MD16G1067500.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008776
Physical Location & Seq
Forward (+)
4753746 .. 4756694
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1067500.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGAGCTCCGACGCTTCCGACTCGTCCGGTGGCGGGTGTGCTTTCACGGCCGACAAGGACAAGCAGAAGGAGAAGGCCCGAGTCAGCCGCACCTCCCTCATACTCTGGCACGCCCACCACAACGACGTCGTCGCCGTCCGCAAGCTCCTCGAGGAGGATCAATCCCTCGTCCACGCCCGCGACTACGACAGCCGCACTCCGCTCCACGTCGCCTCGCTCCACGGCTGGATCGACGTCGCCAAGTGCCTTATTGAGTATGGCGCCGATGTCAACGCCCAAGATCGCTGGAAGAACACTCCCCTGGCTGATGCAGAAGGAGCTAAGAAGCCTAAGATGATTGAGCTTCTGAAATCGTACGGTGGCTTGTCTTATGGCCAAAATGGGAGCCATTTCGAACCGAAGCCCGTGCCGCCCCCTCTGCCCAACAAGTGTGATTGGGAGATTGAACCGTCCGAGCTCGACTTCTCCAGCTCACTTACCATTGGGAAGGGCTCTTTTGGCGAAATTTTAAAAGCGCATTGGCGTGGGACACCAGTAGCTGTCAAACGCATTCTTCCATCTCTTTCCGGTGATAGATTGGTGATCCAAGACTTCCGGCATGAAGTTAATTTGCTAGTGAAGCTTCGTCATCCTAATATTGTCCAATTTCTTGGAGCAGTCACTGAGAAGAAGCCCCTTATGCTAATAACGGAGTATTTAAGAGGGGGTGATCTTCATCAGTACCTTAAGGACAAGGGTGCACTTAGTCCTACAACGGCAATTAACTTTGCTTTGGATATTGCCAGAGGCATGGCTTATCTTCACAATGAGCCAAATGTTATAATTCACCGAGACCTAAAACCAAGGAATGTTCTCTTAGTCAATTCTAGTGCCGACCATTTAAAAGTTGGAGATTTTGGATTAAGCAAGCTCATCAAGGTTCAGAATTCTCATGATGTATACAAGATGACTGGCGAAACTGGAAGTTGTGAGTGTCTTCTGAAAAGTTCAATATTGAGTACAAGTTACTATTAA

Protein Analysis

338

Amino Acids

37.35

Weight (kDa)

