Rh4BG378800
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008776
Physical Location & Seq
Forward (+)
53359320 .. 53364044
4725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG378800.1

Sequence Viewer

Length: 1125 bp
ATGAGCTCCTCCTCCGACGCCTCCGACTCGTCGGGAGCTAAGCCGTGCGCGGCGACGGCCGACAAGGACAAGCAGAAGGAGAAGGCGAGAGTGAGCCGCACCTCCCTCATACTCTGGCACGCTCACCACAACGACGCCGTCGCAGTCCGCAAGCTTCTCGAGGAGGATCGCGCCCTCGTTCACGCCAGAGACTACGACAACCGCACTCCGCTCCACGTCGCCTCCCTCCACGGCTGGATCGACGTCGCCAAATGCCTCATCGAGTACGGCGCCGATGTCAACGCCCAGGATCGTTGGAAGAACACTCCTCTAGCTGATGCCGAAGGAGCTAAGAAGCACACCATGATTGAGCTGTTGAAATCTTATGGTGGCTTGTCTTATGGCCAAAATGGGAGCCATTTCGAACCGAAGCCGGTGGCGCCTCCTCTGCCAAACAAGTGTGATTGGGAAATTGAGCCTGCTGAGCTTGACTTCTCCGGATCAGTTACCATCGGGAAGGGCTCGTTTGGTGAGATTTTAAAAGCACATTGGCGTGGAACACCAGTAGCTGTCAAACGCATTCTTCCATCTCTTTCTGGTGATAGATTAGTGATTCAGGACTTCCGGCATGAAGTAAATTTGCTAGTGAAGCTTCGCCACCCTAATATAGTCCAATTTCTTGGAGCTGTAACAGAGAAGAAGCCTCTTATGTTAATTACGGAGTATTTAAGAGGGGGAGATCTTCATCAGTACCTTAAGGAAAAGGGTTCACTTAGTCCTACAACAGCTGTCAGCTTTGCCTTAGACATTGCAAGAGGGATGGCTTATCTTCACAATGAGCCAAATGTTATAATTCATCGTGACCTAAAACCAAGGAATGTTCTTTTAGTCAATTCCAGTGCAGACCATCTCAAAGTTGGAGATTTTGGACTAAGCAAGCTCATCAAGGTTCAAAATTCTCATGATGTGTACAAAATGACTGGCGAAACTGGAAGTTACCGATATATGGCTCCCGAAGTTTTTAAGCACCGGCGATATGATAAGAAGGTTGATGTGTTCTCTTTTGCAATGATACTGTACGAGGTAATGCTGCTTAGTGCAAGATGTAAAAATCAGATTCGCCTTTCAAAAAAAAAAAAAAAGTGA

Protein Analysis

374

Amino Acids

41.91

Weight (kDa)

