MD16G1023300.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011800
Physical Location & Seq
Forward (+)
1699006 .. 1700925
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1023300.v1.1.491

Sequence Viewer

Length: 1920 bp
ATGAAAAGGGAGCACCAGACTCATCAGCTGAATCAGTACCCCCCGCCGCAGTATCCAGACCCGTCCATGGCTTCCACCTCCGCCGGCGGCGGCGGCGGCTTCTGCAAGGCCAAGATGTGGGAAGACGAATCCCAGCGAATCGACGGCGGGATGGACGAGCTTCTGGCGGTGTTGGGGTACAAGGTTCGGTCTTCGGACATGGCGGAGGTTGCTCAGAAGCTTGAGCAGCTTGAAGAATTCATGGGTTGCGCTCAGGAAGATGGACTCTCTCAGCTCGCTTCTGACACCGTTCACTATAACCCGGCGGATCTGTCGACGTGGCTTGAGAGCATGATCTCCGAGATAAACCTCCCGCCTCCTAATTTCGATCCTTTAACGGGCGGTGCCGTCGCTGGGATGCAGCCTAATCAGCAGCAAGTGCAGCTCGTCGACGATCCGTTTTTAGCTCGGGGGGAGTCGTCCATCACCACCGTCGATTTCCCAGATCAGCGGAAGAGCAAATCCATTAGCACATCACCTCCCCAAACTGTATTCGAAGACTGCAACTCTTCTTCCAGCTACTACGACTTCAAATCTATCCCCGGAAACGCCGTGTTCACCCAAACCCGCCTCGATTCTCCGTCGCGGGAGCTCAAGCGTCTCAAATCCTCATCCGGGTCTTCTCCCTCCGAGCTCCTCTTCAACCGCCCCGCCGCCTCCTCCCTTCCACAACCACAACAACCCATCTCCCTTCCCGCCACCGCCGAGTCATCACCAACCCGCCCAGCCCTCATTGTCGATTCGCAGGAAAACGGAGTCCGACTCGTCCACGGACTCATGGCCTGCGCGGAAGCCGTCCAGCAGAACAACTTCAACCTCGCCAAAGCTCTGGTGACCCAGATCGGCTACCTGGCAGGTTCACAAGCAGGTGCCATGCGTAAAGTTGCCACCTTCTTCGCCGAAGCTCTGGCCCAACGAATCTTCCGAGTCTACCCGCAGTCGCCGATCGACCACTCCTTCTCAGACATGCTGCAGATGCACTTCTACGAGACCTGCCCCTACCTGAAATTCGCCCACTTCACCGCCAATCAAGCCATCCTCGAATCCCTCCAGGGCAAAACCAGAGTCCACGTCATCGACTTCTCGATGAACCAGGGGATGCAGTGGCCCGCTCTTATGCAGGCGTTGGCGCTCAGACCCGGCGGCCCGCCCGCTTTTCGGCTCACGGGCATCGGGCCGCCGGCTTCGGATAACTCCGACCATCTGCAGGAGGTGGGTTGGAAGCTCGCCCAATTGGCTGAAACCATCCACGTCGAGTTTGAATACAGAGGATTTGTGGCAAACAGCTTGGCCGATCTTGACGCGTCGATGCTTGAACTCGGACCGAGTGAGGTTGAGTCGGTGGCGGTCAACTCGGTCTTCGAGTTGCACAAGCTGCTGGCCCGACCCGGCGCGATTGAGAAGGTGTTATCAGTGGTGAAGCAAATGAAGCCGGAGATTGTGACCGTGGTGGAGCAGGAGGCGAACCACAACGGTCCAGTGTTCATGGACCGGTTCAACGAGTCGCTCCACTATTACTCGACCCTGTTTGACTCGCTGGAGGGATCGGCGAACAGTCGGGATAAGGTGATGTCGGAAGTGTACTTGGGGAAGCAAATCTGCAACGTGGTGGCGTGCGAAGGGGTGGACCGGGTCGAGAGGCACGAGACGTTGGCCCAGTGGCGAGCCCGGTTCGGCTCGGCAGATTTCGTCCCGGTTCATCTCGGTTCGAACGCGTTCAAGCAAGCGAGTATGCTGCTGGCGTTGTTCGCCGGCGGAGATGGGTACCGGGTGGAGGAGAACGACGGGTGTATGATGTTGGCCTGGCACACTCGCCCGCTCATCGCCACCTCGGCTTGGAAACCCGCTTATAACTCGGTCATGGCTCACCGAGTCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000910 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006808 GO:0006810 GO:0006950 GO:0006970 GO:0006972 GO:0007049 GO:0007154 GO:0007165 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009739 GO:0009740 