Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010919

Physical Location & Seq

Genomic Coordinates Reverse (-)

547396 .. 549596

Gene Structure 2201 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1007200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 882 bp

ATGGACAAGGCACCAGCAGCAACCGGCTGTTACAAGTGCGGCCGACCAGGCCACTGGTCACGTGACTGCCCTTCCTCCGCCCCTACCCCCAATTCCAATTCCAATCCTAATTCCAACCCTAACCCTAACCCCACAAATCTCAATTCCTCTTCTTATCCTTTCAAAAGCGGTACTGGTACTGGGATCGAAGGGAAGGCGAAGAAGGTGTCGGTTCCGAAGACTCGGCCGAAGCTGACGCCGGAGCTGCTTCTCTCAGACGATGGGCTCGGCTACGTCCTCCGCCACTTCCCTCGCGCCTTCAAGTATCGCGGCCGCGGACACGAGGTTAGAGATTTGGGGAATCTAATTGGGATGTACACACAATGGCATTCGCGATTGCTACCATATTACTCGTTTGATCAGTTTGTTCATAAGGTCGAACAAGTTGCCGCCACCAGACGTGTGAAGATGACTCTTAGGGATTTGAGAGAAAGAGTTGCAAGTGGAGGAGACCCGACAAAGTTGCGTGAAACAGCGGTCGAGGAAGGCGTTCCAAATGATCAAGAGGAAACCTTGAACCCTGAGGGACCAAGTGATCACCAGGGAGGTTCGTCTTCAGGGAACCATGATGTGGATGATCTGCAGGAAGAGATGCTTCACGAAATCTACGAGAAAGCCACTCAGGAACCGTCTGAGACGTTCCATGGTGAGATGGTTGCTCCTAGTGCTGGTATACCTGCATCAGAAAGTTTCCAGAAACAAATAACCAATCAAGTCGAAAACAATGAAGGTAAGGAATCCAGTGAAAATCATATGACAGATGAACAGAAAGGGCGAATGGAAGCTAATAGATTGAAGGCACTTAAGAAAGCCGCAGCCCGCCGCCAATTGCAGGCAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0019219 GO:0019222 GO:0022402 GO:0031298 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033262 GO:0033554 GO:0034641 GO:0034645 GO:0042127 GO:0043111 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044773 GO:0044774 GO:0045005 GO:0045786 GO:0045930 GO:0045934 GO:0046483 GO:0048478 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:1901360 GO:1901363 GO:1901576 GO:1903047 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Swi3 zf-CCHC

Protein Analysis

294

Amino Acids

32.4

Weight (kDa)

6.81

Isoelectric Point (pI)

