MD16G1247100.v1.1

vascular transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011356
Physical Location & Seq
Reverse (-)
27514759 .. 27520269
5511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1247100.v1.1.491

Sequence Viewer

Length: 3156 bp
ATGCGCTCAGGAGGTAGTGCGGTTCAGCAGACCCTCACAGCGGAGGCTGCTTCGGTGTTGAAGCACTCTCTCAACCTCGCAAGGCGGAGAGGCCATGCTCAGGTCACTCCTCTCCACGTGGCCGCCACTCTGCTCAGCTCAAGAACTAGCCTCTTGCGGCGGGCCTGTCTCAAATCTCAGGCGCACCAAACCTCTCATCCTCTCCAATGCCGAGCCCTCGAGCTCTGCTTCAATGTGGCTCTCAACCGTCTCCCAACAACTCCTGGCCCTCTCCTCCACGGCCAGCCCTCTCTCTCCAACGCCCTCATCGCCGCCCTCAAGAGAGCGCAAGCCCACCAGAGAAGAGGCTGCATCGAGCAGCAGCAGCAGCAGCCGCTCTTAACCATCAAAGTGGAGCTCGAGCAGCTCATCATCTCCATCCTCGACGACCCGAGTGTCAGCAGGGTCATGAAAGAGGCTGGTTTCTCCAGCACTAACGTCAAGAACAACCTGGAGGACTCCTCAACTTCCTCCGTTTTCCAGTGTTACAGCAGCTCCAGCGGCGTTTTCTCTTCCCCTTGCTCTCCTTCTCCTCCCTCAGAACACCACCACCAAAACAATATAATTCCTGTGAGTTTCTGGCAACCCCACTTCCTGGCTTACACTGGTGAGCAAAATCCACTTCTTTTTTCCCCTCAAAAGGCAAAACCTCCATTGATAATCAGTAACCCCACCGCCGCCGCTGCCAATGACTGCTCTGGTAGTTGTTCACAGGAAGAAGATGTTAAGTTGGTGTTGGAGGTGCTGGTGAGGAAGAAGAGGAGGAACACTGTGATAGTTGGTGATTCAGCTTCCATGACCGAAGGGCTTGTCTCTGAAGTTATGGGGAGGATAGAGAGAGGAGTGGGAGTTCCTGAGGAGTTGAAATCAACCCAGTTCATAAAGTTCCAATTCTCACCTGTTTCTGTGAGGTTCATGAAAAGAGAAGATGTGGAAGCAAATCTCTCAGAGCTCAAAAGAAAAGTGGACTCATCATTTCTCGGAGAAAGAGGTGCTATAATTTACACCGGAGATCTAAAGTGGGTGATTAGTGATGAGGAGAGAGATCAAAACCAAAACTCTGGTGGGTATTATAGCCCAGTTGAGCATTTGGTTTCAGAGATTGCAAGGTTGGTCTCTTCAGATAATTCAATCACTAGCTCAAGGGGGAAGATGTGGGTGATGGGAACTGCAAGTTACCAGACACACATGAGGTGCCAAATTCGGCAGCCACCTCTTGAGCTTCAATGGTGTCTTCAAGCTGTTTCTGTTCCTTCTGGTGGTCTTGGTCTCTCCCTTCATGCTTCCAGTGTCCAAGACTCAAGATTAATATTCTCTCAAAAACCATTTGAAGTTCTGGAAACAAAACCAATATTCAATAGCAAGGAACAATATCATAATAATAACAAGCTCACTTGCTGTCAAGAATGCACTTCAAATTATGAAAAAGAATCTCAGCTCTTGAAAGCAGACGAGCAGAAATTGCCTGCCTGGCTGCAACCACATGGAACCGAAGCCCGCCAAAAGGATGAAGTAGCTCAATTGAGGAAAAAGTGGAACAGCTTATGCTGCATTCTGCACCAAGGAAGACATGCAGCTCAGAACAATTTGAGCTCTTCTAATTTGTACAACAATCAAAGCGTAATTGGAAACAACTCCTCTTATGCTTCAGCATACCCTTGGTGGTCCACCAAGAATGGCTTCTCTCCAGATCCGAATTCAATCTCCTTCAGTCACGATCCAGCTTCAGAGCTTGCTCACGGCTCTAGTCTCGTGCCTCGATTCAGGAGACAACAATCGTGCATGATTGAGTTCAACTTCGACAATGGGGTCCAAAAGAATCAAGTGATGGAACCAAGTCTGGATTCTCTTAAAAACACTGAAGGCAAGGAACTGAAGAGCACTCTTGCTCTTGGCAACTCTGTGTTTTCAGGCTCGGAGAAAGTGGAGAGAAAATGCACTGAAAGGACAATGCAGAGAGCTGACATGTGCAAGCTACTGAAAGAGAATGTTCCTTGGCAATCCGAATCCATTCCTTCAATTGTAGAAGCGATTTTCAACTTTAAAACTGCCAGGCAGGAGACTTGGTTGCTGGTTCAGGGGAATGACTCGATTGGAAAAAGAAGGCTGGCACATGCAATTGCTGAATTGGTTCTTGGGTCTGCTGATTCACTCCTCCATATTGACATCAACAAAAATGAAAATGAGATGAACCCGAGTGTAGAAGTTGTAGCAAGAGCCTTGAAATCCAATGAGAAACTTGTTGTCTTGGTAGAAGATGTTGATTTGGCTGACACCCAGTTCTTGAAATTTTTAGCTGATGGTTTCAAGAACCGAAGATTTGGAGAAGTTAGTAGAAGTGAAGGGATTCAAAGCCAAGCCATATTCATTTTGGCTAAGAGTGACTCAACAAGATATGATGAAGAGAAGGCCAAGTACCCGGATTCTATAATCCAAATGGCGTTGAAGGTTGATGAGAAAGCTAGTACTAGTCCTAGTTTTCAGGGAGTTAACTTCGATCACAAGAGAAAGGCTGAGTGGGAGCTACAAATCAAGACCAAGAGTTCAAGAAGTGGAGAGAAGGAAGATCCAAGTGTGGTTGCTGTTGAGAATGTGAATAACAAGGAGGTCTTCTCAAGGCAATCAAGCTTCAACACCCTTGATCTCAACCTCAAAGCCGGGGAGGACGATGAAATGGAAGACAAGGCAGGGGAGCTGAGCCCCAATTCAAGTGGCTTAACTCGCGAAACCAACACCAATCTCGAAACCCCACATGGGTTCCTGGAATCGATTGAGAACCTCTTTGTTTTCAATCGGAGCCCGGCTAGAGATCGAGAGGCAACCGAGCTTTTCTTGTCCAAGATTGAAGGGTGTTTTGGGGAGGTATATGGGAAACTAAATGTGGTTAGATTTAGTGTGGATAAGAGGGTGTTAGAGGGGGTTTTTATTGGATCCGGATATTTTCCCAATAGCCTGTTTGAGAAATGGCTGAAAGACATTTTTCAGACAAGCTTGAGGGCGGTTAAACTTAGCGGGAAAGAGGGTATACTTGTAAGGCTCTGTTTGGGTGACATAGAAGATGGCATTTTGGAGGGTTTCTTGGGTTCTTGTCTTCCTAAGAAAATCCATATTTCTTGA

Protein Analysis

1052

Amino Acids

116.94

Weight (kDa)

7.11

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 114 7.9e-08 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 250 - 443 5.9e-81 SMAX1 nucleotide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29920 AT4G29920
fragaria_vesca FvH4_4g07050 FvH4_4g07050
malus_domestica MD00G1086800.v1.1 MD16G1247100.v1.1
prunus_persica Prupe.1G073700_v2.0.a1
pyrus_communis pycom13g21290 pycom16g20740
rosa_chinensis RchiOBHm_Chr4g0399171
rosa_laevigata RLG00000009293
rosa_multiflora Rmu_sc0002062.1_g000001 Rmu_sc0006088.1_g000002
rosa_roxburghii Rroxscaffold_5G00344130
rosa_rugosa Rorug04G0014600
rosa_samantha Rh4AG092500 Rh4BG090500 Rh4CG101000 Rh4DG084900
rosa_wichuraiana Rw4G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 434, 1847
AccB1I GGYRCC 1 cut(s) 1233
AccB7I CCANNNNNTGG 1 cut(s) 634
AccBSI CCGCTC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 3064
AccII CGCG 1 cut(s) 2763
AccIII TCCGGA 1 cut(s) 2972
AclWI GGATC 5 cut(s) 1724, 1751, 2600, 2964, 2977
