT110I CYCGRG 1 cut(s) 852
BmgT120I GGNCC 3 cut(s) 553, 1120, 1219
BmiI GGNNCC 2 cut(s) 86, 1044
BmrFI CCNGG 2 cut(s) 69, 906
BmrI ACTGGG 2 cut(s) 1296, 1380
BmsI GCATC 4 cut(s) 289, 638, 951, 1323
BmuI ACTGGG 2 cut(s) 1296, 1380
BoxI GACNNNNGTC 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 1259
BpmI CTGGAG 1 cut(s) 886
Bpu10I CCTNAGC 1 cut(s) 965
Bpu14I TTCGAA 1 cut(s) 1250
BpuEI CTTGAG 1 cut(s) 361
BpuMI CCSGG 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 874, 904
Bsc4I CCNNNNNNNGG 2 cut(s) 1118, 1191
Bse1I ACTGG 3 cut(s) 1092, 1302, 1386
Bse21I CCTNAGG 1 cut(s) 90
Bse3DI GCAATG 2 cut(s) 588, 1002
Bse8I GATNNNNATC 1 cut(s) 344
BseBI CCWGG 1 cut(s) 906
BseGI GGATG 2 cut(s) 414, 633
BseJI GATNNNNATC 1 cut(s) 344
BseLI CCNNNNNNNGG 2 cut(s) 1118, 1191
BseMI GCAATG 2 cut(s) 588, 1002
BseMII CTCAG 7 cut(s) 104, 439, 662, 979, 1008, 1202, 1360
BseNI ACTGG 3 cut(s) 1092, 1302, 1386
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 2 cut(s) 1019, 1126
BshFI GGCC 5 cut(s) 112, 125, 904, 1122, 1221
BsiHKCI CYCGRG 1 cut(s) 852
BsiSI CCGG 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 1118, 1191
BsmAI GTCTC 1 cut(s) 366
BsnI GGCC 5 cut(s) 112, 125, 904, 1122, 1221
BsoBI CYCGRG 1 cut(s) 852
Bsp119I TTCGAA 1 cut(s) 1250
Bsp1286I GDGCHC 2 cut(s) 8, 89
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 2 cut(s) 71, 715
BspACI CCGC 2 cut(s) 65, 475
BspANI GGCC 5 cut(s) 112, 125, 904, 1122, 1221
BspCNI CTCAG 7 cut(s) 103, 438, 663, 978, 1007, 1201, 1359
BspLI GGNNCC 2 cut(s) 86, 1044
BspMI ACCTGC 1 cut(s) 1001
BspPI GGATC 1 cut(s) 79
BspT104I TTCGAA 1 cut(s) 1250
BsrDI GCAATG 2 cut(s) 588, 1002
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 3 cut(s) 1092, 1302, 1386
BssMI GATC 2 cut(s) 71, 715
BssNAI GTATAC 1 cut(s) 1000
Bst1107I GTATAC 1 cut(s) 1000
Bst2UI CCWGG 1 cut(s) 906
Bst4CI ACNGT 8 cut(s) 78, 160, 266, 743, 1156, 1287, 1355, 1457
Bst6I CTCTTC 3 cut(s) 339, 774, 822
BstAPI GCANNNNNTGC 1 cut(s) 1342
BstAUI TGTACA 1 cut(s) 78
BstBI TTCGAA 1 cut(s) 1250
BstC8I GCNNGC 3 cut(s) 123, 699, 1070
BstDEI CTNAG 8 cut(s) 90, 425, 644, 671, 965, 994, 1188, 1346
BstF5I GGATG 2 cut(s) 414, 633
BstH2I RGCGCY 1 cut(s) 1231
BstHHI GCGC 2 cut(s) 588, 1230
BstKTI GATC 2 cut(s) 74, 718
BstMAI GTCTC 1 cut(s) 366
BstMBI GATC 2 cut(s) 71, 715
BstMWI GCNNNNNNNGC 8 cut(s) 12, 64, 118, 648, 1227, 1270, 1333, 1342
BstNI CCWGG 1 cut(s) 906
BstNSI RCATGY 1 cut(s) 1345
BstPAI GACNNNNGTC 1 cut(s) 378
BstSCI CCNGG 2 cut(s) 67, 904
BstSFI CTRYAG 1 cut(s) 1443
BstV1I GCAGC 2 cut(s) 1019, 1126
BstV2I GAAGAC 1 cut(s) 1259
BstZ17I GTATAC 1 cut(s) 1000
Bsu36I CCTNAGG 1 cut(s) 90
