pycom16g02050

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011800
Physical Location & Seq
Forward (+)
1285164 .. 1287068
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g02050.1

Sequence Viewer

Length: 1905 bp
ATGAAAGGGGAGCACCAGACTCATCAGCTGAATCAGTACCCGCCGCCGCAGCATCCAGACCCGTCCATGGCTTCCACCTCCACCGGCGGCGGCTTCGGCAAGGCCAAGATGTGGGAAGACGAATCCCACCGAATCGACGGCGGGATGGACGAGCTTCTGGCGGTGTTGGGGTACAAGGTTCGGTCTTCGGACATGGCGGAGGTTGCTCAGAAGCTTGAGCAGCTTGAAGAATTCATGGGTTGTGCTCAGGAAGATGGACTCTCTCAGCTCGCTTCTGACACCGTTCAATATAACCCGGCGGATCTGTCGACGTGGCTTGAGAGCATGATCTCCGAGATAAACCTCCCGCCTCCTAATTTCGATCCTTTAATGGGCGGTGCCGTCGCTGGGATGCAGCCTAATCAGCAGCAAGTGCAGCTCGTTGACGACCCGTTTTTAGCTAGGGGGGAGTCGTCCATCACCACCGTCGATTTCCCAGATCAGCGGAAGAGCAAATCCATTAGCACATCACCTCCCCAAACTGTGTTCGAAGACTGCAACTCTTCTTCTAGCTACTACGACTTCAAATCTATCCCCGGAAACGCCGTGTTCACCCAAACCCGCCTCGACTCTCCGTCGCGGGAGCCCAAGCGTCTCAAATCCTCATCCGGGTCTTCTCCCTCCGAACTCCTCTTCAACCGCCCCGCCGCCTCTTCCCTTCCACAACCACAACAACCCATCTCCCTCCCCGCCACCGCCGAGTCATCACCAACCCGCCCAGCCCTCATTGTCGATTCGCAGGAAAACGGAGTCCGACTCGTTCACGGCCTCATGGCCTGCGCGGAAGCCGTCCAGCAGAACAACTTCAACCTCGCCAAAGCTCTAGTGACCCAGATCGGCTACCTGGCAGGTTCACAAGCAGGTGCCATGCGTAAAGTCGCCACCTTCTTCGCCGAAGCTCTGGCCCAACGAATCTTCCAAGTCTACCCTCAGTCGCCGATCGACCACTCCTTCTCAGACATGCTGCAGATGCACTTCTACGAGACCTGCCCCAACCTGAAATTCGCCCACTTCACCGCCAATCAAGCCATCCTCGAATCCCTCCAGGGAAAAACCAGAGTCCACGTTATCGACTTCTCGATGAACCAGGGGATGCAGTGGCCCGCTCTGATGCAGGCGTTGGCGCTCAGACCCGGCGGCCCGCCCGCTTTTCGGCTCACGGGTATCGGGCCGCCAGCTTCGGATAACTCCGACCATCTGCAGGAGGTGGGTTGGAAGCTCGCCCAATTGGCTGAAACCATCCACGTCGAGTTTGAATACAGAGGATTTGTGGCAAACAGCTTGGCCGATCTTGACGCGTCGATGCTTGAACTCAGACCGAGTGAGGTTGAGTCGGTGGCGGTCAACTCGGTCTTCGAGTTGCACAAGCTGCTGGCCCGACCGGGTGCGATTGAGAAGGTGTTATCGGTGGTGAAGCAAATGAAGCCGGAGATTGTGACGGTGGTGGAGCAGGAGGCGAACCACAACGGTCCGGTGTTCATGGACCGGTTCAACGAGTCGCTCCACTATTACTCGACCCTGTTTGACTCGCTGGAGGGATCGGCGAACAGTCGGGATAAGGTGATGTCGGAAGTGTACTTGGGGAAGCAGATCTGCAACGTGGTGGCGTGCGAAGGGGTGGACCGGGTCGAGAGGCACGAGACGTTGACCCAGTGGCGAGCCCGGTTCGGCTCGGCAGATTTCGTCCCGGTTCATCTCGGTTCGAACGCGTTCAAGCAAGCGAGTATGCTGCTGGCGTTGTTCGCCGGCGGAGATGGGTACCGGGTGGAGGAGAACGACGGGTGTATGATGTTGGCCTGGCACACTCGCCCGCTTATCGCCACCTCGGCTTGGAAACCCGCTTATAACTCGGTCATGGCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000910 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006808 GO:0006810 GO:0006950 GO:0006970 GO:0006972 GO:0007049 GO:0007154 GO:0007165 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009739 