Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0013538

Physical Location & Seq

Genomic Coordinates Forward (+)

61795049 .. 61797607

Gene Structure 2559 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1014 bp

ATGGCTCAAAGTACAGAGTCAGAGAAGGACCTCATGATCAACAGAGTGAACAGAAGAATGGCCTCCATCTCCAACCATCTGACTCCGATCAGTTCAATTCCCAACTCCATTACTCTGTCGCACACATCTTCCATGGACGACAGCTACCACCGGACCCACGGCCAAGTCCCGACTCACCAAGTCGTCTGGGAAGCTGCTGTTGACGAGTCCGGCAAGGAGTTCACTGACATTATCTACGAGAAATCCGTCAGCGAAGGAATAGCAAAGATTACGATTAATAGGCCGGAGAGAAGGAATGCGTTCCGGCCGCAGACGATCAAGGAGCTGATCCGGGCTTTTAATGACGCCAGGGACGACAGTTCCATTGGCGTTATTGTTCTTACTGGGAAGGGAACCAAGGCATTTTGTAGCGGTGGTGATCAGGCTTTGAGAAGCACGGATGGTTATTCTGATTTTGAAAATTTTGGTCGTCTTAATGTTTTAGACCTTCAGGTTCAGATTCGTCGTCTTCCAAAGCCAGTAATTGCCATGGTGGCTGGTTATGCTGTTGGGGGAGGGCATGTGTTGCACATGGTATGTGATCTAACCATTGCAGCAGACAATGCTATTTTTGGTCAAACTGGCCCTAAGGTTGGAAGCTTTGACGCTGGTTATGGGAGTTCGATCATGTCCCGCTTGGTCGGGCCTAAAAAAGCACGTGAAATGTGGTTTCTAGCAAGGTTTTACACAGCTGCTGAAGCAGAGAAAATGGGACTTGTTAACACTGTTGTACCGCTCGAGAATTTAGAGCAAGAAACGGTTAAATGGTGTCGAGAAATCCTAAGAAACAGCCCAACTGCAATTCGGGTGTTGAAATCATCTCTTAATGCAGTTGATGATGGTCATGCTGGACTCCAGGAACTTGGTGGAAATGCAACACTCATATTCTATGGCACTCAGGAAGGAAATGAAGGGAAGACAGCATATATGCAACGTCGACGTCCAGATTTCACAAAGTTTCCCAGGCGACCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0008935 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016829 GO:0016830 GO:0016833 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0051186 GO:0051188 GO:0071704 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

ECH_1

Protein Analysis

337

Amino Acids

37.22

Weight (kDa)

