MD16G1095300.v1.1

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013538
Physical Location & Seq
Reverse (-)
6590746 .. 6593341
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1095300.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGGCAGAGAGCTCAGAGCAGGACCTCATGACCACCGCGAGTAGGAGGTTGGCCTCCATCGCCAACCACCTCAGTCCGGCCCATCTATCTCCCAGCTCCGCCGCAATTTCTCTATCCAACGCCTCCATGAACGACAGCTACCACCGCACCCACGGCGAGGTCTCGACTCACCAAGTTGTTTGGAAAAATGCTCGCGACGAGTCTGGCGAGGAATTCACCGACATTATTTATGAGAAATCCGTCGGCGAAGCCATTGCAAAGATTACGATTAATAGGCCGGAGAGAAGAAACGCGTTTCGGCCGCAGACGGTTAAGGAGCTCATTCGCGCGTTTAACGATGCCAGGGACGACGGCTCCATAGGCGTCGTCATTCTTACCGGGAAGGGAACCAAGGCATTCTGCAGTGGCGGTGATCAGGCTTTGAGAGGCAAAGATGGATATTCTGATTACGAAGATTTTGGTCGTCTTAATGTTTTGGATTTGCAGGTCCAGATTCGACGTCTTCCAAAGCCAGTAATAGCCATGGTGGCTGGTTATGCAGTTGGGGGAGGACATGTATTACACATGGTCTGTGATCTAACCATTGCAGCAGACAATGCTATTTTTGGTCAGACCGGTCCTAAGGTTGGAAGCTTTGACGCTGGTTATGGGAGTTCAATCATGTCCCGTTTGATCGGGCCTAAAAAAGCACGAGAAATGTGGTTTATGGCAAGGTTTTACACAGCCGCTGAAGCTGAGAAAATGGGTCTTGTTAACACTGTTGTACCGCTGGAGAGTTTAGAGCAAGAAACGGTTAAATGGTGTCGAGAAATGCTAAGAAACAGCCCAACTGCACTTCGGGTGCTGAAATCTGCTCTTAATGCAGTTGACGATGGTCATGCTGGACTACAGCAACTTGCTGGAGATGCCACACTGTTATTCTATGGCACTGAGGAAGGGAATGAAGGGAAGACAGCTTATATGCAACACAGGCGTCCGGATTTCTCAAAGTTTCCCAGGAGACCTTAA

Protein Analysis

336

Amino Acids

36.71

Weight (kDa)

7.13

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 83 - 329 1e-74 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 86 - 262 1.3e-21 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60550
fragaria_vesca FvH4_4g34130 FvH4_4g34130 FvH4_4g34130
malus_domestica MD13G1094000.v1.1 MD16G1095300.v1.1
prunus_persica Prupe.1G251300_v2.0.a1
pyrus_communis pycom13g08190
rosa_chinensis RchiOBHm_Chr4g0443641
rosa_laevigata RLG00000005906
rosa_multiflora Rmu_sc0001118.1_g000011 Rmu_sc0033917.1_g000002
rosa_roxburghii Rroxscaffold_5G00384190
rosa_samantha Rh4AG405800 Rh4BG416800 Rh4CG431300 Rh4DG412500
rosa_wichuraiana Rw4G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 502
Acc36I ACCTGC 1 cut(s) 475
AccII CGCG 5 cut(s) 38, 195, 293, 327, 329
AccIII TCCGGA 1 cut(s) 976
AciI CCGC 8 cut(s) 36, 99, 102, 145, 302, 408, 726, 767
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 750
AcyI GRCGYC 3 cut(s) 363, 499, 973
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 4 cut(s) 42, 76, 157, 626
AflIII ACRYGT 2 cut(s) 291, 553
AgeI ACCGGT 1 cut(s) 614
AgsI TTSAA 1 cut(s) 657
AjnI CCWGG 2 cut(s) 341, 995
AluBI AGCT 7 cut(s) 12, 96, 138, 319, 633, 734, 956