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009889 GO:0009892 GO:0009893 GO:0009894 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010187 GO:0010218 GO:0010232 GO:0010233 GO:0010453 GO:0010454 GO:0010468 GO:0010476 GO:0010556 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010648 GO:0014070 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031326 GO:0032501 GO:0032870 GO:0033206 GO:0033993 GO:0035690 GO:0040008 GO:0042176 GO:0042221 GO:0042493 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0045926 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048638 GO:0048640 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051246 GO:0051252 GO:0051301 GO:0051321 GO:0051716 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071370 GO:0071395 GO:0071396 GO:0071407 GO:0071446 GO:0071495 GO:0080050 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1900032 GO:1900033 GO:1900140 GO:1901363 GO:1901700 GO:1901701 GO:1903046 GO:1903506 GO:1905613 GO:1905614 GO:1905622 GO:2000024 GO:2000026 GO:2000033 GO:2000034 GO:2000112 GO:2000241 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
DELLA GRAS

Protein Analysis

640

Amino Acids

70.23

Weight (kDa)

5.26

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DELLA PF12041 52 - 119 3.1e-35 Transcriptional regulator DELLA protein N terminal
GRAS PF03514 268 - 627 8.2e-133 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011800)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14920 AT2G01570
fragaria_vesca FvH4_4g34110
malus_domestica MD13G1022100.v1.1 MD16G1023300.v1.1
prunus_persica Prupe.1G329600_v2.0.a1
pyrus_communis pycom13g02070 pycom16g02040 pycom16g02050
rosa_chinensis RchiOBHm_Chr4g0443621
rosa_laevigata RLG00000005909
rosa_multiflora Rmu_sc0001118.1_g000014
rosa_roxburghii Rroxscaffold_5G00384170
rosa_samantha Rh4AG403600 Rh4AG405600 Rh4BG416600 Rh4CG431100 Rh4DG412200
rosa_wichuraiana Rw4G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1890
AarI CACCTGC 1 cut(s) 896
Acc36I ACCTGC 3 cut(s) 884, 896, 1040
Acc65I GGTACC 1 cut(s) 1803
AccB1I GGYRCC 3 cut(s) 383, 908, 1803
AccB7I CCANNNNNTGG 1 cut(s) 117
AccBSI CCGCTC 2 cut(s) 1151, 1858
AccI GTMKAC 3 cut(s) 314, 429, 969
AccII CGCG 5 cut(s) 625, 827, 1343, 1433, 1754
AclWI GGATC 4 cut(s) 315, 362, 428, 1591
AcoI YGGCCR 1 cut(s) 1329
AcsI RAATTY 2 cut(s) 236, 1046
AfaI GTAC 4 cut(s) 38, 179, 1622, 1805
AfiI CCNNNNNNNGG 9 cut(s) 67, 117, 377, 393, 654, 1197, 1246, 1813, 1875
