Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0013538

Physical Location & Seq

Genomic Coordinates Forward (+)

67175290 .. 67177952

Gene Structure 2663 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG431300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 840 bp

ATGGCTCAAAGTACAGAGTCAGAGAAGGACCTCATGAACAGAGTGAACAGAAGAATGGCCTCCATCTCCAACCATCTGACTCCGATCAATTCAATTCCCAACTCCATTACTCTGTCGCACACATCTTCCATGGACGACAGCTACCACGGGACCCACGGCCAAGTCCCGACTCACCAAGTTGTCTGGGAAGCTGCTGTTGACGAGTCCGGCAAGGAGTTCACTGACATTATCTACGAGAAATCCGTCGGCGAAGGAATAGCAAAGATTACGATTAATAGGCCGGAGAGAAGGAATGCGTTCCGGCCGCAGACGATCAAGGAGCTGATCCGGGCTTTTAATGATGCCAGGGACGACAGTTCCATTGGCGTTATTGTTCTTACTGGGAAGGGAACCAAGGCATTTTGTAGCGGTGGTGATCAGGCTTTGAGAAGCACGGATGGTTATTCTGATTTTGAAAATTTTGGTCGTCTTAATGTTTTAGACCTTCAGGTTCAGATTCGTCGTCTTCCAAAGCCAGTAATTGCCATGGTGGCTGGTTATGCTGTTGGGGGAGGGCATGTGTTGCACATGGTATGTGATCTAACCATTGCAGCAGACAATGCTATTTTTGGTCAAACTGGCCCTAAGGTTGGAAGCTTTGATGCTGGTTATGGGAGTTCGATCATGTCCCGCTTGGTCGGGCCTAAAAAAGCACGTGAAATGTGGTTTCTAGCAAGGTTTTACACAGCTGCTGAAGCAGAGAAAATGGGACTTGTTAACACTGTTGTTCCGGTAAGTTCATCATCAATGTTACGGAACATTACTTCTGATGGTGTGTGTCTAATATATTCGATCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0008935 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016829 GO:0016830 GO:0016833 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051188 GO:0071704 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

ECH_1

Protein Analysis

279

Amino Acids

30.38

Weight (kDa)

7.06

Isoelectric Point (pI)