AluI AGCT 7 cut(s) 12, 96, 138, 319, 633, 734, 956
Alw21I GWGCWC 2 cut(s) 14, 321
Alw26I GTCTC 2 cut(s) 166, 994
AlwNI CAGNNNCTG 1 cut(s) 728
Aor13HI TCCGGA 1 cut(s) 976
AoxI GGCC 5 cut(s) 51, 78, 275, 299, 677
ApeKI GCWGC 1 cut(s) 587
ApoI RAATTY 1 cut(s) 212
AseI ATTAAT 1 cut(s) 270
AsiGI ACCGGT 1 cut(s) 614
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 5 cut(s) 22, 79, 487, 617, 677
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 3 cut(s) 161, 208, 422
AvaII GGWCC 3 cut(s) 22, 487, 617
AxyI CCTNAGG 1 cut(s) 621
BaeI ACNNNNGTAYC 2 cut(s) 747, 780
BanII GRGCYC 2 cut(s) 14, 321
BauI CACGAG 1 cut(s) 690
BbsI GAAGAC 2 cut(s) 494, 956
Bbv12I GWGCWC 2 cut(s) 14, 321
BbvI GCAGC 1 cut(s) 599
BccI CCATC 4 cut(s) 65, 90, 428, 866
BceAI ACGGC 2 cut(s) 169, 367
BcgI CGANNNNNNTGC 4 cut(s) 236, 270, 860, 894
BciT130I CCWGG 2 cut(s) 343, 997
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 166, 994
BfmI CTRYAG 2 cut(s) 400, 887
BfuAI ACCTGC 1 cut(s) 475
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 4 cut(s) 102, 302, 588, 726
BlsI GCNGC 4 cut(s) 103, 303, 589, 727
Bme1390I CCNGG 3 cut(s) 343, 379, 997
Bme18I GGWCC 3 cut(s) 22, 487, 617
BmgT120I GGNCC 5 cut(s) 22, 79, 487, 617, 677
BmiI GGNNCC 2 cut(s) 355, 388
BmrFI CCNGG 3 cut(s) 343, 379, 997
BmsI GCATC 2 cut(s) 328, 895
BoxI GACNNNNGTC 1 cut(s) 873
BpiI GAAGAC 2 cut(s) 494, 956
BpmI CTGGAG 2 cut(s) 791, 921
BpuMI CCSGG 1 cut(s) 379
BsaHI GRCGYC 3 cut(s) 363, 499, 973
BsaI GGTCTC 2 cut(s) 166, 994
BsaJI CCNNGG 5 cut(s) 151, 342, 390, 522, 995
BsaWI WCCGGW 2 cut(s) 614, 976
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 540, 570
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 76, 157, 626
Bse118I RCCGGY 1 cut(s) 614
Bse1I ACTGG 1 cut(s) 512
Bse21I CCTNAGG 1 cut(s) 621
Bse3DI GCAATG 2 cut(s) 252, 582
BseAI TCCGGA 1 cut(s) 976
BseBI CCWGG 2 cut(s) 343, 997
BseDI CCNNGG 5 cut(s) 151, 342, 390, 522, 995
BseLI CCNNNNNNNGG 4 cut(s) 42, 76, 157, 626
BseMI GCAATG 2 cut(s) 252, 582
BseMII CTCAG 4 cut(s) 27, 85, 726, 921
BseNI ACTGG 1 cut(s) 512
BseX3I CGGCCG 1 cut(s) 299
BseXI GCAGC 1 cut(s) 599
BseYI CCCAGC 1 cut(s) 92
BsgI GTGCAG 1 cut(s) 816
Bsh1236I CGCG 5 cut(s) 38, 195, 293, 327, 329
Bsh1285I CGRYCG 1 cut(s) 302
BshFI GGCC 5 cut(s) 53, 80, 277, 301, 679
BshTI ACCGGT 1 cut(s) 614
BsiEI CGRYCG 1 cut(s) 302
BsiHKAI GWGCWC 2 cut(s) 14, 321
BsiSI CCGG 5 cut(s) 77, 278, 378, 615, 977
BslFI GGGAC 2 cut(s) 359, 649
BslI CCNNNNNNNGG 4 cut(s) 42, 76, 157, 626
BsmAI GTCTC 2 cut(s) 166, 994
BsmFI GGGAC 2 cut(s) 359, 649
