Rh4DG422500

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014949
Physical Location & Seq
Forward (+)
63006089 .. 63006880
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG422500.1

Sequence Viewer

Length: 792 bp
ATGGTCGAGGGCTGCGACGCCTCCATTCTCATCGCCTCCAACTCCTTCAACAAAGCCGAGCGCGACTCCGACATCAACCTCTCCCTCGCCGGCGACGGCTTCGACGTCATCACCCGTATCAAGACCAACCTCGAGCTCACGTGCCCCGGCATCGTTTCCTGCGCCGACATCCTCGTCGCCGCCACCCGCAACCTCATCAACATGGTCGGCGGGCCCTACTACCCCGTCCGCTTCGGCCGCAAAGACGGCTTCGTCTCAAGAGCCGAAGCCGTCGAAGGTAACCTCGCCAGGGCCAACATGTCCTTGTCCCAGATCATCAACCTCTTCGCCGGTCGGAGACTCAACGTCCAGGAAATGGTCGCCCTCGCTGGGTCCCACACCATCGGATTCTCCCACTGCAAGGAGTTCAGTAATAGGCTCTTCAAGTTCAGCCCTACCTCCGAGTACGACCCGTCGTACAACCCCCAGTTCGCCGAGGGGCTCAGGAAGCTCTGCGCCAACTACACCCAGGACCCGCAGATGTCGGCCTTCAACGACGTCATGACACCTGGCAAGTTCGACAACATGTACTTTAGAAACTTGCAGAGGGGGCTAGGGTTGTTGCCTTCCGACCAGGCCATGGCGGTGGACAGGAGGACGAGGCCGCTCGTCGACCTCTACGCGGCGAACCAGACCAGGTGGTTCGACGATTTTTCTCGCGCTATGGAGAAGGTTAGCCTTTATAATGTTAAGATGGGAAGGAAGGGAGAGGTCCGGCGGAGATGTGATGCCTTCAACAATCTCAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

263

Amino Acids

29.43

Weight (kDa)