BsuRI GGCC 5 cut(s) 112, 125, 904, 1122, 1221
BtsCI GGATG 2 cut(s) 414, 633
BtsIMutI CAGTG 8 cut(s) 74, 165, 668, 748, 1085, 1283, 1360, 1393
BveI ACCTGC 1 cut(s) 1001
Cac8I GCNNGC 3 cut(s) 123, 699, 1070
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 2 cut(s) 588, 1230
Cfr13I GGNCC 3 cut(s) 553, 1120, 1219
Csp6I GTAC 3 cut(s) 79, 764, 1036
CviAII CATG 9 cut(s) 419, 617, 787, 801, 836, 973, 1039, 1342, 1411
CviQI GTAC 3 cut(s) 79, 764, 1036
DdeI CTNAG 8 cut(s) 90, 425, 644, 671, 965, 994, 1188, 1346
DpnI GATC 2 cut(s) 73, 717
DpnII GATC 2 cut(s) 71, 715
Eam1104I CTCTTC 3 cut(s) 339, 774, 822
EarI CTCTTC 3 cut(s) 339, 774, 822
Eco24I GRGCYC 2 cut(s) 8, 89
Eco47I GGWCC 1 cut(s) 553
Eco57I CTGAAG 2 cut(s) 455, 1080
Eco81I CCTNAGG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 852
EcoO109I RGGNCCY 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 904
EcoT38I GRGCYC 2 cut(s) 8, 89
FaeI CATG 9 cut(s) 422, 620, 790, 804, 839, 976, 1042, 1345, 1414
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 1109, 1141
FatI CATG 9 cut(s) 418, 616, 786, 800, 835, 972, 1038, 1341, 1410
FblI GTMKAC 1 cut(s) 999
Fnu4HI GCNGC 3 cut(s) 65, 1008, 1140
FokI GGATG 2 cut(s) 401, 620
FriOI GRGCYC 2 cut(s) 8, 89
Fsp4HI GCNGC 3 cut(s) 65, 1008, 1140
FspAI RTGCGCAY 1 cut(s) 587
FspBI CTAG 1 cut(s) 1131
FspI TGCGCA 1 cut(s) 587
GlaI GCGC 2 cut(s) 587, 1229
GluI GCNGC 3 cut(s) 65, 1008, 1140
GsuI CTGGAG 1 cut(s) 886
HaeII RGCGCY 1 cut(s) 1231
HaeIII GGCC 5 cut(s) 112, 125, 904, 1122, 1221
HapII CCGG 1 cut(s) 68
HhaI GCGC 2 cut(s) 588, 1230
Hin1II CATG 9 cut(s) 422, 620, 790, 804, 839, 976, 1042, 1345, 1414
Hin6I GCGC 2 cut(s) 586, 1228
HinP1I GCGC 2 cut(s) 586, 1228
HinfI GANTC 3 cut(s) 318, 340, 374
HpaII CCGG 1 cut(s) 68
HphI GGTGA 1 cut(s) 1174
Hpy166II GTNNAC 1 cut(s) 1000
Hpy188I TCNGA 7 cut(s) 102, 216, 339, 428, 1099, 1310, 1349
Hpy188III TCNNGA 6 cut(s) 458, 559, 637, 854, 880, 1145
Hpy8I GTNNAC 1 cut(s) 1000
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 5 cut(s) 636, 740, 879, 1357, 1427
HpyCH4III ACNGT 8 cut(s) 78, 160, 266, 743, 1156, 1287, 1355, 1457
HpyCH4IV ACGT 2 cut(s) 39, 435
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 64, 118, 648, 1227, 1270, 1333, 1342
HpyF3I CTNAG 8 cut(s) 90, 425, 644, 671, 965, 994, 1188, 1346
HpySE526I ACGT 2 cut(s) 39, 435
Hsp92II CATG 9 cut(s) 422, 620, 790, 804, 839, 976, 1042, 1345, 1414
HspAI GCGC 2 cut(s) 586, 1228
Kzo9I GATC 2 cut(s) 71, 715
LmnI GCTCC 4 cut(s) 84, 740, 1048, 1114
Lsp1109I GCAGC 2 cut(s) 1019, 1126
LweI GCATC 4 cut(s) 289, 638, 951, 1323
MaeI CTAG 1 cut(s) 1131
MaeII ACGT 2 cut(s) 39, 435
MaeIII GTNAC 3 cut(s) 380, 1180, 1457