GO:0009740 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009889 GO:0009892 GO:0009893 GO:0009894 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010187 GO:0010218 GO:0010232 GO:0010233 GO:0010453 GO:0010454 GO:0010468 GO:0010476 GO:0010556 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010648 GO:0014070 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031326 GO:0032501 GO:0032870 GO:0033206 GO:0033993 GO:0035690 GO:0040008 GO:0042176 GO:0042221 GO:0042493 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0045926 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048638 GO:0048640 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051246 GO:0051252 GO:0051301 GO:0051321 GO:0051716 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071370 GO:0071395 GO:0071396 GO:0071407 GO:0071446 GO:0071495 GO:0080050 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1900032 GO:1900033 GO:1900140 GO:1901363 GO:1901700 GO:1901701 GO:1903046 GO:1903506 GO:1905613 GO:1905614 GO:1905622 GO:2000024 GO:2000026 GO:2000033 GO:2000034 GO:2000112 GO:2000241 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
DELLA GRAS

Protein Analysis

635

Amino Acids

69.66

Weight (kDa)

5.23

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DELLA PF12041 50 - 117 6.4e-34 Transcriptional regulator DELLA protein N terminal
GRAS PF03514 266 - 625 3.4e-132 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011800)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14920 AT2G01570
fragaria_vesca FvH4_4g34110
malus_domestica MD13G1022100.v1.1 MD16G1023300.v1.1
prunus_persica Prupe.1G329600_v2.0.a1
pyrus_communis pycom13g02070 pycom16g02040 pycom16g02050
rosa_chinensis RchiOBHm_Chr4g0443621
rosa_laevigata RLG00000005909
rosa_multiflora Rmu_sc0001118.1_g000014
rosa_roxburghii Rroxscaffold_5G00384170
rosa_samantha Rh4AG403600 Rh4AG405600 Rh4BG416600 Rh4CG431100 Rh4DG412200
rosa_wichuraiana Rw4G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1884
AarI CACCTGC 1 cut(s) 890
Acc36I ACCTGC 3 cut(s) 878, 890, 1034
Acc65I GGTACC 1 cut(s) 1797
AccB1I GGYRCC 3 cut(s) 377, 902, 1797
AccB7I CCANNNNNTGG 1 cut(s) 111
AccBSI CCGCTC 1 cut(s) 1145
AccI GTMKAC 2 cut(s) 308, 963
AccII CGCG 4 cut(s) 619, 821, 1337, 1748
AclWI GGATC 3 cut(s) 309, 356, 1585
AcoI YGGCCR 1 cut(s) 1323
AcsI RAATTY 2 cut(s) 230, 1040
AfaI GTAC 4 cut(s) 38, 173, 1616, 1799