8.16

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 24 - 94 1.3e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 37 - 119 9e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 49 - 101 6.3e-07 Ankyrin repeats (many copies)
Ank_3 PF13606 64 - 92 7.5e-06 Ankyrin repeat
Ank PF00023 65 - 95 1.6e-07 Ankyrin repeat
Pkinase PF00069 159 - 319 1.6e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 160 - 311 2.2e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
AatII GACGTC 2 cut(s) 129, 237
AbsI CCTCGAGG 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 260
AccBSI CCGCTC 1 cut(s) 202
AccI GTMKAC 1 cut(s) 939
AccII CGCG 1 cut(s) 180
AciI CCGC 7 cut(s) 33, 88, 139, 178, 193, 200, 410
AclWI GGATC 3 cut(s) 165, 236, 577
AcoI YGGCCR 2 cut(s) 48, 373
AcsI RAATTY 2 cut(s) 504, 925
AcyI GRCGYC 3 cut(s) 126, 234, 261
AfaI GTAC 3 cut(s) 356, 722, 1000
AfiI CCNNNNNNNGG 2 cut(s) 33, 154
AflII CTTAAG 1 cut(s) 725
AgsI TTSAA 2 cut(s) 446, 990
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 9 cut(s) 6, 145, 320, 343, 457, 471, 539, 622, 910
AluI AGCT 9 cut(s) 6, 145, 320, 343, 457, 471, 539, 622, 910
Alw21I GWGCWC 3 cut(s) 8, 459, 742
Alw26I GTCTC 1 cut(s) 825
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 3 cut(s) 165, 236, 577
AlwNI CAGNNNCTG 2 cut(s) 539, 662
Ama87I CYCGRG 2 cut(s) 78, 149
AoxI GGCC 3 cut(s) 48, 75, 373
ApaLI GTGCAC 1 cut(s) 738
ApoI RAATTY 2 cut(s) 504, 925
AspLEI GCGC 2 cut(s) 263, 517
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 4 cut(s) 581, 592, 719, 818
AsuII TTCGAA 1 cut(s) 393
AvaI CYCGRG 2 cut(s) 78, 149
BaeGI GKGCMC 1 cut(s) 742
BalI TGGCCA 1 cut(s) 375
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 3 cut(s) 8, 459, 494
BbsI GAAGAC 1 cut(s) 968
Bbv12I GWGCWC 3 cut(s) 8, 459, 742
BccI CCATC 1 cut(s) 565
BceAI ACGGC 4 cut(s) 63, 119, 238, 771
BcgI CGANNNNNNTGC 4 cut(s) 130, 164, 388, 422
BciT130I CCWGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 825
BfaI CTAG 2 cut(s) 614, 867
BfoI RGCGCY 1 cut(s) 264
BfrI CTTAAG 1 cut(s) 725
BisI GCNGC 3 cut(s) 88, 193, 410
BlsI GCNGC 3 cut(s) 89, 194, 411
Bme1390I CCNGG 1 cut(s) 302
BmeT110I CYCGRG 2 cut(s) 78, 149
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 2 cut(s) 262, 386
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 968
BpmI CTGGAG 1 cut(s) 451
Bpu14I TTCGAA 1 cut(s) 393
BsaBI GATNNNNATC 1 cut(s) 714
BsaHI GRCGYC 3 cut(s) 126, 234, 261
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 3 cut(s) 220, 300, 842
BsaWI WCCGGW 2 cut(s) 26, 566
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 154
Bse1I ACTGG 3 cut(s) 533, 955, 964
Bse8I GATNNNNATC 1 cut(s) 714
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 3 cut(s) 220, 300, 842
BseGI GGATG 1 cut(s) 628
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 2 cut(s) 33, 154
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 3 cut(s) 533, 955, 964
BseRI GAGGAG 2 cut(s) 137, 167
BseSI GKGCMC 1 cut(s) 742
BseX3I CGGCCG 1 cut(s) 48
Bsh1236I CGCG 1 cut(s) 180
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 50, 77, 375
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 3 cut(s) 8, 459, 742
BsiHKCI CYCGRG 2 cut(s) 78, 149
BsiSI CCGG 3 cut(s) 27, 567, 595
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 33, 154
BsmAI GTCTC 1 cut(s) 825
BsmFI GGGAC 1 cut(s) 541
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 3 cut(s) 50, 77, 375
Bso31I GGTCTC 1 cut(s) 825
BsoBI CYCGRG 2 cut(s) 78, 149
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 4 cut(s) 8, 459, 494, 742
Bsp143I GATC 5 cut(s) 157, 228, 280, 582, 709
BspACI CCGC 7 cut(s) 33, 88, 139, 178, 193, 200, 410
BspANI GGCC 3 cut(s) 50, 77, 375
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 1 cut(s) 180
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 2 cut(s) 262, 386
