RchiOBHm_Chr4g0443641

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013538
Physical Location & Seq
Forward (+)
64719787 .. 64722334
2548 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41134

Sequence Viewer

Length: 1011 bp
ATGGCTCAAAGTACAGAGTCAGAGAAGGACCTCATGAACAGAGTGAACAGAAGAATGGCCTCCATCTCCAACCATCTGACTCCGATCAATTCAATTCCCAACTCCATTACTCTGTCGCACACATCTTCCATGGACGACAGCTACCACGGGACCCACGGCCAAGTCCCGACTCACCAAGTTGTCTGGGAAGCTGCTGTTGACGAGTCCGGCAAGGAGTTCACTGACATTATCTACGAGAAATCCGTCGGCGAAGGAATAGCAAAGATTACGATTAATAGGCCGGAGAGAAGGAATGCGTTCCGGCCGCAGACGATCAAGGAGCTGATCCGGGCTTTTAATGATGCCAGGGACGACAGTTCCATTGGCGTTATTGTTCTTACTGGGAAGGGAACCAAGGCATTTTGTAGCGGTGGTGATCAGGCTTTGAGAAGCACGGATGGTTATTCTGATTTTGAAAATTTTGGTCGTCTTAATGTTTTAGACCTTCAGGTTCAGATTCGTCGTCTTCCAAAGCCAGTAATTGCCATGGTGGCTGGTTATGCTGTTGGGGGAGGGCATGTGTTGCACATGGTATGTGATCTAACCATTGCAGCAGACAATGCTATTTTTGGTCAAACTGGCCCTAAGGTTGGAAGCTTTGATGCTGGTTATGGGAGTTCGATCATGTCCCGCTTGGTCGGGCCTAAAAAAGCACGTGAAATGTGGTTTCTAGCAAGGTTTTACACAGCTGCTGAAGCAGAGAAAATGGGACTTGTTAACACTGTTGTTCCGCTTGAGAATTTAGAGCAAGAAACGGTTAAATGGTGTCGAGAAATCCTAAGAAACAGCCCAACTGCAATTCGGGTGTTGAAATCATCTCTTAATGCAGTTGATGATGGTCATGCTGGACTCCAGGAACTTGGTGGAAATGCAACACTCATGTTCTATGGCACTGAGGAAGGAAATGAAGGGAAGACAGCATATATGCAACGTCGACGTCCAGATTTCACAAAGTTTCCCAGGCGACCTTAA

Protein Analysis

336

Amino Acids

37.03

Weight (kDa)

