RLG00000005906

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013538
Physical Location & Seq
Reverse (-)
2683334 .. 2685281
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005906

Sequence Viewer

Length: 1005 bp
ATGGCTCAAAGTACAGAGAAGGACCTCATGAACAGAGTGAACAGAAGAATGGCCTCCATCTCCAACCATCTGACTCCGATCAATTCAATTCCCAACTCCATTACTCTGTCGCACACATCTTCCATGGACGACAGCTACCACCGGACCCACGGCCAAGTCCCGACTCACCAAGTTGTCTGGGAAGCTGCTGTTGACGAGTCCGGCAAGGAGTTCACTGACATTATCTACGAGAAATCCGTCGGCGAAGGAATAGCAAAGATTACGATTAATAGGCCGGAGAGAAGGAATGCGTTCCGGCCGCAGACGATTAAGGAGCTGATGCGGGCTTTTAATGATGCCAGGGACGATAGTTCCATTGGCGTTATTGTTCTTACTGGGAAGGGAACCAAGGCATTTTGTAGCGGTGGTGATCAGGCTTTGAGAAGCACGGATGGTTATTCTGATTTTGAAAATTTTGGTCGTCTTAATGTTTTAGACCTTCAGGTTCAGATTCGTCGTCTTCCAAAGCCAGTAATTGCCATGGTGGCTGGTTATGCTGTTGGGGGAGGGCATGTGTTGCACATGGTATGTGATCTAACCATTGCAGCAGACAATGCTATTTTTGGTCAAACTGGCCCTAAGGTTGGAAGCTTTGATGCTGGTTATGGGAGTTCGATCATGTCCCGCTTGGTCGGGCCTAAAAAAGCACGTGAAATGTGGTTTCTAGCAAGGTTTTACACAGCTGCTGAAGCAGAGAAAATGGGACTTGTTAACACTGTTATACCGCTTGAGAATTTAGAGCAAGAAACGGTTAAATGGTGTCGAGAAATCCTAAGAAACAGCCCAACTGCAATTCGGGTGTTGAAATCATCTCTTAATGCAGTTGATGATGGTCATGCTGGACTCCAGGAACTTGGTGGAAATGCAACACTCATATTCTATGGCACTGAGGAAGGAAATGAAGGGAAGACAGCATATATGCAACGTCGACGTCCAGATTTCACAAAGTTTCCCAGGCGACCTTAA

Protein Analysis

335

Amino Acids

36.92

Weight (kDa)