BspPI GGATC 3 cut(s) 165, 236, 577
BspT104I TTCGAA 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 260
BspTI CTTAAG 1 cut(s) 725
BspTNI GGTCTC 1 cut(s) 825
BsrBI CCGCTC 1 cut(s) 202
BsrI ACTGG 3 cut(s) 533, 955, 964
BssECI CCNNGG 3 cut(s) 220, 300, 842
BssMI GATC 5 cut(s) 157, 228, 280, 582, 709
BssNAI GTATAC 1 cut(s) 940
BssNI GRCGYC 3 cut(s) 126, 234, 261
BssT1I CCWWGG 1 cut(s) 842
Bst1107I GTATAC 1 cut(s) 940
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 359, 450
BstACI GRCGYC 3 cut(s) 126, 234, 261
BstAFI CTTAAG 1 cut(s) 725
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 4 cut(s) 111, 143, 178, 908
BstDEI CTNAG 5 cut(s) 321, 330, 663, 743, 856
BstDSI CCRYGG 1 cut(s) 220
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 1 cut(s) 628
BstFNI CGCG 1 cut(s) 180
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 2 cut(s) 263, 517
BstKTI GATC 5 cut(s) 160, 231, 283, 585, 712
BstMAI GTCTC 1 cut(s) 825
BstMBI GATC 5 cut(s) 157, 228, 280, 582, 709
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 7 cut(s) 47, 317, 409, 418, 498, 619, 679
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 742
BstUI CGCG 1 cut(s) 180
BstV2I GAAGAC 1 cut(s) 968
BstXI CCANNNNNNTGG 2 cut(s) 650, 790
BstZ17I GTATAC 1 cut(s) 940
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 50, 77, 375
BtgI CCRYGG 1 cut(s) 220
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 628
BtsIMutI CAGTG 1 cut(s) 660
Cac8I GCNNGC 4 cut(s) 111, 143, 178, 908
CaiI CAGNNNCTG 2 cut(s) 539, 662
CciI TCATGA 1 cut(s) 931
CfoI GCGC 2 cut(s) 263, 517
Cfr13I GGNCC 1 cut(s) 76
CseI GACGC 1 cut(s) 20
Csp6I GTAC 3 cut(s) 355, 721, 999
CviAII CATG 3 cut(s) 599, 790, 932
CviQI GTAC 3 cut(s) 355, 721, 999
DdeI CTNAG 5 cut(s) 321, 330, 663, 743, 856
DinI GGCGCC 1 cut(s) 262
DpnI GATC 5 cut(s) 159, 230, 282, 584, 711
DpnII GATC 5 cut(s) 157, 228, 280, 582, 709
DraI TTTAAA 2 cut(s) 510, 882
EaeI YGGCCR 2 cut(s) 48, 373
EagI CGGCCG 1 cut(s) 48
Ecl136II GAGCTC 2 cut(s) 6, 457
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 842
Eco24I GRGCYC 3 cut(s) 8, 459, 494
Eco31I GGTCTC 1 cut(s) 825
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 2 cut(s) 6, 457
Eco88I CYCGRG 2 cut(s) 78, 149
EcoICRI GAGCTC 2 cut(s) 6, 457
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 925
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 842
EcoT38I GRGCYC 3 cut(s) 8, 459, 494
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 842
FaeI CATG 3 cut(s) 602, 793, 935
FaiI YATR 9 cut(s) 101, 258, 372, 600, 680, 791, 821, 933, 940
FaqI GGGAC 1 cut(s) 541
FatI CATG 3 cut(s) 598, 789, 931
FauI CCCGC 2 cut(s) 26, 185
FblI GTMKAC 1 cut(s) 939
Fnu4HI GCNGC 3 cut(s) 88, 193, 410
FokI GGATG 1 cut(s) 615
FriOI GRGCYC 3 cut(s) 8, 459, 494
Fsp4HI GCNGC 3 cut(s) 88, 193, 410
FspBI CTAG 2 cut(s) 614, 867
GlaI GCGC 2 cut(s) 262, 516
GluI GCNGC 3 cut(s) 88, 193, 410
GsuI CTGGAG 1 cut(s) 451
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 3 cut(s) 50, 77, 375
HapII CCGG 3 cut(s) 27, 567, 595
HgaI GACGC 1 cut(s) 20
HhaI GCGC 2 cut(s) 263, 517
Hin1I GRCGYC 3 cut(s) 126, 234, 261
Hin1II CATG 3 cut(s) 602, 793, 935
Hin6I GCGC 2 cut(s) 261, 515
HinP1I GCGC 2 cut(s) 261, 515
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 2 cut(s) 20, 81
HpaII CCGG 3 cut(s) 27, 567, 595
HphI GGTGA 4 cut(s) 581, 592, 719, 818
Hpy166II GTNNAC 4 cut(s) 172, 271, 740, 940
Hpy188I TCNGA 6 cut(s) 10, 19, 348, 454, 924, 981
Hpy188III TCNNGA 1 cut(s) 932
Hpy8I GTNNAC 4 cut(s) 172, 271, 740, 940
Hpy99I CGWCG 7 cut(s) 14, 128, 131, 134, 212, 236, 239
HpyAV CCTTC 4 cut(s) 61, 67, 308, 481
HpyCH4III ACNGT 2 cut(s) 359, 450
HpyCH4IV ACGT 3 cut(s) 126, 207, 234
HpyCH4V TGCA 2 cut(s) 311, 740
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 317, 409, 418, 498, 619, 679
