MD16G1087500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012534
Physical Location & Seq
Reverse (-)
6038622 .. 6041047
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1087500.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGATAAGCACCGGCAGGTTTCCACCTTCTTGCACTTCCAAATCCCTTCTCGCCGCCGCCATCTCGTTCCCGCCACACAAATCCCAATTGGGCTTCGCCGTCTTTCCCCTCCCATCTACTTCTTCTTCCTCACTCCCCACAGCCATTTTCAAAACCAGCTTCTTCCTCCCTCCCATTCGGCAAACCCACCGCGCCCATCTCGGGTTTCTCGCCGTCCAAGCCAGGAGAAGTAGCGGGCCGGGAGTCTCCTCCCAGAAAAGGCAACGGAGAGGCGGCGTTGTAACGGACGACGACTCTGATGACGAGGAGGAGTTGGAATTGGACGGCTCTGATGACGTGTTCGGTGGTGAGGAGGAAGACGATGACGAAGACGCGTTTGTGCCGTTTGGGAAGATGAAGAAGTGGCTGGAGAGGAAGCCGCGGGGGTTCGGAGAAGGCAAAGAGTACGACATTTCGATTGAAGAGAAGCTGCTCGAAGAAATTGAGAAGAGTAAGAAAGCACAGGCCGAGAGTAATCTCGCCAAGCTCTCCGAAAAGCCGAATTCGAAGGGAGACCAAAGCAAGAACAGCAGCAAAAGTGTTGGTGCGGTTCCGGATGGAGTTAGAGTGCGGGTGATCAATCTTCCGAAGAAGAAGAACATTCACAGGGATTTGAGCTCGGCTTTCAAGCTGGTTCCGGGTTTGATTAGTATAAACCCCGCGGTTTCCGGAAACAAGAAGACTAAGGACCCCATTTGCAAGGGTTTTGCTTTTGTTCATTTCAAGTCCAAGATCGATGCAACTAGGTTTGTAGAGATGTTTTCTTCACAGAATGTTACATTTGGTAAAATTCAAAAGCAGATAAAATGTGAGCTGGTAGATACGCCACAGCCGAATTTGGATCAGACACAATCAACAAATAACCGTAGTGCTCTCCCACTAATTGTTTCTGGATTGGAAGGAGATTATAAAGCTGATATCGACTCGGATGATTCTTCCATGGACTCATTGGAGGAGACTACCCTTGATGAATATGAGGGCTCTGATGTTGAGATTGTTGGACCAGAACTGGAAGACGTCATGGAACACTCATCGGATAACCATACTGCTCGTGAACTAACTGTTTCTGAATTGGAAGATGAGAAAGCTGATTCTGACTTGGATGATTCTTCCCAGGAGACATGGGAGGAGACCATAGTTGATGAATACGACGGCTCTGATTTTGAACTCAATGAATCAGAACTGGAAGATGCCATAGAGAATTTGGAATCTATCAGTGGCTCAGACCTAGATGGTGGAGATAGAGATAGCATGAAATCAAGGACGGAATCCGAGTCTTTATCAGCAGATTCATCCTCCTCGAAGCAGCTACTTGTCAAAGCAAAAGAAGAGAAGCTTCCCAGACAAAAGCCAGCTATGAAAGGGAAAGACGAAAGCTCGCCTAAGAATAAGAAGAAGAAGAAGCTTAATGTGAAACAAAAAGCAGTGAAGGTTCCAAAGTTGACGGTCGGCGGATCAGCCAAGAGGTTGAAGGTGAAGGAGAAGGCTGTGCTTGGTGACGTGTTCGCAAAATATGGGTCCAAAGAAGCTGTTTTGGCTTCAAAGGTAGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

532

Amino Acids

58.25

Weight (kDa)

5.11

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012534)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70200
fragaria_vesca FvH4_4g34980 FvH4_5g29371
malus_domestica MD16G1087500.v1.1
prunus_persica Prupe.1G259500_v2.0.a1
pyrus_communis pycom13g07510 pycom16g07510
rosa_chinensis RchiOBHm_Chr4g0444501
rosa_laevigata RLG00000005829
rosa_multiflora Rmu_co8304031.1_g000001 Rmu_sc0003839.1_g000008 Rmu_sc0035747.1_g000002
rosa_roxburghii Rroxscaffold_5G00384980
rosa_rugosa Rorug04G0353200
rosa_samantha Rh4AG413400 Rh4BG424900 Rh4CG439500 Rh4DG420400
rosa_wichuraiana Rw4G035510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
AatII GACGTC 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 6
