pycom13g08190

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013538
Physical Location & Seq
Reverse (-)
5440468 .. 5442626
2159 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g08190.1

Sequence Viewer

Length: 1008 bp
ATGGCAGAGGGCTTAAAGAAGCACGTCATGACCACCGCGACTAGGAGGGTGGCCTCCATCGCCAACCACCTCATTCCGGCCCATTTATCTCCCGGCTCCGCCGCAATTTCTCTCTCCAACGCCTCCATGAACGACAGCTACCACCGCACCCACGGCGAGGTCTCGACTCACCGAGTTGTTTGGGAAAATGCTCGCGACGTTTCCGGCGACGAATTCATTGATATTATATACGAGAAAGCCATCGGCGAAGCCATTGCAAAGGTTACGATTAATAGGCCGGAGAGAAGAAATGCGTTCCGGCCGCAGACTGTGAAGGAGCTGATTCGCGCGTTTAATGATGCCAGGGACGACGGCTCCATAGGCGTCGTCATTCTTACCGGGAAGGGAACCAAAGCATTCTGCAGTGGCGGTGATCAGGCTTTGAGAGGAAAATATGGTTATTCTGATTACGATGATTTTGGCCGTCTTAATGTGTTAGATTTGCAGGTCCAGATTCGACGTCTTCCAAAGCCAGTAATAGCCATGGTGGCTGGTTATGCTGTTGGGGGAGGACATGTGTTACACTTGGTCTGTGATCTAACCATTGCGGCAGACAATGCTATTTTTGGTCAGACTGGTCCTAAGGTTGGAAGCTTTGACGCTGGTTATGGGAGTTCCATCATGTCCCGTTTGGTCGGGCCTAAAAAAGCACGAGAAATGTGGTTTATGGCAAGGTTTTACACAGCTGCTGAAGCAGAGAAAATGGGACTTGTTAACACTGTTGTACCACTGGAGACTTTAGAGCAAGAAACGGTTAAATGGTGTCGAGAAATGCTAAGAAACAGCCCAACCGCGCTTCGGGTGCTGAAGTCTGCTCTTAATGCAGTTGACGACGGTCATGCTGGACTGCAGCAACTTGCTGGAGATGCCACGCTCTTATTCTATGGCACTGAGGAAGGTAACGAGGGGAAGACGGCTTATATGCAGCGCAGACGTCCAGATTTCTCAAAGTTTCCCAGGAGACCTTAA

Protein Analysis

336

Amino Acids

36.73

Weight (kDa)

