RchiOBHm_Chr7g0228671

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004811
Physical Location & Seq
Reverse (-)
52024975 .. 52032835
7861 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20484

Sequence Viewer

Length: 1725 bp
ATGCACGGCTACAGCCGCCTCGGCATGCCCCGCGGCGGCGGCGGCTCCATTACCGCCTCGCCGCCGTCCTCGCCGCGCTGCCGCCACGGCCGGAGCAAGAACTCCTCCGGCGGCGGCGGCGGCCGCGGTCCCAAGCAGCAGAGCATTGCGGAGAGGGCGGTGTTTTTTATCATCTCGGTGGTGTTTAGAAGACGAGGCATGCTTTTATTCGCGCCTCTGTTGTATATTTCCGGAATGTTGTTGTATATGGGCTCTGTGAGCTTCGATGTAGTTAGCTTGAAGAACGTTGTGATTATCAGTAAGCCGTCGCCGCCGGGATCGCTGTACAGGAGTCCTCAGGTTTTTCAGAAGCTCTGGCCTTATATGCAGGCCGAGAGTAATCACACTCCCAATTTGTTGATGACAGTGTGGAACTCAAATGTGCATAAAGTTTGGAAGCCTTGTATTCCCAAAGATACTCTGAAAGCAGAATTACCAAAGTCAAATGGTCTATTCATAATTGAAGCGAACGGTGGGTTGAACCAGCAACGGTTGTCGATATGTGATGCAGTTGCTGTGGCTGGACTCCTAAATGCAACTCTTCTGATTCCAATCTTTCATCTAAATAGTGTTTGGAGAGACTCAAGTCACTTTGGGAACATATTTGATGAAGATTTCTTCATCCATGCTCTCAGAAATCACGTTAATGTGGTCAGGGAACTTCCAGAAGATATACTTCAGCGGTTTGACAATAATATAAGCAACATTGTGAATTTGAGAGTGAAAGGTTGGTCAAGTTCAGCTCACTATCTCCGTAAGGTCCTCCCGAAGTTAGAGGAGATGGGGGCTGTGCGCATTGCACCTTTTTCAAACAGATTGGCTCATGCAGTTCCTTCAAACATCCAGAGCCTTAGATGCTTAGCCAATTTTGAAGCACTGAGGTTCTCAAATTCAATAAGACTGCTTGCAGATAAAATGGTTGATCGAATGGTCAAAAGTAGCTCCCACAGTGAAGGCAAATATGTTTCAGTACATCTTCGCTTTGAAGAGGACATGGTTGCGTTTTCATGCTGTGAATATGATGGTGGGGAGGAAGAGAAACATGAAATGGATATTGCTCGAGAAAGAAGCTGGAGAGGAAAATTCAGGAGAAGGGGTAGAGTAATTAGGCCAGGTGCAAATCGTGTTGATGGGAAATGCCCTTTAACGCCATTAGAGGTAGGAATGATGCTCAGGGGCATGGGATTTGATAATACCACCTCAGTATACATTGCAGCAGGTAAAATTTATAAAGCAGAAAAGTACATGGCTCCACTTAAACAGATGTTTCCACACTTGCAAATGAAAGACACACTGGCTACTTCAGAAGAACTTGCTCCTTTTAAGGGCCACTTATCTAGGTTGGCTGCTCTTGATTACACGGTTTGCCTCCATAGTGAAGTGTTTGTCACCACTCAGGGGGGTAATTTTCCCCACTTTTTGATGGGCCACAGGCGCTATCTCTATGGAGGACATTCGAAGACAATCAAGCCTGATAAGCGTAAATTGGCACTGTTGTTTGATAATCCCAGTATCAGATGGGAAGATTTCAAGCGACAGATGCAAGACATGCTCAGACACAGTGAGCAGAAGGGTGTTGAAGTGAGAAAACCCAGTGCCTCACTATATACATTTCCCATGCCAGATTGTATGTGTAAGGAAGGAGAAGCTATAGATGAAAACAGTAACACAACAACACAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

574

Amino Acids

64.68

Weight (kDa)

