Rroxscaffold_3G00230930

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004811
Physical Location & Seq
Forward (+)
15885606 .. 15893530
7925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00230930.1

Sequence Viewer

Length: 1800 bp
ATGCACGGCTACAGTCGCCTCGGCATGCCCCGCGGCGGTGGCGGCTCTATTAACGCCTCGCCGCCGTCCTCGCCGCGGTGCCGCCACGGCCGGAGCAAGAACTCCTCCGGAGGCGGCGGCGGCCGCGGCCCCAAGCAGCAGAGCATTGCGGAGAGGGCGGTGTTTTTTATCATCTCGGTGGTGTTTAGAAGACGAGGCATGCTTTTATTCGCGCCTCTGTTGTATATTTCTGGAATGTTGTTGTATATGGGCTCTGTGAGCTTCGATGTAGTTAGCTTGAAGAACGTTGTGATTATCAGTAAGCCGTCGCCGCCGGGATCACTGTACAGGAGTCCTCAGGTTTTTCAGAAGCTGTGGCCTTATATGCAGGCCGAGAGTAATCACACTCCCAATTTGTTGATGACAGTGTGGAACTCAAATGTGCATAAAGTTTGGAAGCCTTGTATTCCCAAAGATACTCTGAAAGCAGAATTACCAAAGTCAAATGGTCTATTCATAATTGAAGCGAACGGTGGGTTGAACCAGCAACGGTTGTCGATATGTGATGCAGTTGCTGTGGCTGGACTATTAAATGCAACTCTTCTGATTCCAATCTTTCATCTAAATAGTGTTTGGAGAGACTCAAGTTTCTTTTCTGGTCAGTTACTTGCATATGGCTATAAAACTCCAGCTTATTCTGTTACCAATAGATCGTGTCACTTTGGGAACATATTTGATGAAGATTTCTTCATCCATGCTCTCAGAAATCACGTTAATGTGGTCAGGGAACTTCCAGAAGATATACTTCAGCGGTTTGACAACAATATAAGCAACATTGTGAATTTGAGAGTGAAAGGTTGGTCAAGTTCAGCTCACTATCTCCGTAAGGTCCTCCCGAAGTTAGAGGAGATGGGGGCCGTGCGCATTGCACCTTTTTCAAACAGATTGGCTCATGCAGTTCCTTCAAACGTCCAGAGCCTTAGATGCTTAGCCAATTTTGAAGCACTGAGGTTCTCGAATTCAATAAGACTGCTTGCAGATAAAATGGTTGATCGAATGGTCAAAAGTAGCTCCCACAGTGAAGGCAAATATGTTTCAGTACATCTTCGCTTTGAAGAGGACATGGTTGCGTTTTCATGCTGTGAATATGATGGTGGGGAGGAAGAGAAACATGAAATGGATATTGCTCGAGAAAGAAGCTGGAGAGGAAAATTCAGGAGAAGGGGTAGAGTAATTAGGCCAGGTGCAAATCGTGTTGATGGGAAATGCCCCTTAACGCCATTAGAGGTAGGAATGATGCTCAGGGGCATGGGATTTGATAATACCACCTCAGTATACATTGCAGCAGGTAAAATTTATAAAGCAGAAAAGTACATGGCTCCACTTAAACAGATGTTTCCACGCTTGCAAATGAAAGACACACTGGCTACTTCAGAAGAACTTGCTCCTTTTAAGGGCCACTTATCTAGGTTGGCTGCTCTTGATTACACGGTTTGCCTCCATAGTGAAGTGTTTGTCACCACTCAAGGGGGTAATTTTCCCCACTTTTTGATGGGCCACAGGCGCTATCTCTATGGAGGACATTCGAAGACAATCAAGCCTGATAAGCGTAAATTGGCACTGTTGTTTGATAATCCCAGTATCAGATGGGAAGATTTCAAGCGACAGATGCAAGACATGCTAAGACACAGTGACCAGAAGGGTGTTGAAGTGAGAAAACCCAGTGCCTCACTATATACATTTCCCATGCCAGATTGTATGTGTAAGGAAGGAGAAGCTATAGATGAAAACAGTAACACAACAACAGACTTGAATACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

599

Amino Acids

67.45

Weight (kDa)