BsmI GAATGC 1 cut(s) 395
BsnI GGCC 5 cut(s) 53, 80, 277, 301, 679
Bso31I GGTCTC 2 cut(s) 166, 994
Bsp1286I GDGCHC 2 cut(s) 14, 321
Bsp13I TCCGGA 1 cut(s) 976
Bsp143I GATC 3 cut(s) 412, 574, 672
Bsp19I CCATGG 1 cut(s) 522
Bsp68I TCGCGA 1 cut(s) 195
BspACI CCGC 8 cut(s) 36, 99, 102, 145, 302, 408, 726, 767
BspANI GGCC 5 cut(s) 53, 80, 277, 301, 679
BspCNI CTCAG 4 cut(s) 26, 84, 727, 922
BspEI TCCGGA 1 cut(s) 976
BspFNI CGCG 5 cut(s) 38, 195, 293, 327, 329
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 355, 388
BspMAI CTGCAG 1 cut(s) 404
BspMI ACCTGC 1 cut(s) 475
BspTNI GGTCTC 2 cut(s) 166, 994
BsrDI GCAATG 2 cut(s) 252, 582
BsrFI RCCGGY 1 cut(s) 614
BsrI ACTGG 1 cut(s) 512
BssAI RCCGGY 1 cut(s) 614
BssECI CCNNGG 5 cut(s) 151, 342, 390, 522, 995
BssMI GATC 3 cut(s) 412, 574, 672
BssNI GRCGYC 3 cut(s) 363, 499, 973
BssSI CACGAG 1 cut(s) 690
BssT1I CCWWGG 2 cut(s) 390, 522
Bst2BI CACGAG 1 cut(s) 690
Bst2UI CCWGG 2 cut(s) 343, 997
Bst4CI ACNGT 4 cut(s) 310, 760, 793, 915
BstACI GRCGYC 3 cut(s) 363, 499, 973
BstAPI GCANNNNNTGC 1 cut(s) 596
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 6 cut(s) 13, 71, 621, 735, 815, 930
BstDSI CCRYGG 2 cut(s) 151, 522
BstFNI CGCG 5 cut(s) 38, 195, 293, 327, 329
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 3 cut(s) 415, 577, 675
BstMAI GTCTC 2 cut(s) 166, 994
BstMBI GATC 3 cut(s) 412, 574, 672
BstMCI CGRYCG 1 cut(s) 302
BstNI CCWGG 2 cut(s) 343, 997
BstNSI RCATGY 1 cut(s) 557
BstPAI GACNNNNGTC 1 cut(s) 873
BstSCI CCNGG 3 cut(s) 341, 377, 995
BstSFI CTRYAG 2 cut(s) 400, 887
BstUI CGCG 5 cut(s) 38, 195, 293, 327, 329
BstV1I GCAGC 1 cut(s) 599
BstV2I GAAGAC 2 cut(s) 494, 956
BstZI CGGCCG 1 cut(s) 299
Bsu36I CCTNAGG 1 cut(s) 621
BsuRI GGCC 5 cut(s) 53, 80, 277, 301, 679
BtgI CCRYGG 2 cut(s) 151, 522
BtgZI GCGATG 1 cut(s) 43
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 4 cut(s) 409, 756, 911, 927
BtuMI TCGCGA 1 cut(s) 195
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 193
CaiI CAGNNNCTG 1 cut(s) 728
CciI TCATGA 1 cut(s) 27
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 5 cut(s) 22, 79, 487, 617, 677
CseI GACGC 3 cut(s) 352, 647, 962
Csp6I GTAC 1 cut(s) 764
CspAI ACCGGT 1 cut(s) 614
CviAII CATG 7 cut(s) 28, 127, 523, 554, 565, 661, 878
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 6 cut(s) 13, 71, 621, 735, 815, 930
DpnI GATC 3 cut(s) 414, 576, 674
DpnII GATC 3 cut(s) 412, 574, 672
EaeI YGGCCR 1 cut(s) 299
EagI CGGCCG 1 cut(s) 299
EciI GGCGGA 1 cut(s) 88
Ecl136II GAGCTC 2 cut(s) 12, 319