AflIII ACRYGT 2 cut(s) 1341, 1752
AgeI ACCGGT 1 cut(s) 1530
AgsI TTSAA 8 cut(s) 233, 571, 682, 853, 1301, 1355, 1537, 1759
AjiI CACGTC 3 cut(s) 318, 1111, 1291
AjnI CCWGG 4 cut(s) 888, 1089, 1131, 1841
Alw21I GWGCWC 3 cut(s) 15, 633, 675
Alw26I GTCTC 3 cut(s) 644, 1022, 1679
AlwI GGATC 4 cut(s) 315, 362, 428, 1591
Ama87I CYCGRG 1 cut(s) 447
ApeKI GCWGC 7 cut(s) 226, 400, 412, 421, 1009, 1414, 1774
ApoI RAATTY 2 cut(s) 236, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 1089, 1121
AsiGI ACCGGT 1 cut(s) 1530
Asp700I GAANNNNTTC 2 cut(s) 848, 1754
Asp718I GGTACC 1 cut(s) 1803
AspLEI GCGC 4 cut(s) 251, 827, 1171, 1433
AsuC2I CCSGG 9 cut(s) 302, 582, 655, 1179, 1428, 1670, 1708, 1733, 1808
AsuHPI GGTGA 9 cut(s) 457, 507, 589, 744, 883, 1051, 1468, 1618, 1898
AsuII TTCGAA 2 cut(s) 534, 1748
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 4 cut(s) 1361, 1514, 1528, 1666
BanI GGYRCC 3 cut(s) 383, 908, 1803
BanII GRGCYC 3 cut(s) 633, 675, 1708
BauI CACGAG 1 cut(s) 1682
BbsI GAAGAC 5 cut(s) 129, 183, 543, 651, 1390
Bbv12I GWGCWC 3 cut(s) 15, 633, 675
BbvI GCAGC 7 cut(s) 238, 412, 424, 433, 996, 1401, 1761
BccI CCATC 8 cut(s) 145, 254, 470, 731, 1082, 1248, 1292, 1793
BceAI ACGGC 4 cut(s) 160, 371, 575, 818
BcgI CGANNNNNNTGC 2 cut(s) 1756, 1790
BciT130I CCWGG 4 cut(s) 890, 1091, 1133, 1843
BciVI GTATCC 1 cut(s) 63
BcnI CCSGG 9 cut(s) 302, 582, 655, 1179, 1428, 1670, 1708, 1733, 1808
BcoDI GTCTC 3 cut(s) 644, 1022, 1679
BfmI CTRYAG 2 cut(s) 1010, 1244
BfoI RGCGCY 1 cut(s) 1172
BfuAI ACCTGC 3 cut(s) 884, 896, 1040
BfuI GTATCC 1 cut(s) 63
BglI GCCNNNNNGGC 2 cut(s) 1274, 1871
Bme18I GGWCC 4 cut(s) 1361, 1514, 1528, 1666
BmeT110I CYCGRG 1 cut(s) 447
BmgBI CACGTC 3 cut(s) 318, 1111, 1291
BmiI GGNNCC 3 cut(s) 385, 910, 1805
BmrI ACTGGG 1 cut(s) 1690
BmsI GCATC 5 cut(s) 387, 1005, 1128, 1218, 1338
BmuI ACTGGG 1 cut(s) 1690
BpiI GAAGAC 5 cut(s) 129, 183, 543, 651, 1390
BplI GAGNNNNNCTC 2 cut(s) 786, 818
BpmI CTGGAG 2 cut(s) 1073, 1598
Bpu10I CCTNAGC 1 cut(s) 252
Bpu14I TTCGAA 2 cut(s) 534, 1748
BpuEI CTTGAG 3 cut(s) 242, 344, 617
BpuMI CCSGG 9 cut(s) 302, 582, 655, 1179, 1428, 1670, 1708, 1733, 1808
BsaI GGTCTC 1 cut(s) 1022
BsaJI CCNNGG 7 cut(s) 66, 580, 808, 1090, 1132, 1485, 1869
BsaWI WCCGGW 1 cut(s) 1530
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bsc4I CCNNNNNNNGG 9 cut(s) 67, 117, 377, 393, 654, 1197, 1246, 1813, 1875