9.38

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 27 - 97 9.2e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 40 - 122 1.3e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 52 - 104 4.1e-06 Ankyrin repeats (many copies)
Ank PF00023 66 - 98 8.1e-08 Ankyrin repeat
Ank_3 PF13606 66 - 95 3.4e-06 Ankyrin repeat
Pkinase PF00069 162 - 365 6.7e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 163 - 367 5.6e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AatII GACGTC 1 cut(s) 246
AccB1I GGYRCC 2 cut(s) 269, 418
AccBSI CCGCTC 1 cut(s) 211
AccII CGCG 2 cut(s) 50, 171
AccIII TCCGGA 1 cut(s) 476
AciI CCGC 5 cut(s) 50, 97, 148, 202, 209
AclWI GGATC 4 cut(s) 174, 245, 297, 487
AcoI YGGCCR 2 cut(s) 57, 382
AcsI RAATTY 2 cut(s) 616, 934
AcyI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
AfaI GTAC 4 cut(s) 266, 731, 950, 1058
AfiI CCNNNNNNNGG 1 cut(s) 985
AflII CTTAAG 1 cut(s) 734
AgsI TTSAA 3 cut(s) 358, 932, 1107
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 285
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 4 cut(s) 174, 245, 297, 487
AlwNI CAGNNNCTG 1 cut(s) 548
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 476
AoxI GGCC 2 cut(s) 57, 382
ApeKI GCWGC 1 cut(s) 1069
ApoI RAATTY 2 cut(s) 616, 934
AspLEI GCGC 4 cut(s) 50, 173, 272, 421
AsuHPI GGTGA 3 cut(s) 116, 521, 590
AsuII TTCGAA 1 cut(s) 402
AvaI CYCGRG 1 cut(s) 158
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 2 cut(s) 269, 418
BanII GRGCYC 2 cut(s) 8, 503
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 1056
BccI CCATC 4 cut(s) 497, 574, 793, 894
BceAI ACGGC 5 cut(s) 28, 72, 122, 247, 283
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciT130I CCWGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 2 cut(s) 311, 623
BfoI RGCGCY 2 cut(s) 273, 422
BfrI CTTAAG 1 cut(s) 734
BglII AGATCT 1 cut(s) 718
BisI GCNGC 3 cut(s) 51, 97, 1070
BlpI GCTNAGC 2 cut(s) 39, 462
BlsI GCNGC 3 cut(s) 52, 98, 1071
Bme1390I CCNGG 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 217
BmiI GGNNCC 4 cut(s) 271, 395, 420, 990
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 307
Bpu1102I GCTNAGC 2 cut(s) 39, 462
Bpu14I TTCGAA 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 723
BsaHI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
BsaJI CCNNGG 3 cut(s) 229, 285, 851
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 1 cut(s) 985
Bse118I RCCGGY 2 cut(s) 412, 1008
Bse1I ACTGG 4 cut(s) 542, 876, 964, 973
Bse3DI GCAATG 2 cut(s) 786, 1053
Bse8I GATNNNNATC 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 476
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 3 cut(s) 229, 285, 851
BseGI GGATG 1 cut(s) 804
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 1 cut(s) 985
BseMI GCAATG 2 cut(s) 786, 1053
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 4 cut(s) 542, 876, 964, 973
BseRI GAGGAG 3 cut(s) 176, 297, 414
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 1 cut(s) 1056
BsgI GTGCAG 1 cut(s) 900
Bsh1236I CGCG 2 cut(s) 50, 171
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 384
BshNI GGYRCC 2 cut(s) 269, 418
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 4 cut(s) 413, 477, 604, 1009
BslI CCNNNNNNNGG 1 cut(s) 985
BsmAI GTCTC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 2 cut(s) 59, 384
BsoBI CYCGRG 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 402
Bsp1286I GDGCHC 2 cut(s) 8, 503
Bsp13I TCCGGA 1 cut(s) 476
Bsp1407I TGTACA 1 cut(s) 948
Bsp143I GATC 5 cut(s) 166, 237, 289, 479, 718
Bsp1720I GCTNAGC 2 cut(s) 39, 462
BspACI CCGC 5 cut(s) 50, 97, 148, 202, 209
BspANI GGCC 2 cut(s) 59, 384
BspCNI CTCAG 1 cut(s) 454
BspEI TCCGGA 1 cut(s) 476
BspFNI CGCG 2 cut(s) 50, 171
