Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013526

Physical Location & Seq

Genomic Coordinates Reverse (-)

9480657 .. 9483875

Gene Structure 3219 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1130000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1173 bp

ATGGATAGAAGAAGAAGTACCTCAGTAATGAACAGTCCAATCCCAAGGCTTCTCCTTTTGGTATTGGGTTTGTGCTGCCGGCCAGAGGCTGTGCGGTCCCAAGAAATCGAGCCTCCGTCGCCAGTGAAGCATCCCCACTCGAAGAACGAGCACCAAAGAATTATTCTGAGCATTGTGTTGGGAGTCCTGACCGGAGTAATTTGTGCCATCCTCACTGCACTCTTAGTCCGGTGCTTTGTCCGCTACATCAGCAGAACACCAATTCTCAAGGGCCCCGTCATTTTCTCCCCGAAGATCGACCCCAAAACGCTCCAATTAGCACTGGCTAGCGAAAACCAGTTGCTGGGTTCTAGCCCCATTGGGAAGTACTGCAGGACTGTTTTGGACAATGGGCTGATTATTGCAGTCAAGCAGCTCGGACCCTTTTCGGAGTGTGGCTCGCCGGAGGCTCAGAGCAAGGCGGCGAAGCGGCGGATACAGCAGGAGCTTGAAGTGCTTGCTGGCCTAAGACACAGGCATTTGATGAGCCTGCGGGCTTATGTTCGCGAACACGATAGGTTCTCTTTGGTTTATGATTTTGTGCCGAATGGGAGCTTGGAGGATGCTATGAACAGAGTTAGAGAAACCGAGTTGCAGCTCAGTTGGGAAGTTCGGCTTCGGATTGCAGTCGGGGTGATTAAGGGACTTCAGTATCTGCATTCGTATGTTCCTCAGATTATGCACTACAATTTGAAGCCTACAAATGTTATGTTGGATTCGGAGTTTGAGCCGAGGTTGGGAGATTATGGGTTGGCGAAGCTTGCGCCTTATTTGGATGTAGCAACATCTGAATACAGCGCTCCAGAGTGTTTCCAGAACGGCAGGTACACGGATAAGAGCGACATTTTCAGCTTTGGGATGATATTGGGCGTTTTATTAACCGGAAGAGACCCAACCGATCCCTTCTTTGGGGAAGCAGCTAGTGGCGGAAGTTTAGGGCGGTGGCTGCGGCACTTGCAGCAAGCAGGCGAGGCACGGGAAGCATTAGATAAGAGTATAATAGGTGAAGAAGTGGAGGAAGATGAGATGTTGATGGCAGTGCGAATCGCCGTTGTATGCCTATCAGATTTGCCAGCAGAGAGGCCCTCAAGCGATGAACTTGTTCACATGCTAACCCAACTGCACAGTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009888 GO:0009909 GO:0010073 GO:0010075 GO:0016020 GO:0032501 GO:0032502 GO:0033612 GO:0040008 GO:0044464 GO:0048507 GO:0048509 GO:0048580 GO:0048638 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:0071944 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

391

Amino Acids

43.59

Weight (kDa)

6.84

Isoelectric Point (pI)

