Rw4G034820

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011800
Physical Location & Seq
Forward (+)
61769582 .. 61771342
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034820.1

Sequence Viewer

Length: 1569 bp
ATGAAAAGAGAACATCACAGCCACCCCCACCACCCTAATCCAAACCCTTCCATGGCTTCCACATCCAATGATAAGGCAGGTAAGGCGGCGATGTGGGAGGACTCGCAGCGCGACGACGGCATGGATGAGCTTCTGGCTGTGCTGGGCTACAAGGTGAAGTCTTCCAACATGGCCGATGTGGCCCAGAAGCTCGAACAGCTTGAAGAGTTCATGGGGACAGTTCAGGAAGACGGGCTCACCCAGCTGGCTTCGGATACGGTTCATTACAATCCCTCGGATCTGTCGACTTGGCTGGAGAGCATGATTTCGGAGATAACCCGCCTCCGAATTTCGACCCGTAATGGCGGCGCCACCGCCGCTGAATCACCTCGCTGGAAGCTCTGTTGTGCTGGACGATTCGTTTTTGGCTCCGGCCGAGTCGTCCACCATACCTCTATCGATTTTGGCGATCAACAACACATGAACACGAACAGCAAATCTCTCAGCCCGCGAACCCAGTTCGAGGACTGCTCGTCTTCCAATTACGAGCTCAAGGCCATACCTGGAAAAGCCATTTTCAGCCAGACCCAGTTCGATTCTTCCCTAAGGGAGCCAAAACGGCTCAAACCTTGTTCTTCTTCAGATTTCTACTCCACCACCACCAACTCGTCTCTCCAACCCGTCGTCTCCCTCCCCAACAACGAGTCAACTCGCCCGGTGGTCGTGGTCGACTCGCAGGAGAACGGCGTCAGACTCGTCCACGGCCTCATGGCCTGCGCCGAAGCCGTCCAGCGCAAGAATTTCGACCTCGCTAAGGCCCTGGTGACCCAGATCAGCTACTTGGCGATTTCACAAGCCGGCGCTATGCGAAAAGTCGCCACCTTCTTCGCTGAGGCTCTAGCCCAGCGGATCTGGGGGAAGCGAGTCCATGTCATCGATTTCTCCATGAATCAGGGGATGCAATGGCCGGCTCTCATGCAGGCCCTCGCTCTCCGACCCGGCGGCCCGCCCGCGTTTCGCTTAACCGGAATCGGCCCGCCCGCCGCGGATAACTCGGACCATCTTCAGGAAGTGGGCTGGAAGCTGGCCCAGCTAGCCGAAACCATTCATGTGGAATTTGAGTACAGAGGCTTTGTTGCTAACAGCTTGGCTGATCTAGATGCTTCCATGCTGGAGCTCCGGCCGAGCGAGCTTCACAAGCTGTTGGCTCGGCCCGGTGCGATCGACAAAGTGTTGTCCGTGGTGAAGCAAATGAAGCCGGAGATTGTGACGGTTGTGGAGCAGGAAGCGAACCACAACGGTCCGGTTTTCCTGGACCGGTTCAACGAGTCGCTGCACTACTACTCGACCCTCCAGGACAAGATGATGTCGGAGGTGTACTTGGGGAAGCAAATCTTCAATGTGGTGGCATGCGAGGGACCGGACCGGGTCGAGCGGCACGAGACGCTGTCGCAGTGGCGGACCCGGTTCGACGCGTCCGGCTTCGCTCCGGTTCATCTCGGTTCCAACGCGTTCAAGCAAGCCAGCATGCTCTTGGCGCTCTTCGCGGCGGAGATGGGTACAGAGTGGAGGAGAACGACGGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000910 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006808 GO:0006810 GO:0006950 GO:0006970 GO:0006972 GO:0007049 GO:0007154 GO:0007165 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009739 GO:0009740 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009889 GO:0009892 GO:0009893 GO:0009894 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010187 GO:0010218 GO:0010232 GO:0010233 GO:0010453 GO:0010454 GO:0010468 GO:0010476 GO:0010556 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010648 GO:0014070 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031326 GO:0032501 GO:0032870 GO:0033206 GO:0033993 GO:0035690 GO:0040008 