AcoI YGGCCR 2 cut(s) 120, 280
AcsI RAATTY 3 cut(s) 1239, 1735, 2327
AcuI CTGAAG 7 cut(s) 876, 1143, 1671, 1732, 1749, 1920, 1934
AcvI CACGTG 1 cut(s) 118
AfaI GTAC 3 cut(s) 1646, 2456, 2506
AfiI CCNNNNNNNGG 7 cut(s) 40, 100, 634, 679, 1298, 1543, 2793
AflIII ACRYGT 1 cut(s) 2004
AhlI ACTAGT 1 cut(s) 2507
AjnI CCWGG 6 cut(s) 262, 489, 633, 1508, 2090, 2799
AjuI GAANNNNNNNTTGG 8 cut(s) 1703, 1735, 1867, 1899, 2157, 2189, 2877, 2909
AloI GAACNNNNNNTCC 2 cut(s) 873, 905
Alw21I GWGCWC 5 cut(s) 225, 399, 993, 1634, 1922
Alw26I GTCTC 8 cut(s) 173, 254, 856, 1159, 1313, 1793, 1801, 2093
AlwI GGATC 5 cut(s) 1724, 1751, 2600, 2964, 2977
Ama87I CYCGRG 4 cut(s) 218, 398, 430, 2233
Aor13HI TCCGGA 1 cut(s) 2972
AoxI GGCC 6 cut(s) 91, 120, 162, 265, 280, 2448
ApoI RAATTY 3 cut(s) 1239, 1735, 2327
ArsI GACNNNNNNTTYG 2 cut(s) 834, 866
AseI ATTAAT 1 cut(s) 1346
Asp700I GAANNNNTTC 4 cut(s) 1718, 2049, 2169, 2385
AspLEI GCGC 3 cut(s) 6, 184, 328
AspS9I GGNCC 4 cut(s) 162, 266, 1704, 1849
AsuC2I CCSGG 3 cut(s) 2459, 2698, 2840
AsuHPI GGTGA 7 cut(s) 659, 799, 833, 927, 1075, 1210, 3098
AvaI CYCGRG 4 cut(s) 218, 398, 430, 2233
AvaII GGWCC 2 cut(s) 1704, 1849
AxyI CCTNAGG 1 cut(s) 894
BamHI GGATCC 1 cut(s) 2969
BanI GGYRCC 1 cut(s) 1233
BanII GRGCYC 7 cut(s) 217, 225, 399, 993, 1634, 2741, 2840
BarI GAAGNNNNNNTAC 2 cut(s) 2438, 2470
BauI CACGAG 1 cut(s) 1790
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 5 cut(s) 1265, 1612, 2641, 2724, 3122
Bbv12I GWGCWC 5 cut(s) 225, 399, 993, 1634, 1922
BccI CCATC 6 cut(s) 392, 425, 1195, 1861, 2333, 3092
BceAI ACGGC 2 cut(s) 295, 1795
BciT130I CCWGG 6 cut(s) 264, 491, 635, 1510, 2092, 2801
BcnI CCSGG 3 cut(s) 2459, 2698, 2840
BcoDI GTCTC 8 cut(s) 173, 254, 856, 1159, 1313, 1793, 1801, 2093
BcuI ACTAGT 1 cut(s) 2507
BfaI CTAG 7 cut(s) 147, 1176, 1785, 2502, 2508, 2514, 2844
BglI GCCNNNNNGGC 1 cut(s) 1510
BglII AGATCT 1 cut(s) 1051
BlpI GCTNAGC 2 cut(s) 134, 2735
BmcAI AGTACT 1 cut(s) 2506
Bme1390I CCNGG 9 cut(s) 264, 491, 635, 1510, 2092, 2459, 2698, 2801, 2840
Bme18I GGWCC 2 cut(s) 1704, 1849
BmeT110I CYCGRG 4 cut(s) 218, 398, 430, 2233
BmgT120I GGNCC 4 cut(s) 162, 266, 1704, 1849
BmiI GGNNCC 7 cut(s) 1235, 1528, 1850, 1872, 2798, 2837, 2971
