Rw4G026470

membrane protein YuiD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010674
Physical Location & Seq
Reverse (-)
52681414 .. 52683163
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026470.1

Sequence Viewer

Length: 489 bp
ATGGGTGAGGTGGCGGCAGCTTCATCGACCTCAATCTTCGCCAACTACCCTCTTCTCTCTTCTCTAATCGCTTTCGCAACAGCCCAGCTCATCAAGCTCTTCACCACCTGGTACAAGGAAAGAAGATGGGATATTAAGCAACTTATTGGATCCGGCGGAATGCCATCATCTCATTCTGCGACTGTTACTGCTATTGCTGCAGCCATTGGGTTTCAAGAGGGCGTTGGTGGATCACTCTTTGCAATTGCACTGATCTTAGCATGCGTTGTGATGTATGATGCAACTGGTGTAAGATTACAGGCTGGACGCCAAGCAGAGGTTCTGAATCAAATTGTGTATGAACTTCCTTCTGAGCATCCTCTGGCTGAGAGCAGACCACTGCGTGAACTTCTTGGCCACACACCCCCTCAGGTTATTGCTGGTGGAATTCTTGGAATTGTCACAGCGACCATTGGCCATTTTATAATCTTGGCTGCTAGTCATACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

162

Amino Acids

17.07

Weight (kDa)