8.67

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	84 - 331	6.3e-74	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	88 - 264	1.5e-21	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013538)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60550
fragaria_vesca	FvH4_4g34130 FvH4_4g34130 FvH4_4g34130
malus_domestica	MD13G1094000.v1.1 MD16G1095300.v1.1
prunus_persica	Prupe.1G251300_v2.0.a1
pyrus_communis	pycom13g08190
rosa_chinensis	RchiOBHm_Chr4g0443641
rosa_laevigata	RLG00000005906
rosa_multiflora	Rmu_sc0001118.1_g000011 Rmu_sc0033917.1_g000002
rosa_roxburghii	Rroxscaffold_5G00384190
rosa_samantha	Rh4AG405800 Rh4BG416800 Rh4CG431300 Rh4DG412500
rosa_wichuraiana	Rw4G034840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	982
AccBSI	CCGCTC	1 cut(s)	775
AccI	GTMKAC	1 cut(s)	976
AciI	CCGC	4 cut(s)	308, 411, 673, 773
AclWI	GGATC	1 cut(s)	322
AcoI	YGGCCR	2 cut(s)	160, 305
AcsI	RAATTY	2 cut(s)	460, 781
AcuI	CTGAAG	2 cut(s)	473, 756
AcvI	CACGTG	1 cut(s)	698
AcyI	GRCGYC	2 cut(s)	345, 979
AfaI	GTAC	2 cut(s)	13, 771
AfiI	CCNNNNNNNGG	1 cut(s)	632
AgsI	TTSAA	3 cut(s)	96, 458, 853
AjnI	CCWGG	3 cut(s)	347, 894, 1001
AluBI	AGCT	5 cut(s)	144, 194, 325, 639, 731
AluI	AGCT	5 cut(s)	144, 194, 325, 639, 731
AlwI	GGATC	1 cut(s)	322
AlwNI	CAGNNNCTG	1 cut(s)	734
Ama87I	CYCGRG	1 cut(s)	776
AoxI	GGCC	6 cut(s)	60, 160, 281, 305, 622, 683
ApeKI	GCWGC	3 cut(s)	194, 593, 731
ApoI	RAATTY	2 cut(s)	460, 781
AseI	ATTAAT	1 cut(s)	276
Asp700I	GAANNNNTTC	1 cut(s)	299
AspS9I	GGNCC	4 cut(s)	28, 153, 623, 683
AsuC2I	CCSGG	1 cut(s)	332
AsuHPI	GGTGA	2 cut(s)	167, 428
AvaI	CYCGRG	1 cut(s)	776
AvaII	GGWCC	2 cut(s)	28, 153
AxyI	CCTNAGG	1 cut(s)	627
BaeI	ACNNNNGTAYC	2 cut(s)	753, 786
BbrPI	CACGTG	1 cut(s)	698
BbsI	GAAGAC	2 cut(s)	500, 962
BbvI	GCAGC	3 cut(s)	181, 605, 718
BccI	CCATC	4 cut(s)	74, 84, 434, 872
BceAI	ACGGC	1 cut(s)	175
BciT130I	CCWGG	3 cut(s)	349, 896, 1003
BclI	TGATCA	2 cut(s)	36, 418
BcnI	CCSGG	1 cut(s)	332
BfaI	CTAG	1 cut(s)	713
BglI	GCCNNNNNGGC	1 cut(s)	533
BisI	GCNGC	4 cut(s)	195, 308, 594, 732
BlsI	GCNGC	4 cut(s)	196, 309, 595, 733
Bme1390I	CCNGG	4 cut(s)	332, 349, 896, 1003
Bme18I	GGWCC	2 cut(s)	28, 153
BmeT110I	CYCGRG	1 cut(s)	776
BmgT120I	GGNCC	4 cut(s)	28, 153, 623, 683
BmiI	GGNNCC	2 cut(s)	155, 394
BmrFI	CCNGG	4 cut(s)	332, 349, 896, 1003
BmrI	ACTGGG	1 cut(s)	393
BmuI	ACTGGG	1 cut(s)	393
BpiI	GAAGAC	2 cut(s)	500, 962
BpmI	CTGGAG	1 cut(s)	878
BpuMI	CCSGG	1 cut(s)	332
BsaAI	YACGTR	1 cut(s)	698
BsaHI	GRCGYC	2 cut(s)	345, 979
BsaJI	CCNNGG	6 cut(s)	132, 157, 348, 396, 528, 1001
BsaWI	WCCGGW	1 cut(s)	150
BsaXI	ACNNNNNCTCC	2 cut(s)	546, 576
Bsc4I	CCNNNNNNNGG	1 cut(s)	632