BmrFI CCNGG 9 cut(s) 264, 491, 635, 1510, 2092, 2459, 2698, 2801, 2840
BmrI ACTGGG 3 cut(s) 907, 1112, 2311
BmsI GCATC 1 cut(s) 360
BmuI ACTGGG 3 cut(s) 907, 1112, 2311
BpiI GAAGAC 5 cut(s) 1265, 1612, 2641, 2724, 3122
BplI GAGNNNNNCTC 4 cut(s) 485, 517, 2636, 2668
BpmI CTGGAG 4 cut(s) 451, 512, 520, 1710
Bpu10I CCTNAGC 2 cut(s) 7, 99
Bpu1102I GCTNAGC 2 cut(s) 134, 2735
BpuEI CTTGAG 7 cut(s) 124, 302, 1165, 1277, 1324, 2638, 3052
BpuMI CCSGG 3 cut(s) 2459, 2698, 2840
Bsa29I ATCGAT 1 cut(s) 2807
BsaAI YACGTR 1 cut(s) 118
BsaI GGTCTC 2 cut(s) 1159, 1313
BsaJI CCNNGG 5 cut(s) 277, 1600, 1697, 2033, 2697
BsaWI WCCGGW 2 cut(s) 1046, 2972
BsaXI ACNNNNNCTCC 6 cut(s) 518, 548, 873, 903, 2090, 2120
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 100, 634, 679, 1298, 1543, 2793
Bse1I ACTGG 6 cut(s) 520, 649, 913, 1118, 1326, 2317
Bse21I CCTNAGG 1 cut(s) 894
BseAI TCCGGA 1 cut(s) 2972
BseBI CCWGG 6 cut(s) 264, 491, 635, 1510, 2092, 2801
BseCI ATCGAT 1 cut(s) 2807
BseDI CCNNGG 5 cut(s) 277, 1600, 1697, 2033, 2697
BseGI GGATG 3 cut(s) 196, 417, 1552
BseLI CCNNNNNNNGG 7 cut(s) 40, 100, 634, 679, 1298, 1543, 2793
BseNI ACTGG 6 cut(s) 520, 649, 913, 1118, 1326, 2317
BsgI GTGCAG 1 cut(s) 1580
Bsh1236I CGCG 1 cut(s) 2763
BshFI GGCC 6 cut(s) 93, 122, 164, 267, 282, 2450
BshNI GGYRCC 1 cut(s) 1233
BshVI ATCGAT 1 cut(s) 2807
BsiHKAI GWGCWC 5 cut(s) 225, 399, 993, 1634, 1922
BsiHKCI CYCGRG 4 cut(s) 218, 398, 430, 2233
BsiSI CCGG 5 cut(s) 1047, 2459, 2697, 2840, 2973
BslI CCNNNNNNNGG 7 cut(s) 40, 100, 634, 679, 1298, 1543, 2793
BsmAI GTCTC 8 cut(s) 173, 254, 856, 1159, 1313, 1793, 1801, 2093
BsmBI CGTCTC 1 cut(s) 254
BsmI GAATGC 2 cut(s) 1451, 1590
BsnI GGCC 6 cut(s) 93, 122, 164, 267, 282, 2450
Bso31I GGTCTC 2 cut(s) 1159, 1313
BsoBI CYCGRG 4 cut(s) 218, 398, 430, 2233
Bsp1286I GDGCHC 8 cut(s) 217, 225, 399, 993, 1634, 1922, 2741, 2840
Bsp13I TCCGGA 1 cut(s) 2972
Bsp1407I TGTACA 1 cut(s) 1644
Bsp143I GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
Bsp1720I GCTNAGC 2 cut(s) 134, 2735
Bsp68I TCGCGA 1 cut(s) 2763
BspANI GGCC 6 cut(s) 93, 122, 164, 267, 282, 2450
BspDI ATCGAT 1 cut(s) 2807
BspEI TCCGGA 1 cut(s) 2972
BspFNI CGCG 1 cut(s) 2763
BspHI TCATGA 2 cut(s) 447, 954