GO:0042176 GO:0042221 GO:0042493 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045595 GO:0045596 GO:0045926 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048638 GO:0048640 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051246 GO:0051252 GO:0051301 GO:0051321 GO:0051716 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071370 GO:0071395 GO:0071396 GO:0071407 GO:0071446 GO:0071495 GO:0080050 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1900032 GO:1900033 GO:1900140 GO:1901363 GO:1901700 GO:1901701 GO:1903046 GO:1903506 GO:1905613 GO:1905614 GO:1905622 GO:2000024 GO:2000026 GO:2000033 GO:2000034 GO:2000112 GO:2000241 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
DELLA GRAS

Protein Analysis

522

Amino Acids

57.91

Weight (kDa)

6.32

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DELLA PF12041 42 - 106 9.8e-34 Transcriptional regulator DELLA protein N terminal
GRAS PF03514 245 - 299 1.6e-09 GRAS domain family
GRAS PF03514 300 - 515 3.7e-70 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011800)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14920 AT2G01570
fragaria_vesca FvH4_4g34110
malus_domestica MD13G1022100.v1.1 MD16G1023300.v1.1
prunus_persica Prupe.1G329600_v2.0.a1
pyrus_communis pycom13g02070 pycom16g02040 pycom16g02050
rosa_chinensis RchiOBHm_Chr4g0443621
rosa_laevigata RLG00000005909
rosa_multiflora Rmu_sc0001118.1_g000014
rosa_roxburghii Rroxscaffold_5G00384170
rosa_samantha Rh4AG403600 Rh4AG405600 Rh4BG416600 Rh4CG431100 Rh4DG412200
rosa_wichuraiana Rw4G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 347
AccBSI CCGCTC 1 cut(s) 1414
AccI GTMKAC 2 cut(s) 284, 708
AccII CGCG 7 cut(s) 111, 490, 992, 1025, 1454, 1490, 1526
AclWI GGATC 2 cut(s) 285, 896
AcoI YGGCCR 4 cut(s) 171, 412, 944, 1160
AcsI RAATTY 3 cut(s) 327, 778, 1094
AcuI CTGAAG 2 cut(s) 603, 1028
AcyI GRCGYC 2 cut(s) 348, 726
AfaI GTAC 3 cut(s) 1103, 1358, 1540
AfiI CCNNNNNNNGG 4 cut(s) 52, 502, 793, 1045
AflIII ACRYGT 2 cut(s) 1452, 1488
AgeI ACCGGT 1 cut(s) 1296
AgsI TTSAA 4 cut(s) 203, 1303, 1378, 1495
AhdI GACNNNNNGTC 1 cut(s) 511
AjnI CCWGG 4 cut(s) 541, 798, 1290, 1332
Alw21I GWGCWC 2 cut(s) 531, 1158
Alw26I GTCTC 3 cut(s) 654, 670, 1415
AlwI GGATC 2 cut(s) 285, 896
ApeKI GCWGC 2 cut(s) 106, 1312
ApoI RAATTY 3 cut(s) 327, 778, 1094
AsiGI ACCGGT 1 cut(s) 1296
Asp700I GAANNNNTTC 1 cut(s) 1083
AspLEI GCGC 6 cut(s) 111, 350, 758, 774, 842, 1519
AsuC2I CCSGG 5 cut(s) 695, 978, 1194, 1406, 1444
AsuHPI GGTGA 5 cut(s) 166, 229, 357, 814, 1234