Bse1I	ACTGG	3 cut(s)	388, 518, 625
Bse21I	CCTNAGG	1 cut(s)	627
Bse3DI	GCAATG	1 cut(s)	588
BseBI	CCWGG	3 cut(s)	349, 896, 1003
BseDI	CCNNGG	6 cut(s)	132, 157, 348, 396, 528, 1001
BseGI	GGATG	1 cut(s)	445
BseLI	CCNNNNNNNGG	1 cut(s)	632
BseMI	GCAATG	1 cut(s)	588
BseMII	CTCAG	1 cut(s)	950
BseNI	ACTGG	3 cut(s)	388, 518, 625
BseX3I	CGGCCG	1 cut(s)	305
BseXI	GCAGC	3 cut(s)	181, 605, 718
Bsh1285I	CGRYCG	1 cut(s)	308
BshFI	GGCC	6 cut(s)	62, 162, 283, 307, 624, 685
BsiEI	CGRYCG	1 cut(s)	308
BsiHKCI	CYCGRG	1 cut(s)	776
BsiSI	CCGG	5 cut(s)	151, 210, 284, 304, 331
BslFI	GGGAC	4 cut(s)	152, 365, 655, 765
BslI	CCNNNNNNNGG	1 cut(s)	632
BsmFI	GGGAC	4 cut(s)	152, 365, 655, 765
BsmI	GAATGC	1 cut(s)	301
BsnI	GGCC	6 cut(s)	62, 162, 283, 307, 624, 685
BsoBI	CYCGRG	1 cut(s)	776
Bsp143I	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
Bsp19I	CCATGG	2 cut(s)	132, 528
BspACI	CCGC	4 cut(s)	308, 411, 673, 773
BspANI	GGCC	6 cut(s)	62, 162, 283, 307, 624, 685
BspCNI	CTCAG	1 cut(s)	949
BspHI	TCATGA	1 cut(s)	33
BspLI	GGNNCC	2 cut(s)	155, 394
BspPI	GGATC	1 cut(s)	322
BsrBI	CCGCTC	1 cut(s)	775
BsrDI	GCAATG	1 cut(s)	588
BsrI	ACTGG	3 cut(s)	388, 518, 625
BssECI	CCNNGG	6 cut(s)	132, 157, 348, 396, 528, 1001
BssMI	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
BssNI	GRCGYC	2 cut(s)	345, 979
BssT1I	CCWWGG	3 cut(s)	132, 396, 528
Bst2UI	CCWGG	3 cut(s)	349, 896, 1003
Bst4CI	ACNGT	3 cut(s)	359, 766, 799
BstACI	GRCGYC	2 cut(s)	345, 979
BstAPI	GCANNNNNTGC	2 cut(s)	565, 602
BstBAI	YACGTR	1 cut(s)	698
BstDEI	CTNAG	3 cut(s)	627, 821, 936
BstDSI	CCRYGG	3 cut(s)	132, 157, 528
BstF5I	GGATG	1 cut(s)	445
BstKTI	GATC	7 cut(s)	39, 90, 318, 330, 421, 583, 666
BstMBI	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
BstMCI	CGRYCG	1 cut(s)	308
BstMWI	GCNNNNNNNGC	5 cut(s)	533, 542, 565, 602, 737
BstNI	CCWGG	3 cut(s)	349, 896, 1003
BstNSI	RCATGY	1 cut(s)	563
BstSCI	CCNGG	4 cut(s)	330, 347, 894, 1001
BstV1I	GCAGC	3 cut(s)	181, 605, 718
BstV2I	GAAGAC	2 cut(s)	500, 962
BstXI	CCANNNNNNTGG	1 cut(s)	902
BstZI	CGGCCG	1 cut(s)	305
Bsu36I	CCTNAGG	1 cut(s)	627
BsuRI	GGCC	6 cut(s)	62, 162, 283, 307, 624, 685
BtgI	CCRYGG	3 cut(s)	132, 157, 528
BtsCI	GGATG	1 cut(s)	445
BtsIMutI	CAGTG	2 cut(s)	222, 762
CaiI	CAGNNNCTG	1 cut(s)	734
CciI	TCATGA	1 cut(s)	33
Cfr13I	GGNCC	4 cut(s)	28, 153, 623, 683
CseI	GACGC	2 cut(s)	353, 653
Csp6I	GTAC	2 cut(s)	12, 770
CviAII	CATG	7 cut(s)	34, 133, 529, 560, 571, 667, 884
CviQI	GTAC	2 cut(s)	12, 770
DdeI	CTNAG	3 cut(s)	627, 821, 936
DpnI	GATC	7 cut(s)	38, 89, 317, 329, 420, 582, 665
DpnII	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
EaeI	YGGCCR	2 cut(s)	160, 305