8.89

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 2 - 223 1.6e-66 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24110
malus_domestica MD13G1086200.v1.1 MD16G1085600.v1.1
prunus_persica Prupe.1G260500_v2.0.a1
pyrus_communis pycom13g07440
rosa_chinensis RchiOBHm_Chr4g0444711
rosa_laevigata RLG00000005810
rosa_multiflora Rmu_co8403781.1_g000001 Rmu_sc0012443.1_g000010
rosa_roxburghii Rroxscaffold_5G00385220
rosa_rugosa Rorug04G0354900
rosa_samantha Rh4AG415000 Rh4BG426500 Rh4CG441500 Rh4DG422500
rosa_wichuraiana Rw4G035640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
AasI GACNNNNNNGTC 2 cut(s) 173, 251
AatII GACGTC 2 cut(s) 108, 540
AccB7I CCANNNNNTGG 2 cut(s) 355, 619
AccBSI CCGCTC 1 cut(s) 646
AccI GTMKAC 1 cut(s) 651
AccII CGCG 3 cut(s) 63, 662, 699
AcoI YGGCCR 1 cut(s) 235
AcvI CACGTG 1 cut(s) 141
AcyI GRCGYC 3 cut(s) 18, 105, 537
AfaI GTAC 3 cut(s) 446, 458, 569
AfiI CCNNNNNNNGG 6 cut(s) 289, 355, 369, 400, 619, 661
AflIII ACRYGT 2 cut(s) 297, 564
AgsI TTSAA 4 cut(s) 49, 424, 532, 775
AhdI GACNNNNNGTC 1 cut(s) 344
AjnI CCWGG 6 cut(s) 287, 348, 507, 547, 612, 674
AluBI AGCT 2 cut(s) 136, 490
AluI AGCT 2 cut(s) 136, 490
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 259, 331
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 6 cut(s) 212, 235, 291, 525, 615, 641
ApaI GGGCCC 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 12
AspLEI GCGC 4 cut(s) 63, 164, 497, 701
AspS9I GGNCC 6 cut(s) 212, 213, 291, 372, 511, 751
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 3 cut(s) 372, 511, 751
BaeGI GKGCMC 2 cut(s) 146, 216
BanII GRGCYC 3 cut(s) 138, 216, 483
BbrPI CACGTG 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 138
BccI CCATC 2 cut(s) 389, 727
BceAI ACGGC 3 cut(s) 112, 254, 262
BciT130I CCWGG 6 cut(s) 289, 350, 509, 549, 614, 676
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 259, 331
BfaI CTAG 1 cut(s) 593
BisI GCNGC 5 cut(s) 13, 180, 238, 644, 663
BlsI GCNGC 5 cut(s) 14, 181, 239, 645, 664
Bme1390I CCNGG 7 cut(s) 147, 289, 350, 509, 549, 614, 676
Bme18I GGWCC 3 cut(s) 372, 511, 751
BmeRI GACNNNNNGTC 1 cut(s) 344
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 6 cut(s) 212, 213, 291, 372, 511, 751
BmiI GGNNCC 4 cut(s) 214, 373, 374, 513
BmrFI CCNGG 7 cut(s) 147, 289, 350, 509, 549, 614, 676
BmrI ACTGGG 1 cut(s) 460
BmsI GCATC 2 cut(s) 159, 757
BmuI ACTGGG 1 cut(s) 460
BplI GAGNNNNNCTC 2 cut(s) 50, 82
Bpu10I CCTNAGC 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 241
BpuMI CCSGG 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 141
BsaHI GRCGYC 3 cut(s) 18, 105, 537
BsaJI CCNNGG 5 cut(s) 145, 288, 474, 507, 618
Bsc4I CCNNNNNNNGG 6 cut(s) 289, 355, 369, 400, 619, 661
Bse118I RCCGGY 2 cut(s) 89, 329
Bse1I ACTGG 1 cut(s) 466
BseBI CCWGG 6 cut(s) 289, 350, 509, 549, 614, 676
BseDI CCNNGG 5 cut(s) 145, 288, 474, 507, 618
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 6 cut(s) 289, 355, 369, 400, 619, 661
BseMII CTCAG 1 cut(s) 496
BseNI ACTGG 1 cut(s) 466
BseSI GKGCMC 2 cut(s) 146, 216
BseX3I CGGCCG 1 cut(s) 235
BseYI CCCAGC 1 cut(s) 368
Bsh1236I CGCG 3 cut(s) 63, 662, 699
Bsh1285I CGRYCG 2 cut(s) 238, 334
BshFI GGCC 6 cut(s) 214, 237, 293, 527, 617, 643
BsiEI CGRYCG 2 cut(s) 238, 334
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 4 cut(s) 90, 147, 330, 754
BslFI GGGAC 2 cut(s) 292, 358
BslI CCNNNNNNNGG 6 cut(s) 289, 355, 369, 400, 619, 661
BsmAI GTCTC 2 cut(s) 259, 331
BsmBI CGTCTC 1 cut(s) 259
BsmFI GGGAC 2 cut(s) 292, 358
BsnI GGCC 6 cut(s) 214, 237, 293, 527, 617, 643
BsoBI CYCGRG 1 cut(s) 131
Bsp120I GGGCCC 1 cut(s) 212
Bsp1286I GDGCHC 4 cut(s) 138, 146, 216, 483
Bsp143I GATC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 618
BspANI GGCC 6 cut(s) 214, 237, 293, 527, 617, 643
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 3 cut(s) 63, 662, 699