43.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CCHC	PF00098	9 - 25	3.5e-08	Zinc knuckle
Swi3	PF07962	77 - 158	3.4e-25	Replication Fork Protection Component Swi3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010919)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02820 AT3G02820
fragaria_vesca	FvH4_4g36440 FvH4_4g36440
malus_domestica	MD04G1110600.v1.1 MD13G1009200.v1.1 MD16G1007200.v1.1
prunus_persica	Prupe.1G343500_v2.0.a1
pyrus_communis	pycom13g00780 pycom16g00680
rosa_chinensis	RchiOBHm_Chr4g0446201
rosa_laevigata	RLG00000005683
rosa_multiflora	Rmu_sc0002266.1_g000014
rosa_roxburghii	Rroxscaffold_5G00386600
rosa_rugosa	Rorug04G0368400
rosa_samantha	Rh4AG430400 Rh4BG439800 Rh4CG457100 Rh4DG438300
rosa_wichuraiana	Rw4G036760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	724
AccB1I	GGYRCC	1 cut(s)	10
AccB7I	CCANNNNNTGG	1 cut(s)	610
AccI	GTMKAC	1 cut(s)	712
AccII	CGCG	4 cut(s)	294, 309, 315, 373
AclWI	GGATC	1 cut(s)	191
AcoI	YGGCCR	3 cut(s)	40, 224, 310
AcuI	CTGAAG	1 cut(s)	579
AcvI	CACGTG	1 cut(s)	62
AcyI	GRCGYC	1 cut(s)	236
AfaI	GTAC	3 cut(s)	172, 178, 356
AfiI	CCNNNNNNNGG	3 cut(s)	610, 707, 871
AflII	CTTAAG	1 cut(s)	842
AflIII	ACRYGT	1 cut(s)	439
AgsI	TTSAA	4 cut(s)	163, 301, 556, 835
AjiI	CACGTC	1 cut(s)	440
AjnI	CCWGG	2 cut(s)	46, 579
AluBI	AGCT	4 cut(s)	232, 244, 824, 878
AluI	AGCT	4 cut(s)	232, 244, 824, 878
Alw26I	GTCTC	2 cut(s)	483, 668
AlwI	GGATC	1 cut(s)	191
AoxI	GGCC	4 cut(s)	40, 49, 224, 310
ApeKI	GCWGC	4 cut(s)	17, 244, 854, 875
ArsI	GACNNNNNNTTYG	2 cut(s)	191, 223
Asp700I	GAANNNNTTC	1 cut(s)	528
AspLEI	GCGC	1 cut(s)	296
AspS9I	GGNCC	1 cut(s)	566
AsuHPI	GGTGA	2 cut(s)	569, 698
AvaII	GGWCC	1 cut(s)	566
AxyI	CCTNAGG	1 cut(s)	561
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	1 cut(s)	267
BauI	CACGAG	1 cut(s)	320
BbrPI	CACGTG	1 cut(s)	62
BbsI	GAAGAC	2 cut(s)	224, 585
BbvI	GCAGC	3 cut(s)	29, 231, 866
BccI	CCATC	2 cut(s)	254, 685
BcgI	CGANNNNNNTGC	4 cut(s)	407, 441, 484, 518
BciT130I	CCWGG	2 cut(s)	48, 581
BclI	TGATCA	3 cut(s)	397, 538, 574
BcoDI	GTCTC	2 cut(s)	483, 668
BfaI	CTAG	1 cut(s)	702
BfmI	CTRYAG	1 cut(s)	620
BfrI	CTTAAG	1 cut(s)	842