MalI GATC 2 cut(s) 73, 717
MboI GATC 2 cut(s) 71, 715
MhlI GDGCHC 2 cut(s) 8, 89
MlyI GAGTC 2 cut(s) 312, 368
MseI TTAA 4 cut(s) 438, 938, 1050, 1116
MslI CAYNNNNRTG 2 cut(s) 438, 799
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 2 cut(s) 69, 906
MvaI CCWGG 1 cut(s) 906
MwoI GCNNNNNNNGC 8 cut(s) 12, 64, 118, 648, 1227, 1270, 1333, 1342
NciI CCSGG 1 cut(s) 69
NdeII GATC 2 cut(s) 71, 715
NlaIII CATG 9 cut(s) 422, 620, 790, 804, 839, 976, 1042, 1345, 1414
NlaIV GGNNCC 2 cut(s) 86, 1044
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 380, 1180
NsbI TGCGCA 1 cut(s) 587
NspI RCATGY 1 cut(s) 1345
NspV TTCGAA 1 cut(s) 1250
PaeR7I CTCGAG 1 cut(s) 852
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 3 cut(s) 66, 1009, 1141
PleI GAGTC 2 cut(s) 312, 368
PpsI GAGTC 2 cut(s) 312, 368
PpuMI RGGWCCY 1 cut(s) 553
PshAI GACNNNNGTC 1 cut(s) 378
PsiI TTATAA 1 cut(s) 1023
Psp1406I AACGTT 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 904
PspGI CCWGG 1 cut(s) 904
PspN4I GGNNCC 2 cut(s) 86, 1044
PspPI GGNCC 3 cut(s) 553, 1120, 1219
PspPPI RGGWCCY 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 80, 765, 1037
RsaNI GTAC 3 cut(s) 79, 764, 1036
RseI CAYNNNNRTG 2 cut(s) 438, 799
SaqAI TTAA 4 cut(s) 438, 938, 1050, 1116
SatI GCNGC 3 cut(s) 65, 1008, 1140
Sau3AI GATC 2 cut(s) 71, 715
Sau96I GGNCC 3 cut(s) 553, 1120, 1219
SchI GAGTC 2 cut(s) 312, 368
ScrFI CCNGG 2 cut(s) 69, 906
SduI GDGCHC 2 cut(s) 8, 89
SfaNI GCATC 4 cut(s) 289, 638, 951, 1323
SfcI CTRYAG 1 cut(s) 1443
Sfr274I CTCGAG 1 cut(s) 852
SfuI TTCGAA 1 cut(s) 1250
SinI GGWCC 1 cut(s) 553
SlaI CTCGAG 1 cut(s) 852
SmiMI CAYNNNNRTG 2 cut(s) 438, 799
SmlI CTYRAG 2 cut(s) 376, 852
SmoI CTYRAG 2 cut(s) 376, 852
SsiI CCGC 2 cut(s) 65, 475
SspMI CTAG 1 cut(s) 1131
StyD4I CCNGG 2 cut(s) 67, 904
TaaI ACNGT 8 cut(s) 78, 160, 266, 743, 1156, 1287, 1355, 1457
TaiI ACGT 2 cut(s) 42, 438
TaqI TCGA 5 cut(s) 18, 290, 718, 853, 1250
TatI WGTACW 3 cut(s) 78, 763, 1035
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 4 cut(s) 438, 938, 1050, 1116
Tru9I TTAA 4 cut(s) 438, 938, 1050, 1116
TscAI CASTG 8 cut(s) 81, 165, 675, 748, 1092, 1290, 1360, 1393
TseFI GTSAC 2 cut(s) 380, 1180
TseI GCWGC 2 cut(s) 1007, 1139
Tsp45I GTSAC 2 cut(s) 380, 1180
TspDTI ATGAA 8 cut(s) 238, 341, 403, 417, 789, 852, 1091, 1464
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 8 cut(s) 81, 165, 675, 748, 1092, 1290, 1360, 1393
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 4 cut(s) 505, 682, 875, 1017
XceI RCATGY 1 cut(s) 1345
XhoI CTCGAG 1 cut(s) 852
XmiI GTMKAC 1 cut(s) 999
XspI CTAG 1 cut(s) 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.