AfiI CCNNNNNNNGG 9 cut(s) 67, 111, 371, 387, 648, 1191, 1240, 1807, 1869
AflIII ACRYGT 2 cut(s) 1335, 1746
AgeI ACCGGT 1 cut(s) 1524
AgsI TTSAA 9 cut(s) 227, 287, 565, 676, 847, 1295, 1349, 1531, 1753
AhdI GACNNNNNGTC 1 cut(s) 613
AjiI CACGTC 2 cut(s) 312, 1285
AjnI CCWGG 4 cut(s) 882, 1083, 1125, 1835
AjuI GAANNNNNNNTTGG 2 cut(s) 1025, 1057
Alw21I GWGCWC 2 cut(s) 15, 247
Alw26I GTCTC 3 cut(s) 638, 1016, 1673
AlwI GGATC 3 cut(s) 309, 356, 1585
ApeKI GCWGC 8 cut(s) 49, 220, 394, 406, 415, 1003, 1408, 1768
ApoI RAATTY 2 cut(s) 230, 1040
AsiGI ACCGGT 1 cut(s) 1524
Asp700I GAANNNNTTC 2 cut(s) 842, 1748
Asp718I GGTACC 1 cut(s) 1797
AspLEI GCGC 2 cut(s) 821, 1165
AspS9I GGNCC 8 cut(s) 943, 1140, 1178, 1208, 1414, 1508, 1522, 1660
AsuC2I CCSGG 9 cut(s) 296, 576, 649, 1173, 1422, 1664, 1702, 1727, 1802
AsuHPI GGTGA 7 cut(s) 451, 501, 583, 738, 1045, 1462, 1612
AsuII TTCGAA 2 cut(s) 528, 1742
AvaII GGWCC 3 cut(s) 1508, 1522, 1660
BanI GGYRCC 3 cut(s) 377, 902, 1797
BanII GRGCYC 2 cut(s) 627, 1702
BauI CACGAG 1 cut(s) 1676
BbsI GAAGAC 5 cut(s) 123, 177, 537, 645, 1384
Bbv12I GWGCWC 2 cut(s) 15, 247
BbvI GCAGC 8 cut(s) 61, 232, 406, 418, 427, 990, 1395, 1755
BccI CCATC 8 cut(s) 139, 248, 464, 725, 1076, 1242, 1286, 1787
BceAI ACGGC 5 cut(s) 154, 365, 569, 812, 820
BcgI CGANNNNNNTGC 2 cut(s) 1750, 1784
BciT130I CCWGG 4 cut(s) 884, 1085, 1127, 1837
BcnI CCSGG 9 cut(s) 296, 576, 649, 1173, 1422, 1664, 1702, 1727, 1802
BcoDI GTCTC 3 cut(s) 638, 1016, 1673
BfaI CTAG 3 cut(s) 441, 549, 863
BfmI CTRYAG 2 cut(s) 1004, 1238
BfoI RGCGCY 1 cut(s) 1166
BfuAI ACCTGC 3 cut(s) 878, 890, 1034
BglI GCCNNNNNGGC 2 cut(s) 1268, 1865
BglII AGATCT 1 cut(s) 1629
Bme18I GGWCC 3 cut(s) 1508, 1522, 1660
BmeRI GACNNNNNGTC 1 cut(s) 613
BmgBI CACGTC 2 cut(s) 312, 1285
BmgT120I GGNCC 8 cut(s) 943, 1140, 1178, 1208, 1414, 1508, 1522, 1660
BmiI GGNNCC 4 cut(s) 379, 624, 904, 1799
BmrI ACTGGG 1 cut(s) 1684
BmsI GCATC 6 cut(s) 61, 381, 999, 1122, 1140, 1332
BmuI ACTGGG 1 cut(s) 1684
BpiI GAAGAC 5 cut(s) 123, 177, 537, 645, 1384
BplI GAGNNNNNCTC 2 cut(s) 780, 812
BpmI CTGGAG 2 cut(s) 1067, 1592
Bpu10I CCTNAGC 1 cut(s) 246
Bpu14I TTCGAA 2 cut(s) 528, 1742
BpuEI CTTGAG 2 cut(s) 236, 338
BpuMI CCSGG 9 cut(s) 296, 576, 649, 1173, 1422, 1664, 1702, 1727, 1802
BsaI GGTCTC 1 cut(s) 1016
BsaJI CCNNGG 5 cut(s) 66, 574, 1084, 1126, 1863
BsaWI WCCGGW 2 cut(s) 1510, 1524
