RchiOBHm_Chr4g0439171
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008776
Physical Location & Seq
Forward (+)
61606912 .. 61610918
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40724

Sequence Viewer

Length: 1266 bp
ATGAGCTCCTCCTCCGACGCCTCCGAATCGTCGGGAGCTAAGCCGTGCGCGGCGACGGCCGACAAGGACAAGCAGAAGGAGAAGGCGAGAGTGAGCCGCACCTCGCTCATACTCTGGCACGCTCACCACAACGACGCCGTCGCAGTCCGCAAGCTTCTCGAGGAGGATCGCGCCCTCGTTCACGCCAGAGACTACGACAACCGCACCCCGCTCCACGTCGCCTCCCTCCACGGCTGGATCGACGTCGCCAAATGCCTCATCGAGTACGGCGCCGATGTCAACGCCCAGGATCGTTGGAAGAACACTCCTCTAGCTGATGCTGAAGGAGCTAAGAAGCACATCATGATTGAGCTCTTGAAATCTTATGGTGGCTTGTCTTATGGCCAAAATGGGAGCCATTTCGAACCAAAGCCGGTGGCGCCTCCTCTGCCAAACAAGTGTGATTGGGAAATTGAGCCTGCTGAGCTTGACTTCTCCGGATCAGTTACCATCGGGAAGGGCTCGTTTGGTGAGATTTTAAAAGCACATTGGCGTGGAACACCAGTAGCTGTCAAACGCATTCTTCCATCTCTTTCTGGTGATAGATTAGTGATTCAGGACTTCCGGCATGAAGTAAATTTGCTAGTGAAGCTTCGCCACCCTAATATAGTCCAATTTCTTGGAGCTGTCACAGAGAAGAAGCCTCTTATGTTAATTACGGAGTATTTAAGAGGGGGAGATCTTCATCAGTACCTTAAGGAAAAGGGTTCACTTAGTCCTACAACAGCTGTCAGCTTTGCCTTAGACATTGCAAGAGGGATGGCTTATCTTCACAATGAGCCAAATGTTATAATTCATCGTGACCTAAAACCAAGGAATGTTCTTCTAGTCAATTCCAGTGCAGACCATCTCAAAGTTGGAGATTTTGGACTAAGCAAGCTCATCAAGGTTCAAAATTCTCATGATGTGTACAAAATGACTGGCGAAACTGGAAGTTACCGATATATGGCTCCTGAAGTTTTTAAGCACCGGCGATATGATAAGAAGGTTGATGTGTTCTCTTTTGCAATGATACTGTACGAGATGCTCGAAGGAGATCCACCACTTTCAAATTACGAGCCTTATGATGCAGCCAAATTTGTGGCAGAAGGACACAGGCCAATGTTTCATGGAAAAGGACACACCCCTGAACTGAAAGAGTTAATAGAGCAATGCTGGTCTGCTGACATGAACCAAAGACCTTCTTTCTTGGAAATTCTGAAAAAGCTTGAGAAGGTACATAGTTGA

Protein Analysis

421

Amino Acids

47.28

Weight (kDa)