37.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	84 - 258	1.8e-60	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	87 - 264	1.7e-21	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013538)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60550
fragaria_vesca	FvH4_4g34130 FvH4_4g34130 FvH4_4g34130
malus_domestica	MD13G1094000.v1.1 MD16G1095300.v1.1
prunus_persica	Prupe.1G251300_v2.0.a1
pyrus_communis	pycom13g08190
rosa_chinensis	RchiOBHm_Chr4g0443641
rosa_laevigata	RLG00000005906
rosa_multiflora	Rmu_sc0001118.1_g000011 Rmu_sc0033917.1_g000002
rosa_roxburghii	Rroxscaffold_5G00384190
rosa_samantha	Rh4AG405800 Rh4BG416800 Rh4CG431300 Rh4DG412500
rosa_wichuraiana	Rw4G034840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	305, 408, 670
AclWI	GGATC	1 cut(s)	319
AcoI	YGGCCR	2 cut(s)	157, 302
AcsI	RAATTY	1 cut(s)	457
AcuI	CTGAAG	2 cut(s)	470, 753
AcvI	CACGTG	1 cut(s)	695
AfaI	GTAC	1 cut(s)	13
AfiI	CCNNNNNNNGG	1 cut(s)	629
AgsI	TTSAA	2 cut(s)	93, 455
AjnI	CCWGG	1 cut(s)	344
AluBI	AGCT	5 cut(s)	141, 191, 322, 636, 728
AluI	AGCT	5 cut(s)	141, 191, 322, 636, 728
AlwI	GGATC	1 cut(s)	319
AlwNI	CAGNNNCTG	1 cut(s)	731
AoxI	GGCC	6 cut(s)	57, 157, 278, 302, 619, 680
ApeKI	GCWGC	3 cut(s)	191, 590, 728
ApoI	RAATTY	1 cut(s)	457
AseI	ATTAAT	1 cut(s)	273
Asp700I	GAANNNNTTC	1 cut(s)	296
AspS9I	GGNCC	4 cut(s)	28, 150, 620, 680
AsuC2I	CCSGG	1 cut(s)	329
AsuHPI	GGTGA	2 cut(s)	164, 425
AvaII	GGWCC	2 cut(s)	28, 150
AxyI	CCTNAGG	1 cut(s)	624
BbrPI	CACGTG	1 cut(s)	695
BbsI	GAAGAC	1 cut(s)	497
BbvI	GCAGC	3 cut(s)	178, 602, 715
BccI	CCATC	4 cut(s)	71, 81, 431, 803
BceAI	ACGGC	1 cut(s)	172
BciT130I	CCWGG	1 cut(s)	346
BclI	TGATCA	1 cut(s)	415
BcnI	CCSGG	1 cut(s)	329
BfaI	CTAG	1 cut(s)	710
BglI	GCCNNNNNGGC	1 cut(s)	530
BisI	GCNGC	4 cut(s)	192, 305, 591, 729
BlsI	GCNGC	4 cut(s)	193, 306, 592, 730
Bme1390I	CCNGG	2 cut(s)	329, 346
Bme18I	GGWCC	2 cut(s)	28, 150
BmgT120I	GGNCC	4 cut(s)	28, 150, 620, 680
BmiI	GGNNCC	3 cut(s)	151, 152, 391
BmrFI	CCNGG	2 cut(s)	329, 346
BmrI	ACTGGG	1 cut(s)	390
BmsI	GCATC	2 cut(s)	331, 631
BmuI	ACTGGG	1 cut(s)	390
BpiI	GAAGAC	1 cut(s)	497
BpuMI	CCSGG	1 cut(s)	329
BsaAI	YACGTR	1 cut(s)	695
BsaJI	CCNNGG	6 cut(s)	129, 145, 154, 345, 393, 525
BsaWI	WCCGGW	1 cut(s)	769
BsaXI	ACNNNNNCTCC	2 cut(s)	543, 573
Bsc4I	CCNNNNNNNGG	1 cut(s)	629
Bse1I	ACTGG	3 cut(s)	385, 515, 622
Bse21I	CCTNAGG	1 cut(s)	624
Bse3DI	GCAATG	1 cut(s)	585
BseBI	CCWGG	1 cut(s)	346
BseDI	CCNNGG	6 cut(s)	129, 145, 154, 345, 393, 525
BseGI	GGATG	1 cut(s)	442
BseLI	CCNNNNNNNGG	1 cut(s)	629
BseMI	GCAATG	1 cut(s)	585
BseNI	ACTGG	3 cut(s)	385, 515, 622
BseX3I	CGGCCG	1 cut(s)	302
BseXI	GCAGC	3 cut(s)	178, 602, 715
Bsh1285I	CGRYCG	1 cut(s)	305
BshFI	GGCC	6 cut(s)	59, 159, 280, 304, 621, 682
BsiEI	CGRYCG	1 cut(s)	305
BsiSI	CCGG	5 cut(s)	207, 281, 301, 328, 770
BslFI	GGGAC	5 cut(s)	149, 163, 362, 652, 762
BslI	CCNNNNNNNGG	1 cut(s)	629
BsmFI	GGGAC	5 cut(s)	149, 163, 362, 652, 762
BsmI	GAATGC	1 cut(s)	298
BsnI	GGCC	6 cut(s)	59, 159, 280, 304, 621, 682
Bsp143I	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