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 4 cut(s) 214, 373, 374, 513
BspQI GCTCTTC 1 cut(s) 425
BsrBI CCGCTC 1 cut(s) 646
BsrFI RCCGGY 2 cut(s) 89, 329
BsrI ACTGG 1 cut(s) 466
BssAI RCCGGY 2 cut(s) 89, 329
BssECI CCNNGG 5 cut(s) 145, 288, 474, 507, 618
BssMI GATC 1 cut(s) 312
BssNI GRCGYC 3 cut(s) 18, 105, 537
BssT1I CCWWGG 1 cut(s) 618
Bst2UI CCWGG 6 cut(s) 289, 350, 509, 549, 614, 676
Bst6I CTCTTC 2 cut(s) 329, 425
BstACI GRCGYC 3 cut(s) 18, 105, 537
BstBAI YACGTR 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 91, 212
BstDEI CTNAG 1 cut(s) 482
BstDSI CCRYGG 1 cut(s) 618
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 3 cut(s) 63, 662, 699
BstHHI GCGC 4 cut(s) 63, 164, 497, 701
BstKTI GATC 1 cut(s) 315
BstMAI GTCTC 2 cut(s) 259, 331
BstMBI GATC 1 cut(s) 312
BstMCI CGRYCG 2 cut(s) 238, 334
BstMWI GCNNNNNNNGC 4 cut(s) 237, 246, 487, 589
BstNI CCWGG 6 cut(s) 289, 350, 509, 549, 614, 676
BstNSI RCATGY 2 cut(s) 301, 568
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 7 cut(s) 145, 287, 348, 507, 547, 612, 674
BstSLI GKGCMC 2 cut(s) 146, 216
BstUI CGCG 3 cut(s) 63, 662, 699
BstXI CCANNNNNNTGG 1 cut(s) 625
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 6 cut(s) 214, 237, 293, 527, 617, 643
BtgI CCRYGG 1 cut(s) 618
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 91, 212
CciI TCATGA 1 cut(s) 540
CfoI GCGC 4 cut(s) 63, 164, 497, 701
Cfr10I RCCGGY 2 cut(s) 89, 329
Cfr13I GGNCC 6 cut(s) 212, 213, 291, 372, 511, 751
CseI GACGC 1 cut(s) 26
CsiI ACCWGGT 1 cut(s) 674
Csp6I GTAC 3 cut(s) 445, 457, 568
CviAII CATG 5 cut(s) 202, 298, 541, 565, 619
CviQI GTAC 3 cut(s) 445, 457, 568
DdeI CTNAG 1 cut(s) 482
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
DrdI GACNNNNNNGTC 2 cut(s) 173, 251
DriI GACNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 2 cut(s) 173, 251
EaeI YGGCCR 1 cut(s) 235
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 2 cut(s) 329, 425
Eam1105I GACNNNNNGTC 1 cut(s) 344
EarI CTCTTC 2 cut(s) 329, 425
EciI GGCGGA 1 cut(s) 772
Ecl136II GAGCTC 1 cut(s) 136
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 618
Eco24I GRGCYC 3 cut(s) 138, 216, 483
Eco47I GGWCC 3 cut(s) 372, 511, 751
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 136
Eco72I CACGTG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 3 cut(s) 213, 372, 511
EcoO65I GGTNACC 1 cut(s) 278
EcoRII CCWGG 6 cut(s) 287, 348, 507, 547, 612, 674
EcoT14I CCWWGG 1 cut(s) 618
EcoT38I GRGCYC 3 cut(s) 138, 216, 483
ErhI CCWWGG 1 cut(s) 618
Esp3I CGTCTC 1 cut(s) 259
FaeI CATG 5 cut(s) 205, 301, 544, 568, 622
FaiI YATR 8 cut(s) 203, 299, 542, 566, 620, 704, 723, 790
FaqI GGGAC 2 cut(s) 292, 358
FatI CATG 5 cut(s) 201, 297, 540, 564, 618
FauI CCCGC 3 cut(s) 194, 203, 522
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 5 cut(s) 13, 180, 238, 644, 663
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 3 cut(s) 138, 216, 483
Fsp4HI GCNGC 5 cut(s) 13, 180, 238, 644, 663
FspBI CTAG 1 cut(s) 593
GlaI GCGC 4 cut(s) 62, 163, 496, 700
GluI GCNGC 5 cut(s) 13, 180, 238, 644, 663
GsaI CCCAGC 1 cut(s) 372
HaeIII GGCC 6 cut(s) 214, 237, 293, 527, 617, 643
HapII CCGG 4 cut(s) 90, 147, 330, 754
HgaI GACGC 1 cut(s) 26
HhaI GCGC 4 cut(s) 63, 164, 497, 701
Hin1I GRCGYC 3 cut(s) 18, 105, 537
Hin1II CATG 5 cut(s) 205, 301, 544, 568, 622
Hin6I GCGC 4 cut(s) 61, 162, 495, 699
HinP1I GCGC 4 cut(s) 61, 162, 495, 699
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 3 cut(s) 65, 339, 387
HpaII CCGG 4 cut(s) 90, 147, 330, 754
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 628, 652
Hpy188I TCNGA 5 cut(s) 70, 336, 386, 442, 610
Hpy188III TCNNGA 4 cut(s) 121, 258, 484, 541
Hpy8I GTNNAC 2 cut(s) 628, 652
Hpy99I CGWCG 9 cut(s) 20, 98, 107, 179, 275, 457, 539, 653, 689
HpyAV CCTTC 8 cut(s) 55, 269, 538, 615, 703, 732, 736, 781