AccII CGCG 4 cut(s) 192, 374, 421, 701
AccIII TCCGGA 2 cut(s) 592, 707
AclWI GGATC 2 cut(s) 888, 1501
AcsI RAATTY 4 cut(s) 541, 828, 874, 1240
AcyI GRCGYC 1 cut(s) 1056
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 2 cut(s) 201, 258
AflIII ACRYGT 3 cut(s) 336, 372, 1539
AgsI TTSAA 8 cut(s) 151, 461, 667, 763, 833, 1205, 1510, 1581
AjiI CACGTC 2 cut(s) 337, 1540
AjnI CCWGG 2 cut(s) 221, 1152
Alw21I GWGCWC 2 cut(s) 659, 913
Alw26I GTCTC 5 cut(s) 250, 546, 989, 1151, 1163
AlwI GGATC 2 cut(s) 888, 1501
Ama87I CYCGRG 1 cut(s) 200
Aor13HI TCCGGA 2 cut(s) 592, 707
AoxI GGCC 2 cut(s) 236, 504
ApeKI GCWGC 3 cut(s) 469, 570, 1345
ApoI RAATTY 4 cut(s) 541, 828, 874, 1240
ArsI GACNNNNNNTTYG 2 cut(s) 1541, 1573
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 4 cut(s) 236, 727, 1040, 1557
AsuC2I CCSGG 2 cut(s) 240, 678
AsuHPI GGTGA 4 cut(s) 359, 625, 1525, 1547
AsuII TTCGAA 1 cut(s) 545
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 3 cut(s) 727, 1040, 1557
BanII GRGCYC 2 cut(s) 659, 1022
BauI CACGAG 1 cut(s) 1089
BbsI GAAGAC 4 cut(s) 363, 375, 725, 1059
Bbv12I GWGCWC 2 cut(s) 659, 913
BbvI GCAGC 3 cut(s) 456, 582, 1357
BccI CCATC 5 cut(s) 68, 121, 204, 590, 1265
BceAI ACGGC 5 cut(s) 83, 197, 340, 367, 1207
BciT130I CCWGG 2 cut(s) 223, 1154
BclI TGATCA 1 cut(s) 615
BcnI CCSGG 2 cut(s) 240, 678
BcoDI GTCTC 5 cut(s) 250, 546, 989, 1151, 1163
BfaI CTAG 2 cut(s) 783, 1268
BfuAI ACCTGC 1 cut(s) 6
BisI GCNGC 7 cut(s) 54, 57, 274, 419, 470, 571, 1346
BlsI GCNGC 7 cut(s) 55, 58, 275, 420, 471, 572, 1347
Bme1390I CCNGG 4 cut(s) 223, 240, 678, 1154
Bme18I GGWCC 3 cut(s) 727, 1040, 1557
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 2 cut(s) 337, 1540
BmgT120I GGNCC 4 cut(s) 236, 727, 1040, 1557
BmiI GGNNCC 5 cut(s) 591, 675, 729, 1473, 1558
BmrFI CCNGG 4 cut(s) 223, 240, 678, 1154
BmsI GCATC 2 cut(s) 766, 1219
BpiI GAAGAC 4 cut(s) 363, 375, 725, 1059
BpmI CTGGAG 1 cut(s) 428
Bpu14I TTCGAA 1 cut(s) 545
BpuMI CCSGG 2 cut(s) 240, 678
Bsa29I ATCGAT 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 1056
BsaI GGTCTC 2 cut(s) 546, 1163
BsaJI CCNNGG 4 cut(s) 419, 699, 978, 1152
BsaWI WCCGGW 2 cut(s) 592, 707
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 258
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 1053, 1227
BseAI TCCGGA 2 cut(s) 592, 707
BseBI CCWGG 2 cut(s) 223, 1154
BseCI ATCGAT 1 cut(s) 774
BseDI CCNNGG 4 cut(s) 419, 699, 978, 1152
BseGI GGATG 4 cut(s) 601, 973, 1147, 1331
BseLI CCNNNNNNNGG 2 cut(s) 201, 258
BseMII CTCAG 1 cut(s) 1275
BseNI ACTGG 2 cut(s) 1053, 1227
BseRI GAGGAG 7 cut(s) 238, 320, 323, 365, 1007, 1181, 1327
BseXI GCAGC 3 cut(s) 456, 582, 1357