9.61

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 167 - 517 1.4e-96 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1338
Acc16I TGCGCA 1 cut(s) 902
Acc36I ACCTGC 1 cut(s) 1316
AccB1I GGYRCC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 1314
AccII CGCG 4 cut(s) 33, 76, 126, 212
AccIII TCCGGA 1 cut(s) 107
AclI AACGTT 1 cut(s) 285
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 2 cut(s) 88, 121
AcsI RAATTY 4 cut(s) 820, 997, 1190, 1332
AcuI CTGAAG 2 cut(s) 770, 1395
AfaI GTAC 3 cut(s) 326, 1080, 1352
AfiI CCNNNNNNNGG 4 cut(s) 35, 75, 1433, 1506
AjnI CCWGG 1 cut(s) 1219
AluBI AGCT 8 cut(s) 261, 276, 352, 671, 851, 1050, 1179, 1757
AluI AGCT 8 cut(s) 261, 276, 352, 671, 851, 1050, 1179, 1757
Alw26I GTCTC 1 cut(s) 612
AlwI GGATC 1 cut(s) 325
AlwNI CAGNNNCTG 2 cut(s) 352, 554
Ama87I CYCGRG 1 cut(s) 1167
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 9 cut(s) 88, 121, 127, 356, 369, 894, 1217, 1435, 1534
ApeKI GCWGC 3 cut(s) 136, 1322, 1454
ApoI RAATTY 4 cut(s) 820, 997, 1190, 1332
AspLEI GCGC 3 cut(s) 214, 903, 1545
AspS9I GGNCC 5 cut(s) 128, 868, 894, 1435, 1534
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 1489
AsuII TTCGAA 1 cut(s) 1565
AvaI CYCGRG 1 cut(s) 1167
AvaII GGWCC 1 cut(s) 868
AxyI CCTNAGG 1 cut(s) 336
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 254
BbsI GAAGAC 2 cut(s) 196, 1574
BbvI GCAGC 3 cut(s) 148, 1334, 1441
BccI CCATC 5 cut(s) 883, 1124, 1232, 1525, 1620
BceAI ACGGC 5 cut(s) 22, 49, 103, 289, 881
BciT130I CCWGG 1 cut(s) 1221
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 1 cut(s) 1446
BfmI CTRYAG 2 cut(s) 10, 1758
BfoI RGCGCY 1 cut(s) 1546
BfuAI ACCTGC 1 cut(s) 1316
BglI GCCNNNNNGGC 1 cut(s) 87
BlpI GCTNAGC 1 cut(s) 967
Bme1390I CCNGG 2 cut(s) 315, 1221
Bme18I GGWCC 1 cut(s) 868
BmeT110I CYCGRG 1 cut(s) 1167
BmgT120I GGNCC 5 cut(s) 128, 868, 894, 1435, 1534
BmiI GGNNCC 4 cut(s) 80, 130, 895, 1359
BmrFI CCNGG 2 cut(s) 315, 1221
BmrI ACTGGG 2 cut(s) 1611, 1695
BmsI GCATC 4 cut(s) 535, 953, 1266, 1638
BmuI ACTGGG 2 cut(s) 1611, 1695
BpiI GAAGAC 2 cut(s) 196, 1574
BpmI CTGGAG 2 cut(s) 651, 1201
Bpu10I CCTNAGC 1 cut(s) 1280
Bpu1102I GCTNAGC 1 cut(s) 967
Bpu14I TTCGAA 1 cut(s) 1565
BpuEI CTTGAG 2 cut(s) 607, 1488
BpuMI CCSGG 1 cut(s) 315
BsaAI YACGTR 1 cut(s) 1797
BsaBI GATNNNNATC 1 cut(s) 590
BsaJI CCNNGG 5 cut(s) 19, 31, 74, 85, 124
BsaWI WCCGGW 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 1189, 1219
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 75, 1433, 1506
Bse1I ACTGG 3 cut(s) 1407, 1617, 1701
Bse21I CCTNAGG 1 cut(s) 336
Bse3DI GCAATG 3 cut(s) 144, 903, 1317
Bse8I GATNNNNATC 1 cut(s) 590
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 1221