55.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	113 - 313	3.3e-31	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	149 - 329	2.1e-23	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013526)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13290 AT5G13290 AT5G13290
fragaria_vesca	FvH4_4g22780
malus_domestica	MD13G1129700.v1.1 MD16G1130000.v1.1
prunus_persica	Prupe.1G216600_v2.0.a1 Prupe.1G216600_v2.0.a1
pyrus_communis	pycom16g11060
rosa_chinensis	RchiOBHm_Chr4g0429001
rosa_laevigata	RLG00000007105
rosa_multiflora	Rmu_sc0008629.1_g000015 Rmu_sc0008629.1_g000016
rosa_roxburghii	Rroxscaffold_5G00370660
rosa_rugosa	Rorug04G0231900
rosa_samantha	Rh4BG294100 Rh4CG309600 Rh4DG291000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	852
AccB7I	CCANNNNNTGG	1 cut(s)	343
AccII	CGCG	1 cut(s)	546
AciI	CCGC	9 cut(s)	94, 241, 461, 469, 472, 532, 966, 979, 988
AclWI	GGATC	1 cut(s)	932
AcoI	YGGCCR	1 cut(s)	80
AcuI	CTGAAG	1 cut(s)	671
AfaI	GTAC	3 cut(s)	19, 368, 866
AfeI	AGCGCT	1 cut(s)	838
AfiI	CCNNNNNNNGG	5 cut(s)	85, 343, 776, 947, 948
AgsI	TTSAA	2 cut(s)	491, 733
AjuI	GAANNNNNNNTTGG	4 cut(s)	147, 179, 578, 610
AluBI	AGCT	7 cut(s)	415, 487, 594, 637, 799, 891, 959
AluI	AGCT	7 cut(s)	415, 487, 594, 637, 799, 891, 959
Alw21I	GWGCWC	1 cut(s)	153
Alw26I	GTCTC	1 cut(s)	921
AlwI	GGATC	1 cut(s)	932
AlwNI	CAGNNNCTG	3 cut(s)	89, 343, 694
Aor51HI	AGCGCT	1 cut(s)	838
AoxI	GGCC	4 cut(s)	80, 271, 502, 1121
ApaI	GGGCCC	1 cut(s)	275
ApeKI	GCWGC	6 cut(s)	75, 412, 634, 956, 985, 997
Asp700I	GAANNNNTTC	1 cut(s)	1140
AspLEI	GCGC	2 cut(s)	805, 839
AspS9I	GGNCC	5 cut(s)	96, 271, 272, 419, 1122
AsuHPI	GGTGA	2 cut(s)	685, 1055
AsuNHI	GCTAGC	1 cut(s)	326
AvaII	GGWCC	2 cut(s)	96, 419
BaeGI	GKGCMC	1 cut(s)	275
BaeI	ACNNNNGTAYC	2 cut(s)	674, 707
BanII	GRGCYC	1 cut(s)	275
BarI	GAAGNNNNNNTAC	1 cut(s)	33
Bbv12I	GWGCWC	1 cut(s)	153
BbvI	GCAGC	6 cut(s)	62, 424, 646, 968, 972, 1009
BccI	CCATC	2 cut(s)	215, 1066
BceAI	ACGGC	2 cut(s)	874, 1073
BciVI	GTATCC	1 cut(s)	468
BcoDI	GTCTC	1 cut(s)	921
BfaI	CTAG	3 cut(s)	327, 351, 960
BfmI	CTRYAG	1 cut(s)	370
BfoI	RGCGCY	1 cut(s)	840
BfuAI	ACCTGC	1 cut(s)	852
BfuI	GTATCC	1 cut(s)	468
BisI	GCNGC	9 cut(s)	76, 413, 462, 470, 635, 957, 986, 989, 998
BlsI	GCNGC	9 cut(s)	77, 414, 463, 471, 636, 958, 987, 990, 999
BmcAI	AGTACT	1 cut(s)	368
Bme18I	GGWCC	2 cut(s)	96, 419
BmgT120I	GGNCC	5 cut(s)	96, 271, 272, 419, 1122
BmiI	GGNNCC	4 cut(s)	98, 273, 274, 421
BmsI	GCATC	2 cut(s)	139, 592