Bsh1236I CGCG 4 cut(s) 192, 374, 421, 701
Bsh1285I CGRYCG 1 cut(s) 1488
BshFI GGCC 2 cut(s) 238, 506
BshVI ATCGAT 1 cut(s) 774
BsiEI CGRYCG 1 cut(s) 1488
BsiHKAI GWGCWC 2 cut(s) 659, 913
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 5 cut(s) 12, 239, 593, 677, 708
BslI CCNNNNNNNGG 2 cut(s) 201, 258
BsmAI GTCTC 5 cut(s) 250, 546, 989, 1151, 1163
BsnI GGCC 2 cut(s) 238, 506
Bso31I GGTCTC 2 cut(s) 546, 1163
BsoBI CYCGRG 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 3 cut(s) 659, 913, 1022
Bsp13I TCCGGA 2 cut(s) 592, 707
Bsp143I GATC 4 cut(s) 615, 771, 880, 1493
Bsp19I CCATGG 1 cut(s) 978
BspANI GGCC 2 cut(s) 238, 506
BspCNI CTCAG 1 cut(s) 1274
BspDI ATCGAT 1 cut(s) 774
BspEI TCCGGA 2 cut(s) 592, 707
BspFNI CGCG 4 cut(s) 192, 374, 421, 701
BspLI GGNNCC 5 cut(s) 591, 675, 729, 1473, 1558
BspMI ACCTGC 1 cut(s) 6
BspPI GGATC 2 cut(s) 888, 1501
BspT104I TTCGAA 1 cut(s) 545
BspTNI GGTCTC 2 cut(s) 546, 1163
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 2 cut(s) 1053, 1227
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 4 cut(s) 419, 699, 978, 1152
BssMI GATC 4 cut(s) 615, 771, 880, 1493
BssNI GRCGYC 1 cut(s) 1056
BssSI CACGAG 1 cut(s) 1089
BssT1I CCWWGG 1 cut(s) 978
Bst2BI CACGAG 1 cut(s) 1089
Bst2UI CCWGG 2 cut(s) 223, 1154
Bst4CI ACNGT 3 cut(s) 905, 1102, 1486
Bst6I CTCTTC 3 cut(s) 456, 482, 1362
BstACI GRCGYC 1 cut(s) 1056
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 3 cut(s) 236, 1392, 1418
BstDEI CTNAG 3 cut(s) 723, 1261, 1422
BstDSI CCRYGG 3 cut(s) 419, 699, 978
BstF5I GGATG 4 cut(s) 601, 973, 1147, 1331
BstFNI CGCG 4 cut(s) 192, 374, 421, 701
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 4 cut(s) 618, 774, 883, 1496
BstMAI GTCTC 5 cut(s) 250, 546, 989, 1151, 1163
BstMBI GATC 4 cut(s) 615, 771, 880, 1493
BstMCI CGRYCG 1 cut(s) 1488
BstMWI GCNNNNNNNGC 3 cut(s) 218, 567, 1574
BstNI CCWGG 2 cut(s) 223, 1154
BstSCI CCNGG 4 cut(s) 221, 238, 676, 1152
BstUI CGCG 4 cut(s) 192, 374, 421, 701
BstV1I GCAGC 3 cut(s) 456, 582, 1357
BstV2I GAAGAC 4 cut(s) 363, 375, 725, 1059
Bsu15I ATCGAT 1 cut(s) 774
BsuRI GGCC 2 cut(s) 238, 506
BsuTUI ATCGAT 1 cut(s) 774
BtgI CCRYGG 3 cut(s) 419, 699, 978
BtrI CACGTC 2 cut(s) 337, 1540
BtsCI GGATG 4 cut(s) 601, 973, 1147, 1331
BtsI GCAGTG 1 cut(s) 1470
BtsIMutI CAGTG 2 cut(s) 1261, 1470
BveI ACCTGC 1 cut(s) 6
Cac8I GCNNGC 3 cut(s) 236, 1392, 1418
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 4 cut(s) 236, 727, 1040, 1557
Cfr42I CCGCGG 2 cut(s) 422, 702
ClaI ATCGAT 1 cut(s) 774
CseI GACGC 1 cut(s) 380
Csp6I GTAC 1 cut(s) 445
CviAII CATG 4 cut(s) 979, 1060, 1161, 1291
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 3 cut(s) 723, 1261, 1422