BseDI CCNNGG 5 cut(s) 19, 31, 74, 85, 124
BseGI GGATG 1 cut(s) 729
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 4 cut(s) 35, 75, 1433, 1506
BseMI GCAATG 3 cut(s) 144, 903, 1317
BseMII CTCAG 5 cut(s) 350, 754, 977, 1294, 1323
BseNI ACTGG 3 cut(s) 1407, 1617, 1701
BseRI GAGGAG 2 cut(s) 94, 899
BseX3I CGGCCG 2 cut(s) 88, 121
BseXI GCAGC 3 cut(s) 148, 1334, 1441
Bsh1236I CGCG 4 cut(s) 33, 76, 126, 212
Bsh1285I CGRYCG 2 cut(s) 91, 124
BshFI GGCC 9 cut(s) 90, 123, 129, 358, 371, 896, 1219, 1437, 1536
BshNI GGYRCC 1 cut(s) 78
BsiEI CGRYCG 2 cut(s) 91, 124
BsiHKCI CYCGRG 1 cut(s) 1167
BsiSI CCGG 3 cut(s) 91, 108, 314
BslI CCNNNNNNNGG 4 cut(s) 35, 75, 1433, 1506
BsmAI GTCTC 1 cut(s) 612
BsnI GGCC 9 cut(s) 90, 123, 129, 358, 371, 896, 1219, 1437, 1536
BsoBI CYCGRG 1 cut(s) 1167
Bsp119I TTCGAA 1 cut(s) 1565
Bsp1286I GDGCHC 1 cut(s) 254
Bsp13I TCCGGA 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 324
Bsp143I GATC 3 cut(s) 317, 689, 1030
Bsp1720I GCTNAGC 1 cut(s) 967
BspANI GGCC 9 cut(s) 90, 123, 129, 358, 371, 896, 1219, 1437, 1536
BspCNI CTCAG 5 cut(s) 349, 753, 978, 1293, 1322
BspEI TCCGGA 1 cut(s) 107
BspFNI CGCG 4 cut(s) 33, 76, 126, 212
BspLI GGNNCC 4 cut(s) 80, 130, 895, 1359
BspMI ACCTGC 1 cut(s) 1316
BspPI GGATC 1 cut(s) 325
BspT104I TTCGAA 1 cut(s) 1565
BspT107I GGYRCC 1 cut(s) 78
BsrDI GCAATG 3 cut(s) 144, 903, 1317
BsrGI TGTACA 1 cut(s) 324
BsrI ACTGG 3 cut(s) 1407, 1617, 1701
BssECI CCNNGG 5 cut(s) 19, 31, 74, 85, 124
BssMI GATC 3 cut(s) 317, 689, 1030
BssNAI GTATAC 1 cut(s) 1315
Bst1107I GTATAC 1 cut(s) 1315
Bst2UI CCWGG 1 cut(s) 1221
Bst6I CTCTTC 3 cut(s) 585, 1089, 1137
BstAPI GCANNNNNTGC 1 cut(s) 1657
BstAUI TGTACA 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 1797
BstBI TTCGAA 1 cut(s) 1565
BstC8I GCNNGC 5 cut(s) 26, 200, 369, 1014, 1385
BstDEI CTNAG 8 cut(s) 336, 740, 959, 967, 986, 1280, 1309, 1661
BstDSI CCRYGG 4 cut(s) 31, 74, 85, 124
BstF5I GGATG 1 cut(s) 729
BstFNI CGCG 4 cut(s) 33, 76, 126, 212
BstH2I RGCGCY 1 cut(s) 1546
BstHHI GCGC 3 cut(s) 214, 903, 1545
BstKTI GATC 3 cut(s) 320, 692, 1033
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 3 cut(s) 317, 689, 1030
BstMCI CGRYCG 2 cut(s) 91, 124
BstNI CCWGG 1 cut(s) 1221
BstNSI RCATGY 3 cut(s) 28, 202, 1660
BstSCI CCNGG 2 cut(s) 313, 1219
BstSFI CTRYAG 2 cut(s) 10, 1758
BstSNI TACGTA 1 cut(s) 1797
BstUI CGCG 4 cut(s) 33, 76, 126, 212
BstV1I GCAGC 3 cut(s) 148, 1334, 1441
BstV2I GAAGAC 2 cut(s) 196, 1574
BstZ17I GTATAC 1 cut(s) 1315
BstZI CGGCCG 2 cut(s) 88, 121
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 9 cut(s) 90, 123, 129, 358, 371, 896, 1219, 1437, 1536
BtgI CCRYGG 4 cut(s) 31, 74, 85, 124
BtsCI GGATG 1 cut(s) 729
