Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008776

Physical Location & Seq

Genomic Coordinates Forward (+)

4756696 .. 4757750

Gene Structure 1055 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1067600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 324 bp

ATGGCTCCTGAAGTTTTTAGGCACCGGAGATATGATAAGAAGGTTGACGTGTTCTCTTTTGCAATGATACTATACGAGATGCTTGAAGGGGACCCACCACTTTCGAATTACGAGCCTTATGAAGCAGCCAAATATGTGGCGGAAGGACACAGGCCTTTGTTTCATGGAAAAGGTCACACCCCGGAACTGAAAGAGTTAATAGAGCAGTGTTGGTCTGCCGACATGAACCAGAGGCCTTCTTTCTTGGACATTCTCAAAAGGCTTGAGAAGGTAAAGGAAAAAGTTCTACAAGCAGACCATCACTGGAACATATTTAATGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004712 GO:0004713 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009653 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019538 GO:0023052 GO:0030154 GO:0030435 GO:0032502 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Pkinase_Tyr

Protein Analysis

108

Amino Acids

12.69

Weight (kDa)

6.29

Isoelectric Point (pI)

41.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	1 - 88	7.5e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	1 - 86	2.3e-13	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0008776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14000
fragaria_vesca	FvH4_4g30780 FvH4_4g30780 FvH4_4g30780
malus_domestica	MD13G1068700.v1.1 MD16G1067500.v1.1 MD16G1067600.v1.1
prunus_persica	Prupe.1G279200_v2.0.a1
pyrus_communis	pycom13g06050 pycom16g05900
rosa_chinensis	RchiOBHm_Chr4g0439171
rosa_laevigata	RLG00000006291
rosa_multiflora	Rmu_sc0002236.1_g000008 Rmu_sc0027506.1_g000011
rosa_roxburghii	Rroxscaffold_5G00380150 Rroxscaffold_5G00380160
rosa_rugosa	Rorug04G0316400
rosa_samantha	Rh4AG367200 Rh4BG378800 Rh4CG393200 Rh4DG373400
rosa_wichuraiana	Rw0G011680 Rw5G028500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	21
AciI	CCGC	1 cut(s)	140
AcuI	CTGAAG	1 cut(s)	30
AflIII	ACRYGT	1 cut(s)	48
AgsI	TTSAA	1 cut(s)	86
AjiI	CACGTC	1 cut(s)	49
AoxI	GGCC	2 cut(s)	152, 233
ApeKI	GCWGC	1 cut(s)	125
Asp700I	GAANNNNTTC	1 cut(s)	282
AspS9I	GGNCC	1 cut(s)	91
AsuC2I	CCSGG	1 cut(s)	182
AsuII	TTCGAA	1 cut(s)	104
AvaII	GGWCC	1 cut(s)	91
BanI	GGYRCC	1 cut(s)	21
BbvI	GCAGC	1 cut(s)	137
BccI	CCATC	1 cut(s)	306
BcnI	CCSGG	1 cut(s)	182
BisI	GCNGC	1 cut(s)	126
BlsI	GCNGC	1 cut(s)	127
Bme1390I	CCNGG	1 cut(s)	182
Bme18I	GGWCC	1 cut(s)	91
BmgBI	CACGTC	1 cut(s)	49
BmgT120I	GGNCC	1 cut(s)	91
BmiI	GGNNCC	4 cut(s)	6, 23, 92, 93
BmrFI	CCNGG	1 cut(s)	182
BmsI	GCATC	1 cut(s)	69
Bpu14I	TTCGAA	1 cut(s)	104
BpuEI	CTTGAG	1 cut(s)	284
BpuMI	CCSGG	1 cut(s)	182
BsaJI	CCNNGG	1 cut(s)	180
BsaWI	WCCGGW	1 cut(s)	24
Bse1I	ACTGG	1 cut(s)	308
Bse3DI	GCAATG	1 cut(s)	69
BseDI	CCNNGG	1 cut(s)	180
BseMI	GCAATG	1 cut(s)	69
BseNI	ACTGG	1 cut(s)	308
BseXI	GCAGC	1 cut(s)	137
BshFI	GGCC	2 cut(s)	154, 235
BshNI	GGYRCC	1 cut(s)	21
BsiSI	CCGG	2 cut(s)	25, 182
BslFI	GGGAC	1 cut(s)	104
BsmFI	GGGAC	1 cut(s)	104
BsnI	GGCC	2 cut(s)	154, 235