AsuNHI GCTAGC 1 cut(s) 1072
AvaII GGWCC 6 cut(s) 1036, 1280, 1294, 1397, 1402, 1440
AxyI CCTNAGG 1 cut(s) 584
BanI GGYRCC 1 cut(s) 347
BanII GRGCYC 3 cut(s) 237, 531, 1158
BauI CACGAG 1 cut(s) 1418
BbsI GAAGAC 3 cut(s) 153, 234, 507
Bbv12I GWGCWC 2 cut(s) 531, 1158
BbvCI CCTCAGC 1 cut(s) 870
BbvI GCAGC 2 cut(s) 118, 1299
BccI CCATC 2 cut(s) 1047, 1528
BceAI ACGGC 5 cut(s) 133, 614, 739, 749, 757
BcgI CGANNNNNNTGC 2 cut(s) 763, 797
BciT130I CCWGG 4 cut(s) 543, 800, 1292, 1334
BciVI GTATCC 1 cut(s) 247
BcnI CCSGG 5 cut(s) 695, 978, 1194, 1406, 1444
BcoDI GTCTC 3 cut(s) 654, 670, 1415
BfaI CTAG 3 cut(s) 878, 1073, 1136
BfoI RGCGCY 3 cut(s) 351, 843, 1520
BfuAI ACCTGC 1 cut(s) 68
BfuI GTATCC 1 cut(s) 247
BglI GCCNNNNNGGC 2 cut(s) 179, 598
BisI GCNGC 9 cut(s) 87, 107, 346, 357, 982, 1023, 1313, 1415, 1527
BlsI GCNGC 9 cut(s) 88, 108, 347, 358, 983, 1024, 1314, 1416, 1528
Bme1390I CCNGG 9 cut(s) 543, 695, 800, 978, 1194, 1292, 1334, 1406, 1444
Bme18I GGWCC 6 cut(s) 1036, 1280, 1294, 1397, 1402, 1440
BmeRI GACNNNNNGTC 1 cut(s) 511
BmiI GGNNCC 6 cut(s) 349, 409, 591, 1398, 1442, 1483
BmrFI CCNGG 9 cut(s) 543, 695, 800, 978, 1194, 1292, 1334, 1406, 1444
BmrI ACTGGG 2 cut(s) 490, 562
BmsI GCATC 2 cut(s) 927, 1129
BmtI GCTAGC 1 cut(s) 1076
BmuI ACTGGG 2 cut(s) 490, 562
BpiI GAAGAC 3 cut(s) 153, 234, 507
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 3 cut(s) 314, 1172, 1316
Bpu10I CCTNAGC 2 cut(s) 792, 870
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 5 cut(s) 695, 978, 1194, 1406, 1444
Bsa29I ATCGAT 2 cut(s) 438, 915
BsaHI GRCGYC 2 cut(s) 348, 726
BsaJI CCNNGG 6 cut(s) 51, 273, 739, 798, 1023, 1218
BsaWI WCCGGW 5 cut(s) 1004, 1282, 1296, 1399, 1468
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 502, 793, 1045
Bse118I RCCGGY 3 cut(s) 836, 946, 1296
Bse1I ACTGG 2 cut(s) 496, 568
Bse21I CCTNAGG 1 cut(s) 584
Bse3DI GCAATG 1 cut(s) 947
BseBI CCWGG 4 cut(s) 543, 800, 1292, 1334
BseCI ATCGAT 2 cut(s) 438, 915
BseDI CCNNGG 6 cut(s) 51, 273, 739, 798, 1023, 1218
BseGI GGATG 3 cut(s) 62, 130, 942
BseLI CCNNNNNNNGG 4 cut(s) 52, 502, 793, 1045
BseMI GCAATG 1 cut(s) 947
BseMII CTCAG 2 cut(s) 496, 861
BseNI ACTGG 2 cut(s) 496, 568
BseRI GAGGAG 1 cut(s) 1564
BseX3I CGGCCG 2 cut(s) 412, 1160
BseXI GCAGC 2 cut(s) 118, 1299
BseYI CCCAGC 4 cut(s) 142, 240, 882, 1068
BsgI GTGCAG 1 cut(s) 1298
Bsh1236I CGCG 7 cut(s) 111, 490, 992, 1025, 1454, 1490, 1526
Bsh1285I CGRYCG 3 cut(s) 415, 1163, 1203
BshNI GGYRCC 1 cut(s) 347
BshTI ACCGGT 1 cut(s) 1296