9.61

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 167 - 494 1.1e-99 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1269
Acc16I TGCGCA 1 cut(s) 833
Acc36I ACCTGC 1 cut(s) 1247
AccI GTMKAC 1 cut(s) 1245
AccII CGCG 4 cut(s) 33, 76, 126, 212
AccIII TCCGGA 1 cut(s) 230
AclI AACGTT 1 cut(s) 285
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 2 cut(s) 88, 121
AcsI RAATTY 4 cut(s) 751, 928, 1121, 1263
AcuI CTGAAG 2 cut(s) 701, 1326
AfaI GTAC 3 cut(s) 326, 1011, 1283
AfiI CCNNNNNNNGG 3 cut(s) 35, 1364, 1437
AjnI CCWGG 1 cut(s) 1150
AluBI AGCT 7 cut(s) 261, 276, 352, 782, 981, 1110, 1688
AluI AGCT 7 cut(s) 261, 276, 352, 782, 981, 1110, 1688
Alw26I GTCTC 1 cut(s) 612
AlwI GGATC 1 cut(s) 325
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 1098
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 7 cut(s) 88, 121, 356, 369, 1148, 1366, 1465
ApeKI GCWGC 4 cut(s) 78, 136, 1253, 1385
ApoI RAATTY 4 cut(s) 751, 928, 1121, 1263
AspLEI GCGC 4 cut(s) 78, 214, 834, 1476
AspS9I GGNCC 4 cut(s) 128, 799, 1366, 1465
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 1420
AsuII TTCGAA 1 cut(s) 1496
AvaI CYCGRG 1 cut(s) 1098
AvaII GGWCC 2 cut(s) 128, 799
AxyI CCTNAGG 1 cut(s) 336
BanII GRGCYC 1 cut(s) 254
BbsI GAAGAC 2 cut(s) 196, 1505
BbvI GCAGC 4 cut(s) 65, 148, 1265, 1372
BccI CCATC 5 cut(s) 814, 1055, 1163, 1456, 1551
BceAI ACGGC 4 cut(s) 22, 49, 103, 289
BciT130I CCWGG 1 cut(s) 1152
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 1 cut(s) 1377
BfmI CTRYAG 2 cut(s) 10, 1689
BfoI RGCGCY 1 cut(s) 1477
BfuAI ACCTGC 1 cut(s) 1247
BglI GCCNNNNNGGC 2 cut(s) 21, 87
BlpI GCTNAGC 1 cut(s) 898
Bme1390I CCNGG 2 cut(s) 315, 1152
Bme18I GGWCC 2 cut(s) 128, 799
BmeT110I CYCGRG 1 cut(s) 1098
BmgT120I GGNCC 4 cut(s) 128, 799, 1366, 1465
BmiI GGNNCC 3 cut(s) 46, 130, 1290
BmrFI CCNGG 2 cut(s) 315, 1152
BmrI ACTGGG 2 cut(s) 1542, 1626
BmsI GCATC 4 cut(s) 535, 884, 1197, 1569
BmuI ACTGGG 2 cut(s) 1542, 1626
BoxI GACNNNNGTC 1 cut(s) 624
BpiI GAAGAC 2 cut(s) 196, 1505
BpmI CTGGAG 1 cut(s) 1132
Bpu10I CCTNAGC 1 cut(s) 1211
Bpu1102I GCTNAGC 1 cut(s) 898
Bpu14I TTCGAA 1 cut(s) 1496
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 590
BsaJI CCNNGG 4 cut(s) 19, 31, 85, 124
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 1120, 1150
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 1364, 1437
Bse1I ACTGG 3 cut(s) 1338, 1548, 1632
Bse21I CCTNAGG 1 cut(s) 336
Bse3DI GCAATG 3 cut(s) 144, 834, 1248
Bse8I GATNNNNATC 1 cut(s) 590
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 1 cut(s) 1152
BseDI CCNNGG 4 cut(s) 19, 31, 85, 124
BseGI GGATG 2 cut(s) 660, 879