DpnI GATC 4 cut(s) 617, 773, 882, 1495
DpnII GATC 4 cut(s) 615, 771, 880, 1493
Eam1104I CTCTTC 3 cut(s) 456, 482, 1362
EarI CTCTTC 3 cut(s) 456, 482, 1362
EciI GGCGGA 1 cut(s) 1506
Ecl136II GAGCTC 1 cut(s) 657
Eco130I CCWWGG 1 cut(s) 978
Eco24I GRGCYC 2 cut(s) 659, 1022
Eco31I GGTCTC 2 cut(s) 546, 1163
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 3 cut(s) 727, 1040, 1557
Eco53kI GAGCTC 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 221, 1152
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 978
EcoT38I GRGCYC 2 cut(s) 659, 1022
ErhI CCWWGG 1 cut(s) 978
FaeI CATG 4 cut(s) 982, 1063, 1164, 1294
FalI AAGNNNNNCTT 2 cut(s) 1359, 1391
FatI CATG 4 cut(s) 978, 1059, 1160, 1290
FauI CCCGC 5 cut(s) 78, 227, 414, 603, 706
FbaI TGATCA 1 cut(s) 615
Fnu4HI GCNGC 7 cut(s) 54, 57, 274, 419, 470, 571, 1346
FokI GGATG 4 cut(s) 608, 980, 1154, 1318
FriOI GRGCYC 2 cut(s) 659, 1022
Fsp4HI GCNGC 7 cut(s) 54, 57, 274, 419, 470, 571, 1346
FspBI CTAG 2 cut(s) 783, 1268
GlaI GCGC 1 cut(s) 193
GluI GCNGC 7 cut(s) 54, 57, 274, 419, 470, 571, 1346
GsuI CTGGAG 1 cut(s) 428
HaeIII GGCC 2 cut(s) 238, 506
HapII CCGG 5 cut(s) 12, 239, 593, 677, 708
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 1056
Hin1II CATG 4 cut(s) 982, 1063, 1164, 1294
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 1 cut(s) 1482
HindII GTYRAC 1 cut(s) 1482
HindIII AAGCTT 2 cut(s) 1373, 1442
HpaII CCGG 5 cut(s) 12, 239, 593, 677, 708
HphI GGTGA 4 cut(s) 359, 625, 1525, 1547
Hpy166II GTNNAC 2 cut(s) 1094, 1482
Hpy188III TCNNGA 4 cut(s) 593, 708, 930, 1091
Hpy8I GTNNAC 2 cut(s) 1094, 1482
Hpy99I CGWCG 2 cut(s) 293, 1193
HpyAV CCTTC 9 cut(s) 36, 56, 428, 541, 932, 1462, 1504, 1510, 1516
HpyCH4III ACNGT 3 cut(s) 905, 1102, 1486
HpyCH4IV ACGT 3 cut(s) 336, 1056, 1539
HpyCH4V TGCA 3 cut(s) 33, 738, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 567, 1574
HpyF3I CTNAG 3 cut(s) 723, 1261, 1422
HpySE526I ACGT 3 cut(s) 336, 1056, 1539
Hsp92I GRCGYC 1 cut(s) 1056
Hsp92II CATG 4 cut(s) 982, 1063, 1164, 1294
HspAI GCGC 1 cut(s) 192
Kpn2I TCCGGA 2 cut(s) 592, 707
Ksp22I TGATCA 1 cut(s) 615
KspI CCGCGG 2 cut(s) 422, 702
Kzo9I GATC 4 cut(s) 615, 771, 880, 1493
Lsp1109I GCAGC 3 cut(s) 456, 582, 1357
LweI GCATC 2 cut(s) 766, 1219
MaeI CTAG 2 cut(s) 783, 1268
MaeII ACGT 3 cut(s) 336, 1056, 1539
MaeIII GTNAC 3 cut(s) 280, 814, 1535
MalI GATC 4 cut(s) 617, 773, 882, 1495
MboI GATC 4 cut(s) 615, 771, 880, 1493
MfeI CAATTG 1 cut(s) 86
MhlI GDGCHC 3 cut(s) 659, 913, 1022
MluCI AATT 9 cut(s) 86, 317, 480, 541, 828, 874, 921, 1109, 1240
MluI ACGCGT 1 cut(s) 372
MlyI GAGTC 5 cut(s) 252, 287, 956, 977, 1322
MmeI TCCRAC 2 cut(s) 294, 1018