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bsc4I CCNNNNNNNGG 9 cut(s) 67, 111, 371, 387, 648, 1191, 1240, 1807, 1869
Bse118I RCCGGY 3 cut(s) 83, 1524, 1784
Bse1I ACTGG 1 cut(s) 1690
BseBI CCWGG 4 cut(s) 884, 1085, 1127, 1837
BseDI CCNNGG 5 cut(s) 66, 574, 1084, 1126, 1863
BseGI GGATG 7 cut(s) 52, 150, 396, 644, 1068, 1137, 1278
BseLI CCNNNNNNNGG 9 cut(s) 67, 111, 371, 387, 648, 1191, 1240, 1807, 1869
BseMII CTCAG 7 cut(s) 221, 260, 278, 983, 1008, 1180, 1366
BseNI ACTGG 1 cut(s) 1690
BseRI GAGGAG 2 cut(s) 659, 1823
BseXI GCAGC 8 cut(s) 61, 232, 406, 418, 427, 990, 1395, 1755
BseYI CCCAGC 2 cut(s) 386, 757
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 4 cut(s) 619, 821, 1337, 1748
Bsh1285I CGRYCG 2 cut(s) 981, 1421
BshNI GGYRCC 3 cut(s) 377, 902, 1797
BshTI ACCGGT 1 cut(s) 1524
BsiEI CGRYCG 2 cut(s) 981, 1421
BsiHKAI GWGCWC 2 cut(s) 15, 247
BslFI GGGAC 1 cut(s) 1709
BslI CCNNNNNNNGG 9 cut(s) 67, 111, 371, 387, 648, 1191, 1240, 1807, 1869
BsmAI GTCTC 3 cut(s) 638, 1016, 1673
BsmBI CGTCTC 2 cut(s) 638, 1673
BsmFI GGGAC 1 cut(s) 1709
Bso31I GGTCTC 1 cut(s) 1016
Bsp119I TTCGAA 2 cut(s) 528, 1742
Bsp1286I GDGCHC 4 cut(s) 15, 247, 627, 1702
Bsp143I GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
Bsp19I CCATGG 1 cut(s) 66
BspCNI CTCAG 7 cut(s) 220, 259, 277, 982, 1007, 1179, 1365
BspFNI CGCG 4 cut(s) 619, 821, 1337, 1748
BspLI GGNNCC 4 cut(s) 379, 624, 904, 1799
BspMAI CTGCAG 2 cut(s) 1008, 1242
BspMI ACCTGC 3 cut(s) 878, 890, 1034
BspPI GGATC 3 cut(s) 309, 356, 1585
BspQI GCTCTTC 1 cut(s) 482
BspT104I TTCGAA 2 cut(s) 528, 1742
BspT107I GGYRCC 3 cut(s) 377, 902, 1797
BspTNI GGTCTC 1 cut(s) 1016
BsrBI CCGCTC 1 cut(s) 1145
BsrFI RCCGGY 3 cut(s) 83, 1524, 1784
BsrI ACTGG 1 cut(s) 1690
BssAI RCCGGY 3 cut(s) 83, 1524, 1784
BssECI CCNNGG 5 cut(s) 66, 574, 1084, 1126, 1863
BssMI GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
BssSI CACGAG 1 cut(s) 1676
BssT1I CCWWGG 1 cut(s) 66
Bst2BI CACGAG 1 cut(s) 1676
Bst2UI CCWGG 4 cut(s) 884, 1085, 1127, 1837
Bst4CI ACNGT 6 cut(s) 283, 466, 523, 1480, 1508, 1589
Bst6I CTCTTC 4 cut(s) 482, 547, 677, 697
BstAPI GCANNNNNTGC 2 cut(s) 412, 1408
BstBI TTCGAA 2 cut(s) 528, 1742
BstDEI CTNAG 7 cut(s) 207, 246, 264, 969, 994, 1166, 1352
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 7 cut(s) 52, 150, 396, 644, 1068, 1137, 1278
BstFNI CGCG 4 cut(s) 619, 821, 1337, 1748
BstH2I RGCGCY 1 cut(s) 1166
BstHHI GCGC 2 cut(s) 821, 1165