8.16

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 32 - 97 7.9e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 52 - 104 4.8e-06 Ankyrin repeats (many copies)
Ank PF00023 66 - 98 9.5e-08 Ankyrin repeat
Ank_3 PF13606 66 - 95 4e-06 Ankyrin repeat
Pkinase PF00069 162 - 414 1.1e-57 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 163 - 416 9.4e-65 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AatII GACGTC 1 cut(s) 246
AccB1I GGYRCC 2 cut(s) 269, 418
AccBSI CCGCTC 1 cut(s) 211
AccII CGCG 2 cut(s) 50, 171
AccIII TCCGGA 1 cut(s) 476
AciI CCGC 5 cut(s) 50, 97, 148, 202, 209
AclWI GGATC 5 cut(s) 174, 245, 297, 487, 1070
AcoI YGGCCR 2 cut(s) 57, 382
AcsI RAATTY 4 cut(s) 616, 934, 1115, 1233
AcuI CTGAAG 2 cut(s) 342, 1014
AcyI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
AfaI GTAC 5 cut(s) 266, 731, 950, 1058, 1257
AfiI CCNNNNNNNGG 1 cut(s) 985
AflII CTTAAG 1 cut(s) 734
AgsI TTSAA 3 cut(s) 358, 932, 1089
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 285
Alw21I GWGCWC 2 cut(s) 8, 354
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 5 cut(s) 174, 245, 297, 487, 1070
AlwNI CAGNNNCTG 1 cut(s) 548
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 476
AoxI GGCC 3 cut(s) 57, 382, 1136
ApeKI GCWGC 1 cut(s) 1109
ApoI RAATTY 4 cut(s) 616, 934, 1115, 1233
AspLEI GCGC 4 cut(s) 50, 173, 272, 421
AsuHPI GGTGA 3 cut(s) 116, 521, 590
AsuII TTCGAA 1 cut(s) 402
AvaI CYCGRG 1 cut(s) 158
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 2 cut(s) 269, 418
BanII GRGCYC 3 cut(s) 8, 354, 503
Bbv12I GWGCWC 2 cut(s) 8, 354
BbvI GCAGC 1 cut(s) 1121
BccI CCATC 4 cut(s) 497, 574, 793, 894
BceAI ACGGC 5 cut(s) 28, 72, 122, 247, 283
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciT130I CCWGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 3 cut(s) 311, 623, 866
BfoI RGCGCY 2 cut(s) 273, 422
BfrI CTTAAG 1 cut(s) 734
BglII AGATCT 1 cut(s) 718
BisI GCNGC 3 cut(s) 51, 97, 1110
BlpI GCTNAGC 2 cut(s) 39, 462
BlsI GCNGC 3 cut(s) 52, 98, 1111
Bme1390I CCNGG 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 217
BmiI GGNNCC 4 cut(s) 271, 395, 420, 990
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 307, 1053, 1096
Bpu1102I GCTNAGC 2 cut(s) 39, 462
Bpu14I TTCGAA 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 723
BsaHI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
BsaJI CCNNGG 3 cut(s) 229, 285, 851
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 1 cut(s) 985
Bse118I RCCGGY 2 cut(s) 412, 1008
Bse1I ACTGG 4 cut(s) 542, 876, 964, 973
Bse3DI GCAATG 3 cut(s) 786, 1053, 1196
Bse8I GATNNNNATC 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 476
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 3 cut(s) 229, 285, 851
BseGI GGATG 1 cut(s) 804
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 1 cut(s) 985
BseMI GCAATG 3 cut(s) 786, 1053, 1196
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 4 cut(s) 542, 876, 964, 973
BseRI GAGGAG 3 cut(s) 176, 297, 414
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 1 cut(s) 1121
BsgI GTGCAG 1 cut(s) 900
Bsh1236I CGCG 2 cut(s) 50, 171
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 3 cut(s) 59, 384, 1138
BshNI GGYRCC 2 cut(s) 269, 418
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 8, 354
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 4 cut(s) 413, 477, 604, 1009
BslI CCNNNNNNNGG 1 cut(s) 985
BsmAI GTCTC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 3 cut(s) 59, 384, 1138
BsoBI CYCGRG 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 402
Bsp1286I GDGCHC 3 cut(s) 8, 354, 503
Bsp13I TCCGGA 1 cut(s) 476
Bsp1407I TGTACA 1 cut(s) 948
Bsp143I GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
Bsp1720I GCTNAGC 2 cut(s) 39, 462
BspACI CCGC 5 cut(s) 50, 97, 148, 202, 209
BspANI GGCC 3 cut(s) 59, 384, 1138
BspCNI CTCAG 1 cut(s) 454
BspEI TCCGGA 1 cut(s) 476
BspFNI CGCG 2 cut(s) 50, 171
