Rorug04G0353200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012534
Physical Location & Seq
Forward (+)
52514521 .. 52517842
3322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0353200.1

Sequence Viewer

Length: 1446 bp
ATGGAGGAGAGTTTGTTATTCCCAAGAGGCAAAGATCAAGCAGAGCAAAGAAAAGAGTCATGGGGTATGTTTTTCGAAGAAGTCAAGAGGTTGGGTTGCTTAGCCGGACCTATGGTTGCTGTCTTGCTATCCCAGTACATGGTGCAGGTTGTTTCGATGATGATGGTTGGTCACCTTGGTGAGCTTGCTCTCTCCAGCACTGCCTTGGCCATATCCTTTTCCGGGGTCACCGGTTTTAGTGTTCTTCTAGGAATGGCCAGTGCACTTGAAACTTTATGTGGGCAAGCGTATGGAGCTGAGCAATATCAGAAACTTGGACTTCAAACTTACACCGCCATATTTTCTCTCACCTTAGTTTGTCTTCCTTTGTCTTTGATATGGATATATATGGCGGATATACTGATGTTCATGGGCCAAGACCCTGTAATTTCACATGAAGCTGGCAAATTCACAATCTGGCTTCTTCCTGCTTTGTTTTCTTATGCAACTCTCCAACCACTCAATAGATACTTTCAGACTCAAAGTCTAATAACTCCTATGCTCGTGAGCTCTTTGGCGACTATTTTGTTCCATATTCCTCTCTGTTGGGCTCTAGTATTCAAGTCTGGACTGGATAGCCTTGGAGGGGCATTAGCAATGAGTATTTCCTACTGGTTCAATGCGATTGTACTTGGATTGTTCATGAAGTTTTCTTCCGCCTGTTCGAAAACCAGAGTTTCAATTTCTATGGAGCTATTCCATGGAATGAGAGAGTTCTTTTGTTTTGCCATCCCTTCTGCTGTAATGATATGCCTTGAGTGGTGGTCATTTGAGCTGCTTATCTTGCTGTCCGGGCTTTTACCAAACCCAGCTCTTGAAACTTCAGTTCTGTCTGTATGTCTCCAGACCATTGCGACACTCTATTCAATACCATACGGGTTTGGTGCTGCAGCAAGCACTAGAGTTTCAAATGAACTAGGTGCTGGCAACCCAAAAGGTGCTCGTATGGCTACGTGTGCTGCGATGTTTCTTGCACTCATAGAGACAACTATAATAACCACAACCCTCTTTGCCTGCCGCAATCTGTTTGGCTACAGTTTTAGTAATGAGAGAGATGTCGTCGATTACGTCACAACCATGGCTCCTCTAGTTTGTCTCTCTGTTATACTAGACAGTTTTCAAGGGGTCCTTTCAGGTATTGCTAGAGGATGTGGGTGGCAGCATATAGGGGCTTACGTAAACCTTGGAGCATTTTATTTATGTGGGATTCCTGTTGCTGCTACATTAGCTTTCTGGGTTCAACTAAGAGGAAGGGGTCTTTGGATTGGAATACAAGTTGGAGCTTTTGTGCAAACCACTCTGCTTGCTGTTGTAACAACTTGTACAAACTGGGAAAAGCAGGCAAGTAAGGCAAGGGAGAGGATATTTGAGGCAAGCTATGTAGAAGCTAATGGATTGTTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

481

Amino Acids

52.57

Weight (kDa)