Bsp19I	CCATGG	2 cut(s)	129, 525
BspACI	CCGC	3 cut(s)	305, 408, 670
BspANI	GGCC	6 cut(s)	59, 159, 280, 304, 621, 682
BspHI	TCATGA	1 cut(s)	33
BspLI	GGNNCC	3 cut(s)	151, 152, 391
BspPI	GGATC	1 cut(s)	319
BsrDI	GCAATG	1 cut(s)	585
BsrI	ACTGG	3 cut(s)	385, 515, 622
BssECI	CCNNGG	6 cut(s)	129, 145, 154, 345, 393, 525
BssMI	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
BssT1I	CCWWGG	3 cut(s)	129, 393, 525
Bst2UI	CCWGG	1 cut(s)	346
Bst4CI	ACNGT	2 cut(s)	356, 763
BstAPI	GCANNNNNTGC	2 cut(s)	562, 599
BstBAI	YACGTR	1 cut(s)	695
BstDEI	CTNAG	1 cut(s)	624
BstDSI	CCRYGG	4 cut(s)	129, 145, 154, 525
BstF5I	GGATG	1 cut(s)	442
BstKTI	GATC	7 cut(s)	87, 315, 327, 418, 580, 663, 834
BstMBI	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
BstMCI	CGRYCG	1 cut(s)	305
BstMWI	GCNNNNNNNGC	5 cut(s)	530, 539, 562, 599, 734
BstNI	CCWGG	1 cut(s)	346
BstNSI	RCATGY	1 cut(s)	560
BstSCI	CCNGG	2 cut(s)	327, 344
BstV1I	GCAGC	3 cut(s)	178, 602, 715
BstV2I	GAAGAC	1 cut(s)	497
BstZI	CGGCCG	1 cut(s)	302
Bsu36I	CCTNAGG	1 cut(s)	624
BsuRI	GGCC	6 cut(s)	59, 159, 280, 304, 621, 682
BtgI	CCRYGG	4 cut(s)	129, 145, 154, 525
BtsCI	GGATG	1 cut(s)	442
BtsIMutI	CAGTG	2 cut(s)	219, 759
CaiI	CAGNNNCTG	1 cut(s)	731
CciI	TCATGA	1 cut(s)	33
Cfr13I	GGNCC	4 cut(s)	28, 150, 620, 680
Csp6I	GTAC	1 cut(s)	12
CviAII	CATG	6 cut(s)	34, 130, 526, 557, 568, 664
CviQI	GTAC	1 cut(s)	12
DdeI	CTNAG	1 cut(s)	624
DpnI	GATC	7 cut(s)	86, 314, 326, 417, 579, 662, 833
DpnII	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
EaeI	YGGCCR	2 cut(s)	157, 302
EagI	CGGCCG	1 cut(s)	302
EclXI	CGGCCG	1 cut(s)	302
Eco130I	CCWWGG	3 cut(s)	129, 393, 525
Eco47I	GGWCC	2 cut(s)	28, 150
Eco52I	CGGCCG	1 cut(s)	302
Eco57I	CTGAAG	2 cut(s)	470, 753
Eco72I	CACGTG	1 cut(s)	695
Eco81I	CCTNAGG	1 cut(s)	624
EcoO109I	RGGNCCY	2 cut(s)	28, 150
EcoRII	CCWGG	1 cut(s)	344
EcoT14I	CCWWGG	3 cut(s)	129, 393, 525
ErhI	CCWWGG	3 cut(s)	129, 393, 525
FaeI	CATG	6 cut(s)	37, 133, 529, 560, 571, 667
FaqI	GGGAC	5 cut(s)	149, 163, 362, 652, 762
FatI	CATG	6 cut(s)	33, 129, 525, 556, 567, 663
FauI	CCCGC	1 cut(s)	677
FbaI	TGATCA	1 cut(s)	415
Fnu4HI	GCNGC	4 cut(s)	192, 305, 591, 729
FokI	GGATG	1 cut(s)	449
Fsp4HI	GCNGC	4 cut(s)	192, 305, 591, 729
FspBI	CTAG	1 cut(s)	710
GluI	GCNGC	4 cut(s)	192, 305, 591, 729
HaeIII	GGCC	6 cut(s)	59, 159, 280, 304, 621, 682
HapII	CCGG	5 cut(s)	207, 281, 301, 328, 770
Hin1II	CATG	6 cut(s)	37, 133, 529, 560, 571, 667
HincII	GTYRAC	2 cut(s)	199, 757
HindII	GTYRAC	2 cut(s)	199, 757
HindIII	AAGCTT	1 cut(s)	634
HinfI	GANTC	5 cut(s)	17, 79, 169, 203, 496
HpaI	GTTAAC	1 cut(s)	757
HpaII	CCGG	5 cut(s)	207, 281, 301, 328, 770
HphI	GGTGA	2 cut(s)	164, 425
Hpy166II	GTNNAC	4 cut(s)	46, 199, 219, 757
Hpy188I	TCNGA	6 cut(s)	22, 78, 84, 448, 495, 808
Hpy188III	TCNNGA	2 cut(s)	34, 166
Hpy8I	GTNNAC	4 cut(s)	46, 199, 219, 757
Hpy99I	CGWCG	2 cut(s)	248, 504
HpyAV	CCTTC	5 cut(s)	19, 245, 282, 379, 494
HpyCH4III	ACNGT	2 cut(s)	356, 763
HpyCH4IV	ACGT	1 cut(s)	694
HpyCH4V	TGCA	2 cut(s)	565, 590
HpyF10VI	GCNNNNNNNGC	5 cut(s)	530, 539, 562, 599, 734
HpyF3I	CTNAG	1 cut(s)	624