BspLI GGNNCC 7 cut(s) 1235, 1528, 1850, 1872, 2798, 2837, 2971
BspPI GGATC 5 cut(s) 1724, 1751, 2600, 2964, 2977
BspQI GCTCTTC 2 cut(s) 1639, 1910
BspT107I GGYRCC 1 cut(s) 1233
BspTNI GGTCTC 2 cut(s) 1159, 1313
BsrBI CCGCTC 1 cut(s) 376
BsrGI TGTACA 1 cut(s) 1644
BsrI ACTGG 6 cut(s) 520, 649, 913, 1118, 1326, 2317
BssECI CCNNGG 5 cut(s) 277, 1600, 1697, 2033, 2697
BssMI GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
BssNAI GTATAC 1 cut(s) 3065
BssSI CACGAG 1 cut(s) 1790
BssT1I CCWWGG 3 cut(s) 1600, 1697, 2033
Bst1107I GTATAC 1 cut(s) 3065
Bst2BI CACGAG 1 cut(s) 1790
Bst2UI CCWGG 6 cut(s) 264, 491, 635, 1510, 2092, 2801
Bst4CI ACNGT 2 cut(s) 248, 811
Bst6I CTCTTC 7 cut(s) 337, 556, 791, 1162, 1639, 1910, 2436
BstAPI GCANNNNNTGC 1 cut(s) 1501
BstAUI TGTACA 1 cut(s) 1644
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 8 cut(s) 162, 284, 330, 1506, 1537, 1773, 2012, 2148
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 3 cut(s) 196, 417, 1552
BstFNI CGCG 1 cut(s) 2763
BstHHI GCGC 3 cut(s) 6, 184, 328
BstKTI GATC 9 cut(s) 1054, 1087, 1732, 1759, 2539, 2608, 2683, 2851, 2972
BstMAI GTCTC 8 cut(s) 173, 254, 856, 1159, 1313, 1793, 1801, 2093
BstMBI GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
BstNI CCWGG 6 cut(s) 264, 491, 635, 1510, 2092, 2801
BstNSI RCATGY 3 cut(s) 1613, 2008, 2156
BstSCI CCNGG 9 cut(s) 262, 489, 633, 1508, 2090, 2457, 2696, 2799, 2838
BstUI CGCG 1 cut(s) 2763
BstV2I GAAGAC 5 cut(s) 1265, 1612, 2641, 2724, 3122
BstX2I RGATCY 4 cut(s) 1051, 1729, 2605, 2969
BstXI CCANNNNNNTGG 2 cut(s) 391, 1100
BstYI RGATCY 4 cut(s) 1051, 1729, 2605, 2969
BstZ17I GTATAC 1 cut(s) 3065
Bsu15I ATCGAT 1 cut(s) 2807
Bsu36I CCTNAGG 1 cut(s) 894
BsuRI GGCC 6 cut(s) 93, 122, 164, 267, 282, 2450
BsuTUI ATCGAT 1 cut(s) 2807
BtgI CCRYGG 1 cut(s) 277
BtgZI GCGATG 1 cut(s) 292
BtsCI GGATG 3 cut(s) 196, 417, 1552
BtsIMutI CAGTG 6 cut(s) 527, 642, 807, 1333, 1896, 1977
BtuMI TCGCGA 1 cut(s) 2763
Cac8I GCNNGC 8 cut(s) 162, 284, 330, 1506, 1537, 1773, 2012, 2148
CciI TCATGA 2 cut(s) 447, 954
CfoI GCGC 3 cut(s) 6, 184, 328
Cfr13I GGNCC 4 cut(s) 162, 266, 1704, 1849
ClaI ATCGAT 1 cut(s) 2807
Csp6I GTAC 3 cut(s) 1645, 2455, 2505
CspCI CAANNNNNGTGG 2 cut(s) 2731, 2766
CviQI GTAC 3 cut(s) 1645, 2455, 2505