HpyF3I CTNAG 5 cut(s) 321, 330, 663, 743, 856
HpySE526I ACGT 3 cut(s) 126, 207, 234
Hsp92I GRCGYC 3 cut(s) 126, 234, 261
Hsp92II CATG 3 cut(s) 602, 793, 935
HspAI GCGC 2 cut(s) 261, 515
KasI GGCGCC 1 cut(s) 260
Kzo9I GATC 5 cut(s) 157, 228, 280, 582, 709
LmnI GCTCC 7 cut(s) 11, 150, 207, 222, 317, 384, 653
LweI GCATC 1 cut(s) 298
MaeI CTAG 2 cut(s) 614, 867
MaeII ACGT 3 cut(s) 126, 207, 234
MaeIII GTNAC 2 cut(s) 658, 1004
MalI GATC 5 cut(s) 159, 230, 282, 584, 711
MbiI CCGCTC 1 cut(s) 202
MboI GATC 5 cut(s) 157, 228, 280, 582, 709
MboII GAAGA 6 cut(s) 301, 545, 679, 704, 791, 968
MhlI GDGCHC 4 cut(s) 8, 459, 494, 742
MlsI TGGCCA 1 cut(s) 375
MluCI AATT 7 cut(s) 504, 607, 644, 759, 822, 862, 925
MluNI TGGCCA 1 cut(s) 375
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 2 cut(s) 14, 90
MmeI TCCRAC 3 cut(s) 33, 42, 868
Mox20I TGGCCA 1 cut(s) 375
MscI TGGCCA 1 cut(s) 375
MseI TTAA 8 cut(s) 509, 606, 698, 726, 762, 881, 902, 1012
MslI CAYNNNNRTG 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 375
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 3 cut(s) 27, 567, 595
MspR9I CCNGG 1 cut(s) 302
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 7 cut(s) 47, 317, 409, 418, 498, 619, 679
NarI GGCGCC 1 cut(s) 261
NdeII GATC 5 cut(s) 157, 228, 280, 582, 709
NlaIII CATG 3 cut(s) 602, 793, 935
NlaIV GGNNCC 2 cut(s) 262, 386
NmuCI GTSAC 1 cut(s) 658
NspV TTCGAA 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 931
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 228
PctI GAATGC 1 cut(s) 549
Pfl23II CGTACG 1 cut(s) 354
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 3 cut(s) 89, 194, 411
PleI GAGTC 2 cut(s) 14, 89
PluTI GGCGCC 1 cut(s) 264
PpsI GAGTC 2 cut(s) 14, 89
PsiI TTATAA 1 cut(s) 821
Psp124BI GAGCTC 2 cut(s) 8, 459
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspLI CGTACG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 262, 386
PspPI GGNCC 1 cut(s) 76
PspXI VCTCGAGB 1 cut(s) 149
PstNI CAGNNNCTG 2 cut(s) 539, 662
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 3 cut(s) 356, 722, 1000
RsaNI GTAC 3 cut(s) 355, 721, 999
RseI CAYNNNNRTG 1 cut(s) 522
SacI GAGCTC 2 cut(s) 8, 459
SaqAI TTAA 8 cut(s) 509, 606, 698, 726, 762, 881, 902, 1012
SatI GCNGC 3 cut(s) 88, 193, 410
Sau3AI GATC 5 cut(s) 157, 228, 280, 582, 709
Sau96I GGNCC 1 cut(s) 76
SchI GAGTC 2 cut(s) 14, 90
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 4 cut(s) 8, 459, 494, 742
SfaNI GCATC 1 cut(s) 298
SfoI GGCGCC 1 cut(s) 262
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 393
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 2 cut(s) 149, 725
SmoI CTYRAG 2 cut(s) 149, 725
Sse9I AATT 7 cut(s) 504, 607, 644, 759, 822, 862, 925
SsiI CCGC 7 cut(s) 33, 88, 139, 178, 193, 200, 410
SspDI GGCGCC 1 cut(s) 260
SspI AATATT 2 cut(s) 637, 993
SspMI CTAG 2 cut(s) 614, 867
SstI GAGCTC 2 cut(s) 8, 459
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 842
TaaI ACNGT 2 cut(s) 359, 450
TaiI ACGT 3 cut(s) 129, 210, 237
TaqI TCGA 4 cut(s) 150, 231, 393, 459
TasI AATT 7 cut(s) 504, 607, 644, 759, 822, 862, 925
TatI WGTACW 1 cut(s) 998
TauI GCSGC 3 cut(s) 90, 195, 412
Tru1I TTAA 8 cut(s) 509, 606, 698, 726, 762, 881, 902, 1012
Tru9I TTAA 8 cut(s) 509, 606, 698, 726, 762, 881, 902, 1012
TscAI CASTG 1 cut(s) 667
TseFI GTSAC 1 cut(s) 658
Tsp45I GTSAC 1 cut(s) 658
TspDTI ATGAA 2 cut(s) 615, 704
TspGWI ACGGA 1 cut(s) 704
TspRI CASTG 1 cut(s) 667
Tth111I GACNNNGTC 1 cut(s) 128
Vha464I CTTAAG 1 cut(s) 725
VneI GTGCAC 1 cut(s) 738
XagI CCTNNNNNAGG 1 cut(s) 152
XapI RAATTY 2 cut(s) 504, 925
XcmI CCANNNNNNNNNTGG 1 cut(s) 884
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 939
XspI CTAG 2 cut(s) 614, 867
ZraI GACGTC 2 cut(s) 127, 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.