9.14

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 82 - 329 1.2e-74 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 86 - 262 1.5e-20 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60550
fragaria_vesca FvH4_4g34130 FvH4_4g34130 FvH4_4g34130
malus_domestica MD13G1094000.v1.1 MD16G1095300.v1.1
prunus_persica Prupe.1G251300_v2.0.a1
pyrus_communis pycom13g08190
rosa_chinensis RchiOBHm_Chr4g0443641
rosa_laevigata RLG00000005906
rosa_multiflora Rmu_sc0001118.1_g000011 Rmu_sc0033917.1_g000002
rosa_roxburghii Rroxscaffold_5G00384190
rosa_samantha Rh4AG405800 Rh4BG416800 Rh4CG431300 Rh4DG412500
rosa_wichuraiana Rw4G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 502, 976
Acc36I ACCTGC 1 cut(s) 475
AccII CGCG 5 cut(s) 38, 195, 327, 329, 833
AciI CCGC 8 cut(s) 36, 99, 102, 145, 302, 408, 587, 831
AcoI YGGCCR 2 cut(s) 299, 460
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 2 cut(s) 750, 866
AcyI GRCGYC 3 cut(s) 363, 499, 973
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 5 cut(s) 42, 76, 157, 626, 837
AflIII ACRYGT 1 cut(s) 553
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 2 cut(s) 341, 995
AluBI AGCT 4 cut(s) 138, 319, 633, 725
AluI AGCT 4 cut(s) 138, 319, 633, 725
Alw26I GTCTC 3 cut(s) 166, 767, 994
AlwNI CAGNNNCTG 1 cut(s) 728
AoxI GGCC 6 cut(s) 51, 78, 275, 299, 460, 677
ApeKI GCWGC 3 cut(s) 725, 889, 964
ApoI RAATTY 1 cut(s) 212
AseI ATTAAT 1 cut(s) 270
AspLEI GCGC 3 cut(s) 329, 835, 969
AspS9I GGNCC 4 cut(s) 79, 487, 617, 677
AsuC2I CCSGG 2 cut(s) 93, 379
AsuHPI GGTGA 2 cut(s) 161, 422
AvaII GGWCC 2 cut(s) 487, 617
AxyI CCTNAGG 1 cut(s) 621
BaeI ACNNNNGTAYC 2 cut(s) 747, 780
BauI CACGAG 1 cut(s) 690
BbsI GAAGAC 2 cut(s) 494, 956
BbvI GCAGC 3 cut(s) 712, 901, 976
BccI CCATC 3 cut(s) 65, 248, 665
BceAI ACGGC 4 cut(s) 169, 367, 447, 969
BcgI CGANNNNNNTGC 4 cut(s) 236, 270, 860, 894
BciT130I CCWGG 2 cut(s) 343, 997
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 2 cut(s) 93, 379
BcoDI GTCTC 3 cut(s) 166, 767, 994
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 2 cut(s) 400, 887
BfuAI ACCTGC 1 cut(s) 475
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 6 cut(s) 102, 302, 588, 726, 890, 965
BlsI GCNGC 6 cut(s) 103, 303, 589, 727, 891, 966
Bme1390I CCNGG 4 cut(s) 93, 343, 379, 997
Bme18I GGWCC 2 cut(s) 487, 617
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 79, 487, 617, 677
BmiI GGNNCC 3 cut(s) 97, 355, 388
BmrFI CCNGG 4 cut(s) 93, 343, 379, 997
BmsI GCATC 2 cut(s) 328, 895
BoxI GACNNNNGTC 1 cut(s) 873
BpiI GAAGAC 2 cut(s) 494, 956
BpmI CTGGAG 2 cut(s) 791, 921
BpuMI CCSGG 2 cut(s) 93, 379
BsaHI GRCGYC 3 cut(s) 363, 499, 973
BsaI GGTCTC 2 cut(s) 166, 994
BsaJI CCNNGG 4 cut(s) 151, 342, 522, 995
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 540, 570
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 76, 157, 626, 837
Bse1I ACTGG 3 cut(s) 512, 619, 774
Bse21I CCTNAGG 1 cut(s) 621
Bse3DI GCAATG 2 cut(s) 252, 582
BseBI CCWGG 2 cut(s) 343, 997
BseDI CCNNGG 4 cut(s) 151, 342, 522, 995
BseLI CCNNNNNNNGG 5 cut(s) 42, 76, 157, 626, 837
BseMI GCAATG 2 cut(s) 252, 582
BseMII CTCAG 1 cut(s) 921
BseNI ACTGG 3 cut(s) 512, 619, 774
BseX3I CGGCCG 1 cut(s) 299
BseXI GCAGC 3 cut(s) 712, 901, 976
Bsh1236I CGCG 5 cut(s) 38, 195, 327, 329, 833
Bsh1285I CGRYCG 1 cut(s) 302
BshFI GGCC 6 cut(s) 53, 80, 277, 301, 462, 679
BsiEI CGRYCG 1 cut(s) 302
BsiSI CCGG 6 cut(s) 77, 93, 204, 278, 298, 378
BslFI GGGAC 3 cut(s) 359, 649, 759
BslI CCNNNNNNNGG 5 cut(s) 42, 76, 157, 626, 837
BsmAI GTCTC 3 cut(s) 166, 767, 994
BsmFI GGGAC 3 cut(s) 359, 649, 759