BfuAI	ACCTGC	1 cut(s)	724
BglI	GCCNNNNNGGC	1 cut(s)	48
Bme1390I	CCNGG	2 cut(s)	48, 581
Bme18I	GGWCC	1 cut(s)	566
BmgBI	CACGTC	1 cut(s)	440
BmgT120I	GGNCC	1 cut(s)	566
BmiI	GGNNCC	5 cut(s)	12, 213, 567, 602, 666
BmrFI	CCNGG	2 cut(s)	48, 581
BmrI	ACTGGG	1 cut(s)	189
BmsI	GCATC	2 cut(s)	621, 728
BmuI	ACTGGG	1 cut(s)	189
BpiI	GAAGAC	2 cut(s)	224, 585
BsaAI	YACGTR	1 cut(s)	62
BsaHI	GRCGYC	1 cut(s)	236
BsaI	GGTCTC	1 cut(s)	483
BsaJI	CCNNGG	3 cut(s)	313, 580, 682
Bsc4I	CCNNNNNNNGG	3 cut(s)	610, 707, 871
Bse118I	RCCGGY	1 cut(s)	23
Bse1I	ACTGG	4 cut(s)	59, 178, 184, 780
Bse21I	CCTNAGG	1 cut(s)	561
BseBI	CCWGG	2 cut(s)	48, 581
BseDI	CCNNGG	3 cut(s)	313, 580, 682
BseGI	GGATG	2 cut(s)	357, 619
BseLI	CCNNNNNNNGG	3 cut(s)	610, 707, 871
BseMII	CTCAG	4 cut(s)	267, 552, 663, 674
BseNI	ACTGG	4 cut(s)	59, 178, 184, 780
BseRI	GAGGAG	1 cut(s)	501
BseX3I	CGGCCG	3 cut(s)	40, 224, 310
BseXI	GCAGC	3 cut(s)	29, 231, 866
Bsh1236I	CGCG	4 cut(s)	294, 309, 315, 373
Bsh1285I	CGRYCG	4 cut(s)	43, 227, 313, 519
BshFI	GGCC	4 cut(s)	42, 51, 226, 312
BshNI	GGYRCC	1 cut(s)	10
BsiEI	CGRYCG	4 cut(s)	43, 227, 313, 519
BsiSI	CCGG	2 cut(s)	24, 239
BslFI	GGGAC	1 cut(s)	579
BslI	CCNNNNNNNGG	3 cut(s)	610, 707, 871
BsmAI	GTCTC	2 cut(s)	483, 668
BsmBI	CGTCTC	1 cut(s)	668
BsmFI	GGGAC	1 cut(s)	579
BsmI	GAATGC	1 cut(s)	367
BsnI	GGCC	4 cut(s)	42, 51, 226, 312
Bso31I	GGTCTC	1 cut(s)	483
Bsp1286I	GDGCHC	1 cut(s)	267
Bsp1407I	TGTACA	1 cut(s)	354
Bsp143I	GATC	5 cut(s)	183, 397, 538, 574, 616
Bsp19I	CCATGG	1 cut(s)	682
Bsp68I	TCGCGA	1 cut(s)	373
BspANI	GGCC	4 cut(s)	42, 51, 226, 312
BspCNI	CTCAG	4 cut(s)	266, 553, 664, 673
BspFNI	CGCG	4 cut(s)	294, 309, 315, 373
BspLI	GGNNCC	5 cut(s)	12, 213, 567, 602, 666
BspMAI	CTGCAG	1 cut(s)	624
BspMI	ACCTGC	1 cut(s)	724
BspPI	GGATC	1 cut(s)	191
BspT107I	GGYRCC	1 cut(s)	10
BspTI	CTTAAG	1 cut(s)	842
BspTNI	GGTCTC	1 cut(s)	483
BsrFI	RCCGGY	1 cut(s)	23
BsrGI	TGTACA	1 cut(s)	354
BsrI	ACTGG	4 cut(s)	59, 178, 184, 780
BssAI	RCCGGY	1 cut(s)	23
BssECI	CCNNGG	3 cut(s)	313, 580, 682
BssMI	GATC	5 cut(s)	183, 397, 538, 574, 616
BssNAI	GTATAC	1 cut(s)	713
BssNI	GRCGYC	1 cut(s)	236
BssSI	CACGAG	1 cut(s)	320
BssT1I	CCWWGG	1 cut(s)	682