HpyCH4IV ACGT 4 cut(s) 105, 140, 345, 537
HpyCH4V TGCA 2 cut(s) 399, 583
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 246, 487, 589
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 4 cut(s) 105, 140, 345, 537
Hsp92I GRCGYC 3 cut(s) 18, 105, 537
Hsp92II CATG 5 cut(s) 205, 301, 544, 568, 622
HspAI GCGC 4 cut(s) 61, 162, 495, 699
KflI GGGWCCC 1 cut(s) 372
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Kzo9I GATC 1 cut(s) 312
LguI GCTCTTC 1 cut(s) 425
LweI GCATC 2 cut(s) 159, 757
MabI ACCWGGT 1 cut(s) 674
MaeI CTAG 1 cut(s) 593
MaeII ACGT 4 cut(s) 105, 140, 345, 537
MaeIII GTNAC 1 cut(s) 278
MalI GATC 1 cut(s) 314
MbiI CCGCTC 1 cut(s) 646
MboI GATC 1 cut(s) 312
MboII GAAGA 2 cut(s) 316, 412
MhlI GDGCHC 4 cut(s) 138, 146, 216, 483
MlyI GAGTC 2 cut(s) 59, 333
MmeI TCCRAC 4 cut(s) 63, 93, 314, 633
MreI CGCCGGCG 1 cut(s) 89
MroNI GCCGGC 1 cut(s) 89
MseI TTAA 1 cut(s) 729
MslI CAYNNNNRTG 2 cut(s) 200, 623
MspI CCGG 4 cut(s) 90, 147, 330, 754
MspR9I CCNGG 7 cut(s) 147, 289, 350, 509, 549, 614, 676
MvaI CCWGG 6 cut(s) 289, 350, 509, 549, 614, 676
MvnI CGCG 3 cut(s) 63, 662, 699
MwoI GCNNNNNNNGC 4 cut(s) 237, 246, 487, 589
NaeI GCCGGC 1 cut(s) 91
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 618
NdeII GATC 1 cut(s) 312
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 5 cut(s) 205, 301, 544, 568, 622
NlaIV GGNNCC 4 cut(s) 214, 373, 374, 513
NmeAIII GCCGAG 2 cut(s) 82, 499
NspI RCATGY 2 cut(s) 301, 568
PaeR7I CTCGAG 1 cut(s) 131
PagI TCATGA 1 cut(s) 540
PciI ACATGT 2 cut(s) 297, 564
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 2 cut(s) 12, 102
PdiI GCCGGC 1 cut(s) 91
PfeI GAWTC 1 cut(s) 387
PflMI CCANNNNNTGG 2 cut(s) 355, 619
PfoI TCCNGGA 1 cut(s) 348
PkrI GCNGC 5 cut(s) 14, 181, 239, 645, 664
PleI GAGTC 2 cut(s) 59, 333
PmaCI CACGTG 1 cut(s) 141
PmlI CACGTG 1 cut(s) 141
PpsI GAGTC 2 cut(s) 59, 333
Ppu21I YACGTR 1 cut(s) 141
PpuMI RGGWCCY 2 cut(s) 372, 511
PscI ACATGT 2 cut(s) 297, 564
PsiI TTATAA 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 2 cut(s) 372, 511
Psp6I CCWGG 6 cut(s) 287, 348, 507, 547, 612, 674
PspCI CACGTG 1 cut(s) 141
PspEI GGTNACC 1 cut(s) 278
PspFI CCCAGC 1 cut(s) 368
PspGI CCWGG 6 cut(s) 287, 348, 507, 547, 612, 674
PspN4I GGNNCC 4 cut(s) 214, 373, 374, 513
PspOMI GGGCCC 1 cut(s) 212
PspPI GGNCC 6 cut(s) 212, 213, 291, 372, 511, 751
PspPPI RGGWCCY 2 cut(s) 372, 511
PspXI VCTCGAGB 1 cut(s) 131
RsaI GTAC 3 cut(s) 446, 458, 569
RsaNI GTAC 3 cut(s) 445, 457, 568
RseI CAYNNNNRTG 2 cut(s) 200, 623
SacI GAGCTC 1 cut(s) 138
SalI GTCGAC 1 cut(s) 650
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 1 cut(s) 729
SatI GCNGC 5 cut(s) 13, 180, 238, 644, 663
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 6 cut(s) 212, 213, 291, 372, 511, 751
SchI GAGTC 2 cut(s) 59, 333
ScrFI CCNGG 7 cut(s) 147, 289, 350, 509, 549, 614, 676
SduI GDGCHC 4 cut(s) 138, 146, 216, 483
SexAI ACCWGGT 1 cut(s) 674
SfaNI GCATC 2 cut(s) 159, 757
Sfr274I CTCGAG 1 cut(s) 131
SgrAI CRCCGGYG 1 cut(s) 89
SinI GGWCC 3 cut(s) 372, 511, 751
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 200, 623
SmlI CTYRAG 2 cut(s) 131, 256
SmoI CTYRAG 2 cut(s) 131, 256
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 7 cut(s) 145, 287, 348, 507, 547, 612, 674
StyI CCWWGG 1 cut(s) 618
TaiI ACGT 4 cut(s) 108, 143, 348, 540
TaqI TCGA 7 cut(s) 6, 102, 132, 273, 558, 651, 684
TatI WGTACW 1 cut(s) 567
TauI GCSGC 4 cut(s) 182, 240, 646, 665
TfiI GAWTC 1 cut(s) 387
Tru1I TTAA 1 cut(s) 729
Tru9I TTAA 1 cut(s) 729
TscAI CASTG 1 cut(s) 401
TseI GCWGC 1 cut(s) 12
TspRI CASTG 1 cut(s) 401
Van91I CCANNNNNTGG 2 cut(s) 355, 619
VpaK11BI GGWCC 3 cut(s) 372, 511, 751
XceI RCATGY 2 cut(s) 301, 568
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 651
XspI CTAG 1 cut(s) 593
ZraI GACGTC 2 cut(s) 106, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.