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 4 cut(s) 271, 395, 420, 990
BspPI GGATC 4 cut(s) 174, 245, 297, 487
BspT104I TTCGAA 1 cut(s) 402
BspT107I GGYRCC 2 cut(s) 269, 418
BspTI CTTAAG 1 cut(s) 734
BsrBI CCGCTC 1 cut(s) 211
BsrDI GCAATG 2 cut(s) 786, 1053
BsrFI RCCGGY 2 cut(s) 412, 1008
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 4 cut(s) 542, 876, 964, 973
BssAI RCCGGY 2 cut(s) 412, 1008
BssECI CCNNGG 3 cut(s) 229, 285, 851
BssMI GATC 5 cut(s) 166, 237, 289, 479, 718
BssNI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
BssT1I CCWWGG 1 cut(s) 851
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 1056
BstACI GRCGYC 5 cut(s) 18, 135, 243, 270, 419
BstAFI CTTAAG 1 cut(s) 734
BstAUI TGTACA 1 cut(s) 948
BstBI TTCGAA 1 cut(s) 402
BstC8I GCNNGC 4 cut(s) 120, 152, 459, 917
BstDEI CTNAG 7 cut(s) 39, 330, 462, 752, 781, 911, 1073
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 804
BstFNI CGCG 2 cut(s) 50, 171
BstH2I RGCGCY 2 cut(s) 273, 422
BstHHI GCGC 4 cut(s) 50, 173, 272, 421
BstKTI GATC 5 cut(s) 169, 240, 292, 482, 721
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 5 cut(s) 166, 237, 289, 479, 718
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 6 cut(s) 56, 326, 418, 427, 463, 628
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstUI CGCG 2 cut(s) 50, 171
BstV1I GCAGC 1 cut(s) 1056
BstX2I RGATCY 1 cut(s) 718
BstXI CCANNNNNNTGG 1 cut(s) 659
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 384
BtgI CCRYGG 1 cut(s) 229
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 1 cut(s) 804
BtsIMutI CAGTG 1 cut(s) 883
Cac8I GCNNGC 4 cut(s) 120, 152, 459, 917
CaiI CAGNNNCTG 1 cut(s) 548
CciI TCATGA 1 cut(s) 940
CfoI GCGC 4 cut(s) 50, 173, 272, 421
Cfr10I RCCGGY 2 cut(s) 412, 1008
CseI GACGC 2 cut(s) 26, 143
Csp6I GTAC 4 cut(s) 265, 730, 949, 1057
CviAII CATG 3 cut(s) 343, 608, 941
CviQI GTAC 4 cut(s) 265, 730, 949, 1057
DdeI CTNAG 7 cut(s) 39, 330, 462, 752, 781, 911, 1073
DinI GGCGCC 2 cut(s) 271, 420
DpnI GATC 5 cut(s) 168, 239, 291, 481, 720
DpnII GATC 5 cut(s) 166, 237, 289, 479, 718
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 2 cut(s) 57, 382
EagI CGGCCG 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 851
Eco24I GRGCYC 2 cut(s) 8, 503
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 851
EcoT38I GRGCYC 2 cut(s) 8, 503
EgeI GGCGCC 2 cut(s) 271, 420
EheI GGCGCC 2 cut(s) 271, 420
ErhI CCWWGG 1 cut(s) 851
FaeI CATG 3 cut(s) 346, 611, 944
FatI CATG 3 cut(s) 342, 607, 940
Fnu4HI GCNGC 3 cut(s) 51, 97, 1070
FokI GGATG 1 cut(s) 811
FriOI GRGCYC 2 cut(s) 8, 503
Fsp4HI GCNGC 3 cut(s) 51, 97, 1070
FspBI CTAG 2 cut(s) 311, 623
GlaI GCGC 4 cut(s) 49, 172, 271, 420
GluI GCNGC 3 cut(s) 51, 97, 1070
HaeII RGCGCY 2 cut(s) 273, 422
HaeIII GGCC 2 cut(s) 59, 384
HapII CCGG 4 cut(s) 413, 477, 604, 1009
HgaI GACGC 2 cut(s) 26, 143
HhaI GCGC 4 cut(s) 50, 173, 272, 421
Hin1I GRCGYC 5 cut(s) 18, 135, 243, 270, 419
Hin1II CATG 3 cut(s) 346, 611, 944
Hin6I GCGC 4 cut(s) 48, 171, 270, 419
HinP1I GCGC 4 cut(s) 48, 171, 270, 419
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 2 cut(s) 152, 629
HinfI GANTC 3 cut(s) 26, 592, 1096
HpaII CCGG 4 cut(s) 413, 477, 604, 1009
HphI GGTGA 3 cut(s) 116, 521, 590
Hpy166II GTNNAC 4 cut(s) 181, 280, 749, 949
Hpy188I TCNGA 3 cut(s) 16, 25, 1095
Hpy188III TCNNGA 8 cut(s) 33, 158, 477, 493, 596, 839, 941, 992
Hpy8I GTNNAC 4 cut(s) 181, 280, 749, 949
Hpy99I CGWCG 8 cut(s) 20, 34, 58, 137, 143, 221, 245, 248
HpyAV CCTTC 5 cut(s) 70, 76, 317, 490, 1018
HpyCH4III ACNGT 1 cut(s) 1056
HpyCH4IV ACGT 2 cut(s) 216, 243
HpyCH4V TGCA 4 cut(s) 791, 881, 1046, 1079
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 326, 418, 427, 463, 628