Bst1107I	GTATAC	1 cut(s)	713
Bst2BI	CACGAG	1 cut(s)	320
Bst2UI	CCWGG	2 cut(s)	48, 581
Bst4CI	ACNGT	1 cut(s)	669
Bst6I	CTCTTC	2 cut(s)	154, 621
BstACI	GRCGYC	1 cut(s)	236
BstAFI	CTTAAG	1 cut(s)	842
BstAUI	TGTACA	1 cut(s)	354
BstBAI	YACGTR	1 cut(s)	62
BstC8I	GCNNGC	2 cut(s)	859, 873
BstDEI	CTNAG	5 cut(s)	253, 455, 561, 660, 672
BstDSI	CCRYGG	2 cut(s)	313, 682
BstF5I	GGATG	2 cut(s)	357, 619
BstFNI	CGCG	4 cut(s)	294, 309, 315, 373
BstHHI	GCGC	1 cut(s)	296
BstKTI	GATC	5 cut(s)	186, 400, 541, 577, 619
BstMAI	GTCTC	2 cut(s)	483, 668
BstMBI	GATC	5 cut(s)	183, 397, 538, 574, 616
BstMCI	CGRYCG	4 cut(s)	43, 227, 313, 519
BstMWI	GCNNNNNNNGC	4 cut(s)	17, 48, 244, 704
BstNI	CCWGG	2 cut(s)	48, 581
BstSCI	CCNGG	2 cut(s)	46, 579
BstSFI	CTRYAG	1 cut(s)	620
BstUI	CGCG	4 cut(s)	294, 309, 315, 373
BstV1I	GCAGC	3 cut(s)	29, 231, 866
BstV2I	GAAGAC	2 cut(s)	224, 585
BstXI	CCANNNNNNTGG	1 cut(s)	54
BstZ17I	GTATAC	1 cut(s)	713
BstZI	CGGCCG	3 cut(s)	40, 224, 310
Bsu36I	CCTNAGG	1 cut(s)	561
BsuRI	GGCC	4 cut(s)	42, 51, 226, 312
BtgI	CCRYGG	2 cut(s)	313, 682
BtrI	CACGTC	1 cut(s)	440
BtsCI	GGATG	2 cut(s)	357, 619
BtsIMutI	CAGTG	2 cut(s)	52, 787
BtuMI	TCGCGA	1 cut(s)	373
BveI	ACCTGC	1 cut(s)	724
Cac8I	GCNNGC	2 cut(s)	859, 873
CciNI	GCGGCCGC	1 cut(s)	310
CfoI	GCGC	1 cut(s)	296
Cfr10I	RCCGGY	1 cut(s)	23
Cfr13I	GGNCC	1 cut(s)	566
Cfr42I	CCGCGG	1 cut(s)	316
CseI	GACGC	1 cut(s)	244
Csp6I	GTAC	3 cut(s)	171, 177, 355
CviAII	CATG	2 cut(s)	605, 683
CviQI	GTAC	3 cut(s)	171, 177, 355
DdeI	CTNAG	5 cut(s)	253, 455, 561, 660, 672
DpnI	GATC	5 cut(s)	185, 399, 540, 576, 618
DpnII	GATC	5 cut(s)	183, 397, 538, 574, 616
EaeI	YGGCCR	3 cut(s)	40, 224, 310
EagI	CGGCCG	3 cut(s)	40, 224, 310
Eam1104I	CTCTTC	2 cut(s)	154, 621
EarI	CTCTTC	2 cut(s)	154, 621
EciI	GGCGGA	2 cut(s)	67, 269
EclXI	CGGCCG	3 cut(s)	40, 224, 310
Eco130I	CCWWGG	1 cut(s)	682
Eco24I	GRGCYC	1 cut(s)	267
Eco31I	GGTCTC	1 cut(s)	483
Eco47I	GGWCC	1 cut(s)	566
Eco52I	CGGCCG	3 cut(s)	40, 224, 310
Eco57I	CTGAAG	1 cut(s)	579
Eco72I	CACGTG	1 cut(s)	62
Eco81I	CCTNAGG	1 cut(s)	561
EcoRII	CCWGG	2 cut(s)	46, 579
EcoT14I	CCWWGG	1 cut(s)	682
EcoT38I	GRGCYC	1 cut(s)	267
ErhI	CCWWGG	1 cut(s)	682