Bse118I RCCGGY 4 cut(s) 83, 1219, 1530, 1790
Bse1I ACTGG 2 cut(s) 1517, 1696
BseBI CCWGG 4 cut(s) 890, 1091, 1133, 1843
BseDI CCNNGG 7 cut(s) 66, 580, 808, 1090, 1132, 1485, 1869
BseGI GGATG 6 cut(s) 156, 402, 650, 1074, 1143, 1284
BseLI CCNNNNNNNGG 9 cut(s) 67, 117, 377, 393, 654, 1197, 1246, 1813, 1875
BseMII CTCAG 5 cut(s) 227, 266, 284, 1014, 1186
BseNI ACTGG 2 cut(s) 1517, 1696
BseRI GAGGAG 3 cut(s) 665, 688, 1829
BseXI GCAGC 7 cut(s) 238, 412, 424, 433, 996, 1401, 1761
BseYI CCCAGC 3 cut(s) 132, 392, 763
BsgI GTGCAG 1 cut(s) 440
Bsh1236I CGCG 5 cut(s) 625, 827, 1343, 1433, 1754
Bsh1285I CGRYCG 1 cut(s) 987
BshNI GGYRCC 3 cut(s) 383, 908, 1803
BshTI ACCGGT 1 cut(s) 1530
BsiEI CGRYCG 1 cut(s) 987
BsiHKAI GWGCWC 3 cut(s) 15, 633, 675
BsiHKCI CYCGRG 1 cut(s) 447
BslFI GGGAC 1 cut(s) 1715
BslI CCNNNNNNNGG 9 cut(s) 67, 117, 377, 393, 654, 1197, 1246, 1813, 1875
BsmAI GTCTC 3 cut(s) 644, 1022, 1679
BsmBI CGTCTC 2 cut(s) 644, 1679
BsmFI GGGAC 1 cut(s) 1715
Bso31I GGTCTC 1 cut(s) 1022
BsoBI CYCGRG 1 cut(s) 447
Bsp119I TTCGAA 2 cut(s) 534, 1748
Bsp1286I GDGCHC 4 cut(s) 15, 633, 675, 1708
Bsp143I GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
Bsp19I CCATGG 1 cut(s) 66
BspCNI CTCAG 5 cut(s) 226, 265, 283, 1013, 1185
BspFNI CGCG 5 cut(s) 625, 827, 1343, 1433, 1754
BspLI GGNNCC 3 cut(s) 385, 910, 1805
BspMAI CTGCAG 2 cut(s) 1014, 1248
BspMI ACCTGC 3 cut(s) 884, 896, 1040
BspPI GGATC 4 cut(s) 315, 362, 428, 1591
BspQI GCTCTTC 1 cut(s) 488
BspT104I TTCGAA 2 cut(s) 534, 1748
BspT107I GGYRCC 3 cut(s) 383, 908, 1803
BspTNI GGTCTC 1 cut(s) 1022
BsrBI CCGCTC 2 cut(s) 1151, 1858
BsrFI RCCGGY 4 cut(s) 83, 1219, 1530, 1790
BsrI ACTGG 2 cut(s) 1517, 1696
BssAI RCCGGY 4 cut(s) 83, 1219, 1530, 1790
BssECI CCNNGG 7 cut(s) 66, 580, 808, 1090, 1132, 1485, 1869
BssMI GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
BssSI CACGAG 1 cut(s) 1682
BssT1I CCWWGG 1 cut(s) 66
Bst2BI CACGAG 1 cut(s) 1682
Bst2UI CCWGG 4 cut(s) 890, 1091, 1133, 1843
Bst4CI ACNGT 6 cut(s) 289, 472, 529, 1486, 1514, 1595
Bst6I CTCTTC 3 cut(s) 488, 553, 683
BstAPI GCANNNNNTGC 2 cut(s) 418, 1414
BstBI TTCGAA 2 cut(s) 534, 1748
BstDEI CTNAG 5 cut(s) 213, 252, 270, 1000, 1172
BstDSI CCRYGG 3 cut(s) 66, 808, 1485
BstEII GGTNACC 1 cut(s) 871
BstF5I GGATG 6 cut(s) 156, 402, 650, 1074, 1143, 1284
BstFNI CGCG 5 cut(s) 625, 827, 1343, 1433, 1754
BstH2I RGCGCY 1 cut(s) 1172
BstHHI GCGC 4 cut(s) 251, 827, 1171, 1433
