Rroxscaffold_5G00384190

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013538
Physical Location & Seq
Forward (+)
63690873 .. 63696645
5773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384190.1

Sequence Viewer

Length: 1005 bp
ATGGCTCAAAGTACAGAGAAGGACCTCATGAACAGAGTGAACAGAAGAATCGCCTCCATCTCCAACCATCTCACTCCGATCAATTCAATTCCCAACTCCATTACTCTGTCGCACACATCTTCCATGGATGACAGCTACCACCGGACCCACGGCCAAGTCCCGACTCACCAAGTTGTCTGGGAAGCTGCCGTTGACGAGTCCAGCAAGGAGTTCACTGACATTATCTACGAGAAATCCGTCGGCGAAGGAATAGCAAAGATTACGATTAATAGGCCGGAGAGAAGGAATGCGTTCCGGCCGCAGACGATTAAGGAGCTGATCCGGGCTTTTAATGATGCCAGGGACGATAGTTCCATTGGCGTTATTGTTCTTACTGGGAAGGGAACCAAGGCATTTTGTAGCGGTGGTGATCAGGCTTTGAGAAGCACGGATGGTTATTCTGATTTTGAAAATTTTGGTCGTCTTAATGTTTTAGACCTTCAGGTTCAGATTCGTCGTCTTCCAAAGCCAGTAATTGCCATGGTGGCTGGTTATGCTGTTGGGGGAGGGCATGTGTTGCACATGGTATGTGATCTAACCATTGCAGCAGACAATGCTATTTTTGGTCAAACTGGCCCTAAGGTTGGAAGCTTTGATGCTGGTTATGGGAGTTCGATCATGTCCCGCTTGGTCGGGCCTAAAAAAGCACGTGAAATGTGGTTTCTAGCAAGGTTTTACACAGCTGCTGAAGCAGAGAAAATGGGACTTGTTAACACTGTTGTGCCGCTTGAGAATTTAGAGCAAGAAACGGTTAAATGGTGTCGAGAAATCCTAAGAAACAGCCCAACTGCAATTCGGGTGTTGAAATCATCTCTTAATGCAGTTGATGATGGTCATGCTGGACTCCAGGAACTTGGTGGAAATGCAACACTCATATTCTATGGCACCGAGGAAGGAAATGAAGGGAAGACATCATATATGCAACGTCGACGTCCAGATTTCACAAAGTTTCCCAGGCGACCTTAA

Protein Analysis

334

Amino Acids

36.92

Weight (kDa)