Esp3I	CGTCTC	1 cut(s)	668
FaeI	CATG	2 cut(s)	608, 686
FaiI	YATR	7 cut(s)	385, 411, 606, 684, 713, 792, 794
FalI	AAGNNNNNCTT	2 cut(s)	618, 650
FaqI	GGGAC	1 cut(s)	579
FatI	CATG	2 cut(s)	604, 682
FauI	CCCGC	1 cut(s)	866
FauNDI	CATATG	1 cut(s)	792
FbaI	TGATCA	3 cut(s)	397, 538, 574
FblI	GTMKAC	1 cut(s)	712
FokI	GGATG	2 cut(s)	364, 626
FriOI	GRGCYC	1 cut(s)	267
FspBI	CTAG	1 cut(s)	702
GlaI	GCGC	1 cut(s)	295
HaeIII	GGCC	4 cut(s)	42, 51, 226, 312
HapII	CCGG	2 cut(s)	24, 239
HgaI	GACGC	1 cut(s)	244
HhaI	GCGC	1 cut(s)	296
Hin1I	GRCGYC	1 cut(s)	236
Hin1II	CATG	2 cut(s)	608, 686
Hin6I	GCGC	1 cut(s)	294
HinP1I	GCGC	1 cut(s)	294
HinfI	GANTC	4 cut(s)	220, 340, 451, 776
HpaII	CCGG	2 cut(s)	24, 239
HphI	GGTGA	2 cut(s)	569, 698
Hpy166II	GTNNAC	2 cut(s)	357, 713
Hpy188I	TCNGA	4 cut(s)	216, 256, 673, 724
Hpy188III	TCNNGA	5 cut(s)	372, 542, 638, 662, 733
Hpy8I	GTNNAC	2 cut(s)	357, 713
HpyAV	CCTTC	8 cut(s)	81, 182, 187, 196, 307, 518, 761, 829
HpyCH4III	ACNGT	1 cut(s)	669
HpyCH4IV	ACGT	4 cut(s)	61, 273, 439, 677
HpyCH4V	TGCA	4 cut(s)	479, 622, 719, 871
HpyF10VI	GCNNNNNNNGC	4 cut(s)	17, 48, 244, 704
HpyF3I	CTNAG	5 cut(s)	253, 455, 561, 660, 672
HpySE526I	ACGT	4 cut(s)	61, 273, 439, 677
Hsp92I	GRCGYC	1 cut(s)	236
Hsp92II	CATG	2 cut(s)	608, 686
HspAI	GCGC	1 cut(s)	294
Ksp22I	TGATCA	3 cut(s)	397, 538, 574
KspI	CCGCGG	1 cut(s)	316
Kzo9I	GATC	5 cut(s)	183, 397, 538, 574, 616
LmnI	GCTCC	2 cut(s)	241, 703
Lsp1109I	GCAGC	3 cut(s)	29, 231, 866
LweI	GCATC	2 cut(s)	621, 728
MaeI	CTAG	1 cut(s)	702
MaeII	ACGT	4 cut(s)	61, 273, 439, 677
MaeIII	GTNAC	3 cut(s)	29, 57, 62
MalI	GATC	5 cut(s)	185, 399, 540, 576, 618
MboI	GATC	5 cut(s)	183, 397, 538, 574, 616
MboII	GAAGA	6 cut(s)	141, 211, 229, 457, 585, 638
MfeI	CAATTG	1 cut(s)	866
MhlI	GDGCHC	1 cut(s)	267
MluCI	AATT	6 cut(s)	91, 97, 109, 142, 345, 866
MlyI	GAGTC	2 cut(s)	214, 445
MmeI	TCCRAC	1 cut(s)	138
MroXI	GAANNNNTTC	1 cut(s)	528
MseI	TTAA	1 cut(s)	843
MspA1I	CMGCKG	2 cut(s)	315, 515
MspCI	CTTAAG	1 cut(s)	842
MspI	CCGG	2 cut(s)	24, 239
MspR9I	CCNGG	2 cut(s)	48, 581
MunI	CAATTG	1 cut(s)	866
Mva1269I	GAATGC	1 cut(s)	367
MvaI	CCWGG	2 cut(s)	48, 581
MvnI	CGCG	4 cut(s)	294, 309, 315, 373
