MD16G1085600.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014949
Physical Location & Seq
Reverse (-)
5990099 .. 5991264
1166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1085600.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGCATTCTATTCCCATTCCCTTTTGATCACCCTTCTAACCCTAGTATTACCCTTTCCCGCCGCGACAGCATCCCGTGCCCAGCTCACCGAAAACTACTACGATAAAACATGCCCAAAATTCAACGAAATCGTCCACCGCATCGTCACCGAAAAGCAAATAGCCACTCCCACTACCGCCGCTGCCGTCCTTCGCCTCTTCTTCCACGACTGCGTCCTCGAAGGCTGCGACGCCTCCCTCCTCATCGCTTCCAACTCCTTCAACAAGGCGGAGAGGGACGCCGACATCAACGTCGCCATCCCCGGGGATGGCTTCGACGTCGTCACCCGCATCAAGACCGCCCTCGAGCTCCAGTGCCCCGGGGTCGTCTCCTGCGCCGACATCCTCTCCGCCGCCGCACGCAATCTTGTCAACATGGTCGGTGGACCCCACTACACCCTCCGTTTCGGCCGCAAAGACGGTCTAATCTCCCGGGCCGATCGTGTGGAAGGCCACTACGCCAGGACCAACATGACCATCTCCGAGATCATCAACCTCTTCTCCTCCATCAATCTCTCCGTCCAGGACCTCGTCGCCCTAAGCGGGGCCCACACTATCGGTTTCTCCCACTGCAACGAGTTTGCCAAACGCCTCTTCAATTTCAGCACGACAGCGGAGACCGACCCCGCCCTCAATCGCAACTACGCGGAGGGTCTGAGGAAGCTCTGCGCCAATTACACTACCAACCCCGGGATGTCGGCGTTCAACGACGTCATGACCCCGGGGAAGTTCGACAACTTGTATTTCCAAAACTTGCAAAGGGGTCTAGGGTTACTGGCGACAGACAACGCCCTAGTGACGGATCCGCGGACGAAGCCGTTTGTGGATCTCTACGCCGCCGACCAGGCAAAGTTTTTCGAGGATTTCGGCCAGGCCATACAGAGGGTCAGCTTGATGAAAGTGAAGACAGGGAAGCATGGGGAGGTCCGACGCCGATGCGACGCCTTCAACAACCTTCAACAACCTCAAGACTCGAACAACAAACCCCAAGACTCGAACAACAAACCTCAAGACTTGAACAAGAAGAAAAAAAATTCCTCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

362

Amino Acids

39.87

Weight (kDa)

8.54

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 49 - 294 1.9e-71 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24110
malus_domestica MD13G1086200.v1.1 MD16G1085600.v1.1
prunus_persica Prupe.1G260500_v2.0.a1
pyrus_communis pycom13g07440
rosa_chinensis RchiOBHm_Chr4g0444711
rosa_laevigata RLG00000005810
rosa_multiflora Rmu_co8403781.1_g000001 Rmu_sc0012443.1_g000010
rosa_roxburghii Rroxscaffold_5G00385220
rosa_rugosa Rorug04G0354900
rosa_samantha Rh4AG415000 Rh4BG426500 Rh4CG441500 Rh4DG422500
rosa_wichuraiana Rw4G035640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 290
AatII GACGTC 2 cut(s) 321, 753
AccII CGCG 3 cut(s) 65, 686, 847
AclWI GGATC 3 cut(s) 836, 849, 873
AcoI YGGCCR 2 cut(s) 448, 907
AcsI RAATTY 2 cut(s) 119, 1072
AcyI GRCGYC 6 cut(s) 231, 279, 318, 750, 970, 981
AfiI CCNNNNNNNGG 7 cut(s) 303, 308, 582, 583, 729, 838, 921
AgsI TTSAA 7 cut(s) 124, 262, 637, 745, 988, 998, 1057
AjnI CCWGG 4 cut(s) 500, 561, 882, 909
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 5 cut(s) 85, 349, 703, 930, 1082
AluI AGCT 5 cut(s) 85, 349, 703, 930, 1082
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 373, 650
AlwI GGATC 3 cut(s) 836, 849, 873