DpnI GATC 9 cut(s) 1053, 1086, 1731, 1758, 2538, 2607, 2682, 2850, 2971
DpnII GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
DraI TTTAAA 1 cut(s) 2083
DrdI GACNNNNNNGTC 2 cut(s) 434, 1847
DseDI GACNNNNNNGTC 2 cut(s) 434, 1847
EaeI YGGCCR 2 cut(s) 120, 280
Eam1104I CTCTTC 7 cut(s) 337, 556, 791, 1162, 1639, 1910, 2436
EarI CTCTTC 7 cut(s) 337, 556, 791, 1162, 1639, 1910, 2436
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 4 cut(s) 223, 397, 991, 1632
Eco130I CCWWGG 3 cut(s) 1600, 1697, 2033
Eco24I GRGCYC 7 cut(s) 217, 225, 399, 993, 1634, 2741, 2840
Eco31I GGTCTC 2 cut(s) 1159, 1313
Eco47I GGWCC 2 cut(s) 1704, 1849
Eco53kI GAGCTC 4 cut(s) 223, 397, 991, 1632
Eco57I CTGAAG 7 cut(s) 876, 1143, 1671, 1732, 1749, 1920, 1934
Eco72I CACGTG 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 894
Eco88I CYCGRG 4 cut(s) 218, 398, 430, 2233
EcoICRI GAGCTC 4 cut(s) 223, 397, 991, 1632
EcoRI GAATTC 1 cut(s) 1735
EcoRII CCWGG 6 cut(s) 262, 489, 633, 1508, 2090, 2799
EcoT14I CCWWGG 3 cut(s) 1600, 1697, 2033
EcoT38I GRGCYC 7 cut(s) 217, 225, 399, 993, 1634, 2741, 2840
ErhI CCWWGG 3 cut(s) 1600, 1697, 2033
Esp3I CGTCTC 1 cut(s) 254
FalI AAGNNNNNCTT 4 cut(s) 1703, 1735, 2633, 2665
FauI CCCGC 3 cut(s) 153, 1544, 3044
FblI GTMKAC 1 cut(s) 3064
FokI GGATG 3 cut(s) 183, 404, 1559
FriOI GRGCYC 7 cut(s) 217, 225, 399, 993, 1634, 2741, 2840
FspBI CTAG 7 cut(s) 147, 1176, 1785, 2502, 2508, 2514, 2844
GlaI GCGC 3 cut(s) 5, 183, 327
GsuI CTGGAG 4 cut(s) 451, 512, 520, 1710
HaeIII GGCC 6 cut(s) 93, 122, 164, 267, 282, 2450
HapII CCGG 5 cut(s) 1047, 2459, 2697, 2840, 2973
HhaI GCGC 3 cut(s) 6, 184, 328
Hin6I GCGC 3 cut(s) 4, 182, 326
HinP1I GCGC 3 cut(s) 4, 182, 326
HincII GTYRAC 1 cut(s) 2530
HindII GTYRAC 1 cut(s) 2530
HindIII AAGCTT 2 cut(s) 2665, 3028
HpaI GTTAAC 1 cut(s) 2530
HpaII CCGG 5 cut(s) 1047, 2459, 2697, 2840, 2973
HphI GGTGA 7 cut(s) 659, 799, 833, 927, 1075, 1210, 3098
Hpy166II GTNNAC 5 cut(s) 749, 1006, 1707, 2530, 3065
Hpy8I GTNNAC 5 cut(s) 749, 1006, 1707, 2530, 3065
Hpy99I CGWCG 1 cut(s) 428
HpyCH4III ACNGT 2 cut(s) 248, 811
HpyCH4IV ACGT 2 cut(s) 117, 477
HpySE526I ACGT 2 cut(s) 117, 477
HspAI GCGC 3 cut(s) 4, 182, 326
Kpn2I TCCGGA 1 cut(s) 2972
KspAI GTTAAC 1 cut(s) 2530