BshVI ATCGAT 2 cut(s) 438, 915
BsiEI CGRYCG 3 cut(s) 415, 1163, 1203
BsiHKAI GWGCWC 2 cut(s) 531, 1158
BslFI GGGAC 2 cut(s) 229, 1410
BslI CCNNNNNNNGG 4 cut(s) 52, 502, 793, 1045
BsmAI GTCTC 3 cut(s) 654, 670, 1415
BsmBI CGTCTC 3 cut(s) 654, 670, 1415
BsmFI GGGAC 2 cut(s) 229, 1410
Bsp1286I GDGCHC 3 cut(s) 237, 531, 1158
Bsp143I GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
Bsp19I CCATGG 1 cut(s) 51
BspCNI CTCAG 2 cut(s) 495, 862
BspDI ATCGAT 2 cut(s) 438, 915
BspFNI CGCG 7 cut(s) 111, 490, 992, 1025, 1454, 1490, 1526
BspLI GGNNCC 6 cut(s) 349, 409, 591, 1398, 1442, 1483
BspMI ACCTGC 1 cut(s) 68
BspOI GCTAGC 1 cut(s) 1076
BspPI GGATC 2 cut(s) 285, 896
BspQI GCTCTTC 1 cut(s) 1526
BspT107I GGYRCC 1 cut(s) 347
BsrBI CCGCTC 1 cut(s) 1414
BsrDI GCAATG 1 cut(s) 947
BsrFI RCCGGY 3 cut(s) 836, 946, 1296
BsrI ACTGG 2 cut(s) 496, 568
BssAI RCCGGY 3 cut(s) 836, 946, 1296
BssECI CCNNGG 6 cut(s) 51, 273, 739, 798, 1023, 1218
BssMI GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
BssNI GRCGYC 2 cut(s) 348, 726
BssSI CACGAG 1 cut(s) 1418
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 1418
Bst2UI CCWGG 4 cut(s) 543, 800, 1292, 1334
Bst4CI ACNGT 4 cut(s) 220, 259, 1252, 1280
Bst6I CTCTTC 2 cut(s) 198, 1526
BstACI GRCGYC 2 cut(s) 348, 726
BstDEI CTNAG 4 cut(s) 482, 584, 792, 870
BstDSI CCRYGG 4 cut(s) 51, 739, 1023, 1218
BstEII GGTNACC 1 cut(s) 802
BstENI CCTNNNNNAGG 1 cut(s) 791
BstF5I GGATG 3 cut(s) 62, 130, 942
BstFNI CGCG 7 cut(s) 111, 490, 992, 1025, 1454, 1490, 1526
BstH2I RGCGCY 3 cut(s) 351, 843, 1520
BstHHI GCGC 6 cut(s) 111, 350, 758, 774, 842, 1519
BstKTI GATC 6 cut(s) 280, 451, 813, 891, 1135, 1203
BstMAI GTCTC 3 cut(s) 654, 670, 1415
BstMBI GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
BstMCI CGRYCG 3 cut(s) 415, 1163, 1203
BstNI CCWGG 4 cut(s) 543, 800, 1292, 1334
BstNSI RCATGY 2 cut(s) 1392, 1510
BstPI GGTNACC 1 cut(s) 802
BstSCI CCNGG 9 cut(s) 541, 693, 798, 976, 1192, 1290, 1332, 1404, 1442
BstUI CGCG 7 cut(s) 111, 490, 992, 1025, 1454, 1490, 1526
BstV1I GCAGC 2 cut(s) 118, 1299
BstV2I GAAGAC 3 cut(s) 153, 234, 507
BstX2I RGATCY 2 cut(s) 277, 888
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 2 cut(s) 277, 888
BstZI CGGCCG 2 cut(s) 412, 1160
Bsu15I ATCGAT 2 cut(s) 438, 915
Bsu36I CCTNAGG 1 cut(s) 584
BsuI GTATCC 1 cut(s) 247
BsuTUI ATCGAT 2 cut(s) 438, 915
BtgI CCRYGG 4 cut(s) 51, 739, 1023, 1218
BtgZI GCGATG 1 cut(s) 104
BtsCI GGATG 3 cut(s) 62, 130, 942
BtsI GCAGTG 1 cut(s) 1439
BtsIMutI CAGTG 1 cut(s) 1439