8.67

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 82 - 328 2.4e-73 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 85 - 261 1.5e-21 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60550
fragaria_vesca FvH4_4g34130 FvH4_4g34130 FvH4_4g34130
malus_domestica MD13G1094000.v1.1 MD16G1095300.v1.1
prunus_persica Prupe.1G251300_v2.0.a1
pyrus_communis pycom13g08190
rosa_chinensis RchiOBHm_Chr4g0443641
rosa_laevigata RLG00000005906
rosa_multiflora Rmu_sc0001118.1_g000011 Rmu_sc0033917.1_g000002
rosa_roxburghii Rroxscaffold_5G00384190
rosa_samantha Rh4AG405800 Rh4BG416800 Rh4CG431300 Rh4DG412500
rosa_wichuraiana Rw4G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 973
AccB1I GGYRCC 1 cut(s) 923
AccI GTMKAC 1 cut(s) 967
AciI CCGC 4 cut(s) 299, 402, 664, 764
AclWI GGATC 1 cut(s) 313
AcoI YGGCCR 2 cut(s) 151, 296
AcsI RAATTY 2 cut(s) 451, 772
AcuI CTGAAG 2 cut(s) 464, 747
AcvI CACGTG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 970
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 623
AgsI TTSAA 3 cut(s) 87, 449, 844
AjnI CCWGG 3 cut(s) 338, 885, 992
AleI CACNNNNGTG 1 cut(s) 758
AluBI AGCT 5 cut(s) 135, 185, 316, 630, 722
AluI AGCT 5 cut(s) 135, 185, 316, 630, 722
AlwI GGATC 1 cut(s) 313
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 5 cut(s) 151, 272, 296, 613, 674
ApeKI GCWGC 3 cut(s) 185, 584, 722
ApoI RAATTY 2 cut(s) 451, 772
AseI ATTAAT 1 cut(s) 267
Asp700I GAANNNNTTC 1 cut(s) 290
AspS9I GGNCC 4 cut(s) 22, 144, 614, 674
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 158, 419
AvaII GGWCC 2 cut(s) 22, 144
AxyI CCTNAGG 1 cut(s) 618
BanI GGYRCC 1 cut(s) 923
BbrPI CACGTG 1 cut(s) 689
BbsI GAAGAC 2 cut(s) 491, 953
BbvI GCAGC 3 cut(s) 172, 596, 709
BccI CCATC 4 cut(s) 65, 75, 425, 863
BceAI ACGGC 2 cut(s) 166, 173
BciT130I CCWGG 3 cut(s) 340, 887, 994
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 323
BfaI CTAG 1 cut(s) 704
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 5 cut(s) 186, 299, 585, 723, 764
BlsI GCNGC 5 cut(s) 187, 300, 586, 724, 765
Bme1390I CCNGG 4 cut(s) 323, 340, 887, 994
Bme18I GGWCC 2 cut(s) 22, 144
BmgT120I GGNCC 4 cut(s) 22, 144, 614, 674
BmiI GGNNCC 3 cut(s) 146, 385, 925
BmrFI CCNGG 4 cut(s) 323, 340, 887, 994
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 2 cut(s) 325, 625
BmuI ACTGGG 1 cut(s) 384
BpiI GAAGAC 2 cut(s) 491, 953
BpmI CTGGAG 1 cut(s) 869
BpuEI CTTGAG 1 cut(s) 788
BpuMI CCSGG 1 cut(s) 323
BsaAI YACGTR 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 970
BsaJI CCNNGG 7 cut(s) 123, 148, 339, 387, 519, 927, 992
BsaWI WCCGGW 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse1I ACTGG 3 cut(s) 379, 509, 616
Bse21I CCTNAGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 3 cut(s) 340, 887, 994
BseDI CCNNGG 7 cut(s) 123, 148, 339, 387, 519, 927, 992
BseGI GGATG 2 cut(s) 133, 436
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 1 cut(s) 579
BseNI ACTGG 3 cut(s) 379, 509, 616
BseX3I CGGCCG 1 cut(s) 296
BseXI GCAGC 3 cut(s) 172, 596, 709
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 5 cut(s) 153, 274, 298, 615, 676
BshNI GGYRCC 1 cut(s) 923
BsiEI CGRYCG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 142, 275, 295, 322
BslFI GGGAC 4 cut(s) 143, 356, 646, 756
BslI CCNNNNNNNGG 1 cut(s) 623
BsmFI GGGAC 4 cut(s) 143, 356, 646, 756
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 5 cut(s) 153, 274, 298, 615, 676
Bsp143I GATC 5 cut(s) 78, 318, 409, 571, 654