BtsIMutI CAGTG 8 cut(s) 320, 411, 983, 1063, 1400, 1598, 1675, 1708
BveI ACCTGC 1 cut(s) 1316
Cac8I GCNNGC 5 cut(s) 26, 200, 369, 1014, 1385
CaiI CAGNNNCTG 2 cut(s) 352, 554
CciNI GCGGCCGC 1 cut(s) 121
CfoI GCGC 3 cut(s) 214, 903, 1545
Cfr13I GGNCC 5 cut(s) 128, 868, 894, 1435, 1534
Cfr42I CCGCGG 3 cut(s) 34, 77, 127
Csp6I GTAC 3 cut(s) 325, 1079, 1351
CviQI GTAC 3 cut(s) 325, 1079, 1351
DdeI CTNAG 8 cut(s) 336, 740, 959, 967, 986, 1280, 1309, 1661
DpnI GATC 3 cut(s) 319, 691, 1032
DpnII GATC 3 cut(s) 317, 689, 1030
EaeI YGGCCR 2 cut(s) 88, 121
EagI CGGCCG 2 cut(s) 88, 121
Eam1104I CTCTTC 3 cut(s) 585, 1089, 1137
EarI CTCTTC 3 cut(s) 585, 1089, 1137
EclXI CGGCCG 2 cut(s) 88, 121
Eco105I TACGTA 1 cut(s) 1797
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 868
Eco52I CGGCCG 2 cut(s) 88, 121
Eco57I CTGAAG 2 cut(s) 770, 1395
Eco81I CCTNAGG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 1167
EcoO109I RGGNCCY 1 cut(s) 868
EcoRI GAATTC 1 cut(s) 997
EcoRII CCWGG 1 cut(s) 1219
EcoT38I GRGCYC 1 cut(s) 254
FalI AAGNNNNNCTT 4 cut(s) 768, 800, 1424, 1456
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 652
FblI GTMKAC 1 cut(s) 1314
FokI GGATG 1 cut(s) 716
FriOI GRGCYC 1 cut(s) 254
FspAI RTGCGCAY 1 cut(s) 902
FspBI CTAG 1 cut(s) 1446
FspI TGCGCA 1 cut(s) 902
GlaI GCGC 3 cut(s) 213, 902, 1544
GsuI CTGGAG 2 cut(s) 651, 1201
HaeII RGCGCY 1 cut(s) 1546
HaeIII GGCC 9 cut(s) 90, 123, 129, 358, 371, 896, 1219, 1437, 1536
HapII CCGG 3 cut(s) 91, 108, 314
HhaI GCGC 3 cut(s) 214, 903, 1545
Hin6I GCGC 3 cut(s) 212, 901, 1543
HinP1I GCGC 3 cut(s) 212, 901, 1543
HinfI GANTC 3 cut(s) 331, 586, 620
HpaII CCGG 3 cut(s) 91, 108, 314
HphI GGTGA 1 cut(s) 1489
Hpy166II GTNNAC 1 cut(s) 1315
Hpy188I TCNGA 6 cut(s) 348, 462, 585, 743, 1414, 1625
Hpy188III TCNNGA 9 cut(s) 108, 231, 773, 874, 952, 994, 1169, 1195, 1460
Hpy8I GTNNAC 1 cut(s) 1315
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 5 cut(s) 951, 1055, 1194, 1672, 1742
HpyCH4IV ACGT 4 cut(s) 285, 750, 948, 1796
HpyF3I CTNAG 8 cut(s) 336, 740, 959, 967, 986, 1280, 1309, 1661
HpySE526I ACGT 4 cut(s) 285, 750, 948, 1796
HspAI GCGC 3 cut(s) 212, 901, 1543
Kpn2I TCCGGA 1 cut(s) 107
KspI CCGCGG 3 cut(s) 34, 77, 127
Kzo9I GATC 3 cut(s) 317, 689, 1030
LmnI GCTCC 4 cut(s) 93, 1055, 1363, 1429
Lsp1109I GCAGC 3 cut(s) 148, 1334, 1441
LweI GCATC 4 cut(s) 535, 953, 1266, 1638
MaeI CTAG 1 cut(s) 1446
MaeII ACGT 4 cut(s) 285, 750, 948, 1796
MaeIII GTNAC 6 cut(s) 642, 679, 695, 1495, 1670, 1772
MalI GATC 3 cut(s) 319, 691, 1032
MboI GATC 3 cut(s) 317, 689, 1030
MhlI GDGCHC 1 cut(s) 254
MlyI GAGTC 2 cut(s) 340, 614
MroI TCCGGA 1 cut(s) 107
MseI TTAA 6 cut(s) 51, 569, 753, 1253, 1365, 1431