HpyF3I CTNAG 7 cut(s) 39, 330, 462, 752, 781, 911, 1073
HpySE526I ACGT 2 cut(s) 216, 243
Hsp92I GRCGYC 5 cut(s) 18, 135, 243, 270, 419
Hsp92II CATG 3 cut(s) 346, 611, 944
HspAI GCGC 4 cut(s) 48, 171, 270, 419
KasI GGCGCC 2 cut(s) 269, 418
Kpn2I TCCGGA 1 cut(s) 476
Kzo9I GATC 5 cut(s) 166, 237, 289, 479, 718
LmnI GCTCC 7 cut(s) 11, 35, 216, 326, 393, 662, 994
Lsp1109I GCAGC 1 cut(s) 1056
LweI GCATC 1 cut(s) 307
MaeI CTAG 2 cut(s) 311, 623
MaeII ACGT 2 cut(s) 216, 243
MaeIII GTNAC 4 cut(s) 484, 667, 839, 974
MalI GATC 5 cut(s) 168, 239, 291, 481, 720
MbiI CCGCTC 1 cut(s) 211
MboI GATC 5 cut(s) 166, 237, 289, 479, 718
MboII GAAGA 5 cut(s) 310, 554, 688, 713, 800
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 2 cut(s) 8, 503
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 7 cut(s) 450, 616, 653, 693, 831, 871, 934
MluNI TGGCCA 1 cut(s) 384
Mly113I GGCGCC 2 cut(s) 270, 419
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 4 cut(s) 39, 48, 275, 877
Mox20I TGGCCA 1 cut(s) 384
MroI TCCGGA 1 cut(s) 476
MscI TGGCCA 1 cut(s) 384
MseI TTAA 5 cut(s) 518, 692, 707, 735, 1002
MslI CAYNNNNRTG 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 767
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 4 cut(s) 413, 477, 604, 1009
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 2 cut(s) 50, 171
MwoI GCNNNNNNNGC 6 cut(s) 56, 326, 418, 427, 463, 628
NarI GGCGCC 2 cut(s) 270, 419
NdeII GATC 5 cut(s) 166, 237, 289, 479, 718
NlaIII CATG 3 cut(s) 346, 611, 944
NlaIV GGNNCC 4 cut(s) 271, 395, 420, 990
NmuCI GTSAC 1 cut(s) 839
NspV TTCGAA 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 158
PagI TCATGA 1 cut(s) 940
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 592, 1096
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 52, 98, 1071
PleI GAGTC 1 cut(s) 20
PluTI GGCGCC 2 cut(s) 273, 422
PpsI GAGTC 1 cut(s) 20
PsiI TTATAA 1 cut(s) 830
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 4 cut(s) 271, 395, 420, 990
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 1 cut(s) 718
PsyI GACNNNGTC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 4 cut(s) 266, 731, 950, 1058
RsaNI GTAC 4 cut(s) 265, 730, 949, 1057
RseI CAYNNNNRTG 1 cut(s) 531
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 5 cut(s) 518, 692, 707, 735, 1002
SatI GCNGC 3 cut(s) 51, 97, 1070
Sau3AI GATC 5 cut(s) 166, 237, 289, 479, 718
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 2 cut(s) 8, 503
SfaNI GCATC 1 cut(s) 307
SfoI GGCGCC 2 cut(s) 271, 420
Sfr274I CTCGAG 1 cut(s) 158
SfuI TTCGAA 1 cut(s) 402
SgrAI CRCCGGYG 1 cut(s) 1008
SlaI CTCGAG 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 158, 734
SmoI CTYRAG 2 cut(s) 158, 734
Sse9I AATT 7 cut(s) 450, 616, 653, 693, 831, 871, 934
SsiI CCGC 5 cut(s) 50, 97, 148, 202, 209
SspDI GGCGCC 2 cut(s) 269, 418
SspMI CTAG 2 cut(s) 311, 623
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 1 cut(s) 851
TaaI ACNGT 1 cut(s) 1056
TaiI ACGT 2 cut(s) 219, 246
TaqI TCGA 4 cut(s) 159, 240, 261, 402
TasI AATT 7 cut(s) 450, 616, 653, 693, 831, 871, 934
TatI WGTACW 1 cut(s) 948
TauI GCSGC 2 cut(s) 53, 99
TfiI GAWTC 2 cut(s) 592, 1096
Tru1I TTAA 5 cut(s) 518, 692, 707, 735, 1002
Tru9I TTAA 5 cut(s) 518, 692, 707, 735, 1002
TscAI CASTG 1 cut(s) 883
TseFI GTSAC 1 cut(s) 839
TseI GCWGC 1 cut(s) 1069
Tsp45I GTSAC 1 cut(s) 839
TspDTI ATGAA 3 cut(s) 624, 713, 824
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 1 cut(s) 883
Tth111I GACNNNGTC 1 cut(s) 137
Vha464I CTTAAG 1 cut(s) 734
XapI RAATTY 2 cut(s) 616, 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XhoI CTCGAG 1 cut(s) 158
XspI CTAG 2 cut(s) 311, 623
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.