Bsp19I CCATGG 2 cut(s) 123, 519
BspACI CCGC 4 cut(s) 299, 402, 664, 764
BspANI GGCC 5 cut(s) 153, 274, 298, 615, 676
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 3 cut(s) 146, 385, 925
BspPI GGATC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 923
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 3 cut(s) 379, 509, 616
BssECI CCNNGG 7 cut(s) 123, 148, 339, 387, 519, 927, 992
BssMI GATC 5 cut(s) 78, 318, 409, 571, 654
BssNI GRCGYC 1 cut(s) 970
BssT1I CCWWGG 3 cut(s) 123, 387, 519
Bst2UI CCWGG 3 cut(s) 340, 887, 994
Bst4CI ACNGT 2 cut(s) 757, 790
BstACI GRCGYC 1 cut(s) 970
BstAPI GCANNNNNTGC 2 cut(s) 556, 593
BstBAI YACGTR 1 cut(s) 689
BstDEI CTNAG 2 cut(s) 618, 812
BstDSI CCRYGG 3 cut(s) 123, 148, 519
BstF5I GGATG 2 cut(s) 133, 436
BstKTI GATC 5 cut(s) 81, 321, 412, 574, 657
BstMBI GATC 5 cut(s) 78, 318, 409, 571, 654
BstMCI CGRYCG 1 cut(s) 299
BstMWI GCNNNNNNNGC 5 cut(s) 524, 533, 556, 593, 728
BstNI CCWGG 3 cut(s) 340, 887, 994
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 4 cut(s) 321, 338, 885, 992
BstV1I GCAGC 3 cut(s) 172, 596, 709
BstV2I GAAGAC 2 cut(s) 491, 953
BstXI CCANNNNNNTGG 1 cut(s) 893
BstZI CGGCCG 1 cut(s) 296
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 5 cut(s) 153, 274, 298, 615, 676
BtgI CCRYGG 3 cut(s) 123, 148, 519
BtsCI GGATG 2 cut(s) 133, 436
BtsIMutI CAGTG 2 cut(s) 213, 753
CaiI CAGNNNCTG 1 cut(s) 725
CciI TCATGA 1 cut(s) 27
Cfr13I GGNCC 4 cut(s) 22, 144, 614, 674
Csp6I GTAC 1 cut(s) 12
CviAII CATG 7 cut(s) 28, 124, 520, 551, 562, 658, 875
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 2 cut(s) 618, 812
DpnI GATC 5 cut(s) 80, 320, 411, 573, 656
DpnII GATC 5 cut(s) 78, 318, 409, 571, 654
EaeI YGGCCR 2 cut(s) 151, 296
EagI CGGCCG 1 cut(s) 296
EclXI CGGCCG 1 cut(s) 296
Eco130I CCWWGG 3 cut(s) 123, 387, 519
Eco47I GGWCC 2 cut(s) 22, 144
Eco52I CGGCCG 1 cut(s) 296
Eco57I CTGAAG 2 cut(s) 464, 747
Eco72I CACGTG 1 cut(s) 689
Eco81I CCTNAGG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 22
EcoRII CCWGG 3 cut(s) 338, 885, 992
EcoT14I CCWWGG 3 cut(s) 123, 387, 519
ErhI CCWWGG 3 cut(s) 123, 387, 519
FaeI CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
FaqI GGGAC 4 cut(s) 143, 356, 646, 756
FatI CATG 7 cut(s) 27, 123, 519, 550, 561, 657, 874
FauI CCCGC 1 cut(s) 671
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 5 cut(s) 186, 299, 585, 723, 764
FokI GGATG 2 cut(s) 140, 443
Fsp4HI GCNGC 5 cut(s) 186, 299, 585, 723, 764
FspBI CTAG 1 cut(s) 704
GluI GCNGC 5 cut(s) 186, 299, 585, 723, 764
GsuI CTGGAG 1 cut(s) 869
HaeIII GGCC 5 cut(s) 153, 274, 298, 615, 676
HapII CCGG 4 cut(s) 142, 275, 295, 322
Hin1I GRCGYC 1 cut(s) 970
Hin1II CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
HincII GTYRAC 3 cut(s) 193, 751, 968
HindII GTYRAC 3 cut(s) 193, 751, 968
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 5 cut(s) 48, 163, 197, 490, 882
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 4 cut(s) 142, 275, 295, 322
HphI GGTGA 2 cut(s) 158, 419
Hpy166II GTNNAC 5 cut(s) 40, 193, 213, 751, 968
Hpy188I TCNGA 3 cut(s) 78, 442, 489
Hpy188III TCNNGA 4 cut(s) 28, 160, 803, 974
Hpy8I GTNNAC 5 cut(s) 40, 193, 213, 751, 968
Hpy99I CGWCG 4 cut(s) 242, 498, 969, 972
HpyAV CCTTC 7 cut(s) 13, 239, 276, 373, 488, 926, 935
HpyCH4III ACNGT 2 cut(s) 757, 790
HpyCH4IV ACGT 3 cut(s) 688, 964, 970
HpyCH4V TGCA 6 cut(s) 559, 584, 830, 860, 905, 961
HpyF10VI GCNNNNNNNGC 5 cut(s) 524, 533, 556, 593, 728