7.75

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 84 - 330 9.4e-74 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 87 - 263 1.5e-21 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60550
fragaria_vesca FvH4_4g34130 FvH4_4g34130 FvH4_4g34130
malus_domestica MD13G1094000.v1.1 MD16G1095300.v1.1
prunus_persica Prupe.1G251300_v2.0.a1
pyrus_communis pycom13g08190
rosa_chinensis RchiOBHm_Chr4g0443641
rosa_laevigata RLG00000005906
rosa_multiflora Rmu_sc0001118.1_g000011 Rmu_sc0033917.1_g000002
rosa_roxburghii Rroxscaffold_5G00384190
rosa_samantha Rh4AG405800 Rh4BG416800 Rh4CG431300 Rh4DG412500
rosa_wichuraiana Rw4G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 979
AccI GTMKAC 1 cut(s) 973
AciI CCGC 4 cut(s) 305, 408, 670, 770
AclWI GGATC 1 cut(s) 319
AcoI YGGCCR 2 cut(s) 157, 302
AcsI RAATTY 2 cut(s) 457, 778
AcuI CTGAAG 2 cut(s) 470, 753
AcvI CACGTG 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 976
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 629
AgsI TTSAA 3 cut(s) 93, 455, 850
AjnI CCWGG 3 cut(s) 344, 891, 998
AluBI AGCT 5 cut(s) 141, 191, 322, 636, 728
AluI AGCT 5 cut(s) 141, 191, 322, 636, 728
AlwI GGATC 1 cut(s) 319
AlwNI CAGNNNCTG 1 cut(s) 731
AoxI GGCC 6 cut(s) 57, 157, 278, 302, 619, 680
ApeKI GCWGC 3 cut(s) 191, 590, 728
ApoI RAATTY 2 cut(s) 457, 778
AseI ATTAAT 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 296
AspS9I GGNCC 4 cut(s) 28, 150, 620, 680
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 2 cut(s) 164, 425
AvaII GGWCC 2 cut(s) 28, 150
AxyI CCTNAGG 1 cut(s) 624
BbrPI CACGTG 1 cut(s) 695
BbsI GAAGAC 2 cut(s) 497, 959
BbvI GCAGC 3 cut(s) 178, 602, 715
BccI CCATC 4 cut(s) 71, 81, 431, 869
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 3 cut(s) 346, 893, 1000
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 329
BfaI CTAG 1 cut(s) 710
BglI GCCNNNNNGGC 1 cut(s) 530
BisI GCNGC 4 cut(s) 192, 305, 591, 729
BlsI GCNGC 4 cut(s) 193, 306, 592, 730
Bme1390I CCNGG 4 cut(s) 329, 346, 893, 1000
Bme18I GGWCC 2 cut(s) 28, 150
BmgT120I GGNCC 4 cut(s) 28, 150, 620, 680
BmiI GGNNCC 3 cut(s) 151, 152, 391
BmrFI CCNGG 4 cut(s) 329, 346, 893, 1000
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 2 cut(s) 331, 631
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 497, 959
BpmI CTGGAG 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 329
BsaAI YACGTR 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 976
BsaJI CCNNGG 7 cut(s) 129, 145, 154, 345, 393, 525, 998
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 629
Bse1I ACTGG 3 cut(s) 385, 515, 622
Bse21I CCTNAGG 1 cut(s) 624
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 3 cut(s) 346, 893, 1000
BseDI CCNNGG 7 cut(s) 129, 145, 154, 345, 393, 525, 998
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 1 cut(s) 629
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 1 cut(s) 924
BseNI ACTGG 3 cut(s) 385, 515, 622
BseX3I CGGCCG 1 cut(s) 302
BseXI GCAGC 3 cut(s) 178, 602, 715
Bsh1285I CGRYCG 1 cut(s) 305
BshFI GGCC 6 cut(s) 59, 159, 280, 304, 621, 682
BsiEI CGRYCG 1 cut(s) 305
BsiSI CCGG 4 cut(s) 207, 281, 301, 328
BslFI GGGAC 5 cut(s) 149, 163, 362, 652, 762
BslI CCNNNNNNNGG 1 cut(s) 629
BsmFI GGGAC 5 cut(s) 149, 163, 362, 652, 762
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 6 cut(s) 59, 159, 280, 304, 621, 682
Bsp143I GATC 6 cut(s) 84, 312, 324, 415, 577, 660
Bsp19I CCATGG 2 cut(s) 129, 525
BspACI CCGC 4 cut(s) 305, 408, 670, 770