BstKTI GATC 9 cut(s) 310, 336, 370, 436, 487, 882, 987, 1336, 1586
BstMAI GTCTC 3 cut(s) 644, 1022, 1679
BstMBI GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
BstMCI CGRYCG 1 cut(s) 987
BstNI CCWGG 4 cut(s) 890, 1091, 1133, 1843
BstNSI RCATGY 1 cut(s) 1009
BstPI GGTNACC 1 cut(s) 871
BstSFI CTRYAG 2 cut(s) 1010, 1244
BstUI CGCG 5 cut(s) 625, 827, 1343, 1433, 1754
BstV1I GCAGC 7 cut(s) 238, 412, 424, 433, 996, 1401, 1761
BstV2I GAAGAC 5 cut(s) 129, 183, 543, 651, 1390
BstX2I RGATCY 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 868
BstYI RGATCY 1 cut(s) 307
BsuI GTATCC 1 cut(s) 63
BtgI CCRYGG 3 cut(s) 66, 808, 1485
BtgZI GCGATG 1 cut(s) 1846
BtrI CACGTC 3 cut(s) 318, 1111, 1291
BtsCI GGATG 6 cut(s) 156, 402, 650, 1074, 1143, 1284
BtsI GCAGTG 1 cut(s) 1148
BtsIMutI CAGTG 4 cut(s) 1148, 1458, 1524, 1703
BveI ACCTGC 3 cut(s) 884, 896, 1040
CfoI GCGC 4 cut(s) 251, 827, 1171, 1433
Cfr10I RCCGGY 4 cut(s) 83, 1219, 1530, 1790
CpoI CGGWCCG 1 cut(s) 1361
CseI GACGC 3 cut(s) 626, 1332, 1349
Csp6I GTAC 4 cut(s) 37, 178, 1621, 1804
CspAI ACCGGT 1 cut(s) 1530
CspI CGGWCCG 1 cut(s) 1361
CviAII CATG 9 cut(s) 67, 199, 241, 331, 817, 913, 1006, 1525, 1900
CviQI GTAC 4 cut(s) 37, 178, 1621, 1804
DdeI CTNAG 5 cut(s) 213, 252, 270, 1000, 1172
DpnI GATC 9 cut(s) 309, 335, 369, 435, 486, 881, 986, 1335, 1585
DpnII GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
EaeI YGGCCR 1 cut(s) 1329
Eam1104I CTCTTC 3 cut(s) 488, 553, 683
EarI CTCTTC 3 cut(s) 488, 553, 683
EciI GGCGGA 4 cut(s) 70, 218, 320, 1809
Ecl136II GAGCTC 2 cut(s) 631, 673
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 3 cut(s) 633, 675, 1708
Eco31I GGTCTC 1 cut(s) 1022
Eco47I GGWCC 4 cut(s) 1361, 1514, 1528, 1666
Eco53kI GAGCTC 2 cut(s) 631, 673
Eco88I CYCGRG 1 cut(s) 447
Eco91I GGTNACC 1 cut(s) 871
EcoICRI GAGCTC 2 cut(s) 631, 673
EcoO65I GGTNACC 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 4 cut(s) 888, 1089, 1131, 1841
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 3 cut(s) 633, 675, 1708
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 2 cut(s) 644, 1679
FaeI CATG 9 cut(s) 70, 202, 244, 334, 820, 916, 1009, 1528, 1903
FaqI GGGAC 1 cut(s) 1715
FatI CATG 9 cut(s) 66, 198, 240, 330, 816, 912, 1005, 1524, 1899
FblI GTMKAC 3 cut(s) 314, 429, 969
FokI GGATG 6 cut(s) 163, 409, 637, 1061, 1150, 1271
FriOI GRGCYC 3 cut(s) 633, 675, 1708
GlaI GCGC 4 cut(s) 250, 826, 1170, 1432
GsaI CCCAGC 3 cut(s) 136, 396, 767