Ama87I CYCGRG 6 cut(s) 302, 344, 359, 471, 728, 760
AoxI GGCC 6 cut(s) 448, 474, 490, 585, 907, 912
ApaI GGGCCC 1 cut(s) 589
ApeKI GCWGC 2 cut(s) 182, 225
ApoI RAATTY 2 cut(s) 119, 1072
AspLEI GCGC 2 cut(s) 377, 710
AspS9I GGNCC 7 cut(s) 425, 474, 504, 565, 585, 586, 964
AsuHPI GGTGA 4 cut(s) 22, 79, 139, 316
AvaI CYCGRG 6 cut(s) 302, 344, 359, 471, 728, 760
AvaII GGWCC 4 cut(s) 425, 504, 565, 964
BaeGI GKGCMC 3 cut(s) 82, 359, 589
BamHI GGATCC 1 cut(s) 841
BanII GRGCYC 2 cut(s) 351, 589
BbsI GAAGAC 1 cut(s) 950
Bbv12I GWGCWC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 1078
BbvI GCAGC 2 cut(s) 169, 212
BccI CCATC 4 cut(s) 302, 305, 524, 554
BceAI ACGGC 2 cut(s) 170, 841
BcgI CGANNNNNNTGC 2 cut(s) 957, 991
BciT130I CCWGG 4 cut(s) 502, 563, 884, 911
BclI TGATCA 1 cut(s) 27
BcoDI GTCTC 2 cut(s) 373, 650
BfaI CTAG 3 cut(s) 44, 806, 833
BglI GCCNNNNNGGC 1 cut(s) 884
BisI GCNGC 8 cut(s) 63, 180, 183, 226, 393, 396, 451, 876
BlsI GCNGC 8 cut(s) 64, 181, 184, 227, 394, 397, 452, 877
Bme18I GGWCC 4 cut(s) 425, 504, 565, 964
BmeT110I CYCGRG 6 cut(s) 302, 344, 359, 471, 728, 760
BmgT120I GGNCC 7 cut(s) 425, 474, 504, 565, 585, 586, 964
BmiI GGNNCC 4 cut(s) 427, 586, 587, 843
BmsI GCATC 4 cut(s) 80, 150, 339, 965
BpiI GAAGAC 1 cut(s) 950
BpmI CTGGAG 1 cut(s) 335
Bpu10I CCTNAGC 2 cut(s) 578, 1078
BpuEI CTTGAG 2 cut(s) 990, 1032
BsaHI GRCGYC 6 cut(s) 231, 279, 318, 750, 970, 981
BsaI GGTCTC 1 cut(s) 650
BsaXI ACNNNNNCTCC 4 cut(s) 371, 401, 524, 554
Bsc4I CCNNNNNNNGG 7 cut(s) 303, 308, 582, 583, 729, 838, 921
Bse1I ACTGG 2 cut(s) 352, 819
BseBI CCWGG 4 cut(s) 502, 563, 884, 911
BseGI GGATG 5 cut(s) 71, 297, 313, 381, 738
BseLI CCNNNNNNNGG 7 cut(s) 303, 308, 582, 583, 729, 838, 921
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 2 cut(s) 352, 819
BseRI GAGGAG 2 cut(s) 230, 532
BseSI GKGCMC 3 cut(s) 82, 359, 589
BseX3I CGGCCG 1 cut(s) 448
BseXI GCAGC 2 cut(s) 169, 212
BseYI CCCAGC 1 cut(s) 81
Bsh1236I CGCG 3 cut(s) 65, 686, 847
Bsh1285I CGRYCG 2 cut(s) 451, 481
BshFI GGCC 6 cut(s) 450, 476, 492, 587, 909, 914
BsiEI CGRYCG 2 cut(s) 451, 481
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 6 cut(s) 302, 344, 359, 471, 728, 760
BsiSI CCGG 5 cut(s) 303, 360, 472, 729, 761
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 7 cut(s) 303, 308, 582, 583, 729, 838, 921
BsmAI GTCTC 2 cut(s) 373, 650
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 1 cut(s) 290
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 6 cut(s) 450, 476, 492, 587, 909, 914
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 6 cut(s) 302, 344, 359, 471, 728, 760