BspANI GGCC 6 cut(s) 59, 159, 280, 304, 621, 682
BspCNI CTCAG 1 cut(s) 925
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 3 cut(s) 151, 152, 391
BspPI GGATC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 585
BsrI ACTGG 3 cut(s) 385, 515, 622
BssECI CCNNGG 7 cut(s) 129, 145, 154, 345, 393, 525, 998
BssMI GATC 6 cut(s) 84, 312, 324, 415, 577, 660
BssNI GRCGYC 1 cut(s) 976
BssT1I CCWWGG 3 cut(s) 129, 393, 525
Bst2UI CCWGG 3 cut(s) 346, 893, 1000
Bst4CI ACNGT 3 cut(s) 356, 763, 796
BstACI GRCGYC 1 cut(s) 976
BstAPI GCANNNNNTGC 2 cut(s) 562, 599
BstBAI YACGTR 1 cut(s) 695
BstDEI CTNAG 3 cut(s) 624, 818, 933
BstDSI CCRYGG 4 cut(s) 129, 145, 154, 525
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 6 cut(s) 87, 315, 327, 418, 580, 663
BstMBI GATC 6 cut(s) 84, 312, 324, 415, 577, 660
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 5 cut(s) 530, 539, 562, 599, 734
BstNI CCWGG 3 cut(s) 346, 893, 1000
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 4 cut(s) 327, 344, 891, 998
BstV1I GCAGC 3 cut(s) 178, 602, 715
BstV2I GAAGAC 2 cut(s) 497, 959
BstXI CCANNNNNNTGG 1 cut(s) 899
BstZI CGGCCG 1 cut(s) 302
Bsu36I CCTNAGG 1 cut(s) 624
BsuRI GGCC 6 cut(s) 59, 159, 280, 304, 621, 682
BtgI CCRYGG 4 cut(s) 129, 145, 154, 525
BtsCI GGATG 1 cut(s) 442
BtsIMutI CAGTG 3 cut(s) 219, 759, 930
CaiI CAGNNNCTG 1 cut(s) 731
CciI TCATGA 1 cut(s) 33
Cfr13I GGNCC 4 cut(s) 28, 150, 620, 680
Csp6I GTAC 1 cut(s) 12
CviAII CATG 8 cut(s) 34, 130, 526, 557, 568, 664, 881, 919
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 3 cut(s) 624, 818, 933
DpnI GATC 6 cut(s) 86, 314, 326, 417, 579, 662
DpnII GATC 6 cut(s) 84, 312, 324, 415, 577, 660
EaeI YGGCCR 2 cut(s) 157, 302
EagI CGGCCG 1 cut(s) 302
EclXI CGGCCG 1 cut(s) 302
Eco130I CCWWGG 3 cut(s) 129, 393, 525
Eco47I GGWCC 2 cut(s) 28, 150
Eco52I CGGCCG 1 cut(s) 302
Eco57I CTGAAG 2 cut(s) 470, 753
Eco72I CACGTG 1 cut(s) 695
Eco81I CCTNAGG 1 cut(s) 624
EcoO109I RGGNCCY 2 cut(s) 28, 150
EcoRII CCWGG 3 cut(s) 344, 891, 998
EcoT14I CCWWGG 3 cut(s) 129, 393, 525
ErhI CCWWGG 3 cut(s) 129, 393, 525
FaeI CATG 8 cut(s) 37, 133, 529, 560, 571, 667, 884, 922
FaqI GGGAC 5 cut(s) 149, 163, 362, 652, 762
FatI CATG 8 cut(s) 33, 129, 525, 556, 567, 663, 880, 918
FauI CCCGC 1 cut(s) 677
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 973
Fnu4HI GCNGC 4 cut(s) 192, 305, 591, 729
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 4 cut(s) 192, 305, 591, 729
FspBI CTAG 1 cut(s) 710
GluI GCNGC 4 cut(s) 192, 305, 591, 729
GsuI CTGGAG 1 cut(s) 875
HaeIII GGCC 6 cut(s) 59, 159, 280, 304, 621, 682
HapII CCGG 4 cut(s) 207, 281, 301, 328
Hin1I GRCGYC 1 cut(s) 976
Hin1II CATG 8 cut(s) 37, 133, 529, 560, 571, 667, 884, 922
HincII GTYRAC 3 cut(s) 199, 757, 974
HindII GTYRAC 3 cut(s) 199, 757, 974
HindIII AAGCTT 1 cut(s) 634
HinfI GANTC 6 cut(s) 17, 79, 169, 203, 496, 888
HpaI GTTAAC 1 cut(s) 757
HpaII CCGG 4 cut(s) 207, 281, 301, 328
HphI GGTGA 2 cut(s) 164, 425
Hpy166II GTNNAC 5 cut(s) 46, 199, 219, 757, 974
Hpy188I TCNGA 5 cut(s) 22, 78, 84, 448, 495
Hpy188III TCNNGA 4 cut(s) 34, 166, 809, 980
Hpy8I GTNNAC 5 cut(s) 46, 199, 219, 757, 974
Hpy99I CGWCG 4 cut(s) 248, 504, 975, 978
HpyAV CCTTC 7 cut(s) 19, 245, 282, 379, 494, 932, 941
HpyCH4III ACNGT 3 cut(s) 356, 763, 796
HpyCH4IV ACGT 3 cut(s) 694, 970, 976
HpyCH4V TGCA 6 cut(s) 565, 590, 836, 866, 911, 967
HpyF10VI GCNNNNNNNGC 5 cut(s) 530, 539, 562, 599, 734