BsmI GAATGC 1 cut(s) 395
BsnI GGCC 6 cut(s) 53, 80, 277, 301, 462, 679
Bso31I GGTCTC 2 cut(s) 166, 994
Bsp143I GATC 2 cut(s) 412, 574
Bsp19I CCATGG 1 cut(s) 522
Bsp68I TCGCGA 1 cut(s) 195
BspACI CCGC 8 cut(s) 36, 99, 102, 145, 302, 408, 587, 831
BspANI GGCC 6 cut(s) 53, 80, 277, 301, 462, 679
BspCNI CTCAG 1 cut(s) 922
BspFNI CGCG 5 cut(s) 38, 195, 327, 329, 833
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 3 cut(s) 97, 355, 388
BspMAI CTGCAG 2 cut(s) 404, 891
BspMI ACCTGC 1 cut(s) 475
BspTNI GGTCTC 2 cut(s) 166, 994
BsrDI GCAATG 2 cut(s) 252, 582
BsrI ACTGG 3 cut(s) 512, 619, 774
BssECI CCNNGG 4 cut(s) 151, 342, 522, 995
BssMI GATC 2 cut(s) 412, 574
BssNI GRCGYC 3 cut(s) 363, 499, 973
BssSI CACGAG 1 cut(s) 690
BssT1I CCWWGG 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 690
Bst2UI CCWGG 2 cut(s) 343, 997
Bst4CI ACNGT 4 cut(s) 310, 760, 793, 875
BstACI GRCGYC 3 cut(s) 363, 499, 973
BstAPI GCANNNNNTGC 1 cut(s) 596
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 621, 815, 930
BstDSI CCRYGG 2 cut(s) 151, 522
BstFNI CGCG 5 cut(s) 38, 195, 327, 329, 833
BstHHI GCGC 3 cut(s) 329, 835, 969
BstKTI GATC 2 cut(s) 415, 577
BstMAI GTCTC 3 cut(s) 166, 767, 994
BstMBI GATC 2 cut(s) 412, 574
BstMCI CGRYCG 1 cut(s) 302
BstNI CCWGG 2 cut(s) 343, 997
BstNSI RCATGY 1 cut(s) 557
BstPAI GACNNNNGTC 1 cut(s) 873
BstSCI CCNGG 4 cut(s) 91, 341, 377, 995
BstSFI CTRYAG 2 cut(s) 400, 887
BstUI CGCG 5 cut(s) 38, 195, 327, 329, 833
BstV1I GCAGC 3 cut(s) 712, 901, 976
BstV2I GAAGAC 2 cut(s) 494, 956
BstZI CGGCCG 1 cut(s) 299
Bsu36I CCTNAGG 1 cut(s) 621
BsuRI GGCC 6 cut(s) 53, 80, 277, 301, 462, 679
BtgI CCRYGG 2 cut(s) 151, 522
BtgZI GCGATG 1 cut(s) 43
BtrI CACGTC 1 cut(s) 25
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 4 cut(s) 409, 756, 767, 927
BtuMI TCGCGA 1 cut(s) 195
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 193
CaiI CAGNNNCTG 1 cut(s) 728
CciI TCATGA 1 cut(s) 27
CfoI GCGC 3 cut(s) 329, 835, 969
Cfr13I GGNCC 4 cut(s) 79, 487, 617, 677
CseI GACGC 2 cut(s) 352, 647
Csp6I GTAC 1 cut(s) 764
CviAII CATG 6 cut(s) 28, 127, 523, 554, 661, 878
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 3 cut(s) 621, 815, 930
DpnI GATC 2 cut(s) 414, 576
DpnII GATC 2 cut(s) 412, 574
EaeI YGGCCR 2 cut(s) 299, 460
EagI CGGCCG 1 cut(s) 299
EciI GGCGGA 1 cut(s) 88
EclXI CGGCCG 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 522
Eco31I GGTCTC 2 cut(s) 166, 994
Eco47I GGWCC 2 cut(s) 487, 617
Eco52I CGGCCG 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 750, 866
Eco81I CCTNAGG 1 cut(s) 621
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 341, 995
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 6 cut(s) 31, 130, 526, 557, 664, 881
FaqI GGGAC 3 cut(s) 359, 649, 759
FatI CATG 6 cut(s) 27, 126, 522, 553, 660, 877
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 6 cut(s) 102, 302, 588, 726, 890, 965
Fsp4HI GCNGC 6 cut(s) 102, 302, 588, 726, 890, 965
FspBI CTAG 1 cut(s) 42
GlaI GCGC 3 cut(s) 328, 834, 968
GluI GCNGC 6 cut(s) 102, 302, 588, 726, 890, 965
GsuI CTGGAG 2 cut(s) 791, 921
HaeIII GGCC 6 cut(s) 53, 80, 277, 301, 462, 679
HapII CCGG 6 cut(s) 77, 93, 204, 278, 298, 378
HgaI GACGC 2 cut(s) 352, 647
HhaI GCGC 3 cut(s) 329, 835, 969
Hin1I GRCGYC 3 cut(s) 363, 499, 973
Hin1II CATG 6 cut(s) 31, 130, 526, 557, 664, 881
Hin6I GCGC 3 cut(s) 327, 833, 967
HinP1I GCGC 3 cut(s) 327, 833, 967
HincII GTYRAC 2 cut(s) 754, 868
HindII GTYRAC 2 cut(s) 754, 868
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 3 cut(s) 166, 322, 493
HpaI GTTAAC 1 cut(s) 754