Bsp120I GGGCCC 1 cut(s) 585
Bsp1286I GDGCHC 4 cut(s) 82, 351, 359, 589
Bsp143I GATC 5 cut(s) 27, 478, 525, 841, 865
BspANI GGCC 6 cut(s) 450, 476, 492, 587, 909, 914
BspCNI CTCAG 1 cut(s) 687
BspFNI CGCG 3 cut(s) 65, 686, 847
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 4 cut(s) 427, 586, 587, 843
BspPI GGATC 3 cut(s) 836, 849, 873
BspTNI GGTCTC 1 cut(s) 650
BsrI ACTGG 2 cut(s) 352, 819
BssMI GATC 5 cut(s) 27, 478, 525, 841, 865
BssNI GRCGYC 6 cut(s) 231, 279, 318, 750, 970, 981
Bst2UI CCWGG 4 cut(s) 502, 563, 884, 911
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 3 cut(s) 203, 542, 638
BstACI GRCGYC 6 cut(s) 231, 279, 318, 750, 970, 981
BstAPI GCANNNNNTGC 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 3 cut(s) 578, 695, 1078
BstDSI CCRYGG 1 cut(s) 845
BstF5I GGATG 5 cut(s) 71, 297, 313, 381, 738
BstFNI CGCG 3 cut(s) 65, 686, 847
BstHHI GCGC 2 cut(s) 377, 710
BstKTI GATC 5 cut(s) 30, 481, 528, 844, 868
BstMAI GTCTC 2 cut(s) 373, 650
BstMBI GATC 5 cut(s) 27, 478, 525, 841, 865
BstMCI CGRYCG 2 cut(s) 451, 481
BstMWI GCNNNNNNNGC 4 cut(s) 68, 77, 853, 884
BstNI CCWGG 4 cut(s) 502, 563, 884, 911
BstNSI RCATGY 1 cut(s) 114
BstSLI GKGCMC 3 cut(s) 82, 359, 589
BstUI CGCG 3 cut(s) 65, 686, 847
BstV1I GCAGC 2 cut(s) 169, 212
BstV2I GAAGAC 1 cut(s) 950
BstX2I RGATCY 2 cut(s) 841, 865
BstYI RGATCY 2 cut(s) 841, 865
BstZI CGGCCG 1 cut(s) 448
BsuRI GGCC 6 cut(s) 450, 476, 492, 587, 909, 914
BtgI CCRYGG 1 cut(s) 845
BtgZI GCGATG 1 cut(s) 229
BtsCI GGATG 5 cut(s) 71, 297, 313, 381, 738
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 2 cut(s) 359, 607
Cac8I GCNNGC 1 cut(s) 400
CciI TCATGA 1 cut(s) 753
CfoI GCGC 2 cut(s) 377, 710
Cfr13I GGNCC 7 cut(s) 425, 474, 504, 565, 585, 586, 964
Cfr42I CCGCGG 1 cut(s) 848
Cfr9I CCCGGG 5 cut(s) 302, 359, 471, 728, 760
CseI GACGC 5 cut(s) 202, 239, 287, 978, 989
CviAII CATG 5 cut(s) 111, 415, 511, 754, 956
DdeI CTNAG 3 cut(s) 578, 695, 1078
DpnI GATC 5 cut(s) 29, 480, 527, 843, 867
DpnII GATC 5 cut(s) 27, 478, 525, 841, 865
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 448, 907
EagI CGGCCG 1 cut(s) 448
Eam1104I CTCTTC 3 cut(s) 203, 542, 638
EarI CTCTTC 3 cut(s) 203, 542, 638
EciI GGCGGA 2 cut(s) 284, 379
Ecl136II GAGCTC 1 cut(s) 349
EclXI CGGCCG 1 cut(s) 448
Eco24I GRGCYC 2 cut(s) 351, 589
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 4 cut(s) 425, 504, 565, 964
Eco52I CGGCCG 1 cut(s) 448
Eco53kI GAGCTC 1 cut(s) 349
Eco88I CYCGRG 6 cut(s) 302, 344, 359, 471, 728, 760
EcoICRI GAGCTC 1 cut(s) 349
EcoO109I RGGNCCY 2 cut(s) 565, 585
EcoRII CCWGG 4 cut(s) 500, 561, 882, 909
EcoT38I GRGCYC 2 cut(s) 351, 589