BstKTI GATC 9 cut(s) 304, 330, 364, 481, 876, 981, 1330, 1580, 1632
BstMAI GTCTC 3 cut(s) 638, 1016, 1673
BstMBI GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
BstMCI CGRYCG 2 cut(s) 981, 1421
BstNI CCWGG 4 cut(s) 884, 1085, 1127, 1837
BstNSI RCATGY 1 cut(s) 1003
BstSFI CTRYAG 2 cut(s) 1004, 1238
BstUI CGCG 4 cut(s) 619, 821, 1337, 1748
BstV1I GCAGC 8 cut(s) 61, 232, 406, 418, 427, 990, 1395, 1755
BstV2I GAAGAC 5 cut(s) 123, 177, 537, 645, 1384
BstX2I RGATCY 2 cut(s) 301, 1629
BstYI RGATCY 2 cut(s) 301, 1629
BtgI CCRYGG 1 cut(s) 66
BtrI CACGTC 2 cut(s) 312, 1285
BtsCI GGATG 7 cut(s) 52, 150, 396, 644, 1068, 1137, 1278
BtsI GCAGTG 1 cut(s) 1142
BtsIMutI CAGTG 3 cut(s) 1142, 1697, 1900
BveI ACCTGC 3 cut(s) 878, 890, 1034
CfoI GCGC 2 cut(s) 821, 1165
Cfr10I RCCGGY 3 cut(s) 83, 1524, 1784
Cfr13I GGNCC 8 cut(s) 943, 1140, 1178, 1208, 1414, 1508, 1522, 1660
CpoI CGGWCCG 1 cut(s) 1508
CseI GACGC 3 cut(s) 620, 1326, 1343
Csp6I GTAC 4 cut(s) 37, 172, 1615, 1798
CspAI ACCGGT 1 cut(s) 1524
CspI CGGWCCG 1 cut(s) 1508
CviAII CATG 9 cut(s) 67, 193, 235, 325, 811, 907, 1000, 1519, 1894
CviQI GTAC 4 cut(s) 37, 172, 1615, 1798
DdeI CTNAG 7 cut(s) 207, 246, 264, 969, 994, 1166, 1352
DpnI GATC 9 cut(s) 303, 329, 363, 480, 875, 980, 1329, 1579, 1631
DpnII GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
DriI GACNNNNNGTC 1 cut(s) 613
EaeI YGGCCR 1 cut(s) 1323
Eam1104I CTCTTC 4 cut(s) 482, 547, 677, 697
Eam1105I GACNNNNNGTC 1 cut(s) 613
EarI CTCTTC 4 cut(s) 482, 547, 677, 697
EciI GGCGGA 3 cut(s) 212, 314, 1803
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 627, 1702
Eco31I GGTCTC 1 cut(s) 1016
Eco47I GGWCC 3 cut(s) 1508, 1522, 1660
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 4 cut(s) 882, 1083, 1125, 1835
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 2 cut(s) 627, 1702
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 2 cut(s) 638, 1673
FaeI CATG 9 cut(s) 70, 196, 238, 328, 814, 910, 1003, 1522, 1897
FaqI GGGAC 1 cut(s) 1709
FatI CATG 9 cut(s) 66, 192, 234, 324, 810, 906, 999, 1518, 1893
FblI GTMKAC 2 cut(s) 308, 963
FokI GGATG 7 cut(s) 39, 157, 403, 631, 1055, 1144, 1265
FriOI GRGCYC 2 cut(s) 627, 1702
FspBI CTAG 3 cut(s) 441, 549, 863
GlaI GCGC 2 cut(s) 820, 1164
GsaI CCCAGC 2 cut(s) 390, 761
GsuI CTGGAG 2 cut(s) 1067, 1592
HaeII RGCGCY 1 cut(s) 1166
HgaI GACGC 3 cut(s) 620, 1326, 1343
HhaI GCGC 2 cut(s) 821, 1165