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 3 cut(s) 35, 1364, 1437
BseMI GCAATG 3 cut(s) 144, 834, 1248
BseMII CTCAG 7 cut(s) 350, 685, 908, 1225, 1254, 1448, 1606
BseNI ACTGG 3 cut(s) 1338, 1548, 1632
BseRI GAGGAG 2 cut(s) 94, 830
BseX3I CGGCCG 2 cut(s) 88, 121
BseXI GCAGC 4 cut(s) 65, 148, 1265, 1372
Bsh1236I CGCG 4 cut(s) 33, 76, 126, 212
Bsh1285I CGRYCG 2 cut(s) 91, 124
BshFI GGCC 7 cut(s) 90, 123, 358, 371, 1150, 1368, 1467
BsiEI CGRYCG 2 cut(s) 91, 124
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 4 cut(s) 91, 108, 231, 314
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 3 cut(s) 35, 1364, 1437
BsmAI GTCTC 1 cut(s) 612
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 7 cut(s) 90, 123, 358, 371, 1150, 1368, 1467
BsoBI CYCGRG 1 cut(s) 1098
Bsp119I TTCGAA 1 cut(s) 1496
Bsp1286I GDGCHC 1 cut(s) 254
Bsp13I TCCGGA 1 cut(s) 230
Bsp1407I TGTACA 1 cut(s) 324
Bsp143I GATC 2 cut(s) 317, 961
Bsp1720I GCTNAGC 1 cut(s) 898
BspANI GGCC 7 cut(s) 90, 123, 358, 371, 1150, 1368, 1467
BspCNI CTCAG 7 cut(s) 349, 684, 909, 1224, 1253, 1447, 1605
BspEI TCCGGA 1 cut(s) 230
BspFNI CGCG 4 cut(s) 33, 76, 126, 212
BspLI GGNNCC 3 cut(s) 46, 130, 1290
BspMI ACCTGC 1 cut(s) 1247
BspPI GGATC 1 cut(s) 325
BspT104I TTCGAA 1 cut(s) 1496
BsrDI GCAATG 3 cut(s) 144, 834, 1248
BsrGI TGTACA 1 cut(s) 324
BsrI ACTGG 3 cut(s) 1338, 1548, 1632
BssECI CCNNGG 4 cut(s) 19, 31, 85, 124
BssMI GATC 2 cut(s) 317, 961
BssNAI GTATAC 1 cut(s) 1246
Bst1107I GTATAC 1 cut(s) 1246
Bst2UI CCWGG 1 cut(s) 1152
Bst4CI ACNGT 8 cut(s) 406, 512, 531, 989, 1402, 1533, 1601, 1703
Bst6I CTCTTC 3 cut(s) 585, 1020, 1068
BstAPI GCANNNNNTGC 1 cut(s) 1588
BstAUI TGTACA 1 cut(s) 324
BstBI TTCGAA 1 cut(s) 1496
BstC8I GCNNGC 4 cut(s) 26, 200, 369, 945
BstDEI CTNAG 9 cut(s) 336, 671, 890, 898, 917, 1211, 1240, 1434, 1592
BstDSI CCRYGG 3 cut(s) 31, 85, 124
BstF5I GGATG 2 cut(s) 660, 879
BstFNI CGCG 4 cut(s) 33, 76, 126, 212
BstH2I RGCGCY 1 cut(s) 1477
BstHHI GCGC 4 cut(s) 78, 214, 834, 1476
BstKTI GATC 2 cut(s) 320, 964
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 2 cut(s) 317, 961
BstMCI CGRYCG 2 cut(s) 91, 124
BstNI CCWGG 1 cut(s) 1152
BstNSI RCATGY 3 cut(s) 28, 202, 1591
BstPAI GACNNNNGTC 1 cut(s) 624
BstSCI CCNGG 2 cut(s) 313, 1150
BstSFI CTRYAG 2 cut(s) 10, 1689
BstUI CGCG 4 cut(s) 33, 76, 126, 212
BstV1I GCAGC 4 cut(s) 65, 148, 1265, 1372
BstV2I GAAGAC 2 cut(s) 196, 1505
BstZ17I GTATAC 1 cut(s) 1246
BstZI CGGCCG 2 cut(s) 88, 121
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 7 cut(s) 90, 123, 358, 371, 1150, 1368, 1467
BtgI CCRYGG 3 cut(s) 31, 85, 124
BtsCI GGATG 2 cut(s) 660, 879