BspHI TCATGA 2 cut(s) 342, 940
BspLI GGNNCC 4 cut(s) 271, 395, 420, 990
BspPI GGATC 5 cut(s) 174, 245, 297, 487, 1070
BspT104I TTCGAA 1 cut(s) 402
BspT107I GGYRCC 2 cut(s) 269, 418
BspTI CTTAAG 1 cut(s) 734
BsrBI CCGCTC 1 cut(s) 211
BsrDI GCAATG 3 cut(s) 786, 1053, 1196
BsrFI RCCGGY 2 cut(s) 412, 1008
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 4 cut(s) 542, 876, 964, 973
BssAI RCCGGY 2 cut(s) 412, 1008
BssECI CCNNGG 3 cut(s) 229, 285, 851
BssMI GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
BssNI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
BssT1I CCWWGG 1 cut(s) 851
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 1056
BstACI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
BstAFI CTTAAG 1 cut(s) 734
BstAUI TGTACA 1 cut(s) 948
BstBI TTCGAA 1 cut(s) 402
BstC8I GCNNGC 4 cut(s) 120, 152, 459, 917
BstDEI CTNAG 6 cut(s) 39, 330, 462, 752, 781, 911
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 804
BstFNI CGCG 2 cut(s) 50, 171
BstH2I RGCGCY 2 cut(s) 273, 422
BstHHI GCGC 4 cut(s) 50, 173, 272, 421
BstKTI GATC 6 cut(s) 169, 240, 292, 482, 721, 1078
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 6 cut(s) 56, 326, 418, 427, 463, 628
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstUI CGCG 2 cut(s) 50, 171
BstV1I GCAGC 1 cut(s) 1121
BstX2I RGATCY 2 cut(s) 718, 1075
BstXI CCANNNNNNTGG 2 cut(s) 659, 1120
BstYI RGATCY 2 cut(s) 718, 1075
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 3 cut(s) 59, 384, 1138
BtgI CCRYGG 1 cut(s) 229
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 1 cut(s) 804
BtsIMutI CAGTG 1 cut(s) 883
Cac8I GCNNGC 4 cut(s) 120, 152, 459, 917
CaiI CAGNNNCTG 1 cut(s) 548
CciI TCATGA 2 cut(s) 342, 940
CfoI GCGC 4 cut(s) 50, 173, 272, 421
Cfr10I RCCGGY 2 cut(s) 412, 1008
CseI GACGC 2 cut(s) 26, 143
Csp6I GTAC 5 cut(s) 265, 730, 949, 1057, 1256
CspCI CAANNNNNGTGG 2 cut(s) 396, 431
CviAII CATG 5 cut(s) 343, 608, 941, 1148, 1207
CviQI GTAC 5 cut(s) 265, 730, 949, 1057, 1256
DdeI CTNAG 6 cut(s) 39, 330, 462, 752, 781, 911
DinI GGCGCC 2 cut(s) 271, 420
DpnI GATC 6 cut(s) 168, 239, 291, 481, 720, 1077
DpnII GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 2 cut(s) 57, 382
EagI CGGCCG 1 cut(s) 57
Ecl136II GAGCTC 2 cut(s) 6, 352
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 851
Eco24I GRGCYC 3 cut(s) 8, 354, 503
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 2 cut(s) 6, 352
Eco57I CTGAAG 2 cut(s) 342, 1014
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 2 cut(s) 6, 352
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 851
EcoT38I GRGCYC 3 cut(s) 8, 354, 503
EgeI GGCGCC 2 cut(s) 271, 420
EheI GGCGCC 2 cut(s) 271, 420
ErhI CCWWGG 1 cut(s) 851
FaeI CATG 5 cut(s) 346, 611, 944, 1151, 1210
FalI AAGNNNNNCTT 2 cut(s) 1207, 1239
FatI CATG 5 cut(s) 342, 607, 940, 1147, 1206
FauI CCCGC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 51, 97, 1110
FokI GGATG 1 cut(s) 811
FriOI GRGCYC 3 cut(s) 8, 354, 503
Fsp4HI GCNGC 3 cut(s) 51, 97, 1110
FspBI CTAG 3 cut(s) 311, 623, 866
GlaI GCGC 4 cut(s) 49, 172, 271, 420
GluI GCNGC 3 cut(s) 51, 97, 1110
HaeII RGCGCY 2 cut(s) 273, 422
HaeIII GGCC 3 cut(s) 59, 384, 1138
HapII CCGG 4 cut(s) 413, 477, 604, 1009
HgaI GACGC 2 cut(s) 26, 143
HhaI GCGC 4 cut(s) 50, 173, 272, 421
Hin1I GRCGYC 5 cut(s) 18, 135, 243, 270, 419
Hin1II CATG 5 cut(s) 346, 611, 944, 1151, 1210
Hin6I GCGC 4 cut(s) 48, 171, 270, 419
HinP1I GCGC 4 cut(s) 48, 171, 270, 419
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 3 cut(s) 152, 629, 1244
HinfI GANTC 2 cut(s) 26, 592
HpaII CCGG 4 cut(s) 413, 477, 604, 1009
HphI GGTGA 3 cut(s) 116, 521, 590
Hpy166II GTNNAC 4 cut(s) 181, 280, 749, 949
Hpy188I TCNGA 3 cut(s) 16, 25, 1239
Hpy8I GTNNAC 4 cut(s) 181, 280, 749, 949
Hpy99I CGWCG 8 cut(s) 20, 34, 58, 137, 143, 221, 245, 248
HpyAV CCTTC 9 cut(s) 70, 76, 317, 490, 1018, 1064, 1121, 1230, 1246
HpyCH4III ACNGT 1 cut(s) 1056