BmtI	GCTAGC	1 cut(s)	330
BplI	GAGNNNNNCTC	4 cut(s)	422, 454, 1109, 1141
BpmI	CTGGAG	1 cut(s)	825
BpuEI	CTTGAG	2 cut(s)	251, 1111
BsaI	GGTCTC	1 cut(s)	921
BsaJI	CCNNGG	2 cut(s)	44, 770
BsaWI	WCCGGW	3 cut(s)	191, 228, 920
Bsc4I	CCNNNNNNNGG	5 cut(s)	85, 343, 776, 947, 948
Bse118I	RCCGGY	1 cut(s)	78
Bse1I	ACTGG	3 cut(s)	122, 327, 337
BseDI	CCNNGG	2 cut(s)	44, 770
BseGI	GGATG	5 cut(s)	130, 207, 607, 820, 903
BseLI	CCNNNNNNNGG	5 cut(s)	85, 343, 776, 947, 948
BseMII	CTCAG	5 cut(s)	36, 158, 464, 652, 725
BseNI	ACTGG	3 cut(s)	122, 327, 337
BseSI	GKGCMC	1 cut(s)	275
BseXI	GCAGC	6 cut(s)	62, 424, 646, 968, 972, 1009
BseYI	CCCAGC	1 cut(s)	343
BsgI	GTGCAG	2 cut(s)	201, 1145
Bsh1236I	CGCG	1 cut(s)	546
BshFI	GGCC	4 cut(s)	82, 273, 504, 1123
BsiHKAI	GWGCWC	1 cut(s)	153
BsiSI	CCGG	5 cut(s)	79, 192, 229, 443, 921
BslFI	GGGAC	2 cut(s)	82, 696
BslI	CCNNNNNNNGG	5 cut(s)	85, 343, 776, 947, 948
BsmAI	GTCTC	1 cut(s)	921
BsmFI	GGGAC	2 cut(s)	82, 696
BsmI	GAATGC	1 cut(s)	697
BsnI	GGCC	4 cut(s)	82, 273, 504, 1123
Bso31I	GGTCTC	1 cut(s)	921
Bsp120I	GGGCCC	1 cut(s)	271
Bsp1286I	GDGCHC	2 cut(s)	153, 275
Bsp143I	GATC	2 cut(s)	294, 937
Bsp68I	TCGCGA	1 cut(s)	546
BspACI	CCGC	9 cut(s)	94, 241, 461, 469, 472, 532, 966, 979, 988
BspANI	GGCC	4 cut(s)	82, 273, 504, 1123
BspCNI	CTCAG	5 cut(s)	35, 159, 463, 651, 724
BspFNI	CGCG	1 cut(s)	546
BspLI	GGNNCC	4 cut(s)	98, 273, 274, 421
BspMAI	CTGCAG	1 cut(s)	374
BspMI	ACCTGC	1 cut(s)	852
BspOI	GCTAGC	1 cut(s)	330
BspPI	GGATC	1 cut(s)	932
BspTNI	GGTCTC	1 cut(s)	921
BsrFI	RCCGGY	1 cut(s)	78
BsrI	ACTGG	3 cut(s)	122, 327, 337
BssAI	RCCGGY	1 cut(s)	78
BssECI	CCNNGG	2 cut(s)	44, 770
BssMI	GATC	2 cut(s)	294, 937
BssT1I	CCWWGG	1 cut(s)	44
Bst4CI	ACNGT	3 cut(s)	35, 379, 1166
Bst6I	CTCTTC	1 cut(s)	919
BstDEI	CTNAG	7 cut(s)	22, 167, 223, 450, 506, 638, 711
BstF5I	GGATG	5 cut(s)	130, 207, 607, 820, 903
BstFNI	CGCG	1 cut(s)	546
BstH2I	RGCGCY	1 cut(s)	840
BstHHI	GCGC	2 cut(s)	805, 839
BstKTI	GATC	2 cut(s)	297, 940
BstMAI	GTCTC	1 cut(s)	921
BstMBI	GATC	2 cut(s)	294, 937
BstNSI	RCATGY	1 cut(s)	1150
BstSFI	CTRYAG	1 cut(s)	370
BstSLI	GKGCMC	1 cut(s)	275
BstUI	CGCG	1 cut(s)	546
BstV1I	GCAGC	6 cut(s)	62, 424, 646, 968, 972, 1009
BsuI	GTATCC	1 cut(s)	468
BsuRI	GGCC	4 cut(s)	82, 273, 504, 1123
BtgZI	GCGATG	1 cut(s)	1146
BtsCI	GGATG	5 cut(s)	130, 207, 607, 820, 903
BtsI	GCAGTG	2 cut(s)	213, 1083
BtsIMutI	CAGTG	4 cut(s)	129, 213, 320, 1083
BtuMI	TCGCGA	1 cut(s)	546
BveI	ACCTGC	1 cut(s)	852
