Rw7G033540

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004811
Physical Location & Seq
Reverse (-)
49481552 .. 49489154
7603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G033540.1

Sequence Viewer

Length: 1728 bp
ATGCACGGCTACAGCCGCCTCGGCATGCCCCGCGGCGGCGGCGGCTCCATTACCGCCTCGCCGCCGTCCTCGCCGCGGTGCCGCCACGGCCGGAGCAAGAACTCCTCCGGCGGCGGCGGCGGCGGCCGCGGCCCCAAGCAGCAGAGCATTGCGGAGAGGGCGGTGTTTTTTATCATCTCGGTGGTGTTTAGAAGACGAGGCATGCTTTTATTCGCGCCTCTGTTGTATATTTCCGGAATGTTGTTGTATATGGGCTCTGTGAGCTTCGATGTAGTTAGCTTGAAGAACGTTGTGATTATCAGTAAGCCGTCGCCGCCGGGATCGCTGTACAGGAGTCCTCAGGTTTTTCAGAAGCTCTGGCCTTATATGCAGGCCGAGAGTAATCACACTCCCAATTTGTTGATGACAGTGTGGAACTCAAATGTGCATAAAGTTTGGAAGCCTTGTATTCCCAAAGATACTCTGAAAGCAGAATTACCAAAGTCAAATGGTCTATTCATAATTGAAGCGAACGGTGGGTTGAACCAGCAACGGTTGTCGATATGTGATGCAGTTGCTGTGGCTGGACTCTTAAATGCAACTCTTCTGATTCCAATCTTTCATCTAAATAGTGTATGGAGAGACTCAAGTCACTTTGGGAACATATTTGATGAGGATTTCTTCATCCATGCTCTCAGAAATCACGTTAATGTGGTCAGGGAACTTCCAGAAGATATACTTCAGCGGTTTGACAATAATATAAGCAACATTGTGAATTTGAGGGTGAAAGGTTGGTCAAGTTCAGCTCACTATCTCCGTAAGGTCCTCCCGAAGTTAGAGGAGATGGGGGCCGTGCGCATTGCACCTTTTTCAAACAGATTGGCTCATGCGGTTCCTTCAAACGTCCAGAGCCTTAGATGCTTAACCAATTTTGAAGCACTGAGATTCTCGAATTCAATAAGACTGCTTGCAGATAAAATGGTTGATCGAATGGTCAAAAGTAGCTCCCATAGTGAAGGCAAATATGTTTCAGTACATCTTCGCTTTGAAGAGGACATGGTTGCGTTTTCATGCTGTGAATATGATGGTGGGGAGGAAGAGAAACATGAAATGGATATTGCTCGAGAAAGAAGCTGGAGAGGAAAATTCAGGAGAAGGGGTAGAGTAATTAGGCCAGGTGCAAATCGTGTTGATGGGAAATGCCCTTTAACGCCATTAGAGGTTGGAATGATGCTCAGGGGCATGGGATTTGATAATACCACCTCAGTATACATTGCAGCAGGTAAAATTTATAAAGCAGAAAAGTACATGGCTCCACTTAAACAGATGTTTCCACGCTTGCAAATGAAAGACACACTGGCTACTTCAGAAGAACTTGCTCCTTTTCAGGGCCACTTATCTAGGTTGGCTGCTCTTGATTACACGGTTTGCCTCCATAGTGAAGTGTTTGTCACCACTCAGGGGGGTAATTTTCCCCACTTTTTGATGGGCCACCGGCGCTATCTCTATGGAGGACATTCGAAGACAATCAAGCCTGATAAGCGTAAATTGGCGCTGTTGTTTGATAATCCCAGTATCAGATGGGAAGATTTCAAGCGACAGATGCAAGACATGCTCAGACACAGTGAGCAGAAGGGTGTTGAAGTGAGAAAACCCAGTGCCTCACTATATACATTTCCCATGCCAGATTGTATGTGTAAGGAAGGAGAAGCTATAGATGAAAACAGTAACACAACAACACAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

575

Amino Acids

64.77

Weight (kDa)