GsuI CTGGAG 2 cut(s) 1073, 1598
HaeII RGCGCY 1 cut(s) 1172
HgaI GACGC 3 cut(s) 626, 1332, 1349
HhaI GCGC 4 cut(s) 251, 827, 1171, 1433
Hin1II CATG 9 cut(s) 70, 202, 244, 334, 820, 916, 1009, 1528, 1903
Hin6I GCGC 4 cut(s) 249, 825, 1169, 1431
HinP1I GCGC 4 cut(s) 249, 825, 1169, 1431
HincII GTYRAC 3 cut(s) 315, 430, 1390
HindII GTYRAC 3 cut(s) 315, 430, 1390
HindIII AAGCTT 1 cut(s) 218
HphI GGTGA 9 cut(s) 457, 507, 589, 744, 883, 1051, 1468, 1618, 1898
Hpy188III TCNNGA 6 cut(s) 56, 254, 1123, 1337, 1598, 1675
HpyAV CCTTC 6 cut(s) 713, 740, 940, 1006, 1435, 1652
HpyCH4III ACNGT 6 cut(s) 289, 472, 529, 1486, 1514, 1595
HpyCH4IV ACGT 5 cut(s) 317, 1110, 1290, 1644, 1688
HpyF3I CTNAG 5 cut(s) 213, 252, 270, 1000, 1172
HpySE526I ACGT 5 cut(s) 317, 1110, 1290, 1644, 1688
Hsp92II CATG 9 cut(s) 70, 202, 244, 334, 820, 916, 1009, 1528, 1903
HspAI GCGC 4 cut(s) 249, 825, 1169, 1431
KpnI GGTACC 1 cut(s) 1807
KroI GCCGGC 3 cut(s) 83, 1219, 1790
KroNI GCCGGC 3 cut(s) 85, 1221, 1792
Kzo9I GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 5 cut(s) 10, 628, 678, 1492, 1551
Lsp1109I GCAGC 7 cut(s) 238, 412, 424, 433, 996, 1401, 1761
LweI GCATC 5 cut(s) 387, 1005, 1128, 1218, 1338
MaeII ACGT 5 cut(s) 317, 1110, 1290, 1644, 1688
MaeIII GTNAC 2 cut(s) 871, 1480
MalI GATC 9 cut(s) 309, 335, 369, 435, 486, 881, 986, 1335, 1585
MbiI CCGCTC 2 cut(s) 1151, 1858
MboI GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
MfeI CAATTG 1 cut(s) 1271
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 4 cut(s) 15, 633, 675, 1708
MluCI AATT 4 cut(s) 236, 361, 1046, 1271
MluI ACGCGT 2 cut(s) 1341, 1752
MmeI TCCRAC 4 cut(s) 823, 1238, 1260, 1593
MreI CGCCGGCG 2 cut(s) 83, 1790
MroNI GCCGGC 3 cut(s) 83, 1219, 1790
MroXI GAANNNNTTC 2 cut(s) 848, 1754
MseI TTAA 1 cut(s) 374
MspA1I CMGCKG 2 cut(s) 28, 490
MunI CAATTG 1 cut(s) 1271
MvaI CCWGG 4 cut(s) 890, 1091, 1133, 1843
MvnI CGCG 5 cut(s) 625, 827, 1343, 1433, 1754
NaeI GCCGGC 3 cut(s) 85, 1221, 1792
NciI CCSGG 9 cut(s) 302, 582, 655, 1179, 1428, 1670, 1708, 1733, 1808
NcoI CCATGG 1 cut(s) 66
NdeII GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
NgoMIV GCCGGC 3 cut(s) 83, 1219, 1790
NlaIII CATG 9 cut(s) 70, 202, 244, 334, 820, 916, 1009, 1528, 1903
NlaIV GGNNCC 3 cut(s) 385, 910, 1805
NmeAIII GCCGAG 3 cut(s) 769, 1697, 1850
NmuCI GTSAC 2 cut(s) 871, 1480
NspI RCATGY 1 cut(s) 1009
NspV TTCGAA 2 cut(s) 534, 1748
PaqCI CACCTGC 1 cut(s) 896
PciSI GCTCTTC 1 cut(s) 488