Kzo9I GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
LguI GCTCTTC 2 cut(s) 1639, 1910
LmnI GCTCC 5 cut(s) 394, 539, 2560, 2731, 2835
LweI GCATC 1 cut(s) 360
MaeI CTAG 7 cut(s) 147, 1176, 1785, 2502, 2508, 2514, 2844
MaeII ACGT 2 cut(s) 117, 477
MaeIII GTNAC 7 cut(s) 103, 524, 704, 1214, 1751, 2420, 3086
MalI GATC 9 cut(s) 1053, 1086, 1731, 1758, 2538, 2607, 2682, 2850, 2971
MbiI CCGCTC 1 cut(s) 376
MboI GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
MfeI CAATTG 3 cut(s) 1559, 2058, 2157
MflI RGATCY 4 cut(s) 1051, 1729, 2605, 2969
MhlI GDGCHC 8 cut(s) 217, 225, 399, 993, 1634, 1922, 2741, 2840
MlyI GAGTC 5 cut(s) 491, 1001, 1331, 2120, 2417
MmeI TCCRAC 2 cut(s) 321, 756
MroI TCCGGA 1 cut(s) 2972
MroXI GAANNNNTTC 4 cut(s) 1718, 2049, 2169, 2385
MseI TTAA 8 cut(s) 380, 765, 1346, 1890, 2082, 2529, 2756, 3042
MslI CAYNNNNRTG 1 cut(s) 389
MspA1I CMGCKG 3 cut(s) 41, 540, 722
MspI CCGG 5 cut(s) 1047, 2459, 2697, 2840, 2973
MspR9I CCNGG 9 cut(s) 264, 491, 635, 1510, 2092, 2459, 2698, 2801, 2840
MunI CAATTG 3 cut(s) 1559, 2058, 2157
Mva1269I GAATGC 2 cut(s) 1451, 1590
MvaI CCWGG 6 cut(s) 264, 491, 635, 1510, 2092, 2801
MvnI CGCG 1 cut(s) 2763
NciI CCSGG 3 cut(s) 2459, 2698, 2840
NdeII GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
NlaIV GGNNCC 7 cut(s) 1235, 1528, 1850, 1872, 2798, 2837, 2971
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 4 cut(s) 103, 1751, 2420, 3086
NruI TCGCGA 1 cut(s) 2763
NspI RCATGY 3 cut(s) 1613, 2008, 2156
PaeR7I CTCGAG 2 cut(s) 218, 398
PagI TCATGA 2 cut(s) 447, 954
PciI ACATGT 1 cut(s) 2004
PciSI GCTCTTC 2 cut(s) 1639, 1910
PctI GAATGC 2 cut(s) 1451, 1590
PdmI GAANNNNTTC 4 cut(s) 1718, 2049, 2169, 2385
PflMI CCANNNNNTGG 1 cut(s) 634
PfoI TCCNGGA 1 cut(s) 2799
PleI GAGTC 5 cut(s) 491, 1001, 1331, 2120, 2417
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 5 cut(s) 491, 1001, 1331, 2120, 2417
Ppu21I YACGTR 1 cut(s) 118
PscI ACATGT 1 cut(s) 2004
PshBI ATTAAT 1 cut(s) 1346
Psp124BI GAGCTC 4 cut(s) 225, 399, 993, 1634
Psp6I CCWGG 6 cut(s) 262, 489, 633, 1508, 2090, 2799
PspCI CACGTG 1 cut(s) 118
PspGI CCWGG 6 cut(s) 262, 489, 633, 1508, 2090, 2799
PspN4I GGNNCC 7 cut(s) 1235, 1528, 1850, 1872, 2798, 2837, 2971
PspPI GGNCC 4 cut(s) 162, 266, 1704, 1849