9.62

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 168 - 495 2.6e-99 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1272
Acc16I TGCGCA 1 cut(s) 836
Acc36I ACCTGC 1 cut(s) 1250
AccB1I GGYRCC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 1248
AccII CGCG 4 cut(s) 33, 76, 129, 215
AccIII TCCGGA 1 cut(s) 233
AclI AACGTT 1 cut(s) 288
AclWI GGATC 1 cut(s) 328
AcoI YGGCCR 2 cut(s) 88, 124
AcsI RAATTY 4 cut(s) 754, 931, 1124, 1266
AcuI CTGAAG 2 cut(s) 704, 1329
AfaI GTAC 3 cut(s) 329, 1014, 1286
AfiI CCNNNNNNNGG 4 cut(s) 35, 75, 1367, 1440
AjnI CCWGG 1 cut(s) 1153
AluBI AGCT 7 cut(s) 264, 279, 355, 785, 984, 1113, 1691
AluI AGCT 7 cut(s) 264, 279, 355, 785, 984, 1113, 1691
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 1 cut(s) 328
AlwNI CAGNNNCTG 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 1101
Aor13HI TCCGGA 1 cut(s) 233
AoxI GGCC 9 cut(s) 88, 124, 130, 359, 372, 828, 1151, 1369, 1468
ApeKI GCWGC 3 cut(s) 139, 1256, 1388
ApoI RAATTY 4 cut(s) 754, 931, 1124, 1266
AspLEI GCGC 4 cut(s) 217, 837, 1479, 1534
AspS9I GGNCC 5 cut(s) 131, 802, 828, 1369, 1468
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 775, 1423
AsuII TTCGAA 1 cut(s) 1499
AvaI CYCGRG 1 cut(s) 1101
AvaII GGWCC 1 cut(s) 802
AxyI CCTNAGG 1 cut(s) 339
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 257
BbsI GAAGAC 2 cut(s) 199, 1508
BbvI GCAGC 3 cut(s) 151, 1268, 1375
BccI CCATC 5 cut(s) 817, 1058, 1166, 1459, 1554
BceAI ACGGC 5 cut(s) 22, 49, 103, 292, 815
BciT130I CCWGG 1 cut(s) 1155
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 1 cut(s) 1380
BfmI CTRYAG 2 cut(s) 10, 1692
BfoI RGCGCY 2 cut(s) 1480, 1535
BfuAI ACCTGC 1 cut(s) 1250
BglI GCCNNNNNGGC 2 cut(s) 21, 87
Bme1390I CCNGG 2 cut(s) 318, 1155
Bme18I GGWCC 1 cut(s) 802
BmeT110I CYCGRG 1 cut(s) 1101
BmgT120I GGNCC 5 cut(s) 131, 802, 828, 1369, 1468
BmiI GGNNCC 6 cut(s) 46, 80, 133, 829, 873, 1293
BmrFI CCNGG 2 cut(s) 318, 1155
BmrI ACTGGG 2 cut(s) 1545, 1629
BmsI GCATC 4 cut(s) 538, 887, 1200, 1572
BmuI ACTGGG 2 cut(s) 1545, 1629
BoxI GACNNNNGTC 1 cut(s) 627
BpiI GAAGAC 2 cut(s) 199, 1508
BpmI CTGGAG 1 cut(s) 1135
Bpu10I CCTNAGC 1 cut(s) 1214
Bpu14I TTCGAA 1 cut(s) 1499
BpuEI CTTGAG 1 cut(s) 610
BpuMI CCSGG 1 cut(s) 318
BsaBI GATNNNNATC 1 cut(s) 593
BsaJI CCNNGG 5 cut(s) 19, 31, 74, 85, 127
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 1123, 1153
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 75, 1367, 1440
Bse118I RCCGGY 1 cut(s) 1473
Bse1I ACTGG 3 cut(s) 1341, 1551, 1635
Bse21I CCTNAGG 1 cut(s) 339
Bse3DI GCAATG 3 cut(s) 147, 837, 1251
Bse8I GATNNNNATC 1 cut(s) 593
BseAI TCCGGA 1 cut(s) 233
BseBI CCWGG 1 cut(s) 1155
BseDI CCNNGG 5 cut(s) 19, 31, 74, 85, 127