BtsIMutI CAGTG 7 cut(s) 411, 914, 994, 1331, 1529, 1606, 1639
BveI ACCTGC 1 cut(s) 1247
Cac8I GCNNGC 4 cut(s) 26, 200, 369, 945
CaiI CAGNNNCTG 1 cut(s) 554
CciNI GCGGCCGC 1 cut(s) 121
CfoI GCGC 4 cut(s) 78, 214, 834, 1476
Cfr13I GGNCC 4 cut(s) 128, 799, 1366, 1465
Cfr42I CCGCGG 2 cut(s) 34, 127
Csp6I GTAC 3 cut(s) 325, 1010, 1282
CviQI GTAC 3 cut(s) 325, 1010, 1282
DdeI CTNAG 9 cut(s) 336, 671, 890, 898, 917, 1211, 1240, 1434, 1592
DpnI GATC 2 cut(s) 319, 963
DpnII GATC 2 cut(s) 317, 961
EaeI YGGCCR 2 cut(s) 88, 121
EagI CGGCCG 2 cut(s) 88, 121
Eam1104I CTCTTC 3 cut(s) 585, 1020, 1068
EarI CTCTTC 3 cut(s) 585, 1020, 1068
EclXI CGGCCG 2 cut(s) 88, 121
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 2 cut(s) 128, 799
Eco52I CGGCCG 2 cut(s) 88, 121
Eco57I CTGAAG 2 cut(s) 701, 1326
Eco81I CCTNAGG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 1098
EcoO109I RGGNCCY 1 cut(s) 799
EcoRII CCWGG 1 cut(s) 1150
EcoT38I GRGCYC 1 cut(s) 254
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 1355, 1387
FaqI GGGAC 1 cut(s) 114
FauI CCCGC 1 cut(s) 38
FblI GTMKAC 1 cut(s) 1245
FokI GGATG 2 cut(s) 647, 866
FriOI GRGCYC 1 cut(s) 254
FspAI RTGCGCAY 1 cut(s) 833
FspBI CTAG 1 cut(s) 1377
FspI TGCGCA 1 cut(s) 833
GlaI GCGC 4 cut(s) 77, 213, 833, 1475
GsuI CTGGAG 1 cut(s) 1132
HaeII RGCGCY 1 cut(s) 1477
HaeIII GGCC 7 cut(s) 90, 123, 358, 371, 1150, 1368, 1467
HapII CCGG 4 cut(s) 91, 108, 231, 314
HhaI GCGC 4 cut(s) 78, 214, 834, 1476
Hin6I GCGC 4 cut(s) 76, 212, 832, 1474
HinP1I GCGC 4 cut(s) 76, 212, 832, 1474
HinfI GANTC 4 cut(s) 331, 564, 586, 620
HpaII CCGG 4 cut(s) 91, 108, 231, 314
HphI GGTGA 1 cut(s) 1420
Hpy166II GTNNAC 1 cut(s) 1246
Hpy188I TCNGA 7 cut(s) 348, 462, 585, 674, 1345, 1556, 1595
Hpy188III TCNNGA 7 cut(s) 231, 704, 805, 883, 1100, 1126, 1391
Hpy8I GTNNAC 1 cut(s) 1246
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 5 cut(s) 882, 986, 1125, 1603, 1673
HpyCH4III ACNGT 8 cut(s) 406, 512, 531, 989, 1402, 1533, 1601, 1703
HpyCH4IV ACGT 2 cut(s) 285, 681
HpyF3I CTNAG 9 cut(s) 336, 671, 890, 898, 917, 1211, 1240, 1434, 1592
HpySE526I ACGT 2 cut(s) 285, 681
HspAI GCGC 4 cut(s) 76, 212, 832, 1474
Kpn2I TCCGGA 1 cut(s) 230
KspI CCGCGG 2 cut(s) 34, 127
Kzo9I GATC 2 cut(s) 317, 961
LmnI GCTCC 5 cut(s) 50, 93, 986, 1294, 1360
Lsp1109I GCAGC 4 cut(s) 65, 148, 1265, 1372
LweI GCATC 4 cut(s) 535, 884, 1197, 1569
MaeI CTAG 1 cut(s) 1377
MaeII ACGT 2 cut(s) 285, 681
MaeIII GTNAC 3 cut(s) 626, 1426, 1703
MalI GATC 2 cut(s) 319, 963
MboI GATC 2 cut(s) 317, 961
MhlI GDGCHC 1 cut(s) 254
MlyI GAGTC 3 cut(s) 340, 558, 614
MroI TCCGGA 1 cut(s) 230