HpySE526I	ACGT	1 cut(s)	694
Hsp92II	CATG	6 cut(s)	37, 133, 529, 560, 571, 667
KflI	GGGWCCC	1 cut(s)	150
Ksp22I	TGATCA	1 cut(s)	415
KspAI	GTTAAC	1 cut(s)	757
Kzo9I	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
LmnI	GCTCC	1 cut(s)	319
Lsp1109I	GCAGC	3 cut(s)	178, 602, 715
LweI	GCATC	2 cut(s)	331, 631
MaeI	CTAG	1 cut(s)	710
MaeII	ACGT	1 cut(s)	694
MaeIII	GTNAC	1 cut(s)	789
MalI	GATC	7 cut(s)	86, 314, 326, 417, 579, 662, 833
MboI	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
MboII	GAAGA	3 cut(s)	63, 117, 497
MfeI	CAATTG	1 cut(s)	835
MluCI	AATT	5 cut(s)	88, 93, 457, 519, 835
MlyI	GAGTC	4 cut(s)	26, 73, 163, 212
MmeI	TCCRAC	2 cut(s)	93, 610
MnlI	CCTC	3 cut(s)	41, 70, 545
MroXI	GAANNNNTTC	1 cut(s)	296
MseI	TTAA	4 cut(s)	273, 336, 471, 756
MspA1I	CMGCKG	1 cut(s)	728
MspI	CCGG	5 cut(s)	207, 281, 301, 328, 770
MspR9I	CCNGG	2 cut(s)	329, 346
MunI	CAATTG	1 cut(s)	835
Mva1269I	GAATGC	1 cut(s)	298
MvaI	CCWGG	1 cut(s)	346
MwoI	GCNNNNNNNGC	5 cut(s)	530, 539, 562, 599, 734
NciI	CCSGG	1 cut(s)	329
NcoI	CCATGG	2 cut(s)	129, 525
NdeII	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
NlaIII	CATG	6 cut(s)	37, 133, 529, 560, 571, 667
NlaIV	GGNNCC	3 cut(s)	151, 152, 391
NspI	RCATGY	1 cut(s)	560
PagI	TCATGA	1 cut(s)	33
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PctI	GAATGC	1 cut(s)	298
PdmI	GAANNNNTTC	1 cut(s)	296
PfeI	GAWTC	1 cut(s)	496
PkrI	GCNGC	4 cut(s)	193, 306, 592, 730
PleI	GAGTC	4 cut(s)	25, 73, 163, 211
PmaCI	CACGTG	1 cut(s)	695
PmlI	CACGTG	1 cut(s)	695
PpsI	GAGTC	4 cut(s)	25, 73, 163, 211
Ppu21I	YACGTR	1 cut(s)	695
PpuMI	RGGWCCY	2 cut(s)	28, 150
PshBI	ATTAAT	1 cut(s)	273
Psp5II	RGGWCCY	2 cut(s)	28, 150
Psp6I	CCWGG	1 cut(s)	344
PspCI	CACGTG	1 cut(s)	695
PspGI	CCWGG	1 cut(s)	344
PspN4I	GGNNCC	3 cut(s)	151, 152, 391
PspPI	GGNCC	4 cut(s)	28, 150, 620, 680
PspPPI	RGGWCCY	2 cut(s)	28, 150
PstNI	CAGNNNCTG	1 cut(s)	731
PvuII	CAGCTG	1 cut(s)	728
RsaI	GTAC	1 cut(s)	13
RsaNI	GTAC	1 cut(s)	12
SaqAI	TTAA	4 cut(s)	273, 336, 471, 756
SatI	GCNGC	4 cut(s)	192, 305, 591, 729
Sau3AI	GATC	7 cut(s)	84, 312, 324, 415, 577, 660, 831
Sau96I	GGNCC	4 cut(s)	28, 150, 620, 680
SchI	GAGTC	4 cut(s)	26, 73, 163, 212
ScrFI	CCNGG	2 cut(s)	329, 346
SfaNI	GCATC	2 cut(s)	331, 631
SinI	GGWCC	2 cut(s)	28, 150
Sse9I	AATT	5 cut(s)	88, 93, 457, 519, 835
SsiI	CCGC	3 cut(s)	305, 408, 670
SspMI	CTAG	1 cut(s)	710
StyD4I	CCNGG	2 cut(s)	327, 344
StyI	CCWWGG	3 cut(s)	129, 393, 525
TaaI	ACNGT	2 cut(s)	356, 763
TaiI	ACGT	1 cut(s)	697
TaqI	TCGA	2 cut(s)	659, 830
TasI	AATT	5 cut(s)	88, 93, 457, 519, 835
TatI	WGTACW	1 cut(s)	11
TauI	GCSGC	1 cut(s)	307
TfiI	GAWTC	1 cut(s)	496
Tru1I	TTAA	4 cut(s)	273, 336, 471, 756
Tru9I	TTAA	4 cut(s)	273, 336, 471, 756
TscAI	CASTG	2 cut(s)	226, 766
TseI	GCWGC	3 cut(s)	191, 590, 728
TspDTI	ATGAA	2 cut(s)	50, 768
TspGWI	ACGGA	3 cut(s)	232, 449, 808
TspRI	CASTG	2 cut(s)	226, 766
VpaK11BI	GGWCC	2 cut(s)	28, 150
VspI	ATTAAT	1 cut(s)	273
XapI	RAATTY	1 cut(s)	457
XceI	RCATGY	1 cut(s)	560
XmnI	GAANNNNTTC	1 cut(s)	296
XspI	CTAG	1 cut(s)	710

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.