Esp3I CGTCTC 1 cut(s) 373
FaeI CATG 5 cut(s) 114, 418, 514, 757, 959
FaiI YATR 6 cut(s) 112, 416, 512, 755, 917, 957
FaqI GGGAC 1 cut(s) 290
FatI CATG 5 cut(s) 110, 414, 510, 753, 955
FauI CCCGC 4 cut(s) 67, 335, 575, 673
FbaI TGATCA 1 cut(s) 27
Fnu4HI GCNGC 8 cut(s) 63, 180, 183, 226, 393, 396, 451, 876
FokI GGATG 5 cut(s) 58, 284, 320, 368, 745
FriOI GRGCYC 2 cut(s) 351, 589
Fsp4HI GCNGC 8 cut(s) 63, 180, 183, 226, 393, 396, 451, 876
FspBI CTAG 3 cut(s) 44, 806, 833
GlaI GCGC 2 cut(s) 376, 709
GluI GCNGC 8 cut(s) 63, 180, 183, 226, 393, 396, 451, 876
GsaI CCCAGC 1 cut(s) 85
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 6 cut(s) 450, 476, 492, 587, 909, 914
HapII CCGG 5 cut(s) 303, 360, 472, 729, 761
HgaI GACGC 5 cut(s) 202, 239, 287, 978, 989
HhaI GCGC 2 cut(s) 377, 710
Hin1I GRCGYC 6 cut(s) 231, 279, 318, 750, 970, 981
Hin1II CATG 5 cut(s) 114, 418, 514, 757, 959
Hin6I GCGC 2 cut(s) 375, 708
HinP1I GCGC 2 cut(s) 375, 708
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 2 cut(s) 1010, 1031
HpaII CCGG 5 cut(s) 303, 360, 472, 729, 761
HphI GGTGA 4 cut(s) 22, 79, 139, 316
Hpy166II GTNNAC 3 cut(s) 136, 412, 425
Hpy188I TCNGA 3 cut(s) 523, 696, 968
Hpy188III TCNNGA 4 cut(s) 334, 754, 1007, 1049
Hpy8I GTNNAC 3 cut(s) 136, 412, 425
Hpy99I CGWCG 8 cut(s) 233, 296, 320, 323, 575, 752, 972, 983
HpyAV CCTTC 7 cut(s) 44, 200, 215, 268, 482, 994, 1004
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 3 cut(s) 291, 318, 750
HpyCH4V TGCA 2 cut(s) 612, 796
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 77, 853, 884
HpyF3I CTNAG 3 cut(s) 578, 695, 1078
HpySE526I ACGT 3 cut(s) 291, 318, 750
Hsp92I GRCGYC 6 cut(s) 231, 279, 318, 750, 970, 981
Hsp92II CATG 5 cut(s) 114, 418, 514, 757, 959
HspAI GCGC 2 cut(s) 375, 708
Ksp22I TGATCA 1 cut(s) 27
KspI CCGCGG 1 cut(s) 848
Kzo9I GATC 5 cut(s) 27, 478, 525, 841, 865
LmnI GCTCC 1 cut(s) 354
Lsp1109I GCAGC 2 cut(s) 169, 212
LweI GCATC 4 cut(s) 80, 150, 339, 965
MaeI CTAG 3 cut(s) 44, 806, 833
MaeII ACGT 3 cut(s) 291, 318, 750
MaeIII GTNAC 4 cut(s) 145, 322, 810, 835
MalI GATC 5 cut(s) 29, 480, 527, 843, 867
MboI GATC 5 cut(s) 27, 478, 525, 841, 865
MboII GAAGA 6 cut(s) 190, 193, 529, 625, 955, 1075
MflI RGATCY 2 cut(s) 841, 865
MhlI GDGCHC 4 cut(s) 82, 351, 359, 589
MluCI AATT 4 cut(s) 119, 637, 712, 1072
MlyI GAGTC 2 cut(s) 1004, 1025
MmeI TCCRAC 2 cut(s) 276, 991
MseI TTAA 1 cut(s) 1084
MspA1I CMGCKG 3 cut(s) 182, 653, 847
MspI CCGG 5 cut(s) 303, 360, 472, 729, 761
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 4 cut(s) 502, 563, 884, 911
MvnI CGCG 3 cut(s) 65, 686, 847
MwoI GCNNNNNNNGC 4 cut(s) 68, 77, 853, 884