6.1

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 37 - 196 2.1e-31 MatE
MatE PF01554 258 - 420 5.4e-28 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012534)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70200
fragaria_vesca FvH4_4g34980 FvH4_5g29371
malus_domestica MD16G1087500.v1.1
prunus_persica Prupe.1G259500_v2.0.a1
pyrus_communis pycom13g07510 pycom16g07510
rosa_chinensis RchiOBHm_Chr4g0444501
rosa_laevigata RLG00000005829
rosa_multiflora Rmu_co8304031.1_g000001 Rmu_sc0003839.1_g000008 Rmu_sc0035747.1_g000002
rosa_roxburghii Rroxscaffold_5G00384980
rosa_rugosa Rorug04G0353200
rosa_samantha Rh4AG413400 Rh4BG424900 Rh4CG439500 Rh4DG420400
rosa_wichuraiana Rw4G035510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 139
AciI CCGC 4 cut(s) 333, 392, 696, 1057
AcoI YGGCCR 2 cut(s) 207, 255
AcsI RAATTY 1 cut(s) 446
AcuI CTGAAG 1 cut(s) 846
AfaI GTAC 3 cut(s) 137, 669, 1363
AfiI CCNNNNNNNGG 3 cut(s) 139, 222, 625
AflIII ACRYGT 1 cut(s) 992
AgeI ACCGGT 1 cut(s) 230
AhdI GACNNNNNGTC 1 cut(s) 522
AjuI GAANNNNNNNTTGG 2 cut(s) 1282, 1314
AleI CACNNNNGTG 1 cut(s) 177
Alw21I GWGCWC 3 cut(s) 265, 551, 982
Alw26I GTCTC 3 cut(s) 884, 1016, 1139
Alw44I GTGCAC 1 cut(s) 261
AoxI GGCC 3 cut(s) 207, 255, 412
ApaLI GTGCAC 1 cut(s) 261
ApeKI GCWGC 6 cut(s) 814, 926, 929, 998, 1198, 1256
ApoI RAATTY 1 cut(s) 446
AsiGI ACCGGT 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 107, 412, 1165
AsuC2I CCSGG 2 cut(s) 223, 832
AsuHPI GGTGA 4 cut(s) 164, 191, 220, 340
AsuII TTCGAA 2 cut(s) 75, 704
AvaII GGWCC 2 cut(s) 107, 1165
BaeGI GKGCMC 1 cut(s) 265
BalI TGGCCA 2 cut(s) 209, 257
BanII GRGCYC 2 cut(s) 551, 592
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 3 cut(s) 265, 551, 982
BbvI GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
BccI CCATC 2 cut(s) 157, 776
BcnI CCSGG 2 cut(s) 223, 832
BcoDI GTCTC 3 cut(s) 884, 1016, 1139
BfaI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
BfmI CTRYAG 2 cut(s) 927, 1072
BfuAI ACCTGC 1 cut(s) 136
BisI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
BlpI GCTNAGC 2 cut(s) 100, 297
BlsI GCNGC 7 cut(s) 816, 928, 931, 1000, 1058, 1200, 1258
Bme1390I CCNGG 2 cut(s) 223, 832
Bme18I GGWCC 2 cut(s) 107, 1165
BmeRI GACNNNNNGTC 1 cut(s) 522
BmgT120I GGNCC 3 cut(s) 107, 412, 1165
BmiI GGNNCC 2 cut(s) 1122, 1166
BmrFI CCNGG 2 cut(s) 223, 832
BmrI ACTGGG 2 cut(s) 127, 1378
BmuI ACTGGG 2 cut(s) 127, 1378
BpiI GAAGAC 1 cut(s) 353
BpmI CTGGAG 2 cut(s) 178, 866
Bpu1102I GCTNAGC 2 cut(s) 100, 297
Bpu14I TTCGAA 2 cut(s) 75, 704
BpuEI CTTGAG 1 cut(s) 815
BpuMI CCSGG 2 cut(s) 223, 832
BsaAI YACGTR 2 cut(s) 993, 1216
BsaJI CCNNGG 7 cut(s) 175, 204, 222, 619, 739, 1116, 1222
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 5 cut(s) 29, 1105, 1135, 1311, 1341
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 222, 625
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 5 cut(s) 133, 258, 615, 656, 1373
Bse3DI GCAATG 2 cut(s) 642, 888
BseDI CCNNGG 7 cut(s) 175, 204, 222, 619, 739, 1116, 1222
BseGI GGATG 2 cut(s) 768, 1193
BseLI CCNNNNNNNGG 3 cut(s) 139, 222, 625
BseMI GCAATG 2 cut(s) 642, 888
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 5 cut(s) 133, 258, 615, 656, 1373
BseRI GAGGAG 2 cut(s) 20, 1113
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
BseYI CCCAGC 1 cut(s) 847
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 3 cut(s) 209, 257, 414
BshTI ACCGGT 1 cut(s) 230
BsiHKAI GWGCWC 3 cut(s) 265, 551, 982
BsiSI CCGG 4 cut(s) 105, 222, 231, 831
BslI CCNNNNNNNGG 3 cut(s) 139, 222, 625
BsmAI GTCTC 3 cut(s) 884, 1016, 1139
BsnI GGCC 3 cut(s) 209, 257, 414