BseGI GGATG 1 cut(s) 663
BseJI GATNNNNATC 1 cut(s) 593
BseLI CCNNNNNNNGG 4 cut(s) 35, 75, 1367, 1440
BseMI GCAATG 3 cut(s) 147, 837, 1251
BseMII CTCAG 7 cut(s) 353, 688, 911, 1228, 1257, 1451, 1609
BseNI ACTGG 3 cut(s) 1341, 1551, 1635
BseRI GAGGAG 2 cut(s) 94, 833
BseX3I CGGCCG 2 cut(s) 88, 124
BseXI GCAGC 3 cut(s) 151, 1268, 1375
Bsh1236I CGCG 4 cut(s) 33, 76, 129, 215
Bsh1285I CGRYCG 2 cut(s) 91, 127
BshFI GGCC 9 cut(s) 90, 126, 132, 361, 374, 830, 1153, 1371, 1470
BshNI GGYRCC 1 cut(s) 78
BsiEI CGRYCG 2 cut(s) 91, 127
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 5 cut(s) 91, 108, 234, 317, 1474
BslI CCNNNNNNNGG 4 cut(s) 35, 75, 1367, 1440
BsmAI GTCTC 1 cut(s) 615
BsnI GGCC 9 cut(s) 90, 126, 132, 361, 374, 830, 1153, 1371, 1470
BsoBI CYCGRG 1 cut(s) 1101
Bsp119I TTCGAA 1 cut(s) 1499
Bsp1286I GDGCHC 1 cut(s) 257
Bsp13I TCCGGA 1 cut(s) 233
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 2 cut(s) 320, 964
BspANI GGCC 9 cut(s) 90, 126, 132, 361, 374, 830, 1153, 1371, 1470
BspCNI CTCAG 7 cut(s) 352, 687, 912, 1227, 1256, 1450, 1608
BspEI TCCGGA 1 cut(s) 233
BspFNI CGCG 4 cut(s) 33, 76, 129, 215
BspLI GGNNCC 6 cut(s) 46, 80, 133, 829, 873, 1293
BspMI ACCTGC 1 cut(s) 1250
BspPI GGATC 1 cut(s) 328
BspT104I TTCGAA 1 cut(s) 1499
BspT107I GGYRCC 1 cut(s) 78
BsrDI GCAATG 3 cut(s) 147, 837, 1251
BsrFI RCCGGY 1 cut(s) 1473
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 3 cut(s) 1341, 1551, 1635
BssAI RCCGGY 1 cut(s) 1473
BssECI CCNNGG 5 cut(s) 19, 31, 74, 85, 127
BssMI GATC 2 cut(s) 320, 964
BssNAI GTATAC 1 cut(s) 1249
Bst1107I GTATAC 1 cut(s) 1249
Bst2UI CCWGG 1 cut(s) 1155
Bst4CI ACNGT 6 cut(s) 409, 515, 534, 1405, 1604, 1706
Bst6I CTCTTC 3 cut(s) 588, 1023, 1071
BstAPI GCANNNNNTGC 1 cut(s) 1591
BstAUI TGTACA 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 1499
BstC8I GCNNGC 5 cut(s) 26, 203, 372, 948, 1319
BstDEI CTNAG 8 cut(s) 339, 674, 893, 920, 1214, 1243, 1437, 1595
BstDSI CCRYGG 4 cut(s) 31, 74, 85, 127
BstF5I GGATG 1 cut(s) 663
BstFNI CGCG 4 cut(s) 33, 76, 129, 215
BstH2I RGCGCY 2 cut(s) 1480, 1535
BstHHI GCGC 4 cut(s) 217, 837, 1479, 1534
BstKTI GATC 2 cut(s) 323, 967
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 2 cut(s) 320, 964
BstMCI CGRYCG 2 cut(s) 91, 127
BstNI CCWGG 1 cut(s) 1155
BstNSI RCATGY 3 cut(s) 28, 205, 1594
BstPAI GACNNNNGTC 1 cut(s) 627
BstSCI CCNGG 2 cut(s) 316, 1153
BstSFI CTRYAG 2 cut(s) 10, 1692
BstUI CGCG 4 cut(s) 33, 76, 129, 215
BstV1I GCAGC 3 cut(s) 151, 1268, 1375
BstV2I GAAGAC 2 cut(s) 199, 1508
BstZ17I GTATAC 1 cut(s) 1249
BstZI CGGCCG 2 cut(s) 88, 124
Bsu36I CCTNAGG 1 cut(s) 339
BsuRI GGCC 9 cut(s) 90, 126, 132, 361, 374, 830, 1153, 1371, 1470
BtgI CCRYGG 4 cut(s) 31, 74, 85, 127
BtsCI GGATG 1 cut(s) 663