Bsp119I	TTCGAA	1 cut(s)	104
BspACI	CCGC	1 cut(s)	140
BspANI	GGCC	2 cut(s)	154, 235
BspLI	GGNNCC	4 cut(s)	6, 23, 92, 93
BspT104I	TTCGAA	1 cut(s)	104
BspT107I	GGYRCC	1 cut(s)	21
BsrDI	GCAATG	1 cut(s)	69
BsrI	ACTGG	1 cut(s)	308
BssECI	CCNNGG	1 cut(s)	180
BstBI	TTCGAA	1 cut(s)	104
BstSCI	CCNGG	1 cut(s)	180
BstV1I	GCAGC	1 cut(s)	137
BstXI	CCANNNNNNTGG	1 cut(s)	136
BsuRI	GGCC	2 cut(s)	154, 235
BtrI	CACGTC	1 cut(s)	49
BtsI	GCAGTG	1 cut(s)	212
BtsIMutI	CAGTG	2 cut(s)	212, 301
Cfr13I	GGNCC	1 cut(s)	91
CviAII	CATG	2 cut(s)	164, 223
CviJI	RGCY	6 cut(s)	5, 115, 128, 154, 235, 262
CviKI_1	RGCY	6 cut(s)	5, 115, 128, 154, 235, 262
EciI	GGCGGA	1 cut(s)	155
Eco147I	AGGCCT	2 cut(s)	154, 235
Eco47I	GGWCC	1 cut(s)	91
Eco57I	CTGAAG	1 cut(s)	30
EcoO109I	RGGNCCY	1 cut(s)	91
FaeI	CATG	2 cut(s)	167, 226
FaiI	YATR	7 cut(s)	33, 73, 120, 135, 165, 224, 311
FaqI	GGGAC	1 cut(s)	104
FatI	CATG	2 cut(s)	163, 222
Fnu4HI	GCNGC	1 cut(s)	126
Fsp4HI	GCNGC	1 cut(s)	126
GluI	GCNGC	1 cut(s)	126
HaeIII	GGCC	2 cut(s)	154, 235
HapII	CCGG	2 cut(s)	25, 182
Hin1II	CATG	2 cut(s)	167, 226
HincII	GTYRAC	1 cut(s)	46
HindII	GTYRAC	1 cut(s)	46
HpaII	CCGG	2 cut(s)	25, 182
Hpy166II	GTNNAC	1 cut(s)	46
Hpy188III	TCNNGA	1 cut(s)	8
Hpy8I	GTNNAC	1 cut(s)	46
HpyAV	CCTTC	5 cut(s)	34, 80, 137, 246, 262
HpyCH4IV	ACGT	1 cut(s)	48
HpyCH4V	TGCA	1 cut(s)	62
HpySE526I	ACGT	1 cut(s)	48
Hsp92II	CATG	2 cut(s)	167, 226
KflI	GGGWCCC	1 cut(s)	91
LmnI	GCTCC	1 cut(s)	10
LpnPI	CCDG	6 cut(s)	21, 38, 136, 195, 242, 289
Lsp1109I	GCAGC	1 cut(s)	137
LweI	GCATC	1 cut(s)	69
MaeII	ACGT	1 cut(s)	48
MaeIII	GTNAC	1 cut(s)	173
MluCI	AATT	1 cut(s)	106
MnlI	CCTC	1 cut(s)	225
MroXI	GAANNNNTTC	1 cut(s)	282
MseI	TTAA	2 cut(s)	197, 315
MspI	CCGG	2 cut(s)	25, 182
MspR9I	CCNGG	1 cut(s)	182
NciI	CCSGG	1 cut(s)	182
NlaIII	CATG	2 cut(s)	167, 226
NlaIV	GGNNCC	4 cut(s)	6, 23, 92, 93
NmuCI	GTSAC	1 cut(s)	173
NspV	TTCGAA	1 cut(s)	104
PceI	AGGCCT	2 cut(s)	154, 235
PdmI	GAANNNNTTC	1 cut(s)	282
PkrI	GCNGC	1 cut(s)	127
PpuMI	RGGWCCY	1 cut(s)	91
Psp5II	RGGWCCY	1 cut(s)	91
PspN4I	GGNNCC	4 cut(s)	6, 23, 92, 93
PspPI	GGNCC	1 cut(s)	91
PspPPI	RGGWCCY	1 cut(s)	91
SaqAI	TTAA	2 cut(s)	197, 315
SatI	GCNGC	1 cut(s)	126
Sau96I	GGNCC	1 cut(s)	91
ScrFI	CCNGG	1 cut(s)	182
SetI	ASST	4 cut(s)	45, 51, 175, 273
SfaNI	GCATC	1 cut(s)	69
SfuI	TTCGAA	1 cut(s)	104
SinI	GGWCC	1 cut(s)	91
SmlI	CTYRAG	1 cut(s)	263
SmoI	CTYRAG	1 cut(s)	263
Sse9I	AATT	1 cut(s)	106
SseBI	AGGCCT	2 cut(s)	154, 235
SsiI	CCGC	1 cut(s)	140
StuI	AGGCCT	2 cut(s)	154, 235
StyD4I	CCNGG	1 cut(s)	180
TaiI	ACGT	1 cut(s)	51
TaqI	TCGA	1 cut(s)	104
TasI	AATT	1 cut(s)	106
Tru1I	TTAA	2 cut(s)	197, 315
Tru9I	TTAA	2 cut(s)	197, 315
TscAI	CASTG	2 cut(s)	212, 308
TseFI	GTSAC	1 cut(s)	173
TseI	GCWGC	1 cut(s)	125
Tsp45I	GTSAC	1 cut(s)	173
TspDTI	ATGAA	3 cut(s)	135, 152, 239
TspRI	CASTG	2 cut(s)	212, 308
VpaK11BI	GGWCC	1 cut(s)	91
XmnI	GAANNNNTTC	1 cut(s)	282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.