Bsp119I TTCGAA 2 cut(s) 75, 704
Bsp1286I GDGCHC 4 cut(s) 265, 551, 592, 982
Bsp1407I TGTACA 1 cut(s) 1361
Bsp143I GATC 1 cut(s) 34
Bsp1720I GCTNAGC 2 cut(s) 100, 297
Bsp19I CCATGG 2 cut(s) 739, 1116
BspACI CCGC 4 cut(s) 333, 392, 696, 1057
BspANI GGCC 3 cut(s) 209, 257, 414
BspCNI CTCAG 1 cut(s) 289
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 2 cut(s) 1122, 1166
BspMAI CTGCAG 1 cut(s) 931
BspMI ACCTGC 1 cut(s) 136
BspT104I TTCGAA 2 cut(s) 75, 704
BsrDI GCAATG 2 cut(s) 642, 888
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 1361
BsrI ACTGG 5 cut(s) 133, 258, 615, 656, 1373
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 7 cut(s) 175, 204, 222, 619, 739, 1116, 1222
BssMI GATC 1 cut(s) 34
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
Bst2BI CACGAG 1 cut(s) 542
Bst4CI ACNGT 2 cut(s) 1076, 1154
BstAUI TGTACA 1 cut(s) 1361
BstBAI YACGTR 2 cut(s) 993, 1216
BstBI TTCGAA 2 cut(s) 75, 704
BstC8I GCNNGC 9 cut(s) 186, 285, 442, 934, 964, 1054, 1344, 1380, 1414
BstDEI CTNAG 4 cut(s) 100, 297, 352, 1283
BstDSI CCRYGG 2 cut(s) 739, 1116
BstEII GGTNACC 2 cut(s) 170, 226
BstF5I GGATG 2 cut(s) 768, 1193
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 3 cut(s) 884, 1016, 1139
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 7 cut(s) 293, 823, 832, 986, 995, 1265, 1388
BstPI GGTNACC 2 cut(s) 170, 226
BstSCI CCNGG 2 cut(s) 221, 830
BstSFI CTRYAG 2 cut(s) 927, 1072
BstSLI GKGCMC 1 cut(s) 265
BstSNI TACGTA 1 cut(s) 1216
BstV1I GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 3 cut(s) 209, 257, 414
BtgI CCRYGG 2 cut(s) 739, 1116
BtgZI GCGATG 1 cut(s) 1016
BtsCI GGATG 2 cut(s) 768, 1193
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 198, 265
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 9 cut(s) 186, 285, 442, 934, 964, 1054, 1344, 1380, 1414
CciI TCATGA 1 cut(s) 681
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 107, 412, 1165
Csp6I GTAC 3 cut(s) 136, 668, 1362
CspAI ACCGGT 1 cut(s) 230
CspCI CAANNNNNGTGG 2 cut(s) 1324, 1359
CviAII CATG 7 cut(s) 60, 139, 409, 434, 682, 740, 1117
CviQI GTAC 3 cut(s) 136, 668, 1362
DdeI CTNAG 4 cut(s) 100, 297, 352, 1283
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
DriI GACNNNNNGTC 1 cut(s) 522
EaeI YGGCCR 2 cut(s) 207, 255
Eam1105I GACNNNNNGTC 1 cut(s) 522
EciI GGCGGA 2 cut(s) 407, 685
Ecl136II GAGCTC 1 cut(s) 549
Eco105I TACGTA 1 cut(s) 1216
Eco130I CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
Eco24I GRGCYC 2 cut(s) 551, 592
Eco47I GGWCC 2 cut(s) 107, 1165
Eco53kI GAGCTC 1 cut(s) 549
Eco57I CTGAAG 1 cut(s) 846
Eco91I GGTNACC 2 cut(s) 170, 226
EcoICRI GAGCTC 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 1165
EcoO65I GGTNACC 2 cut(s) 170, 226
EcoT14I CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
EcoT38I GRGCYC 2 cut(s) 551, 592
ErhI CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
FaeI CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
FalI AAGNNNNNCTT 2 cut(s) 1152, 1184
FatI CATG 7 cut(s) 59, 138, 408, 433, 681, 739, 1116
Fnu4HI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
FokI GGATG 2 cut(s) 755, 1200
FriOI GRGCYC 2 cut(s) 551, 592
Fsp4HI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
FspBI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
GluI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 2 cut(s) 178, 866
HaeIII GGCC 3 cut(s) 209, 257, 414
HapII CCGG 4 cut(s) 105, 222, 231, 831
Hin1II CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
HinfI GANTC 3 cut(s) 56, 517, 1246
HpaII CCGG 4 cut(s) 105, 222, 231, 831
HphI GGTGA 4 cut(s) 164, 191, 220, 340
Hpy166II GTNNAC 2 cut(s) 263, 1219