BtsIMutI CAGTG 5 cut(s) 414, 917, 1334, 1609, 1642
BveI ACCTGC 1 cut(s) 1250
Cac8I GCNNGC 5 cut(s) 26, 203, 372, 948, 1319
CaiI CAGNNNCTG 1 cut(s) 557
CciNI GCGGCCGC 1 cut(s) 124
CfoI GCGC 4 cut(s) 217, 837, 1479, 1534
Cfr10I RCCGGY 1 cut(s) 1473
Cfr13I GGNCC 5 cut(s) 131, 802, 828, 1369, 1468
Cfr42I CCGCGG 3 cut(s) 34, 77, 130
Csp6I GTAC 3 cut(s) 328, 1013, 1285
CviQI GTAC 3 cut(s) 328, 1013, 1285
DdeI CTNAG 8 cut(s) 339, 674, 893, 920, 1214, 1243, 1437, 1595
DpnI GATC 2 cut(s) 322, 966
DpnII GATC 2 cut(s) 320, 964
EaeI YGGCCR 2 cut(s) 88, 124
EagI CGGCCG 2 cut(s) 88, 124
Eam1104I CTCTTC 3 cut(s) 588, 1023, 1071
EarI CTCTTC 3 cut(s) 588, 1023, 1071
EclXI CGGCCG 2 cut(s) 88, 124
Eco24I GRGCYC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 802
Eco52I CGGCCG 2 cut(s) 88, 124
Eco57I CTGAAG 2 cut(s) 704, 1329
Eco81I CCTNAGG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 1101
EcoO109I RGGNCCY 1 cut(s) 802
EcoRI GAATTC 1 cut(s) 931
EcoRII CCWGG 1 cut(s) 1153
EcoT38I GRGCYC 1 cut(s) 257
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FauI CCCGC 1 cut(s) 38
FblI GTMKAC 1 cut(s) 1248
FokI GGATG 1 cut(s) 650
FriOI GRGCYC 1 cut(s) 257
FspAI RTGCGCAY 1 cut(s) 836
FspBI CTAG 1 cut(s) 1380
FspI TGCGCA 1 cut(s) 836
GlaI GCGC 4 cut(s) 216, 836, 1478, 1533
GsuI CTGGAG 1 cut(s) 1135
HaeII RGCGCY 2 cut(s) 1480, 1535
HaeIII GGCC 9 cut(s) 90, 126, 132, 361, 374, 830, 1153, 1371, 1470
HapII CCGG 5 cut(s) 91, 108, 234, 317, 1474
HhaI GCGC 4 cut(s) 217, 837, 1479, 1534
Hin6I GCGC 4 cut(s) 215, 835, 1477, 1532
HinP1I GCGC 4 cut(s) 215, 835, 1477, 1532
HinfI GANTC 5 cut(s) 334, 567, 589, 623, 924
HpaII CCGG 5 cut(s) 91, 108, 234, 317, 1474
HphI GGTGA 2 cut(s) 775, 1423
Hpy166II GTNNAC 1 cut(s) 1249
Hpy188I TCNGA 7 cut(s) 351, 465, 588, 677, 1348, 1559, 1598
Hpy188III TCNNGA 8 cut(s) 234, 707, 808, 886, 928, 1103, 1129, 1394
Hpy8I GTNNAC 1 cut(s) 1249
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 5 cut(s) 885, 989, 1128, 1606, 1676
HpyCH4III ACNGT 6 cut(s) 409, 515, 534, 1405, 1604, 1706
HpyCH4IV ACGT 3 cut(s) 288, 684, 882
HpyF3I CTNAG 8 cut(s) 339, 674, 893, 920, 1214, 1243, 1437, 1595
HpySE526I ACGT 3 cut(s) 288, 684, 882
HspAI GCGC 4 cut(s) 215, 835, 1477, 1532
Kpn2I TCCGGA 1 cut(s) 233
KspI CCGCGG 3 cut(s) 34, 77, 130
Kzo9I GATC 2 cut(s) 320, 964
LmnI GCTCC 5 cut(s) 50, 93, 989, 1297, 1363
Lsp1109I GCAGC 3 cut(s) 151, 1268, 1375
LweI GCATC 4 cut(s) 538, 887, 1200, 1572
MaeI CTAG 1 cut(s) 1380
MaeII ACGT 3 cut(s) 288, 684, 882
MaeIII GTNAC 3 cut(s) 629, 1429, 1706
MalI GATC 2 cut(s) 322, 966
MboI GATC 2 cut(s) 320, 964
MhlI GDGCHC 1 cut(s) 257
MlyI GAGTC 3 cut(s) 343, 561, 617
MmeI TCCRAC 1 cut(s) 1183
MroI TCCGGA 1 cut(s) 233