HpyF3I CTNAG 2 cut(s) 618, 812
HpySE526I ACGT 3 cut(s) 688, 964, 970
Hsp92I GRCGYC 1 cut(s) 970
Hsp92II CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
Ksp22I TGATCA 1 cut(s) 409
KspAI GTTAAC 1 cut(s) 751
Kzo9I GATC 5 cut(s) 78, 318, 409, 571, 654
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 3 cut(s) 172, 596, 709
LweI GCATC 2 cut(s) 325, 625
MaeI CTAG 1 cut(s) 704
MaeII ACGT 3 cut(s) 688, 964, 970
MalI GATC 5 cut(s) 80, 320, 411, 573, 656
MboI GATC 5 cut(s) 78, 318, 409, 571, 654
MboII GAAGA 4 cut(s) 57, 111, 491, 958
MluCI AATT 6 cut(s) 82, 87, 451, 513, 772, 831
MlyI GAGTC 3 cut(s) 157, 206, 876
MmeI TCCRAC 2 cut(s) 87, 604
MnlI CCTC 4 cut(s) 35, 64, 539, 922
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
MslI CAYNNNNRTG 1 cut(s) 758
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 4 cut(s) 142, 275, 295, 322
MspR9I CCNGG 4 cut(s) 323, 340, 887, 994
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 3 cut(s) 340, 887, 994
MwoI GCNNNNNNNGC 5 cut(s) 524, 533, 556, 593, 728
NciI CCSGG 1 cut(s) 323
NcoI CCATGG 2 cut(s) 123, 519
NdeII GATC 5 cut(s) 78, 318, 409, 571, 654
NlaIII CATG 7 cut(s) 31, 127, 523, 554, 565, 661, 878
NlaIV GGNNCC 3 cut(s) 146, 385, 925
NspI RCATGY 1 cut(s) 554
OliI CACNNNNGTG 1 cut(s) 758
PagI TCATGA 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 2 cut(s) 48, 490
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 5 cut(s) 187, 300, 586, 724, 765
PleI GAGTC 3 cut(s) 157, 205, 876
PmaCI CACGTG 1 cut(s) 689
PmlI CACGTG 1 cut(s) 689
PpsI GAGTC 3 cut(s) 157, 205, 876
Ppu21I YACGTR 1 cut(s) 689
PpuMI RGGWCCY 1 cut(s) 22
PshBI ATTAAT 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 3 cut(s) 338, 885, 992
PspCI CACGTG 1 cut(s) 689
PspGI CCWGG 3 cut(s) 338, 885, 992
PspN4I GGNNCC 3 cut(s) 146, 385, 925
PspPI GGNCC 4 cut(s) 22, 144, 614, 674
PspPPI RGGWCCY 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 725
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
RseI CAYNNNNRTG 1 cut(s) 758
SalI GTCGAC 1 cut(s) 966
SaqAI TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
SatI GCNGC 5 cut(s) 186, 299, 585, 723, 764
Sau3AI GATC 5 cut(s) 78, 318, 409, 571, 654
Sau96I GGNCC 4 cut(s) 22, 144, 614, 674
SchI GAGTC 3 cut(s) 157, 206, 876
ScrFI CCNGG 4 cut(s) 323, 340, 887, 994
SfaNI GCATC 2 cut(s) 325, 625
SgrDI CGTCGACG 1 cut(s) 966
SinI GGWCC 2 cut(s) 22, 144
SmiMI CAYNNNNRTG 1 cut(s) 758
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 6 cut(s) 82, 87, 451, 513, 772, 831
SsiI CCGC 4 cut(s) 299, 402, 664, 764
SspMI CTAG 1 cut(s) 704
StyD4I CCNGG 4 cut(s) 321, 338, 885, 992
StyI CCWWGG 3 cut(s) 123, 387, 519
TaaI ACNGT 2 cut(s) 757, 790
TaiI ACGT 3 cut(s) 691, 967, 973
TaqI TCGA 3 cut(s) 653, 802, 967
TasI AATT 6 cut(s) 82, 87, 451, 513, 772, 831
TatI WGTACW 1 cut(s) 11
TauI GCSGC 2 cut(s) 301, 766
TfiI GAWTC 2 cut(s) 48, 490
Tru1I TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
Tru9I TTAA 8 cut(s) 267, 309, 330, 465, 750, 792, 855, 1003
TscAI CASTG 2 cut(s) 220, 760
TseI GCWGC 3 cut(s) 185, 584, 722
TspDTI ATGAA 2 cut(s) 44, 954
TspGWI ACGGA 2 cut(s) 226, 443
TspRI CASTG 2 cut(s) 220, 760
VpaK11BI GGWCC 2 cut(s) 22, 144
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 2 cut(s) 451, 772
XceI RCATGY 1 cut(s) 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 967
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 1 cut(s) 704
ZraI GACGTC 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.