HpyCH4IV ACGT 2 cut(s) 216, 243
HpyCH4V TGCA 4 cut(s) 791, 881, 1046, 1109
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 326, 418, 427, 463, 628
HpyF3I CTNAG 6 cut(s) 39, 330, 462, 752, 781, 911
HpySE526I ACGT 2 cut(s) 216, 243
Hsp92I GRCGYC 5 cut(s) 18, 135, 243, 270, 419
Hsp92II CATG 5 cut(s) 346, 611, 944, 1151, 1210
HspAI GCGC 4 cut(s) 48, 171, 270, 419
KasI GGCGCC 2 cut(s) 269, 418
Kpn2I TCCGGA 1 cut(s) 476
Kzo9I GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
LmnI GCTCC 7 cut(s) 11, 35, 216, 326, 393, 662, 994
Lsp1109I GCAGC 1 cut(s) 1121
LweI GCATC 3 cut(s) 307, 1053, 1096
MaeI CTAG 3 cut(s) 311, 623, 866
MaeII ACGT 2 cut(s) 216, 243
MaeIII GTNAC 4 cut(s) 484, 667, 839, 974
MalI GATC 6 cut(s) 168, 239, 291, 481, 720, 1077
MbiI CCGCTC 1 cut(s) 211
MboI GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
MboII GAAGA 6 cut(s) 310, 554, 688, 713, 800, 854
MflI RGATCY 2 cut(s) 718, 1075
MhlI GDGCHC 3 cut(s) 8, 354, 503
MlsI TGGCCA 1 cut(s) 384
MluNI TGGCCA 1 cut(s) 384
Mly113I GGCGCC 2 cut(s) 270, 419
MmeI TCCRAC 3 cut(s) 39, 275, 877
Mox20I TGGCCA 1 cut(s) 384
MroI TCCGGA 1 cut(s) 476
MscI TGGCCA 1 cut(s) 384
MseI TTAA 6 cut(s) 518, 692, 707, 735, 1002, 1181
MslI CAYNNNNRTG 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 767
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 4 cut(s) 413, 477, 604, 1009
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 2 cut(s) 50, 171
MwoI GCNNNNNNNGC 6 cut(s) 56, 326, 418, 427, 463, 628
NarI GGCGCC 2 cut(s) 270, 419
NdeII GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
NlaIII CATG 5 cut(s) 346, 611, 944, 1151, 1210
NlaIV GGNNCC 4 cut(s) 271, 395, 420, 990
NmuCI GTSAC 2 cut(s) 667, 839
NspV TTCGAA 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 2 cut(s) 342, 940
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 1065
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 26, 592
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 52, 98, 1111
PluTI GGCGCC 2 cut(s) 273, 422
PsiI TTATAA 1 cut(s) 830
Psp124BI GAGCTC 2 cut(s) 8, 354
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 4 cut(s) 271, 395, 420, 990
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 2 cut(s) 718, 1075
PsyI GACNNNGTC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 5 cut(s) 266, 731, 950, 1058, 1257
RsaNI GTAC 5 cut(s) 265, 730, 949, 1057, 1256
RseI CAYNNNNRTG 1 cut(s) 531
SacI GAGCTC 2 cut(s) 8, 354
SaqAI TTAA 6 cut(s) 518, 692, 707, 735, 1002, 1181
SatI GCNGC 3 cut(s) 51, 97, 1110
Sau3AI GATC 6 cut(s) 166, 237, 289, 479, 718, 1075
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 3 cut(s) 8, 354, 503
SfaNI GCATC 3 cut(s) 307, 1053, 1096
SfoI GGCGCC 2 cut(s) 271, 420
Sfr274I CTCGAG 1 cut(s) 158
SfuI TTCGAA 1 cut(s) 402
SgrAI CRCCGGYG 1 cut(s) 1008
SlaI CTCGAG 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 3 cut(s) 158, 734, 1247
SmoI CTYRAG 3 cut(s) 158, 734, 1247
SsiI CCGC 5 cut(s) 50, 97, 148, 202, 209
SspDI GGCGCC 2 cut(s) 269, 418
SspMI CTAG 3 cut(s) 311, 623, 866
SstI GAGCTC 2 cut(s) 8, 354
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 1 cut(s) 851
TaaI ACNGT 1 cut(s) 1056
TaiI ACGT 2 cut(s) 219, 246
TaqI TCGA 5 cut(s) 159, 240, 261, 402, 1068
TatI WGTACW 1 cut(s) 948
TauI GCSGC 2 cut(s) 53, 99
TfiI GAWTC 2 cut(s) 26, 592
Tru1I TTAA 6 cut(s) 518, 692, 707, 735, 1002, 1181
Tru9I TTAA 6 cut(s) 518, 692, 707, 735, 1002, 1181
TscAI CASTG 1 cut(s) 883
TseFI GTSAC 2 cut(s) 667, 839
TseI GCWGC 1 cut(s) 1109
Tsp45I GTSAC 2 cut(s) 667, 839
TspDTI ATGAA 5 cut(s) 624, 713, 824, 1136, 1223
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 1 cut(s) 883
Tth111I GACNNNGTC 1 cut(s) 137
Vha464I CTTAAG 1 cut(s) 734
XapI RAATTY 4 cut(s) 616, 934, 1115, 1233
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XhoI CTCGAG 1 cut(s) 158
XspI CTAG 3 cut(s) 311, 623, 866
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.