8.67

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 82 - 328 6.6e-73 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 85 - 261 1.3e-21 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60550
fragaria_vesca FvH4_4g34130 FvH4_4g34130 FvH4_4g34130
malus_domestica MD13G1094000.v1.1 MD16G1095300.v1.1
prunus_persica Prupe.1G251300_v2.0.a1
pyrus_communis pycom13g08190
rosa_chinensis RchiOBHm_Chr4g0443641
rosa_laevigata RLG00000005906
rosa_multiflora Rmu_sc0001118.1_g000011 Rmu_sc0033917.1_g000002
rosa_roxburghii Rroxscaffold_5G00384190
rosa_samantha Rh4AG405800 Rh4BG416800 Rh4CG431300 Rh4DG412500
rosa_wichuraiana Rw4G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 973
AccI GTMKAC 1 cut(s) 967
AciI CCGC 5 cut(s) 299, 322, 402, 664, 764
AcoI YGGCCR 2 cut(s) 151, 296
AcsI RAATTY 2 cut(s) 451, 772
AcuI CTGAAG 2 cut(s) 464, 747
AcvI CACGTG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 970
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 623
AgsI TTSAA 3 cut(s) 87, 449, 844
AjnI CCWGG 3 cut(s) 338, 885, 992
AluBI AGCT 5 cut(s) 135, 185, 316, 630, 722
AluI AGCT 5 cut(s) 135, 185, 316, 630, 722
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 6 cut(s) 51, 151, 272, 296, 613, 674
ApeKI GCWGC 3 cut(s) 185, 584, 722
ApoI RAATTY 2 cut(s) 451, 772
AseI ATTAAT 1 cut(s) 267
Asp700I GAANNNNTTC 1 cut(s) 290
AspS9I GGNCC 4 cut(s) 22, 144, 614, 674
AsuHPI GGTGA 2 cut(s) 158, 419
AvaII GGWCC 2 cut(s) 22, 144
AxyI CCTNAGG 1 cut(s) 618
BbrPI CACGTG 1 cut(s) 689
BbsI GAAGAC 2 cut(s) 491, 953
BbvI GCAGC 3 cut(s) 172, 596, 709
BccI CCATC 4 cut(s) 65, 75, 425, 863
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 3 cut(s) 340, 887, 994
BclI TGATCA 1 cut(s) 409
BfaI CTAG 1 cut(s) 704
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 4 cut(s) 186, 299, 585, 723
BlsI GCNGC 4 cut(s) 187, 300, 586, 724
Bme1390I CCNGG 3 cut(s) 340, 887, 994
Bme18I GGWCC 2 cut(s) 22, 144
BmgT120I GGNCC 4 cut(s) 22, 144, 614, 674
BmiI GGNNCC 2 cut(s) 146, 385
BmrFI CCNGG 3 cut(s) 340, 887, 994
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 3 cut(s) 309, 325, 625
BmuI ACTGGG 1 cut(s) 384
BpiI GAAGAC 2 cut(s) 491, 953
BpmI CTGGAG 1 cut(s) 869
BpuEI CTTGAG 1 cut(s) 788
BsaAI YACGTR 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 970
BsaJI CCNNGG 6 cut(s) 123, 148, 339, 387, 519, 992
BsaWI WCCGGW 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse1I ACTGG 3 cut(s) 379, 509, 616
Bse21I CCTNAGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 3 cut(s) 340, 887, 994
BseDI CCNNGG 6 cut(s) 123, 148, 339, 387, 519, 992
BseGI GGATG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 1 cut(s) 918
BseNI ACTGG 3 cut(s) 379, 509, 616
BseX3I CGGCCG 1 cut(s) 296
BseXI GCAGC 3 cut(s) 172, 596, 709
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 6 cut(s) 53, 153, 274, 298, 615, 676
BsiEI CGRYCG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 142, 201, 275, 295
BslFI GGGAC 4 cut(s) 143, 356, 646, 756
BslI CCNNNNNNNGG 1 cut(s) 623
BsmFI GGGAC 4 cut(s) 143, 356, 646, 756
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 6 cut(s) 53, 153, 274, 298, 615, 676
Bsp143I GATC 4 cut(s) 78, 409, 571, 654
Bsp19I CCATGG 2 cut(s) 123, 519
BspACI CCGC 5 cut(s) 299, 322, 402, 664, 764
BspANI GGCC 6 cut(s) 53, 153, 274, 298, 615, 676