HpaII CCGG 6 cut(s) 77, 93, 204, 278, 298, 378
HphI GGTGA 2 cut(s) 161, 422
Hpy166II GTNNAC 2 cut(s) 754, 868
Hpy188I TCNGA 2 cut(s) 445, 612
Hpy188III TCNNGA 6 cut(s) 28, 163, 194, 490, 806, 977
Hpy8I GTNNAC 2 cut(s) 754, 868
Hpy99I CGWCG 6 cut(s) 200, 212, 353, 368, 501, 875
HpyAV CCTTC 3 cut(s) 307, 376, 929
HpyCH4III ACNGT 4 cut(s) 310, 760, 793, 875
HpyCH4IV ACGT 4 cut(s) 24, 198, 499, 973
HpyCH4V TGCA 6 cut(s) 257, 402, 484, 863, 889, 964
HpyF3I CTNAG 3 cut(s) 621, 815, 930
HpySE526I ACGT 4 cut(s) 24, 198, 499, 973
Hsp92I GRCGYC 3 cut(s) 363, 499, 973
Hsp92II CATG 6 cut(s) 31, 130, 526, 557, 664, 881
HspAI GCGC 3 cut(s) 327, 833, 967
Ksp22I TGATCA 1 cut(s) 412
KspAI GTTAAC 1 cut(s) 754
Kzo9I GATC 2 cut(s) 412, 574
LmnI GCTCC 3 cut(s) 101, 316, 359
Lsp1109I GCAGC 3 cut(s) 712, 901, 976
LweI GCATC 2 cut(s) 328, 895
MaeI CTAG 1 cut(s) 42
MaeII ACGT 4 cut(s) 24, 198, 499, 973
MaeIII GTNAC 3 cut(s) 262, 558, 938
MalI GATC 2 cut(s) 414, 576
MboI GATC 2 cut(s) 412, 574
MboII GAAGA 3 cut(s) 297, 494, 961
MluCI AATT 2 cut(s) 105, 212
MlyI GAGTC 1 cut(s) 160
MmeI TCCRAC 2 cut(s) 141, 607
MnlI CCTC 9 cut(s) 39, 64, 80, 133, 151, 419, 542, 925, 937
MseI TTAA 8 cut(s) 14, 270, 333, 468, 753, 795, 858, 1006
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 6 cut(s) 77, 93, 204, 278, 298, 378
MspR9I CCNGG 4 cut(s) 93, 343, 379, 997
Mva1269I GAATGC 1 cut(s) 395
MvaI CCWGG 2 cut(s) 343, 997
MvnI CGCG 5 cut(s) 38, 195, 327, 329, 833
NciI CCSGG 2 cut(s) 93, 379
NcoI CCATGG 1 cut(s) 522
NdeII GATC 2 cut(s) 412, 574
NlaIII CATG 6 cut(s) 31, 130, 526, 557, 664, 881
NlaIV GGNNCC 3 cut(s) 97, 355, 388
NruI TCGCGA 1 cut(s) 195
NspI RCATGY 1 cut(s) 557
PagI TCATGA 1 cut(s) 27
PciI ACATGT 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 2 cut(s) 322, 493
PkrI GCNGC 6 cut(s) 103, 303, 589, 727, 891, 966
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
PscI ACATGT 1 cut(s) 553
PshAI GACNNNNGTC 1 cut(s) 873
PshBI ATTAAT 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 341, 995
PspGI CCWGG 2 cut(s) 341, 995
PspN4I GGNNCC 3 cut(s) 97, 355, 388
PspPI GGNCC 4 cut(s) 79, 487, 617, 677
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
PstI CTGCAG 2 cut(s) 404, 891
PstNI CAGNNNCTG 1 cut(s) 728
PvuII CAGCTG 1 cut(s) 725
RruI TCGCGA 1 cut(s) 195
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
SaqAI TTAA 8 cut(s) 14, 270, 333, 468, 753, 795, 858, 1006
SatI GCNGC 6 cut(s) 102, 302, 588, 726, 890, 965
Sau3AI GATC 2 cut(s) 412, 574
Sau96I GGNCC 4 cut(s) 79, 487, 617, 677
SchI GAGTC 1 cut(s) 160
ScrFI CCNGG 4 cut(s) 93, 343, 379, 997
SfaNI GCATC 2 cut(s) 328, 895
SfcI CTRYAG 2 cut(s) 400, 887
SinI GGWCC 2 cut(s) 487, 617
Sse9I AATT 2 cut(s) 105, 212
SsiI CCGC 8 cut(s) 36, 99, 102, 145, 302, 408, 587, 831
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 4 cut(s) 91, 341, 377, 995
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 4 cut(s) 310, 760, 793, 875
TaiI ACGT 4 cut(s) 27, 201, 502, 976
TaqI TCGA 3 cut(s) 164, 496, 805
TasI AATT 2 cut(s) 105, 212
TauI GCSGC 3 cut(s) 104, 304, 590
TfiI GAWTC 2 cut(s) 322, 493
Tru1I TTAA 8 cut(s) 14, 270, 333, 468, 753, 795, 858, 1006
Tru9I TTAA 8 cut(s) 14, 270, 333, 468, 753, 795, 858, 1006
TscAI CASTG 4 cut(s) 409, 763, 774, 934
TseI GCWGC 3 cut(s) 725, 889, 964
TspDTI ATGAA 2 cut(s) 143, 205
TspRI CASTG 4 cut(s) 409, 763, 774, 934
VpaK11BI GGWCC 2 cut(s) 487, 617
VspI ATTAAT 1 cut(s) 270
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 557
XspI CTAG 1 cut(s) 42
ZraI GACGTC 2 cut(s) 500, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.