MseI TTAA 4 cut(s) 684, 1184, 1296, 1362
MslI CAYNNNNRTG 2 cut(s) 176, 684
MspA1I CMGCKG 3 cut(s) 33, 126, 721
MspI CCGG 4 cut(s) 91, 108, 231, 314
MspR9I CCNGG 2 cut(s) 315, 1152
MvaI CCWGG 1 cut(s) 1152
MvnI CGCG 4 cut(s) 33, 76, 126, 212
NciI CCSGG 1 cut(s) 315
NdeII GATC 2 cut(s) 317, 961
NlaIV GGNNCC 3 cut(s) 46, 130, 1290
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 2 cut(s) 626, 1426
NotI GCGGCCGC 1 cut(s) 121
NsbI TGCGCA 1 cut(s) 833
NspI RCATGY 3 cut(s) 28, 202, 1591
NspV TTCGAA 1 cut(s) 1496
PaeI GCATGC 2 cut(s) 28, 202
PaeR7I CTCGAG 1 cut(s) 1098
PfeI GAWTC 1 cut(s) 586
PleI GAGTC 3 cut(s) 339, 558, 614
PpsI GAGTC 3 cut(s) 339, 558, 614
PpuMI RGGWCCY 1 cut(s) 799
PshAI GACNNNNGTC 1 cut(s) 624
PsiI TTATAA 1 cut(s) 1269
Psp1406I AACGTT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 1 cut(s) 1150
PspGI CCWGG 1 cut(s) 1150
PspN4I GGNNCC 3 cut(s) 46, 130, 1290
PspPI GGNCC 4 cut(s) 128, 799, 1366, 1465
PspPPI RGGWCCY 1 cut(s) 799
PstNI CAGNNNCTG 1 cut(s) 554
RsaI GTAC 3 cut(s) 326, 1011, 1283
RsaNI GTAC 3 cut(s) 325, 1010, 1282
RseI CAYNNNNRTG 2 cut(s) 176, 684
SacII CCGCGG 2 cut(s) 34, 127
SaqAI TTAA 4 cut(s) 684, 1184, 1296, 1362
Sau3AI GATC 2 cut(s) 317, 961
Sau96I GGNCC 4 cut(s) 128, 799, 1366, 1465
SchI GAGTC 3 cut(s) 340, 558, 614
ScrFI CCNGG 2 cut(s) 315, 1152
SduI GDGCHC 1 cut(s) 254
SfaNI GCATC 4 cut(s) 535, 884, 1197, 1569
SfcI CTRYAG 2 cut(s) 10, 1689
Sfr274I CTCGAG 1 cut(s) 1098
Sfr303I CCGCGG 2 cut(s) 34, 127
SfuI TTCGAA 1 cut(s) 1496
SgrBI CCGCGG 2 cut(s) 34, 127
SinI GGWCC 2 cut(s) 128, 799
SlaI CTCGAG 1 cut(s) 1098
SmiMI CAYNNNNRTG 2 cut(s) 176, 684
SmlI CTYRAG 2 cut(s) 622, 1098
SmoI CTYRAG 2 cut(s) 622, 1098
SphI GCATGC 2 cut(s) 28, 202
SspMI CTAG 1 cut(s) 1377
StyD4I CCNGG 2 cut(s) 313, 1150
TaaI ACNGT 8 cut(s) 406, 512, 531, 989, 1402, 1533, 1601, 1703
TaiI ACGT 2 cut(s) 288, 684
TaqI TCGA 5 cut(s) 264, 536, 964, 1099, 1496
TatI WGTACW 3 cut(s) 324, 1009, 1281
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 4 cut(s) 684, 1184, 1296, 1362
Tru9I TTAA 4 cut(s) 684, 1184, 1296, 1362
TscAI CASTG 7 cut(s) 411, 921, 994, 1338, 1536, 1606, 1639
TseFI GTSAC 2 cut(s) 626, 1426
TseI GCWGC 4 cut(s) 78, 136, 1253, 1385
Tsp45I GTSAC 2 cut(s) 626, 1426
TspDTI ATGAA 8 cut(s) 484, 587, 649, 663, 1035, 1098, 1337, 1710
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 7 cut(s) 411, 921, 994, 1338, 1536, 1606, 1639
VpaK11BI GGWCC 2 cut(s) 128, 799
XapI RAATTY 4 cut(s) 751, 928, 1121, 1263
XceI RCATGY 3 cut(s) 28, 202, 1591
XhoI CTCGAG 1 cut(s) 1098
XmiI GTMKAC 1 cut(s) 1245
XspI CTAG 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.