Hpy188I TCNGA 2 cut(s) 309, 516
Hpy188III TCNNGA 6 cut(s) 85, 544, 606, 682, 854, 883
Hpy8I GTNNAC 2 cut(s) 263, 1219
Hpy99I CGWCG 1 cut(s) 1103
HpyAV CCTTC 2 cut(s) 783, 1284
HpyCH4III ACNGT 2 cut(s) 1076, 1154
HpyCH4IV ACGT 3 cut(s) 992, 1107, 1215
HpyCH4V TGCA 7 cut(s) 145, 263, 485, 929, 1013, 1330, 1441
HpyF10VI GCNNNNNNNGC 7 cut(s) 293, 823, 832, 986, 995, 1265, 1388
HpyF3I CTNAG 4 cut(s) 100, 297, 352, 1283
HpySE526I ACGT 3 cut(s) 992, 1107, 1215
Hsp92II CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 5 cut(s) 293, 730, 1126, 1226, 1319
Lsp1109I GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
MaeI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
MaeII ACGT 3 cut(s) 992, 1107, 1215
MaeIII GTNAC 4 cut(s) 170, 226, 1108, 1351
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 5 cut(s) 89, 236, 353, 455, 684
MhlI GDGCHC 4 cut(s) 265, 551, 592, 982
MlsI TGGCCA 2 cut(s) 209, 257
MluCI AATT 3 cut(s) 426, 446, 720
MluNI TGGCCA 2 cut(s) 209, 257
MlyI GAGTC 2 cut(s) 65, 511
MmeI TCCRAC 2 cut(s) 517, 1297
Mox20I TGGCCA 2 cut(s) 209, 257
MscI TGGCCA 2 cut(s) 209, 257
MslI CAYNNNNRTG 2 cut(s) 177, 1115
Msp20I TGGCCA 2 cut(s) 209, 257
MspI CCGG 4 cut(s) 105, 222, 231, 831
MspR9I CCNGG 2 cut(s) 223, 832
MwoI GCNNNNNNNGC 7 cut(s) 293, 823, 832, 986, 995, 1265, 1388
NciI CCSGG 2 cut(s) 223, 832
NcoI CCATGG 2 cut(s) 739, 1116
NdeII GATC 1 cut(s) 34
NlaIII CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
NlaIV GGNNCC 2 cut(s) 1122, 1166
NmuCI GTSAC 3 cut(s) 170, 226, 1108
NspV TTCGAA 2 cut(s) 75, 704
OliI CACNNNNGTG 1 cut(s) 177
PagI TCATGA 1 cut(s) 681
PcsI WCGNNNNNNNCGW 2 cut(s) 998, 1104
PfeI GAWTC 1 cut(s) 1246
PflMI CCANNNNNTGG 1 cut(s) 139
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 7 cut(s) 816, 928, 931, 1000, 1058, 1200, 1258
PleI GAGTC 2 cut(s) 64, 511
PpsI GAGTC 2 cut(s) 64, 511
Ppu21I YACGTR 2 cut(s) 993, 1216
PpuMI RGGWCCY 1 cut(s) 1165
Psp124BI GAGCTC 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 1165
PspEI GGTNACC 2 cut(s) 170, 226
PspFI CCCAGC 1 cut(s) 847
PspN4I GGNNCC 2 cut(s) 1122, 1166
PspPI GGNCC 3 cut(s) 107, 412, 1165
PspPPI RGGWCCY 1 cut(s) 1165
PstI CTGCAG 1 cut(s) 931
RsaI GTAC 3 cut(s) 137, 669, 1363
RsaNI GTAC 3 cut(s) 136, 668, 1362
RseI CAYNNNNRTG 2 cut(s) 177, 1115
SacI GAGCTC 1 cut(s) 551
SatI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 3 cut(s) 107, 412, 1165
SchI GAGTC 2 cut(s) 65, 511
ScrFI CCNGG 2 cut(s) 223, 832
SduI GDGCHC 4 cut(s) 265, 551, 592, 982
SfcI CTRYAG 2 cut(s) 927, 1072
SfuI TTCGAA 2 cut(s) 75, 704
SinI GGWCC 2 cut(s) 107, 1165
SmiMI CAYNNNNRTG 2 cut(s) 177, 1115
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
SnaBI TACGTA 1 cut(s) 1216
Sse9I AATT 3 cut(s) 426, 446, 720
SsiI CCGC 4 cut(s) 333, 392, 696, 1057
SspMI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
SstI GAGCTC 1 cut(s) 551
StyD4I CCNGG 2 cut(s) 221, 830
StyI CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
TaaI ACNGT 2 cut(s) 1076, 1154
TaiI ACGT 3 cut(s) 995, 1110, 1218
TaqI TCGA 4 cut(s) 75, 155, 704, 1101
TasI AATT 3 cut(s) 426, 446, 720
TatI WGTACW 3 cut(s) 135, 667, 1361
TauI GCSGC 1 cut(s) 1059
TfiI GAWTC 1 cut(s) 1246
TscAI CASTG 2 cut(s) 205, 265
TseFI GTSAC 3 cut(s) 170, 226, 1108
TseI GCWGC 6 cut(s) 814, 926, 929, 998, 1198, 1256
Tsp45I GTSAC 3 cut(s) 170, 226, 1108
TspDTI ATGAA 5 cut(s) 397, 450, 670, 698, 966
TspRI CASTG 2 cut(s) 205, 265
Van91I CCANNNNNTGG 1 cut(s) 139
VneI GTGCAC 1 cut(s) 261
VpaK11BI GGWCC 2 cut(s) 107, 1165
XapI RAATTY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XspI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.