HpyF3I CTNAG 3 cut(s) 624, 818, 933
HpySE526I ACGT 3 cut(s) 694, 970, 976
Hsp92I GRCGYC 1 cut(s) 976
Hsp92II CATG 8 cut(s) 37, 133, 529, 560, 571, 667, 884, 922
KflI GGGWCCC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 415
KspAI GTTAAC 1 cut(s) 757
Kzo9I GATC 6 cut(s) 84, 312, 324, 415, 577, 660
LmnI GCTCC 1 cut(s) 319
Lsp1109I GCAGC 3 cut(s) 178, 602, 715
LweI GCATC 2 cut(s) 331, 631
MaeI CTAG 1 cut(s) 710
MaeII ACGT 3 cut(s) 694, 970, 976
MalI GATC 6 cut(s) 86, 314, 326, 417, 579, 662
MboI GATC 6 cut(s) 84, 312, 324, 415, 577, 660
MboII GAAGA 4 cut(s) 63, 117, 497, 964
MluCI AATT 6 cut(s) 88, 93, 457, 519, 778, 837
MlyI GAGTC 5 cut(s) 26, 73, 163, 212, 882
MmeI TCCRAC 2 cut(s) 93, 610
MnlI CCTC 4 cut(s) 41, 70, 545, 928
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 7 cut(s) 273, 336, 471, 756, 798, 861, 1009
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 4 cut(s) 207, 281, 301, 328
MspR9I CCNGG 4 cut(s) 329, 346, 893, 1000
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 3 cut(s) 346, 893, 1000
MwoI GCNNNNNNNGC 5 cut(s) 530, 539, 562, 599, 734
NciI CCSGG 1 cut(s) 329
NcoI CCATGG 2 cut(s) 129, 525
NdeII GATC 6 cut(s) 84, 312, 324, 415, 577, 660
NlaIII CATG 8 cut(s) 37, 133, 529, 560, 571, 667, 884, 922
NlaIV GGNNCC 3 cut(s) 151, 152, 391
NspI RCATGY 1 cut(s) 560
PagI TCATGA 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 496
PfoI TCCNGGA 1 cut(s) 891
PkrI GCNGC 4 cut(s) 193, 306, 592, 730
PleI GAGTC 5 cut(s) 25, 73, 163, 211, 882
PmaCI CACGTG 1 cut(s) 695
PmlI CACGTG 1 cut(s) 695
PpsI GAGTC 5 cut(s) 25, 73, 163, 211, 882
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 2 cut(s) 28, 150
PshBI ATTAAT 1 cut(s) 273
Psp5II RGGWCCY 2 cut(s) 28, 150
Psp6I CCWGG 3 cut(s) 344, 891, 998
PspCI CACGTG 1 cut(s) 695
PspGI CCWGG 3 cut(s) 344, 891, 998
PspN4I GGNNCC 3 cut(s) 151, 152, 391
PspPI GGNCC 4 cut(s) 28, 150, 620, 680
PspPPI RGGWCCY 2 cut(s) 28, 150
PstNI CAGNNNCTG 1 cut(s) 731
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SalI GTCGAC 1 cut(s) 972
SaqAI TTAA 7 cut(s) 273, 336, 471, 756, 798, 861, 1009
SatI GCNGC 4 cut(s) 192, 305, 591, 729
Sau3AI GATC 6 cut(s) 84, 312, 324, 415, 577, 660
Sau96I GGNCC 4 cut(s) 28, 150, 620, 680
SchI GAGTC 5 cut(s) 26, 73, 163, 212, 882
ScrFI CCNGG 4 cut(s) 329, 346, 893, 1000
SfaNI GCATC 2 cut(s) 331, 631
SgrDI CGTCGACG 1 cut(s) 972
SinI GGWCC 2 cut(s) 28, 150
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
Sse9I AATT 6 cut(s) 88, 93, 457, 519, 778, 837
SsiI CCGC 4 cut(s) 305, 408, 670, 770
SspMI CTAG 1 cut(s) 710
StyD4I CCNGG 4 cut(s) 327, 344, 891, 998
StyI CCWWGG 3 cut(s) 129, 393, 525
TaaI ACNGT 3 cut(s) 356, 763, 796
TaiI ACGT 3 cut(s) 697, 973, 979
TaqI TCGA 3 cut(s) 659, 808, 973
TasI AATT 6 cut(s) 88, 93, 457, 519, 778, 837
TatI WGTACW 1 cut(s) 11
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 1 cut(s) 496
Tru1I TTAA 7 cut(s) 273, 336, 471, 756, 798, 861, 1009
Tru9I TTAA 7 cut(s) 273, 336, 471, 756, 798, 861, 1009
TscAI CASTG 3 cut(s) 226, 766, 937
TseI GCWGC 3 cut(s) 191, 590, 728
TspDTI ATGAA 2 cut(s) 50, 960
TspGWI ACGGA 2 cut(s) 232, 449
TspRI CASTG 3 cut(s) 226, 766, 937
VpaK11BI GGWCC 2 cut(s) 28, 150
VspI ATTAAT 1 cut(s) 273
XapI RAATTY 2 cut(s) 457, 778
XceI RCATGY 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 973
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 1 cut(s) 710
ZraI GACGTC 1 cut(s) 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.