MslI CAYNNNNRTG 2 cut(s) 176, 753
MspA1I CMGCKG 4 cut(s) 33, 76, 126, 790
MspI CCGG 3 cut(s) 91, 108, 314
MspR9I CCNGG 2 cut(s) 315, 1221
MvaI CCWGG 1 cut(s) 1221
MvnI CGCG 4 cut(s) 33, 76, 126, 212
NciI CCSGG 1 cut(s) 315
NdeI CATATG 1 cut(s) 652
NdeII GATC 3 cut(s) 317, 689, 1030
NlaIV GGNNCC 4 cut(s) 80, 130, 895, 1359
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 3 cut(s) 695, 1495, 1670
NotI GCGGCCGC 1 cut(s) 121
NsbI TGCGCA 1 cut(s) 902
NspI RCATGY 3 cut(s) 28, 202, 1660
NspV TTCGAA 1 cut(s) 1565
PaeI GCATGC 2 cut(s) 28, 202
PaeR7I CTCGAG 1 cut(s) 1167
PfeI GAWTC 1 cut(s) 586
PleI GAGTC 2 cut(s) 339, 614
PpsI GAGTC 2 cut(s) 339, 614
Ppu21I YACGTR 1 cut(s) 1797
PpuMI RGGWCCY 1 cut(s) 868
PsiI TTATAA 1 cut(s) 1338
Psp1406I AACGTT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 868
Psp6I CCWGG 1 cut(s) 1219
PspGI CCWGG 1 cut(s) 1219
PspN4I GGNNCC 4 cut(s) 80, 130, 895, 1359
PspPI GGNCC 5 cut(s) 128, 868, 894, 1435, 1534
PspPPI RGGWCCY 1 cut(s) 868
PstNI CAGNNNCTG 2 cut(s) 352, 554
RsaI GTAC 3 cut(s) 326, 1080, 1352
RsaNI GTAC 3 cut(s) 325, 1079, 1351
RseI CAYNNNNRTG 2 cut(s) 176, 753
SacII CCGCGG 3 cut(s) 34, 77, 127
SaqAI TTAA 6 cut(s) 51, 569, 753, 1253, 1365, 1431
Sau3AI GATC 3 cut(s) 317, 689, 1030
Sau96I GGNCC 5 cut(s) 128, 868, 894, 1435, 1534
SchI GAGTC 2 cut(s) 340, 614
ScrFI CCNGG 2 cut(s) 315, 1221
SduI GDGCHC 1 cut(s) 254
SfaNI GCATC 4 cut(s) 535, 953, 1266, 1638
SfcI CTRYAG 2 cut(s) 10, 1758
Sfr274I CTCGAG 1 cut(s) 1167
Sfr303I CCGCGG 3 cut(s) 34, 77, 127
SfuI TTCGAA 1 cut(s) 1565
SgrBI CCGCGG 3 cut(s) 34, 77, 127
SinI GGWCC 1 cut(s) 868
SlaI CTCGAG 1 cut(s) 1167
SmiMI CAYNNNNRTG 2 cut(s) 176, 753
SmlI CTYRAG 3 cut(s) 622, 1167, 1503
SmoI CTYRAG 3 cut(s) 622, 1167, 1503
SnaBI TACGTA 1 cut(s) 1797
SphI GCATGC 2 cut(s) 28, 202
SspMI CTAG 1 cut(s) 1446
StyD4I CCNGG 2 cut(s) 313, 1219
TaiI ACGT 4 cut(s) 288, 753, 951, 1799
TaqI TCGA 6 cut(s) 264, 536, 995, 1033, 1168, 1565
TatI WGTACW 3 cut(s) 324, 1078, 1350
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 6 cut(s) 51, 569, 753, 1253, 1365, 1431
Tru9I TTAA 6 cut(s) 51, 569, 753, 1253, 1365, 1431
TscAI CASTG 8 cut(s) 327, 411, 990, 1063, 1407, 1605, 1675, 1708
TseFI GTSAC 3 cut(s) 695, 1495, 1670
TseI GCWGC 3 cut(s) 136, 1322, 1454
Tsp45I GTSAC 3 cut(s) 695, 1495, 1670
TspDTI ATGAA 8 cut(s) 484, 587, 718, 732, 1104, 1167, 1406, 1779
TspGWI ACGGA 1 cut(s) 851
TspRI CASTG 8 cut(s) 327, 411, 990, 1063, 1407, 1605, 1675, 1708
VpaK11BI GGWCC 1 cut(s) 868
XapI RAATTY 4 cut(s) 820, 997, 1190, 1332
XceI RCATGY 3 cut(s) 28, 202, 1660
XhoI CTCGAG 1 cut(s) 1167
XmiI GTMKAC 1 cut(s) 1314
XspI CTAG 1 cut(s) 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.