MD16G1112000.v1.1

membrane protein YuiD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010674
Physical Location & Seq
Forward (+)
7861281 .. 7863451
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1112000.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGTTGAGGCGGCGGCATCATCATCGTCCTCAATCTTCACCAACTTCCCTCTCCTCTCTGCTCTCGTAGCTTTCTCCATTGCCCAATTCATCAAGGTCTTCACCGCCTGGTACAAGGAAAGAAGATGGGATTTCAAGCAACTTCTTGGATCCGGCGGAATGCCATCATCTCATTCTGCGACTGTTACTGCTATTGCTACAGCCGTTGGGTTCCAAGAAGGCATCGGTGGATCACTCTTTGCCATTGCATTGGTCTTAGCATGTGTGGTGATGTATGATGCAACCGGTGTAAGATTACACGCTGGACGCCAAGCTGAGGTTTTGAATCAAATTGTGTATGAACTCCCTGCTGAACATCCTCTGGCTGAAAGCAGACCACTTCGAGAACTTCTCGGCCACACCCCTCTTCAGGTGGTTGCTGGCGGTTTGCTTGGAATTGTCACAGCAAGCATGGGCTATGTGATTACTCTTACTATCAGTCATACAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

164

Amino Acids

17.29

Weight (kDa)

6.5

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 14 - 147 1.4e-52 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24350 AT1G24350 AT1G24350 AT1G24350 AT1G24350 AT1G67600
fragaria_vesca FvH4_4g24302
malus_domestica MD13G1113100.v1.1 MD16G1112000.v1.1
prunus_persica Prupe.1G233000_v2.0.a1
pyrus_communis pycom13g09850 pycom16g09500
rosa_chinensis RchiOBHm_Chr4g0431101
rosa_laevigata RLG00000006925
rosa_multiflora Rmu_co8474717.1_g000001 Rmu_sc0010203.1_g000012
rosa_roxburghii Rroxscaffold_5G00372690
rosa_rugosa Rorug04G0248000
rosa_samantha Rh4DG308400
rosa_wichuraiana Rw4G026470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 11, 14, 105, 156, 423
AclWI GGATC 3 cut(s) 144, 157, 238
AcoI YGGCCR 1 cut(s) 394
AcuI CTGAAG 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 307
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 316, 409
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 2 cut(s) 136, 325
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 2 cut(s) 71, 314
AluI AGCT 2 cut(s) 71, 314
AlwI GGATC 3 cut(s) 144, 157, 238
AoxI GGCC 1 cut(s) 394
AsiGI ACCGGT 1 cut(s) 284
AsuHPI GGTGA 3 cut(s) 31, 94, 280
BamHI GGATCC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 91
BbvCI CCTCAGC 1 cut(s) 315
BccI CCATC 2 cut(s) 120, 172
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 109
BfmI CTRYAG 1 cut(s) 198
BisI GCNGC 2 cut(s) 12, 15
BlsI GCNGC 2 cut(s) 13, 16
Bme1390I CCNGG 1 cut(s) 109
BmiI GGNNCC 2 cut(s) 151, 212
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 3 cut(s) 26, 231, 268
BpiI GAAGAC 1 cut(s) 91
BplI GAGNNNNNCTC 2 cut(s) 375, 407
Bpu10I CCTNAGC 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 307
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 409
Bse118I RCCGGY 1 cut(s) 284
Bse3DI GCAATG 2 cut(s) 78, 243
BseBI CCWGG 1 cut(s) 109
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 2 cut(s) 316, 409
BseMI GCAATG 2 cut(s) 78, 243
BseMII CTCAG 1 cut(s) 306
BseRI GAGGAG 1 cut(s) 44
BshFI GGCC 1 cut(s) 396
BshTI ACCGGT 1 cut(s) 284
BsiSI CCGG 2 cut(s) 153, 285
BslI CCNNNNNNNGG 2 cut(s) 316, 409
BsmI GAATGC 1 cut(s) 165
BsnI GGCC 1 cut(s) 396
Bsp143I GATC 2 cut(s) 149, 230
BspACI CCGC 5 cut(s) 11, 14, 105, 156, 423
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 1 cut(s) 307
BspLI GGNNCC 2 cut(s) 151, 212
BspPI GGATC 3 cut(s) 144, 157, 238