MwoI	GCNNNNNNNGC	4 cut(s)	17, 48, 244, 704
NcoI	CCATGG	1 cut(s)	682
NdeI	CATATG	1 cut(s)	792
NdeII	GATC	5 cut(s)	183, 397, 538, 574, 616
NlaIII	CATG	2 cut(s)	608, 686
NlaIV	GGNNCC	5 cut(s)	12, 213, 567, 602, 666
NmeAIII	GCCGAG	2 cut(s)	202, 246
NmuCI	GTSAC	2 cut(s)	57, 62
NotI	GCGGCCGC	1 cut(s)	310
NruI	TCGCGA	1 cut(s)	373
PcsI	WCGNNNNNNNCGW	2 cut(s)	525, 645
PctI	GAATGC	1 cut(s)	367
PdmI	GAANNNNTTC	1 cut(s)	528
PfeI	GAWTC	2 cut(s)	340, 776
PflMI	CCANNNNNTGG	1 cut(s)	610
PleI	GAGTC	2 cut(s)	214, 445
PmaCI	CACGTG	1 cut(s)	62
PmlI	CACGTG	1 cut(s)	62
PpsI	GAGTC	2 cut(s)	214, 445
Ppu21I	YACGTR	1 cut(s)	62
Psp6I	CCWGG	2 cut(s)	46, 579
PspCI	CACGTG	1 cut(s)	62
PspGI	CCWGG	2 cut(s)	46, 579
PspN4I	GGNNCC	5 cut(s)	12, 213, 567, 602, 666
PspPI	GGNCC	1 cut(s)	566
PstI	CTGCAG	1 cut(s)	624
RruI	TCGCGA	1 cut(s)	373
RsaI	GTAC	3 cut(s)	172, 178, 356
RsaNI	GTAC	3 cut(s)	171, 177, 355
SacII	CCGCGG	1 cut(s)	316
SaqAI	TTAA	1 cut(s)	843
Sau3AI	GATC	5 cut(s)	183, 397, 538, 574, 616
Sau96I	GGNCC	1 cut(s)	566
SchI	GAGTC	2 cut(s)	214, 445
ScrFI	CCNGG	2 cut(s)	48, 581
SduI	GDGCHC	1 cut(s)	267
SfaNI	GCATC	2 cut(s)	621, 728
SfcI	CTRYAG	1 cut(s)	620
SfiI	GGCCNNNNNGGCC	1 cut(s)	48
Sfr303I	CCGCGG	1 cut(s)	316
SgrBI	CCGCGG	1 cut(s)	316
SinI	GGWCC	1 cut(s)	566
SmlI	CTYRAG	1 cut(s)	842
SmoI	CTYRAG	1 cut(s)	842
Sse9I	AATT	6 cut(s)	91, 97, 109, 142, 345, 866
SspMI	CTAG	1 cut(s)	702
StyD4I	CCNGG	2 cut(s)	46, 579
StyI	CCWWGG	1 cut(s)	682
TaaI	ACNGT	1 cut(s)	669
TaiI	ACGT	4 cut(s)	64, 276, 442, 680
TaqI	TCGA	4 cut(s)	186, 417, 519, 756
TasI	AATT	6 cut(s)	91, 97, 109, 142, 345, 866
TatI	WGTACW	1 cut(s)	354
TauI	GCSGC	6 cut(s)	42, 312, 315, 431, 854, 864
TfiI	GAWTC	2 cut(s)	340, 776
Tru1I	TTAA	1 cut(s)	843
Tru9I	TTAA	1 cut(s)	843
TscAI	CASTG	2 cut(s)	59, 787
TseFI	GTSAC	2 cut(s)	57, 62
TseI	GCWGC	4 cut(s)	17, 244, 854, 875
Tsp45I	GTSAC	2 cut(s)	57, 62
TspDTI	ATGAA	3 cut(s)	398, 780, 816
TspRI	CASTG	2 cut(s)	59, 787
Van91I	CCANNNNNTGG	1 cut(s)	610
Vha464I	CTTAAG	1 cut(s)	842
VpaK11BI	GGWCC	1 cut(s)	566
XmiI	GTMKAC	1 cut(s)	712
XmnI	GAANNNNTTC	1 cut(s)	528
XspI	CTAG	1 cut(s)	702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.