MseI TTAA 5 cut(s) 572, 687, 902, 1187, 1299
MslI CAYNNNNRTG 2 cut(s) 179, 687
MspA1I CMGCKG 4 cut(s) 33, 76, 129, 724
MspI CCGG 5 cut(s) 91, 108, 234, 317, 1474
MspR9I CCNGG 2 cut(s) 318, 1155
MvaI CCWGG 1 cut(s) 1155
MvnI CGCG 4 cut(s) 33, 76, 129, 215
NciI CCSGG 1 cut(s) 318
NdeII GATC 2 cut(s) 320, 964
NlaIV GGNNCC 6 cut(s) 46, 80, 133, 829, 873, 1293
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 629, 1429
NotI GCGGCCGC 1 cut(s) 124
NsbI TGCGCA 1 cut(s) 836
NspI RCATGY 3 cut(s) 28, 205, 1594
NspV TTCGAA 1 cut(s) 1499
PaeI GCATGC 2 cut(s) 28, 205
PaeR7I CTCGAG 1 cut(s) 1101
PfeI GAWTC 2 cut(s) 589, 924
PleI GAGTC 3 cut(s) 342, 561, 617
PpsI GAGTC 3 cut(s) 342, 561, 617
PpuMI RGGWCCY 1 cut(s) 802
PshAI GACNNNNGTC 1 cut(s) 627
PsiI TTATAA 1 cut(s) 1272
Psp1406I AACGTT 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 802
Psp6I CCWGG 1 cut(s) 1153
PspGI CCWGG 1 cut(s) 1153
PspN4I GGNNCC 6 cut(s) 46, 80, 133, 829, 873, 1293
PspPI GGNCC 5 cut(s) 131, 802, 828, 1369, 1468
PspPPI RGGWCCY 1 cut(s) 802
PstNI CAGNNNCTG 1 cut(s) 557
RsaI GTAC 3 cut(s) 329, 1014, 1286
RsaNI GTAC 3 cut(s) 328, 1013, 1285
RseI CAYNNNNRTG 2 cut(s) 179, 687
SacII CCGCGG 3 cut(s) 34, 77, 130
SaqAI TTAA 5 cut(s) 572, 687, 902, 1187, 1299
Sau3AI GATC 2 cut(s) 320, 964
Sau96I GGNCC 5 cut(s) 131, 802, 828, 1369, 1468
SchI GAGTC 3 cut(s) 343, 561, 617
ScrFI CCNGG 2 cut(s) 318, 1155
SduI GDGCHC 1 cut(s) 257
SfaNI GCATC 4 cut(s) 538, 887, 1200, 1572
SfcI CTRYAG 2 cut(s) 10, 1692
Sfr274I CTCGAG 1 cut(s) 1101
Sfr303I CCGCGG 3 cut(s) 34, 77, 130
SfuI TTCGAA 1 cut(s) 1499
SgrAI CRCCGGYG 1 cut(s) 1473
SgrBI CCGCGG 3 cut(s) 34, 77, 130
SinI GGWCC 1 cut(s) 802
SlaI CTCGAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 2 cut(s) 179, 687
SmlI CTYRAG 2 cut(s) 625, 1101
SmoI CTYRAG 2 cut(s) 625, 1101
SphI GCATGC 2 cut(s) 28, 205
SspMI CTAG 1 cut(s) 1380
StyD4I CCNGG 2 cut(s) 316, 1153
TaaI ACNGT 6 cut(s) 409, 515, 534, 1405, 1604, 1706
TaiI ACGT 3 cut(s) 291, 687, 885
TaqI TCGA 6 cut(s) 267, 539, 929, 967, 1102, 1499
TatI WGTACW 3 cut(s) 327, 1012, 1284
TfiI GAWTC 2 cut(s) 589, 924
Tru1I TTAA 5 cut(s) 572, 687, 902, 1187, 1299
Tru9I TTAA 5 cut(s) 572, 687, 902, 1187, 1299
TscAI CASTG 5 cut(s) 414, 924, 1341, 1609, 1642
TseFI GTSAC 2 cut(s) 629, 1429
TseI GCWGC 3 cut(s) 139, 1256, 1388
Tsp45I GTSAC 2 cut(s) 629, 1429
TspDTI ATGAA 7 cut(s) 487, 590, 652, 1038, 1101, 1340, 1713
TspGWI ACGGA 1 cut(s) 785
TspRI CASTG 5 cut(s) 414, 924, 1341, 1609, 1642
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 4 cut(s) 754, 931, 1124, 1266
XceI RCATGY 3 cut(s) 28, 205, 1594
XhoI CTCGAG 1 cut(s) 1101
XmiI GTMKAC 1 cut(s) 1248
XspI CTAG 1 cut(s) 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.