CaiI	CAGNNNCTG	3 cut(s)	89, 343, 694
CfoI	GCGC	2 cut(s)	805, 839
Cfr10I	RCCGGY	1 cut(s)	78
Cfr13I	GGNCC	5 cut(s)	96, 271, 272, 419, 1122
Csp6I	GTAC	3 cut(s)	18, 367, 865
CviAII	CATG	1 cut(s)	1147
CviQI	GTAC	3 cut(s)	18, 367, 865
DdeI	CTNAG	7 cut(s)	22, 167, 223, 450, 506, 638, 711
DpnI	GATC	2 cut(s)	296, 939
DpnII	GATC	2 cut(s)	294, 937
EaeI	YGGCCR	1 cut(s)	80
Eam1104I	CTCTTC	1 cut(s)	919
EarI	CTCTTC	1 cut(s)	919
EciI	GGCGGA	2 cut(s)	487, 981
Eco130I	CCWWGG	1 cut(s)	44
Eco24I	GRGCYC	1 cut(s)	275
Eco31I	GGTCTC	1 cut(s)	921
Eco47I	GGWCC	2 cut(s)	96, 419
Eco47III	AGCGCT	1 cut(s)	838
Eco57I	CTGAAG	1 cut(s)	671
EcoO109I	RGGNCCY	3 cut(s)	271, 272, 1122
EcoT14I	CCWWGG	1 cut(s)	44
EcoT38I	GRGCYC	1 cut(s)	275
ErhI	CCWWGG	1 cut(s)	44
FaeI	CATG	1 cut(s)	1150
FalI	AAGNNNNNCTT	2 cut(s)	639, 671
FaqI	GGGAC	2 cut(s)	82, 696
FatI	CATG	1 cut(s)	1146
FauI	CCCGC	1 cut(s)	525
Fnu4HI	GCNGC	9 cut(s)	76, 413, 462, 470, 635, 957, 986, 989, 998
FokI	GGATG	5 cut(s)	117, 194, 614, 827, 910
FriOI	GRGCYC	1 cut(s)	275
Fsp4HI	GCNGC	9 cut(s)	76, 413, 462, 470, 635, 957, 986, 989, 998
FspBI	CTAG	3 cut(s)	327, 351, 960
GlaI	GCGC	2 cut(s)	804, 838
GluI	GCNGC	9 cut(s)	76, 413, 462, 470, 635, 957, 986, 989, 998
GsaI	CCCAGC	1 cut(s)	347
GsuI	CTGGAG	1 cut(s)	825
HaeII	RGCGCY	1 cut(s)	840
HaeIII	GGCC	4 cut(s)	82, 273, 504, 1123
HapII	CCGG	5 cut(s)	79, 192, 229, 443, 921
HhaI	GCGC	2 cut(s)	805, 839
Hin1II	CATG	1 cut(s)	1150
Hin6I	GCGC	2 cut(s)	803, 837
HinP1I	GCGC	2 cut(s)	803, 837
HindIII	AAGCTT	1 cut(s)	797
HinfI	GANTC	3 cut(s)	183, 755, 1083
HpaII	CCGG	5 cut(s)	79, 192, 229, 443, 921
HphI	GGTGA	2 cut(s)	685, 1055
Hpy166II	GTNNAC	2 cut(s)	867, 1144
Hpy188I	TCNGA	9 cut(s)	168, 419, 430, 453, 660, 714, 760, 829, 1105
Hpy188III	TCNNGA	4 cut(s)	187, 545, 842, 853
Hpy8I	GTNNAC	2 cut(s)	867, 1144
Hpy99I	CGWCG	1 cut(s)	121
HpyAV	CCTTC	1 cut(s)	952
HpyCH4III	ACNGT	3 cut(s)	35, 379, 1166
HpyCH4V	TGCA	9 cut(s)	218, 372, 404, 634, 665, 697, 721, 997, 1162
HpyF3I	CTNAG	7 cut(s)	22, 167, 223, 450, 506, 638, 711
Hsp92II	CATG	1 cut(s)	1150
HspAI	GCGC	2 cut(s)	803, 837
KroI	GCCGGC	1 cut(s)	78
KroNI	GCCGGC	1 cut(s)	80
Kzo9I	GATC	2 cut(s)	294, 937
LmnI	GCTCC	4 cut(s)	315, 484, 591, 844
Lsp1109I	GCAGC	6 cut(s)	62, 424, 646, 968, 972, 1009
LweI	GCATC	2 cut(s)	139, 592
MaeI	CTAG	3 cut(s)	327, 351, 960
MalI	GATC	2 cut(s)	296, 939
MboI	GATC	2 cut(s)	294, 937
MboII	GAAGA	7 cut(s)	21, 24, 154, 304, 936, 1058, 1070
MhlI	GDGCHC	2 cut(s)	153, 275