EagI	CGGCCG	1 cut(s)	305
EclXI	CGGCCG	1 cut(s)	305
Eco130I	CCWWGG	3 cut(s)	132, 396, 528
Eco47I	GGWCC	2 cut(s)	28, 153
Eco52I	CGGCCG	1 cut(s)	305
Eco57I	CTGAAG	2 cut(s)	473, 756
Eco72I	CACGTG	1 cut(s)	698
Eco81I	CCTNAGG	1 cut(s)	627
Eco88I	CYCGRG	1 cut(s)	776
EcoO109I	RGGNCCY	1 cut(s)	28
EcoRII	CCWGG	3 cut(s)	347, 894, 1001
EcoT14I	CCWWGG	3 cut(s)	132, 396, 528
ErhI	CCWWGG	3 cut(s)	132, 396, 528
FaeI	CATG	7 cut(s)	37, 136, 532, 563, 574, 670, 887
FaqI	GGGAC	4 cut(s)	152, 365, 655, 765
FatI	CATG	7 cut(s)	33, 132, 528, 559, 570, 666, 883
FauI	CCCGC	1 cut(s)	680
FbaI	TGATCA	2 cut(s)	36, 418
FblI	GTMKAC	1 cut(s)	976
Fnu4HI	GCNGC	4 cut(s)	195, 308, 594, 732
FokI	GGATG	1 cut(s)	452
Fsp4HI	GCNGC	4 cut(s)	195, 308, 594, 732
FspBI	CTAG	1 cut(s)	713
GluI	GCNGC	4 cut(s)	195, 308, 594, 732
GsuI	CTGGAG	1 cut(s)	878
HaeIII	GGCC	6 cut(s)	62, 162, 283, 307, 624, 685
HapII	CCGG	5 cut(s)	151, 210, 284, 304, 331
HgaI	GACGC	2 cut(s)	353, 653
Hin1I	GRCGYC	2 cut(s)	345, 979
Hin1II	CATG	7 cut(s)	37, 136, 532, 563, 574, 670, 887
HincII	GTYRAC	3 cut(s)	202, 760, 977
HindII	GTYRAC	3 cut(s)	202, 760, 977
HindIII	AAGCTT	1 cut(s)	637
HinfI	GANTC	6 cut(s)	17, 82, 172, 206, 499, 891
HpaI	GTTAAC	1 cut(s)	760
HpaII	CCGG	5 cut(s)	151, 210, 284, 304, 331
HphI	GGTGA	2 cut(s)	167, 428
Hpy166II	GTNNAC	5 cut(s)	49, 202, 222, 760, 977
Hpy188I	TCNGA	5 cut(s)	22, 81, 87, 451, 498
Hpy188III	TCNNGA	6 cut(s)	34, 169, 778, 812, 938, 983
Hpy8I	GTNNAC	5 cut(s)	49, 202, 222, 760, 977
Hpy99I	CGWCG	3 cut(s)	507, 978, 981
HpyAV	CCTTC	7 cut(s)	19, 248, 285, 382, 497, 935, 944
HpyCH4III	ACNGT	3 cut(s)	359, 766, 799
HpyCH4IV	ACGT	3 cut(s)	697, 973, 979
HpyCH4V	TGCA	6 cut(s)	568, 593, 839, 869, 914, 970
HpyF10VI	GCNNNNNNNGC	5 cut(s)	533, 542, 565, 602, 737
HpyF3I	CTNAG	3 cut(s)	627, 821, 936
HpySE526I	ACGT	3 cut(s)	697, 973, 979
Hsp92I	GRCGYC	2 cut(s)	345, 979
Hsp92II	CATG	7 cut(s)	37, 136, 532, 563, 574, 670, 887
Ksp22I	TGATCA	2 cut(s)	36, 418
KspAI	GTTAAC	1 cut(s)	760
Kzo9I	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
LmnI	GCTCC	1 cut(s)	322
Lsp1109I	GCAGC	3 cut(s)	181, 605, 718
MaeI	CTAG	1 cut(s)	713
MaeII	ACGT	3 cut(s)	697, 973, 979
MalI	GATC	7 cut(s)	38, 89, 317, 329, 420, 582, 665
MbiI	CCGCTC	1 cut(s)	775
MboI	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
MboII	GAAGA	4 cut(s)	66, 120, 500, 967
MluCI	AATT	5 cut(s)	96, 460, 522, 781, 840
MlyI	GAGTC	5 cut(s)	26, 76, 166, 215, 885
MmeI	TCCRAC	2 cut(s)	96, 613
MnlI	CCTC	3 cut(s)	41, 73, 548
MroXI	GAANNNNTTC	1 cut(s)	299
MseI	TTAA	7 cut(s)	276, 339, 474, 759, 801, 864, 1012
MspA1I	CMGCKG	1 cut(s)	731
MspI	CCGG	5 cut(s)	151, 210, 284, 304, 331