Hin1II CATG 9 cut(s) 70, 196, 238, 328, 814, 910, 1003, 1522, 1897
Hin6I GCGC 2 cut(s) 819, 1163
HinP1I GCGC 2 cut(s) 819, 1163
HincII GTYRAC 4 cut(s) 309, 424, 1384, 1686
HindII GTYRAC 4 cut(s) 309, 424, 1384, 1686
HindIII AAGCTT 1 cut(s) 212
HphI GGTGA 7 cut(s) 451, 501, 583, 738, 1045, 1462, 1612
Hpy188III TCNNGA 6 cut(s) 56, 248, 1117, 1331, 1592, 1669
Hpy99I CGWCG 8 cut(s) 140, 313, 386, 470, 619, 1289, 1342, 1820
HpyAV CCTTC 5 cut(s) 707, 934, 1000, 1429, 1646
HpyCH4III ACNGT 6 cut(s) 283, 466, 523, 1480, 1508, 1589
HpyCH4IV ACGT 5 cut(s) 311, 1104, 1284, 1638, 1682
HpyF3I CTNAG 7 cut(s) 207, 246, 264, 969, 994, 1166, 1352
HpySE526I ACGT 5 cut(s) 311, 1104, 1284, 1638, 1682
Hsp92II CATG 9 cut(s) 70, 196, 238, 328, 814, 910, 1003, 1522, 1897
HspAI GCGC 2 cut(s) 819, 1163
KpnI GGTACC 1 cut(s) 1801
KroI GCCGGC 1 cut(s) 1784
KroNI GCCGGC 1 cut(s) 1786
Kzo9I GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
LguI GCTCTTC 1 cut(s) 482
LmnI GCTCC 4 cut(s) 10, 622, 1486, 1545
Lsp1109I GCAGC 8 cut(s) 61, 232, 406, 418, 427, 990, 1395, 1755
LweI GCATC 6 cut(s) 61, 381, 999, 1122, 1140, 1332
MaeI CTAG 3 cut(s) 441, 549, 863
MaeII ACGT 5 cut(s) 311, 1104, 1284, 1638, 1682
MaeIII GTNAC 2 cut(s) 865, 1474
MalI GATC 9 cut(s) 303, 329, 363, 480, 875, 980, 1329, 1579, 1631
MbiI CCGCTC 1 cut(s) 1145
MboI GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
MfeI CAATTG 1 cut(s) 1265
MflI RGATCY 2 cut(s) 301, 1629
MhlI GDGCHC 4 cut(s) 15, 247, 627, 1702
MluCI AATT 4 cut(s) 230, 355, 1040, 1265
MluI ACGCGT 2 cut(s) 1335, 1746
MmeI TCCRAC 4 cut(s) 817, 1232, 1254, 1587
MreI CGCCGGCG 1 cut(s) 1784
MroNI GCCGGC 1 cut(s) 1784
MroXI GAANNNNTTC 2 cut(s) 842, 1748
MseI TTAA 1 cut(s) 368
MspA1I CMGCKG 2 cut(s) 28, 484
MunI CAATTG 1 cut(s) 1265
MvaI CCWGG 4 cut(s) 884, 1085, 1127, 1837
MvnI CGCG 4 cut(s) 619, 821, 1337, 1748
NaeI GCCGGC 1 cut(s) 1786
NciI CCSGG 9 cut(s) 296, 576, 649, 1173, 1422, 1664, 1702, 1727, 1802
NcoI CCATGG 1 cut(s) 66
NdeII GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
NgoMIV GCCGGC 1 cut(s) 1784
NlaIII CATG 9 cut(s) 70, 196, 238, 328, 814, 910, 1003, 1522, 1897
NlaIV GGNNCC 4 cut(s) 379, 624, 904, 1799
NmeAIII GCCGAG 3 cut(s) 763, 1691, 1844
NmuCI GTSAC 2 cut(s) 865, 1474
NspI RCATGY 1 cut(s) 1003
NspV TTCGAA 2 cut(s) 528, 1742
PaqCI CACCTGC 1 cut(s) 890
PciSI GCTCTTC 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 1674