MluCI	AATT	5 cut(s)	159, 198, 261, 314, 727
MlyI	GAGTC	1 cut(s)	192
MmeI	TCCRAC	1 cut(s)	732
MroNI	GCCGGC	1 cut(s)	78
MroXI	GAANNNNTTC	1 cut(s)	1140
MseI	TTAA	2 cut(s)	678, 917
MslI	CAYNNNNRTG	1 cut(s)	702
MspI	CCGG	5 cut(s)	79, 192, 229, 443, 921
Mva1269I	GAATGC	1 cut(s)	697
MvnI	CGCG	1 cut(s)	546
NaeI	GCCGGC	1 cut(s)	80
NdeII	GATC	2 cut(s)	294, 937
NgoMIV	GCCGGC	1 cut(s)	78
NheI	GCTAGC	1 cut(s)	326
NlaIII	CATG	1 cut(s)	1150
NlaIV	GGNNCC	4 cut(s)	98, 273, 274, 421
NmeAIII	GCCGAG	1 cut(s)	795
NruI	TCGCGA	1 cut(s)	546
NspI	RCATGY	1 cut(s)	1150
PctI	GAATGC	1 cut(s)	697
PdiI	GCCGGC	1 cut(s)	80
PdmI	GAANNNNTTC	1 cut(s)	1140
PfeI	GAWTC	2 cut(s)	755, 1083
PflMI	CCANNNNNTGG	1 cut(s)	343
PkrI	GCNGC	9 cut(s)	77, 414, 463, 471, 636, 958, 987, 990, 999
PleI	GAGTC	1 cut(s)	191
PpsI	GAGTC	1 cut(s)	191
PspFI	CCCAGC	1 cut(s)	343
PspN4I	GGNNCC	4 cut(s)	98, 273, 274, 421
PspOMI	GGGCCC	1 cut(s)	271
PspPI	GGNCC	5 cut(s)	96, 271, 272, 419, 1122
PsrI	GAACNNNNNNTAC	2 cut(s)	848, 880
PstI	CTGCAG	1 cut(s)	374
PstNI	CAGNNNCTG	3 cut(s)	89, 343, 694
RruI	TCGCGA	1 cut(s)	546
RsaI	GTAC	3 cut(s)	19, 368, 866
RsaNI	GTAC	3 cut(s)	18, 367, 865
RseI	CAYNNNNRTG	1 cut(s)	702
SaqAI	TTAA	2 cut(s)	678, 917
SatI	GCNGC	9 cut(s)	76, 413, 462, 470, 635, 957, 986, 989, 998
Sau3AI	GATC	2 cut(s)	294, 937
Sau96I	GGNCC	5 cut(s)	96, 271, 272, 419, 1122
ScaI	AGTACT	1 cut(s)	368
SchI	GAGTC	1 cut(s)	192
SduI	GDGCHC	2 cut(s)	153, 275
SfaNI	GCATC	2 cut(s)	139, 592
SfcI	CTRYAG	1 cut(s)	370
SinI	GGWCC	2 cut(s)	96, 419
SmiMI	CAYNNNNRTG	1 cut(s)	702
SmlI	CTYRAG	2 cut(s)	266, 1126
SmoI	CTYRAG	2 cut(s)	266, 1126
Sse9I	AATT	5 cut(s)	159, 198, 261, 314, 727
SsiI	CCGC	9 cut(s)	94, 241, 461, 469, 472, 532, 966, 979, 988
SspMI	CTAG	3 cut(s)	327, 351, 960
StyI	CCWWGG	1 cut(s)	44
TaaI	ACNGT	3 cut(s)	35, 379, 1166
TaqI	TCGA	3 cut(s)	108, 140, 297
TasI	AATT	5 cut(s)	159, 198, 261, 314, 727
TatI	WGTACW	1 cut(s)	366
TauI	GCSGC	3 cut(s)	464, 472, 991
TfiI	GAWTC	2 cut(s)	755, 1083
Tru1I	TTAA	2 cut(s)	678, 917
Tru9I	TTAA	2 cut(s)	678, 917
TscAI	CASTG	4 cut(s)	129, 220, 327, 1083
TseI	GCWGC	6 cut(s)	75, 412, 634, 956, 985, 997
TspDTI	ATGAA	3 cut(s)	44, 623, 1149
TspGWI	ACGGA	2 cut(s)	105, 884
TspRI	CASTG	4 cut(s)	129, 220, 327, 1083
Van91I	CCANNNNNTGG	1 cut(s)	343
VpaK11BI	GGWCC	2 cut(s)	96, 419
XceI	RCATGY	1 cut(s)	1150
XmnI	GAANNNNTTC	1 cut(s)	1140
XspI	CTAG	3 cut(s)	327, 351, 960
ZrmI	AGTACT	1 cut(s)	368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.