BspCNI CTCAG 1 cut(s) 919
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 146, 385
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 3 cut(s) 379, 509, 616
BssECI CCNNGG 6 cut(s) 123, 148, 339, 387, 519, 992
BssMI GATC 4 cut(s) 78, 409, 571, 654
BssNI GRCGYC 1 cut(s) 970
BssT1I CCWWGG 3 cut(s) 123, 387, 519
Bst2UI CCWGG 3 cut(s) 340, 887, 994
Bst4CI ACNGT 2 cut(s) 757, 790
BstACI GRCGYC 1 cut(s) 970
BstAPI GCANNNNNTGC 2 cut(s) 556, 593
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 3 cut(s) 618, 812, 927
BstDSI CCRYGG 3 cut(s) 123, 148, 519
BstF5I GGATG 1 cut(s) 436
BstKTI GATC 4 cut(s) 81, 412, 574, 657
BstMBI GATC 4 cut(s) 78, 409, 571, 654
BstMCI CGRYCG 1 cut(s) 299
BstMWI GCNNNNNNNGC 5 cut(s) 524, 533, 556, 593, 728
BstNI CCWGG 3 cut(s) 340, 887, 994
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 3 cut(s) 338, 885, 992
BstV1I GCAGC 3 cut(s) 172, 596, 709
BstV2I GAAGAC 2 cut(s) 491, 953
BstXI CCANNNNNNTGG 1 cut(s) 893
BstZI CGGCCG 1 cut(s) 296
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 6 cut(s) 53, 153, 274, 298, 615, 676
BtgI CCRYGG 3 cut(s) 123, 148, 519
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 213, 753, 924
Cac8I GCNNGC 1 cut(s) 324
CaiI CAGNNNCTG 1 cut(s) 725
CciI TCATGA 1 cut(s) 27
Cfr13I GGNCC 4 cut(s) 22, 144, 614, 674
Csp6I GTAC 1 cut(s) 12
CviAII CATG 7 cut(s) 28, 124, 520, 551, 562, 658, 875
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 3 cut(s) 618, 812, 927
DpnI GATC 4 cut(s) 80, 411, 573, 656
DpnII GATC 4 cut(s) 78, 409, 571, 654
EaeI YGGCCR 2 cut(s) 151, 296
EagI CGGCCG 1 cut(s) 296
EclXI CGGCCG 1 cut(s) 296
Eco130I CCWWGG 3 cut(s) 123, 387, 519
Eco47I GGWCC 2 cut(s) 22, 144
Eco52I CGGCCG 1 cut(s) 296
Eco57I CTGAAG 2 cut(s) 464, 747
Eco72I CACGTG 1 cut(s) 689
Eco81I CCTNAGG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 22
EcoRII CCWGG 3 cut(s) 338, 885, 992
EcoT14I CCWWGG 3 cut(s) 123, 387, 519
ErhI CCWWGG 3 cut(s) 123, 387, 519
FaeI CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
FaqI GGGAC 4 cut(s) 143, 356, 646, 756
FatI CATG 7 cut(s) 27, 123, 519, 550, 561, 657, 874
FauI CCCGC 2 cut(s) 315, 671
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 4 cut(s) 186, 299, 585, 723
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 4 cut(s) 186, 299, 585, 723
FspBI CTAG 1 cut(s) 704
GluI GCNGC 4 cut(s) 186, 299, 585, 723
GsuI CTGGAG 1 cut(s) 869
HaeIII GGCC 6 cut(s) 53, 153, 274, 298, 615, 676
HapII CCGG 4 cut(s) 142, 201, 275, 295
Hin1I GRCGYC 1 cut(s) 970
Hin1II CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
HincII GTYRAC 3 cut(s) 193, 751, 968
HindII GTYRAC 3 cut(s) 193, 751, 968
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 5 cut(s) 73, 163, 197, 490, 882
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 4 cut(s) 142, 201, 275, 295
HphI GGTGA 2 cut(s) 158, 419
Hpy166II GTNNAC 5 cut(s) 40, 193, 213, 751, 968
Hpy188I TCNGA 4 cut(s) 72, 78, 442, 489
Hpy188III TCNNGA 4 cut(s) 28, 160, 803, 974
Hpy8I GTNNAC 5 cut(s) 40, 193, 213, 751, 968
Hpy99I CGWCG 4 cut(s) 242, 498, 969, 972
HpyAV CCTTC 7 cut(s) 13, 239, 276, 373, 488, 926, 935
HpyCH4III ACNGT 2 cut(s) 757, 790
HpyCH4IV ACGT 3 cut(s) 688, 964, 970
HpyCH4V TGCA 6 cut(s) 559, 584, 830, 860, 905, 961