PcsI WCGNNNNNNNCGW 2 cut(s) 961, 1680
PdiI GCCGGC 3 cut(s) 85, 1221, 1792
PdmI GAANNNNTTC 2 cut(s) 848, 1754
PfeI GAWTC 7 cut(s) 31, 128, 138, 614, 779, 957, 1082
PflFI GACNNNGTC 1 cut(s) 1670
PflMI CCANNNNNTGG 1 cut(s) 117
PinAI ACCGGT 1 cut(s) 1530
Ple19I CGATCG 1 cut(s) 987
PsiI TTATAA 1 cut(s) 1890
Psp124BI GAGCTC 2 cut(s) 633, 675
Psp6I CCWGG 4 cut(s) 888, 1089, 1131, 1841
PspEI GGTNACC 1 cut(s) 871
PspFI CCCAGC 3 cut(s) 132, 392, 763
PspGI CCWGG 4 cut(s) 888, 1089, 1131, 1841
PspN4I GGNNCC 3 cut(s) 385, 910, 1805
PstI CTGCAG 2 cut(s) 1014, 1248
PsuI RGATCY 1 cut(s) 307
PsyI GACNNNGTC 1 cut(s) 1670
PvuI CGATCG 1 cut(s) 987
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 4 cut(s) 38, 179, 1622, 1805
RsaNI GTAC 4 cut(s) 37, 178, 1621, 1804
Rsr2I CGGWCCG 1 cut(s) 1361
RsrII CGGWCCG 1 cut(s) 1361
SacI GAGCTC 2 cut(s) 633, 675
SalI GTCGAC 2 cut(s) 313, 428
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 1 cut(s) 374
Sau3AI GATC 9 cut(s) 307, 333, 367, 433, 484, 879, 984, 1333, 1583
SduI GDGCHC 4 cut(s) 15, 633, 675, 1708
SfaNI GCATC 5 cut(s) 387, 1005, 1128, 1218, 1338
SfcI CTRYAG 2 cut(s) 1010, 1244
SfuI TTCGAA 2 cut(s) 534, 1748
SgrAI CRCCGGYG 2 cut(s) 83, 1790
SgrDI CGTCGACG 1 cut(s) 428
SinI GGWCC 4 cut(s) 1361, 1514, 1528, 1666
SmlI CTYRAG 3 cut(s) 221, 323, 632
SmoI CTYRAG 3 cut(s) 221, 323, 632
Sse9I AATT 4 cut(s) 236, 361, 1046, 1271
SstI GAGCTC 2 cut(s) 633, 675
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 6 cut(s) 289, 472, 529, 1486, 1514, 1595
TaiI ACGT 5 cut(s) 320, 1113, 1293, 1647, 1691
TaqII GACCGA 4 cut(s) 177, 1378, 1384, 1885
TasI AATT 4 cut(s) 236, 361, 1046, 1271
TatI WGTACW 1 cut(s) 1620
TauI GCSGC 8 cut(s) 49, 90, 93, 96, 99, 695, 1185, 1219
TfiI GAWTC 7 cut(s) 31, 128, 138, 614, 779, 957, 1082
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 4 cut(s) 1148, 1458, 1524, 1703
TseFI GTSAC 2 cut(s) 871, 1480
TseI GCWGC 7 cut(s) 226, 400, 412, 421, 1009, 1414, 1774
Tsp45I GTSAC 2 cut(s) 871, 1480
TspDTI ATGAA 6 cut(s) 17, 229, 1142, 1481, 1513, 1727
TspGWI ACGGA 4 cut(s) 426, 609, 807, 825
TspRI CASTG 4 cut(s) 1148, 1458, 1524, 1703
Tth111I GACNNNGTC 1 cut(s) 1670
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 4 cut(s) 1361, 1514, 1528, 1666
XapI RAATTY 2 cut(s) 236, 1046
XceI RCATGY 1 cut(s) 1009
XmiI GTMKAC 3 cut(s) 314, 429, 969
XmnI GAANNNNTTC 2 cut(s) 848, 1754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.