MspR9I	CCNGG	4 cut(s)	332, 349, 896, 1003
Mva1269I	GAATGC	1 cut(s)	301
MvaI	CCWGG	3 cut(s)	349, 896, 1003
MwoI	GCNNNNNNNGC	5 cut(s)	533, 542, 565, 602, 737
NciI	CCSGG	1 cut(s)	332
NcoI	CCATGG	2 cut(s)	132, 528
NdeII	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
NlaIII	CATG	7 cut(s)	37, 136, 532, 563, 574, 670, 887
NlaIV	GGNNCC	2 cut(s)	155, 394
NspI	RCATGY	1 cut(s)	563
PaeR7I	CTCGAG	1 cut(s)	776
PagI	TCATGA	1 cut(s)	33
PcsI	WCGNNNNNNNCGW	2 cut(s)	243, 351
PctI	GAATGC	1 cut(s)	301
PdmI	GAANNNNTTC	1 cut(s)	299
PfeI	GAWTC	1 cut(s)	499
PfoI	TCCNGGA	1 cut(s)	894
PkrI	GCNGC	4 cut(s)	196, 309, 595, 733
PleI	GAGTC	5 cut(s)	25, 76, 166, 214, 885
PmaCI	CACGTG	1 cut(s)	698
PmlI	CACGTG	1 cut(s)	698
PpsI	GAGTC	5 cut(s)	25, 76, 166, 214, 885
Ppu21I	YACGTR	1 cut(s)	698
PpuMI	RGGWCCY	1 cut(s)	28
PshBI	ATTAAT	1 cut(s)	276
Psp5II	RGGWCCY	1 cut(s)	28
Psp6I	CCWGG	3 cut(s)	347, 894, 1001
PspCI	CACGTG	1 cut(s)	698
PspGI	CCWGG	3 cut(s)	347, 894, 1001
PspN4I	GGNNCC	2 cut(s)	155, 394
PspPI	GGNCC	4 cut(s)	28, 153, 623, 683
PspPPI	RGGWCCY	1 cut(s)	28
PstNI	CAGNNNCTG	1 cut(s)	734
PvuII	CAGCTG	1 cut(s)	731
RsaI	GTAC	2 cut(s)	13, 771
RsaNI	GTAC	2 cut(s)	12, 770
SalI	GTCGAC	1 cut(s)	975
SaqAI	TTAA	7 cut(s)	276, 339, 474, 759, 801, 864, 1012
SatI	GCNGC	4 cut(s)	195, 308, 594, 732
Sau3AI	GATC	7 cut(s)	36, 87, 315, 327, 418, 580, 663
Sau96I	GGNCC	4 cut(s)	28, 153, 623, 683
SchI	GAGTC	5 cut(s)	26, 76, 166, 215, 885
ScrFI	CCNGG	4 cut(s)	332, 349, 896, 1003
Sfr274I	CTCGAG	1 cut(s)	776
SgrDI	CGTCGACG	1 cut(s)	975
SinI	GGWCC	2 cut(s)	28, 153
SlaI	CTCGAG	1 cut(s)	776
SmlI	CTYRAG	1 cut(s)	776
SmoI	CTYRAG	1 cut(s)	776
Sse9I	AATT	5 cut(s)	96, 460, 522, 781, 840
SsiI	CCGC	4 cut(s)	308, 411, 673, 773
SspMI	CTAG	1 cut(s)	713
StyD4I	CCNGG	4 cut(s)	330, 347, 894, 1001
StyI	CCWWGG	3 cut(s)	132, 396, 528
TaaI	ACNGT	3 cut(s)	359, 766, 799
TaiI	ACGT	3 cut(s)	700, 976, 982
TaqI	TCGA	4 cut(s)	662, 777, 811, 976
TasI	AATT	5 cut(s)	96, 460, 522, 781, 840
TatI	WGTACW	1 cut(s)	11
TauI	GCSGC	1 cut(s)	310
TfiI	GAWTC	1 cut(s)	499
Tru1I	TTAA	7 cut(s)	276, 339, 474, 759, 801, 864, 1012
Tru9I	TTAA	7 cut(s)	276, 339, 474, 759, 801, 864, 1012
TscAI	CASTG	2 cut(s)	229, 769
TseI	GCWGC	3 cut(s)	194, 593, 731
TspDTI	ATGAA	1 cut(s)	963
TspGWI	ACGGA	2 cut(s)	235, 452
TspRI	CASTG	2 cut(s)	229, 769
VpaK11BI	GGWCC	2 cut(s)	28, 153
VspI	ATTAAT	1 cut(s)	276
XapI	RAATTY	2 cut(s)	460, 781
XceI	RCATGY	1 cut(s)	563
XcmI	CCANNNNNNNNNTGG	1 cut(s)	902
XhoI	CTCGAG	1 cut(s)	776
XmiI	GTMKAC	1 cut(s)	976
XmnI	GAANNNNTTC	1 cut(s)	299
XspI	CTAG	1 cut(s)	713
ZraI	GACGTC	1 cut(s)	980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.