NdeII GATC 5 cut(s) 27, 478, 525, 841, 865
NlaIII CATG 5 cut(s) 114, 418, 514, 757, 959
NlaIV GGNNCC 4 cut(s) 427, 586, 587, 843
NmuCI GTSAC 3 cut(s) 145, 322, 835
NspI RCATGY 1 cut(s) 114
PaeR7I CTCGAG 1 cut(s) 344
PagI TCATGA 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 5
PflFI GACNNNGTC 3 cut(s) 212, 320, 569
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 8 cut(s) 64, 181, 184, 227, 394, 397, 452, 877
Ple19I CGATCG 1 cut(s) 481
PleI GAGTC 2 cut(s) 1004, 1025
PpsI GAGTC 2 cut(s) 1004, 1025
PpuMI RGGWCCY 1 cut(s) 565
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 565
Psp6I CCWGG 4 cut(s) 500, 561, 882, 909
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 4 cut(s) 500, 561, 882, 909
PspN4I GGNNCC 4 cut(s) 427, 586, 587, 843
PspOMI GGGCCC 1 cut(s) 585
PspPI GGNCC 7 cut(s) 425, 474, 504, 565, 585, 586, 964
PspPPI RGGWCCY 1 cut(s) 565
PspXI VCTCGAGB 1 cut(s) 344
PsuI RGATCY 2 cut(s) 841, 865
PsyI GACNNNGTC 3 cut(s) 212, 320, 569
PvuI CGATCG 1 cut(s) 481
SacI GAGCTC 1 cut(s) 351
SacII CCGCGG 1 cut(s) 848
SaqAI TTAA 1 cut(s) 1084
SatI GCNGC 8 cut(s) 63, 180, 183, 226, 393, 396, 451, 876
Sau3AI GATC 5 cut(s) 27, 478, 525, 841, 865
Sau96I GGNCC 7 cut(s) 425, 474, 504, 565, 585, 586, 964
SchI GAGTC 2 cut(s) 1004, 1025
SduI GDGCHC 4 cut(s) 82, 351, 359, 589
SfaNI GCATC 4 cut(s) 80, 150, 339, 965
Sfr274I CTCGAG 1 cut(s) 344
Sfr303I CCGCGG 1 cut(s) 848
SgrBI CCGCGG 1 cut(s) 848
SinI GGWCC 4 cut(s) 425, 504, 565, 964
SlaI CTCGAG 1 cut(s) 344
SmaI CCCGGG 5 cut(s) 304, 361, 473, 730, 762
SmlI CTYRAG 3 cut(s) 344, 1005, 1047
SmoI CTYRAG 3 cut(s) 344, 1005, 1047
Sse9I AATT 4 cut(s) 119, 637, 712, 1072
SspMI CTAG 3 cut(s) 44, 806, 833
SstI GAGCTC 1 cut(s) 351
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 3 cut(s) 294, 321, 753
TaqI TCGA 7 cut(s) 219, 315, 345, 771, 897, 1013, 1034
TaqII GACCGA 1 cut(s) 674
TasI AATT 4 cut(s) 119, 637, 712, 1072
TauI GCSGC 6 cut(s) 65, 182, 395, 398, 453, 878
Tru1I TTAA 1 cut(s) 1084
Tru9I TTAA 1 cut(s) 1084
TscAI CASTG 2 cut(s) 359, 614
TseFI GTSAC 3 cut(s) 145, 322, 835
TseI GCWGC 2 cut(s) 182, 225
Tsp45I GTSAC 3 cut(s) 145, 322, 835
TspDTI ATGAA 1 cut(s) 950
TspGWI ACGGA 3 cut(s) 431, 547, 854
TspMI CCCGGG 5 cut(s) 302, 359, 471, 728, 760
TspRI CASTG 2 cut(s) 359, 614
Tth111I GACNNNGTC 3 cut(s) 212, 320, 569
VpaK11BI GGWCC 4 cut(s) 425, 504, 565, 964
XapI RAATTY 2 cut(s) 119, 1072
XceI RCATGY 1 cut(s) 114
XhoI CTCGAG 1 cut(s) 344
XmaI CCCGGG 5 cut(s) 302, 359, 471, 728, 760
XspI CTAG 3 cut(s) 44, 806, 833
ZraI GACGTC 2 cut(s) 319, 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.