MroI TCCGGA 2 cut(s) 592, 707
MseI TTAA 1 cut(s) 1446
MspA1I CMGCKG 2 cut(s) 421, 701
MspI CCGG 5 cut(s) 12, 239, 593, 677, 708
MspR9I CCNGG 4 cut(s) 223, 240, 678, 1154
MunI CAATTG 1 cut(s) 86
MvaI CCWGG 2 cut(s) 223, 1154
MvnI CGCG 4 cut(s) 192, 374, 421, 701
MwoI GCNNNNNNNGC 3 cut(s) 218, 567, 1574
NciI CCSGG 2 cut(s) 240, 678
NcoI CCATGG 1 cut(s) 978
NdeII GATC 4 cut(s) 615, 771, 880, 1493
NlaIII CATG 4 cut(s) 982, 1063, 1164, 1294
NlaIV GGNNCC 5 cut(s) 591, 675, 729, 1473, 1558
NmeAIII GCCGAG 2 cut(s) 532, 638
NmuCI GTSAC 1 cut(s) 1535
NspV TTCGAA 1 cut(s) 545
PcsI WCGNNNNNNNCGW 1 cut(s) 869
PfeI GAWTC 7 cut(s) 971, 1130, 1145, 1214, 1247, 1307, 1328
PkrI GCNGC 7 cut(s) 55, 58, 275, 420, 471, 572, 1347
PleI GAGTC 5 cut(s) 251, 287, 956, 977, 1321
PpsI GAGTC 5 cut(s) 251, 287, 956, 977, 1321
PpuMI RGGWCCY 1 cut(s) 727
PsiI TTATAA 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 659
Psp5II RGGWCCY 1 cut(s) 727
Psp6I CCWGG 2 cut(s) 221, 1152
PspGI CCWGG 2 cut(s) 221, 1152
PspN4I GGNNCC 5 cut(s) 591, 675, 729, 1473, 1558
PspPI GGNCC 4 cut(s) 236, 727, 1040, 1557
PspPPI RGGWCCY 1 cut(s) 727
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SacI GAGCTC 1 cut(s) 659
SacII CCGCGG 2 cut(s) 422, 702
SaqAI TTAA 1 cut(s) 1446
SatI GCNGC 7 cut(s) 54, 57, 274, 419, 470, 571, 1346
Sau3AI GATC 4 cut(s) 615, 771, 880, 1493
Sau96I GGNCC 4 cut(s) 236, 727, 1040, 1557
SchI GAGTC 5 cut(s) 252, 287, 956, 977, 1322
ScrFI CCNGG 4 cut(s) 223, 240, 678, 1154
SduI GDGCHC 3 cut(s) 659, 913, 1022
SfaNI GCATC 2 cut(s) 766, 1219
Sfr303I CCGCGG 2 cut(s) 422, 702
SfuI TTCGAA 1 cut(s) 545
SgrBI CCGCGG 2 cut(s) 422, 702
SinI GGWCC 3 cut(s) 727, 1040, 1557
Sse9I AATT 9 cut(s) 86, 317, 480, 541, 828, 874, 921, 1109, 1240
SspMI CTAG 2 cut(s) 783, 1268
SstI GAGCTC 1 cut(s) 659
StyD4I CCNGG 4 cut(s) 221, 238, 676, 1152
StyI CCWWGG 1 cut(s) 978
TaaI ACNGT 3 cut(s) 905, 1102, 1486
TaiI ACGT 3 cut(s) 339, 1059, 1542
TaqI TCGA 6 cut(s) 455, 474, 545, 774, 960, 1340
TasI AATT 9 cut(s) 86, 317, 480, 541, 828, 874, 921, 1109, 1240
TauI GCSGC 4 cut(s) 56, 59, 276, 421
TfiI GAWTC 7 cut(s) 971, 1130, 1145, 1214, 1247, 1307, 1328
Tru1I TTAA 1 cut(s) 1446
Tru9I TTAA 1 cut(s) 1446
TscAI CASTG 2 cut(s) 1261, 1470
TseFI GTSAC 1 cut(s) 1535
TseI GCWGC 3 cut(s) 469, 570, 1345
Tsp45I GTSAC 1 cut(s) 1535
TspDTI ATGAA 8 cut(s) 410, 746, 1023, 1197, 1227, 1307, 1320, 1412
TspGWI ACGGA 3 cut(s) 280, 299, 1319
TspRI CASTG 2 cut(s) 1261, 1470
VpaK11BI GGWCC 3 cut(s) 727, 1040, 1557
XapI RAATTY 4 cut(s) 541, 828, 874, 1240
XcmI CCANNNNNNNNNTGG 2 cut(s) 985, 1240
XspI CTAG 2 cut(s) 783, 1268
ZraI GACGTC 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.