EclXI CGGCCG 1 cut(s) 299
Eco130I CCWWGG 2 cut(s) 390, 522
Eco24I GRGCYC 2 cut(s) 14, 321
Eco31I GGTCTC 2 cut(s) 166, 994
Eco47I GGWCC 3 cut(s) 22, 487, 617
Eco52I CGGCCG 1 cut(s) 299
Eco53kI GAGCTC 2 cut(s) 12, 319
Eco57I CTGAAG 1 cut(s) 750
Eco81I CCTNAGG 1 cut(s) 621
EcoICRI GAGCTC 2 cut(s) 12, 319
EcoO109I RGGNCCY 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 341, 995
EcoT14I CCWWGG 2 cut(s) 390, 522
EcoT38I GRGCYC 2 cut(s) 14, 321
ErhI CCWWGG 2 cut(s) 390, 522
FaeI CATG 7 cut(s) 31, 130, 526, 557, 568, 664, 881
FaqI GGGAC 2 cut(s) 359, 649
FatI CATG 7 cut(s) 27, 126, 522, 553, 564, 660, 877
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 4 cut(s) 102, 302, 588, 726
FriOI GRGCYC 2 cut(s) 14, 321
Fsp4HI GCNGC 4 cut(s) 102, 302, 588, 726
GlaI GCGC 1 cut(s) 328
GluI GCNGC 4 cut(s) 102, 302, 588, 726
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 2 cut(s) 791, 921
HaeIII GGCC 5 cut(s) 53, 80, 277, 301, 679
HapII CCGG 5 cut(s) 77, 278, 378, 615, 977
HgaI GACGC 3 cut(s) 352, 647, 962
HhaI GCGC 1 cut(s) 329
Hin1I GRCGYC 3 cut(s) 363, 499, 973
Hin1II CATG 7 cut(s) 31, 130, 526, 557, 568, 664, 881
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 2 cut(s) 754, 868
HindII GTYRAC 2 cut(s) 754, 868
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 3 cut(s) 166, 200, 493
HpaI GTTAAC 1 cut(s) 754
HpaII CCGG 5 cut(s) 77, 278, 378, 615, 977
HphI GGTGA 3 cut(s) 161, 208, 422
Hpy166II GTNNAC 2 cut(s) 754, 868
Hpy188I TCNGA 3 cut(s) 16, 445, 612
Hpy188III TCNNGA 6 cut(s) 28, 163, 194, 490, 806, 977
Hpy8I GTNNAC 2 cut(s) 754, 868
Hpy99I CGWCG 5 cut(s) 200, 245, 353, 368, 501
HpyAV CCTTC 3 cut(s) 376, 929, 938
HpyCH4III ACNGT 4 cut(s) 310, 760, 793, 915
HpyCH4IV ACGT 1 cut(s) 499
HpyCH4V TGCA 8 cut(s) 257, 402, 484, 539, 587, 833, 863, 964
HpyF3I CTNAG 6 cut(s) 13, 71, 621, 735, 815, 930
HpySE526I ACGT 1 cut(s) 499
Hsp92I GRCGYC 3 cut(s) 363, 499, 973
Hsp92II CATG 7 cut(s) 31, 130, 526, 557, 568, 664, 881
HspAI GCGC 1 cut(s) 327
Kpn2I TCCGGA 1 cut(s) 976
Ksp22I TGATCA 1 cut(s) 412
KspAI GTTAAC 1 cut(s) 754
Kzo9I GATC 3 cut(s) 412, 574, 672
LmnI GCTCC 3 cut(s) 101, 316, 359
Lsp1109I GCAGC 1 cut(s) 599
LweI GCATC 2 cut(s) 328, 895
MaeII ACGT 1 cut(s) 499
MalI GATC 3 cut(s) 414, 576, 674
MboI GATC 3 cut(s) 412, 574, 672
MboII GAAGA 4 cut(s) 297, 464, 494, 961
MhlI GDGCHC 2 cut(s) 14, 321
MluCI AATT 2 cut(s) 105, 212
MluI ACGCGT 1 cut(s) 291
MlyI GAGTC 2 cut(s) 160, 209
MmeI TCCRAC 2 cut(s) 141, 607
MroI TCCGGA 1 cut(s) 976