BveI ACCTGC 1 cut(s) 68
CfoI GCGC 6 cut(s) 111, 350, 758, 774, 842, 1519
Cfr10I RCCGGY 3 cut(s) 836, 946, 1296
Cfr42I CCGCGG 1 cut(s) 1026
ClaI ATCGAT 2 cut(s) 438, 915
CpoI CGGWCCG 2 cut(s) 1280, 1402
CseI GACGC 4 cut(s) 715, 1432, 1443, 1460
Csp6I GTAC 3 cut(s) 1102, 1357, 1539
CspAI ACCGGT 1 cut(s) 1296
CspI CGGWCCG 2 cut(s) 1280, 1402
CviQI GTAC 3 cut(s) 1102, 1357, 1539
DdeI CTNAG 4 cut(s) 482, 584, 792, 870
DinI GGCGCC 1 cut(s) 349
DpnI GATC 6 cut(s) 279, 450, 812, 890, 1134, 1202
DpnII GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
DriI GACNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 4 cut(s) 171, 412, 944, 1160
EagI CGGCCG 2 cut(s) 412, 1160
Eam1104I CTCTTC 2 cut(s) 198, 1526
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 2 cut(s) 198, 1526
EciI GGCGGA 2 cut(s) 1453, 1544
Ecl136II GAGCTC 2 cut(s) 529, 1156
EclXI CGGCCG 2 cut(s) 412, 1160
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 3 cut(s) 237, 531, 1158
Eco47I GGWCC 6 cut(s) 1036, 1280, 1294, 1397, 1402, 1440
Eco52I CGGCCG 2 cut(s) 412, 1160
Eco53kI GAGCTC 2 cut(s) 529, 1156
Eco57I CTGAAG 2 cut(s) 603, 1028
Eco81I CCTNAGG 1 cut(s) 584
Eco91I GGTNACC 1 cut(s) 802
EcoICRI GAGCTC 2 cut(s) 529, 1156
EcoNI CCTNNNNNAGG 1 cut(s) 791
EcoO109I RGGNCCY 2 cut(s) 796, 961
EcoO65I GGTNACC 1 cut(s) 802
EcoRII CCWGG 4 cut(s) 541, 798, 1290, 1332
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 3 cut(s) 237, 531, 1158
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 3 cut(s) 654, 670, 1415
FalI AAGNNNNNCTT 2 cut(s) 1358, 1390
FaqI GGGAC 2 cut(s) 229, 1410
FauI CCCGC 6 cut(s) 326, 495, 993, 997, 1023, 1027
FblI GTMKAC 2 cut(s) 284, 708
Fnu4HI GCNGC 9 cut(s) 87, 107, 346, 357, 982, 1023, 1313, 1415, 1527
FokI GGATG 3 cut(s) 49, 137, 949
FriOI GRGCYC 3 cut(s) 237, 531, 1158
Fsp4HI GCNGC 9 cut(s) 87, 107, 346, 357, 982, 1023, 1313, 1415, 1527
FspBI CTAG 3 cut(s) 878, 1073, 1136
GlaI GCGC 6 cut(s) 110, 349, 757, 773, 841, 1518
GluI GCNGC 9 cut(s) 87, 107, 346, 357, 982, 1023, 1313, 1415, 1527
GsaI CCCAGC 4 cut(s) 146, 244, 886, 1072
GsuI CTGGAG 3 cut(s) 314, 1172, 1316
HaeII RGCGCY 3 cut(s) 351, 843, 1520
HgaI GACGC 4 cut(s) 715, 1432, 1443, 1460
HhaI GCGC 6 cut(s) 111, 350, 758, 774, 842, 1519
Hin1I GRCGYC 2 cut(s) 348, 726
Hin6I GCGC 6 cut(s) 109, 348, 756, 772, 840, 1517
HinP1I GCGC 6 cut(s) 109, 348, 756, 772, 840, 1517
HincII GTYRAC 3 cut(s) 285, 687, 709
HindII GTYRAC 3 cut(s) 285, 687, 709
HphI GGTGA 5 cut(s) 166, 229, 357, 814, 1234
Hpy166II GTNNAC 6 cut(s) 285, 424, 687, 709, 739, 1357