PdiI GCCGGC 1 cut(s) 1786
PdmI GAANNNNTTC 2 cut(s) 842, 1748
PfeI GAWTC 6 cut(s) 31, 122, 132, 773, 951, 1076
PflFI GACNNNGTC 1 cut(s) 1664
PflMI CCANNNNNTGG 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 1524
Ple19I CGATCG 1 cut(s) 981
PsiI TTATAA 1 cut(s) 1884
Psp6I CCWGG 4 cut(s) 882, 1083, 1125, 1835
PspFI CCCAGC 2 cut(s) 386, 757
PspGI CCWGG 4 cut(s) 882, 1083, 1125, 1835
PspN4I GGNNCC 4 cut(s) 379, 624, 904, 1799
PspPI GGNCC 8 cut(s) 943, 1140, 1178, 1208, 1414, 1508, 1522, 1660
PstI CTGCAG 2 cut(s) 1008, 1242
PsuI RGATCY 2 cut(s) 301, 1629
PsyI GACNNNGTC 1 cut(s) 1664
PvuI CGATCG 1 cut(s) 981
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 4 cut(s) 38, 173, 1616, 1799
RsaNI GTAC 4 cut(s) 37, 172, 1615, 1798
Rsr2I CGGWCCG 1 cut(s) 1508
RsrII CGGWCCG 1 cut(s) 1508
SalI GTCGAC 1 cut(s) 307
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 1 cut(s) 368
Sau3AI GATC 9 cut(s) 301, 327, 361, 478, 873, 978, 1327, 1577, 1629
Sau96I GGNCC 8 cut(s) 943, 1140, 1178, 1208, 1414, 1508, 1522, 1660
SduI GDGCHC 4 cut(s) 15, 247, 627, 1702
SfaNI GCATC 6 cut(s) 61, 381, 999, 1122, 1140, 1332
SfcI CTRYAG 2 cut(s) 1004, 1238
SfuI TTCGAA 2 cut(s) 528, 1742
SgrAI CRCCGGYG 2 cut(s) 83, 1784
SinI GGWCC 3 cut(s) 1508, 1522, 1660
SmlI CTYRAG 2 cut(s) 215, 317
SmoI CTYRAG 2 cut(s) 215, 317
Sse9I AATT 4 cut(s) 230, 355, 1040, 1265
SspMI CTAG 3 cut(s) 441, 549, 863
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 6 cut(s) 283, 466, 523, 1480, 1508, 1589
TaiI ACGT 5 cut(s) 314, 1107, 1287, 1641, 1685
TaqII GACCGA 4 cut(s) 171, 1372, 1378, 1879
TasI AATT 4 cut(s) 230, 355, 1040, 1265
TatI WGTACW 1 cut(s) 1614
TauI GCSGC 7 cut(s) 46, 49, 90, 93, 689, 1179, 1213
TfiI GAWTC 6 cut(s) 31, 122, 132, 773, 951, 1076
Tru1I TTAA 1 cut(s) 368
Tru9I TTAA 1 cut(s) 368
TscAI CASTG 2 cut(s) 1142, 1697
TseFI GTSAC 2 cut(s) 865, 1474
TseI GCWGC 8 cut(s) 49, 220, 394, 406, 415, 1003, 1408, 1768
Tsp45I GTSAC 2 cut(s) 865, 1474
TspDTI ATGAA 6 cut(s) 17, 223, 1136, 1475, 1507, 1721
TspGWI ACGGA 2 cut(s) 603, 801
TspRI CASTG 2 cut(s) 1142, 1697
Tth111I GACNNNGTC 1 cut(s) 1664
Van91I CCANNNNNTGG 1 cut(s) 111
VpaK11BI GGWCC 3 cut(s) 1508, 1522, 1660
XapI RAATTY 2 cut(s) 230, 1040
XceI RCATGY 1 cut(s) 1003
XmiI GTMKAC 2 cut(s) 308, 963
XmnI GAANNNNTTC 2 cut(s) 842, 1748
XspI CTAG 3 cut(s) 441, 549, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.