MseI TTAA 8 cut(s) 270, 312, 333, 468, 753, 795, 858, 1006
MspA1I CMGCKG 2 cut(s) 728, 769
MspI CCGG 5 cut(s) 77, 278, 378, 615, 977
MspR9I CCNGG 3 cut(s) 343, 379, 997
Mva1269I GAATGC 1 cut(s) 395
MvaI CCWGG 2 cut(s) 343, 997
MvnI CGCG 5 cut(s) 38, 195, 293, 327, 329
NciI CCSGG 1 cut(s) 379
NcoI CCATGG 1 cut(s) 522
NdeII GATC 3 cut(s) 412, 574, 672
NlaIII CATG 7 cut(s) 31, 130, 526, 557, 568, 664, 881
NlaIV GGNNCC 2 cut(s) 355, 388
NruI TCGCGA 1 cut(s) 195
NspI RCATGY 1 cut(s) 557
PagI TCATGA 1 cut(s) 27
PciI ACATGT 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 493
PinAI ACCGGT 1 cut(s) 614
PkrI GCNGC 4 cut(s) 103, 303, 589, 727
PleI GAGTC 2 cut(s) 160, 208
PpsI GAGTC 2 cut(s) 160, 208
PpuMI RGGWCCY 1 cut(s) 22
PscI ACATGT 1 cut(s) 553
PshAI GACNNNNGTC 1 cut(s) 873
PshBI ATTAAT 1 cut(s) 270
Psp124BI GAGCTC 2 cut(s) 14, 321
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 2 cut(s) 341, 995
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 2 cut(s) 341, 995
PspN4I GGNNCC 2 cut(s) 355, 388
PspPI GGNCC 5 cut(s) 22, 79, 487, 617, 677
PspPPI RGGWCCY 1 cut(s) 22
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
PstI CTGCAG 1 cut(s) 404
PstNI CAGNNNCTG 1 cut(s) 728
RruI TCGCGA 1 cut(s) 195
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
SacI GAGCTC 2 cut(s) 14, 321
SaqAI TTAA 8 cut(s) 270, 312, 333, 468, 753, 795, 858, 1006
SatI GCNGC 4 cut(s) 102, 302, 588, 726
Sau3AI GATC 3 cut(s) 412, 574, 672
Sau96I GGNCC 5 cut(s) 22, 79, 487, 617, 677
SchI GAGTC 2 cut(s) 160, 209
ScrFI CCNGG 3 cut(s) 343, 379, 997
SduI GDGCHC 2 cut(s) 14, 321
SfaNI GCATC 2 cut(s) 328, 895
SfcI CTRYAG 2 cut(s) 400, 887
SinI GGWCC 3 cut(s) 22, 487, 617
Sse9I AATT 2 cut(s) 105, 212
SsiI CCGC 8 cut(s) 36, 99, 102, 145, 302, 408, 726, 767
SstI GAGCTC 2 cut(s) 14, 321
StyD4I CCNGG 3 cut(s) 341, 377, 995
StyI CCWWGG 2 cut(s) 390, 522
TaaI ACNGT 4 cut(s) 310, 760, 793, 915
TaiI ACGT 1 cut(s) 502
TaqI TCGA 3 cut(s) 164, 496, 805
TasI AATT 2 cut(s) 105, 212
TauI GCSGC 3 cut(s) 104, 304, 728
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 8 cut(s) 270, 312, 333, 468, 753, 795, 858, 1006
Tru9I TTAA 8 cut(s) 270, 312, 333, 468, 753, 795, 858, 1006
TscAI CASTG 4 cut(s) 409, 763, 918, 934
TseI GCWGC 1 cut(s) 587
TspDTI ATGAA 2 cut(s) 143, 957
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 4 cut(s) 409, 763, 918, 934
VpaK11BI GGWCC 3 cut(s) 22, 487, 617
VspI ATTAAT 1 cut(s) 270
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 557
ZraI GACGTC 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.