Hpy188I TCNGA 9 cut(s) 253, 277, 310, 326, 622, 731, 974, 1036, 1351
Hpy188III TCNNGA 3 cut(s) 224, 1046, 1136
Hpy8I GTNNAC 6 cut(s) 285, 424, 687, 709, 739, 1357
Hpy99I CGWCG 5 cut(s) 116, 119, 665, 1454, 1561
HpyAV CCTTC 2 cut(s) 57, 871
HpyCH4III ACNGT 4 cut(s) 220, 259, 1252, 1280
HpyCH4V TGCA 3 cut(s) 940, 958, 1315
HpyF3I CTNAG 4 cut(s) 482, 584, 792, 870
Hsp92I GRCGYC 2 cut(s) 348, 726
HspAI GCGC 6 cut(s) 109, 348, 756, 772, 840, 1517
KasI GGCGCC 1 cut(s) 347
KroI GCCGGC 2 cut(s) 836, 946
KroNI GCCGGC 2 cut(s) 838, 948
KspI CCGCGG 1 cut(s) 1026
Kzo9I GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
LguI GCTCTTC 1 cut(s) 1526
LmnI GCTCC 6 cut(s) 413, 589, 1153, 1161, 1258, 1471
Lsp1109I GCAGC 2 cut(s) 118, 1299
LweI GCATC 2 cut(s) 927, 1129
MaeI CTAG 3 cut(s) 878, 1073, 1136
MaeIII GTNAC 2 cut(s) 802, 1246
MalI GATC 6 cut(s) 279, 450, 812, 890, 1134, 1202
MbiI CCGCTC 1 cut(s) 1414
MboI GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
MflI RGATCY 2 cut(s) 277, 888
MhlI GDGCHC 3 cut(s) 237, 531, 1158
MluCI AATT 4 cut(s) 327, 520, 778, 1094
MluI ACGCGT 2 cut(s) 1452, 1488
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 7 cut(s) 95, 426, 692, 704, 726, 912, 1316
MmeI TCCRAC 5 cut(s) 189, 679, 997, 1329, 1509
MroNI GCCGGC 2 cut(s) 836, 946
MroXI GAANNNNTTC 1 cut(s) 1083
MseI TTAA 1 cut(s) 1001
MslI CAYNNNNRTG 1 cut(s) 1088
MspA1I CMGCKG 4 cut(s) 244, 359, 886, 1025
MspR9I CCNGG 9 cut(s) 543, 695, 800, 978, 1194, 1292, 1334, 1406, 1444
MvaI CCWGG 4 cut(s) 543, 800, 1292, 1334
MvnI CGCG 7 cut(s) 111, 490, 992, 1025, 1454, 1490, 1526
NaeI GCCGGC 2 cut(s) 838, 948
NarI GGCGCC 1 cut(s) 348
NciI CCSGG 5 cut(s) 695, 978, 1194, 1406, 1444
NcoI CCATGG 1 cut(s) 51
NdeII GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
NgoMIV GCCGGC 2 cut(s) 836, 946
NheI GCTAGC 1 cut(s) 1072
NlaIV GGNNCC 6 cut(s) 349, 409, 591, 1398, 1442, 1483
NmeAIII GCCGAG 3 cut(s) 440, 1168, 1188
NmuCI GTSAC 2 cut(s) 802, 1246
NspI RCATGY 2 cut(s) 1392, 1510
PaeI GCATGC 2 cut(s) 1392, 1510
PciSI GCTCTTC 1 cut(s) 1526
PcsI WCGNNNNNNNCGW 3 cut(s) 281, 444, 1416
PdiI GCCGGC 2 cut(s) 838, 948
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 5 cut(s) 362, 396, 575, 928, 1008
PflFI GACNNNGTC 2 cut(s) 1406, 1426
PfoI TCCNGGA 2 cut(s) 1290, 1332
PinAI ACCGGT 1 cut(s) 1296
PkrI GCNGC 9 cut(s) 88, 108, 347, 358, 983, 1024, 1314, 1416, 1528
Ple19I CGATCG 1 cut(s) 1203
PleI GAGTC 7 cut(s) 95, 425, 691, 704, 726, 911, 1315
PluTI GGCGCC 1 cut(s) 351