BsrDI GCAATG 2 cut(s) 78, 243
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssMI GATC 2 cut(s) 149, 230
BssNI GRCGYC 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 307
BstC8I GCNNGC 2 cut(s) 421, 448
BstDEI CTNAG 2 cut(s) 256, 315
BstF5I GGATG 1 cut(s) 355
BstKTI GATC 2 cut(s) 152, 233
BstMBI GATC 2 cut(s) 149, 230
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 198
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 250
BstYI RGATCY 1 cut(s) 149
BsuRI GGCC 1 cut(s) 396
BtsCI GGATG 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 421, 448
Cfr10I RCCGGY 1 cut(s) 284
CseI GACGC 1 cut(s) 315
Csp6I GTAC 1 cut(s) 112
CspAI ACCGGT 1 cut(s) 284
CviAII CATG 2 cut(s) 261, 451
CviJI RGCY 6 cut(s) 71, 203, 314, 365, 396, 456
CviKI_1 RGCY 6 cut(s) 71, 203, 314, 365, 396, 456
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 2 cut(s) 256, 315
DpnI GATC 2 cut(s) 151, 232
DpnII GATC 2 cut(s) 149, 230
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
EciI GGCGGA 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 107
FaeI CATG 2 cut(s) 264, 454
FaiI YATR 7 cut(s) 262, 276, 339, 452, 459, 483, 490
FatI CATG 2 cut(s) 260, 450
Fnu4HI GCNGC 2 cut(s) 12, 15
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 2 cut(s) 12, 15
GluI GCNGC 2 cut(s) 12, 15
HaeIII GGCC 1 cut(s) 396
HapII CCGG 2 cut(s) 153, 285
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 307
Hin1II CATG 2 cut(s) 264, 454
HinfI GANTC 1 cut(s) 325
HpaII CCGG 2 cut(s) 153, 285
HphI GGTGA 3 cut(s) 31, 94, 280
Hpy188III TCNNGA 1 cut(s) 383
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 2 cut(s) 248, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 2 cut(s) 256, 315
Hsp92I GRCGYC 1 cut(s) 307
Hsp92II CATG 2 cut(s) 264, 454
Kzo9I GATC 2 cut(s) 149, 230
LpnPI CCDG 9 cut(s) 94, 121, 166, 288, 298, 347, 360, 395, 405
LweI GCATC 3 cut(s) 26, 231, 268
MaeIII GTNAC 2 cut(s) 184, 439
MalI GATC 2 cut(s) 151, 232
MboI GATC 2 cut(s) 149, 230
MboII GAAGA 4 cut(s) 28, 91, 135, 398
MflI RGATCY 1 cut(s) 149
MluCI AATT 3 cut(s) 86, 330, 435
MnlI CCTC 6 cut(s) 40, 60, 65, 310, 369, 414
MspI CCGG 2 cut(s) 153, 285
MspR9I CCNGG 1 cut(s) 109
Mva1269I GAATGC 1 cut(s) 165
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 2 cut(s) 149, 230
NlaIII CATG 2 cut(s) 264, 454
NlaIV GGNNCC 2 cut(s) 151, 212
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 439
NspI RCATGY 1 cut(s) 264
PctI GAATGC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 325
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 2 cut(s) 13, 16
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 2 cut(s) 151, 212
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SatI GCNGC 2 cut(s) 12, 15
Sau3AI GATC 2 cut(s) 149, 230
ScrFI CCNGG 1 cut(s) 109
SetI ASST 5 cut(s) 73, 99, 316, 321, 414
SfaNI GCATC 3 cut(s) 26, 231, 268
SfcI CTRYAG 1 cut(s) 198
Sse9I AATT 3 cut(s) 86, 330, 435
SsiI CCGC 5 cut(s) 11, 14, 105, 156, 423
StyD4I CCNGG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 382
TasI AATT 3 cut(s) 86, 330, 435
TauI GCSGC 2 cut(s) 14, 17
TfiI GAWTC 1 cut(s) 325
TseFI GTSAC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 2 cut(s) 79, 354
XceI RCATGY 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.