PspXI VCTCGAGB 2 cut(s) 218, 398
PsuI RGATCY 4 cut(s) 1051, 1729, 2605, 2969
RruI TCGCGA 1 cut(s) 2763
RsaI GTAC 3 cut(s) 1646, 2456, 2506
RsaNI GTAC 3 cut(s) 1645, 2455, 2505
RseI CAYNNNNRTG 1 cut(s) 389
SacI GAGCTC 4 cut(s) 225, 399, 993, 1634
SapI GCTCTTC 2 cut(s) 1639, 1910
SaqAI TTAA 8 cut(s) 380, 765, 1346, 1890, 2082, 2529, 2756, 3042
Sau3AI GATC 9 cut(s) 1051, 1084, 1729, 1756, 2536, 2605, 2680, 2848, 2969
Sau96I GGNCC 4 cut(s) 162, 266, 1704, 1849
ScaI AGTACT 1 cut(s) 2506
SchI GAGTC 5 cut(s) 491, 1001, 1331, 2120, 2417
ScrFI CCNGG 9 cut(s) 264, 491, 635, 1510, 2092, 2459, 2698, 2801, 2840
SduI GDGCHC 8 cut(s) 217, 225, 399, 993, 1634, 1922, 2741, 2840
SfaNI GCATC 1 cut(s) 360
Sfr274I CTCGAG 2 cut(s) 218, 398
SinI GGWCC 2 cut(s) 1704, 1849
SlaI CTCGAG 2 cut(s) 218, 398
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 9 cut(s) 139, 218, 317, 398, 1180, 1256, 1339, 2653, 3031
SmoI CTYRAG 9 cut(s) 139, 218, 317, 398, 1180, 1256, 1339, 2653, 3031
SpeI ACTAGT 1 cut(s) 2507
SspI AATATT 2 cut(s) 1350, 1392
SspMI CTAG 7 cut(s) 147, 1176, 1785, 2502, 2508, 2514, 2844
SstI GAGCTC 4 cut(s) 225, 399, 993, 1634
StyD4I CCNGG 9 cut(s) 262, 489, 633, 1508, 2090, 2457, 2696, 2799, 2838
StyI CCWWGG 3 cut(s) 1600, 1697, 2033
TaaI ACNGT 2 cut(s) 248, 811
TaiI ACGT 2 cut(s) 120, 480
TaqII GACCGA 1 cut(s) 854
TatI WGTACW 2 cut(s) 1644, 2504
TauI GCSGC 7 cut(s) 125, 160, 314, 376, 543, 719, 722
Tru1I TTAA 8 cut(s) 380, 765, 1346, 1890, 2082, 2529, 2756, 3042
Tru9I TTAA 8 cut(s) 380, 765, 1346, 1890, 2082, 2529, 2756, 3042
TscAI CASTG 6 cut(s) 527, 649, 814, 1333, 1903, 1984
TseFI GTSAC 4 cut(s) 103, 1751, 2420, 3086
Tsp45I GTSAC 4 cut(s) 103, 1751, 2420, 3086
TspGWI ACGGA 1 cut(s) 502
TspRI CASTG 6 cut(s) 527, 649, 814, 1333, 1903, 1984
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 1704, 1849
VspI ATTAAT 1 cut(s) 1346
XapI RAATTY 3 cut(s) 1239, 1735, 2327
XceI RCATGY 3 cut(s) 1613, 2008, 2156
XcmI CCANNNNNNNNNTGG 2 cut(s) 1100, 2407
XhoI CTCGAG 2 cut(s) 218, 398
XmiI GTMKAC 1 cut(s) 3064
XmnI GAANNNNTTC 4 cut(s) 1718, 2049, 2169, 2385
XspI CTAG 7 cut(s) 147, 1176, 1785, 2502, 2508, 2514, 2844
ZrmI AGTACT 1 cut(s) 2506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.