HpyF10VI GCNNNNNNNGC 5 cut(s) 524, 533, 556, 593, 728
HpyF3I CTNAG 3 cut(s) 618, 812, 927
HpySE526I ACGT 3 cut(s) 688, 964, 970
Hsp92I GRCGYC 1 cut(s) 970
Hsp92II CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
Ksp22I TGATCA 1 cut(s) 409
KspAI GTTAAC 1 cut(s) 751
Kzo9I GATC 4 cut(s) 78, 409, 571, 654
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 3 cut(s) 172, 596, 709
LweI GCATC 3 cut(s) 309, 325, 625
MaeI CTAG 1 cut(s) 704
MaeII ACGT 3 cut(s) 688, 964, 970
MalI GATC 4 cut(s) 80, 411, 573, 656
MboI GATC 4 cut(s) 78, 409, 571, 654
MboII GAAGA 4 cut(s) 57, 111, 491, 958
MluCI AATT 6 cut(s) 82, 87, 451, 513, 772, 831
MlyI GAGTC 4 cut(s) 67, 157, 206, 876
MmeI TCCRAC 2 cut(s) 87, 604
MnlI CCTC 4 cut(s) 35, 64, 539, 922
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 4 cut(s) 142, 201, 275, 295
MspR9I CCNGG 3 cut(s) 340, 887, 994
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 3 cut(s) 340, 887, 994
MwoI GCNNNNNNNGC 5 cut(s) 524, 533, 556, 593, 728
NcoI CCATGG 2 cut(s) 123, 519
NdeII GATC 4 cut(s) 78, 409, 571, 654
NlaIII CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
NlaIV GGNNCC 2 cut(s) 146, 385
NspI RCATGY 1 cut(s) 554
PagI TCATGA 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 490
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 4 cut(s) 187, 300, 586, 724
PleI GAGTC 4 cut(s) 67, 157, 205, 876
PmaCI CACGTG 1 cut(s) 689
PmlI CACGTG 1 cut(s) 689
PpsI GAGTC 4 cut(s) 67, 157, 205, 876
Ppu21I YACGTR 1 cut(s) 689
PpuMI RGGWCCY 1 cut(s) 22
PshBI ATTAAT 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 3 cut(s) 338, 885, 992
PspCI CACGTG 1 cut(s) 689
PspGI CCWGG 3 cut(s) 338, 885, 992
PspN4I GGNNCC 2 cut(s) 146, 385
PspPI GGNCC 4 cut(s) 22, 144, 614, 674
PspPPI RGGWCCY 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 725
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SalI GTCGAC 1 cut(s) 966
SaqAI TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
SatI GCNGC 4 cut(s) 186, 299, 585, 723
Sau3AI GATC 4 cut(s) 78, 409, 571, 654
Sau96I GGNCC 4 cut(s) 22, 144, 614, 674
SchI GAGTC 4 cut(s) 67, 157, 206, 876
ScrFI CCNGG 3 cut(s) 340, 887, 994
SfaNI GCATC 3 cut(s) 309, 325, 625
SgrDI CGTCGACG 1 cut(s) 966
SinI GGWCC 2 cut(s) 22, 144
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 6 cut(s) 82, 87, 451, 513, 772, 831
SsiI CCGC 5 cut(s) 299, 322, 402, 664, 764
SspMI CTAG 1 cut(s) 704
StyD4I CCNGG 3 cut(s) 338, 885, 992
StyI CCWWGG 3 cut(s) 123, 387, 519
TaaI ACNGT 2 cut(s) 757, 790
TaiI ACGT 3 cut(s) 691, 967, 973
TaqI TCGA 3 cut(s) 653, 802, 967
TasI AATT 6 cut(s) 82, 87, 451, 513, 772, 831
TatI WGTACW 1 cut(s) 11
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 1 cut(s) 490
Tru1I TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
Tru9I TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
TscAI CASTG 3 cut(s) 220, 760, 931
TseI GCWGC 3 cut(s) 185, 584, 722
TspDTI ATGAA 2 cut(s) 44, 954
TspGWI ACGGA 2 cut(s) 226, 443
TspRI CASTG 3 cut(s) 220, 760, 931
VpaK11BI GGWCC 2 cut(s) 22, 144
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 2 cut(s) 451, 772
XceI RCATGY 1 cut(s) 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 967
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 1 cut(s) 704
ZraI GACGTC 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.