6.5

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 14 - 147 6.8e-51 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24350 AT1G24350 AT1G24350 AT1G24350 AT1G24350 AT1G67600
fragaria_vesca FvH4_4g24302
malus_domestica MD13G1113100.v1.1 MD16G1112000.v1.1
prunus_persica Prupe.1G233000_v2.0.a1
pyrus_communis pycom13g09850 pycom16g09500
rosa_chinensis RchiOBHm_Chr4g0431101
rosa_laevigata RLG00000006925
rosa_multiflora Rmu_co8474717.1_g000001 Rmu_sc0010203.1_g000012
rosa_roxburghii Rroxscaffold_5G00372690
rosa_rugosa Rorug04G0248000
rosa_samantha Rh4DG308400
rosa_wichuraiana Rw4G026470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
AciI CCGC 2 cut(s) 14, 156
AclWI GGATC 3 cut(s) 144, 157, 238
AcoI YGGCCR 2 cut(s) 394, 454
AcsI RAATTY 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 307
AdeI CACNNNGTG 1 cut(s) 383
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 1 cut(s) 215
AjnI CCWGG 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 303, 335
AluBI AGCT 3 cut(s) 20, 88, 97
AluI AGCT 3 cut(s) 20, 88, 97
AlwI GGATC 3 cut(s) 144, 157, 238
AoxI GGCC 2 cut(s) 394, 454
ApeKI GCWGC 4 cut(s) 17, 197, 200, 473
ApoI RAATTY 1 cut(s) 426
AsuHPI GGTGA 2 cut(s) 17, 94
AxyI CCTNAGG 1 cut(s) 408
BalI TGGCCA 2 cut(s) 396, 456
BamHI GGATCC 1 cut(s) 149
BbvI GCAGC 4 cut(s) 29, 184, 212, 460
BccI CCATC 2 cut(s) 120, 172
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 109
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 1 cut(s) 198
BisI GCNGC 5 cut(s) 15, 18, 198, 201, 474
BlsI GCNGC 5 cut(s) 16, 19, 199, 202, 475
Bme1390I CCNGG 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 2 cut(s) 268, 364
BsaAI YACGTR 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 307
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 289
Bse21I CCTNAGG 1 cut(s) 408
BseBI CCWGG 1 cut(s) 109
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 3 cut(s) 342, 357, 422
BseNI ACTGG 1 cut(s) 289
BseXI GCAGC 4 cut(s) 29, 184, 212, 460
BseYI CCCAGC 1 cut(s) 84
BshFI GGCC 2 cut(s) 396, 456
BsiSI CCGG 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 316
BsmI GAATGC 1 cut(s) 165
BsnI GGCC 2 cut(s) 396, 456
Bsp143I GATC 3 cut(s) 149, 230, 252
BspACI CCGC 2 cut(s) 14, 156
BspANI GGCC 2 cut(s) 396, 456
BspCNI CTCAG 3 cut(s) 343, 358, 421
BspLI GGNNCC 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 3 cut(s) 144, 157, 238
BspQI GCTCTTC 1 cut(s) 104
BsrI ACTGG 1 cut(s) 289
BssMI GATC 3 cut(s) 149, 230, 252
BssNI GRCGYC 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 3 cut(s) 57, 64, 104
BstACI GRCGYC 1 cut(s) 307
BstBAI YACGTR 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 4 cut(s) 256, 351, 366, 408
BstF5I GGATG 1 cut(s) 355
BstKTI GATC 3 cut(s) 152, 233, 255
BstMBI GATC 3 cut(s) 149, 230, 252
BstMWI GCNNNNNNNGC 2 cut(s) 94, 197
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 4 cut(s) 29, 184, 212, 460
BstX2I RGATCY 1 cut(s) 149
BstYI RGATCY 1 cut(s) 149
Bsu36I CCTNAGG 1 cut(s) 408
BsuRI GGCC 2 cut(s) 396, 456
BtsCI GGATG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 2 cut(s) 248, 377
Cac8I GCNNGC 1 cut(s) 262
CseI GACGC 1 cut(s) 315
CsiI ACCWGGT 1 cut(s) 107
Csp6I GTAC 1 cut(s) 112
CviAII CATG 1 cut(s) 261
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 4 cut(s) 256, 351, 366, 408
DpnI GATC 3 cut(s) 151, 232, 254
DpnII GATC 3 cut(s) 149, 230, 252
DraIII CACNNNGTG 1 cut(s) 383
EaeI YGGCCR 2 cut(s) 394, 454
Eam1104I CTCTTC 3 cut(s) 57, 64, 104
EarI CTCTTC 3 cut(s) 57, 64, 104
EciI GGCGGA 1 cut(s) 171
Eco81I CCTNAGG 1 cut(s) 408
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 107
FaeI CATG 1 cut(s) 264
FaiI YATR 5 cut(s) 262, 276, 339, 464, 483
FatI CATG 1 cut(s) 260
Fnu4HI GCNGC 5 cut(s) 15, 18, 198, 201, 474
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 5 cut(s) 15, 18, 198, 201, 474
FspBI CTAG 1 cut(s) 477
GluI GCNGC 5 cut(s) 15, 18, 198, 201, 474
GsaI CCCAGC 1 cut(s) 88
HaeIII GGCC 2 cut(s) 396, 456
HapII CCGG 1 cut(s) 153
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 307
Hin1II CATG 1 cut(s) 264
HinfI GANTC 1 cut(s) 325
HpaII CCGG 1 cut(s) 153
HphI GGTGA 2 cut(s) 17, 94
Hpy166II GTNNAC 1 cut(s) 386
Hpy188I TCNGA 2 cut(s) 324, 352
Hpy188III TCNNGA 1 cut(s) 215
Hpy8I GTNNAC 1 cut(s) 386
HpyAV CCTTC 1 cut(s) 357
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 4 cut(s) 200, 242, 248, 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 197
HpyF3I CTNAG 4 cut(s) 256, 351, 366, 408
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 307
Hsp92II CATG 1 cut(s) 264
Kzo9I GATC 3 cut(s) 149, 230, 252
LguI GCTCTTC 1 cut(s) 104
Lsp1109I GCAGC 4 cut(s) 29, 184, 212, 460
LweI GCATC 2 cut(s) 268, 364
MabI ACCWGGT 1 cut(s) 107
MaeI CTAG 1 cut(s) 477
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 2 cut(s) 184, 439
MalI GATC 3 cut(s) 151, 232, 254
MboI GATC 3 cut(s) 149, 230, 252
MboII GAAGA 5 cut(s) 28, 44, 51, 91, 135
MfeI CAATTG 1 cut(s) 243
MflI RGATCY 1 cut(s) 149
MlsI TGGCCA 2 cut(s) 396, 456
MluCI AATT 4 cut(s) 243, 330, 426, 435
MluNI TGGCCA 2 cut(s) 396, 456
MnlI CCTC 6 cut(s) 40, 60, 211, 310, 369, 417
Mox20I TGGCCA 2 cut(s) 396, 456
MscI TGGCCA 2 cut(s) 396, 456
MseI TTAA 1 cut(s) 135
Msp20I TGGCCA 2 cut(s) 396, 456
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 1 cut(s) 109
MunI CAATTG 1 cut(s) 243
Mva1269I GAATGC 1 cut(s) 165
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 94, 197
NdeII GATC 3 cut(s) 149, 230, 252
NlaIII CATG 1 cut(s) 264
NlaIV GGNNCC 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 439
NspI RCATGY 1 cut(s) 264
PaeI GCATGC 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 104
PctI GAATGC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 325
PkrI GCNGC 5 cut(s) 16, 19, 199, 202, 475
Ppu21I YACGTR 1 cut(s) 486
PsiI TTATAA 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 107
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 151
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 5 cut(s) 15, 18, 198, 201, 474
Sau3AI GATC 3 cut(s) 149, 230, 252
ScrFI CCNGG 1 cut(s) 109
SetI ASST 9 cut(s) 12, 22, 32, 90, 99, 110, 321, 414, 488
SexAI ACCWGGT 1 cut(s) 107
SfaNI GCATC 2 cut(s) 268, 364
SfcI CTRYAG 1 cut(s) 198
SphI GCATGC 1 cut(s) 264
Sse9I AATT 4 cut(s) 243, 330, 426, 435
SsiI CCGC 2 cut(s) 14, 156
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 488
TaqI TCGA 1 cut(s) 26
TasI AATT 4 cut(s) 243, 330, 426, 435
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 2 cut(s) 255, 384
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 4 cut(s) 17, 197, 200, 473
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 2 cut(s) 12, 354
TspRI CASTG 2 cut(s) 255, 384
XapI RAATTY 1 cut(s) 426
XceI RCATGY 1 cut(s) 264
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.