PpsI GAGTC 7 cut(s) 95, 425, 691, 704, 726, 911, 1315
Psp124BI GAGCTC 2 cut(s) 531, 1158
Psp6I CCWGG 4 cut(s) 541, 798, 1290, 1332
PspEI GGTNACC 1 cut(s) 802
PspFI CCCAGC 4 cut(s) 142, 240, 882, 1068
PspGI CCWGG 4 cut(s) 541, 798, 1290, 1332
PspN4I GGNNCC 6 cut(s) 349, 409, 591, 1398, 1442, 1483
PsuI RGATCY 2 cut(s) 277, 888
PsyI GACNNNGTC 2 cut(s) 1406, 1426
PvuI CGATCG 1 cut(s) 1203
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 3 cut(s) 1103, 1358, 1540
RsaNI GTAC 3 cut(s) 1102, 1357, 1539
RseI CAYNNNNRTG 1 cut(s) 1088
Rsr2I CGGWCCG 2 cut(s) 1280, 1402
RsrII CGGWCCG 2 cut(s) 1280, 1402
SacI GAGCTC 2 cut(s) 531, 1158
SacII CCGCGG 1 cut(s) 1026
SalI GTCGAC 2 cut(s) 283, 707
SapI GCTCTTC 1 cut(s) 1526
SaqAI TTAA 1 cut(s) 1001
SatI GCNGC 9 cut(s) 87, 107, 346, 357, 982, 1023, 1313, 1415, 1527
Sau3AI GATC 6 cut(s) 277, 448, 810, 888, 1132, 1200
SchI GAGTC 7 cut(s) 95, 426, 692, 704, 726, 912, 1316
ScrFI CCNGG 9 cut(s) 543, 695, 800, 978, 1194, 1292, 1334, 1406, 1444
SduI GDGCHC 3 cut(s) 237, 531, 1158
SfaNI GCATC 2 cut(s) 927, 1129
SfiI GGCCNNNNNGGCC 1 cut(s) 179
SfoI GGCGCC 1 cut(s) 349
Sfr303I CCGCGG 1 cut(s) 1026
SgrBI CCGCGG 1 cut(s) 1026
SinI GGWCC 6 cut(s) 1036, 1280, 1294, 1397, 1402, 1440
SmiMI CAYNNNNRTG 1 cut(s) 1088
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SphI GCATGC 2 cut(s) 1392, 1510
Sse9I AATT 4 cut(s) 327, 520, 778, 1094
SspDI GGCGCC 1 cut(s) 347
SspMI CTAG 3 cut(s) 878, 1073, 1136
SstI GAGCTC 2 cut(s) 531, 1158
StyD4I CCNGG 9 cut(s) 541, 693, 798, 976, 1192, 1290, 1332, 1404, 1442
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 4 cut(s) 220, 259, 1252, 1280
TasI AATT 4 cut(s) 327, 520, 778, 1094
TatI WGTACW 2 cut(s) 1101, 1356
TauI GCSGC 7 cut(s) 89, 348, 359, 984, 1025, 1417, 1529
TfiI GAWTC 5 cut(s) 362, 396, 575, 928, 1008
Tru1I TTAA 1 cut(s) 1001
Tru9I TTAA 1 cut(s) 1001
TscAI CASTG 1 cut(s) 1439
TseFI GTSAC 2 cut(s) 802, 1246
TseI GCWGC 2 cut(s) 106, 1312
Tsp45I GTSAC 2 cut(s) 802, 1246
TspDTI ATGAA 8 cut(s) 17, 199, 251, 476, 941, 1076, 1247, 1463
TspGWI ACGGA 1 cut(s) 1207
TspRI CASTG 1 cut(s) 1439
Tth111I GACNNNGTC 2 cut(s) 1406, 1426
VpaK11BI GGWCC 6 cut(s) 1036, 1280, 1294, 1397, 1402, 1440
XagI CCTNNNNNAGG 1 cut(s) 791
XapI RAATTY 3 cut(s) 327, 778, 1094
XbaI TCTAGA 1 cut(s) 1135
XceI RCATGY 2 cut(s) 1392, 1510
XcmI CCANNNNNNNNNTGG 1 cut(s) 1510
XmiI GTMKAC 2 cut(s) 284, 708
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 3 cut(s) 878, 1073, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.